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Molecular Mechanisms Governing Osteosarcoma Pulmonary Metastasis, Niveditha Nerlakanti

USF Tampa Graduate Theses and Dissertations

Osteosarcoma (OS) is a bone cancer that commonly metastasizes to the lung. Despite advances in the development of targeted therapies for treating solid malignancies, broad-acting chemotherapies remain the first line of treatment for OS. In assaying the efficacy of approved therapeutics for non-OS malignancies, we identified the histone deacetylase 1 and 2 (HDAC1 and 2) inhibitor, romidepsin, as effective for treating established lung metastatic OS. However, romidepsin has noted toxicities in humans, thus we aimed to define the primary mechanisms through which HDAC1/2 mediates OS progression to identify more selective druggable targets/pathways. Microarray and proteomic analyses of romidepsin-treated OS cells …

Leveraging Feedback From Older Adults To Enhance Patient-Centered Engagement In Healthcare Services, Erica Susan Sappington

USF Tampa Graduate Theses and Dissertations

This dissertation is comprised of an introduction to the topic, three academic papers, and a final chapter discussing the implications of the findings. Its primary objective is to examine strategies to engage older adults in proactive health interventions, enhancing their participation and satisfaction within healthcare systems. Each academic paper utilized distinct data sources and research methods. Paper 1's survey data were analyzed using descriptive statistics and correlations to understand older adults' engagement with academic medical centers. Paper 2 used thematic analysis of focus group data to identify key themes in older adults' perspectives on novel health services. Paper 3 used …

Systems And Methods For Detection Of Chocolate Bloom, Joshua Stewart Heuler, Siyu He, Sharad Ambardar, Dmitri V. Voronin

USF Patents

In an embodiment, a bloom engine is created that can detect the formation of both fat bloom and sugar bloom in a chocolate product. A Raman spectrometer is used to generate a first or reference spectrum of a chocolate product. Based on peaks of the spectrum known to be associated with sugar and peaks of the spectrum known to be associated with fat, a ratio of sugar to fat for the chocolate product is determined based on the reference spectrum. Later, a second spectrum is generated from the chocolate product, and a ratio of sugar to fat is similarly determined …

Plasma Derived From Human Umbilical Cord Blood For The Treatment Of Neurodegenerative Disorders, Svitlana Garbuzova-Davis, Jared Carl Ehrhart, Paul R. Sanberg

USF Patents

A method of treating neurodegenerative diseases using hUCB plasma is presented herein. hUCB plasma attenuated the hyperactive response (Group III) and potentiated the normal response in Group I ALS patients, but did not alter that of the non-responders to PHA (Group II). The elevated activity of caspase 3/7 observed in the MNCs from ALS patients was significantly reduced by hUCB plasma treatment. The ability of hUCB plasma to modulate the mitogen cell response and reduce caspase activity suggest that the use of hUCB plasma alone, or with stem cells, may prove useful as a therapeutic in ALS patients. hUCB plasma …

Understanding Contributing Factors To Injury Severity And Improper Driver Actions In Work Zone Crashes And Evaluating Smart Work Zone Applications For Safety Improvement, Rakesh Rangaswamy

USF Tampa Graduate Theses and Dissertations

A work zone can be described as a roadway designated for construction, maintenance, or other activities that temporarily disrupt the normal traffic flow. Due to this disrupts in traffic conditions, work zones may bring sudden shifts in driving experience, depending on the sort of work zone present, creating unpredictable maneuvers, and putting both motorists and workers at risk. To understand these issues, this study uses work zone accident data obtained from Florida and uses several methodological techniques, including the mixed logit model with variance and mean heterogeneity, temporal instability test, log-likelihood test, out-of-sample analysis, and ANOVA test.

Firstly, this study …

“Who Am I?”: Novice Teachers Making Meaning Of Their Intersectional Teacher Identity, Kerrijo E. Ellis

USF Tampa Graduate Theses and Dissertations

The purpose of this qualitative study was to explore how marginalized and/or minoritized novice teachers make meaning of their teacher identity. An integrated theoretical framework informed this study using Hong et al.’s (2024) conceptualization of teacher identity, Crenshaw’s (1989) intersectionality, and Collins & Bilge’s (2020) definition of intersectionality. Utilizing the photovoice methodology, four focus groups were conducted with a purposeful sample of five novice teachers who identified as marginalized (i.e., gender, disability, LGBTQIA+) and/or minoritized (i.e., ethnic and/or racial minority). Using reflexive thematic analysis (Braun & Clarke, 2023), I identified three themes: (1) Why I Became a Teacher, which included …

