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Division Of Research And Economic Development Monthly Report For December 2013, Uri Division Of Research And Economic Development

Reports (Research and Economic Development)

The monthly reports include statistics for project proposals and awards received by individual academic and administrative departments at the University of Rhode Island.

Epitope Recognition In Hla-Dr3 Transgenic Mice Immunized To Tsh-R Protein Or Peptides, Hidefumi Inaba, Leonard Moise, William Martin, Anne S. De Groot, Joe Desrosiers, Ryan Tassone, George Buchman, Takashi Akamizu, Leslie J. De Groot

Institute for Immunology and Informatics Faculty Publications

Development of Graves' disease is related to HLA-DR3. The extracellular domain (ECD) of human TSH receptor (hTSH-R) is a crucial antigen in Graves' disease. hTSH-R peptide 37 (amino acids 78–94) is an important immunogenic peptide in DR3 transgenic mice immunized to hTSH-R. This study examined the epitope recognition in DR3 transgenic mice immunized to hTSH-R protein and evaluated the ability of a mutant hTSH-R peptide to attenuate the immunogenicity of hTSH-R peptide 37. DR3 transgenic mice were immunized to recombinant hTSH-R-ECD protein or peptides. A mutant hTSH-R 37 peptide (ISRIYVSIDATLSQLES: 37m), in which DR3 binding motif position 5 was mutated …

The Two-Faced T Cell Epitope: Examining The Host-Microbe Interface With Janusmatrix, Leonard Moise, Andres H. Gutierrez, Chris Bailey-Kellogg, Frances Terry, Qibin Leng, Karim M. Abdel Hady, Nathan C. Verberkmoes, Marcelo B. Sztein, Phyllis T. Losikoff, William D. Martin, Alan L. Rotham, Anne S. De Groot

Institute for Immunology and Informatics Faculty Publications

Advances in the field of T cell immunology have contributed to the understanding that cross-reactivity is an intrinsic characteristic of the T cell receptor (TCR), and that each TCR can potentially interact with many different T cell epitopes. To better define the potential for TCR cross-reactivity between epitopes derived from the human genome, the human microbiome, and human pathogens, we developed a new immunoinformatics tool, JanusMatrix, that represents an extension of the validated T cell epitope mapping tool, EpiMatrix. Initial explorations, summarized in this synopsis, have uncovered what appear to be important differences in the TCR cross-reactivity of selected regulatory …

Laboratory Assessment Of Beauveria Bassiana (Hypocreales: Clavicipitaceae) Strain Gha For Control Of Listronotus Maculicollis (Coleoptera: Curculionidae) Adults, Christopher Clavet, Emily Hampton, Matthew Requintina, Steven R. Alm

Plant Sciences and Entomology Faculty Publications

Bioassays were designed to evaluate Beauveria bassiana (Balsamo) Vuillemin strain GHA against Listronotus maculicollis (Kirby) adults. B. bassiana and its “inert” carrier oil in the product BotaniGard and the inert carrier oil alone provided 99 and 96% mortality, respectively, in petri dish assays 1 d after treatment when applied in 1 ml water. When the same treatments were applied in 0.5 ml of carrier water, mortality was only 1.4 and 0.7%, respectively, 1 d after treatment. After 10 d in petri dishes, B. bassiana and its inert carrier oil and the inert carrier oil alone applied in 0.5 ml water …

Identification Of Borrelia Burgdorferi Ospc Genotypes In Canine Tissue Following Tick Infestation: Implications For Lyme Disease Vaccine And Diagnostic Assay Design, D. V.L. Rhodes, C. G. Earnhart, Thomas N. Mather, P. F.M. Meeus, R. T. Marconi

Plant Sciences and Entomology Faculty Publications

In endemic regions, Lyme disease is a potential health threat to dogs. Canine Lyme disease manifests with arthritis-induced lameness, anorexia, fever, lethargy, lymphadenopathy and, in some cases, fatal glomerulonephritis. A recent study revealed that the regional mean for the percentage of seropositive dogs in the north-east of the USA is 11.6%. The outer surface protein C (OspC) of Lyme disease spirochetes is an important virulence factor required for the establishment of infection in mammals. It is a leading candidate in human and canine Lyme disease vaccine development efforts. Over 30 distinct ospC phyletic types have been defined. It has been …

Transdisciplinary Case Studies As A Framework For Working In Global Project Teams (2013), Mark W. Rectanus

