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Tandem Conjugate Addition -- Cyclization Reactions Of L-Methyl Prolinate With α,β-Unsaturated Ketones Catalyzed By L-Proline, Sergio Sandoval

Open Access Dissertations

L-methyl prolinate and various α,β-unsaturated ketones were reacted by a tandem sequence of conjugate addition and subsequent autocatalytic intramolecular cyclization to yield 1,3-dicarbonyl-pyrrolizidine ring systems in modest yield. This methodology represents a tandem and green preparative reaction sequence where L-proline is self-generated as an organocatalyst subsequent to the first step and prior to the second step of the process. This sequence of reactions is a one-pot, tandem, and green preparative reaction for the synthesis of various pyrrolizidine derivatives which can be performed in a general and simple way under solventless and room temperature conditions. This methodology is modest yielding and …

Alzheimer's Disease (Ad) Like Pathology Following Developmental Lead Exposure In Primates And The Role Of Aging In Ad-Related Genes Regulation In Rodents And Primates, Jinfang Wu

Open Access Dissertations

Alzheimer's disease is a progressive, irreversible neurodegenerative disease results in memory loss and dementia. The search for the etiology of sporadic AD focuses on environmental links and the role of aging. In manuscript 1, we validated our previous rodent model in primates by the following findings. (1) Developmental Pb exposure caused elevated AD-related APP, BACE1 and Sp1 mRNA expression as well as APP protein and amyloidogenic Aβ40 and Aβ42. (2) The molecular changes were accompanied by AD-like pathological changes in exposed monkey brains. (3) Pb exposure increased 8-oxo-2'-deoxyguanine in the monkey brain. (4) DNA methyltransferase activity was suppressed in exposed …

Study On The Nonenzymatic Glycation Of Nucleosides/Nucleotides And Proteins With Sugars: An In Vitro Investigation Of Advanced Glycation Endproducts (Ages) Formation, Yuyuan Li

Open Access Dissertations

Advanced glycation endproducts (AGEs) are a complex and heterogeneous group 1 of compounds formed by the nonenzymatic reactions of the carbonyl groups of reducing sugars with the free amino groups of biomolecules. Nonenzymatic reactions can occur in vitro and in vivo and affect the structure, function and conformation of many biomolecules, and AGEs has been suggested to associate with the pathogenesis of various disease such as diabetes and Alzheimer disease. The increased number of reports on the nonenzymatic glycation of proteins and nucleic acids has generated great interest in the methods for characterization of AGEs. The lack of universally applicable …

Microstructure Studies In Synthetic And Bio-Colloids, Imaging And Applications, Ashish Kumar Jha

Open Access Dissertations

The mean size of CTAB/HDBS mixed surfactant vesicles confined into the three-dimensional voids generated by the random close packing (RCP) of polystyrene beads, shows a systematic decrease as the characteristic confinement length scale is reduced. The RCP of polystyrene beads of radius Rb = 1.5μm, 0.25μm and 0.1μm, creates voids of characteristic dimensions R ∼ 0.22 Rb = 3300Ã…, 550Ã… and 220Ã… respectively. These void length scales are comparable to or less than the radii of vesicles formed in the system under conditions of no confinement. A simple thermodynamic model was developed accounting for the balance between increased enthalpy when …

Fabrication, Characterization And Application Of The Novel Bionanomaterials, Lan Yao

Open Access Dissertations

This dissertation focuses on developing methods to synthesize novel bionanomaterials, studying their properties and exploring their potential applications. Fabricating semiconducting nanowires is a significant challenge in making nanoscale electronic devices. We propose to use self-assembling biomolecules as templates for the fabrication of nanowires. Here we demonstrate that quantum dots (QDs) could be conjugated to actin filaments, forming long semiconductor filaments. We constructed an optical system and developed the interactive software for fluorescence anisotropy imaging of single QDs and QD-nanowires. The fluorescence anisotropy studies revealed that QD transition dipoles are likely circular (elliptical) dipoles. The fluorescence anisotropy of a single QD …

