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Articles 1081 - 1110 of 5245
Full-Text Articles in Entire DC Network
Multitasking Scheduling With Shared Processing, Bin Fu, Yumei Huo, Hairong Zhao
Multitasking Scheduling With Shared Processing, Bin Fu, Yumei Huo, Hairong Zhao
Computer Science Faculty Publications
Recently, the problem of multitasking scheduling has raised a lot of interest in the service industries. Hall et al. (Discrete Applied Mathematics, 2016) proposed a shared processing multitasking scheduling model which allows a team to continue to work on the primary tasks while processing the routinely scheduled activities as they occur. With a team being modeled as a single machine, the processing sharing of the machine is achieved by allocating a fraction of the processing capacity to routine jobs and the remaining fraction, which we denote as sharing ratio, to the primary jobs. In this paper, we generalize this model …
Building Squares With Optimal State Complexity In Restricted Active Self-Assembly, Robert M. Alaniz, David Caballero, Sonya C. Cirlos, Timothy Gomez, Elize Grizzell, Andrew Rodriguez, Robert Schweller, Armando Tenorio, Tim Wylie
Building Squares With Optimal State Complexity In Restricted Active Self-Assembly, Robert M. Alaniz, David Caballero, Sonya C. Cirlos, Timothy Gomez, Elize Grizzell, Andrew Rodriguez, Robert Schweller, Armando Tenorio, Tim Wylie
Computer Science Faculty Publications
Tile Automata is a recently defined model of self-assembly that borrows many concepts from cellular automata to create active self-assembling systems where changes may be occurring within an assembly without requiring attachment. This model has been shown to be powerful even with limited assembly size, but many fundamental questions have yet to be explored. Here, we study the state complexity of assembling n×n squares in seeded Tile Automata systems where growth starts from a seed and tiles attach one at a time, similar to the abstract Tile Assembly Model. We provide optimal bounds for three classes of seeded Tile Automata …
School Social Work 238 Exam Preparation: Resources And Handouts, Leticia Villarreal Sosa, Tiffany Nelson, Brenda Coble-Lindsey, Annette Johnson, Giesela Grumbach
School Social Work 238 Exam Preparation: Resources And Handouts, Leticia Villarreal Sosa, Tiffany Nelson, Brenda Coble-Lindsey, Annette Johnson, Giesela Grumbach
School of Social Work Faculty Publications
No abstract provided.
Effect Of Different Pre-Growth Temperatures On The Survival Kinetics Of Salmonella Enterica And Listeria Monocytogenes In Fresh-Cut Salad During Refrigerated Storage, Avninder Kaur, Veerachandra K. Yemmireddy
Effect Of Different Pre-Growth Temperatures On The Survival Kinetics Of Salmonella Enterica And Listeria Monocytogenes In Fresh-Cut Salad During Refrigerated Storage, Avninder Kaur, Veerachandra K. Yemmireddy
School of Earth, Environmental, & Marine Sciences Faculty Publications
The effect of the pre-growth temperature of bacterial cultures on their subsequent survival kinetics in fresh-cut produce during refrigerated storage was investigated in this study. Three-strain cocktails of Listeria monocytogenes and Salmonella enterica, cultured at different growth temperatures (4, 21, and 37 °C) were inoculated on fresh-cut mixed salad and on individual produce in the mixed salad. The inoculated samples were stored at 4 °C and 80 ± 2% relative humidity (RH) for up to 72 h and the growth, survival, or death kinetics were determined at regular intervals. The results indicate that depending upon the type of pathogen …
Analytic Distribution Of The Optimal Cross-Correlation Statistic For Stochastic Gravitational-Wave-Background Searches Using Pulsar Timing Arrays, Jeffrey S. Hazboun, Patrick M. Meyers, Joseph D. Romano, Xavier Siemens, Anne M. Archibald
Analytic Distribution Of The Optimal Cross-Correlation Statistic For Stochastic Gravitational-Wave-Background Searches Using Pulsar Timing Arrays, Jeffrey S. Hazboun, Patrick M. Meyers, Joseph D. Romano, Xavier Siemens, Anne M. Archibald
Physics & Astronomy Faculty Publications
We show via both analytical calculation and numerical simulation that the optimal cross-correlation statistic (OS) for stochastic gravitational-wave-background (GWB) searches using data from pulsar timing arrays follows a generalized chi-squared (GX2) distribution—i.e., a linear combination of chi-squared distributions with coefficients given by the eigenvalues of the quadratic form defining the statistic. This observation is particularly important for calculating the frequentist statistical significance of a possible GWB detection, which depends on the exact form of the distribution of the OS signal-to-noise ratio ρˆ ≡ Aˆ 2 gw=σ0 in the absence of GW-induced cross correlations (i.e., the null distribution). Previous discussions of …
