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Application Of Polychlorinated Biphenyl Signatures For Environmental Fingerprinting, David Peter Megson
Application Of Polychlorinated Biphenyl Signatures For Environmental Fingerprinting, David Peter Megson
School of Geography, Earth and Environmental Sciences Theses
Polychlorinated Biphenyls (PCBs) are a group of 209 ‘man-made’ chlorinated organic compounds that were widely used in the 20th century for a variety of industrial uses. PCBs were first commercially produced in the 1929 and were manufactured until the 1980s when their use was phased out due to environmental and human health risks. However, due to their widespread use and persistence they are ubiquitous in the environment and remain a contaminant of concern. The structural properties that determine the persistence of PCBs in humans were therefore elucidated by statistical analysis of data from the National Health and Nutrition Examination Survey …
The Biogeochemistry Of Iron, Zinc And Cobalt In The Atlantic Ocean: The Atlantic Meridional Transect And Uk Geotraces Sections, Neil Wyatt
School of Geography, Earth and Environmental Sciences Theses
Between 40 % and 50 % of the Earth’s primary production occurs in marine environments, primarily by phytoplankton. The trace metal micronutrients iron, zinc and cobalt are known to exert a significant biological control on phytoplankton productivity by serving as essential active centres in enzymatic processes such as inorganic carbon, nitrogen and phosphorus acquisition. The distributions and biogeochemistries of iron, zinc and cobalt therefore, have the potential to impact upon the global carbon cycle and hence climate. This research involves investigations into the biogeochemical cycling of iron, zinc and cobalt in the Atlantic Ocean. Iron measurements were conducted during October …
Development Of A Nigerian Fermented Maize Food “Akamu” As A Functional Food, Patience Chisa Obinna-Echem
Development Of A Nigerian Fermented Maize Food “Akamu” As A Functional Food, Patience Chisa Obinna-Echem
Other Faculty of Science and Engineering Theses
Akamu is a lactic acid bacteria fermented cereal-based food that complements infant diets in most African countries. Uncontrolled fermentation increases the variability in quality and safety of akamu. This study was aimed at the controlled fermentation of akamu with selected lactic acid bacteria (LAB), investigation of the probiotic potential of the LAB and the effect of variation in production method on the product quality and sensory properties. PCR-DGGE analysis of traditional akamu samples revealed LAB community dominated by Lactobacillus fermentum, L. plantarum, L. delbrueckii subsp. bulgaricus and L. helveticus. Isolated yeasts were Candida tropicalis, C. albicans, Clavispora lusitaniae and Saccharomyces …
Source Tracking Of Faecal Indicator Bacteria Of Human Pathogens In Bathing Waters; An Evaluation And Development, Khwam Reissan Hussein
Source Tracking Of Faecal Indicator Bacteria Of Human Pathogens In Bathing Waters; An Evaluation And Development, Khwam Reissan Hussein
Other Faculty of Science and Engineering Theses
Bacterial water pollution is a significant problem because it is associated with reduction in the ‘quality’ of water systems with a potential impact on human health. Faecal indicator bacteria (FIB) are usually used to monitor the quality of water, and to indicate the presence of pathogens in water bodies. However, enumeration alone does not enable identification of the precise origin of these pathogens. This study aimed to monitor the quality of bathing water and associated fresh water in and out of the ‘bathing season’ in the UK, and to evaluate the use of microbial source tracking (MST) such as the …
Quality And Shelf Life Of Pita And Tandoori Breads Supplemented With Three Novel Functional Ingredients, Yaseen Galali
Quality And Shelf Life Of Pita And Tandoori Breads Supplemented With Three Novel Functional Ingredients, Yaseen Galali
Other Faculty of Science and Engineering Theses
Abstract: The interest for functional foods rich in dietary fibre (DF) and low in glycaemic response (GR) is steadily increasing. This is because DF could reduce GR of food products and protect and restrict diet-associated diseases which have become common in society. But DF rich products are not always high in quality and palatability. Therefore, more fibre-rich functional products need to be designed and developed with good quality, palatability and longer shelf life. A response surface methodology (RSM) was used to develop both pita and Tandoori breads rich in DF with good quality, palatability and longer shelf life. So, a …
Blinking In Human Communicative Behaviour And It's Reproduction In Artificial Agents, Christopher Colin Ford
Blinking In Human Communicative Behaviour And It's Reproduction In Artificial Agents, Christopher Colin Ford
Other Faculty of Science and Engineering Theses
