Digital Commons Network^™

Detection Of Novel Sars-Like And Other Coronaviruses In Bats From Kenya, Suxiang Tong, Christina Conrardy, Sujsan Ruone

KIP Articles

Diverse coronaviruses have been identified in bats from several continents but not from Africa. We identified group 1 and 2 coronaviruses in bats in Kenya, including SARS-related coronaviruses. The sequence diversity suggests that bats are well-established reservoirs for and likely sources of coronaviruses for many species, including humans.

Exploring The Secrets Of The Three-Dimensional Architecture Of Phototrophic Biofilms In Caves, Mònica Roldán, Mariona Hernández-Mariné

KIP Articles

Caves with dim natural light, and lighted hypogean environments, have been found to host phototrophic microorganisms from various taxonomic groups. These microorganisms group themselves into assemblies known as communities or biofilms, which are associated with rock surfaces. In this work, the phototrophic biofilms that colonise speleothems, walls and floors in three tourist caves (Spain) were studied. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were used to study these organisms and acquire three-dimensional data on their biofilm structure. CLSM was used in a multi-channel mode whereby the different channels map individual biofilm components. Cyanobacteria, green microalgae, diatoms, mosses …

Emergency Department Capacity Planning For A Pandemic Scenario: Nurse Allocation, Florentino Antonio Rico

USF Tampa Graduate Theses and Dissertations

The problem considered in this research is the efficient allocation of resources in an emergency department during a large flow of patient consequent to a pandemic influenza breakout. Predicting the impact of a Pandemic Influenza is very complex due to the many unknown variables that may play a role to how severe a pandemic can be. Scenario planning is considered in this research to forecast different potential outcomes and help decision makers better understand the role of uncertainties and become prepared to make important decisions. The goal is to first create a forecast model to estimate the patient demand during …

Protein Intrinsic Disorder And Influenza Virulence: The 1918 H1n1 And H5n1 Viruses, Gerard Kian-Meng Goh, A. Keith Dunker, Vladimir N. Uversky

Molecular Medicine Faculty Publications

Background: The 1918 H1N1 virus was a highly virulent strain that killed 20–50 million people. The cause of its virulence remains poorly understood.

Methods: Intrinsic disorder predictor PONDR® VLXT was used to compare various influenza subtypes and strains. Three-dimensional models using data from X-ray crystallographic studies annotated with disorder prediction were used to characterize the proteins.

Results: The protein of interest is hemagglutin (HA), which is a surface glycoprotein that plays a vital role in viral entry. Distinct differences between HA proteins of the virulent and non-virulent strains are seen, especially in the region near residues 68–79 of the HA2. …

Further Decline In Nest-Box Use By Vaux's Swifts In Northeastern Oregon, Evelyn L. Bull, Charles T. Collins

Western Birds

No abstract provided.

2009 Annual Report Usf System, Usf

USF Accountability Reports

No abstract provided.

The Political Ecology Of Intestinal Parasites Among Nicaraguan Immigrants In Monteverde, Costa Rica, Jason D. Lind

USF Tampa Graduate Theses and Dissertations

Over the past 15 years Monteverde, Costa Rica has undergone rapid economic, social, political, and environmental change due to a flourishing ecotourism economy. While the effects of ecotourism development in Monteverde are many, two important consequences have been: 1) the immigration of Nicaraguan nationals to the area seeking low-skilled wage labor; and 2) compromised water resources management due to pollution and rapid population growth. The objective of this research is to investigate and identify the inter-relationships between ecotourism development in Monteverde and its affect on infectious diseases outcomes within the context of immigration and water resources management. Specifically, this dissertation …

Maternal Knowledge, Attitudes And Practices And Health Outcomes Of Their Preschool-Age Children In Urban And Rural Karnataka, India, Angela Lloyd

USF Tampa Graduate Theses and Dissertations

This cross-sectional, community-based study was designed to compare the health outcomes of 2 - 5 year-old children in different types of preschools. The Integrated Child Development Services (ICDS), run by the government of India, created a system of preschools, called anganwadis, to combat malnutrition, provide health education for mothers, and preschool for children 2 - 6 years old in 1975. Many children attend their local anganwadis, while others attend private schools, and others do not attend school at all. A pre-tested questionnaire was used to interview 125 urban and 130 rural mothers regarding their knowledge, attitudes, and practices about acute …

Functional Characterization Of Cytochrome B5 Reductase And Its Electron Acceptor Cytochrome B5 In Plasmodium Falciparum, Lucio Malvisi

USF Tampa Graduate Theses and Dissertations

Malaria is a disease of major public health importance, killing approximately one million people and causing about 250 million cases of fever annually. It mostly affects children under the age of five and pregnant women in many developing countries, making it a prominent issue in international health and maternal and child health. The most aggressive form of malaria is caused by the parasite Plasmodium falciparum which is responsible for 80% of infections and 90% of deaths from malaria, and is most prevalent in sub-Saharan Africa. Public Health interventions include the implementation of prevention programs, health education, and chemotherapy. The latter …

