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Methods For Sampling Into An Atmospheric Pressure Inlet Mass Spectrometer, Theresa Evans-Nguyen, Linxia Song, Yi You

USF Patents

Provided herein are systems and methods for sampling analytes into an atmospheric pressure inlet mass spectrometer using ultrasonic nebulization-assisted atmospheric pressure chemical ionization. The systems can include a mass spectrometer having an input and an ultrasonic nebulizer chip. The ultrasonic nebulizer chip can be operatively coupled to the mass spectrometer, such that when the ultrasonic nebulizer chip nebulizes the analyte to provide a nebulized analyte, at least some of the nebulized analyte enters the input of the mass spectrometer.

Plasma Derived From Human Umbilical Cord Blood For The Treatment Of Neurodegenerative Disorders, Svitlana Garbuzova-Davis, Jared Carl Ehrhart, Paul R. Sanberg

USF Patents

A method of treating neurodegenerative diseases using hUCB plasma is presented herein. hUCB plasma attenuated the hyperactive response (Group III) and potentiated the normal response in Group I ALS patients, but did not alter that of the non-responders to PHA (Group II). The elevated activity of caspase 3/7 observed in the MNCs from ALS patients was significantly reduced by hUCB plasma treatment. The ability of hUCB plasma to modulate the mitogen cell response and reduce caspase activity suggest that the use of hUCB plasma alone, or with stem cells, may prove useful as a therapeutic in ALS patients. hUCB plasma …

Can The Effluent From Wastewater Stabilization Ponds Be Effectively Managed For Health And Nutrient Recovery?, Michael Glass

USF Tampa Graduate Theses and Dissertations

Wastewater stabilization ponds, such as facultative and maturation lagoons, have been shown to be effective processes to manage conventional water quality parameters found in domestic wastewater. The main goal of this thesis is to explore whether wastewater stabilization ponds can simultaneously reduce pathogens to safe levels while supporting agricultural activities through nutrient recovery. A large dataset for water quality of wastewater lagoon effluent in the U.S. was obtained at the state level and combined into regional southern and northern locations. Analysis of data showed there was significant difference in lagoon effluent concentration between northern and southern states for the water …

Beyond The Mask: Veterans Challenging The Strong Black Woman Trope In Their Journey To Heal In The South, Meya J. Hemphill-Hodges

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation is to emphasize notions of citizenship and accessibility as experienced by Black women Veterans. By expanding our worldview of Veteran identity, the ethnographic data demonstrate issues of displacement, alienation, and longing that contribute to the dissonance Black women Veterans rooted in the U.S. South may experience. To forge belonging through place-making, Black women Veterans may seek sisterhood by building sites of resistance in person or online. Findings are based on in-depth interviews with Black women Veterans and participant observation with community partners who service Veteran needs. Black women Veterans construct their identities often in oppressive …

New Ichnofossil Discovery In Mallorcan Caves: Linking Ancient And Moden Bat-Ectoparasites Coporolites., Antonio Merino Juncadella, Joan J. Fornós, Antoni Mulet, Joaquin Gines

International Journal of Speleology

Bat ectoparasitic ichnofossils can be useful to elucidate microbial mediation in mineralization processes of organic material, supplying valuable information on ancient parasitology and palaeoecology, among other topics. Rod-shaped features found in Cova des Pas de Vallgornera (Mallorca, Spain) are described for the first time. They are related to an ancient bat colony inhabiting the cave in the Early Pleistocene. These micro-morphologies are cylindrical in shape with sizes of a few hundred microns in length and less than one hundred microns in diameter. They are composed by a mash of microscopic calcite particles of different sizes and shapes, lying on deposits …

Preparing Children For Kindergarten Readiness: Early Childhood Teachers’ Goals And Practices, Julie Chappell

USF Tampa Graduate Theses and Dissertations

Literature in the field of early childhood education suggests that standardization and the accountability movement in the United States has changed the way that teachers approach kindergarten, resulting in less play and more teacher-directed activities. This pedagogical shift causes challenges for early childhood educators striving to promote developmentally appropriate, play-based and child-centered approaches to learning for young children while preparing children for the expectations of kindergarten. The purpose of this qualitative study was to gain an understanding of prekindergarten teachers’ views about their role in preparing children for kindergarten. The study was guided by two research questions: How do prekindergarten …

