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University of South Florida

KIP Articles

2015

Bats

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Bacteria Isolated From Bats Inhibit The Growth Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Syndrome, Joseph R. Hoty, Tina L. Cheng, Kate E. Langwing Apr 2015

Bacteria Isolated From Bats Inhibit The Growth Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Syndrome, Joseph R. Hoty, Tina L. Cheng, Kate E. Langwing

KIP Articles

Emerging infectious diseases are a key threat to wildlife. Several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. White-nose syndrome, caused by the fungal skin pathogen Pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. The skin microbiome is increasingly understood to play a large role in determining disease outcome. We isolated bacteria from the skin of four bat species, and co-cultured these isolates with P. destructans to identify bacteria that might inhibit or kill P. destructans. We then conducted two …


Trash To Treasure: Assessing Viability Of Wing Biopsies For Use In Bat Genetic Research, Mary Beth Manjerovic, Michelle L. Green, Andrew N. Miller Jan 2015

Trash To Treasure: Assessing Viability Of Wing Biopsies For Use In Bat Genetic Research, Mary Beth Manjerovic, Michelle L. Green, Andrew N. Miller

KIP Articles

The outbreak of white-nose syndrome in North American bats has resulted in massive data collection efforts to characterize the fungus, Pseudogymnoascus destructans. Wing biopsies routinely are collected from live bats, placed in agar media to culture the fungus, and ultimately discarded. We tested whether these discarded tissues represent a viable source of host bat DNA. We found no difference in DNA concentration and no reduction of DNA quality between samples that were extracted immediately compared to samples placed in agar for fungal culture. Although recovered quantities were low, concentrations increased using a cleanup kit. Our study suggests samples collected from …


Range-Wide Genetic Analysis Of Little Brown Bat (Myotis Lucifugus) Populations: Estimating The Risk Of Spread Of White-Nose Syndrome, Maarten J. Vonhof, Amy L. Russell, Cassandra M. Miller-Butterworth Jan 2015

Range-Wide Genetic Analysis Of Little Brown Bat (Myotis Lucifugus) Populations: Estimating The Risk Of Spread Of White-Nose Syndrome, Maarten J. Vonhof, Amy L. Russell, Cassandra M. Miller-Butterworth

KIP Articles

The little brown bat (Myotis lucifugus) is one of the most widespread bat species in North America and is experiencing severe population declines because of an emerging fungal disease, white-nose syndrome (WNS). To manage and conserve this species effectively it is important to understand patterns of gene flow and population connectivity to identify possible barriers to disease transmission. However, little is known about the population genetic structure of little brown bats, and to date, no studies have investigated population structure across their entire range. We examined mitochondrial DNA and nuclear microsatellites in 637 little brown bats (including all currently recognized …


Bacteria Isolated From Bats Inhibit The Growth Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Syndrome Plos One, Joseph R. Hoyt, Tina L. Cheng, Kate E. Langwig Jan 2015

Bacteria Isolated From Bats Inhibit The Growth Of Pseudogymnoascus Destructans, The Causative Agent Of White-Nose Syndrome Plos One, Joseph R. Hoyt, Tina L. Cheng, Kate E. Langwig

KIP Articles

Emerging infectious diseases are a key threat to wildlife. Several fungal skin pathogens have recently emerged and caused widespread mortality in several vertebrate groups, including amphibians, bats, rattlesnakes and humans. White-nose syndrome, caused by the fungal skin pathogen Pseudogymnoascus destructans, threatens several hibernating bat species with extinction and there are few effective treatment strategies. The skin microbiome is increasingly understood to play a large role in determining disease outcome. We isolated bacteria from the skin of four bat species, and co-cultured these isolates with P. destructans to identify bacteria that might inhibit or kill P. destructans. We then conducted two …


Post-White-Nose Syndrome Trends In Virginia’S Cave Bats, 2008-2013, Karen E. Powers, Richard J. Reynolds, Wil Orndorff Jan 2015

Post-White-Nose Syndrome Trends In Virginia’S Cave Bats, 2008-2013, Karen E. Powers, Richard J. Reynolds, Wil Orndorff

KIP Articles

Since its 2009 detection in Virginia hibernacula, the fungal pathogen Pseudogymnoascus destructans causing White-nose Syndrome (WNS) has had a marked impact on cave bats locally. From 2008-2013, we documented numeric and physiologic changes in cave bats through fall swarm (FS), early hibernation (EH), and late hibernation (LH) capture and banding surveys at 18 hibernacula in western Virginia. We coupled active surveys with passive biennial winter counts in 2009, 2011, and 2013. We compared individual body mass index (BMI) across years for FS, EH, and LH hibernation to determine if WNS impacts on extant bats would be manifested by changes in …