Online Journal for Global Engineering Education

This article will examine how transdisciplinary case studies can be used to introduce students to critical skills for global project management and intercultural competency. The combination of case study formats, global project management tools, and intercultural communication activities provides frameworks for developing multiple competencies that span professional disciplines and support global internships, service learning projects, or international research assignments. Case studies that draw upon diverse resources and experiential “reports from the field” enable students to successfully prepare for global internships or study abroad and transition from the world of academic study to the complex challenges of everyday life in a …

Research & Innovation For 2012-2013, University Of Rhode Island

Publications (Research and Economic Development)

The 2012-2013 issue of Research & Innovation, a periodical focused on research at the University of Rhode Island.

Medical Apps: Public And Academic Perspectives, William H. Krieger

Philosophy Faculty Publications

Medical apps have featured in popular websites and mainstream news media in recent months. However, there has been almost no mention of these tools in journals focusing on relevant ethical or social issues, including conflict of interest, the role of politics in science, and technological oversight.This essay examines the role that these philosophical issues might play in answering both public and academic questions about these pieces of emergent technology.

Plasma Membrane Compartmentalization Of D2 Dopamine Receptors, Meenakshi Sharma, Jeremy Celver, J. Christopher Octeau, Abraham Kovoor

Biomedical and Pharmaceutical Sciences Faculty Publications

Plasma membrane microcompartments could allow different signaling pathways to operate more efficiently and prevent cross-talk. We utilized a novel in-cell biotin transfer assay to demonstrate that the majority of plasma membrane-expressed D2 dopamine receptor (D2R) is microcompartmentalized within detergent-resistant structures. Conversely, a minority of D2R existed in a detergent-soluble form and interacted in a relatively unrestricted manner with other cellular proteins. The microcompartmentalization of D2R had functional consequences because dopamine-induced internalization of D2R was largely restricted to the compartmentalized receptor. The D2R-containing microcompartments did not correspond to putative detergent-resistant lipid raft structures. First, the detergent-insoluble D2R structures were significantly denser …

Hitting The Right Target: Taxonomic Challenges For, And Of, Plant Invasions, Peter Pyšek, Philip E. Hulme, Laura A. Meyerson, Gideon F. Smith, James S. Boatwright, Neil R. Crouch, Estrela Figueiredo, Llewellyn C. Foxcroft, Vojtěch Jarošik, David M. Richardson, Jan Suda, John R. U. Wilson

Natural Resources Science Faculty Publications

This paper explores how a lack of taxonomic expertise, and by implication a dearth of taxonomic products such as identification tools, has hindered progress in understanding and managing biological invasions. It also explores how the taxonomic endeavour could benefit from studies of invasive species. We review the literature on the current situation in taxonomy with a focus on the challenges of identifying alien plant species and explore how this has affected the study of biological invasions. Biosecurity strategies, legislation dealing with invasive species, quarantine, weed surveillance and monitoring all depend on accurate and rapid identification of non-native taxa. However, such …

Virulence Of Xanthomonas Translucens Pv. Poae Isolated From Poa Annua, Arielle Chaves, Nathaniel Mitkowski

Plant Sciences and Entomology Faculty Publications

Bacterial wilt is a vascular wilt disease caused by Xanthomonas translucens pv. poae that infects Poa annua, a grass that is commonly found on golf course greens throughout the world. Bacterial wilt causes symptoms of etiolation, wilting, and foliar necrosis. The damage is most prevalent during the summer and the pathogen can kill turf under conditions optimal for disease development. Fifteen isolates of X. translucens pv. poae were collected from northern regions in the United States and tested for virulence against P. annua. All 15 isolates were pathogenic on P. annua, but demonstrated variable levels of …

Beyond Humanization And De-Immunization: Tolerization As A Method For Reducing The Immunogenicity Of Biologics, Anne S. De Groot, Frances Terry, Leslie Cousens, William Martin

Institute for Immunology and Informatics Faculty Publications

Immune responses to some monoclonal antibodies (mAbs) and biologic proteins interfere with their efficacy due to the development of anti-drug antibodies (ADA). In the case of mAbs, most ADA target ‘foreign’ sequences present in the complementarity determining regions (CDRs). Humanization of the mAb sequence is one approach that has been used to render biologics less foreign to the human immune system. However, fully human mAbs can also drive immunogenicity. De-immunization (removing epitopes) has been used to reduce biologic protein immunogenicity. Here, we discuss a third approach to reducing the immunogenicity of biologics: introduction of Treg epitopes that stimulate …

