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Lightweight And Resilient Cryptographic Protocols For Next-Generation Networking Systems, Saif Eddine Nouma Mar 2026

Lightweight And Resilient Cryptographic Protocols For Next-Generation Networking Systems, Saif Eddine Nouma

USF Tampa Graduate Theses and Dissertations

The security of our increasingly interconnected world is strongly dependent on the strength and practicality of the cryptographic tools we deploy. As billions of embedded devices join the Internet of Things (IoT) and interact with remote cloud infrastructures for data processing, analysis, and actuation, they evolve into so-called digital twins. This rapid expansion introduces numerous attack vectors, particularly targeting constrained IoT devices, which greatly increase the attack surface and often become the root cause of large-scale infrastructure compromises. At the same time, the rise of quantum computing threatens the hardness of today’s intractability assumptions that underpin cryptographic primitives currently deployed. …


Framing The Pressure: Mental Health Representation In Olympic Athletes Through The Weight Of Gold, Daniel B. Gearin Mar 2026

Framing The Pressure: Mental Health Representation In Olympic Athletes Through The Weight Of Gold, Daniel B. Gearin

USF Tampa Graduate Theses and Dissertations

This study examines how the HBO documentary The Weight of Gold constructs meaning around athlete mental health through narrative and visual framing. Grounded in framing theory, the purpose of this research is to identify the key themes presented in the film and analyze how cinematic techniques shape audience understanding of mental health in Olympic athletes. The rationale for this study stems from a gap in existing research, as most scholarship focuses on psychological outcomes rather than how media representations construct those meanings. Using a qualitative approach, the study applies thematic analysis to 17 scenes of the documentary, supported by a …


Comfort Beyond Grief: The Influence Of Brazilian Spiritism On Grief, Maria Luiza D. Reynaud Mar 2026

Comfort Beyond Grief: The Influence Of Brazilian Spiritism On Grief, Maria Luiza D. Reynaud

USF Tampa Graduate Theses and Dissertations

This thesis explores the transformative influence of Brazilian Spiritism on the experience of grief, utilizing a qualitative framework of autoethnography and ethnography. The research begins with the author’s personal account of transitioning from paralyzing existential anxiety and a childhood fear of hell and death to a state of spiritual peace fostered by the study of the Spiritist Doctrine. Historically perceived as an unbearable mystery, Spiritism posits life as a continuous continuum where the immortal soul merely sheds the physical body like a cocoon to return to its true home.

Rooted in the 19th-century codification by Allan Kardec, the doctrine is …


Manufacturing Main Street: How Historic Plant City Main Street Revitalizes Its Community And Simulates The Commons, Emily Burke Mar 2026

Manufacturing Main Street: How Historic Plant City Main Street Revitalizes Its Community And Simulates The Commons, Emily Burke

USF Tampa Graduate Theses and Dissertations

Community has remained a central concern to sociology since its founding, even as industrialization, globalization, and digital media have transformed how social ties are formed and maintained. Small American downtowns once anchored civic and social life, but have faced destabilizing events like globalization, the rise of e-commerce, and the COVID-19 pandemic. In response, many small cities have turned to the national nonprofit Main Street AmericaTM to help them revitalize their downtowns. Although research on small cities is limited compared to large urban centers, emerging literature highlights post-COVID opportunities to renew physical and local shopping on main street (small city downtowns). …


Understanding How Single Mothers In Southwest Nigeria Navigate Mental Health Challenges And Financial Hardships: A Culture-Centered Approach, Zulykha B. Akinjobi Mar 2026

Understanding How Single Mothers In Southwest Nigeria Navigate Mental Health Challenges And Financial Hardships: A Culture-Centered Approach, Zulykha B. Akinjobi

USF Tampa Graduate Theses and Dissertations

Single motherhood is a growing yet stigmatized family structure in Southwest Nigeria, where patriarchal norms, cultural expectations, and structural inequalities converge to produce unique mental health and financial challenges for women. This qualitative study examines how single mothers in Southwest Nigeria navigate mental health challenges and financial hardships through the lens of the Culture-Centered Approach (CCA). Drawing on in-depth semi-structured interviews with 20 single mothers residing in the Yoruba-speaking region of Southwest Nigeria, this study foregrounds locally situated narratives to understand how these women construct and reconstruct meanings of single motherhood within their cultural and structural contexts. Findings reveal that …


Crow's Nest, March 9, 2026, Crow's Nest Staff Mar 2026

Crow's Nest, March 9, 2026, Crow's Nest Staff

Crow's Nest

The March 9, 2026 issue of Crow's Nest.


