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Scanning And Transmission Electron Microscopic Studies On The Oviducts Of Pekin Ducks Fed Methyl Mercury Containing Diets, A. Balachandran, M. K. Bhatnagar, H. D. Geissinger

Scanning Electron Microscopy

This study was undertaken to examine the effects of varying levels of methyl mercury (MeHg) on the ultrastructure of the surface epithelium of the oviduct of ducks. Accordingly, Pekin ducks were maintained on feed containing varying doses of (0.0; 0.5; 5.0; 15.0 ppm) of MeHg (Group I - control to IV) for 12 weeks and sacrificed. Tissue from the magnum and the shell gland regions of the oviduct was processed for scanning electron microscopy (SEM) and transmission electron microscopy (TEM). It was found that the primary and secondary folds of these regions of the oviducts of the control and 0.5 …

An Ultrastructural Analysis Of The Physical Organization Of Collagenous (Type I) Matrices: One Determinant Of Urothelium Maintenance In Vitro, G. M. Hodges, C. Rowlatt, A. R. Howlett, L. K. Trejdosiewicz

Scanning Electron Microscopy

Collagenous matrices, used as cell culture substrata, can be prepared from different collagen types in a variety of forms using a range of polymerization procedures. Type I collagen has been most frequently used either as dried collagen films or hydrated collagen gels. Sheets of isolated bladder urothelium, when plated onto such matrices prepared from type I collagen by different polymerization methods (eg. air-drying; NaOH; NaCl; NH₃; or NH₃ followed by glutaraldehyde crosslinking) demonstrate the capability of urothelial cells to attach to a variety of differently prepared matrices irrespective of polymerization procedure. In contrast, both cell proliferation and …

The Influence Of Different Cryopreparations On The Distribution Of Ions In Bullfrog Myocard Cells, R. Meyer, M. Schmitz, K. Zierold

Scanning Electron Microscopy

Bullfrog heart muscle trabecula are shock-frozen in liquid propane cooled by liquid nitrogen and then processed for X-ray microanalysis in two different ways: 1. Freeze-drying followed by vacuum embedding. 2. Cryoultramicrotomy and freeze-drying.

Stained sections of freeze-dried, embedded tissue exhibit detailed ultrastructure, but are useless for X-ray microanalysis. Unstained, dry cut plastic-sections are suitable for X-ray microanalysis, but the ultrastructure appears faint. Higher electron optical contrast and peak-to-background ratio of X-ray spectra are generally obtained in freeze-dried cryosections. Both preparation methods show that the X-ray spectra are influenced by the quality of cryofixation. The phosphorus/potassium ratio in nuclei increases with …

Utah Science Vol. 45 No. 3, Fall 1984

Utah Science

63 ELDERLY MIGRATION PATTERNS IN UTAH W. F. Stinner and H. Y. Nam Elderly Utah residents in 1980 less likely to have migrated and to have originated outside the west, especially long-distance migrants, are more likely to be younger and women than elderly non-migrants.

66 PRECIPITATION VS. WHEAT YIELDS R. F. Nielson and R. L. Cartee Weather affects wheat yields since moisture plays an important factor in limiting dryland wheat production in Utah.

68 THE ART OF HOME CANNING V. T. Mendenhall Home preservation of foods through the techniques of canning, dehydration, fermenting, freezing, or curing continues to be an …

Endometrium Cell Surface Abnormalities In The Syrian Hamster As A Result Of In Utero Exposure To Diethylstilbestrol, J. Gilloteaux, A. W. Steggles

Scanning Electron Microscopy

Scanning electron microscopy (SEM) was used to observe changes in the hamster endometrium cell surface following in utero pre- and/or postnatal exposure to diethylstilbestrol (DES). Some of the changes in cell surfaces are associated with alterations in cell sizes and shapes (from columnar to cuboidal and/or squamous) and in microvilli and mucous secretion. In all cases, DES treated uteri show mucosal cell surface pleomorphism, apocrine secretion and cystic accumulation of secretory material. Microvillous pleomorphism and peculiar linkages attaching one microvillus to others were investigated. Although the function and nature of such linkages is unclear, their presence seems to be more …

The Role Of The Vessels In The Growth Plate: Morphological Examination, K. Draenert, Y. Draenert

Scanning Electron Microscopy

Combined methods of light microscopy to present ossification zones by means of fluorochrome dyes make it possible to explain the contradicting presentations of the vascular system of the growth plate in the literature. The vascular system was casted with methacrylates which can be presented in the scanning electron microscope 3-dimensionally together with the trabeculae as a result of their resistance in the electron beam.