Immunopathogenesis Versus Protection In Dengue Virus Infections, Alan L. Rothman, Carey L. Medin, Heather Friberg, Jeffery R. Currier

Institute for Immunology and Informatics Faculty Publications

Dengue viruses (DENV) are mosquito-borne viruses that cause significant morbidity. The existence of four serotypes of DENV with partial immunologic cross-reactivity creates the opportunity for individuals to experience multiple acute DENV infections over the course of their lifetimes. Research over the past several years has revealed complex interactions between DENV and the human innate and adaptive immune systems that can have either beneficial or detrimental influences on the outcome of infection. Further studies that seek to distinguish protective from pathological immune responses in the context of natural DENV infection as well as clinical trials of candidate DENV vaccines have an …

Tregitope Peptides: The Active Pharmaceutical Ingredient Of Ivig?, Anne S. De Groot, Leslie Cousens, Federico Mingozzi, William Martin

Institute for Immunology and Informatics Faculty Publications

Five years ago, we reported the identification and characterization of several regulatory T-cell epitopes (now called Tregitopes) that were discovered in the heavy and light chains of IgG (De Groot et al. Blood, 2008). When added ex vivo to human PBMCs, these Tregitopes activated regulatory T cells (Tregs), increased expression of the transcription factor FoxP3, and induced IL- 10 expression in CD4+ T cells. We have now shown that coadministration of the Tregitopes in vivo, in a number of different murine models of autoimmune disease, can suppress immune responses to antigen in an antigen-specific manner, and that this response is …

Report From The Field - Overview Of The Sixth Annual Vaccine Renaissance Conference, Denice Spero, Lauren Levitz, Anne S. De Groot

Institute for Immunology and Informatics Faculty Publications

The Sixth Annual Vaccine Renaissance Conference, hosted by the Institute for Immunology and Informatics (iCubed) at the University of Rhode Island (URI), took place on October 15–17, 2012. This conference provides a forum for the review of current progress in the discovery and development of vaccines, and creates an environment for the exchange of ideas. Dr. Joel McCleary opened the conference with a warning about the importance of preparing for well-defined biowarfare threats, including tularemia and Staphylococcal enterotoxin B. Following the keynote address, sessions explored biodefense and preparation for pandemic and biowarfare threats; vaccines for emerging and re-emerging neglected …

Low Immunogenicity Predicted For Emerging Avian-Origin H7n9, Anne S. De Groot, Matthew Ardito, Frances Terry, Lauren Levitz, Ted Ross, Leonard Moise, William Martin

Institute for Immunology and Informatics Faculty Publications

A new avian-origin influenza virus emerged near Shanghai in February 2013, and by the beginning of May it had caused over 130 human infections and 36 deaths. Human-to-human transmission of avian-origin H7N9 influenza A has been limited to a few family clusters, but the high mortality rate (27%) associated with human infection has raised concern about the potential for this virus to become a significant human pathogen. European, American, and Asian vaccine companies have already initiated the process of cloning H7 antigens such as hemagglutinin (HA) into standardized vaccine production vehicles. Unfortunately, previous H7 HA-containing vaccines have been poorly immunogenic. …

Highly Efficient Quenching Of Nanoparticles For The Detection Of Electron-Deficient Nitroaromatics, Patrick Marks, Sage Cohen, Mindy Levine

Chemistry Faculty Publications

Reported herein is the highly efficient quenching of fluorescent organic nanoparticles by 2,4-dinitrotoluene (DNT) and 2,4,6-trinitrotoluene (TNT). These fluorescent nanoparticles are formed from the hydrophobic collapse of fluorescent polymer chains, and display quenching efficiencies that are in line with the highest reported literature values. Moreover, the fluorescent quenching occurs only for the fluorescent nanoparticles, and not for the precursor polymer solutions, which display marked insensitivity to the presence of nitroaromatics. This aggregation-dependent fluorescent quenching has numerous applications for the detection of small-molecule electron-deficient analytes.