A significant year-on-year rise in the creation and sales of personal and domestic robotic systems and the development of online embodied communicative agents (ECAs) has in parallel seen an increase in end-users from the public domain interacting with these systems. A number of these robotic/ECA systems are defined as social, whereby they are physically designed to resemble the bodily structure of a human and behaviorally designed to exist within human social surroundings. Their behavioural design is especially important with respect to communication as it is commonly stated that for any social robotic/ECA system to be truly useful within its role, …
Comparison Of Behaviour Exhibited By Horses Ridden In Conventional Bitted And Bitless Bridles, Rose Margaret Scofield
Comparison Of Behaviour Exhibited By Horses Ridden In Conventional Bitted And Bitless Bridles, Rose Margaret Scofield
Other Faculty of Science and Engineering Theses
The bitless bridle has been advocated as an alternative to traditional bitted bridles in many equestrian disciplines in order to avoid bit injury and distress that has been reported in previous research. Anecdotal observations and lay publications suggest that the bitless bridle has become very popular amongst riders and horse owners in the United Kingdom. The bitless bridle has been compared favourably to the use of the bit in foundation training with improved performance in bridling, long-reining and riding reported for some horses in the bitless sample group. Similarly, improved dressage test scores and comments by a judge have been …
Plant Tissue Culture And Artificial Seed Production Techniques For Cauliflower And Their Use To Study Molecular Analysis Of Abiotic Stress Tolerance, Hail Rihan
Other Faculty of Science and Engineering Theses
A protocol for cauliflower micro-propagule production was developed and optimised for both micropropagation and artificial seed production techniques using meristematic tissues from cauliflower curd. All steps in the protocol were empirically optimised including: blending, sieving, culture methods, liquid culture media composition and plant growth regulator combinations and concentrations. The cost of the micro-propagules could be reduced by as much as 50% on the initial costings reported previously since treatments doubled the number of microshoots produced per culture unit. The research confirmed the suitability of cauliflower microshoots to be encapsulated as artificial seeds and an effective protocol for microshoot encapsulation was …
The Role Of Cd38 Expression On Nad Levels And Cell Physiology In A Leukaemia Model, Zainab N Al-Abady
The Role Of Cd38 Expression On Nad Levels And Cell Physiology In A Leukaemia Model, Zainab N Al-Abady
Other Faculty of Science and Engineering Theses
CD38 is a transmembrane glycoprotein with both ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase activities; it is also known as a cell surface receptor. CD38 utilizes NAD(P) as a substrate to produce the second messengers, Nicotinic acid adenine dinucleotide phosphate (NAADP) and Cyclic adenosine diphosphate ribose (cADPR). CD38 has been implicated in several diseases. For instance, in chronic lymphocytic leukemia (CLL), it is known as a poor prognostic marker and as a disease modifier. Also, abundant data are available on the receptor functions of CD38 in CLL. However, the aim of the work described in this thesis was to investigate the enzymatic functions …
Development And Characterisation Of A Three-Dimensional In Vitro Hepatic Fish Model To Investigate Xenobiotic Metabolism, Matthew Graham Joesph Baron
Development And Characterisation Of A Three-Dimensional In Vitro Hepatic Fish Model To Investigate Xenobiotic Metabolism, Matthew Graham Joesph Baron
Other Faculty of Science and Engineering Theses
Regulatory and academic studies use large numbers of fish annually. The use of fish primary cells offers an in vitro alternative for the assessment of chemical toxicity and the evaluation of environmental samples in ecotoxicology. Their uses however are not without limitations which includes short culture periods (i.e. longevity and loss of organ-specific functionality over time. Three-dimensional (3-D spheroid) technology is now established for in vitro mammalian toxicity studies and offers significant advantages for environmental applications in a model fish species. This thesis reports development of a reproducible six-well plate, gyratory-mediated method for rainbow trout (Oncorhynchus mykiss) hepatocyte spheroid culture …
Multiplicity Of Viral Infection In Brown Algae, Kim Stevens
Multiplicity Of Viral Infection In Brown Algae, Kim Stevens
Other Faculty of Science and Engineering Theses
Brown algae are important primary producers and habitat formers in coastal environments and are believed to have evolved multicellularity independently of the other eukaryotes. The phaeoviruses that infect them form a stable lysogenic relationship with their host via genome integration, but have only been extensively studied in two genera: Ectocarpus and Feldmannia. In this study I aim to improve our understanding of the genetic diversity, host range and distribution of phaeoviruses. Sequencing and phylogenetic analysis of amplified fragments of three core phaeoviral genes (encoding major capsid protein (MCP), DNA polymerase and superfamily III helicase) of phaeovirus infected algae confirmed the …