A Concurrent Validation Of The Violence Sensitivity Scale, Jonathan Corey

Open Access Dissertations

A correlational concurrent validity study of the Violence Sensitivity Scale (VSS) was conducted in which respondents (N=169) also completed the Nonviolence Test (NVT), the Attitudes Toward Violence Scale (ATVS) and the Marlowe-Crowne Social Desirability Scale (MCSD). The VSS was significantly positively correlated with the NVT and MCSD, and significantly negatively correlated with a transformed ATVS distribution. Principal Factors Analysis limited to four, factors assigned 23 of 38 VSS behaviors to the same factors as in a previous study. Multiple regression tests found the NVT and MCSD to be significant predictors of VSS and VSS subscale scores. Demographic variables were examined …

Chemistry Of Antibiotics From Atlantic Actinomycete And Bacillus Bacteria, Aaron Martin Socha

Open Access Dissertations

The emergence of drug-resistant pathogens requires the use of innovative therapeutic agents. Hospital-acquired (nosocomial) infections represent major health and economic burdens for patients and health care professionals. In the United States over 1 million nosocomial infections are caused by drug-resistant bacteria anually, with associated expenditures ranging from $4 to $30 billion. Methicillin-resistant Staphylococcus aureus (MRSA) commonly shows reduced susceptibility to β-lactam, peptide, macrolide, aminoglycoside, tetracycline, and lincosamide classes of antibiotics. The emergence of vancomycin-resistant enterococci (VRE) is even more alarming since glycopeptide antibiotics were once considered the drugs of last resort. Moreover, community-acquired MRSA infections are spreading out of the …

Applications Of Linear And Cyclic Peptides As Enzyme Inhibitors And Molecular Transporters, Guofeng Ye

Open Access Dissertations

The diversity of amino acids in peptides allows the design of different linear or cyclized compounds. Peptides can be designed to act as inhibitors, probes, or molecular transporters of other molecules. The objective of this dissertation research was to exploit the diversity of peptide structures and focused on developing linear and cyclic peptides as c-Src inhibitors and using the information derived from c-Src crystal structure to design novel peptide-based molecular transporters. A series of peptide analogues of Ac-CIYKYY were designed and synthesized to improve the inhibitory potency against active Src Linear peptide Ac-CIYKF(4-NO 2)Y (IC50 = 0.53 μM) and a …

Design And Evaluation Of Nucleoside Derivatives For Targeted Drug Delivery And Therapeutic Applications, Hitesh Kumar Agarwal

Open Access Dissertations

2',3'-Dideoxynucleoside analogs are commonly used as anti-HIV, anti-HBV, and anti-cancer drugs. Despite of their potent activities, there are some major limitations in using 2',3'-dideoxynucleosides as therapeutic agents. The nucleosides have usually poor cellular uptake because of their hydrophilic nature. Some of the nucleoside analogs, such as anti-HIV agents, become ineffective after multiple administrations because of the development of the drug resistance, and therefore they must be administered in combination therapy. It is hard to deliver the nucleoside analogs to a particular tissue for site specific targeting. Furthermore, nucleoside analogs undergo three intracellular phosphorylation steps to become active. The first phosphorylation …

Acoustic Conditioning To Reduce The Impact Of Escapement In Atlantic Salmon (Salmo Salar) Net Pen Aquaculture, Jennifer Andrew

Open Access Master's Theses

Net pen culture is the most common method used to raise salmon, however these farms are typically located in areas through which wild salmon inhabit or migrate and escapes may influence wild stocks. Commercial salmon farms can potentially reduce both economic losses to the producer and impacts on wild stocks by recapturing escapees. Acoustic signaling has been used to concentrate fish species for feeding or harvest and to deter fish from specific areas. The present study investigated the approach of conditioning salmon to associate a tone with feeding to entice return to a specific location allowing recapture. The ability of …

Using Acidiphilium Acidiphilium To Bioleach Metals From Printed Circuit Boards, Diana Goglia Arora

