Digital Commons Network^™

Biological Evaluation Of Dna Biomarkers In A Chemically Defined And Site-Specific Manner, Ke Bian, James C. Delaney, Xianhao Zhou, Deyu Li

Biomedical and Pharmaceutical Sciences Faculty Publications

As described elsewhere in this Special Issue on biomarkers, much progress has been made in the detection of modified DNA within organisms at endogenous and exogenous levels of exposure to chemical species, including putative carcinogens and chemotherapeutic agents. Advances in the detection of damaged or unnatural bases have been able to provide correlations to support or refute hypotheses between the level of exposure to oxidative, alkylative, and other stresses, and the resulting DNA damage (lesion formation). However, such stresses can form a plethora of modified nucleobases, and it is therefore difficult to determine the individual contribution of a particular modification …

Evaluating Native American Bird Use And Bird Assemblage Variability Along The Oregon Coast, Kristine M. Bovy, Madonna L. Moss, Jessica E. Watson, Frances J. White, Timothy T. Jones, Heather A. Ulrich, Julia K. Parrish

Sociology and Anthropology Faculty Publications

Native American use of birds on the Oregon coast is not well known and has never been synthesized to present a regional understanding. We rectify this by analyzing data from 26 zooarchaeological assemblages, including three previously unpublished bird assemblages: Umpqua/Eden (35DO83), Whale Cove (35LNC60), and the Dunes Site (35CLT27). We employ a series of non-parametric randomization tests to directly evaluate patterns of taxonomic diversity, correlations with nearby breeding colonies, and broader procurement strategies discussed in ethnohistorical accounts. We compare the assemblages to contemporary surveys of naturally beached birds as observed by COASST (Coastal Observation Seabird Survey Team) and evaluate whether …

Extreme Site Fidelity As An Optimal Strategy In An Unpredictable And Homogeneous Environment, Brian D. Gerber, Mevin B. Hooten, Christopher P. Peck, Mindy B. Rice, James H. Gammonley, Anthony D. Apa, Amy J. Davis

Natural Resources Science Faculty Publications

1.Animal site fidelity structures space‐use, population demography, and ultimately gene flow. Understanding the adaptive selection for site fidelity patterns provides a mechanistic understanding to both spatial and population processes. This can be achieved by linking space‐use with environmental variability (spatial and temporal) and demographic parameters. However, rarely is the environmental context that drives the selection for site fidelity behavior fully considered.

2.We use ecological theory to understand whether the spatial and temporal variability in breeding site quality can explain the site fidelity behavior and demographic patterns of Gunnison sage‐grouse (Centrocercus minimus). We examined female site fidelity patterns across …

The Importance Of Isotopic Turnover For Understanding Key Aspects Of Animal Ecology And Nutrition, Wales A. Carter, Ulf Bauchinger, Scott R. Mcwilliams

Natural Resources Science Faculty Publications

Stable isotope-based methods have proved to be immensely valuable for ecological studies ranging in focus from animal movements to species interactions and community structure. Nevertheless, the use of these methods is dependent on assumptions about the incorporation and turnover of isotopes within animal tissues, which are oftentimes not explicitly acknowledged and vetted. Thus, the purpose of this review is to provide an overview of the estimation of stable isotope turnover rates in animals, and to highlight the importance of these estimates for ecological studies in terrestrial, freshwater, and marine systems that may use a wide range of stable isotopes. Specifically, …

Assessment Of Night-Time Lighting For Global Terrestrial Protected And Wilderness Areas, Liangxian Fan, Jianjun Zhao, Yeqiao Wang, Zhoupeng Ren, Hongyan Zhang, Xiaoyi Guo

Natural Resources Science Faculty Publications

Protected areas (PAs) play an important role in biodiversity conservation and ecosystem integrity. However, human development has threatened and affected the function and effectiveness of PAs. The Defense Meteorological Satellite Program/Operational Linescan System (DMSP/OLS) night-time stable light (NTL) data have proven to be an effective indicator of the intensity and change of human-induced urban development over a long time span and at a larger spatial scale. We used the NTL data from 1992 to 2013 to characterize the human-induced urban development and studied the spatial and temporal variation of the NTL of global terrestrial PAs. We selected seven types of …

Independence Day: Post-Fledging Movements And Behavior Of Adult Eastern Towhees (Pipilo Erythrophthalmus) In Landscapes Managed For American Woodcock (Scolopax Minor), Stephen J. Brenner, Scott R. Mcwilliams