Wellness Incentives: Can The Use Of Financial Incentives And Penalties Be Effective For Motivating Participation In Wellness Programs And Sustaining Health Behavior Modification?, Donna Moschetti

Seminar Research Paper Series

Employer-based health promotion programs have been around for about four decades, but in recent years increasing attention is being given to the use of financial incentives and penalties as ways to encourage employees to become more accountable for their own health. The use of wellness incentives has been gaining traction, and employer-sponsored programs that incorporate these types of strategies have been growing rapidly. With the passage of the Affordable Care Act, the upcoming change to federal rules on wellness incentives is likely to drive up participation rates. However, this is not without controversy and it has raised some very important …

An Escherichia Coli Nissle 1917 Missense Mutant Colonizes The Streptomycin-Treated Mouse Intestine Better Than The Wild Type But Is Not A Better Probiotic, Jimmy Adediran, Mary P. Leatham-Jensen, Matthew E. Mokszycki, Jakob Frimodt-Møller, Karen A. Krogfelt, Krystyna Kazmierczak, Linda J. Kenney, Tyrrell Conway, Paul S. Cohen

Cell and Molecular Biology Faculty Publications

Previously we reported that the streptomycin-treated mouse intestine selected for two different Escherichia coli MG1655 mutants with improved colonizing ability: nonmotile E. coli MG1655 flhDC deletion mutants that grew 15% faster in vitro in mouse cecal mucus and motile E. coli MG1655 envZ missense mutants that grew slower in vitro in mouse cecal mucus yet were able to cocolonize with the faster-growing flhDC mutants. The E. coli MG1655 envZ gene encodes a histidine kinase that is a member of the envZ-ompR two-component signal transduction system, which regulates outer membrane protein profiles. In the present investigation, the envZ _P41L gene was …

Cyclodextrin-Enhanced Extraction And Energy Transfer Of Carcinogens In Complex Oil Environments, Nicole Serio, Chitapom Chanthalyma, Lindsey Prignano, Mindy Levine

Chemistry Faculty Publications

Reported herein is the use of γ-cyclodextrin for two tandem functions: (a) the extraction of carcinogenic polycyclic aromatic hydrocarbons (PAHs) from oil samples into aqueous solution, and (b) the promotion of highly efficient energy transfer from the newly extracted PAHs to a high-quantum yield fluorophore. The extraction proceeded in moderate to good efficiencies, and the resulting cyclodextrin-promoted energy transfer lead to a new, brightly fluorescent signal in the aqueous solution. The resulting dual-function system (extraction followed by energy transfer) has significant relevance in the environmental detection and cleanup of oil spill-related carcinogens.

Synthesis Of A Fluorescent Conjugated Polymer In The Undergraduate Organic Teaching Laboratory, Teresa Mako, Mindy Levine

Chemistry Faculty Publications

Fluorescent organic polymers are used in a wide range of applications, including explosive sensing, clean energy technologies, and biological detection schemes. However, such polymers are rarely synthesized in an undergraduate organic teaching laboratory, due to their air and moisture sensitivity. The synthesis of a fluorescent organic polymer, poly[2-methoxy-5-(2-ethylhexyloxy)-1,4-phenylenevinylene] (MEH-PPV), is described for the upper-division undergraduate organic laboratory. This polymerization is accomplished using straightforward procedures to exclude air and moisture, without requiring the use of a glovebox or strict inert-atmosphere conditions. Once synthesized, the polymer is used for two applications, the fabrication of fluorescent thin films and the formation of organic …

Ethnicity As A Moderator Of Motivational Interviewing For Incarcerated Adolescents After Release, Mary Clair, L.A.R. Stein, Shayna Soenksen, Rosemarie A. Martin, Rebecca Lebeau, Charles Golembeske

Psychology Faculty Publications

Motivational interviewing (MI) has been found to be an effective treatment for substance using populations, including incarcerated adolescents. Although some studies suggest MI is more successful with individuals from minority backgrounds, the research remains mixed. The current study investigated the impact of ethnicity on treatment in reducing alcohol and marijuana use among incarcerated adolescents. Adolescents (14–19 years of age) were recruited from a state juvenile correctional facility and randomly assigned to receive MI or relaxation therapy (RT) (N = 147; 48 White, 51 Hispanic, and 48 African American; 126 male; 21 female). Interviews were conducted at admission to the …

Abcc3 As A Marker For Multidrug Resistance In Non-Small Cell Lung Cancer, Yanbin Zhao, Hailing Lu, An Yan, Yanmei Yang, Qingwei Meng, Lichun Sun, Hui Pang, Chunhong Li, Xiaoqun Dong, Li Cai