Human-Ai Collaboration In Marketing Brainstorming: A Meta-Analytic Investigation Of Implementation Approaches And Creative Outcomes, Michael Michaels Mar 2026

Human-Ai Collaboration In Marketing Brainstorming: A Meta-Analytic Investigation Of Implementation Approaches And Creative Outcomes, Michael Michaels

USF Tampa Graduate Theses and Dissertations

Marketing organizations face intensifying pressure to sustain creative output while managing constrained resources and accelerating digital content cycles. The integration of artificial intelligence into marketing brainstorming processes presents a promising response to this challenge; however, prior to this investigation, no comprehensive quantitative synthesis had established the overall effectiveness of human-AI collaboration in marketing creativity contexts or identified optimal implementation strategies.

This dissertation presents a systematic meta-analytic investigation of AI-enhanced brainstorming effectiveness in marketing contexts. Following PRISMA guidelines, 42 independent empirical studies (N = 2,166 participants) published between 2018 and 2025 were identified across four primary academic databases, coded for implementation …


Aging In The Shadows: Szekler Cemeteries And The Case For Recognizing Elderly Populations In Bioarchaeology, Katie M. Mcgrath Mar 2026

Aging In The Shadows: Szekler Cemeteries And The Case For Recognizing Elderly Populations In Bioarchaeology, Katie M. Mcgrath

USF Tampa Graduate Theses and Dissertations

The study of aging in past populations lies at the intersection of bioarchaeology and paleodemography, offering insight into both biological processes and lived social experience. Despite this potential, older adults are often rendered “invisible” in skeletal assemblages due to methodological and cultural biases. This thesis addresses this gap through a rare, community-based study of aging among the Székely population of Transylvania. By integrating osteological analysis of individuals (11th–19th centuries) from the archaeological Papdomb church cemetery with demographic data derived from the modern cemetery headstones (ca.1850–present), this research examines patterns of aging, longevity, and elder visibility across a culturally and spatially …


Behind The Bar: How Bartenders And Regulars Co-Produce Guardianship In Neighborhood Bars, Jack I. Wexler Mar 2026

Behind The Bar: How Bartenders And Regulars Co-Produce Guardianship In Neighborhood Bars, Jack I. Wexler

USF Tampa Graduate Theses and Dissertations

Although bars are commonly conceptualized as sites of disorder and violence, they are also spaces where social order is routinely produced and maintained. Bars are frequent targets of crime prevention efforts because they tend to concentrate aggression, disorder, and crime; yet little is known about how barroom actors—owners, bartenders, and regular patrons—actively maintain order. Explanations typically emphasize (a) physical features of bars (layout, lighting, crowding, door policies), (b) individual and situational predictors (clientele mix, intoxication levels), and (c) the actions of formal controllers—owners, bartenders, and bouncers. I consider an additional, and overlooked informal controller: the bar regular. This qualitative, exploratory …


Examining Factors Associated With Higher Percentages Of Women In Executive Leadership Positions In College Sport, Kelly Jean Brock Mar 2026

Examining Factors Associated With Higher Percentages Of Women In Executive Leadership Positions In College Sport, Kelly Jean Brock

USF Tampa Graduate Theses and Dissertations

The underrepresentation of women in executive leadership roles within intercollegiate athletics remains a persistent issue despite decades of progress in gender equity following the passage of Title IX. The purpose of this study was to examine the institutional and organizational factors associated with higher percentages of women in executive leadership positions within NCAA Division I Football Bowl Subdivision (FBS) athletic departments. Specifically, this research investigated whether institutional characteristics, such as athletic department budget size, the gender of the athletic director, the gender of the university president or chancellor, and institutional commitments to diversity, equity, and inclusion were associated with the …


J.K. Rowling’S Loss Of Control: Authorial Anxieties Within The Actor Network, Casey Sausville Mar 2026

J.K. Rowling’S Loss Of Control: Authorial Anxieties Within The Actor Network, Casey Sausville

USF Tampa Graduate Theses and Dissertations

This thesis investigates how traditional ideas of authorship are contradicted in the 21st century as authorial power is distributed through an actor network. In this case study, I apply Bruno Latour’s Actor Network Theory (ANT) to the authorship of the fantasy series Harry Potter, in which J.K. Rowling, Warner Bros, and the fandom are all among the co-creators. In doing so, I find that when power has been exercised by other actors in the network, this has in multiple cases been experienced by Rowling as a threat to her security as an author, a woman, and a mother. This is …