Six-Year College Graduation Status For High School Students Participating In An Upward Bound Program, Raymond Upward Cabrera

USF Tampa Graduate Theses and Dissertations

This study examines the impact of high school academic indicators and participation in the federally funded Upward Bound program on college graduation rates for first-generation, low-income students. Specifically the extent to which participation in the federally funded TRIO Upward Bound program and related factors—high school GPA, course rigor, program involvement, gender, race, and college type—predict 6-year college graduation rates for first-generation, low-income students. Using stepwise logistic regression analysis on data of 163 students in an Upward Bound program in the Southeast, the results show that students with higher GPAs, active engagement in the program, and females were significantly more likely …

Life History Of Men Who Experienced Childhood Maternal Absence, Renice Obure

USF Tampa Graduate Theses and Dissertations

This dissertation project explored the impact of childhood maternal absence on the life history outcomes of adult men. An integrated theoretical model of developmental-evolutionary framework was developed to guide research design and conduct. This model combined sex-differentiated life history framework and fathering resource theory. The study consisted of three components: a scoping review of 29 relevant studies conducted in the United States between 1990-2023, a quantitative analysis of the National Longitudinal Study of Adolescent and Adult Health (Add Health) waves I and IV (n=6932), and a qualitative analysis of in-depth interviews with a sample of 22 fathers who experienced childhood …

Improving College Students’ Attention Retention With A Brain-Controlled Drone Simulation, Ji Won Bae

USF Tampa Graduate Theses and Dissertations

In contemporary society, individuals are continuously exposed to a plethora of stimuli, which can precipitate distractions and impede cognitive performance in tasks such as professional work and academic studies. This investigation proposes an innovative approach aimed at enhancing attentional focus through the utilization of Brain-Computer Interfaces (BCI). BCIs represent advanced methodologies for deciphering neural activity patterns. Specifically,electroencephalography (EEG), a technique for monitoring the brain's electrical signals, serves as the foundation for BCI applications. EEG analyses reveal distinctive wave patterns indicative of states of concentration, vigilance, and cognitive engagement. Consequently, BCIs hold promise for the real-time assessment of users' attentional states. …

Spatiotemporal Dynamics Of Coastal Nekton Metacommunities In The Western Atlantic, Jonathan A. Peake

USF Tampa Graduate Theses and Dissertations

The processes of diversification, selection, dispersal, and stochastic drift can combine in many ways to assemble ecological communities. A key focus in ecology is to describe and quantify the relative influences of these processes for different systems and explain the mechanisms by which these processes produce patterns of community structure. To accomplish this goal, ecologists have developed several frameworks that seek to integrate processes that occur locally andregionally. The metacommunity concept treats local communities as pieces of a broader landscape that are connected through dispersal of their constituent organisms. Empirical studies have generally lagged behind the development of metacommunity theory. …

Cactus Infused Beads For Off-Flavor Mitigation In Aquaculture Systems, Rama Mahita Kambhampati

USF Tampa Graduate Theses and Dissertations

Aquaculture has become a vital component of global food production, now providing 50% of the world's fish supply. However, the industry encounters major challenges due to off-flavor compounds like Geosmin and 2-Methylisoborneol (MIB), which give fish an unpleasant earthy or musty smell. These compounds are detectable by humans at extremely low levels 0.7 parts per billion (ppb) for MIB and ten parts per trillion (ppt) for Geosmin—making them a persistent problem. Their presence not only reduces the sensory quality of aquaculture products but also results in significant economic losses, with U.S. catfish production incurring an additional $15–$23 million annually due …

Phosphoglycerate Dehydrogenase Regulated By The Mitogen-Activated Protein Kinase Pathway Is An Actionable Vulnerability In Cutaneous Melanoma, Neel Jasani

USF Tampa Graduate Theses and Dissertations

Metabolic alterations play a critical role in shaping tumor growth and development. Many metabolic enzymes are shown to play an important role in different aspects of tumor development, growth, and metastasis. One such enzyme that we are investigating in this project is the Phosphoglycerate Dehydrogenase (PHGDH). The PHGDH gene, encoding the rate-limiting enzyme of the serine synthesis pathway, is located at chromosome locus 1p12 and frequently amplified in melanoma. PHGDH is required for the proliferation of melanoma cells with chromosome 1p12 amplification. However, the role of PHGDH in chromosome 1p12 non-amplified melanoma is poorly understood. In this project, we sought …

Analyzing And Extending Machine Learning Frameworks On High Risk Domains, Chengbin Hu

USF Tampa Graduate Theses and Dissertations

Machine learning (ML) has become a transformative force in high-risk domains such as genomics and cybersecurity, where accurate predictions and robust defenses are essential. This dissertation advances ML frameworks in these areas by developing methods to enhance predictive power in health applications and assess vulnerabilities in machine learning systems.

