Digital Commons Network^™

Identification Of Snps Associated With Multiple Antibiotic Resistance Genes In Eight Clinical Variants Of Enterococcus Faecium., Krishna S. Karia

USF Tampa Graduate Theses and Dissertations

The evolution of multi drug resistant (MDR) bacteria has been well documented these last decade, suggesting that resistance to antibiotics is most commonly associated to single nucleotide polymorphism (SNPs) mutation. To this aim, several molecular methods have been performed to elucidate SNPs in the increasing number of resistant microorganisms. Early detection of MDR bacteria has become of prime necessity in order to combat the disease and ensure public health. Therefore, this research was conducted to screen for SNP mutations correlated to drug resistance in some nosocomial MDR bacteria strains donated from Moffitt Cancer Center (MCC) hospital. Blood, stool, urine, and …

A Micro-Nano Particle System For Sustained Drug Release In Lung Cancer Therapy, Heta N. Jadhav

USF Tampa Graduate Theses and Dissertations

Lung cancer remains the leading cause of cancer-related mortality in men and women worldwide (National Comprehensive Cancer Network). Hence, developing an effective new therapy to treat lung cancer is under intense investigation. Specifically, the sustained release of a drug in lung tumors is critically important. Previous studies at the USF have shown that telmisartan exhibits synergistic properties when combined with Actinomycin-D for lung cancer treatment. The objective of this study is to develop a novel micro/nano system consisting of lipids and chitosan polymers that would be able to deliver Tel directly to the lungs and release its payloads in a …

Trauma Detection Personal Locator Beacon System, Sakshi Sharma

USF Tampa Graduate Theses and Dissertations

This thesis details the development of an automatically-activated personal locator beacon system to facilitate search and rescue of injured users in remote locations. Trauma in the wilderness can result in brain injuries caused by falling from heights, the onset of bad weather condition, cardiological, pulmonary diseased condition, etc. This thesis initially describes the concept of a low-power trauma detection Personal Locator Beacon system and later focuses on detecting the traumatic condition, through four (4) physiological parameters: skin conductance, pulse oximeter, user movement via Freefall and activity/inactivity and impact detection, and finally body temperature. For these four physiological parameters respective sensor …

Use Of High-Probability Instructional Sequences During Pre-Academic Activities For Young Children With Autism Spectrum Disorder, Danielle Russo

USF Tampa Graduate Theses and Dissertations

The antecedent-based strategy, known as the high-probability (high-p) instructional sequence has been one effective way to facilitate teachers to prevent noncompliant behavior and increase acquisition of functional and adaptive behaviors for children with autism spectrum disorders (ASD). However, the outcomes of using the high-p instructional sequence as an intervention in school settings remain inconsistent. In this study, we examined the use of the high-p instructional sequence intervention during pre-academic and academic activities for three young children with ASD. Specifically, the study focused on examining the overall effects of the high-p instructional sequence on initiation and compliance to low-probability (low-p) instructions, …

An Examination Of Changes In Muscle Thickness, Isometric Strength, And Body Water Throughout The Menstrual Cycle, Tayla E. Kuehne

USF Tampa Graduate Theses and Dissertations

Women are often excluded from research studies interested in changes in muscle size and strength due to confounding influence that the menstrual cycle may have on these variables. Although there is considerable data showing that strength may fluctuate throughout the menstrual cycle, the thesis that muscle size changes throughout the cycle is based on the premise that changes in body water throughout the menstrual cycle may influence the size of the muscle. Despite this suggestion there is no experimental data demonstrating that the menstrual cycle has any influence on muscle size. Purpose: The purpose of this study is to examine …

Evaluation Of Native Advertisement Though Third Person Effect Theory: An Experimental Design, Inga Nafetvaridze

USF Tampa Graduate Theses and Dissertations

The main purpose of this research is to determine how people evaluate native advertising in order to figure out how ethical is a native advertising practice. The Third Person Effect Theory was applied to this study. It enables to examine people’s attitude toward native advertising. An experimental design was developed. Participants were exposed to three types of ads to find out if they are able to recognize native ads among other advertisements. The total number of participants was 386. The results were analyzed via ANOVA. The main finding of this study states that individuals perceive the native advertisement in a …

Evaluative Case Study Of Stakeholders’ Perspectives About A Service-Learning Abroad Program, Amela Malkic

USF Tampa Graduate Theses and Dissertations

The present case study evaluated a service-learning study abroad program based on the participants’ perspectives. The stakeholders included undergraduate students, University instructors/coaches, and community partners abroad. The main data sources were semi-structured interviews with volunteer participants and descriptive observations. The purpose of the case study was to identify perceived benefits and perceived challenges resulting from participants’ perspectives and to utilize findings for future program enhancement.

