Digital Commons Network^™

A Functional Characterization Of The Omega (Ω) Subunit Of Rna Polymerase In Staphylococcus Aureus, Shrushti B. Patil

USF Tampa Graduate Theses and Dissertations

In bacteria, RNA polymerase (RNAP) is a well-characterized and highly conserved multi-subunit enzyme complex responsible for transcription of DNA into RNA. The ω subunit (rpoZ/RpoZ), one of the smaller accessory subunits of RNAP, is often overlooked and under-studied, however, particularly in Gram-positive species. We have previously shown that deleting ω impacts the integrity of RNA polymerase in S. aureus, especially the β' subunit, and alters preference of the core enzyme for sigma factors, skewing heavily towards σB instead of the housekeeping σ factor. Consequently, this causes deregulation of myriad transcriptional processes, strongly rewiring gene expression circuits, and ultimately impairing the …

The Weekly Challenger, October 28, 2021, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The October 28, 2021, issue of The Weekly Challenger.

Quercetin Loaded Nanoparticles For The Treatment Of Dry Eye Disease, Bassma Sami Nashaat Hamed Eltanameli

USF Tampa Graduate Theses and Dissertations

Dry eye disease (DED) is a common condition that affects the eye's anterior surface due to tear deficiency or excessive evaporation. The lack of tears leads to dryness and initiation of an inflammatory response which can irritate the eye and interfere with daily activities. Current treatment depends mainly on artificial tears eye drops which offer temporary relief and require repetitive administration due to frequent blinking. Prescription medications are also available but need around three months to relieve dry eye symptoms. Quercetin is a flavonoid that has an anti-inflammatory and antioxidant effect. However, it suffers from low water solubility and low …

The Media Reproduction Of Racial Violence: A Content Analysis Of News Coverage Following The Death Of George Floyd Jr., Keylon Lovett

USF Tampa Graduate Theses and Dissertations

The media has played a critical role in reproducing anti-Black violence in the United States, which has often harmed African American communities. Historically, the white press has depicted graphic imagery and descriptions of Black people being brutalized, with little ethical regard to their harmful effects. The Black press has historically challenged negative portrayals in the white media and shown more nuance, to protect the Black audience it represents. This dynamic underpins media depictions of racial violence still seen today. Darnella Frazier’s video capture of George Floyd’s death by Minneapolis police, was widely shared in the weeks following the incident, across …

The Multifaceted Role Of Ccar-1 In The Alternative Splicing And Germline Regulation In Caenorhabditis Elegans, Doreen Ikhuva Lugano

USF Tampa Graduate Theses and Dissertations

The Cell Division Cycle and Apoptosis Regulator (CCAR) family members are an enigmatic family of proteins regulating metabolism, cancer, apoptosis, DNA damage, and stress. Mammals have CCAR family members, CCAR1 and CCAR2/DBC1, which evolved from the founding family member CCAR-1/LST-3 expressed in Caenorhabditis elegans. Several studies have shown the importance of understanding these proteins' function in standard and altered physiological processes. Our studies aim to understand the genome-wide alternative splicing and germline regulation of Caenorhabditis elegans CCAR-1 in normal and heat shock conditions. Recently, mammalian CCAR family member CCAR2/DBC1 regulates the alternative splicing by forming a complex with ZNF326. This …

Criminalizing Lgbtq+ Jamaicans: Social, Legal, And Colonial Influences On Homophobic Policy, Zoe C. Knowles

USF Tampa Graduate Theses and Dissertations

Based on colonial and neocolonial models of oppression, Jamaica has adopted many laws, policies, and systems mandated by the British monarchy. Many of these laws contain anti-LGBTQ+ policies which remain in effect today. To address the criminalization of LGBTQ+ identities, I used queer theory and queer criminology to analyse the ways Jamaica constructs LGBTQ+ people as criminals and how they are treated in the legal and criminal justice systems from a postcolonial standpoint. Using a qualitative text-based feminist and queer policy analysis, I investigated social, legal, and colonial influences on current anti-LGBTQ+ policy by looking at the Offences Against the …