Open Access Master's Theses

As many as 500 million computers became obsolete between the years of 2000 and 2007 (U.S. EPA, 2007), due in large part to the increasing rate of technological advances. Printed circuit boards (PCB) are the essential components of most electronic waste. Metals that are contained within PCB are potentially hazardous to the environment. These metals include copper, aluminum, chromium, mercury, zinc, lead, and nickel (Li et. al., 2004). If not contained properly, metals can leach into soils and possibly damage the ecosystem. This problem of growing electronic waste not only exists in the USA, but it also extends overseas in …

In Vitro Effects Of Emollient Substances On Release Of Ketorolac Tromethamine, Burcak Alioglu

Open Access Master's Theses

Ketorolac tromethamine is a non-steroidal drug that has potent analgesic and moderate anti-inflammatory activity. A single dose of ketorolac tromethamine is clinically more effective than that of many drugs in the management of moderate to severe postoperative pain. Transdermal delivery of ketorolac tromethamine seems as a potential method of administration that would be non-invasive and eliminate repeated dosing regimens. Although a transdermal dosage form of ketorolac has not arrived in the market, permeation enhancement of ketorolac tromethamine transdermally and topically has been the subject of several pharmaceutical research studies.

The objective of this study was to evaluate the possible release …

Shanthi Sena: Unarmed Peacekeeping, Dennis August Almeida

Open Access Master's Theses

Where questions of violence and nonviolence cannot be raised inside a polity, it becomes obligatory that social scientists undertake them from the outside (Paige 2002:92). And if we are to "transform our society from a psychology of killing and threats to kill to one that is life-enhancing and spiritually fulfilling," (Ariyaratne 1999:73) then social science research on nonviolence and unarmed peacekeeping is an imperative. With this in mind I investigated the Shanthi Sena (Peace Brigade), an independent unit of the Sarvodaya Sharamadana Movement in Sri Lanka, through a six week in-depth case study; a full description, understanding and appreciation of …

A Monomeric Membrane Peptide That Lives In Three Worlds: In Solution, Attached To, And Inserted Across Lipid Bilayers, Yana K. Reshetnyak, Michael Segala, Oleg A. Andreev, Donald M. Engelman

Physics Faculty Publications

The membrane peptide pH (low) insertion peptide (pHLIP) lives in three worlds, being soluble in aqueous solution at pH 7.4, binding to the surface of lipid bilayers, and inserting as a transbilayer helix at low pH. With low pH driving the process, pHLIP can translocate cargo molecules attached to its C-terminus via a disulfide and release them in the cytoplasm of a cell. Here we examine a key aspect of the mechanism, showing that pHLIP is monomeric in each of its three major states: soluble in water near neutral pH (state I), bound to the surface of a membrane near …

Serials Unit Annual Report 2006-2007, Andree J. Rathemacher

Serials Unit Reports

Annual report of the Serials Unit, University of Rhode Island Library. Covers personnel, work flow, subscriptions, and statistics on expenditures by a number of variables.

Serials Solutions Subject Search Report 2006-2007

Technical Services Reports and Statistics

Statistics on the total number of Serials Solutions subject searches at the University of Rhode Island Libraries by month for 2006-2007. Shows how many times the A-Z full text electronic journal list (at http://zu4tq4pb5v.search.serialssolutions.com/) was browsed by specific subjects.

Reference Database Usage Statistics 2006-2007, Andree J. Rathemacher

Technical Services Reports and Statistics

Statistics on the use of online reference databases at the University of Rhode Island during the period from July 2006 through June 2007. Includes COUNTER-compliant data on sessions, searches, turnaways, full text articles downloaded, cost per search, and other metrics.

