Digital Commons Network^™

Origin And Epigenetic Regulation Of Cutaneous T Cell Lymphoma, Carly M. Harro

USF Tampa Graduate Theses and Dissertations

The pathogenesis of Cutaneous T cell lymphoma (CTCL) remains unclear. Using conditional knockout mice, we found that ablation of the genomic organizer Special AT-rich sequence-binding protein-1 (Satb1) caused malignant transformation of mature, skin-homing, Notch-activated CD4+ and CD8+ T cells into progressively fatal lymphoma. Mechanistically, Satb1 restrained Stat5 phosphorylation and the expression of skin-homing chemokine receptors in mature T cells. H3K27 and H3K9 trimethylation occluded the SATB1 promoter in Sézary cells, while inhibition of SUV39H1/2 methyltransferases (unlike EZH2 inhibition), restored protective SATB1 expression and selectively abrogated the growth of primary Sézary cells more effectively than romidepsin. Additionally, using single-cell RNA/TCR sequencing …

Thermochemical Conversion Of Lipid-Extracted Algal Biomass To Value-Added Sustainable Products, Magdalini Tsarpali

USF Tampa Graduate Theses and Dissertations

The extensive use of fossil fuels has increased the CO2 emissions, a phenomenon that has increased the demand for renewable energy and resources. Algae, photosynthetic aquatic organisms, have recently caught attention due to their high growth rate and the high CO2 capture ability. Biofuels and bioproducts from algae are considered promising alternatives because of their lower carbon footprint. Lipids are by far the most popular algal product, as they find applications in both fuels, such as biodiesel, and products, such as nutraceuticals and cosmetics. However, after lipid extraction, conversion of the residual algal biomass to value-added products is viewed as …

Passion To Purpose: An Autobiographical Narrative Inquiry Into The Development Of The Summer Ace Program, Owen Hooper

USF Tampa Graduate Theses and Dissertations

Students transitioning from high school to college are met with many challenges beyond academics including social acclimation, campus culture integration, and holistic wellness. Researchers agree that a successful and purposeful transition for incoming college students requires involvement and engagement with peers to help students adapt and increase their likelihood to graduate (Astin, 1999; Kuh, 2009; Tinto, 1998). This autobiographical narrative inquiry reflects on past experiences in assessing the needs of transitioning students at the University of South Florida and developing a purposeful programming model to support students. I aim to help the reader understand my personal commitment to these programs …

Exploring The Relationship Between Student Expected Engagement And Referrals To The Behavioral Intervention Team, Makenzie R. Schiemann

USF Tampa Graduate Theses and Dissertations

Higher education administrators are tasked with supporting and retaining students with increasing needs. These needs often include emotional and mental health issues but can worsen to include suicidality and violence toward others. Traditional campus approaches for supporting students and intervening for violence, such as counseling and campus safety, have been reactionary rather than proactive. Behavioral Intervention Teams (BITs) have emerged as a mechanism for heading off violence before it occurs while also supporting students who may never engage in violence but need support. These teams were born out of the concept that violence is preventable and have grown into a …

Informal Communication, Sensemaking, And Relational Precarity: Constituting Resilience In Remote Work During Covid, Tanya R.M. Vomacka

USF Tampa Graduate Theses and Dissertations

The COVID-19 global pandemic disrupted every corner of the globe, impacting our personal and professional lives with intensity and scope that have yet to be fully comprehended. One such disruption has been to the workplace and organizational culture as businesses, non-governmental agencies, governments, and other organizations worldwide rapidly moved face-to-face operations to remote work. Two years into the pandemic, with vaccines available and the immediate health threat for most healthy individuals waning, businesses still find themselves confronting a changing paradigm as remote work becomes more of a permanent and competitive fixture.