Feminist Narrative Inquiry: Stories Of Five Young Adults Who Aged Out Of Florida Foster Care, Amy E. Nourie

USF Tampa Graduate Theses and Dissertations

There are approximately 400,000 children in foster care in the United States and, each year, more than 20,000 reach the age of majority without achieving permanency, also known as aging out (Annie E. Casey Foundation, 2022a). Research has consistently called attention to the negative effects of foster care on youth including issues relating to substance use, housing instability, educational obstacles, and mental health concerns (Forkey & Szilagyi, 2014; Sarri et al., 2016). These negative outcomes are often even more pronounced for young adults aging out of foster care and transitioning into adulthood (Haggman-Laitila et al., 2019). Despite the decades of …

Performance And Trihalomethane Formation Potential Of Alternative Electron Donors For Wastewater Denitrification, Bruno Fulco Mancini

USF Tampa Graduate Theses and Dissertations

Nitrogen removal from wastewater is critical to preserve ecosystems, as well as human health and welfare. Excess nutrients in receiving waters can cause many problems to the environment, such as algal blooms, drinking water contamination, and economic losses. Nitrogen removal in wastewater occurs mainly through the oxidation and reduction processes of nitrification and denitrification, which occur in engineered systems at wastewater treatment plants or water reclamation facilities.

The site for this research was the South Cross Bayou (SCB) Water Reclamation Facility, located in Pinellas County, FL, USA. At SCB, nitrogen is removed through a Modified Ludzak-Ettinger (MLE) process with the …

A Contexted Learning Experience: Instructional Strategies For Effective Teaching For Primary And Intermediate Students From Diverse Ses Environments, R. Suzanne Legg

USF Tampa Graduate Theses and Dissertations

The aim of my three-article dissertation was to investigate the methods employed by effective teachers to promote academic progress among all students, regardless of their socioeconomic status. Each article addressed perspectives on effective teaching strategies aimed at closing the opportunity gap from three different contexts. These contexts were 1) a focus group consisting of primary teachers, 2) a focus group consisting of intermediate teachers, and 3) personal reflections from observations of both focus groups pertaining to COVID-19. Recent scores from the Programme for International Student Assessment (OECD, 2023) revealed a growing disparity in academic achievement between students from higher socioeconomic …

Stochastic Predictive Modeling Of Social Media Stocks And Reliability Of Hard Disk Drives, Sameer Anand

USF Tampa Graduate Theses and Dissertations

In recent years, social media has emerged as a highly appealing investment sector due to its substantial returns. Our research focuses on creating a predictive model for the financial performance of social media companies. We gathered data from five companies in the S&P 500: Meta, Etsy, Match, Pinterest, and Alphabet. We developed a non-linear, data-driven stochastic analytical model that employs real data, incorporating six financial indicators and four economic indicators, to forecast the weekly closing prices of these social media companies with an accuracy of 94.7%. Additionally, we ranked the statistically significant indicators and their interactions based on their percentage …

Evaluating The Effectiveness Of Edge Line Sinusoidal Rumble Strips On Lane Departure Crash Prevention And Injury Severity, Shubhankar Chintamani Shindgikar

USF Tampa Graduate Theses and Dissertations

Lane departure crashes are one of the most prevalent accidents in Florida and the United States. Various forms of rumble strips have been employed to mitigate and decrease these incidents. The Florida Department of Transportation (FDOT) recently adopted a new sinusoidal rumble strip pattern for statewide implementation of audible and vibratory treatment (AVT). Recent research indicates sinusoidal rumble strips are highly effective in alerting drivers audibly and through tactile vibrations, thus aiding lane departure prevention. Moreover, they generate less external noise compared to other rumble strip textures.

However, a systematic assessment of the safety effectiveness of sinusoidal rumble strips in …

Efficient Smartphone Sensor Analysis For Behavioral Profiling In Transportation Research: A Case Study Of Driver And Passenger Classification, Wilson Lozano, Tempestt Neal

Research Reports

Our study investigates smartphone sensor data for classifying drivers and passengers, aligning with our original proposal to enhance multimodal transportation data collection via the OneBusAway app. We address challenges in balancing data quality with smartphone resource constraints and find that shorter data collection windows significantly improve classification performance. Additionally, feature standardization and selection enhance accuracy, particularly during extended data periods, supporting our goal of refining data quality from open-source platforms. These insights advance our objectives by optimizing data efficiency and supporting the real-world applicability of intelligent models in transportation safety and efficiency.