The 3-dimensional presentation in the electron microscope allows a clear distinction between the various vascular sections in the arterial flow system. In the microcorrosion casts the vessels of the epiphyseal side of the growth plate can …

Sixth Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

The Utah Statesman, August 10, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Socio-Economic Considerations In Irrigation Development, Allen Lebaron

Economic Research Institute Study Papers

The contradiction inherent in a potentially hydraulic landscape is manifest. Such a landscape has an insufficient rainfall or none at all, but it possesses other accessible sources of water supply. If man decides to utilize such sources, he may transform dry lands into fertile fields and gardens. He may, but will he? What makes him engage in an adventure which involves great effort and which is fraught with highly problematic institutional consequences?

Progress Report For The Dairy Research Advisory Board, Various Authors

Other Documents

No abstract provided.

The Utah Statesman, July 20, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

The Utah Statesman, June 18, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Utah Science Vol. 45 No. 2, Summer 1984

Utah Science

33 DRAIN ENVELOPES L. S. Willardson Research efforts to improve drainage conditions with the assurance that installed drains will function in a trouble-free manner for a long period of time is continuing at USU.

36 TOUGH TURFS FOR UTAH W. F. Campbell and W. A. Varga Turfs are important in human activities because of their functional, recreational and aesthetic aspects. Although there are hundreds of different species of grasses, learn how to choose the one that is best suited to your region.

41 TRACKING THE SUNFLOWER BEES L. M. Brown and F. D. Parker The USDA Bee Laboratory, situated at …

The Utah Statesman, May 21, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

The Utah Statesman, May 16, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

The Utah Statesman, May 14, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

The Utah Statesman, May 11, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

The Utah Statesman, May 9, 1984, Utah State University

The Utah Statesman

Weekly student newspaper of Utah State University in Logan.

Nutritional Ecology Of The Northern Pocket Gopher: A Study Of Nitrogen Balance, Miki M. Stuebe

Biology

The northern pocket gopher, Thomomys talpoides, is probably not able to maintain nitrogen (N) balance in winter in northern Utah if it eats only belowground plant material typical of that found in the caches it makes. Gophers burrowing 10 h/day would meet about 81% of their daily N requirement on such a diet, which has a true N digestibility of 49.0%, and a biological value of 90.6%. However, gophers could easily meet N requirements in winter by consuming material only slightly higher in N than cached material. High-N material (both plant and animal matter) is probably available to most …

The Effects Of Altered Prey Availability And Shrub Architecture On Spider Community Parameters: A Field Experiment In A Shrub-Steppe Ecosystem, Kathryn Wing

Biology

The effects of altered prey availability and shrub architecture on spider community parameters were studied in a field experiment in northeastern Utah. Treatments of 2 states of foliage density--"normal" and tied (= high density)--and 2 states of insect attractant-- control and baited--were applied to big sage shrubs (Artemisia tridentata) using a factorial experimental design. Temperature and humidity were not significantly different between the 2 types of shrub architecture. The species array and abundance of shrub insects were not significantly altered by the foliage density treatment. Shrubs with insect attractant had significantly larger insects (as measured by body length) …

The Distribution Of Sialic Acid In Selected Tissues And Body Fluids During The Female Reproductive Cycle, Donald Reed Jensen

Biology

An intriguing and dynamic chapter in the history of biochemical research concerns the acylated derivatives of neuraminic acid, the so-called "sialic acids." These substances have been found to occur naturally as constituents of a wide variety of mucoproteins, mucolipids, and related compounds. Because of their vast distribution throughout the animal kingdom, considerable attention has been focused on the chemical, physical, and biological properties of these acids. Attendant studies have shown the functional integrity of certain of the protein and lipid conjugates to be dependent on the presence of sialic acid residues, thereby assigning physiological importance to the acylated derivatives of …

A Comparison Of The Desiccation Resistances Of Three Species Of Crab Spiders Occuring Sympatrically In Northern Utah, Eric James Zurcher

Biology

Respiration rates, water contents, water loss rates, and abilities to withstand water loss were assessed at 15, 25, and 35 C for adults of three species of crab spiders occuring sympatrically on shrub-steppe vegetation in northern Utah. The three species, Misumenops lepidus (Thorell), Xysticus cunctator (Thorell), and Philodromus histrio (Latreille), differ in their apparent seasonal distributions, with M. lepidus being collected most frequently in spring and fall, X. cunctator appearing most frequently in summer, and P. histrio occuring in all seasons. Rates of oxygen consumption paralleled casual observations of the general rates of activities of these spiders. Hence the generally …

Assessment Of Esterases Variation In Different Strains Of Spotted Alfalfa Aphid Therioaphis Maculata (Buckton) (Homoptera:Aphididae), With Special Reference To Endosulfan Susceptibility And Synergism, Nazar Nowman Hama