In the genomics field, the work addresses challenges in Non-Invasive Prenatal Testing (NIPT) of monogenic disorders by proposing a deep learning model that reconstructs the fetal genome using maternal plasma cell-free DNA (cfDNA) and parental whole-genome sequencing (WGS) data. This model achieves high accuracy in single nucleotide variation (SNV) prediction, surpassing …

Telu Activation Function For Fast And Stable Deep Learning, Alfredo Fernandez

USF Tampa Graduate Theses and Dissertations

We propose the Hyperbolic Tangent Exponential Linear Unit (TeLU), a neural network hidden activation function defined as $TeLU(x)=x \cdot tanh(e^x)$. TeLU’s design is grounded in the core principles of key activation functions, achieving strong convergence by closely approximating the identity function in its active region while effectively mitigating the vanishing gradient problem in its saturating region. Its simple formulation enhances computational efficiency, leading to improvements in scalability and convergence speed. Unlike many modern activation functions, TeLU seamlessly combines the simplicity and effectiveness of ReLU with the smoothness and analytic properties essential for learning stability in deep neural networks. TeLU’s ability …

Characterizing The Temporal And Nearshore To Offshore Spatial Scale Of Phytoplankton Assemblages Within The Florida Keys National Marine Sanctuary, Sebastian P. Di Geronimo

USF Tampa Graduate Theses and Dissertations

This study investigates the spatial and temporal dynamics of phytoplankton communities within the Florida Keys National Marine Sanctuary (FKNMS) from 2016 to 2021. Phytoplankton biomass and community composition were analyzed across four seasons and from both Nearshore (within 2 km of shore) and Offshore (8 to 10 km from shore) locations along the Florida Keys from Miami to the Western Sambo Ecological Reserve. High-performance liquid chromatography (HPLC) quantified chlorophyll-a (tchla) as a proxy for bulk biomass, and other pigments. CHEMTAX, a chemotaxonomic identification method, was employed to estimate phytoplankton community composition. Our results revealed significant seasonal variability in bulk biomass, …

Satellite-Based Mapping Of Seagrass Habitats And Coastal Wetlands In The Us Gulf Of Mexico And Florida: A Remote Sensing Approach For Environmental Conservation And Management, Luis Lizcano-Sandoval

USF Tampa Graduate Theses and Dissertations

Coastal wetlands and seagrass meadows provide multiple services to nature and human society, but their spatial and temporal changes are often not well characterized. They are habitats that are threatened by natural and human causes such as changes in water quality, urban development, pollution, and climate change, among others. Coastal ecosystems rely on water quality and sea level variability for survival, and changes in those variables and their potential effects are of concern to many coastal communities. Satellite remote sensing is a powerful tool that has been used for mapping ecosystem distribution on land and in the ocean. Satellite sensors …

On The Role Of Prediction In Streaming Hierarchical Learning, Ramy Mounir

USF Tampa Graduate Theses and Dissertations

In today's world, AI systems need to make sense of large amounts of data as it unfolds in real-time, whether it's a video from surveillance and monitoring cameras, streams of egocentric footage, or sequences in other domains such as text or audio. The ability to break these continuous data streams into meaningful events, discover nested structures, and predict what might happen next at different levels of abstraction is crucial for applications ranging from passive surveillance systems to sensory-motor autonomous learning. However, most existing models rely heavily on large, annotated datasets with fixed data distributions and offline epoch-based training, which makes …

Zooplankton Abundance, Composition, And Diversity And Associated Environmental Factors Across The Gulf Of Mexico And Along The Northwestern Coast Of Cuba, Shaniqua J. Gladney

