Digital Commons Network^™

The Biology And Population Ecology Of Deep-Sea Red Crabs, Chaceon Spp. In The North Atlantic Ocean, Imam Syuhada

Open Access Dissertations

Red crab (Chaceon spp.) fishery resources exist on both sides on the North Atlantic, and the fisheries that harvest these resources seek to maintain their sustainability. To be able to conduct fishery assessments with less uncertainty, resource managers need a better understanding of the life history characteristics of the species, more recent information on the abundance and distribution of the resource, and finally reliable estimates of the levels of exploitation and the effects of harvesting of the resources. This study contributes to the body of knowledge of the resource in the western Atlantic resource and red crab fishery …

Liposomes For The Controlled Delivery Of Multiple Drugs, Swapnil A. Malekar

Open Access Dissertations

Various pathological conditions, such as cancer, osteoporosis, AIDS, tuberculosis, and other pathologies, require the concurrent use of multiple drugs, known as combination therapy or polytherapy, for effective treatment. The concurrent delivery of multiple drugs in a single dosage form, such as a tablet, capsule or parenteral, has been demonstrated in a number of products, but current technologies for multiple drug delivery remain limited for broad application. Physicochemical incompatibility, limited aqueous and lipid solubility, and chemical instability of the individual drugs, as well as detrimental drug-drug and drug-excipient interactions within the multiple-component dosage form, may all compromise the development of stable, …

A Genomic And Electrophysiological Study Of Photoreception In The Eyeless Cnidarian Hydra Vulgaris, Stephanie L. Guertin

Open Access Dissertations

Hydra are tubular coelenterates with two germ layers, the endoderm and ectoderm. A ring of five to eight tentacles surround an oral pore, and the animal attaches to the substrate via adhesion of the peduncle at the opposite end. They possess a complete ectodermal nerve net, with nerve fibers running within the ectoderm throughout the body and along the length of the tentacles (Hufnagel 1976.) Hydra has two nerve rings. One nerve ring surrounds the mouth and is thought to coordinate mouth opening. A second ring, located at the base of the tentacles, coordinates movements of the body, tentacles, mouth, …

Risk, Promotive, And Protective Influences On Adolescent Alcohol Use Milestones, Scott D. Martin

Open Access Dissertations

Research focused on the initiation and development of alcohol use among adolescents can inform professionals, families, and preventative strategies. Much of the research on adolescent substance use among this population focuses on risk. This study investigated a model of the initiation of adolescent alcohol use milestones including first full drink, first time engaging in heavy drinking, and first time being drunk, extending the risk perspective by emphasizing a model of resilience. This was done by simultaneously including risk, promotive, and protective influences in a single model. It was hypothesized that parental monitoring and reasons for abstaining and limiting drinking would …

Argument, Rhetoric, And Transcendence: “The Adherence Of Minds” Within The Discourse Of Spirituality, Gavin Forrest Hurley

Open Access Dissertations

My study, “Argument, Rhetoric, and Transcendence: The ‘Adherence of Minds’ within a Discourse of Spirituality,” addresses the questions: What persuades Americans to adhere to contemporary discourses of spirituality? What persuades audiences to adhere to the experiential truths found with this discourse? Furthermore, how exactly do contemporary writers of spirituality adhere audiences to reasonable understanding and pursuit of a union with a higher power? Using Chaim Perelman and Lucie Olbrechts-Tyteca’s The New Rhetoric as a framework, I analyze how the persuasive machinery of current Catholic and Anglican spirituality texts guides readers to experience and understand a subjective union with an ineffable …

Phytochemical And Biological Investigation Of Gallotannins From Red Maple (Acer Rubrum) Species, Hang Ma

Open Access Dissertations

This study investigated the phytochemical constituents, primarily gallotannins, present in a proprietary extract, namely MaplifaTM, from leaves of the red maple (Acer rubrum L.) species as well as their biological activities and mechanisms of action. Although the red maple species has been traditionally used as folk medicine by Native American Indians for numerous health benefits, the bioactive chemical constituents of the leaves of the red maple still remain unknown. This study carried out the identification of phytochemicals targeting gallotannins, a class of polyphenols, from red maple leaves by using various chromatographic separation techniques and spectroscopic approaches. Furthermore, compounds from the …

Evolutionary Potential Of Marine Phytoplankton Under Ocean Acidification, Sinéad Collins, Björn Rost, Tatiana A. Rynearson