Although the themes of the perceived benefits and challenges varied by stakeholder group and their respective roles in the program, some benefits such as career/professional development and exposure to diversity were shared by multiple stakeholders. …

Enhanced Fluoride Removal In Biosand Filters Using Aluminum Oxide Coated Media And Modified Filter Design, Madison Leigh Rice

USF Tampa Graduate Theses and Dissertations

In 2010, the UN formally stated that access to safe drinking water and sanitation is a human right. Yet, one in three people globally do not have access to safe drinking water. Contaminated drinking water can negatively impact the health of consumers. This is a challenge that disproportionally affects developing regions, where limited access to resources exacerbates the problem. One drinking water contaminant of concern is fluoride. Fluoride is often added in low concentrations to drinking water to enhance consumers’ dental health. However, when water with high concentrations of fluoride is consumed frequently, it can have negative health effects, such …

Choice Versus Reinforcement: Which Produces Better Effects In Decreasing Disruptive Behavior For Students With Or At Risk Of Emotional And Behavioral Disorder?, Sara M. Hordges

USF Tampa Graduate Theses and Dissertations

As the number of students with developmental disabilities increases in schools (U.S. Department of Education, 2017a), so does the need for effective interventions within school settings. Both antecedent and consequence interventions have been conducted within schools in attempts to decrease maladaptive behaviors and increase appropriate behaviors in relation to academics. Providing choices and reinforcement have demonstrated empirical evidence that both interventions were successful in creating positive behavior change in students with emotional and behavioral disorders (EBD). This study compared the use of an antecedent-based intervention (i.e., activity choice) versus a consequence-based intervention (e.g., differential reinforcement without extinction) to determine which …

Some Effects Of Metronome Modality On Speech Rate, Zachary Z. Hand

USF Tampa Graduate Theses and Dissertations

Effects of three metronome modalities on speech rate were examined for three college students. Five conditions were tested for comparison of the effects of each modality. In the first phase of the multielement evaluation none of the modalities succeeded at decreasing the speech rate of the subjects to the target range. During the second phase, a modeling plus feedback component was implemented which successfully decreased the speech rate to the target range for each subject. In the post assessment the effects of the model and feedback component maintained for each subject. These findings may suggest that the use of a …

A Comparison Of Verbal Feedback And Video Feedback To Improve Dance Skills, Shreeya Sudhir Deshmukh

USF Tampa Graduate Theses and Dissertations

Research has shown that video feedback and verbal feedback can improve a variety of skills related to several sports. The purpose of this study was to compare the effectiveness of these two forms of feedback for improving dance skills. In this study, the dance skills of three participants were assessed. Verbal feedback and video feedback were implemented using a multiple baseline across participants with an embedded alternating treatments design to allow for a direct comparison of the interventions. The results indicated that verbal feedback was more effective for one participant and video feedback was more effective for one participant. More …

Care: Collecting And Assessing Cancer Family History To Identify At Risk Individuals Educational Intervention For Community Health Workers, Laura Moreno

USF Tampa Graduate Theses and Dissertations

Background: Collection of family health history (FHH) can identify individuals at increased risk and guide disease-specific recommendations for management, early detection, and prevention. Yet, collection of FHH is often low or infrequent despite national initiatives. In the Hispanic/Latino population, community outreach and education professionals (CORE-Ps) have effectively increased cancer prevention and control behaviors; but, limited research has been conducted to assess genetic and family health history knowledge among CORE-Ps. We sought to evaluate an educational program designed to improve cancer FHH and cancer genetics knowledge, self-efficacy and attitudes. Methods: The ARBOLES Program educates bilingual (Spanish-English) CORE-P to increase knowledge about …

Implementation Of Large-Scale Anaerobic Digestion Of Food Waste At The University Of South Florida, Karamjit Panesar

USF Tampa Graduate Theses and Dissertations

Food waste represents a major sustainability issue in the United States. Food waste represents 18% of landfill space and is the largest contributor of landfill methane emissions. US EPA’s Food Recovery Hierarchy recommends alternatives to landfilling food waste, including source reduction, food donation and anaerobic digestion.