Ortho-Fluoro Or Ortho Effect? Oxidative Addition Of Zerovalent Nickel Into The C–Cn Bond Of Substituted Benzonitriles, Tülay Ateşin, William D. Jones, Abdurrahman C. Atesin
Ortho-Fluoro Or Ortho Effect? Oxidative Addition Of Zerovalent Nickel Into The C–Cn Bond Of Substituted Benzonitriles, Tülay Ateşin, William D. Jones, Abdurrahman C. Atesin
School of Earth, Environmental, & Marine Sciences Faculty Publications
A recent study of the oxidative addition of zerovalent Ni to the C–CN bond of F-substituted benzonitriles showed significantly increased stabilization of the C–CN oxidative addition products with o-F groups (−6.6 kcal/mol per o-F) compared to m-F groups (−1.8 kcal/mol per m-F). To answer the question of whether this is an o-F effect or an ortho effect, in this study the effect of CF3 and CH3 groups on the oxidative addition of the [Ni(dmpe)] fragment [dmpe = 1,2-bis(dimethylphosphino)ethane] to the C–CN bond of benzonitriles has been studied. A density functional theory study of the reaction …
The Rider (2023-11-13), Natalie Lapsley
Youth In Danger And Students At Risk Of Dropping Out, Mamoona Muzammil, Muzammil Arshad
Youth In Danger And Students At Risk Of Dropping Out, Mamoona Muzammil, Muzammil Arshad
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)
Bullying is any unwanted aggressive behavior(s) by another youth or group of youth that involves an observed or perceived power imbalance and is repeated multiple times or is highly likely to be repeated. Bullying may inflict harm or distress on the targeted youth including physical, psychological, social, or educational harm. Bullying can lead to fear of school, absenteeism, and stunted academic progress, which in turn are precursors to dropping out of school.
- Effects of Bullying on Students Long lasting effects
- School Dropout.
- Lack of Concentration
- Reduced Motivation
- Less Class Participation
- Less Attendance Lower Academic Achievement
Student Dropouts
The increase in …
Cost Analysis Of Adsorption Based Air Capture Of Co2, Mona Muzammil, Muzammil Arshad, Muazzam Arshad
Cost Analysis Of Adsorption Based Air Capture Of Co2, Mona Muzammil, Muzammil Arshad, Muazzam Arshad
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)
Carbon dioxide (CO2) capture and conversion to value added products, such as chemicals, polymers, and carbon-based fuels represents a promising approach to transform a potential threat to the environment into a value-added product for long term sustainability. Emerging Carbon Capture Technologies: Towards a Sustainable Future provides a multidisciplinary view of the research that is being carried out in this field, covering materials and processes for CO2 capture and utilization and including a broad discussion of the impact of novel technologies in carbon capture on the energy landscape, society and climate. Of interest to students, researchers and professionals in industries related …
Employing A Fractional Basis Set To Solve Nonlinear Multidimensional Fractional Differential Equations, Md. Habibur Rahman, Muhammad I. Bhatti, Nicholas Dimakis
Employing A Fractional Basis Set To Solve Nonlinear Multidimensional Fractional Differential Equations, Md. Habibur Rahman, Muhammad I. Bhatti, Nicholas Dimakis
Physics & Astronomy Faculty Publications
Fractional-order partial differential equations have gained significant attention due to their wide range of applications in various fields. This paper employed a novel technique for solving nonlinear multidimensional fractional differential equations by means of a modified version of the Bernstein polynomials called the Bhatti-fractional polynomials basis set. The method involved approximating the desired solution and treated the resulting equation as a matrix equation. All fractional derivatives are considered in the Caputo sense. The resulting operational matrix was inverted, and the desired solution was obtained. The effectiveness of the method was demonstrated by solving two specific types of nonlinear multidimensional fractional …
Introductory Chemistry, Mona Muzammil
Introductory Chemistry, Mona Muzammil
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)
Introductory Chemistry, this edition is designed for a one-semester, introductory or preparatory chemistry course. Students taking this course need to develop problem-solving skills-but they also must see why these skills are important to them and to their world. Introductory Chemistry extends chemistry from the laboratory to the student's world. It motivates students to learn chemistry by demonstrating how it plays out in their daily lives.