Growth Mindset And Practice Strategy In Expert Pianists, Yiling Li Mar 2026

Growth Mindset And Practice Strategy In Expert Pianists, Yiling Li

USF Tampa Graduate Theses and Dissertations

Growth mindset is often theorized to support persistence, strategic learning, and reflective regulation, yet little is known about whether growth mindset differentiates how expert pianists organize and evaluate their practice strategies. The purpose of this dissertation was to examine whether expert pianists’ growth mindset beliefs relate to multidimensional practice strategy effectiveness and usage and to metacognitive indicators of adaptive regulation, including strategy adaptability, self-monitoring effectiveness, confidence in strategy selection, and the illusion of fluency (feeling well prepared in practice yet underperforming in performance). Participants were 90 expert pianists (piano faculty members at North American universities classified as Research 1) who …


Exploring Graduate Nursing Students’ Learning Experiences, Perceived Cognitive Loads, And Attitudes Toward Immersive Virtual Reality (Ivr) In Clinical Training Environments, Pranam Parsanlal Mar 2026

Exploring Graduate Nursing Students’ Learning Experiences, Perceived Cognitive Loads, And Attitudes Toward Immersive Virtual Reality (Ivr) In Clinical Training Environments, Pranam Parsanlal

USF Tampa Graduate Theses and Dissertations

Simulation-based education (SBE) is a widely used learning method in nursing education to develop clinical skills, while in recent years, immersive virtual reality (IVR) has become a sought-after SBE method. There is a significant need to understand how to support IVR integration. Previous research suggests exploring students’ perceptions and experiences toward IVR holistically and comprehensively to understand how to integrate IVR into nursing educational practices. This explanatory sequential mixed methods research study aimed to explore the learning experiences, perceived cognitive loads, and attitudes toward IVR of 11 graduate nursing students in an IVR clinical training environment. To explore perceptions, students …


Response Of Botovskaya Cave To Local And Regional Environmental Dynamics, Jade Margerum, Oksana Gutareva, Franziska Lechleitner, Maria Box, Stuart Umbo, Anton Vaks, Hanno Meyer, Aleksandr Kononov, Aleksander Osintsev, Dimitri Sokol'nikov, Norbert Marwan, Ola Kwiecien, Sebastian Breitenbach Mar 2026

Response Of Botovskaya Cave To Local And Regional Environmental Dynamics, Jade Margerum, Oksana Gutareva, Franziska Lechleitner, Maria Box, Stuart Umbo, Anton Vaks, Hanno Meyer, Aleksandr Kononov, Aleksander Osintsev, Dimitri Sokol'nikov, Norbert Marwan, Ola Kwiecien, Sebastian Breitenbach

International Journal of Speleology

Speleothems from caves in near-permafrost regions can provide unique insights into the climatic history of temperature-sensitive environments. These regions are often remote and only seasonally accessible, which makes cave monitoring – a requirement for speleothem-based proxy interpretation – challenging. We document ventilation, the infiltration regime, and the isotopic composition of dripwater in Botovskaya Cave in southern Siberia, a host to speleothems previously used to reconstruct Quaternary permafrost dynamics in continental Eurasia. We explore surface and cave temperature records, cave air CO2 concentration data and stable isotopes in precipitation and dripwater and evaluate infiltration conditions and the sensitivity of speleothems …


Numerals And Other Means Of Expressing Plurality In Language And Thought: A Developmental Perspective Across Cultures., Giovanni Giuseppe G.G. Nicosia Prof. Mar 2026

Numerals And Other Means Of Expressing Plurality In Language And Thought: A Developmental Perspective Across Cultures., Giovanni Giuseppe G.G. Nicosia Prof.

Numeracy

Several ancient languages use linguistic structures to differentiate between singular, dual, and plural forms that are used when one, two, or many objects are referenced. (Some languages go further, adding a trial form to differentiate two from three.) In many modern languages, numerals are used to convey precise number. After reviewing a range of ways languages express number (including the interesting case of Pirahã which does not express precise number), the paper concludes with a proposed developmental path for the expression of quantity in languages.