Natural Resources Science Faculty Publications

Umbrella species management offers a potential solution to the financial and logistical challenges of managing for the many declining species in early-successional forests, a habitat that is also critical for many mature and young forest songbird species during the post-fledging and post-breeding period. We investigated the movements of adult Eastern Towhees (Pipilo erythrophthalmus) during the post-fledging period in 4 km² landscapes managed for American Woodcock (Scolopax minor), a popular umbrella species candidate for young forest management. Home range size (mean = 2.8 ha, SE 0.33) did not differ during the post-fledging period between adult towhees …

Modeling Spatiotemporal Abundance Of Mobile Wildlife In Highly Variable Environments Using Boosted Gamlss Hurdle Models, Adam Smith, Benjamin Hofner, Juliet S. Lamb, Jason Osenkowski, Taber Allison, Giancarlo Sadoti, Scott R. Mcwilliams, Peter W. C. Paton

Natural Resources Science Faculty Publications

Modeling organism distributions from survey data involves numerous statistical challenges, including accounting for zero‐inflation, overdispersion, and selection and incorporation of environmental covariates. In environments with high spatial and temporal variability, addressing these challenges often requires numerous assumptions regarding organism distributions and their relationships to biophysical features. These assumptions may limit the resolution or accuracy of predictions resulting from survey‐based distribution models. We propose an iterative modeling approach that incorporates a negative binomial hurdle, followed by modeling of the relationship of organism distribution and abundance to environmental covariates using generalized additive models (GAM) and generalized additive models for location, scale, and …

Habitat Selection Of American Woodcock And Its Implications For Habitat Management Where Young Forests Are Rare, Roger J. Masse, Brian C. Tefft, Bill Buffum, Scott R. Mcwilliams

Natural Resources Science Faculty Publications

American woodcock (Scolopax minor; hereafter woodcock) habitat use or selection has been studied extensively since the mid-1960s; most such studies, however, have taken place when and where young forest habitat selected by woodcock was relatively common. Woodcock population declines have been mostly attributed to loss of young forest vegetation types throughout the species’ range. Thus, understanding woodcock habitat selection and the benefits of habitat management in areas where young forests are rare is important in conserving woodcock and other wildlife that uses young forest. We conducted studies of male radio-tagged woodcock in Rhode Island, USA, when and where the extent …

Active Learning Approaches To Teaching Soil Science At The College Level, José A. Amador

Natural Resources Science Faculty Publications

Traditional passive approaches to teaching, such as lectures, are not particularly effective at promoting student learning, or at developing the qualities that employers seek in graduates from soil science programs, such as problem-solving and critical thinking skills. In contrast, active learning approaches have been shown to promote these very qualities in students. Here, I discuss my use of active learning approaches to teach soil science at the introductory and advanced levels, with particular focus on problem-based learning (PBL), and combined just-in-time teaching (JITT) and peer instruction (PI). A brief description of the each pedagogical approach is followed by evidence of …

Exposure To A Slightly Sweet Lipid-Based Nutrient Supplement During Early Life Does Not Increase The Level Of Sweet Taste Most Preferred Among 4- To 6-Year-Old Ghanaian Children: Follow-Up Of A Randomized Controlled Trial, Harriet Okronipa, Mary Arimond, Charles D. Arnold, Rebecca R. Young, Seth Adu-Afarwuah, Solace M. Tamakloe, Maku E. Ocansey, Sika M. Kumordzie, Brietta M. Oaks, Julie A. Mennella, Kathryn G. Dewey

Nutrition Faculty Publications

Background: The impact of feeding a slightly sweet nutrient supplement early in life on later sweet taste preference is unknown.

Objective: We tested the hypothesis that the level of sucrose most preferred by 4–6-y-old children exposed to a slightly sweet lipid-based nutrient supplement (LNS) early in life would not be higher than that of children never exposed to LNS.

Design: We followed up children born to women (n = 1,320) who participated in a randomized trial in Ghana. In one group, LNS was provided to women on a daily basis during pregnancy and the first 6 …

Prenatal And Postnatal Lipid-Based Nutrient Supplementation And Cognitive, Social-Emotional, And Motor Function In Preschool-Aged Children In Ghana: A Follow-Up Of A Randomized Controlled Trial, Maku E. Ocansey, Seth Adu-Afrawuah, Sika M. Kumordzie, Harriet Okronipa, Rebecca R. Young, Solace M. Tamakloe, Brietta M. Oaks, Kathryn G. Dewey, Elizabeth L. Prado

Nutrition Faculty Publications

Background: Adequate nutrition is necessary for brain development during pregnancy and infancy. Few randomized controlled trials of supplementation during these periods have measured later developmental outcomes.