This book draws students into the course through engagement and building their foundational knowledge – while introducing new content and resources to help students build critical thinking and problem-solving skills. allowing students flexibility and ensuring …
Feature Selection In Intrusion Detection Systems: A New Hybrid Fusion Of Bat Algorithm And Residue Number System, Yakub Kayode Saheed, Temitope Olubanjo Kehinde, Mustafa Ayobami Raji, Usman Ahmad Baba
Feature Selection In Intrusion Detection Systems: A New Hybrid Fusion Of Bat Algorithm And Residue Number System, Yakub Kayode Saheed, Temitope Olubanjo Kehinde, Mustafa Ayobami Raji, Usman Ahmad Baba
Marketing Faculty Publications
This research introduces innovative approaches to enhance intrusion detection systems (IDSs) by addressing critical challenges in existing methods. Various machine-learning techniques, including nature-inspired metaheuristics, Bayesian algorithms, and swarm intelligence, have been proposed in the past for attribute selection and IDS performance improvement. However, these methods have often fallen short in terms of detection accuracy, detection rate, precision, and F-score. To tackle these issues, the paper presents a novel hybrid feature selection approach combining the Bat metaheuristic algorithm with the Residue Number System (RNS). Initially, the Bat algorithm is utilized to partition training data and eliminate irrelevant attributes. Recognizing the Bat …
Rigidity Of Ext And Tor Via Flat–Cotorsion Theory, Lars Winther Christensen, Luigi Ferraro, Peder Thompson
Rigidity Of Ext And Tor Via Flat–Cotorsion Theory, Lars Winther Christensen, Luigi Ferraro, Peder Thompson
School of Mathematical & Statistical Sciences Faculty Publications
Let p be a prime ideal in a commutative noetherian ring R and denote by k(p) the residue field of the local ring R_p. We prove that if an R-module M satisfies Ext_R^n(k(p),M) = 0 for some n >= dim R, then Ext_R^i(k(p),M) = 0 holds for all i >= n. This improves a result of Christensen, Iyengar, and Marley by lowering the bound on n. We also improve existing results on Tor-rigidity. This progress is driven by the existence of minimal semi-flat-cotorsion replacements in the derived category as recently proved by Nakamura and Thompson.
Experimental Studies On The Operation Of Agricultural Crops Mowing Unit With Simultaneous Chopping And Incorporation Of Stubble Into The Soil, Volodymyr Nadykto, Sergey Kiurchev, Tatiana Chorna, Taras Hutsol, Szymon Głowacki, Anatoliy Rud, Alona Shevtsova, Igor Ryabov, Katarzyna Szwedziak
Experimental Studies On The Operation Of Agricultural Crops Mowing Unit With Simultaneous Chopping And Incorporation Of Stubble Into The Soil, Volodymyr Nadykto, Sergey Kiurchev, Tatiana Chorna, Taras Hutsol, Szymon Głowacki, Anatoliy Rud, Alona Shevtsova, Igor Ryabov, Katarzyna Szwedziak
Sociology Faculty Publications
For harvesting crops, one-phase and two-phase methods are most often used. When implementing the second one, mounted and trailed units are used. The latter is becoming less and less used due to the problems with movement stability in the horizontal plane because of the asymmetric layout. A stubble background, which is susceptible to solar and wind influences, is formed as a consequence of application of the mounted harvesting unit, As a result, the field’s soil in the inter-swath space intensively loses moisture. A harvesting unit based on a tractor with rear steerable wheels has been designed to eliminate this shortcoming. …
Interactions Of Traveling Waves In Low Order Approximations For The Two-Dimensional Euler Equations, Julio C. Paez, Zhijun Qiao, Vesselin Vatchev
Interactions Of Traveling Waves In Low Order Approximations For The Two-Dimensional Euler Equations, Julio C. Paez, Zhijun Qiao, Vesselin Vatchev
School of Mathematical & Statistical Sciences Faculty Publications
In this paper, we study traveling waves with elastic and inelastic interactions in a low order of approximation. The waves are derived in quadratic or linear order of approximation from the two-dimensional Euler equations. A new type of inelastic interaction that is closely related to numerical study observations of the Euler equations is obtained in a linear order.