USF Tampa Graduate Theses and Dissertations

My research focused on characterizing the zooplankton community of two regions in the Gulf of Mexico (GOM) along two transects, one across the middle of the GOM from Tuxpan, Mexico, to St. Petersburg, FL, and one along the northwestern coast of Cuba. Zooplankton bongo samples and associated environmental and physical processes were examined. These data were collected during two Center for Integrated Modeling and Analysis of Gulf Ecosystems (C-IMAGE) expeditions aboard the R/V Weatherbird II, during September 2015 (GOM) and May 2017 (Cuba). A Zooscan imaging system was used to identify and enumerate zooplankton taxa. Then zooplankton abundance, composition, and …

Patient Reported Outcomes Amidst Isolated And Concomitant Chronic Cardiopulmonary Illnesses, Walter B. Wills

USF Tampa Graduate Theses and Dissertations

As the prevalence of many chronic cardiopulmonary illnesses are expected to rise within the United States through 2030, there is a growing emphasis on investigating the patient-reported outcomes relevant to the morbidity and social burden of these diseases. Thus, the overall aim of this dissertation is to examine these outcomes among those that live with chronic obstructive pulmonary disease and cardiovascular diseases (namely, heart failure). Specifically, the works in this dissertation aimed to compare how individuals report their health-related quality of life when living with and without cardiovascular disease across the spectrum of airflow limitation demonstrated within chronic obstructive pulmonary …

A Phenotypic Characterization Of Staphylococcus Aureus Bacteremia Clinical Isolates, Sara Dj Daniels

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a human pathogen of serious concern because of its ability to cause serious and wide-ranging disease. Of particular concern is the ability of S. aureus to cause bacteremia (SAB), allowing the bacteria to disseminate and spread to various organs. This study addressed this issue through the phenotypic characterization of two methicillin-resistant S. aureus (MRSA) clinical isolates taken from patients with sustained complicated bacteremia at Tampa General Hospital, referred to as SA1097 and SA1098. A battery of phenotypic tests was conducted on these strains, including a growth curve, hemolysis and proteolysis screenings, cytotoxicity assays using human neutrophils, blood …

Selective Pericyte Recruitment To Ec-Lined Tubes, Vessel Assembly Enhancement Through Pericyte Timp-3 Induction, And Pericyte Tbx2 In Capillary Formation And Maturation, Ksenia Marilyn Yrigoin Kaluguina

USF Tampa Graduate Theses and Dissertations

Capillaries, the smallest blood vascular networks, are formed by narrow and elongated endothelial cell (EC)-lined tubes with associated pericytes anchored to the abluminal tube surface. Our laboratory demonstrated that dynamic interactions between ECs and pericytes during capillary tube network assembly are required for basement membrane matrix deposition and maturation. We have also shown that key EC-derived factors promote pericyte recruitment, proliferation, and pericyte-induced basement membrane formation during capillary assembly. In the first section, we aim to identify if vascular smooth muscle cells (VSMCs) respond to the same factors and have the same invasive ability as pericytes since they are both …

Rictor, A Mtorc2 Protein, Regulates Lymphatic Valve Formation And Maintenance Through The Akt-Foxo1 Signaling Axis, Richa Banerjee

USF Tampa Graduate Theses and Dissertations

The lymphatic vasculature maintains tissue fluid homeostasis by absorbing interstitial fluid to form lymph and transporting it back to the bloodstream. Collecting lymphatic vessels have lymphatic valves (LVs) that prevent the retrograde flow of lymph generated by hydrostatic pressure from the blood vasculature. Backflow and accumulation of lymph leads to a lymphatic pathology called lymphedema. This implicates the involvement of defective LVs in the pathogenesis of lymphedema and warrants the study of lymphatic valve development and maintenance. The LVs are formed through a process called mechanotransduction which signals through the intracellular PI3K-AKT pathway in lymphatic endothelial cells. However, the activation …

The Attitudes, Perceptions, And Motivations Of Student-Athletes In Intermediate Algebra, Alexandra Green

USF Tampa Graduate Theses and Dissertations

This research delves into the attitudes, perceptions, and motivations of student-athletes enrolled in Intermediate Algebra courses. It focuses on the distinct challenges they encounter in juggling academic and athletic responsibilities at a southeastern university in the United States. Employing a mixed-methods approach, the study gathered quantitative data through surveys to assess students' attitudes, perceptions, and motivations toward learning mathematics, as well as to determine the influence of their instructors on these factors. The qualitative segment provided deeper insights into their personal experiences, shedding light on their prior encounters with mathematics, their most memorable moments during the current course, and their …