Informing Complexity: The Business Case For Managing Digital Twins Of Complex Process Facilities As A Valuable Asset, William Randell Mcnair

USF Tampa Graduate Theses and Dissertations

The Digital Twins of complex facilities, specifically 3D models created during their design, is a potentially valuable information asset. This three- article dissertation explores the business case for firms in the petrochemical process industry to manage throughout the facility lifecycle. A maturity model is provided to illustrate the stages of digital twin evolution and serves as a tool to help communicate each of the five levels of digital twin maturity achievable in various use cases. An industry analysis reviews existing literature and proposes a model to assess informing or insight value of digital twins from three perspectives. Next, an empirical …

Understanding Transantiago Users’ Motivations For Paying Or Evading Payment Of Bus Fares, Carolina Busco, Felipe González, Yamil Jaqueih, Felipe Jiménez, Borja Alonso

Journal of Public Transportation

Transantiago, the public transport system implemented in Santiago, Chile, remains a controversial subject of public debate due to constantly increasing fare evasion rates throughout its decade-long existence. The

Transantiago, the public transport system implemented in Santiago, Chile, remains a controversial subject of public debate due to constantly increasing fare evasion rates throughout its decade-long existence. The research question under consideration in this paper is as follows: What motivates individuals to evade or pay bus fares? To answer this question, we developed a multidisciplinary study that combined relevant engineering expertise with a sociological perspective and combined quantitative analyses with qualitative methodologies …

Restarting Plural Modernity: The Lyrical Tradition Of The Hometown In Kaili Blues, Huadong Fan

USF Tampa Graduate Theses and Dissertations

The dichotomous paradigm constituted by the realist tradition of Chinese cinema has become the epic and inertial discourse for understanding Chinese modernity. However, I argue that some new filmmakers after the sixth generation of directors have used the narrative and aesthetics of the hometown to change this dominant logic of Chinese modernity. The revolutions and wars of the twentieth century transformed the hometown from a relatively stable agrarian civilization to a modern society in violent turmoil. Recent hometown films are no longer satisfied with this realist aesthetic duality and strive for a pluralistic discourse of Chinese modernity, releasing it from …

Computer Simulation For Continuous Centrifugation Of High-Density Cell Cultures, Hironori Tomizawa

USF Tampa Graduate Theses and Dissertations

Perfusion bioreactors are used for cell production for cell therapies. The system uses cell separation devices such as centrifuge to facilitate cell growth by separating cells and spent media and allows constant media replacement. However, current cell separation technologies can increase shear stress, are generally not continuous processes, and are not as efficient as desired. These limit the amount of media replaced per unit time. Therefore, there is a need for a new system or a method that operates continuously to minimize cell damage, yield high cell concentration, and exchange media effectively. A set of research questions was formulated: (1) …

Cultural Values As A Moderator Of The Emotion Suppression To Strain Relationship: A Comparison Of Two Dominant Theoretical Mechanisms, Roxanne C. Lawrence

USF Tampa Graduate Theses and Dissertations

Although the relationship between emotion regulation and deleterious health outcomes is a robust finding in Western cultures, studies show that this effect is attenuated in non-Western cultures. The present study employed an experience sampling method to examine the mitigating effect of cultural values (i.e., individual-level collectivism) on the relationship between emotion regulation and employee strain (i.e., job satisfaction and anxiety) through the theoretical models of emotional dissonance and resource loss (operationalized as inauthenticity and emotional exhaustion). Using data collected from 182 adults working in the service industry, I ran a multilevel path analysis to test the study’s hypotheses. Results indicated …

Constructing Frameworks For Task-Optimized Visualizations, Ghulam Jilani Abdul Rahim Quadri