Biomedical and Pharmaceutical Sciences Faculty Publications

Multidrug resistance (MDR) contributes to the failure of chemotherapy and high mortality in non-small cell lung cancer (NSCLC). We aim to identify MDR genes that predict tumor response to chemotherapy. 199 NSCLC fresh tissue samples were tested for chemosensitivity by MTT assay. cDNA microarray was done with 5 samples with highest resistance and 6 samples with highest sensitivity. Expression of ABCC3 mRNA and protein was detected by real-time PCR and immunohistochemisty, respectively. The association between gene expression and overall survival (OS) was examined using Cox proportional hazard regression. 44 genes were upregulated and 168 downregulated in the chemotherapy-resistant group. ABCC3 …

Insights Into The Conformation Of Aminofluorene-Deoxyguanine Adduct In A Dna Polymerase Active Site, Vaidyanathan G. Vaidyanathan, Fengting Liang, William A. Beard, David D. Shock, Samuel H. Wilson, Bongsup P. Cho

Biomedical and Pharmaceutical Sciences Faculty Publications

The active site conformation of the mutagenic fluoroaminofluorene-deoxyguanine adduct (dG-FAF, N-(2′-deoxyguanosin-8-yl)-7-fluoro-2-aminofluorene) has been investigated in the presence of Klenow fragment of Escherichia coli DNA polymerase I (Kfexo⁻) and DNA polymerase β (pol β) using ¹⁹F NMR, insertion assay, and surface plasmon resonance. In a single nucleotide gap, the dG-FAF adduct adopts both a major-groove- oriented and base-displaced stacked conformation, and this heterogeneity is retained upon binding pol β. The addition of a non-hydrolysable 2′-deoxycytosine-5′-[(α,β)-methyleno]triphosphate (dCMPcPP) nucleotide analog to the binary complex results in an increase of the major groove conformation of the adduct at the expense …

Synthesis And Antiproliferative Activities Of Quebecol And Its Analogs, Kasiviswanadharaju Pericherla, Amir Nasrolahi Shirazi, V. Kameshwara Rao, Rakesh K. Tiwari, Nicholas Dasilva, Kellen T. Mccaffrey, Yousef A. Beni, Antonion González-Sarrías, Navindra P. Seeram, Keykavous Parang, Anil Kumar

Biomedical and Pharmaceutical Sciences Faculty Publications

Simple and efficient synthesis of quebecol and a number of its analogs was accomplished in five steps. The synthesized compounds were evaluated for antiproliferative activities against human cervix adenocarcinoma (HeLa), human ovarian carcinoma (SK-OV-3), human colon carcinoma (HT-29), and human breast adenocarcinoma (MCF-7) cancer cell lines. Among all the compounds, 7c, 7d, 7f, and 8f exhibited antiproliferative activities against four tested cell lines with inhibition over 80% at 75 μM after 72 h, whereas, compound 7b and 7g were more selective towards MCF-7 cell line. The IC₅₀ values for compounds 7c, 7d, and 7f …

A Comparative Study Of Hollow Copper Sulfide Nanoparticles And Hollow Gold Nanospheres On Degradability And Toxicity, Laingran Guo, Irene Panderi, Daisy D. Yan, Kevin Szulak, Yajuan Li, Yi-Tzai Chen, Hang Ma, Daniel B. Niesen, Navindra P. Seeram, Aftab Ahmed, Bingfang Yan, Dionysios Pantazatos, Wei Lu

Biomedical and Pharmaceutical Sciences Faculty Publications

Gold and copper nanoparticles have been widely investigated for photothermal therapy of cancer. However, degradability and toxicity of these nanoparticles remain concerns. Here, we compare hollow CuS nanoparticles (HCuSNPs) with hollow gold nanospheres (HAuNS) in similar particle sizes and morphology following intravenous administration to mice. The injected pegylated HCuSNPs (PEG-HCuSNPs) are eliminated through both hepatobiliary (67 percentage of injected dose, %ID) and renal (23 %ID) excretion within one month postinjection. By contrast, 3.98 %ID of Au is excreted from liver and kidney within one month after iv injection of pegylated HAuNS (PEG-HAuNS). Comparatively, PEG-HAuNS are almost nonmetabolizable, while PEG-HCuSNPs are …