Graduate School of Oceanography Faculty Publications

Marine phytoplankton have many obvious characters, such as rapid cell division rates and large population sizes, that give them the capacity to evolve in response to global change on timescales of weeks, months or decades. However, few studies directly investigate if this adaptive potential is likely to be realized. Because of this, evidence of to whether and how marine phytoplankton may evolve in response to global change is sparse. Here, we review studies that help predict evolutionary responses to global change in marine phytoplankton. We find limited support from experimental evolution that some taxa of marine phytoplankton may adapt to …

Mercury Bioaccumulation In Cartilaginous Fishes From Southern New England Coastal Waters: Contamination From A Trophic Ecology And Human Health Perspective, David L. Taylor, Nicholas J. Kutil, Anna J. Malek, Jeremy S. Collie

Graduate School of Oceanography Faculty Publications

This study examined total mercury (Hg) concentrations in cartilaginous fishes from Southern New England coastal waters, including smooth dogfish (Mustelus canis), spiny dogfish (Squalus acanthias), little skate (Leucoraja erinacea), and winter skate (Leucoraja ocellata). Total Hg in dogfish and skates were positively related to their respective body size and age, indicating Hg bioaccumulation in muscle tissue. There were also significant inter-species differences in Hg levels (mean ± 1 SD, mg Hg/kg dry weight, ppm): smooth dogfish (3.3 ± 2.1 ppm; n = 54) > spiny dogfish (1.1 ± 0.7 ppm; n = 124) …

Nutritional And Immunological Evaluation Of Soy-Based Diets For Summer Flounder (Paralichthys Dentatus) Aquaculture, Daniel Ward

Open Access Dissertations

Summer flounder aquaculture has progressed significantly in the last twenty years, and diets have been optimized to provide optimal nutrition, ensuring a high quality final product. Recently, the focus of research has evolved toward finding a replacement for fish meal in diets for many species, as the demand for fish meal worldwide has caused the primary protein product to become increasingly economically volatile. Therefore, a series of experiments were designed to systematically evaluate feeds for summer flounder (Paralichthys dentatus) including soy-based products as the primary protein source. The first feed trial involved the comparison of three diets; a …

Prediction Of Structure Borne Noise Radiation And Propagation From Offshore Impact Pile Driving, Huikwan Kim

Open Access Dissertations

This study investigates prediction of structure borne noise radiation and propagation from offshore impact pile driving in shallow water environment. Noise generated by offshore impact pile driving radiates into and propagates through the air, water, and sediment medium. Predicting noise levels around the pile structure at sea is required to estimate the effects of the noise and vibration on marine life. This study used one of the commercial FE (Finite Element) code Abaqus 6.11 to calculate harmonic and transient response of the offshore wind turbine support structure and associated acoustic pressure amplitudes due to hydraulic hammer impact on top. First …

Phytochemical Investigation Of The Traditional Botanical Medicine Iresine Celosia, Caroline Grace Killian

Open Access Dissertations

Iresine celosia L. is a traditional medicine used by the indigenous Mayan people for a variety of ailments. It is also the sole active ingredient in the Odyliresin™ formulation currently marketed to humans worldwide as an antioxidant and for the promotion of prostate health. However, Odyliresin™ has not been characterized phytochemically, or evaluated biologically for its intended use. This work represents the first comprehensive phytochemical investigation of the Odyliresin™ formulation botanical extract. To better understand its constitutive phytochemistry, a tailored isolation scheme was developed, using various chromatography resins (silica gel, LH-20), separation and purification techniques. In all, eleven compounds were …

Situated Intercultural Communication: Domestic And International Student Interaction, Emily Spitzman

Open Access Dissertations

As higher education continues to internationalize, more institutions are making it an educational priority to increase intercultural competence among all students. Despite this goal, institutions regularly place students in intercultural programs without facilitating training and reflection on intercultural learning, with the expectation that students will learn from contact alone. There is a need for investigation into situated intercultural communication, for the limited studies that do examine interactions between international and domestic students do not look at the interactions themselves, do not situate the interactions in a specific context, and often examine only the students‘ international/domestic statuses or countries of origin …

An Investigation Of The Relationship Between School Leadership, Teacher Job Satisfaction, And Student Achievement, Beth-Ann Tek

Open Access Dissertations

This correlational cross-sectional study identifies and tests research-based constructs of school leadership and teacher job satisfaction on the 2012 Tell MASS survey using exploratory factor analyses, confirmatory factor analyses, and reliability analyses. Hierarchical linear modeling is used to examine the relationship between the survey’s school leadership and teacher job satisfaction scales. Multiple regression analyses are used to investigate the hypothesis that school leadership and student achievement on standardized tests in English Language Arts and Mathematics are also related, though this relationship is mediated by teacher job satisfaction.