USF Tampa Graduate Theses and Dissertations

Visualization is crucial in today’s data-driven world to augment and enhance human understanding and decision-making. Effective visualizations must support accuracy in visual task performance and expressive data communication. Effective visualization design depends on the visual channels used, chart types, or visual tasks. However, design choices and visual judgment are co-related, and effectiveness is not one-dimensional, leading to a significant need to understand the intersection of these factors to create optimized visualizations. Hence, constructing frameworks that consider both design decisions and the task being performed enables optimizing visualization design to maximize efficacy. This dissertation describes experiments, techniques, and user studies to …

Crow's Nest, October 18, 2021, Crow's Nest Staff

Crow's Nest

The October 18, 2021, issue of the Crow's Nest.

Unintended Consequences? Testing The Effects Of Adolescent-Targeted Anti-Vaping Media Upon Adult Smokers, Leslie E. Sawyer

USF Tampa Graduate Theses and Dissertations

A great deal of controversy surrounds e-cigarettes, with some arguing that protection of youth and non-users is paramount and others maintaining that these products are beneficial from a harm reduction perspective for use by adult smokers for switching from combustible cigarettes and for smoking cessation. Opponents of e-cigarettes have allocated tremendous funds toward advertising campaigns aimed at youth deterrence; however, to date, the effects of these ads upon adult smokers have yet to be examined. The current study used a between-subjects experimental design to investigate the effects of an FDA-distributed youth-targeted anti-vaping PSA, “Vaping is an Epidemic,” upon adult smokers …

The Weekly Challenger, October 14, 2021, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The October 14, 2021, issue of The Weekly Challenger.

Crow's Nest, October 11, 2021, Crow's Nest Staff

Crow's Nest

The October 11, 2021, issue of the Crow's Nest.

Book Review: It Can Happen Here: White Power And The Rising Threat Of Genocide In The Us, Jeffrey Bachman

Genocide Studies and Prevention: An International Journal

No abstract provided.

Full Issue 15.2

Genocide Studies and Prevention: An International Journal

No abstract provided.

Dossier: The Stateless Rohingya—Practical Consequences Of Expulsion, Fiza Lee-Winter, Tonny Kirabira

Genocide Studies and Prevention: An International Journal

The international community has been called upon to ramp up efforts to end statelessness and provided with a guiding framework of 10 Actions. This dossier presents the practical consequences of expulsion, both direct and indirect outcomes of collective violence, directed towards the Rohingyas. Touching upon the nexus between children's rights, human trafficking, and practical challenges associated on-the-ground, the dossier also discusses the imperative need for the Association of Southeast Asian Nations (ASEAN) states—collectively as a region—to take steps in fulfilling Action 7 of the Global Action Plan through the birth registration of Rohingya children as part of their existing efforts …

Pinpointing Patterns Of Violence: A Comparative Genocide Studies Approach To Violence Escalation In The Ukrainian Holodomor, Kristina Hook

Genocide Studies and Prevention: An International Journal

This article utilizes the case study of the 1930s Ukrainian Holodomor, an artificially induced famine under Joseph Stalin, to advance comparative genocide studies debates regarding the nature, onset, and prevention of large-scale violence. Fieldwide debates question how to 1) distinguish genocide from other forms of large-scale violence and 2) trace genocides as unfolding processes, rather than crescendoing events. To circumvent unproductive definitional arguments, methodologies that track large-scale violence according to numerically-based thresholds have substituted for dynamics-based analyses. Able to address aspects of the genocide puzzle, these methodologies struggle to incorporate cross-cultural contextual variation or elicit ripe moments for specific, real-time …