Objective: Our objective was to investigate the effects of provision of prenatal and postnatal lipid-based nutrient supplements (LNS) on child development at preschool age.

Methods: We conducted a follow-up study of 966 children aged 4–6 y in 2016, born to women who participated in the International Lipid-Based Nutrient Supplements-DYAD trial conducted in Ghana in 2009–2014, representing 79% of eligible children. Women ≤20 weeks of gestation were randomized to daily LNS or …

Sex Differences In Lifestyle Behaviors Among U.S. College Freshmen, Melissa D. Olfert, Makenzie L. Barr, Camille C. Charlier, Geoffrey W. Greene, Wenjun Zhou, Sarah E. Colby

Nutrition Faculty Publications

Within lifestyle behavior research, the sex of populations causes differences in behaviors and outcomes of studies. This cross-sectional study investigated lifestyle behavior patterns in college students, examining sex differences in four areas: Nutrition, physical activity, sleep, and stress. Data from over 1100 college freshmen across 8 United States universities were used for this cross-sectional analysis. Self-reported data assessed fruit and vegetable intake, fat percent intake, physical activity, perceived stress, and sleep quality. Statistical analysis included Pearson chi-squared and Mann–Whitney’s U tests for scores by sex. Likewise, healthy cut-offs were used to determine frequency of participants within range of the five …

Global Dynamics Of Perturbation Of Certain Rational Difference Equation, Sabina Hrustić, Mustafa R.S. Kulenović, Samra Moranjkić, Zehra Nurkanović

Mathematics Faculty Publications

We investigate the global asymptotic stability of the difference equation of the form x n+1 = Ax n2 + F/ax n2 + ex n-1 ; n = 0, 1, . . ., with positive parameters and nonnegative initial conditions such that x 0 +x -1 > 0. The map associated to this equation is always decreasing in the second variable and can be either increasing or decreasing in the first variable depending on the parametric space. In some cases, we prove that local asymptotic stability of the unique equilibrium point implies global asymptotic stability.

Colorimetric Detection Of Aliphatic Alcohols In Β-Cyclodextrin Solutions, Anna Haynes, Priva Halpert, Mindy Levine

Chemistry Faculty Publications

The sensitive, selective, and practical detection of aliphatic alcohols is a continuing technical challenge with significant impact in public health research and environmental remediation efforts. Reported herein is the use of a β-cyclodextrin derivative to promote proximity-induced interactions between aliphatic alcohol analytes and a brightly colored organic dye, which resulted in highly analyte-specific color changes that enabled accurate alcohol identification. Linear discriminant analysis of the color changes enabled 100% differentiation of the colorimetric signals obtained from methanol, ethanol, and isopropanol in combination with BODIPY and Rhodamine dyes. The resulting solution-state detection system has significant broad-based applicability because it uses only …

Population Demography Of Oldham’S Leaf Turtle (Cyclemys Oldhamii) In Protected And Disturbed Habitats In Thailand, Sengvilay Seateun, Nancy E. Karraker, Bryan L. Stuart, Anchalee Aowphol

Natural Resources Science Faculty Publications

Background. Freshwater turtle populations are vulnerable to a range of human activities because of particular life history attributes, and anthropogenic impacts can cause shifts in demographic traits, including survival, density and population structure. Asian freshwater turtles have undergone dramatic population declines in recent decades principally because of collection for food, pet, and traditional medicine markets. Despite this, few studies have been conducted on the population demography of these turtles, thereby limiting our understanding of population trends and the development of conservation actions. Oldham's leaf turtle (Cyclemys oldhamii) is one of the most commonly traded turtles in Asian …

Evidence For Activated Lck Protein Tyrosine Kinase As The Driver Of Proliferation In Acute Myeloid Leukemia Cell, Ctv-1, Li Li, Yixin Cui, Jinyan Shen, Hannah Dobson, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Acute myeloid leukemia (AML) is a heterogeneous group of fast growing cancers of myeloid progenitor cells, for which effective treatments are still lacking. Identification of signaling inhibitors that block their proliferation could reveal the proliferative mechanism of a given leukemia cell, and provide small molecule drugs for targeted therapy for AML. In this study, kinase inhibitors that block the majority of cancer signaling pathways are evaluated for their inhibition of two AML cell lines of the M5 subtypes, CTV-1 and THP-1. While THP-1 cells do not respond to any of these inhibitors, CTV-1 cells are potently inhibited by dasatinib, bosutinib, …