Analyses revealed four major findings. First, EFA, CFA, and reliability analyses determined that …

Opting-In Online: Participants’ Perceptions Of Knowledge Construction In Public Forum Communities, Jennifer C. Lee

Open Access Dissertations

This project examines knowledge making and sharing amid special interest forums, specifically, online forums for herp (reptile and amphibian) hobbyists. In doing so, it addresses a gap in rhetoricians’ understanding of publicness: its effect on writing and people’s perceptions thereof. Few studies measure public writing unless its telos is change or activism. Additionally, despite increased rhetorical scholarship focusing on new media, much current research on forums situates them in classroom-based learning and collaboration, neglecting online forums that are open to public access. This project is a naturalistic inquiry using qualitative methods to explore collaborative public writing that occurs in discourse …

Firefighters’ Multimodal Literacy Practices, Timothy R. Amidon

Open Access Dissertations

This study explores the ways that firefighters’ utilize multimodal literacy practices to construct and communicate knowledge in environments where risk is present. Specifically, this dissertation sketches the interrelationships between alphabetic, aural, cognitive, gestural, kinesthetic, oral, tactile, spatial, and visual genres which evolve and circulate within embodied, analog, and digital media on firegrounds. A heuristic research methodology was constructed by borrowing and adapting components from extant multimodal and digital ethnography, community-based research, and writing activity and genre research. Using a range of emergent digital research methods, data was captured during six observational periods wherein 75 participants engaged in live-fire training activities; …

Intrapartum Nurses’ Beliefs About Childbirth: A Descriptive Qualitative Study In Nurses That Provide Care To Women In Labor And Birth, Sylvia P. Ross

Open Access Dissertations

In the United States, a large gap exists between what is known as best evidence in maternal-newborn health and the routine practices at the frontline of maternity care. Intrapartum nurses are uniquely positioned to promote practices that are evidencebased and support the normal physiologic process of birth. This qualitative study explored intrapartum nurses’ beliefs about childbirth and the influence of their beliefs on their clinical practice, particularly practices that promoted normal physiologic birth. Semi-structured qualitative interviews were conducted with 10 intrapartum registered nurses using Rubin and Rubin’s (2012) responsive interview model. All participants practiced on a labor and delivery unit …

The Anti-Epilepsy Potential And Antagonism Of The N-Methyl-D-Aspartate Receptor By Diaminodiphenyls, Kyle Robert Scully

Open Access Dissertations

Epilepsy is a disorder characterized by the occurrence of seizures, which are periods of abnormally excessive synchronous neuronal activity in the brain. Affecting over 70 million people worldwide, many of whom do not respond to pharmacotherapy, there is a need for novel anticonvulsant compound discovery. Diaminodiphenyl compounds, a class of compounds shown to present anticonvulsive effects in vivo have been purported to exert their effects on the N-methyl-D-aspartate receptor (NMDAr); an excitatory, ionotropic receptor that is a key player in the functions of the glutamatergic system. The glutamatergic system is vital in the promotion of synaptic plasticity and has …

Values And Services Of A Protected Riverine Estuary In East Africa: The Wami River And Saadani National Park, Catherine Grace Mcnally

Open Access Dissertations

The dialogue pertaining to the management of riverine and coastal ecosystems has evolved over the past decade to consider ecosystem goods and services due to their ability to link ecosystem structure and function to human well-being. Ecosystem services are “a wide range of conditions and processes through which natural ecosystems, and the species that are part of them, help sustain and fulfill human life” (Daily et al. 1997 p.2). Ecosystem goods emerge from ecosystem services and are defined as “organisms and their parts and products that grow in the wild and … are used directly for human benefits” (Daily et …

Fate And Removal Of Contaminants In Urban Environment, Varun K. Kasaraneni

Open Access Dissertations

Stormwater runoff from impermeable surfaces, such as roads, roof tops, and parking lots, is a cause of non-point source pollution problem. Sources such as fossil fuel combustion, automobiles, and other human and animal activities introduce high concentration of organic, inorganic and pathogenic contaminants in to environment. Stormwater runoff carrying these contaminants when untreated has the potential to impair water quality in the receiving water bodies such as surface and ground waters. The existing treatment systems are not reliable and needed improvements in order to meet water quality standards. Determining the levels and sources of contamination in an environment can help …