Amplitude-Voltage Response Of Parametric Resonance Of Electrostatically Actuated Circular Membranes, Marcos Alipi, Dumitru I. Caruntu
Amplitude-Voltage Response Of Parametric Resonance Of Electrostatically Actuated Circular Membranes, Marcos Alipi, Dumitru I. Caruntu
Mechanical Engineering Faculty Publications
This paper deals with the amplitude-voltage response of parametric resonance of electrostatically actuated MEMS circular membranes resonator for sensing applications. The system consists of a flexible circular membrane over a parallel ground plate. A soft AC voltage is applied between the MEMS membrane and ground plate to actuate the membrane near its first natural frequency. Two methods of investigation are used in this research, namely the method of multiple scales (MMS), and the reduced order model (ROM). These methods were used to solve for the voltage response of the MEMS analytically and numerically. This work also reports the effects the …
A Fast And Responsive Web-Based Framework For Visualizing Hpc Application Usage, Ved Arora, Nayeli Gurrola, Amiya K. Maji, Guangzhen Jin
A Fast And Responsive Web-Based Framework For Visualizing Hpc Application Usage, Ved Arora, Nayeli Gurrola, Amiya K. Maji, Guangzhen Jin
Computer Science Faculty Publications
Insights about applications and user environments can help HPC center staff make data-driven decisions about cluster operations. In this paper, we present a fast and responsive web-based visualization framework for analyzing HPC application usage. By leveraging XALT, a powerful tool for tracking application and library usage, we collected tens of millions of data points on a national supercomputer. The portable visualization framework created with Plotly Dash can be easily launched as a container and accessed from a web browser. The presented visualizations take a deep dive into the XALT data, analyzing application use, compiler usage, library usage, and even user-specific …
Spectrum-Enhanced Trca (Se-Trca): A Novel Approach For Direction Detection In Ssvep-Based Bci, Amirmohammad Mijani, Mohammad Norizadeh Cherloo, Haoteng Tang, Liang Zhan
Spectrum-Enhanced Trca (Se-Trca): A Novel Approach For Direction Detection In Ssvep-Based Bci, Amirmohammad Mijani, Mohammad Norizadeh Cherloo, Haoteng Tang, Liang Zhan
Computer Science Faculty Publications
The Steady State Visual Evoked Potential (SSVEP) is a widely used component in BCIs due to its high noise resistance and low equipment requirements. Recently, a novel SSVEP-based paradigm has been introduced for direction detection, in which, unlike the common SSVEP paradigms that use several frequency stimuli, only one flickering stimulus is used and it makes direction detection very challenging. So far, only the CCA method has been used for direction detection using SSVEP component analysis. Since Canonical Correlation Analysis (CCA) has some limitations, a Task-Related Component Analysis (TRCA) based method has been introduced for feature extraction to improve the …
Quality And Reliability Of Spanish-Language Youtube Videos On Breast Cancer, Fernando C. Diaz, Michael Lapelusa, Hansapani Rodrigo, Ismael Polo Perez, Dailis S. Corria Cedeno, Henry Kwang
Quality And Reliability Of Spanish-Language Youtube Videos On Breast Cancer, Fernando C. Diaz, Michael Lapelusa, Hansapani Rodrigo, Ismael Polo Perez, Dailis S. Corria Cedeno, Henry Kwang
School of Medicine Publications
As the number of Spanish- speaking patients with cancer in the USA grows, it is vital that reliable online breast cancer information is available in Spanish. The main objective of our study was to determine the quality and reliability of online videos in Spanish regarding breast cancer. We conducted a YouTube search using the phrase "cancer de mama" (breast cancer) and graded their quality and reliability using the Journal of the American Medical Association (JAMA) Benchmark Criteria, Global Quality Score (GQS), and the Modified DISCERN Questionnaire. Videos were characterized by descriptive variables. Videos were primarily from Spain (36.4%) and Mexico …
Geometric Aspects Of Quantization And Relationship To Integrability, Paul Bracken
Geometric Aspects Of Quantization And Relationship To Integrability, Paul Bracken
School of Mathematical & Statistical Sciences Faculty Publications