Biology

Endosulfan susceptibility and synergism with DEF (S.S.S-tributylphosphorotrithioate) and PB (piperonyl butoxide) for different strains of adult apterous spotted alfalfa aphids were examined in addition to evaluating the correlation between esterases and the aphid's tolerance or resistance to endosulfan. Unsynergized endosulfan LC50 values for populations of spotted alfalfa aphids from near Delta, Utah, Nampa, Idaho or Nyssa, Oregon, were 57.56, 37.51 and 45.60 µg/vial, respectively, while pretreatment with DEF reduced the LC50 values to 3.32, 3.2 and 4.73 µg/vial respectively. PB had either little synergistic or antagonistic effects on the endosulfan toxicity. Senergistic data suggested the dominance of esterases as detoxifying …

Sunlight And Plants, Some Pursuits In Physiological Ecology, Martyn M. Caldwell

Faculty Honor Lectures

A physiological ecologist, as the name suggests, focuses on the interface between plant physiology and the ecology of individual organisms. Yet, one in this field should know something about disciplines ranging from physiology, or in the case of the subject of this lecture, even photochemistry, to the study of entire ecosystems.

Utah State University Commencement, 1984 – Main Campus, Utah State University

Commencement Programs

Ninety-First Annual Commencement of Utah State University

Aerosol Stability Of Reovirus, Alan Jeff Mohr

Biology

Reoviruses type 1 Lang, type 2 Jones and type 3 Dearing were aerosolized with a Collison nebulizer and aged for two and a half hours in an environmentally controlled Dynamic Aerosol Toroid to determine reovirus inactivation rates. Results from this study were used to evaluate reoviruses as indicators of viral aerosol pollution. Reovirus viability was determined at three temperatures (2°C, 14°C and 27°C) and four relative humidities [(RH) 20%, 40%, 60% and 80% RH]. After the aging period potentially infectious viruses (PIV), those with a complete outer capsid, were treated with trypsin and transformed into infectious virus (IV) with an …

An Electron Microscopic Study Of The Gill Chamber Of A Dragonfly Nymph (Odonata:Anisoptera), Patricia Bohm Trostle

Biology

The respiratory chamber of the dragonfly nymph, Aeshna multicolor, was studied by means of scanning and transmission electron microscopy. Transmission electron micrographs enable several cellular types, includig two epithelial types, to be distinguished within the individual gill fold. Epithelial cells located basally on the gill fold show features of an osmoregulatory tissue. Cuticle limiting these cells is thinner than-that of typical epithelium, the plasmalemmae are infolded enclosing numerous mitochondria, and a system of extracellular channels is located basally with mitochondria and glycogen seen in the basal cellular outfoldings. Tracheoles are rare. Epithelial cells more distally placed are typical of …

Arthropod Faunas Of Reseeded Rangelands: Effects Of Vegetation Structure, Steve M. Spangler

Biology

Arthropod faunas in different types of reseeded rangeland vegetation were studied in 1980 and 1981, with an emphasis on sap-feeders and predators. Manipulations of grass density and sagebrush presence/absence were performed in 1981 to assess the relative effects of these 2 factors in producing the observed arthropod faunas. Major sap-feeders of crested wheatgrass (Ayropyron cristatum L.) were Irbisia brachycera (Uhler) and Conostethus americanus (Knight) (Miridae), and Oikraneura carneola (Stal) (Cicadellidae). The major sap-feeder of tall wheatgrass (Thinopyrum panticum (Podp.) Barkworth & Dewey) was Laccocera obesa Van Duzee (Delphacidae). I. brachycera, C. americanus, and L. obesa showed population patterns related to …

Genetic Manipulation Of Rhodopseudomonas Sphaeroides For Lactose Utilization, Brian Stevenson

Biology

The purpose of this work was to construct a DNA vector containing the Escherichia coli lactose operon and devise a method for the subsequent introduction into Rhodopseudomonas sphaeroides. Expertise in methods for the purification and manipulation of DNA was developed and improvements upon methods were devised. PRSF 1010 proved to be a poor cloning vector due to a lack of selectable resistance markers and useable restriction sites. The lac operon was cloned into pBR 325 using either HindIII or BamHI restriction endonucleases. Restriction maps of the resulting plasmids were constructed. Attempts to transform R. sphaeroides with plasmid DNA by established …

Development And Evaluation Of Enzyme Labeled Immunosorbent Assays Using Acetylcholinesterase And Its Application To The Study Of Murine Rotaviral Infection Of Infant Mice, Edwin D. Neas

Biology

An enzyme labeled immunosorbent assay (ELISA) which uses the enzyme acetylcholinesterase (AchE) as the immunoreactant label has been developed. The AchE was detected by a reaction between the hydrolysed substrate acetylthiocholine (AchS) and 5:5-dithiobis-2-nitrobenzoate ion. This reaction was spectrophotometrically followed at a wavelength of 405 nm. The exposed thiol group will also react with a thiol-specific fluorochrome, monobromobimane, to produce fluorescence. AchE was conjugated to immunoglobulin using glutaraldehyde without loss of enzyme or antibody activity. The AchE-ELISA was shown to be equal in activity and sensitivity to two widely used commercial ELISA's at an 8-fold increase in the dilution of …