Digital Commons Network^™

Impact Of Prolonged Isolation On Adolescents With Drug-Susceptible Tuberculosis In Lima, Peru: A Qualitative Study, Victoria Elena Rapoport, Elmer Altamirano, Liz Senador, Milagros Wong, Catherine B. Beckhorn, Julia Coit, Stephanie D. Roche, Leonid Lecca, Jerome T. Galea, Silvia S. Chiang

Social Work Faculty Publications

Objectives Patients with tuberculosis (TB) generally are instructed to isolate at the beginning of treatment in order to prevent disease transmission. The duration of isolation varies and may be prolonged (ie, lasting 1 month or more). Few studies have examined the impact of isolation during TB treatment on adolescents, who may be more vulnerable to its negative effects.

Methods This study took place from 2018 through 2019 in Lima, Peru, where the Ministry of Health mandates the exclusion of patients with TB from educational institutions for at least 2 months. Using semi-structured guides, we conducted individual in-depth interviews with adolescents …

Data In Context: How Data Fit Into The Scholarly Conversation, Theresa Burress

USF St. Petersburg Campus Faculty Publications

No abstract provided.

An Ayurgenomics Approach: Prakriti-Based Drug Discovery And Development For Personalized Care, Zoufang Huang, Vivek P. Chavda, Rajashri Bezbaruah, Vladimir N. Uversky, Sucharitha Palagati, Aayushi B. Patel, Zhe-Sheng Chen

Molecular Medicine Faculty Publications

Originating in ancient India, Ayurveda is an alternative medicinal approach that provides substantial evidence for a theoretical-level analysis of all aspects of life. Unlike modern medicine, Ayurveda is based upon tridoshas (Vata, pitta, and Kapha) and Prakriti. On the other hand, the research of all the genes involved at the proteomics, metabolomics, and transcriptome levels are referred to as genomics. Geoclimatic regions (deshanupatini), familial characteristics (kulanupatini), and ethnicity (jatiprasakta) have all been shown to affect phenotypic variability. The combination of genomics with Ayurveda known as ayurgenomics provided new insights into tridosha that may pave the way for precision medicine (personalized …

Arrow Of Time, Entropy, And Protein Folding: Holistic View On Biochirality, Victor V. Dyakin, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Chirality is a universal phenomenon, embracing the space–time domains of non-organic and organic nature. The biological time arrow, evident in the aging of proteins and organisms, should be linked to the prevalent biomolecular chirality. This hypothesis drives our exploration of protein aging, in relation to the biological aging of an organism. Recent advances in the chirality discrimination methods and theoretical considerations of the non-equilibrium thermodynamics clarify the fundamental issues, concerning the biphasic, alternative, and stepwise changes in the conformational entropy associated with protein folding. Living cells represent open, non-equilibrium, self-organizing, and dissipative systems. The non-equilibrium thermodynamics of cell biology are …

Fundamental Clock Of Biological Aging: Convergence Of Molecular, Neurodegenerative, Cognitive And Psychiatric Pathways: Non-Equilibrium Thermodynamics Meet Psychology, Victor V. Dyakin, Nuka V. Dyakina-Fagnano, Laura B. Mcintire, Vladimir N. Uversky

Molecular Medicine Faculty Publications

In humans, age-associated degrading changes, widely observed in molecular and cellular processes underly the time-dependent decline in spatial navigation, time perception, cognitive and psychological abilities, and memory. Cross-talk of biological, cognitive, and psychological clocks provides an integrative contribution to healthy and advanced aging. At the molecular level, genome, proteome, and lipidome instability are widely recognized as the primary causal factors in aging. We narrow attention to the roles of protein aging linked to prevalent amino acids chirality, enzymatic and spontaneous (non-enzymatic) post-translational modifications (PTMs SP), and non-equilibrium phase transitions. The homochirality of protein synthesis, resulting in the steady-state non-equilibrium condition …

Are The Functions Of Milk Exosomes Restricted To Their Protein Cargoes?, Elrashdy M. Redwan, Vladimir N. Uversky

Molecular Medicine Faculty Publications

No abstract provided.