This study explores the impact of remote work on …

Using An Appreciative Inquiry Summit To Improve Support For First Year Experience Community College Instructors, Elise G. Zornow

USF Tampa Graduate Theses and Dissertations

This evaluation study had three main aims: 1) to examine how an Appreciative Inquiry (AI) Summit can be used to promote First Year Experience (FYE) instructors’ sense of autonomy, competence, and relatedness within the context of the FYE program at a community college; 2) to examine how an AI Summit might guide FYE program development; and 3) to examine how and in what ways an AI Summit might influence FYE instructors’ intent to act on the recommendations that result from this approach to program development. Instructors teaching the FYE course at a community college in the Southeastern United States were …

Qualitative Examination Of Sex Therapists' Perspectives Regarding Women With Low Sexual Desire, Tatiana C. Bryan

USF Tampa Graduate Theses and Dissertations

Female sexual desire disorder is often discussed in the literature, but there is a paucity of data on how sex therapists conceptualize and treat this disorder. To address a gap in the current literature, this study collected the perspectives and attitudes of clinicians working with patients presenting with low sexual desire. This qualitative study aimed to understand how sex therapists’ conceptualization of female sexual desire disorder is used to make decisions about diagnosis, treatment, and assessing patient progress. Grounded theory methodology was used to examine the attitudes and beliefs held by sex therapists. Results were used to generate a framework …

On The Reliability Of Wearable Sensors For Assessing Movement Disorder-Related Gait Quality And Imbalance: A Case Study Of Multiple Sclerosis, Steven Díaz Hernández

USF Tampa Graduate Theses and Dissertations

Approximately 33 million American adults had a movement disorder associated with medication use, ear infections, injury, or neurological disorders in 2008, with over 18 million people affected by neurological disorders worldwide. Physical therapists assist people with movement disorders by providing interventions to reduce pain, improve mobility, avoid surgeries, and prevent falls and secondary complications of neurodegenerative disorders. Current gait assessments used by physical therapists, such as the Multiple Sclerosis Walking Scale, provide only semi-quantitative data, and cannot assess walking quality in detail or describe how one’s walking quality changes over time. As a result, quantitative systems have grownas useful tools …

Training Board Certified Behavior Analysts Via Telehealth To Conduct The Trial-Based Functional Analysis, Karie S. John

USF Tampa Graduate Theses and Dissertations

Although many behavior analysts recognize the value of conducting a functional analysis (FA), some report being hesitant to conduct the assessment (Oliver et al., 2015). Two potential barriers to conducting FAs include setting limitations (Roscoe et al., 2015) and lack of trained staff (Deochand et al., 2020). Researchers have addressed these limitations by developing variations of the traditional FA and demonstrating that those procedures could be taught across various populations. Perhaps the issue related to training has less to do with the ability to train FA procedures, and more to do with the accessibility of such trainings. Study 1 addressed …

Establishment Of A Melanoma Esc-Gemm Platform And Its Use To Study Pten Tumor Suppressor Functions, Ilah Bok

USF Tampa Graduate Theses and Dissertations

The cumbersome and time-consuming process of generating new mouse strains and multiallelic experimental animals often hinders the use of genetically engineered mouse models (GEMM) in cancer research. In chapter 2, we describe the development and validation of an embryonic stem cell (ESC)-GEMM platform for rapid modeling of melanoma in mice. The platform incorporates 12 clinically relevant genotypes composed of combinations of four driver alleles (LSL-BrafV600E, LSL-NrasQ61R, PtenFlox, and Cdkn2aFlox) and regulatory alleles to spatiotemporally control the perturbation of genes of interest. The ESCs produce high-contribution chimeras, which recapitulate the melanoma phenotypes of conventionally bred mice. Using the ESC-GEMM platform to …

Defining And Modeling Pericyte-Induced Capillary Network Assembly, Capillary Regression Preceding Pericyte-Stimulated Fibrosis, And Lymphatic Capillary Regression, Scott Stephen Kemp

USF Tampa Graduate Theses and Dissertations

Capillary formation and maturation can only occur through a series of highly orchestrated events that requires the coordinated efforts of both endothelial cells (ECs) and pericytes. While ECs are perfectly capable of creating tube networks on their own, the maturation and stabilization of these tube networks require the presence of a second cell type, pericytes, to create stable capillary networks. Previous studies have identified factors required for pericyte recruitment to EC tube networks, however, we hypothesized that there were still unaccounted for EC-derived molecules that were contributing to pericyte recruitment. In the first part of this study, we used serum-free, …

Targeting The Side-Chain Convergence Of Α-Helical Hot Spots To Design Small-Molecule Mimetics Disrupting Protein-Protein Interaction, Zhen Wang