The Student Green Energy Fund (SGEF) Food Recovery Project aims to follow suggestions outlined by the US EPA Food Recovery Hierarchy to encourage the University of South Florida (USF) to become a zero-waste campus. A multi-disciplinary approach aims to source reduce wasted food, look for opportunities for source reduction and anaerobically digest any remaining …

A Multidisciplinary Approach To Trauma Analysis In Cases Of Child Fatality, Jaime D. Sykes

USF Tampa Graduate Theses and Dissertations

Child fatality is an issue of social and forensic significance. Due to the complex nature of these cases, it can be difficult to determine the cause and manner of death (referred to as equivocal death), particularly when differentiating between accidental and inflicted traumatic fatalities. Finite Element Modeling is a tool typically used to elucidate the etiology of fractures. This thesis utilizes a multidisciplinary approach to research the frequency of equivocal deaths among juveniles and the usefulness of FEM as a solution to diagnosing skeletal trauma. The first component examines the problem of misdiagnosed manner of death through retrospective case examinations …

Predictive Validity Of Standards-Based And Curriculum-Embedded Assessments For Predicting Readiness At Kindergarten Entry, Elizabeth Ashton Decamilla

USF Tampa Graduate Theses and Dissertations

As with traditional K-12 educational settings, early childhood assessments have been a primary source of information determining whether early educational experiences have promoted children’s readiness to start school in kindergarten. The level of use of Kindergarten Entry Assessments (KEAs) has become more wide-spread to establish levels of school readiness at kindergarten entry.

This quantitative, correlational study of children in schools that have blended Head Start/Voluntary Prekindergarten funded programs examined the predictive relationships between the independent variables (i.e., VPK Assessments and Teaching Strategies GOLD) and the dependent variable of kindergarten readiness, as measured by the Work Sampling System™ (WSS). Additionally, the …

Enhanced Nitrogen, Organic Matter And Color Removal From Landfill Leachate By Biological Treatment Processes With Biochar And Zeolite, Bisheng Gao

USF Tampa Graduate Theses and Dissertations

Leachate percolating through landfills must be collected and properly treated. However, high concentrations of ammonia (NH4+), refractory organic matter [measured as chemical oxygen demand (COD)], water color and heavy metals in leachate interfere biological processes in conventional wastewater treatment plants. Constructed wetlands (CWs) have been used in the past to treat leachate, but their design and performance has not been properly optimized. In particular, low cost adsorbent materials, such as zeolite and biochar, have the potential to adsorb ammonia and organic compounds, respectively. This increases their retention in the wetland and reduces their toxicity to microorganisms and plants in the …

Evaluating The Use Of Alternative Seating With Children At Risk For Emotional Behavioral Disabilities, Corinne E. Bloom Williams

USF Tampa Graduate Theses and Dissertations

Characteristics of emotional and behavioral disabilities (EBD) include learning difficulties that cannot be explained by intellectual, sensory, or health factors and an inability to build or maintain satisfactory interpersonal relationships with peers and teachers. Often, children with EBD have a tendency to have negative experiences in school and engage in challenging behavior in the classroom. This could stem from their lack of desire or motivation to succeed, which is often found in individuals with EBD. One possible antecedent manipulation, alternative seating, may reduce problem behavior and involves exchanging the typical seating and tables in classrooms for different types of seating …

Pre-Class Physical Activity: Does Choice Expand Its Effect On Disruptive Behavior?, Olivia Mulligan

USF Tampa Graduate Theses and Dissertations

Disruptive behaviors in the classroom have a negative impact on academic engagement and are related to teacher burnout (Abel & Sewell, 1999). Antecedent-based interventions have been shown to be effective in reducing disruptive behaviors (Watson, Timperio, Brown, Best, & Hesketh, 2017) without the need to interrupt academic instruction (Rosenthal-Malek & Mitchell, 1997). Physical activity is an example of an effective antecedent intervention (Lang, et al., 2010), however its efficacy may be related to the type of physical activity (Kern, Koegel, & Dunlap, 1984). The purpose of this study was to evaluate the effect of pre-class physical activity on disruptive classroom …