Status, Change, And Futures Of Zooplankton In The Southern Ocean, Nadine M. Johnston, Eugene J. Murphy, Angus Atkinson, Andrew J. Constable, Cédric Cotté, Martin Cox, Kendra L. Daly, Ryan Driscoll, Hauke Flores, Svenja Halfter, Natasha Henschke, Simeon L. Hill, Juan Höfer, Brian P. Hunt, So Kawaguchi, Dhugal Lindsay, Cecilia Liszka, Valerie Loeb, Clara Manno, Bettina Meyer, Evgeny A. Pakhomov, Matthew H. Pinkerton, Christian S. Reiss, Kate Richerson, Walker O. Jr., Deborah K. Steinberg, Kerrie M. Swadling, Geraint A. Tarling, Sally E. Thorpe, Devi Veytia, Peter Ward, Christine K. Weldrick, Guang Yang

Marine Science Faculty Publications

In the Southern Ocean, several zooplankton taxonomic groups, euphausiids, copepods, salps and pteropods, are notable because of their biomass and abundance and their roles in maintaining food webs and ecosystem structure and function, including the provision of globally important ecosystem services. These groups are consumers of microbes, primary and secondary producers, and are prey for fishes, cephalopods, seabirds, and marine mammals. In providing the link between microbes, primary production, and higher trophic levels these taxa influence energy flows, biological production and biomass, biogeochemical cycles, carbon flux and food web interactions thereby modulating the structure and functioning of ecosystems. Additionally, Antarctic …

Bioactive Peptides: Synthesis, Sources, Applications, And Proposed Mechanisms Of Action, Mohsen Akbarian, Ali Khani, Sara Eghbalpour, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Bioactive peptides are a group of biological molecules that are normally buried in the structure of parent proteins and become active after the cleavage of the proteins. Another group of peptides is actively produced and found in many microorganisms and the body of organisms. Today, many groups of bioactive peptides have been marketed chemically or recombinantly. This article reviews the various production methods and sources of these important/ubiquitous and useful biomolecules. Their applications, such as antimicrobial, antihypertensive, antioxidant activities, blood-lipid-lowering effect, opioid role, antiobesity, ability to bind minerals, antidiabetic, and antiaging effects, will be explored. The types of pathways proposed …

Higher Native Peruvian Genetic Ancestry Proportion Is Associated With Tuberculosis Progression Risk, Samira Asgari, Yang Luo, Chuan-Chin Huang, Zibiao Zhang, Roger Calderon, Carmen Contreras, Jerome T. Galea, Leonid Lecca, David Jones, D. Branch Moody, Megan B. Murray, Soumya Raychaudhuri

Social Work Faculty Publications

No abstract provided.

Contributions Of Agroforestry Systems To Food Provisioning Of Peasant Households: Conflicts And Synergies In Chiapas, Mexico, Lorena Soto-Pinto, Sandra Escobar Colmenares, Marina Benítez Kanter, Angelita López Cruz, Erin Estrada Lugo, Balente Herrera Hernández, Estelí Jiménez-Soto

School of Geosciences Faculty and Staff Publications

Traditional agroforestry systems are widely recognized for their contributions to provisioning, support, regulation, and cultural services. However, because of the advancement of industrial agriculture and a corporative food system, peasants' food systems are rapidly undergoing transformation. We identify the contributions of four types of agroforestry systems (AFS)—shade cocoa agroforest, shade coffee agroforest, milpas and homegardens—to food provisioning in peasant families and discuss conflicts between traditional food systems and the contemporary industrial model of production and consumption confronted by peasants and semi-proletarian migrants. We carried out research in 17 peasant communities in Chiapas, Mexico, and conducted 97 semi-structured interviews and agroecological …

Marketing Tourism And Hotel Management Schools In The Context Of Higher Education Globalization And Student Mobility Through Improved Curriculum And Industrial Training Offerings, Ayse Bas Collins, Aysegul Gunduz Songur, Seden Dogan

Journal of Global Education and Research

Recent research from international statistics indicate an important flow of student mobility all over the world, creating a need to provide comprehensive information regarding educational institutions involved in the process. This study examines and compares tourism and hotel management (THM) programs at different levels, including the nature of programs offered, their curriculum, and internship components. A particular focus was given to the industrial training requirement as a core component of THM programs. Results showed both similarities and differences worldwide within the framework of the institutions compared. The findings may help all stakeholders in the education systems, including professionals, educators, students, …

A Biocultural Analysis Of The Impacts Of Interactions Between West Africans And Europeans During The Trans-Atlantic Trade At Elmina, Ghana, Heidi Ellen Miller