Ice Temperature And Heat Transfer Processes In Cave Glaciers, Aurel Persoiu Mar 2026

Ice Temperature And Heat Transfer Processes In Cave Glaciers, Aurel Persoiu

International Journal of Speleology

Cave glaciers (i.e., perennial ice accumulations in caves), found throughout mountainous regions in Europe and North America are at risk of extinction due to rising air temperature and altered rainfall patterns. Their formation and subsequent preservation is the result of accumulation of ice during winter months and limited ablation in summer. These processes, in turn, are controlled by air temperature, which determines the overall mass balance of ice. One important aspect is the heat the ice mass loses in winter, which increases its resilience during summer. While ice caves’ climate has been intensively studied in the past, investigations of ice …


Youmatter: A Nursing Workforce Study, Stacy L. Pryor Mar 2026

Youmatter: A Nursing Workforce Study, Stacy L. Pryor

USF Tampa Graduate Theses and Dissertations

The sustainability and health of the nursing profession are immediate and pressing concerns. Despite decades of research, nurses continue to struggle with high levels of stress and burnout, raising concerns about patient safety and error risk. One in 10 patients is estimated to experience an adverse patient event, with half being preventable, underscoring the need to understand the factors that affect stress among nurses and the subsequent risk of adverse patient events. This dissertation has the following aims: 1) to evaluate whether cognitive failure risk that could lead to an adverse patient event was associated with subjective measures of stress, …


Targeting Cancer With T Cells: Preclinical And In Silico Insights To Improve Immunotherapies, Nina Obertopp Mar 2026

Targeting Cancer With T Cells: Preclinical And In Silico Insights To Improve Immunotherapies, Nina Obertopp

USF Tampa Graduate Theses and Dissertations

T-cell–based immunotherapies are often constrained by limited trafficking and persistence in tumors and by functional exhaustion under chronic antigen exposure. This dissertation addresses these barriers through two distinct strategies: (i) preclinical studies testing how radiation therapy modulates tumor-infiltrating lymphocytes (TIL) therapy, and (ii) agent based modeling (ABM) to optimize treatment day treatment-free intervals (TFI) in T-cell engager (TCE) therapy. (i) In a human papillomavirus–positive (HPV⁺) head-and-neck cancer (HNC) model, a single local-dose of RT (8 Gy) delivered five days before tumor harvest increased the success and functional quality of ex vivo TIL outgrowth from tumor fragments, enriching polyfunctional CD8⁺ subsets. …


From Behind The Screen: Evaluating Teachers' Perceptions Of Online Professional Learning, Kathryn A. Rutledge Mar 2026

From Behind The Screen: Evaluating Teachers' Perceptions Of Online Professional Learning, Kathryn A. Rutledge

USF Tampa Graduate Theses and Dissertations

In recent years, online teacher professional learning has become a common format. However, in the large rural school district in central Florida where I work, the rising enrollment in online professional learning contrasted with declining completion rates among participants. My qualitative research study sought to explain why teachers’ perceptions of effective professional learning differ and why some teachers who enroll in asynchronous online professional learning courses fail to complete them. Using narrative inquiry, I collected data through qualitative questionnaires and semi-structured interviews with six teachers representing varied educational backgrounds. Analysis of the six participants’ interviews revealed five central themes: challenges …


Bullying And Discrimination Experiences Of Bhutanese College Students: Prevalence, Impacts, And Responses, Kezang Sherab, John Howard Mar 2026

Bullying And Discrimination Experiences Of Bhutanese College Students: Prevalence, Impacts, And Responses, Kezang Sherab, John Howard

Journal of Global Education and Research

Prior studies as well as media reports have identified bullying and discrimination amongst Bhutanese school and college students as a cause of concern, especially so for a nation with Gross National Happiness as its goal. Bullying and discrimination are associated with negative outcomes and can impact overall well-being. This quantitative study examined bullying and discrimination amongst college students (n = 16) in Bhutan through a self-administered online survey to a representative sample of 2043 (male = 40.8%; female = 55.8%). There was a non-significant decline from the 2016 survey in the reported prevalence of being bullied (from 45.2% to …


Does Abusive Supervision Undermine Creativity? A Buffering Role Of Absorptive Capacity, Sayma H. Shetu, Md. Sanaul Islam, Abdul G. Khan, Md. Shahed Mahmud Mar 2026

Does Abusive Supervision Undermine Creativity? A Buffering Role Of Absorptive Capacity, Sayma H. Shetu, Md. Sanaul Islam, Abdul G. Khan, Md. Shahed Mahmud