The Effects Of Perceived Motivations And Mental Distress On The Likelihood Of Reporting And Engaging In Self-Protective Measures Among Victims Of Stalking, Daniela Oramas Mora

USF Tampa Graduate Theses and Dissertations

The current study examines how perceived motivations and mental distress affect decision-making among victims of stalking. Although stalking victimization has become relatively common in recent years, with approximately 1 in 6 women reporting to have been victims of stalking at some point in their lives, victims of stalking are unlikely to report to police compared to victims of other violent crimes (NISVS, 2015). Though studies have explored the dynamics of stalking, little is known about the role of the victim’s perception of the offender’s motivation in the victim's decision to report to the police and engage in self-protective measures. The …

Use Of Pyramidal Training In A Juvenile Residential Facility: Teaching Staff To Self-Monitor Use Of Behavior Specific Praise, Zoe I. Hay

USF Tampa Graduate Theses and Dissertations

Juvenile residential facilities are punitive and restrictive limiting youth opportunity to learn and engage in adaptive behavior. Staff training is necessary to reduce the punishment-based behavior management practices that are often in place and to increase reinforcement of appropriate behavior. Pyramidal training is a cost-effective and efficient strategy to train multiple levels of staff of behavior analytic skills. In this study a pyramidal training approach was used to train juvenile residential level 1 staff to deliver training to level 2 staff, using behavioral skills training (BST) procedures and to implement self-monitoring procedures to improve their practices. A multiple baseline across …

Gofundtransitions: Narratives Of Transnormativity And The Limits Of Crowdfunding Livable Futures, Hayden J. Fulton

USF Tampa Graduate Theses and Dissertations

Crowdfunding websites, such as GoFundMe, have provided a new avenue for (some) individuals to fund their healthcare needs. The use of these sites requires individuals to story their medical need, in the hopes of receiving financial assistance. Trans individuals encounter unique challenges within the healthcare system, including providers’ lack of knowledge, a dearth of competent care, and widespread individual and institutional cissexism. Because of the difficulties acquiring competent trans-healthcare compounded with broader inadequacies in our healthcare system, many trans folks and their loved ones turn to crowdfunding platforms to help cover the cost of medical transition. In this thesis I …

Image-Based Automated Measurement Model To Predict Pelvic Organ Prolapse, Sinan Onal, Susana Karina Lai-Yuen, Paul Bao, Alfredo Weitzenfeld, Stuart Richard Hart

USF Patents

A system and methodology for the automated localization, extraction, and analysis of MRI-based features with clinical information to improve the diagnosis of pelvic organ prolapse (POP). The system can automatically identify reference points for pelvic floor measurements on MRI rapidly and consistent. It provides a prediction model that analyzes the correlation between current and new MRI-based features with clinical information to differentiate patients with and without POP. This system will enable the high throughput analysis of MR images for their correlation with clinical information to better detect POP. The presented system can also be applied to the automated localization and …

Sunitinib-Loaded Mpeg-Pcl Micelles For The Treatment Of Age-Related Macular Degeneration, Jarriaun D. Streets

USF Tampa Graduate Theses and Dissertations

Age-related Macular Degeneration, or AMD will be responsible for the vision impairment of more than 5 million late-aged adults in the next 30 years. Current treatment options include frequent intravitreal injections which report high levels of pain resulting in a low patient compliance. However, there are methods of drug delivery that can decrease the amount of intravitreal injections by sustaining drug release. Polymeric micelles, specifically MPEG-PCL have reported superb biocompatibility and more efficient ways of delivering anti-VEGF drugs such as Sunitinib to the posterior segment of the eye. In this study, the novel micellar formulation exhibited an average DLS particle …

Journey Mapping The Minority Student’S Path Toward Genetic Counseling: A Holistic Picture, Tatiana E. Alvarado-Wing

USF Tampa Graduate Theses and Dissertations

Reasons for limited ethnic and racial diversity among genetic counselors in the United States may be elucidated through better understanding the experiences of minority students who are attending genetic counseling graduate programs as well as recent graduates. Semi-structured interviews were conducted with minority participants using Journey Mapping to elicit touchpoints that positively or negatively impact success at varying points on the path to becoming a genetic counselor. Touchpoints with negative impacts include; late awareness of the profession, observing the lack of diversity in the field, the financial burden of the application process, and microaggressions from peers, program leadership, and clinical …