USF Tampa Graduate Theses and Dissertations

This project utilizes a biocultural approach to assess the demographics and health of the West African population from Elmina, Ghana. Elmina, selected by the Portuguese in 1482 as the site of the first European trade fort in sub-Saharan Africa, grew from a small coastal fishing village to a large settlement over the course of more than 400 years of trade and cultural entanglement. Taken over by the Dutch and then ceded to the British, the people of Elmina navigated significant cultural changes, changes and experiences that can be detected in their skeletal remains. Bioarchaeological research concerned with the effects of …

Learning Connections Inventory© Validation Using A Physician Assistant Training Program, Roberta Worsham

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to validate the Learning Connections Inventory for use with the military Physician Assistant students at the Interservice Physician Assistant Program at Fort Sam Houston, TX. The 61 participants in this study completed both a demographic survey and the Learning Connections Inventory (LCI©). The results of the demographic questionnaire depicted the characteristics of the participants and the LCI© described the learning patterns of each participant.

Research Questions one, confirmation of the current LCI© model, and two, the instructor assessment and the current model agreement, were answered using confirmatory factor analysis (CFA) and construct validity. The …

Effectiveness Of An Early Literacy Intervention For Increasing Teen Parents' Child-Directed Speech And Conversational Turns, Deborah H. Christie

USF Tampa Graduate Theses and Dissertations

Children’s language ability upon entry to kindergarten is a powerful predictor of reading achievement throughout elementary school; yet disparities in children’s language growth have been detected as early as 18 months of age. These disparities have been linked to the quantity and quality of speech provided to children as they are learning to talk. The current study employed a single-case multiple-baseline across participants experimental design to evaluate the effectiveness of an early literacy intervention to increase teen parents’ child-directed speech and conversational turns. The intervention was delivered one-on-one via videoconferencing by a teen parent peer coach. Participants included teen parents …

The Influence Of Intrinsic Motivational Forces On Consumers’ Product Purchase Intentions, Sumera Syed, Syed R. H. Shah, Fauziah S. Ahmad

Journal of Mediterranean Tourism Research

Food is perceived as the top most surviving need of humans since their existence. With the passage of time, this need has been further revolutionized with added requirements of safety and hygienic factors. Halal food having both of these factors, has gained popularity among both Muslims and non-Muslims consumers. Despite of ever increasing trend of Halal food consumption, the research to uncap this amazingly growing sector is still in its infancy stages. Interestingly, the role of intrinsic motivation in shaping up intention of the consumers for Halal food purchase is still not well explored. This study has addressed this untapped …

Learning, Planning For, And Reflecting On Teaching Mathematics For Social Justice: A Case Study Investigation Of A High School Mathematics Methods Course, Queshonda J. Kudaisi

USF Tampa Graduate Theses and Dissertations

While much is known about learning to teach for social justice in other content areas, less is known about how mathematics education students learn to teach mathematics for social justice, specifically at the high school level. Hence, this mixed-methods convergent case study addresses this gap in the research literature by examining and describing the experiences of mathematics education students learning to teach mathematics for social justice within the context of a high school mathematics methods course at a large research university in the southeastern United States. The data sources accessed for this study include a survey measuring beliefs about teaching …

Microbial Dark Matter: Culturing The Uncultured In Search Of Novel Chemotaxonomy, Sarah J. Kennedy

USF Tampa Graduate Theses and Dissertations

Although <1% of environmental isolates can be cultured, insights into the culturable fraction refine future techniques to unlock the cryptic biodiversity. This cryptic biodiversity is omnipresent within culture-independent surveys of environmental bacterial populations. Subsequently, these elusive organisms are termed “microbial dark matter” due to the intrigue surrounding their potential taxonomic and biosynthetic impacts. To this end of investigating bacterial-specific dark matter, we employed high-throughput culturing techniques to examine Gulf of Mexico sediments and sponges for chemotaxonomically important bacteria. Through stepwise implementation of permissive and selective recovery techniques, we succeeded in recovering over 150 unique bacterial isolates. We observed several isolates that pose as chemotaxonomically interesting, including several strains that belong to critical bioremediation taxa. In addition to these biotechnological-amenable isolates, we have also succeeded in culturing taxa historically attributed with antibacterial discoveries. One such isolate was a previously sponge-associated organism belonging to the rarely cultured Verrucosispora genus. Through extensive comparative genomics, we identified it as a novel species and subsequently named it Verrucosispora sioxanthis sp. nov. with the published genome serving as a resource for continued microbial dark matter investigations. Following continued passaging of the organism, we observed improved growth, biomass, and liquid growth dispersal within each increasingly sub-passaged generation of the isolate. As such, the laboratory attenuation, or domestication, of the organism warranted in-depth whole genomic analyses to generate insight into the increased laboratory sustainability. Through phenotypically-guided bioinformatics, we determined that the domestication was mediated through genome-wide selective pseudo- or non-pseudogenization of genes. As the previously sponge-associated organism evolved with the selection pressures of the static laboratory, we demonstrate that isolate effectively underwent genomic atrophy to resolve into a largely stable, sustainable laboratory-domesticated strain. Collectively, our chemotaxonomic discovery pipeline expands on the culturable bacterial biodiversity and ultimately provides a previously uncultured model organism for initial forays into resolving the microbial dark matter knowledge gap.