Caloric Restriction-Mediated Induction Of Lipid Metabolism Gene Expression In Liver Is Enhanced By Keap1-Knockdown, Supriya R. Kullkarni, Laura E. Armstrong, Angela L. Slitt

Biomedical and Pharmaceutical Sciences Faculty Publications

Purpose: CR increases fatty acid oxidation to decrease tissue lipid content. The Nuclear factor E2-related factor 2 (Nrf2)-Kelch like ECH associated Protein 1 (Keap1) pathway is an antioxidant gene regulatory pathway that has been previously investigated in weight gain. However, limited interaction of Nrf2/Keap1 and CR exists. The purpose of this study was to determine how Keap1 knockdown (Keap1-KD), which is known to increase Nrf2 activity, affects the CR response, such as weight loss, hepatic lipid decrease, and induction of fatty acid oxidation gene expression.

Methods: C57BL/6 and Keap1-KD mice were maintained on 40% CR or fed ad libitum for …

H-Ns Is A Negative Regulator Of The Two Hemolysin/Cytotoxin Gene Clusters In Vibrio Anguillarum, Xiangyu Mou, Edward J. Spinard, Maureen V. Driscoll, Weinjing Zhao, David R. Nelson

Cell and Molecular Biology Faculty Publications

Hemolysins produced by Vibrio anguillarum have been implicated in the development of hemorrhagic septicemia during vibriosis, a fatal fish disease. Previously, two hemolysin gene clusters responsible for the hemolysis and cytotoxicity of V. anguillarum were identified: the vah1-plp gene cluster and the rtxACHBDE gene cluster. In this study, we identified the hns gene, which encodes the H-NS protein and acts as a negative regulator of both gene clusters. The V. anguillarum H-NS protein shares strong homology with other bacterial H-NS proteins. An hns mutant exhibited increased hemolytic activity and cytotoxicity compared to the wild-type strain. Complementation of the hns mutation …

Cyclic Peptides Containing Tryptophan And Arginine As Src Kinase Inhibitors, Amir Nasrolahi Shirazi, Rakesh Kumar Tiwari, Alex Brown, Dindyal Mandal, Gongqin Sun, Keykavous Parang

Cell and Molecular Biology Faculty Publications

A number of cyclic and linear peptides containing various combinations of amino acids were evaluated for their Src kinase inhibitory potency. Among all the peptides, cyclic decapeptide C[RW]₅ containing alternative arginine (R) and tryptophan (W) residues was found to be the most potent Src kinase inhibitor. C[RW]₅ showed higher inhibitory activity (IC₅₀ = 2.8 μM) than C[KW]₅, L(KW)₅, C[RW]₄, and C[RW]₃ with IC₅₀ values of 46.9, 69.1, 21.5, and 25.0 μM, respectively, as determined in a fluorescence intensity-based assay. Thus, the cyclic nature, the presence of arginine, ring size, …

Synthesis And Evaluation Of Cytotoxic Activity Of Substituted N-(9-Oxo-9h-Xanthen-4-Yl)Benzenesulfonamides, Somayeh Motavallizadeh, Asal Asal Fallah-Taftia, Saeedeh Maleki, Amir Nasrolahi Shirazi, Mahboobeh Pordeli, Maliheh Safavi, Sussan Kabudanian Ardestani, Shaaban Asd, Rakesh Tiwari, Donghoon Oh, Abbas Shafiee, Alireza Foroumadi, Keykavous Parang, Tahmineh Akbarzadeh

Biomedical and Pharmaceutical Sciences Faculty Publications

Several novel N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamides derivatives were prepared as potential antiproliferative agents. The in vitro antiproliferative activity of the synthesized compounds was investigated against a panel of tumor cell lines including breast cancer cell lines (MDA-MB-231, T-47D) and neuroblastoma cell line (SK-N-MC) using MTT colorimetric assay. Etoposide, a well-known anticancer drug, was used as a positive standard drug. Among synthesized compounds, 4-methoxy-N-(9-oxo-9H-xanthen-4-yl)benzenesulfonamide (5i) showed the highest antiproliferative activity against MDA-MB-231, T-47D, and SK-N-MC cells. Furthermore, pentafluoro derivatives 5a and 6a exhibited higher antiproliferative activity than doxorubicin against human leukemia cell line (CCRF-CEM) and breast adenocarcinoma …