It is the case that quantum mechanics has a deep geometric structure and can be presented accordingly. Quantum mechanics is to a certain degree foreshadowed by the geometry inherent in the geometric structure of classical mechanics. The purpose here is to present some new results and proofs which impact the mathematical structure of quantum mechanics. The relationship between integrability and quantum physics is investigated in terms of geometric ideas and structures. Two physical examples are drawn from these mathematical ideas which directly relate to physics. An introduction as to how these ideas can be extended to infinite degrees of freedom …
Conceptualizing Ethics, Authenticity, And Efficacy Of Simulations In Teacher Education, Carrie Wilkerson Lee, Liza Bondurant, Bima Sapkota, Heather Howell, Yvonne Lai
Conceptualizing Ethics, Authenticity, And Efficacy Of Simulations In Teacher Education, Carrie Wilkerson Lee, Liza Bondurant, Bima Sapkota, Heather Howell, Yvonne Lai
School of Mathematical & Statistical Sciences Faculty Publications
This working group was a continuation of working groups in 2019 and 2021 that initially aimed to focus on equity in simulations of practice in mathematics teacher education. We began by discussing our conceptualizations of simulations and equity. Next, we reflected on the lack of work that currently exists at the intersection of simulations and equity as well as our limited collective expertise in this space. We proposed the following areas of potential research: Access,Design, Affective Domains, Teaching Practices, Assessment, Critical Conversations. Attendees self-selected into focus groups and met to discuss their current work and how future work could focus …
A Review Of Cyber Attacks On Sensors And Perception Systems In Autonomous Vehicle, Taminul Islam, Md. Alif Sheakh, Anjuman Naher Jui, Omar Sharif, Md Zobaer Hasan
A Review Of Cyber Attacks On Sensors And Perception Systems In Autonomous Vehicle, Taminul Islam, Md. Alif Sheakh, Anjuman Naher Jui, Omar Sharif, Md Zobaer Hasan
School of Mathematical & Statistical Sciences Faculty Publications
Vehicle automation has been in the works for a long time now. Automatic brakes, cruise control, GPS satellite navigation, etc. are all common features seen in today's automobiles. Automation and artificial intelligence breakthroughs are likely to lead to an increase in the usage of automation technologies in cars. Because of this, mankind will be more reliant on computer-controlled equipment and car systems in our daily lives. All major corporations have begun investing in the development of self-driving cars because of the rapid advancement of advanced driver support technologies. However, the level of safety and trustworthiness is still questionable. Imagine what …
Mems Cantilever Biosensors Driven By Fringe Effect: Voltage-Amplitude Response Of Parametric Resonance, Miguel A. Martinez, Dumitru I. Caruntu
Mems Cantilever Biosensors Driven By Fringe Effect: Voltage-Amplitude Response Of Parametric Resonance, Miguel A. Martinez, Dumitru I. Caruntu
Mechanical Engineering Faculty Publications
This paper deals with voltage-amplitude response of fringing field/effect actuated micro-electromechanical system (MEMS) cantilever beam resonator biosensors at parametric resonance. The system consists of a flexible electrode (cantilever beam) and a fixed electrode (ground plate) in parallel, connected through an AC voltage source. The electrostatic force induced is due to the parallel-plate capacitance, which is directly related to the overlapping area of the two electrodes (Palmer’s Formula). In addition, fringing field electrostatic actuation must be accounted for. This is due to the bending of electric field lines from the ground plate to the top and side walls of the cantilever …
A Comprehensive Survey Of Complex Brain Network Representation, Haoteng Tang, Guixiang Ma, Yanfu Zhang, Kai Ye, Lei Guo, Guodong Liu, Qi Huang, Yalin Wang, Olusola Ajilore, Alex D. Leow
A Comprehensive Survey Of Complex Brain Network Representation, Haoteng Tang, Guixiang Ma, Yanfu Zhang, Kai Ye, Lei Guo, Guodong Liu, Qi Huang, Yalin Wang, Olusola Ajilore, Alex D. Leow
Computer Science Faculty Publications
Highlights
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Major traditional and deep learning methods on brain network representation are overviewed.
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Brain network datasets and algorithm implementation tools are summarized.
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Promising research directions in brain network analysis are discussed.