Food Producers And Pandemics: A Mystery Shopping Analysis, Francesc Fusté-Forné

Journal of Sustainability and Resilience

Recent research has widely analysed the significance of food in tourism. Departing from the understanding of ‘cheese’ as part of the food tourism system of a destination, this paper aims to analyse cheese factory tours as a tourism service provided by food producers. A mystery shopping approach is used to study the 75 cheese producers under the Manchego quality cheese. Results show the response behaviour of Spanish Manchego cheese producers to an email sent by a ‘tourist’ who asks for a visit during pandemic times. As a segment of food tourism, cheese tourism is gathering a growing attention by academics …

A Future Of Tourism Industry: Conscious Travel, Destination Recovery And Regenerative Tourism, Asif Hussain

Journal of Sustainability and Resilience

The tourism industry is a complex network of millions of suppliers and consumers who trade experiences and services. The traditional tourism model revolves around increasing the number of visitors to ensure economic return. The conventional tourism models often ignore the real cost of the travel industry’s health and the cost per tourist. Covid-19 has shown us the extent to which the travel industry can be affected. The tourism and hospitality industries is the worst affected industries globally and the continuous waves of the virus, and new variants, are forcing governments to impose strict lockdowns. Misinformation and disinformation are making it …

Systems And Methods For Expert-Assisted Classification, Balaji Padmanabhan, Utkarsh Shrivastava, Vivek Kumar Singh

USF Patents

Systems and methods for expert-assisted classification are described herein. An example method for evaluating an expert-assisted classifier can include providing a cascade classifier including a plurality of classifier stages; and providing a simulated expert stage between at least two of the classifier stages. The simulated expert stage can be configured to validate or contradict an output of one of the at least two classifier stages. The method can also include classifying each of a plurality of records into one of a plurality of categories using the cascade classifier combined with the simulated expert stage; and determining whether the simulated expert …

Crow's Nest, October 4, 2021, Crow's Nest Staff

Crow's Nest

The October 4, 2021, issue of the Crow's Nest.

Usft Digital Scholarship Services Annual Report: Fy 2020-2021, Digital Scholarship Services

Digital Commons @ USF Publications

This is the annual report for FY 2020-2021, the fifth full year of Digital Scholarship Services (DSS).

The Rise Of The Cosmetic Industry In Ancient China: Insights From A 2700‐Year‐Old Face Cream, B. Han, J. Chong, Z. Sun, X. Jiang, Q. Xiao, J. Zech, P. Roberts, H. Rao, Y. Yang

KIP Articles

Cosmetics have a long history in China, but their origins remain unclear. Cosmetic industry potentially originated in the Spring and Autumn Period (770–476 bce ), but little is known about the early manufacture and use of cosmetics. The Liujiawa site, located on the southern edge of the Loess Plateau in northern China, was the late capital of the ancient Rui state in the early to mid‐Spring and Autumn Period. During the excavation, a sealed small and exquisite container with suspected cosmetic use was unearthed from tomb M49 belonging to a male associated with the aristocratic class. We report in this …

Genealib Archive 2021-10, Genealib

GENEALIB Archive

No abstract provided.

Usf School Of Architecture And Community Design, Wimauma Community Plan, October 2021, Taryn Sabia, Maggie Winter

School of Architecture and Community Design

The Hillsborough County and the Planning Commission launched the Wimauma long-range community design process to update the 2008 Community Plan. The initiative emphasizes participatory design and consensus building while aiming to guide future growth, coordinate investments, and provide a policy framework for revitalizing Wimauma Village Residential-2 with a clear, shared community vision.

University Archives: Business & Implementation Plan, Andrew Huse, Tomaro Taylor

Special Collections Reports & Documents

No abstract provided.

Program, 32nd Annual Tampa Bay International Gay And Lesbian Film Festival, October 1-10, 2021, Friends Of The Festival, Inc.

Tampa Bay International Gay & Lesbian Film Festival (TIGLFF) Collection

Program for the 32nd Annual Tampa Bay International Gay and Lesbian Film Festival, held between October 1-10, 2021. Includes committee listing, advertisements, letters of welcome, sponsor information, festival jury, schedule, and film descriptions.