Planarity And Out-Of-Plane Vibrational Modes Of Tryptophan And Tyrosine In Biomolecular Modeling, Faramarz Joodaki, Lenore M. Martin, Michael L. Greenfield

Cell and Molecular Biology Faculty Publications

Tryptophan and tyrosine are aromatic amino acids that play significant roles in the folding processes of proteins at water-membrane interfaces because of their amphipathic structures. Employing appropriate heteroaromatic molecular structures are essential for obtaining accurate dynamics and predictive capabilities in molecular simulations of these amino acids. In this study, molecular dynamics simulations that applied the most recent version of the CHARMM36 force field were conducted on aqueous solutions of tryptophan and of tyrosine. Geometric analysis and dynamics quantified how aromatic rings deviated from planar structures and exhibited out-of-plane fluctuations. Radial distribution functions showed possible biological significance because extent of ring …

Immune Escape And Immune Camouflage May Reduce The Efficacy Of Rts,S Vaccine In Malawi, Sundos Khan, Matthew Parrillo, Andres H. Gutierrez, Frances E. Terry, Leonard Moise, William D. Martin, Anne S. De Groot

Cell and Molecular Biology Faculty Publications

The RTS,S/AS01 malaria vaccine will undergo a pilot vaccination study in sub-Saharan Africa beginning in 2019. RTS,S/AS01 Phase III trials reported an efficacy of 28.3% (children 5–17 months) and 18.3% (infants 6–12 weeks), with substantial variability across study sites. We postulated that the relatively low efficacy of the RTS,S vaccine and variability across sites may be due to lack of T-cell epitopes in the vaccine antigen, and due to the HLA distribution of the vaccinated population, and/or due to ‘immune camouflage’, an immune escape mechanism. To examine these hypotheses, we used immunoinformatics tools to compare T helper epitopes contained in …

Ponatinib Inhibits Proliferation And Induces Apoptosis Of Liver Cancer Cells, But Its Efficacy Is Compromised By Its Activation On Pdk1/Akt/Mtor Signaling, Chang Liu, Xiuli Mu, Xuan Wang, Chan Zhang, Lina Zhang, Baofeng Yu, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Ponatinib is a multi-target protein tyrosine kinase inhibitor, and its effects on hepatocellular carcinoma cells have not been previously explored. In the present study, we investigated its effects on hepatocellular carcinoma cell growth and the underlying mechanisms. Toward SK-Hep-1 and SNU-423 cells, ponatinib induces apoptosis by upregulation of cleaved caspase-3 and -7 and promotes cell cycle arrest in the G1 phase by inhibiting CDK4/6/CyclinD1 complex and phosphorylation of retinoblastoma protein. It inhibits the growth-stimulating mitogen-activated protein (MAP) kinase pathway, the phosphorylation of Src on both negative and positive regulation sites, and Jak2 and Stat3 phosphorylation. Surprisingly, it also activates the …

The Impact Of Co2 Evolved From Vc And Fec During Formation Of Graphite Anodes In Lithium-Ion Batteries, K. Uta Schwenke, Sophie Solchenbach, Julien Demeaux, Brett L. Lucht, Hubert A. Gasteiger

Chemistry Faculty Publications

Additives such as vinylene carbonate (VC) and fluoroethylene carbonate (FEC) are commonly added to lithium-ion battery electrolytes in order to form a solid electrolyte interphase (SEI) on the anode, suppressing continuous solvent reduction. In this work, we directly compare VC and FEC by analyzing the SEI with FTIR and XPS, and the evolved gases with on-line electrochemical mass spectrometry (OEMS) in different model systems. Since both additives evolve mainly CO2 during formation, the effect of CO2 as an additive is compared to the addition of VC and FEC. While Li2CO3 is as expected the main SEI compound found due to …

Dietary Diversity Was Positively Associated With Psychological Resilience Among Elders: A Population-Based Study, Zhaoxue Yin, Melanie Sereny Brasher, Virginia B. Kraus, Yuebin Lv, Xiaoming Shi, Yi Zeng