Journal of Global Business Insights

This study investigates the underlying processes of this relationship by examining knowledge hiding as a mediator and absorptive capacity as a moderator, within the context of the Conservation of Resources (COR) theory. 385 garment sector employees in Bangladesh were surveyed using a three-wave questionnaire, and the results were analyzed using partial least squares structural equation modeling. The study's findings demonstrate that abusive supervision negatively impacts employee creativity, in part by increasing knowledge hiding. Furthermore, higher absorptive capacity mitigates these adverse effects, reducing knowledge-hiding tendencies and enhancing creative performance. Theoretically, this study advances COR theory by identifying knowledge hiding as a …


Tourists’ Perceptions Of Autonomous Vehicles: A Qualitative Approach, Nese Yilmaz, Eda Ozgul-Katlav, Ozan Atsiz Mar 2026

Tourists’ Perceptions Of Autonomous Vehicles: A Qualitative Approach, Nese Yilmaz, Eda Ozgul-Katlav, Ozan Atsiz

Journal of Global Business Insights

This study examines travelers’ perceptions and attitudes toward autonomous vehicles (AVs) in tourism destinations. Adopting a qualitative approach, data were collected through semi-structured interviews with 20 travelers and analyzed using content analysis. Drawing on a cognitive–affective perspective, the findings reveal that travelers’ evaluations of AVs are shaped by both perceived advantages and disadvantages, which are closely linked to their perceptions and emotional responses. The results further indicate that cognitive (perceptions) and affective (feelings) responses are associated with behavioral intentions, including destination choice and willingness to pay for AV services. While many participants expressed positive expectations regarding convenience and novelty, concerns …


Undergraduate Health And Wellbeing Course And Study Away Program To Alaska, Lorraine S. Wallace, Steven J. Mousetes, Jennifer Bickley Mar 2026

Undergraduate Health And Wellbeing Course And Study Away Program To Alaska, Lorraine S. Wallace, Steven J. Mousetes, Jennifer Bickley

Journal of Global Education and Research

High-impact practices, such as diversity/global education, have been shown to positively contribute to student development, engagement, and success. As the US is both culturally and linguistically diverse, domestic study away programs can offer students impactful multicultural educational experiences without leaving the country. The purpose of this study was to describe the design of a health and well-being academic course and accompanying study away program to Alaska for undergraduates at The Ohio State University (OSU). The authors (1) describe the application of the backward design process in guiding ELOs development, (2) provide a sample of course assignments and student responses, and …


Globalizing The Engineering Job Market: Optimizing Engineering Career Expos To Serve International Students, Hailey A. Hesseltine Mar 2026

Globalizing The Engineering Job Market: Optimizing Engineering Career Expos To Serve International Students, Hailey A. Hesseltine

Journal of Global Education and Research

As the United States hosts approximately one million international students annually, it becomes imperative that on-campus resources are tailored to meet the needs of this diverse student population. One on-campus resource that is crucial to student success is career services, especially considering the aspirations of many international students to secure employment post-graduation. To address this, this study aimed to understand the satisfaction levels of international engineering students with an engineering career expo in Hawaiʻi. Utilizing a combination of surveys and interviews using a neo-racism framework, international students shared their experiences from the career expo through discussion and assessment. Information given …


Impact Of Femalized Kinesthetic Learning Model On African American Middle School Girls’ Career Interest, Mercy F. Fash, Andrea Ofori-Boadu Mar 2026

Impact Of Femalized Kinesthetic Learning Model On African American Middle School Girls’ Career Interest, Mercy F. Fash, Andrea Ofori-Boadu

Journal of Global Education and Research

Engaging African American middle school girls in out-of-school-time (OST) Architecture, Engineering, and Construction (AEC) programs can boost interest in male-dominated AEC careers. This study adopts Lent’s Social Cognitive Career Theory to evaluate the impact of an OST feminized AEC kinesthetic learning model (fAEC-KLM) on the career interests of this demographic. Through a qualitative research approach, fourteen (14) African American middle school girls from Guilford County, NC, completed pre- and post-interviews on how fAEC-KLM interactions influenced their AEC career interests. Inductive thematic analysis was carried out on transcribed interviews. Results show that effective components, such as lectures on AEC, bridge construction …


Advances In Culture, Tourism, And Hospitality Research: Volume 1, Cihan Cobanoglu, Hanqin Qiu, Jiaying Lyu, James H. W. Shen Feb 2026

Advances In Culture, Tourism, And Hospitality Research: Volume 1, Cihan Cobanoglu, Hanqin Qiu, Jiaying Lyu, James H. W. Shen

University of South Florida (USF) M3 Publishing

This is the first volume of the Advances in Culture, Tourism, and Hospitality Research Book Series.