Sanitation Marketing In Uganda: The Impact Of Gender On Participation And Outcomes, Elizabeth Vicario

USF Tampa Graduate Theses and Dissertations

Uganda aims to reach universal access to basic sanitation under Sustainable Development Goal #6, but achieving this target by 2030 would require doubling the annual rate of progress. Women are disproportionately affected by poor sanitation due to their health needs and risk of facing violence, but they have historically been excluded from sanitation planning. Low household demand and poor service delivery have stalled progress in Uganda. Market-based sanitation (MBS) is a novel method of developing a sanitation market through stimulating demand and supporting a competitive atmosphere for product and service providers. This research studies the sanitation marketing activities implemented by …

Evaluating Daily Systemic Betaine Delivery In Alleviating Dry-Amd Pathophysiology, Dawin Khiev

USF Tampa Graduate Theses and Dissertations

MnSod (Sod2) deletion in the retinal pigment epithelium (RPE) of Sod2flox/floxVMD2-cre mice leads to oxidative stress-induced apoptosis of the RPE and photoreceptor cells that show salient features of dry-AMD. The purpose of this study was to investigate the potential antioxidant effect of betaine in protecting retinal health in Sod2flox/floxVMD2-cre mice. Betaine low dose (50mg/Kg Body Weight) and high dose (500mg/kg Body Weight, n=9) were administered intraperitoneally to 8-weeks old male and female Sod2flox/floxVMD2-cre mice daily for four months, while the control group (Sod2flox/floxVMD2-cre mice; n=9) received saline. We used spectral-domain optical coherence tomography (SD-OCT), retinal outer nuclear layer (ONL) thickness …

Seasonality In Competence To Transmit West Nile Virus For A Widespread Reservoir, Kyle L. Koller

USF Tampa Graduate Theses and Dissertations

All organisms face a central conflict; a limited supply of resources must beoptimally allocated to competing biological functions. Utilizing robust immune defenses can be energetically costly;, thus, immune function is often diminished when other processes, such as reproduction, molt, or migration demand energy. As wild animals residing in temporally dynamic environments face seasonal patterns in resource availability, infectious disease risk, and environmental suitability, many species have evolved endogenous biological rhythms so that the performance of reproduction, molt, or migration and the associated trade-off with immune function be completed when doing so best promotes fitness. In turn, this demand to optimize …

Computer Simulation For Continuous Centrifugation Of High-Density Cell Cultures, Hironori Tomizawa

USF Tampa Graduate Theses and Dissertations

Perfusion bioreactors are used for cell production for cell therapies. The system uses cell separation devices such as centrifuge to facilitate cell growth by separating cells and spent media and allows constant media replacement. However, current cell separation technologies can increase shear stress, are generally not continuous processes, and are not as efficient as desired. These limit the amount of media replaced per unit time. Therefore, there is a need for a new system or a method that operates continuously to minimize cell damage, yield high cell concentration, and exchange media effectively. A set of research questions was formulated: (1) …

The Weekly Challenger, October 14, 2021, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The October 14, 2021, issue of The Weekly Challenger.

Who To Choose? Rating Broker Best Practices In The Medicare Advantage Industry, Darwin R. Hale

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to help navigate the U.S. Healthcare system in a way that maximizes value and minimizes risk to Medicare Beneficiaries. We do this through the creation of a tool that will aid in the selection of an independent health broker. Brokers are the trusted resource who have the responsibility and expertise to optimize client value through needs analysis, education, and expectations setting. Beneficiaries need help with life impacting health and wellness decisions and ongoing support across the continuum of care. The qualitative research included interviews of industry experts with combined Medicare experience of more than …

Crow's Nest, October 11, 2021, Crow's Nest Staff

Crow's Nest

The October 11, 2021, issue of the Crow's Nest.