Mechanism And Uses Of A Membrane Peptide That Targets Tumors And Other Acidic Tissues In Vivo, Oleg A. Andreev, Alison D. Dupuy, Michael Segala, Srikanth Sandugu, David A. Serra, Clinton O. Chichester, Donald M. Engelman, Yana K. Reshetnyak

Physics Faculty Publications

The pH-selective insertion and folding of a membrane peptide, pHLIP [pH (low) insertion peptide], can be used to target acidic tissue in vivo, including acidic foci in tumors, kidneys, and inflammatory sites. In a mouse breast adenocarcinoma model, fluorescently labeled pHLIP finds solid acidic tumors with high accuracy and accumulates in them even at a very early stage of tumor development. The fluorescence signal is stable for >4 days and is approximately five times higher in tumors than in healthy counterpart tissue. In a rat antigen-induced arthritis model, pHLIP preferentially accumulates in inflammatory foci. pHLIP also maps the renal …

Darfur: Genocide In The 21st Century, Victoria Goff

Senior Honors Projects

The conflict in Darfur has been described as the “world’s worst humanitarian crisis” by the United Nations, and the United States has condemned the war as genocide. But four years later, the death toll of 200,000 continues to rise. At least another 2.5 million have been displaced, and neighboring countries have declared a state of emergency. Unless something is done to stop the violence, the chaos will continue to spread. Frustrated with lack of representation in the government, rebel groups from Darfur – the western region of Africa’s largest country, Sudan – revolted against its national government in 2003. The …

A Comparison Of Pyronin Y-Methyl Green Stain And Methylene Blue Stain For Somatic Cell Count In Sheep Milk, Emily Mirek, Stacey O’Donnell

Senior Honors Projects

Somatic cell count is a key method used to evaluate the quality of milk in today’s growing dairy sheep industry. Somatic cells are body cells, primarily the white blood cells, found in a milk sample. If an infection such as mastitis is present, the number of somatic cells in the milk increases (Gonzalo, et al. 1992). Producers routinely perform somatic cell counts on cow and goat milk. For dairy cattle in the United States, the somatic cell count can not exceed 750,000 cells per milliliter, for commercial milk. For dairy goats the accepted limit is 1,000,000 cells per mL of …

Mechanics Of Suction Generation During Feeding In Little Skates, Danielle Duquette

Senior Honors Projects

Feeding mechanisms of aquatic vertebrates has been extensively studied in the past, while that of elasmobranchs remains limited. Skates and rays are believed to have evolved from a shark ancestor, thus they represent the most derived group. All skates are dorsoventrally compressed, have a unique jaw suspension type and head skeleton and live in benthic environments. It is unknown whether these derived features of skates have altered the ancestral shark feeding mechanism. Comparing feeding mechanisms in skates and sharks may shed light on morphological transformations that have arisen after the evolutionary split of these two groups. In this study prey …

Invasive Alien Species In An Era Of Globalization, Laura A. Meyerson, Harold A. Mooney

Natural Resources Science Faculty Publications

Globalization facilitates the spread of invasive alien species (IAS) as international commerce develops new trade routes, markets, and products. New technologies increase the pace at which humans and commodities can move around the world. Recent research on IAS at the global scale has examined commerce and travel in order to inform predictions, risk analyses, and policy. Due to limited data, regional-scale studies have primarily focused on invasion patterns rather than impacts. Local-scale experimental research can identify mechanisms and impacts of biological invasions, but the results may not be applicable at larger spatial scales. However, the number of information networks devoted …

Nietzsche’S Doctrine Of Eternal Return, David R. Gadon

Senior Honors Projects

In Thus Spoke Zarathustra, Friedrich Nietzsche challenges the reader to imagine that every moment of his or her life, every joy and every sorrow, every smile and every tear, will be repeated in exactly the same way from beginning to end. He asks us to envision these things recurring, not one more time, but for all infinity into the past and future alike. Amongst the many revolutionary and profound concepts put forward by the witty and vitriolic Nietzsche, there is none as inscrutable and seemingly inapproachable as this doctrine of eternal return of the same. In just three of his …

Students Sharing Feelings Of Grief: An Elementary Curriculum On Loss, Kristen Gloumakoff