Sensi-Train: Context-Aware Sensitive Content Detection Using Transformers And Large Language Models, Nazeefa Muzammil Feb 2026

Sensi-Train: Context-Aware Sensitive Content Detection Using Transformers And Large Language Models, Nazeefa Muzammil

USF Tampa Graduate Theses and Dissertations

The detection of sensitive content has become a critical task in natural language processing due to the widespread use of online platforms and the social harm caused by it. Even though current methods have advanced significantly, many of them mainly rely on surface-level text and neglect to take into consideration contextual and cultural elements that are crucial for correctly assessing sensitivity of any content to a particular individual. Due to this, implicit offense, sarcasm, and culturally grounded expressions frequently cause problems for such systems. To enable culturally grounded sensitive content detection, this thesis presents Sensi-Train, a context-rich dataset and modeling …


Geochemistry Of Crusts In Palaeontological Deposits, Taphonomic Settings In An Upper Pleistocene Cave Site (San Teodoro, Sicily), Gerlando Vita, Luca Sineo, Francesco Armetta, Maria Luisa Saladino Feb 2026

Geochemistry Of Crusts In Palaeontological Deposits, Taphonomic Settings In An Upper Pleistocene Cave Site (San Teodoro, Sicily), Gerlando Vita, Luca Sineo, Francesco Armetta, Maria Luisa Saladino

International Journal of Speleology

The mineralogical and geochemical composition of cave sediments provides crucial insights into past climatic and biological dynamics. This study investigates the authigenic crusts of San Teodoro Cave (Sicily), a key Upper Pleistocene palaeontological site, through a multi-analytical approach.The cave hosts two superimposed phosphatic crusts, separated by sterile sediment, reflecting shifts in depositional and post-depositional conditions. Our analyses reveal a complex mineral assemblage consisting of calcite, aragonite, and various phosphate phases. Calcite is the dominant phase in the older crust, whereas the presence of aragonite in the younger brown crust likely indicates periods of increased aridity, potentially corresponding to conditions during …


Inside The System, Outside The Circle: A Parent-Educator’S Journey Of Navigating Through Systemic Oppression While Advocating For Her Son With A Learning Disability, Sanora L. White Feb 2026

Inside The System, Outside The Circle: A Parent-Educator’S Journey Of Navigating Through Systemic Oppression While Advocating For Her Son With A Learning Disability, Sanora L. White

USF Tampa Graduate Theses and Dissertations

This autoethnographic study examines how I, as a Black parent-educator, navigated racialized and ableist educational systems while advocating for my child with a learning disability. Grounded in Disability Studies in Education, DisCrit, Critical Pedagogy, Universal Design for Learning, and Black Feminist Thought, the study explores how systemic inequities shape parent–teacher collaboration and how advocacy functions as relational care and resistance. The guiding research question asked how I experienced and navigated collaboration within racialized and ableist special education systems. Data sources included reflective journals, IEP documents, personal narratives, and dialogic conversations with critical friends. Using composite narratives and thematic analysis, the …


Lymphatic Malformations With Activating Kras Mutations Impair Lymphatic Valve Development Through Matrix Metalloproteinases, Diandra M. Mastrogiacomo Feb 2026

Lymphatic Malformations With Activating Kras Mutations Impair Lymphatic Valve Development Through Matrix Metalloproteinases, Diandra M. Mastrogiacomo

USF Tampa Graduate Theses and Dissertations

Lymphatic malformations (LMs) are lesions that can be due to inherited or somatic mutations, and they lead to a defective lymphatic vasculature. Activating KRAS mutations have been identified recently in LM patients with lymphedema, chylous ascites, or life-threatening chylothorax. In a LM mouse model, KRAS mutations are associated with a loss of lymphatic valves, which has been proposed to cause chylothorax via retrograde lymph flow into the pleural space. However, the mechanisms underlying the loss of lymphatic valves are unknown. To investigate the mechanisms leading to valve loss, we combined the lymphatic-specific and tamoxifen-inducible Flt4CreERT2 with Kras-loxP-stop-loxP-G12D (Kras+/G12D) mice and …