USF Tampa Graduate Theses and Dissertations

α-Helix accounts for the largest class of protein secondary structures and plays a key role in mediating protein-protein interactions (PPIs). Small-molecule α-helix mimetics mimic the structural features of α-helical hot spots and offer a powerful tool to unravel complex signaling networks and mitigate aberrant PPIs. In this dissertation, the side-chain conformational convergence of α-helical hot spots was disclosed, and insights into the side-chain convergence were exploited to instruct the design of small-molecule α-helix mimetics. The first chapter of this dissertation provides an overview of the characteristics of α-helical hot spots and current strategies to design small-molecule α-helix mimetics. In chapter …

Re-Centering And De-Centering ‘Race’: An Analysis Of Direct-To-Consumer Genetic Testing Organizational Websites, Beatriz Nieto-Fernandez

USF Tampa Graduate Theses and Dissertations

As direct-to-consumer (DTC) genetic testing technologies become an ever-expanding business in the United States, this dissertation project investigates the ways in which two leading organizations, AncestryDNA and 23andMe, utilize discourses of ‘race’ to connect with their customers. This study is particularly significant because it opens up new avenues of research in the field of organizational communication by engaging with a new genre of organizations and the complexities of biocapitalism, in which genetic data is commodified for consumption. Blending computational scraping of data and manual qualitative analysis of organizational texts and videos available on the corporate websites, while using postcolonial studies …

Statistical Monitoring The Quality Of Healthcare Services, Yanqing Kuang

USF Tampa Graduate Theses and Dissertations

In today’s healthcare industry, quality of care is a growing focus in the delivery of healthcare. To improve the quality of care in healthcare delivery, many studies focus on the longterm operational decision making to meet the expectations of healthcare providers and users, such as medical resource allocation, bed planning, staff scheduling, etc. These problems are typically parts of long-term operational decision making, however, time is essential in healthcare system. To ensure the adherence to a high quality of care and detect deterioration in real time, the quality of service should be measured over days or hours instead of just …

Improving Robustness Of Deep Learning Models And Privacy-Preserving Image Denoising, Hadi Zanddizari

USF Tampa Graduate Theses and Dissertations

Applications of deep learning models and convolutional neural networks have been rapidly increased. Although state-of-the-art CNNs provide high accuracy in many applications, recent investigations show that such networks are highly vulnerable to adversarial attacks. The black-box adversarial attack is one type of attack that the attacker does not have any knowledge about the model or the training dataset, but it has some input data set and theirlabels.

In this chapter, we propose a novel approach to generate a black-box attack in a sparse domain, whereas the most critical information of an image can be observed. Our investigation shows that large …

Black Cemeteries Matter: The Erasure Of Historic Black Cemeteries In Polk County, Florida, Juliana C. Waters

USF Tampa Graduate Theses and Dissertations

In the past several years, the Tampa Bay area has experienced a reckoning with regard to the intentional erasure, destruction, and abandonment of historic African American cemeteries such as Zion Cemetery in Tampa or St. Matthews Baptist Church Cemetery in Clearwater. Scholars, journalists, community members, archaeologists, and others have contributed to a growing movement that aims to identify and document these sacred sites in an effort to prevent further destruction. In this vein, this project aimed to identify and record cemeteries in Polk County, examine the processes leading to the erasure of historic Black cemeteries, the history surrounding erasure on …

Development Of Enhanced Algae Strains And Sustainable Cultivation Processes For Production Of Algal Lipids For Biofuels And Bioproducts, Enlin Lo

USF Tampa Graduate Theses and Dissertations

The establishment of an algae industry is crucial for addressing global biofuel and bioproduct demand that meets the Sustainable Development Goals (SDG) worldwide. Photosynthetic microalgae are excellent sources for food, fiber, fuel, feed, and fertilizer, which are the 5Fs of the SDG. However, at present, algae-based materials are not cost-competitive or sufficiently sustainable. Improved productivity, lower cultivation cost, and reduced use of resources are required to transition from lab to industry, necessitating the development of superior strains that have high productivity and can tolerate environmental stress, such as temperature and salinity, so they can be cultivated at large scale outdoors …

Defining The Role Of Oxidized Mitochondrial Dna In Myelodysplastic Syndromes, Grace Anne Ward

USF Tampa Graduate Theses and Dissertations

Myelodysplastic Syndromes (MDS) are heterogeneous bone marrow (BM) failure malignancies characterized by constitutive innate immune activation, NLRP3 inflammasome (IFM) driven pyroptotic cell death, and the induction of interferon-stimulated genes (ISG). MDS often diagnostically resembles other hematologic disorders, as such, additional tools are needed for the discrimination from overlapping disorders. We observed that oxidized mitochondrial DNA (ox-mtDNA) is released upon cytolysis. We hypothesize that levels of ox-mtDNA are high in MDS, allowing us to use it as a diagnostic biomarker; and that cell free ox-mtDNA is contributing to feedforward BM failure.