Transcriptional Regulation Of Aldo-Keto Reductase 1d1, Leila Valanejad

Open Access Dissertations

Bile acids are physiological detergents and surfactants with recently identified roles as signaling hormones. Maintenance of physiologically normal bile acids levels is fundamentally important as a deregulation within the bile acid synthesis pathway has the potential to result in an unbalanced bile acid pool. This may result in any number of pathological down stream effects and can exacerbate various diseases and disorders. Chenodeoxycholic acid (CDCA) and cholic acid (CA) are two major primary bile acids in humans with CDCA being more hydrophobic and toxic than CA. Aldo-keto reductase 1D1 (AKR1D1) and 12-alpha-hydroxylase (CYP8B1) are the two key enzymes responsible for …

The Bovine Spermatozoal Transcriptome And Sire Fertility, Christopher James Card

Open Access Dissertations

The goal of this dissertation research was to characterize the spermatozoal transcript profile of bovine cryopreserved spermatozoa. The main goal was to sequence the complete transcript profile from a population of sires across a wide fertility range utilizing RNA-Sequencing (RNA-Seq). Sequencing was then conducted to compare the spermatozoal transcript profiles of higher and lower fertility sires in an effort to determine the presence or absence of spermatozoal transcripts that may aid in improving fertility testing in sires. In addition to RNA-Seq analysis, other semen parameters such as morphology, DNA fragmentation, and RNA amount were analyzed for relationships with fertility.

The …

A Particle Swarm Optimization For The Vehicle Routing Problem, Choosak Pornsing

Open Access Dissertations

This dissertation is a study on the use of swarm methods for optimization, and is divided into three main parts. In the first part, two novel swarm metaheuristic algorithms -- named Survival Sub-swarms Adaptive Particle Swarm Optimization (SSS-APSO) and Survival Sub-swarms Adaptive Particle Swarm Optimization with velocity-line bouncing (SSS-APSO-vb) -- are developed. These new algorithms present self-adaptive inertia weight and time-varying adaptive swarm topology techniques. The objective of these new approaches is to avoid premature convergence by executing the exploration and exploitation stages simultaneously. Although proposed PSOs are fundamentally based on commonly modeled behaviors of swarming creatures, the novelty is …

Molecular Interaction In Carboxylesterases-Based Catalysis: Ontogenic Expression And Induction Through A Novel Element, Yi-Tzai Chen

Open Access Dissertations

Carboxylesterases (CES) catalyze both hydrolytic and synthetic reactions and play important roles in drug metabolism and lipid mobilization. Many factors such as age and dietary supplements have been shown to regulate the CES expression. The expression of CES1 shows the developmental regulation and is highly induced by antioxidants. The goals of this project were to determine whether CES2, like CES1, is developmentally regulated and to investigate how antioxidants induced the expression of CES1 at the molecular level.

The ontogenic studies showed the mRNA levels of CES2 exhibited a postnatal surge (1-31 days versus 35-70 days) in both liver and duodenum. …

The Green Eating Project: Web-Based Intervention To Promote Environmentally Conscious Eating Behaviors, Jessica T. Nash

Open Access Dissertations

The food system has been cited as unsustainable due to the reliance on natural resources and contribution to global pollution. Technological advances will play a major role in mitigating these negative consequences but consumers will also play a role through food choices. Green Eating (GE) is the concept of practicing more environmentally conscious eating behaviors and is currently defined as: eating locally grown foods, limited amounts of processed/fast foods, eating meatless meals at least one day per week, choosing organic foods as much as possible, and only taking what you plan on eating. Little research exists investigating college student perspectives’ …

Maternal Anxiety During And After Pregnancy: Effects On Child Internalizing Behaviour, Sue K. Adams, Jennifer F. Daly, Kathleen N. Morgan

Human Development and Family Science Faculty Publications

Background: Anxiety during pregnancy can have significant effects on both a developing fetus and long-term developmental outcomes. In animal and human models, prenatal anxiety has been associated with characteristics of inhibited temperament in infants, which may be a precursor to later anxiety disorders. This pilot study examined if maternal report of anxiety during pregnancy differentially explained preschool-aged children’s internalizing behaviour over and above a mother’s trait anxiety. In addition, using multi-reporter methodology, the study examined if maternal anxiety during pregnancy predicted multiple reporters’ ratings of child internalizing behaviour.