Phosphoglycerate Dehydrogenase Regulated By The Mitogen-Activated Protein Kinase Pathway Is An Actionable Vulnerability In Cutaneous Melanoma, Neel Jasani

USF Tampa Graduate Theses and Dissertations

Metabolic alterations play a critical role in shaping tumor growth and development. Many metabolic enzymes are shown to play an important role in different aspects of tumor development, growth, and metastasis. One such enzyme that we are investigating in this project is the Phosphoglycerate Dehydrogenase (PHGDH). The PHGDH gene, encoding the rate-limiting enzyme of the serine synthesis pathway, is located at chromosome locus 1p12 and frequently amplified in melanoma. PHGDH is required for the proliferation of melanoma cells with chromosome 1p12 amplification. However, the role of PHGDH in chromosome 1p12 non-amplified melanoma is poorly understood. In this project, we sought …

Examining Emergency Remote Teaching And Undergraduate Academic Outcomes During The Pandemic, Melissa S. Ramge

USF Tampa Graduate Theses and Dissertations

The COVID-19 pandemic represented an extraordinary public health emergency, leading to the most profound disruption to education systems in modern history. As higher education institutions expand their post-pandemic online course catalogs, it is necessary first to explore the student outcomes and student perceptions of learning related to the pandemic. This study used an explanatory sequential mixed methods research design to assess the relationship between the COVID-19 pandemic and students’ perceptions of online learning. The purpose of the study was to examine what, if any, trends in undergraduate student course grades and student perceptions of the instruction occurred coincident with the …

The Monsters In Twentieth And Twenty-First Century Illustrated Adaptation Of Beowulf For Children And Young Adults, Johanna Katharine Burton

USF Tampa Graduate Theses and Dissertations

I examine how some authors and illustrators of children’s and young adult literature adapt the old English Beowulf, how critics receive their works, and the functions these adaptations serve. My focus is on how the interplay between the visual and verbal text modifies or amplifies the overall story as well as how theories of translation/adaptation, monster, gender, and post-colonialism provide insight and aid in analysis. My ultimate objective is to demonstrate the significance of children’s and young adult adaptations of Beowulf and how these texts contribute to a rich and varied corpus. I argue that the adapter’s social/cultural context affected …

Communication Resilience In Suicide Prevention (Crisp): An Institutional Ethnography Of Community-Based Suicide Prevention In Florida, Kelli R. Agrawal

USF Tampa Graduate Theses and Dissertations

The concept of Communication Resilience in Suicide Prevention (CRISP) offers a conceptual framework for exploring communication strategies in community-based suicide prevention. A constructivist paradigm informs this study and utilizes an approach heavily influenced by Dorothy Smith’s critical feminist sociological work of institutional ethnography (IE). The study intentionally interweaves critical research praxis with a dual etic/emic positionality through constructivist understandings of dialectical sensemaking. It uses the Communication Theory of Resilience (CTR) and Institutional Logics Theory (ILT) as analytic tools of inquiry. The study includes purposive sampling and multiple qualitative methods, including unstructured interviews, text analyses, and observational data. Fifteen unstructured interviews …

Targeting Tumor Microenvironmental Factors In Bladder Cancer To Enhance Responses With Immunotherapy, Sarah Bazargan

USF Tampa Graduate Theses and Dissertations

Bladder cancer is the tenth most common cancer in the world and has one of the highest recurrence rates. Despite the local delivery options and mutational status of bladder tumors, responses with immunotherapies have been limited with low durability. The bladder tumor microenvironment is enriched with highly immunosuppressive populations and harsh conditions that restrict anti-tumor immunity and subsequently attenuate immunotherapy response. We predict that targeting two of these immunosuppressive components, namely acidity and myeloid derived suppressor cells (MDSCs), will bolster T cell responses and enhance outcomes with immunotherapy. In Chapter 2, we characterized the effects of acidity on T cell …

Infiltration Of Immune Cells And Lipid Nanoparticle-Mediated Drug Delivery In A Skin Cancer Model, Aynur Sanem Yilmaz

USF Tampa Graduate Theses and Dissertations

Melanoma, the deadliest form of skin cancer, is notorious for its rapid mutation rate and high potential for metastasis, making effective treatment particularly challenging. Immunotherapy has emerged as a significant advancement in melanoma therapy, leveraging the immune system to target and eliminate cancer cells. These achievements have motivated further investigation into immune system-based strategies for this aggressive cancer. Cell-based drug delivery has been primarily achieved by either using immune cells as direct carriers for therapeutics or by employing active molecules through drug delivery systems, most often nanoparticles. While immune cells exhibit innate tumor-homing abilities, they face challenges such as limited …