ROC/AUC analysis demonstrated that ox-mtDNA is a sensitive and specific …

Exploring The Structure And Activity Of Metallo-Tetracyclines, Shahedul Islam

USF Tampa Graduate Theses and Dissertations

Copper as a key component of electron transport chain of eukaryotes is an essential transition metal ion. Copper homeostasis in mammals complex and tightly regulated. Its strong reactivity together with binding with biologically important chemicals can have important repercussions in human health and overall environment. Tetracycline as one of most abundantly used antibiotic of the world has become abundant in the environment as well. Herein in this dissertation we take the journey to explore the complexation of tetracycline with metals in the environment and the intricate interaction between copper and tetracycline by investigating their oxidative behavior once they are complexed. …

Individual Differences In Response To Hostile And Benevolent Sexism In A Stem Interview Context: The Moderating Role Of Behavioral Activation, Elizabeth Kiebel

USF Tampa Graduate Theses and Dissertations

Women continue to face sexism in workplace contexts, especially those that are male dominated, such as science, technology, engineering, and mathematics (STEM). Yet, women often fail to confront the sexism they experience, despite confrontation being an effective way to cope with and prevent future harassment (Fitzgerald et al., 1995; Magley, 2002). To date, no one has assessed the potential moderating role of personality differences related to approach motivation on women’s confrontation of sexism. In this study, women were exposed to either a hostilely sexist or benevolently sexist question during a mock job interview that was purportedly being conducted as part …

Small Heat Shock Protein 22 Preserves Neuronal Function In A Murine Model Of Tauopathy, Santiago Rodriguez Ospina

USF Tampa Graduate Theses and Dissertations

Proteinopathies is a family of diseases associated with the pathological aggregation of protein. There are a multitude of proteinopathies, like tauopathy and synucleinopathy that contribute to neurodegenerative diseases like Alzheimer’s disease (AD), Creutzfeldt-Jakob, Pick’s disease, Frontotemporal dementia, and Parkinson’s disease (PD). Currently, one focus of research in the field is mitigating aggregation-prone proteins contributing to disease state. One method of targeting aggregation is the use of chaperones. Chaperones are molecular machinery that help maintain homeostasis in the cells, through various roles and mechanisms, one of those methods is to regulate protein aggregation. Chaperones can achieve this by affecting protein-protein interactions, …

Oral Narrative Interventions Implemented By Teachers, Speech-Language Pathologists, And Parents, Megan S. Kirby

USF Tampa Graduate Theses and Dissertations

Children’s oral language skills in preschool through early elementary grades can predict reading, writing, and social outcomes ten years into the future. Oral narrative language, which includes storytelling, is a long-established cultural practice in communities around the world. Narratives are the monologic re/telling of a real of fictitious event and people have used them for centuries to entertain, make sense of current and past events, and provide instruction. Oral storytelling does not require physical materials and can be tailored to the cultural and linguistic values of the community in which they are used. Thus, the portability, utility, and ubiquitous nature …

Crow's Nest, March 28, 2022, Crow's Nest Staff

Crow's Nest

The March 28, 2022, issue of the Crow's Nest.

A Mixed Methods Analysis Of Maternal-Infant Feeding Interactions Between Dyads With Opioid Exposure, Kailey T. Rinaldi

USF Tampa Graduate Theses and Dissertations

Infants diagnosed with neonatal opioid withdrawal syndrome (NOWS) exhibit neurobehavioral dysregulation that may make them challenging to feed, potentially impeding positive early maternal-infant interactions. The goal of this study was to investigate dyadic behavior during the maternal-infant feeding interaction, specifically infant dysregulation and maternal sensitivity, between dyads with opioid exposure in the neonatal intensive care unit (NICU). This was a secondary data analysis using a mixed-methods design to analyze videorecorded feeding interactions of mothers with opioid use disorder (OUD) and their infants with NOWS. An integrative literature review investigating maternal sensitivity in mothers with OUD was conducted. Verbalizations expressed by …