Mitigation Strategies For Central Nervous System Oxygen Toxicity In Rodents: Ketone Metabolic Therapy And Mitochondrial Antioxidant Therapy, Christopher Manuel Hinojo

USF Tampa Graduate Theses and Dissertations

Hyperbaric oxygen (HBO2) is a frequently encountered condition in undersea medicine and hyperbaric oxygen therapy (HBOT). The risk of CNS oxygen toxicity (CNS-OT) seizures limits its use in hyperbaric medicine and limits bottom time for diving operations. In this study, we sought to understand the role of reactive oxygen species (ROS) in two mitigation strategies for CNS-OT; ketone metabolic therapy (KMT), which is known to delay onset of CNS-OT seizures, and mitochondria targeted antioxidant therapy (MitoTAT), which has never been tested under HBO2 conditions. We specifically focused on superoxide anions, one of the early reduction products of molecular oxygen, and …

Pinpointing Patterns Of Violence: A Comparative Genocide Studies Approach To Violence Escalation In The Ukrainian Holodomor, Kristina Hook

Genocide Studies and Prevention: An International Journal

This article utilizes the case study of the 1930s Ukrainian Holodomor, an artificially induced famine under Joseph Stalin, to advance comparative genocide studies debates regarding the nature, onset, and prevention of large-scale violence. Fieldwide debates question how to 1) distinguish genocide from other forms of large-scale violence and 2) trace genocides as unfolding processes, rather than crescendoing events. To circumvent unproductive definitional arguments, methodologies that track large-scale violence according to numerically-based thresholds have substituted for dynamics-based analyses. Able to address aspects of the genocide puzzle, these methodologies struggle to incorporate cross-cultural contextual variation or elicit ripe moments for specific, real-time …

Systems And Methods For Detecting Alcohol, Acetone, And Carbon Monoxide In Breath, Tiffany Crystal Miller, Salvatore Domenic Morgera, Stephen Edward Saddow, Arash Takshi

USF Patents

A breath collection device can detect changes associated with pathogenesis of a disease, such as COVID-19, including biomarkers of immune response for respiratory symptoms, central nervous system injury, and/or peripheral nervous system injury in user breath and/or odor. The breath collection device can detect concentrations of alcohol, acetone, and carbon monoxide in user breath samples. A breath sample can be received in an internal bladder of the device for sensor analysis. Concentrations of alcohol, acetone, and carbon monoxide can be determined by calibrated calculation. A detection method for alcohol, acetone, and carbon monoxide can provide a non-invasive, rapid, and selective …

Tapping Into Food Industry: A Comparative Dual-Model Study Of Religion And Culture On Food Products’ Purchase Intention, Sumera Syed, Fauziah S. Ahmad, Syed R. H. Shah

Journal of Mediterranean Tourism Research

Due to increased trend of globalization and immigration to advanced countries during the past decade, Halal food consumers are being observed not only in Islamic countries but also in Western European countries. Moreover, the concepts of hygiene, quality and cleanliness, being often related to Halal food, make it equally favorable for consumption to non-Muslims. However, this vast area of Halal food sector still needs further exploration. Though recent studies have, to some extent, tried to explore the impact of religiosity on Halal food purchase intention, but still the impact of religion on Halal food purchase intention remains unexplored. Moreover, almost …

Proceedings Of The Global Conference On Education And Research: Volume 4, Waynne B. James, Cihan Cobanoglu, Muhittin Cavusoglu

University of South Florida (USF) M3 Publishing

This is the fourth volume of the Global Conference on Education and Research Proceedings Series.

ISSN: 2572-6374

DOI

10.5038/2572-6374-v4

An Exploratory Study On Cognitive Internship Perception Of Tourism Students, Ozge Buyuk, Gulsah Akkus

University of South Florida (USF) M3 Publishing

Tourism and hospitality education fills the need of skilled employees for tourism industry. Turkey has many vocational schools which provide the qualified personnel need. Students training in these institutions concerned carry out compulsory internships in tourism enterprises. Internship is a beneficial process that leads students the opportunity to transform their theoretical knowledge into practice. However, many problems exist during the internship. During this period, interns recognize both the sector and themselves further. Positive perceptions of internship period increase the willingness of students to work in the tourism industry after graduation. So defining the students’ perceptions and complaints during the internship …