Learning Peer Support Interactions Via Bi-Lstm Graph Neural Networks For Suicide Risk Prediction, Harikrishna Marampelly

USF Tampa Graduate Theses and Dissertations

Suicide prevention through early detection using social media data has been widely studied. However, the critical role of peer support interactions among individuals with similar mental disorders has not been deeply investigated or explored. In this study, we explore peer interactions in online communities for individuals with bipolar disorder and leverage this information to predict suicide risk levels. We propose a model that uses contextualized posts and comments along with their sentiment features. By embedding these features into a peer support network, our model captures peer interactions and predicts suicide risk levels using the bidirectional LSTM Graph Neural Networks (Bi-LSTM …

Vulnerability Discovery On Broadcom Devices With Qemu, Christian Shane Douglas Little

USF Tampa Graduate Theses and Dissertations

System-on-chip (SoC) devices in the form of routers, access points, and smart appliances have become an increasingly prevalent aspect of our infrastructure. Performing vulnerability research on these devices may be exceptionally difficult given the on-hardware limitations, inability to guarantee certain aspects of the system, and lack of information when the appliance’s source code is not available.In this thesis, we present Broadfeather, a QEMU-based emulation target, and framework for dynamic analysis and instrumentation. While Broadfeather supports data types shared across many chips under the Broadcom umbrella, it is primarily geared towards the BCM6755 Wi-Fi 6E capable chipset. Broadfeather also features the …

Pseudomonas Aeruginosa Aggregate Formation In The Cystic Fibrosis Airway, Alexa D. Gannon

USF Tampa Graduate Theses and Dissertations

Bacterial aggregates are observed in both natural and artificial environments. In the context of disease, aggregates have been isolated from both chronic and acute infections. Pseudomonas aeruginosa (Pa) aggregates contribute significantly to chronic infections, particularly in the lungs of people with cystic fibrosis (CF). Unlike the large biofilm structures observed in vitro, Pa in CF sputum forms smaller aggregates (~10-1000 cells), and the mechanisms behind their formation remain underexplored. This study aims to identify genes essential and unique to Pa aggregate formation in a synthetic CF sputum media (SCFM2). We cultured Pa strain PAO1 in SCFM2 and LB, both with …

Chikungunya Pathogenesis In Cardiac Tissues And Its Evaluation In Stem Cell Derived Cardiac Models, Elizabeth M. Traverse

USF Tampa Graduate Theses and Dissertations

Chikungunya Virus (CHIKV) is a single stranded RNA alphavirus primarily vectored by Aedes aegypti and Aedes albopictus mosquitos. CHIKV has circulated in tropical and sub-tropical regions of Africa and Asia, but outbreaks in the past two decades have seen the virus spread to Europe, South America, and the Caribbean. Disease from CHIKV infection typically results in fever, malaise, and sometimes debilitating arthralgia. However, there are concerning abnormal presentations that affect the human heart, including myocarditis, heart failure, and death. These symptoms are seen in all age groups and sexes and are not connected to particular strains or mutations in the …

A Phenotypic Characterization Of Staphylococcus Aureus Bacteremia Clinical Isolates, Sara Dj Daniels

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a human pathogen of serious concern because of its ability to cause serious and wide-ranging disease. Of particular concern is the ability of S. aureus to cause bacteremia (SAB), allowing the bacteria to disseminate and spread to various organs. This study addressed this issue through the phenotypic characterization of two methicillin-resistant S. aureus (MRSA) clinical isolates taken from patients with sustained complicated bacteremia at Tampa General Hospital, referred to as SA1097 and SA1098. A battery of phenotypic tests was conducted on these strains, including a growth curve, hemolysis and proteolysis screenings, cytotoxicity assays using human neutrophils, blood …

Expert Consensus In A Politically-Charged Environment: Motivated Reasoning In Information Processing, Austin L. Katz

USF Tampa Graduate Theses and Dissertations

With the abundance of information online, what drives news consumption? It has been theorized that there are two distinct conflicting motivations which operate simultaneously when consumers encounter new information: the motivation to reach accurate conclusions and the directional motivation to reach conclusions that are consistent with a person’s preexisting beliefs or predispositions (e.g., Kunda 1990). This experiment tests situations in which both motivations are simultaneously made salient in the context of reading headlines to decide what news to read and how accurate the news is likely to be.