Tthe Rad9-Rad1-Hus1 Complex And Bif-1 Regulate Multiple Mechanisms That Affect Sensitivity To Dna Damage, Cheryl L. Meyerkord

USF Tampa Graduate Theses and Dissertations

The resistance of cancer cells to traditional chemotherapeutic agents is a major obstacle in the successful treatment of cancer. Cancer cells manipulate a variety of signaling pathways to enhance resistance to anticancer agents; such mechanisms include disrupting the DNA damage response and hyperactivating survival signaling pathways. In an attempt to better understand the molecular mechanisms that underlie resistance to chemotherapeutic agents, we investigated multiple processes regulated by the Rad9-Rad1-Hus1 (9-1-1) complex and Bif-1. The 9-1-1 complex plays an integral role in the response to DNA damage and regulates many downstream signaling pathways. Overexpression of members of this complex has been …

Exploring The Secrets Of The Three-Dimensional Architecture Of Phototrophic Biofilms In Caves, Mònica Roldán, Mariona Hernández-Mariné

International Journal of Speleology

Caves with dim natural light, and lighted hypogean environments, have been found to host phototrophic microorganisms from various taxonomic groups. These microorganisms group themselves into assemblies known as communities or biofilms, which are associated with rock surfaces. In this work, the phototrophic biofilms that colonise speleothems, walls and floors in three tourist caves (Spain) were studied. Confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM) were used to study these organisms and acquire three-dimensional data on their biofilm structure. CLSM was used in a multi-channel mode whereby the different channels map individual biofilm components. Cyanobacteria, green microalgae, diatoms, mosses …

The Weekly Challenger, December 18, 2008, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The December 18, 2008, issue of The Weekly Challenger.

Measuring Culture Change As An Evaluation Indicator: Applying Cultural Consensus Analysis To Cultural Models Of Lymphatic Filariasis In Haiti, Kelly M. Simpson

USF Tampa Graduate Theses and Dissertations

Introduction: This project explores the links between shared cultural beliefs in the illness domain, specific to lymphatic filariasis, and a support group program implemented in three Haitian towns. The purpose is to introduce an innovative approach to evaluation, the cultural model evaluation technique (CM Evaluation), as well as gain an understanding of the shifting cognitive belief structure around the cultural domain of lymphatic filariasis in the Haitian setting as associated with a support group intervention.

Method: The sample population was comprised of 241 women across three sites in Haiti: Archaie, Cabaret, and La Plaine. Data were collected from longitudinal surveys …

Access To Health Care Services: A Case Study In Hillsborough County, Florida, Jaime Nodarse

USF Tampa Graduate Theses and Dissertations

The UpliftU® program is a long-term residential program for women and families who are homeless or at risk for homelessness. This program is one part of a larger, community-based non-profit organization serving low-income and homeless families in Hillsborough County, Florida for over 35 years. This program is not an emergency shelter program, but rather offers up to 18 months of participation in a self-sufficiency program to single women and families. The goal of the program is to prevent future homelessness for residents by helping them to reach their highest level of self-sufficiency. After volunteering at this organization for nine months, …

“This Isn’T Like Diphtheria, You Know?”: The Sociocultural Context Of Human Papillomavirus Immunization, Potential Mandates, And Narratives Of Risk Among, Hannah Louise Helmy

USF Tampa Graduate Theses and Dissertations

Many in the biomedical community have praised the recently released Human Papillomavirus (HPV) vaccine, Gardasil, for having the potential to significantly reduce the disease burden of cervical cancer and genital warts. However, complex intersections of ideology, morality, and politics have made this new vaccine considerably contested, particularly as public debate has turned to the ethics of state-mandated HPV vaccination for 11-12 year old girls. Subsequently, the extent to which mandatory vaccinations are accepted by parents and implications regarding the infringement of these coercive measures on their rights to make health care decisions for their children has become powerfully positioned in …

Impact Of Seizure-Related Variables And Psychopathology On Health-Related Quality Of Life In Pediatric Epilepsy, Aja M. Meyer

USF Tampa Graduate Theses and Dissertations

Psychopathology typically is a lasting condition that is persistent from childhood to adulthood. Therefore, it is imperative that children with health conditions and comorbid psychiatric disorders are treated for both conditions as they are likely to have a significant negative impact on children's overall health-related quality of life (HRQL). More specifically, it is important to identify the variables that affect HRQL in children with epilepsy. Research has shown that biomedical variables such as seizure severity and frequency have only moderate relationships with HRQL; therefore, additional factors, such as depression and anxiety, must be identified so that they also may be …