Geothermal Development In Kenya: Gender Based Socio-Cultural And Socio-Economic Implications, Faye N. Ricker

USF Tampa Graduate Theses and Dissertations

This dissertation is an ethnographic case study that analyzes the gendered socio-cultural and socio-economic implications for a community that was displaced in order to develop a geothermal energy system in Kenya. The implications are rendered particularly interesting because the displaced community is also one of the communities served by the geothermal power grid. The analysis employs a composite framework of Feminist Political Ecology and the Political Ecology of Climate Change Mitigation. This work explores gendered perceptions and control of energy, livelihood activities, place attachment, and the cultural/spiritual significance of geothermal energy.

Geothermal energy is a potentially promising source of low-carbon …

Medication-Assisted Treatment Versus 12-Step Group Therapy: A Comparative Analysis Of Adherence And Abstinence In Patients With Opioid Use Disorder, Derrick C. Glymph

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation was to do a comparative analysis of the effects of medication-assisted treatment (MAT) and 12-step group therapy on adherence and abstinence in adult patients with opioid use disorder (OUD). The specific aims were (1) to compare treatment adherence and illicit opioid abstinence of adult OUD patients enrolled in MAT and adult OUD patients enrolled in 12-step group therapy at three months and six months after initiation of treatment, and (2) to compare treatment adherence and illicit opioid abstinence of adult OUD patients enrolled in MAT to the treatment adherence and illicit opioid abstinence of adult …

First-Principles-Based Modeling Of Energy Converting Properties Of Conventional And Emerging Ferroelectrics, Maggie Kingsland

USF Tampa Graduate Theses and Dissertations

Ferroelectric materials are a type of multifunctional material that exhibit spontaneous polarization reversable by the application of an electric field. They are used in many technologies such as ferroelectric RAM (FeRAM), piezoelectric devices, RFID chips, and capacitors. However, the most commonly used ferroelectrics are often made with rare, expensive elements and are not environmentally friendly. For example, many prototypical ferroelectrics contain elements such as lead, zirconium, and titanium. As technology grows more advanced, there is a need to discover or manufacture cheaper ferroelectrics and to make them less impactful on human health and the earth as a whole. We set …

Comparing Participants In Discipline And Non-Discipline Specific First-Year Seminars: A Study On Student Characteristics, Expectations, And Changes In Major, Caroline Kaufmann Twachtman

USF Tampa Graduate Theses and Dissertations

First-year seminar (FYS) courses tend to focus on preparing students for successful academic and social integration into the college environment. Many of the studies on FYS courses focus on measuring outcomes such as college grades, retention, and graduation rates. While there is notable documentation of several different types of FYS courses, relatively few studies compare the different types of FYS courses. What, if any, differences might there be in the profiles of students who take either type of course? Is there a certain type of student that enrolls in the discipline-specific course, or a certain type of student that enrolls …

Breast Health Esteem To Motivate Breast Health Behavioral Intentions: An Application Of The Terror Management Health Model, Emily P. Courtney

USF Tampa Graduate Theses and Dissertations

Breast cancer is a pervasive disease affecting millions of people, and a family history of the disease can put individuals at a significantly higher risk of developing breast cancer over the course of one’s lifetime. In turn, women with a family history often perceive themselves as more susceptible to breast cancer. Further, women who have lost family members to breast cancer likely associate the disease itself with death to a greater extent. In addition to this increased risk perception, women with a family history might intertwine breast health with feelings of esteem. It follows that those feelings of esteem should …

Quantifying Functional Performance Of Manual Force Perception And Dynamic Force Control, Benjamin Rigsby

USF Tampa Graduate Theses and Dissertations

This dissertation furthers our understanding of how we (humans) sense and interact withforces, humans, and robots in three distinct cases, which affect robotics and rehabilitation. Chapter 2 examines physical therapists’ (PTs’) consistency when applying assistance forces during rehabilitation. Chapter 3 explores how control performance of an unstable system is affected by the system’s degrees of freedom (DoFs). Chapter 4 investigates the effects of the task speed and direction of perturbing forces on a dynamic task where position is controlled through self-generated forces.

Chapter 2 quantifies the consistency of forces that PTs apply during therapy by using a robotic device. The …