A new fact-check manipulation was used to draw attention to the accuracy …

Selective Pericyte Recruitment To Ec-Lined Tubes, Vessel Assembly Enhancement Through Pericyte Timp-3 Induction, And Pericyte Tbx2 In Capillary Formation And Maturation, Ksenia Marilyn Yrigoin Kaluguina

USF Tampa Graduate Theses and Dissertations

Capillaries, the smallest blood vascular networks, are formed by narrow and elongated endothelial cell (EC)-lined tubes with associated pericytes anchored to the abluminal tube surface. Our laboratory demonstrated that dynamic interactions between ECs and pericytes during capillary tube network assembly are required for basement membrane matrix deposition and maturation. We have also shown that key EC-derived factors promote pericyte recruitment, proliferation, and pericyte-induced basement membrane formation during capillary assembly. In the first section, we aim to identify if vascular smooth muscle cells (VSMCs) respond to the same factors and have the same invasive ability as pericytes since they are both …

Operational Variations In State-Owned Hotels And International Branded Hotels In China: A Qualitative Analysis Of Practitioners' Perspectives And Implications, Wen Wei

USF Tampa Graduate Theses and Dissertations

This study examines experienced hoteliers' perspectives on essential operational practices for delivering consistent, high-quality customer experiences, focusing on operational differences between Chinese State-Owned Hotels (SOHs) and International Branded Hotels (IBHs) in China. Through semi-structured interviews with 24 experienced hoteliers from SOHs and IBHs, the research identifies operational practices in IBHs that are perceived as more effective than those in SOHs. Additionally, the study explores operational practices that SOHs and IBHs could adopt and adapt to succeed in China’s post-pandemic hotel market. The analysis is framed by the Resource-Based View (RBV), Dynamic Capabilities (DCs), Market Orientation (MO), and the role of …

The Attitudes, Perceptions, And Motivations Of Student-Athletes In Intermediate Algebra, Alexandra Green

USF Tampa Graduate Theses and Dissertations

This research delves into the attitudes, perceptions, and motivations of student-athletes enrolled in Intermediate Algebra courses. It focuses on the distinct challenges they encounter in juggling academic and athletic responsibilities at a southeastern university in the United States. Employing a mixed-methods approach, the study gathered quantitative data through surveys to assess students' attitudes, perceptions, and motivations toward learning mathematics, as well as to determine the influence of their instructors on these factors. The qualitative segment provided deeper insights into their personal experiences, shedding light on their prior encounters with mathematics, their most memorable moments during the current course, and their …

Revision Strategies And Academic Socialization Of Multilingual Graduate Students, Sean E. Farrell

USF Tampa Graduate Theses and Dissertations

Research suggests that revision is a complex practice guided by a variety of factors; however, the nature of the relationship between these factors and the revisions themselves, along with how the revision process contributes to academic socialization, remains understudied. A more comprehensive understanding of revision can lead to a deeper understanding of the support that will be most effective for multilingual graduate students. This study thus aims to determine (1) what revision strategies multilingual graduate students use, (2) what factors mediate these strategies, and (3) the extent to which these revisions index a shift in academic socialization. To answer these …

Essays On Information Technology In Healthcare, Gleb Zavadskiy

USF Tampa Graduate Theses and Dissertations

Information technologies (IT) and information systems (IS) have profound significance across various sectors of society, including healthcare, business, education, government, and beyond. First, IT facilitates instant communication globally through email, messaging apps, video conferencing, and social media, revolutionizing how individuals and organizations interact, collaborate, and share information (Hacker et al. 2020; Tang and Hew 2020).

Secondly, the Internet and digital libraries provide worldwide access to vast amounts of information, what makes knowledge and education available to everyone, empowering individuals to learn and stay informed on diverse topics (Haleem et al. 2022). Another aspect of IT systems in various industries is …

Computer Networking With Security Features, Mohamed Rahouti, Kaiqi Xiong

USF Patents

Described herein relates to a system and method for the prevention of a malicious attack on a computing resource. In embodiments, the system may comprise the following, including but not limited to: (1) at least one processor; and (2) computer memory storing instructions that, when executed by the at least one processor, cause the at least one processor to perform operations including: (a) observing traffic flow of a network; (b) altering a SYN threshold value based on the observing of the traffic flow of the network; (c) comparing a metric of SYN messages submitted to the network; and (d) based …