Method: Participants in this pilot study included preschool-aged children, their …

Comparison Of Phenotypic Value Changes In Pure Lines Of Bombyx Mori (Lepidoptera: Bombycidae) During Consecutive Generations Following Initial Selection On Cocoon Weight, Alireza Seidavi, Marian R. Goldsmith

Biological Sciences Faculty Publications

The experiments reported here were conducted to investigate the effect of selection on three quantitative traits, namely cocoon weight, cocoon shell weight, and cocoon shell percentage, during four generations by rearing six pure breeds of domesticated silkworm, Bombyx mori L. (Lepidoptera: Bombycidae) of Chinese and Japanese origin compared with random unselected groups as controls. All stages of rearing and data recording were performed over four rearing periods, with generations 1–3 during successive spring seasons and generation 4 during the autumn season in year 3. Each pure line contained two groups of selected and random (control) groups. Comparisons included the effect …

Slow Swimming, Fast Strikes: Effects Of Feeding Behavior On Scaling Of Anaerobic Metabolism In Epipelagic Squid, Lloyd A. Trueblood, Brad A. Seibel

Biological Sciences Faculty Publications

Many pelagic fishes engage prey at high speeds supported by high metabolic rates and anaerobic metabolic capacity. Epipelagic squids are reported to have among the highest metabolic rates in the oceans as a result of demanding foraging strategies and the use of jet propulsion, which is inherently inefficient. This study examined enzymatic proxies of anaerobic metabolism in two species of pelagic squid, Dosidicus gigas and Doryteuthis pealeii (Lesueur 1821), over a size range of six orders of magnitude. We hypothesized that activity of the anaerobically poised enzymes would be high and increase with size as in ecologically similar fishes. In …

Partial Pathogen Protection By Tick-Bite Sensitization And Epitope Recognition In Peptide-Immunized Hla Dr3 Transgenic Mice, Wendy M. C. Shattuck, Megan C. Dyer, Joe Desrosiers, Loren D. Fast, Frances E. Terry, William D. Martin, Leonard Moise, Anne S. De Groot, Thomas N. Mather

Institute for Immunology and Informatics Faculty Publications

Ticks are notorious vectors of disease for humans, and many species of ticks transmit multiple pathogens, sometimes in the same tick bite. Accordingly, a broad-spectrum vaccine that targets vector ticks and pathogen transmission at the tick/host interface, rather than multiple vaccines against every possible tickborne pathogen, could become an important tool for resolving an emerging public health crisis. The concept for such a tick protective vaccine comes from observations of an acquired tick resistance (ATR) that can develop in non-natural hosts of ticks following sensitization to tick salivary components. Mice are commonly used as models to study immune responses to …

Peptide-Pulsed Dendritic Cells Induce The Hepatitis C Viral Epitope-Specific Responses Of Naïve Human T Cells, Sasmita Mishra, Phyllis T. Losikoff, Alyssa A. Self, Frances Terry, Matthew T. Ardito, Ryan Tassone, William D. Martin, Anne S. De Groot, Stephen H. Gregory

Institute for Immunology and Informatics Faculty Publications

Hepatitis C virus (HCV) is a major cause of liver disease. Spontaneous resolution of infection is associated with broad, MHC class I- (CD8⁺) and class II-restricted (CD4⁺) T cell responses to multiple viral epitopes. Only 20% of patients clear infection spontaneously, however, most develop chronic disease. The response to chemotherapy varies; therapeutic vaccination offers an additional treatment strategy. To date, therapeutic vaccines have demonstrated only limited success in clinical trials. Vector-mediated vaccination with multi-epitope-expressing DNA constructs provides an improved approach. Highly-conserved, HLA-A2-restricted HCV epitopes and HLA-DRB1-restricted immunogenic consensus sequences (ICS, each composed of multiple overlapping and …

Tregitope: Immunomodulation Powerhouse, Leslie Cousens, Nader Najafian, William D. Martin, Anne S. De Groot

Institute for Immunology and Informatics Faculty Publications

IVIG is frequently used in the ‘pre-conditioning’ regimens for higher risk transplants; its effects are attributed in part to induction of Tregs. We have identified regulatory T cell (Treg) epitopes, now known as Tregitopes, in IgG, the main component of intravenous immunoglobulin therapy (IVIg). Tregitopes provide one explanation for the expansion and activation of Treg cells following IVIg treatment. Tregitopes are peptides that exhibit high affinity binding to multiple human HLA Class II DR; they are conserved across IgG isotypes and mammalian species. In vitro and in vivo, for human PBMC and in animal models, Tregitopes activate Tregs. Studies to …