Essays On Information Technology In Healthcare, Gleb Zavadskiy

USF Tampa Graduate Theses and Dissertations

Information technologies (IT) and information systems (IS) have profound significance across various sectors of society, including healthcare, business, education, government, and beyond. First, IT facilitates instant communication globally through email, messaging apps, video conferencing, and social media, revolutionizing how individuals and organizations interact, collaborate, and share information (Hacker et al. 2020; Tang and Hew 2020).

Secondly, the Internet and digital libraries provide worldwide access to vast amounts of information, what makes knowledge and education available to everyone, empowering individuals to learn and stay informed on diverse topics (Haleem et al. 2022). Another aspect of IT systems in various industries is …

The Pilling Process: Significance Quest, Self-Verification, And Fusion In Online Incel Forums, Gregory J. Rousis

USF Tampa Graduate Theses and Dissertations

Incels (short for “involuntary celibates) are an online community of men who blame women for their reputed sexual and romantic failures. The misogyny of their worldview, called the blackpill, has inspired multiple instances of real-world violence against women. Alarmingly, we know little about the temporal processes whereby an incel goes from joining an online community to becoming deeply aligned (i.e., “fused”) with the group. This project sought to address this by scraping incels’ most popular web forum and using text analysis to identify psychological mechanisms that could be responsible for incels’ fusion. The resulting dataset consisted of 1,097,841 unique discussion …

Transition Aged Youth Substance Use At Electric Dance Music Events In The Tampa Bay Area: A Qualitative Study Of Awareness And Prevention, Danielle Vinciguerra

USF Tampa Graduate Theses and Dissertations

The study investigates substance use among Transitional Aged Youth (TAY), particularly focusing on Electronic Dance Music (EDM) events in the Tampa Bay area. TAY, defined as individuals aged 18-29, experience significant psychological, biological, and developmental changes, which can lead to increased vulnerability to substance use. The study examines the prevalence and patterns of substance use, specifically ketamine and cocaine, among TAY at EDM events. Exploratory qualitative data collection method was utilized for the study. Applying Social Learning Theory as a framework, the research explores the beliefs, attitudes, experiences, and community influences that contribute to substance use within this demographic. Data …

Unravelling The Impact Of Blood Metabolites, And Lifestyle Factors On Periodontal Disease Using Mendelian Randomization, Rhea Charles

USF Tampa Graduate Theses and Dissertations

Periodontal disease remains a global public health concern. Despite the availability of preventive and therapeutic strategies, the multifactorial nature of periodontitis complicates its understanding and management. Periodontal disease is associated with an increased risk of chronic conditions, including ischemic heart disease (IHD), gestational hypertension, respiratory diseases such as COPD and bronchitis, and various cancers, including kidney and pancreatic cancers. Moreover, individuals with diabetes, autoimmune diseases like lupus and Crohn’s disease, and osteoporosis are more likely to develop periodontitis. The ambiguity in the direction of causality between periodontal disease and its associated conditions poses challenges for effective treatment. Mendelian randomization offers …

Integrating Multiple Biodiversity Metrics To Assess Diatom Diversity And Diatom/Macrophyte Host Specificity In Four Florida Benchmark Lakes, Alana Jade Bronk

USF Tampa Graduate Theses and Dissertations

Biodiversity is declining across all natural environments, however, freshwater systems are thought to be among the most threatened. Quantifying and understanding trends in freshwater biodiversity has become more urgent as rapid biodiversity loss continues. To provide a robust ecological assessment, more than one bioindicator should be used, along with multiple biodiversity metrics. Macrophytes and diatoms are important components of aquatic systems, especially in shallow lakes. The relationship between macrophytes and epiphytic diatoms, though, is not clearly understood, and has not been well-studied, particularly in Florida, which has the largest abundance of natural freshwater lakes in the southeastern U.S This study …

Back Matter, Maryland Birdlife

Maryland Birdlife

No abstract provided.

Tongue-In-Cheek Dictionary Of Birding Terms, Eirik A. T. Blom

Maryland Birdlife

No abstract provided.