Senior Honors Projects

“It is only natural that we and our children find many things that are hard to talk about. But anything human is mentionable and anything mentionable is manageable.The mentioning can be difficult, and the managing too, but both can be done if we’re surrounded by love and trust.” ~Fred Rogers This quote could be talking about many different things that parents find difficult to talk to their children about, such as smoking, drinking, drugs, divorce, and sex. I was introduced to this quote as a one about parents talking to their children about death. Death is a part of life, …

Beliefs Of Violence-Sensitive And Violence-Tolerant People, Amethyst Brell

Senior Honors Projects

Every person views the world through a different lens, and one can never fully understand the motivations and opinions of someone else. However, experiments and statistics can shed some light onto people’s thought patterns and behaviors. When horrific acts of violence occur, many people strive to understand why they happen. Through my study I hope to help solve some of the mysteries behind the different ways people interpret violence. I want to see why some people tend to be more sensitive to violence and why some are more tolerant of violence. I am doing this by building on previous research …

A Pre-Apocalyptic Masterpiece, Brad Orleck

Senior Honors Projects

This article or section or project is not written in the formal tone expected of an honors project. Please improve it or discuss changes on the talk page. See URI's guide to writing better articles for suggestions. For years, various groups have predicted the end of the world, usually a spiritual or nuclear armageddon. Religious groups have predicted the second coming, Hollywood directors have predicted devastating wars with artificial intelligence, and conspiracy theorists predicted Y2K, among others. But every year, the world keeps not ending (widely regarded by scholars as a good thing). And while the world may not end …

Toward Salvation: Italo Calvino’S Wakeful Phenomenology, May C. Peckham

Senior Honors Projects

“Lightness.” The word remains inescapable when attending to the mysterious work of Italo Calvino. It appears elusively in the texts of his novels and acts as a catalyst to many of his critical endeavors. Calvino addresses most explicitly this concept of lightness in his collection of lectures entitled Six Memos for the Next Millennium. Although these lectures were never delivered, they exist as a testament to the idea that “the boundless universe of literature” contains “new avenues to be explored, both very recent and very ancient, styles and forms that can change our image of the world” (Six Memos 7-8). …

The Power Of Ridicule: An Analysis Of Satire, Megan Leboeuf

Senior Honors Projects

Satire is an art form that has existed throughout recorded history. Examples of satirical work exist from long before the genre had even been defined, and this powerful tool for social critique is alive and well today, perhaps even more prevalent than ever. The use of absurdity and often humor to demonstrate the problems with a particular human behavior, vice, or social issue makes satire engaging and persuasive in a way that a direct statement of the facts is not. These qualities make satire the perfect tool for advocating social and political change in times of unrest. In recent years, …

La Mondialisation Qui Menace L’Identité Française Et Ses Relations Avec Le Commerce, Kelly Mcbrien

Senior Honors Projects

Globalization is an integral part of our society today: economically, socially and politically. Some may see Globalization as the world coming together through the ease and speed of capital, goods, services, ideas, information, and technology across our “shrinking” borders. Others may hold a more negative view of Globalization, and may see it as simply growing conflicts between nations and cultures. One of the central problems of globalization is the fear of homogenization or Americanization. Many cultures see globalization as cultural uniformity. As Benedict Anderson has said, “one man’s imagined community is another mans political prison.” This quote can help to …

Importance Of Early Successional Forest For Wildlife In Southern New England, Amy Wynia

Senior Honors Projects

Many bird species that require early successional forest are declining in the Northeast U.S. because such habitat is relatively rare and when they inhabit the more common mature forests or suburban areas they are less successful. Early successional forest is maintained by regular disturbance (wind, fire, clear-cutting, and flooding) which has been happening less frequently during the past 50 years. Bird species that have declined during this time and which inhabit early successional forest include ruffed grouse (Bonasa umbellus), blue jay (Cyanocitta cristata), chestnut-sided warbler (Dendroica pensylvanica), gray catbird (Dumetella carolinensis), field sparrow (Spizella pusilla), golden-winged warbler (Vermivora chrysoptera), eastern …