Vectors Of Brevetoxins To Marine Mammals, Leanne J. Flewelling

USF Tampa Graduate Theses and Dissertations

Mass mortalities of Florida manatees (Trichechus manatus latirostris) and bottlenose dolphins (Tursiops truncatus) have been attributed to brevetoxins produced by the Florida red tide dinoflagellate Karenia brevis. The multiple routes through which marine mammals can be exposed to brevetoxins have complicated efforts to understand the mechanisms that lead to mass mortality events. In spring of 2002, 34 endangered Florida manatees died in southwest Florida, and in spring of 2004, 107 bottlenose dolphins died in the Florida Panhandle. These events provided unique opportunities to make clear connections between ingested brevetoxins and marine mammal mortalities without the …

Factors Contributing To The Biofilm-Deficient Phenotype Of Staphylococcus Aureus Sara Mutants, Laura H. Tsang, James E. Cassat, Lindsey N. Shaw, Karen E. Beenken, Mark S. Smeltzer

Molecular Biosciences Faculty Publications

Mutation of sarA in Staphylococcus aureus results in a reduced capacity to form a biofilm, but the mechanistic basis for this remains unknown. Previous transcriptional profiling experiments identified a number of genes that are differentially expressed both in a biofilm and in a sarA mutant. This included genes involved in acid tolerance and the production of nucleolytic and proteolytic exoenzymes. Based on this we generated mutations in alsSD, nuc and sspA in the S. aureus clinical isolate UAMS-1 and its isogenic sarA mutant and assessed the impact on biofilm formation. Because expression of alsSD was increased in a biofilm but …

Usf Women's Club Yearbook, 2008–2009, Usf Women's Club

USF Women’s Club Collection

Yearbook of the USF Women's Club. Inside is a list of past presidents, officers, committee chairmen, and members for 2008–2009. The booklet also includes a tribute to Grace Allen, interest groups, calendar of events, and a copy of the constitution and by-laws from the handbook.

Luminex Microsphere Immunoassay Offers An Improved Method In Testing For Antibodies To Eastern Equine Encephalitis Virus In Sentinel Chicken Sera, Kelly Ann Fitzpatrick

USF Tampa Graduate Theses and Dissertations

Eastern Equine Encephalitis virus has a human mortality rate of 30% of those cases diagnosed, while 30% of those surviving infection remain with neurological sequelae for life (CDC.gov, 2007).

The use of sentinel chickens for surveillance of arboviruses that are known to use birds as a reservoir host, such as St. Louis Encephalitis (SLE), West Nile (WN) virus, Eastern Equine Encephalitis (EEE) and Highlands J (HJ) virus, in Florida began with the Sentinel Chicken Arboviral Surveillance Network in 1978 (Day and Stark, 1996). This network enables the activation of an early warning system for citizens, as well as, county epidemiologists …

Sustainable Control Of Ascaris Lumbricoides (Worms) In A Rural, Disease Endemic And Developing Community: A Systems Approach, Monica Annmarie Gray

USF Tampa Graduate Theses and Dissertations

Parasitic infections, inadequate sanitation, and poor nutrition represent major etiologies that operate in synergy to cause some of the world's most disabling diseases. Citizens of developing nations, especially children living in rural areas, are the most affected. Current research and subsequent interventions have attempted to solve these issues using vertical interventions aimed at minimizing specific health outcomes. This approach does not consider the interaction among causes and the interrelationship between human beings and their environment. Challenges solved in this manner often fail to produce sustainable results or worse, create new problems.

This project proposed the systems approach framework to address …

Human Immunosuppressive Protein, Paul R. Sanberg, Robert W. Engelman, William R. Gower

USF Patents

A method for purifying an immunosuppressant protein (HISP) has the steps of obtaining supernatant from hNT cells; exposing the supernatant to preparative polyacrylamide gel electrophoresis to produce 20 isoelectric fractions, including active isoelectric fraction #10; placing the active isoelectric fraction on a Blue Sepharose column to bind albumin; and collecting the free fraction containing the concentrated, isolated HISP. Also disclosed is a method of treating inflammation, using an effective amount of an HISP. The HISP is anionic, has a molecular weight of 40-100 kDa, an isoelectric point of about 4.8 and is obtained from the supernatant of hNT cells, but …

Etiological Characterization Of Emergency Department Acute Poisoning, Abdmalek S. Khlifi

USF Tampa Graduate Theses and Dissertations

Poisoning is frequently associated with psychological and physiological co-morbidities that can be assessed in order to improve patients' management and reduce cost. The primary objective of this study is to conduct a review of emergency department (ED) poisonings to characterize its demographics and assess associated co-morbidities. The second objective is to explore correlation between personal history of diseases and poisonings. Predictors for poisonings and its outcomes were investigated and risk factors for suicidal poisoning and how it relates to mental illnesses were explored. Six hundred and forty nine cases admitted to ED between 2004 and 2007 were studied. Results indicate …