Abstract
Recent years have shown great merits in utilizing neuroimaging data to understand brain structural and functional changes, as well as its relationship to different neurodegenerative diseases and other clinical phenotypes. Brain networks, derived from different neuroimaging modalities, have attracted increasing attention due to their potential to gain system-level insights to characterize brain dynamics and abnormalities in neurological conditions. Traditional methods aim to pre-define multiple topological features of brain …
Confirmation Of N-Hexane As An Inert Co-Solvent In The Production Of Functionalized Silicon Nanoparticles From Reactive High-Energy Ball Milling, Julie P. Vanegas, Amanda Reusch, Mark J. Fink, Brian S. Mitchell
Confirmation Of N-Hexane As An Inert Co-Solvent In The Production Of Functionalized Silicon Nanoparticles From Reactive High-Energy Ball Milling, Julie P. Vanegas, Amanda Reusch, Mark J. Fink, Brian S. Mitchell
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Chemistry)
Alkanes such as n-hexane have been used as co-solvents in the production of functionalized semiconductor nanoparticles from alkenes and alkynes using Reactive High Energy Ball Milling (RHEBM) under the assumption that they are non-reactive under typical milling conditions. In this paper, we report on a comparative study with two hydrocarbon solvents of comparable chain length, 1-hexyne, and n-hexane, and their milling products using three different commercially available silicon precursors, namely single crystal silicon wafers and polycrystalline particles having a nominal size of 4 μm and 1 mm. We found that nanoparticle formation and surface functionalization in all the three silicon …
Aspects Of Non-Associative Gauge Theory, Sergey Grigorian
Aspects Of Non-Associative Gauge Theory, Sergey Grigorian
School of Mathematical & Statistical Sciences Faculty Publications
A smooth loop is the direct non-associative generalization of Lie group. In this paper, we review the theory of smooth loops and smooth loop bundles. This is then used to define a non-associative analog of the Chern-Simons functional.
Broadband Quantum Enhancement Of The Ligo Detectors With Frequency-Dependent Squeezing, D. Ganapathy, W. Jia, M. Nakano, V. Xu, N. Aritomi, T. Cullen, N. Kijbunchoo, S. E. Dwyer, Francisco Llamas, Volker Quetschke
Broadband Quantum Enhancement Of The Ligo Detectors With Frequency-Dependent Squeezing, D. Ganapathy, W. Jia, M. Nakano, V. Xu, N. Aritomi, T. Cullen, N. Kijbunchoo, S. E. Dwyer, Francisco Llamas, Volker Quetschke
Physics & Astronomy Faculty Publications
Quantum noise imposes a fundamental limitation on the sensitivity of interferometric gravitational-wave detectors like LIGO, manifesting as shot noise and quantum radiation pressure noise. Here, we present the first realization of frequency-dependent squeezing in full-scale gravitational-wave detectors, resulting in the reduction of both shot noise and quantum radiation pressure noise, with broadband detector enhancement from tens of hertz to several kilohertz. In the LIGO Hanford detector, squeezing reduced the detector noise amplitude by a factor of 1.6 (4.0 dB) near 1 kHz; in the Livingston detector, the noise reduction was a factor of 1.9 (5.8 dB). These improvements directly impact …
Lógica, Lenguajes Formales Y Modalidad [Logic, Formal Languages And Modality], Otávio Bueno, Melisa Vivanco
Lógica, Lenguajes Formales Y Modalidad [Logic, Formal Languages And Modality], Otávio Bueno, Melisa Vivanco
Philosophy Faculty Publications
Este artículo examina dos supuestas limitaciones en el uso de lenguajes formales: por un lado, las compensaciones entre el poder expresivo e inferencial y, por el otro, el fenómeno del encarcelamiento del sistema. Después de reconceptualizar el tema, consideramos el papel que desempeña la modalidad en la comprensión de ciertos aspectos de las estructuras matemáticas y defendemos su centralidad.
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This paper examines two alleged limitations in the use of formal languages: on the one hand, the trade-offs between expressive and inferential power, and on the other, the phenomenon of system imprisonment. After reconceptualizing the issue, we consider the role …
The Rider (2023-10-23), Natalie Lapsley
A Framework To Validate Fluorescently Labeled Dna-Binding Proteins For Single-Molecule Experiments, Miranda L. Molina, Lindsey E. Way, Zhongqing Ren, Qin Liao, Bianca Guerra, Brandon Shields, Xindan Wang, Hyeongjun Kim
A Framework To Validate Fluorescently Labeled Dna-Binding Proteins For Single-Molecule Experiments, Miranda L. Molina, Lindsey E. Way, Zhongqing Ren, Qin Liao, Bianca Guerra, Brandon Shields, Xindan Wang, Hyeongjun Kim
Physics & Astronomy Faculty Publications
Due to the enhanced labeling capability of maleimide-based fluorescent probes, lysine-cysteine-lysine (KCK) tags are frequently added to proteins for visualization. In this study, we employed an in vitro single-molecule DNA flow-stretching assay as a sensitive way to assess the impact of the KCK tag on the property of DNA-binding proteins. Using Bacillus subtilis ParB as an example, we show that, although no noticeable changes were detected by in vivo fluorescence imaging and chromatin immunoprecipitation (ChIP) assays, the KCK tag substantially altered ParB's DNA compaction rates and its response to nucleotide binding and to the presence of the specific sequence (parS) …