Sociology and Anthropology Faculty Publications

The association between dietary diversity (DD) and psychological resilience among older people is an underdeveloped area of research. This cross-sectional study explored the associations of DD with psychological resilience among 8571 community-based elderly individuals. The intake frequencies of food groups were collected, and dietary diversity was assessed based on the mean DD score. Psychological resilience was assessed using a simplified resilience score (SRS). Data were analyzed using multiple linear regression and logistic regression models. Poor DD was significantly associated with psychological resilience, with a β (95% CI) of −0.94 (−1.07, −0.81) for the SRS (p < 0.01) and an odds ratio (95% CI) of 1.83 (1.66, 2.01) for low SRS status. The interaction effects of age with DD were observed for the SRS (p < 0.001) and low SRS status (p < 0.001). Based on separate analyses by age group, the association of a low SRS with poor DD was more prominent in the younger elderly than the oldest old, with OR (95% CI) 2.32 (1.96, 2.74) and 1.61 (1.43, 1.82), respectively. Compared with younger participants with good DD, the risk of a low SRS was greater for younger participants with poor DD, the oldest old with good DD, and the oldest old with poor DD, with OR (95% CI) 2.39 (2.02, 2.81), 1.28 (1.09, 1.51), and 2.03 (1.72, 2.39), respectively. The greatest contribution to DD was from a high consumption of vegetables, fruits, and nuts. Our study suggested that poor DD was associated with a low psychological resilience among the Chinese elderly, especially the younger elderly. These findings suggest that augmentation of DD might promote psychological resilience.

Urbanization Affects Body Size And Parasitism But Not Thermal Preferences In Anolis Lizards, Christopher J. Thawley, Haley A. Moniz, Amanda J. Merritt, Andrew C. Battles, Sozos N. Michaelides, Jason J. Kolbe

Biological Sciences Faculty Publications

Urbanization is accelerating worldwide and creates novel habitat conditions including increases in environmental temperature and changes in presence and abundance of predators, prey and parasites. For species that use urban habitats, these changes can have strong impacts on phenotypes. Anolis lizards commonly exploit urban habitats and, as ectotherms, are likely to experience pressures from these novel environments. Previous research shows that anoles may adapt to some aspects of urban habitats, but we lack an understanding of the breadth of traits that may shift in response to urbanization and how widespread these changes may be. To assess effects of urban habitat …

A Comparison Of The Population Genetic Structure And Diversity Between A Common (Chrysemys P. Picta) And An Endangered (Clemmys Guttata) Freshwater Turtle, Scott W. Buchanan, Jason J. Kolbe, Johanna E. Wegener, Jessica R. Atutubo, Nancy E. Karraker

Biological Sciences Faculty Publications

The northeastern United States has experienced dramatic alteration to its landscape since the time of European settlement. This alteration has had major impacts on the distribution and abundance of wildlife populations, but the legacy of this landscape change remains largely unexplored for most species of freshwater turtles. We used microsatellite markers to characterize and compare the population genetic structure and diversity between an abundant generalist, the eastern painted turtle (Chrysemys p. picta), and the rare, more specialized, spotted turtle (Clemmys guttata) in Rhode Island, USA. We predicted that because spotted turtles have disproportionately experienced the detrimental effects of habitat loss …

Even Obligate Symbioses Show Signs Of Ecological Contingency: Impacts Of Symbiosis For An Invasive Stinkbug Are Mediated By Host Plant Context, Janelle Couret, Lynn Huynh-Griuffin, Ivan Antolic-Soban, Tarik Salam Acevedo-Gonzalez, Nicole M. Gerardo

Biological Sciences Faculty Publications

Many species interactions are dependent on environmental context, yet the benefits of obligate, mutualistic microbial symbioses to their hosts are typically assumed to be universal across environments. We directly tested this assumption, focusing on the symbiosis between the sap‐feeding insect Megacopta cribraria and its primary bacterial symbiont Candidatus Ishikawaella capsulata. We assessed host development time, survival, and body size in the presence and absence of the symbiont on two alternative host plants and in the insects' new invasive range. We found that association with the symbiont was critical for host survival to adulthood when reared on either host plant, with …

Graduate School Of Oceanography Annual Report For Fy2019, Uri Graduate School Of Oceanography

Graduate School of Oceanography Publications

Annual report for the Graduate School Oceanography of the University of Rhode Island for the 2019 fiscal year.

Includes research projects by faculty and students, biographies of new faculty, events on the Bay campus and program development and list of donors.