Implementing A Native Medicinal Plant Garden In Monteverde, Costa Rica, May 2008, Cory Howes

Monteverde Institute: Tropical Ecology and Conservation

The importance of plants has played a powerful role for medicine throughout human history. Tribes in Costa Rica once practiced Shamanism with native flora, but European settlement in the 15th and 16th centuries brought disease, killing much of the aborigines. With their loss, vast amounts of information had been lost regarding what plants they used for medicine. By implementing a garden at the Estación Biológica Monteverde in Monteverde, Costa Rica, an educational tool is created to give students first hand knowledge of 30 native medicinal plants found in Costa Rica. A booklet on the 30 plants was developed to educate …

Dental Hygienists' Beliefs, Norms, Attitudes, And Intentions Toward Treating Hiv/Aids Patients, Barbara Clark-Alexander

USF Tampa Graduate Theses and Dissertations

There is a great demand and need for oral health care during the course of HIV disease (HIV Costs and Services Utilization Study; Marcus et. al., 2005). HIV+ patients identified three key barriers to obtaining oral health treatment: 1) beliefs and attitudes of dental health care providers (DHCPs) may have lead to their unwillingness to treat HIV/AIDS patients; 2) the existence of racial and ethnic disparities in health care in the United States, and 3) how DHCPs perceive their risk of contracting HIV. The fear and stigma associated with treating patients with HIV further compromises their access to care and …

Development Of An Ecological Model To Predict Risk For Acquisition Of Clostridium Difficile-Associated Diarrhea During Acute Care Hospitalization, Susan Elaine Steele

USF Tampa Graduate Theses and Dissertations

Background: The traditional model of infection control has failed to stop the spread of emerging infectious diseases such as Clostridium difficile-associated diarrhea (CDAD) in the acute care environment. Ecological models, which rely upon identification of susceptible hosts, offer an alternative to the prevention of deadly outbreaks. Previous epidemiological research has identified a number of risk factors associated with CDAD. Utilization of this body of research by nurses is limited due to methodological issues that introduce bias and confounding, and use of variables that have limited meaning to the practicing clinical nurse.

Aim: The aim of this study was to …

The Association Between The Measles, Mumps, And Rubella Vaccine And The Development Of Autism: A Meta-Analysis, Rashad Carlton

USF Tampa Graduate Theses and Dissertations

Autism is a childhood developmental disorder characterized by impaired social interaction, difficulty with verbal and nonverbal communication and limited activities. The root cause of autism is unknown. However it has been postulated that administration of the measles, mumps, and rubella (MMR) vaccine may be causally related to the development of autism. MMR vaccination is a requirement for entry into schools, so any increase in adverse events associated with the vaccine carries widespread public health importance.

The primary objective of this study was to conduct a meta-analysis of the association between the MMR vaccination and the development of autism. The meta-analysis …

Multiple Modes Of Mdmx Regulation Affect P53 Activation, Daniele M. Gilkes

USF Tampa Graduate Theses and Dissertations

MDMX has emerged as a negative regulator of p53 transcriptional activity following DNA damage, loss of ribosomal integrity, and aberrant mitogenic signaling. Disruption of rRNA biogenesis by ribosomal stress activates p53 by releasing ribosomal proteins from nucleoli which bind MDM2 and inhibit p53 degradation. We found that p53 activation by ribosomal stress requires degradation of MDMX by MDM2. This occurs by L11 binding to the acidic domain of MDM2 which promotes its E3 ligase function preferentially towards MDMX. Further, unlike DNA damage which regulates MDMX stability through ATM-dependent phosphorylation events, ribosomal stress does not require MDMX phosphorylation suggesting p53 may …

Locating Suitable Habitats For West Nile Virus-Infected Mosquitoes Through Association Of Environmental Characteristics With Infected Mosquito Locations: A Case Study In Shelby County, Tennessee, Esra Ozdenerol, Elzbieta Bialkowska-Jelinska, Gregory N. Taff

School of Geosciences Student Publications

Background: Since its first detection in 2001, West Nile Virus (WNV) poses a significant health risk for residents of Shelby County in Tennessee. This situation forced public health officials to adopt efficient methods for monitoring disease spread and predicting future outbreaks. Analyses that use environmental variables to find suitable habitats for WNV-infected mosquitoes have the potential to support these efforts. Using the Mahalanobis Distance statistic, we identified areas of Shelby County that are ecologically most suitable for sustaining WNV, based on similarity of environmental characteristics to areas where WNV was found. The environmental characteristics in this study were based on …

Nesting Female House Finch Spontaneously Develops Mycoplasma Conjunctivitis, Andrew K. Davis

Oriole

No abstract provided.