The Effects Of Feudalism On Medieval English Mobility: A Biological Distance Study Using Nonmetric Cranial Traits., Jonathan H. Barkmeier

USF Tampa Graduate Theses and Dissertations

Social and environmental factors play a key role in determining biocultural phenomena that can be observed on skeletal populations. Genetic markers in the form of nonmetric traits can help understand underlying questions about population movement and subsequent gene flow. During the medieval period in England, feudalism may have limited migration and created sedentary lifestyles for the peasant class who lived and worked on land owned by the nobility. By using a biological distance model, questions about the interactions between rural and urban populations, as well as the restrictive economic system that was in place during the Middle Ages, can be …

Toward Culturally Relevant Emotion Detection Using Physiological Signals, Khadija Zanna

USF Tampa Graduate Theses and Dissertations

Research shows that emotional distress has a statistically significant impact on a student’s grade point average and intent to drop out of college. Because students of different races have varying college experiences, it is important to understand the emotional experiences of different racial groups to better support students’ needs and academic success. In this work, we explore several physiological responses to ten different emotional stimuli captured from 140 students. We employ unsupervised learning via the Density-Based Spatial Clustering of Applications with Noise (DBSCAN) algorithm and supervised learning via Random Forests and Support Vector machines to analyze clustering partitions and classification …

Synthesis Of A Targeted Ph Responsive Nanoparticle For Controlled Release Of Actinomycin D, Alejandro Jesus Gonzalez

USF Tampa Graduate Theses and Dissertations

Out of all cancer diagnosis’s, lung cancer has the highest mortality in both sexes making it the leading cause of cancer deaths. Current therapeutic and clinical methods fail to successfully treat the disease, contributed to the development of multi-drug resistance (MDR). Hence, new therapies are needed that can exploit the tumor microenvironment (TME) to target Cancer Stem Cells (CSC). CSCs constitute a key population of the TME, promoting hypoxia, low intracellular pH, and angiogenesis. Actinomycin D (ACD) is a potent CSC inhibitor, but due to its high toxicity and lack of specificity towards cancer cells presents a need for a …

The Roles Of Emotional Cues And Purchasing Incentives In Wechat Commerce: A Content Analysis, Xuezhu Hao

USF Tampa Graduate Theses and Dissertations

In recent years with the development of social media, a variety of business forms have also developed. In the United States, there are advertising and business forms based on social media platforms such as Facebook and Instagram. However, in China, WeChat is the mainstream social media platform with a staggering number of active online users. As a mainstream social media platform, WeChat has extended a new business form, which is the Micro-business. With the changes in purchasing needs and economic forms, more and more people are participating in Micro-business buying and selling transactions. The formation of a new type of …

Evaluating Effects Of Cancer Genetic Counseling On Several Brief Patient Impact Measures, Alyson Kneusel

USF Tampa Graduate Theses and Dissertations

Many outcomes for assessing cancer genetic counseling (GC) utility have been proposed, with few studies evaluating multiple, theory-based, brief patient-reported experience and outcome measures simultaneously as part of a single study. We conducted a pilot study in which patients seen for pre-test cancer GC took a survey before and after their GC session to evaluate the session’s impact on multiple patient impact measures and assess the relationship between these measures. Measures based on the self-determination theory (SDT) assessed three basic needs including: 1) perceived autonomy support, 2) relatedness to the provider, and 3) competence to make a decision. SDT posits …

Instrumentalization Theory: An Analytical Heuristic For A Heightened Social Awareness Of Machine Learning Algorithms In Social Media, Andrew R. Miller

USF Tampa Graduate Theses and Dissertations

New innovations in information management and communication technologies have produced technological assemblages which have radically altered the way people socialize and interact with the world. The most significant and ubiquitous of these technologies is what is colloquially referred to as ‘machine learning.’ Like most, if not all, technologies, machine learning models are neither wholly good nor bad. Their functional ethics are largely determined by the context in which they are employed. However, their ubiquity demands that we develop a heightened social consciousness of the way machine learning simultaneous constrains, manipulates and democratizes social processes. In order to develop better social …