Scaffold-Based Lung Tumor Culture On Porous Plga Microparticle Substrates, Aneetta E. Kuriakose, Wenjing Hu, Kytai T. Nguyen, Jyothi Menon

Biomedical and Pharmaceutical Sciences Faculty Publications

Scaffold-based cancer cell culture techniques have been gaining prominence especially in the last two decades. These techniques can potentially overcome some of the limitations of current three-dimensional cell culture methods, such as uneven cell distribution, inadequate nutrient diffusion, and uncontrollable size of cell aggregates. Porous scaffolds can provide a convenient support for cell attachment, proliferation and migration, and also allows diffusion of oxygen, nutrients and waste. In this paper, a comparative study was done on porous poly (lactic-co-glycolic acid) (PLGA) microparticles prepared using three porogens—gelatin, sodium bicarbonate (SBC) or novel poly N-isopropylacrylamide [PNIPAAm] particles, as substrates for lung cancer cell …

Rapid Preparation Of Spherical Granules Via The Melt Centrifugal Atomization Technique, Yan Yang, Nan Zheng, Xiaoyue Wang, Ryan Ivone, Weiguang Shan, Jie Shen

Biomedical and Pharmaceutical Sciences Faculty Publications

Granules with superior fluidity and low moisture absorption are ideal for tableting and capsule filling. Melt granulation as a solvent-free technology has attracted increasing interest for the granulation of moisture-sensitive drugs. The objective of the present study was to develop a solvent-less and high throughput melt granulation method via the melt centrifugal atomization (MCA) technique. The granule formability of various drugs and excipients via MCA and their dissolution properties were studied. It was found that the yield, fluidity, and moisture resistance of the granules were affected by the drug and excipient types, operation temperature, and collector diameter. The drugs were …

Antagonism Of Quorum Sensing Phenotypes By Analogs Of The Marine Bacterial Secondary Metabolite 3-Methyl-N-(2′-Phenylethyl)-Butyramide, Susan M. Meschwitz, Margaret E. Teasdale, Ann Mozzer, Nicole Martin, Jiayuan Liu, Stephanie Forschner-Dancause, David C. Rowley

Biomedical and Pharmaceutical Sciences Faculty Publications

Quorum sensing (QS) antagonists have been proposed as novel therapeutic agents to combat bacterial infections. We previously reported that the secondary metabolite 3-methyl-N-(2′-phenylethyl)-butyramide, produced by a marine bacterium identified as Halobacillus salinus, inhibits QS controlled phenotypes in multiple Gram-negative reporter strains. Here we report that N-phenethyl hexanamide, a structurally-related compound produced by the marine bacterium Vibrio neptunius, similarly demonstrates QS inhibitory properties. To more fully explore structure–activity relationships within this new class of QS inhibitors, a panel of twenty analogs was synthesized and biologically evaluated. Several compounds were identified with increased attenuation of QS-regulated phenotypes, most notably N-(4-fluorophenyl)-3-phenylpropanamide against the …

Understanding Electrolyte Decomposition Of Graphite/Ncm811 Cells At Elevated Operating Voltage, Nina Laszczynski, Sophie Solchenbach, Hubert A. Gasteiger, Brett L. Lucht

Chemistry Faculty Publications

Cells containing LiNixCoyMn1-x-yO2 (NCM) materials with very high nickel content suffer from cathode material degradation upon cycling, especially when operating these cells at higher voltage. While the cathode particle degradation is a problem other issues contribute to electrochemical performance loss. Graphite/LiNi0.8Co0.1Mn0.1O2 cells have been cycled to different upper cutoff voltages and investigated with electrochemical impedance spectroscopy (EIS), gas analysis with online electrochemical mass spectroscopy (OEMS), nuclear magnetic resonance (NMR) spectroscopy, gas chromatography with MS (GCMS) and inductively coupled plasma MS (ICP-MS). Increased gas evolution was observed when the upper cutoff voltage was increased from 4.2 to 4.6 V. An increase …

Drug Sensitivity Screening And Targeted Pathway Analysis Reveal A Multi-Driver Proliferative Mechanism And Suggest A Strategy Of Combination Targeted Therapy For Colorectal Cancer Cells, Jinyan Shen, Li Li, Tao Yang, Niuliang Cheng, Gongqin Sun

Cell and Molecular Biology Faculty Publications

Treatment of colorectal cancer mostly relies on traditional therapeutic approaches, such as surgery and chemotherapy. Limited options of targeted therapy for colorectal cancer narrowly focus on blocking cancer-generic targets VEGFR and EGFR. Identifying the oncogenic drivers, understanding their contribution to proliferation, and finding inhibitors to block such drivers are the keys to developing targeted therapy for colorectal cancer. In this study, ten colorectal cancer cell lines were screened against a panel of protein kinase inhibitors blocking key oncogenic signaling pathways. The results show that four of the 10 cell lines did not respond to any kinase inhibitors significantly, the other …