The Integration Of Coastal Flooding Into An Arcflood Data Model, Alison Heidi Nock
The Integration Of Coastal Flooding Into An Arcflood Data Model, Alison Heidi Nock
Other Faculty of Science and Engineering Theses
With the impact of global climate change, the speedy, intelligent and accessible dissemination of coastal flood predictions from a number of modelling tools at a range of temporal and spatial scales becomes increasingly important for policy decision makers. This thesis provides a novel approach to integrate the coastal flood data into an ArcFLOOD data model to improve the analysis, assessment and mitigation of the potential flood risk in coastal zones. This novel methodology has improved the accessibility, dissemination and visualisation of coastal flood risk. The results were condensed into spatial information flows, data model schematic diagrams and XML schema for …
Between Selves And Others: Exploring Strategic Approaches Within Visual Art, Teresa Chen
Between Selves And Others: Exploring Strategic Approaches Within Visual Art, Teresa Chen
Other Faculty of Arts, Humanities and Business Theses
This body of research investigates how visual artists express ideas or meanings about Otherness and issues of belonging in their art. The focus of this study is on women artists with an (East) Asian diasporic background; however, the context of the inquiry includes other American and European artists of various cultural backgrounds. A further aim is to explore the artistic strategies and the historical circumstances of the works as well as to understand the theoretical correlations. The author of this study is a visual artist who has been exploring similar issues in her own artistic practice. In order to examine …
Facing Experience: A Painter's Canvas In Virtual Reality, Margaret Dolinsky
Facing Experience: A Painter's Canvas In Virtual Reality, Margaret Dolinsky
Other Faculty of Arts, Humanities and Business Theses
This research investigates how shifts in perception might be brought about through the development of visual imagery created by the use of virtual environment technology. Through a discussion of historical uses of immersion in art, this thesis will explore how immersion functions and why immersion has been a goal for artists throughout history. It begins with a discussion of ancient cave drawings and the relevance of Plato’s Allegory of the Cave. Next it examines the biological origins of “making special.” The research will discuss how this concept, combined with the ideas of “action” and “reaction,” has reinforced the view that …
Perceiving Voids: Memory And Sight Afflictions In Contemporary Cinema, Francesco Marineo
Perceiving Voids: Memory And Sight Afflictions In Contemporary Cinema, Francesco Marineo
Other Faculty of Arts, Humanities and Business Theses
My thesis focuses on the perceptive afflictions caused by alteration of the normal biological functioning of sight and memory. These afflictions are related to the redefinition and disgregation of the classical and postclassical cinematographic characters, and affect cinematographic language, establishing a dialectical relation with the filmic image that contaminates our spectatorial perception. In the first chapter I propose a different reading of a few moments in film history, turning points in which a modification of the ordinary sensorial patterns has been introduced. From the German Expressionism to the late authorial experiments of the 60s, there is a sort of hidden …
Discourses Pertaining To, And Lived Experiences Of, 'Maternal Obesity' (Body Mass Index (Bmi) ≥ 30) And Gestational Diabetes Mellitus/Type Two Diabetes Mellitus In The Pregnancy And Post-Birth Period., Rachel Juliet Jarvie
Other Faculty of Arts, Humanities and Business Theses
This thesis reports on a qualitative exploration of the experiences of 30 women designated as ‘high risk’ due to the co-existence of ‘maternal obesity’ (BMI ≥ 30) and Gestational Diabetes Mellitus (GDM)/Type Two Diabetes Mellitus (T2DM) in pregnancy. This is examined in the context of medico-scientific/public health/ popular media discourses pertaining to ‘maternal obesity’/GDM/T2DM in pregnancy. ‘Maternal obesity’/GDM/T2DM in pregnancy are increasingly prevalent and clinically associated in manifold ways. Increasing prevalence is linked to the ‘global epidemic’ of ‘obesity’/diabetes: now commonly referred to as ‘diabesity’. Current biomedical knowledge asserts ‘maternal obesity’ and diabetes (‘maternal diabesity’) synergise in causing adverse pregnancy …
An Investigation Of The Coherence Of Instrumental Accumulation, Using A Sculptural Methodology, Nicola Ann Gilmour
An Investigation Of The Coherence Of Instrumental Accumulation, Using A Sculptural Methodology, Nicola Ann Gilmour
Other Faculty of Arts, Humanities and Business Theses
This thesis questions the coherence of modes of fabrication that introduce materials to a living context, in forms that resist the processes of biological change. In doing so this project explores the ideologies embedded in fabrication that have led to this current position. The implications of an accumulation of materials being recognized as an autonomous object, and treated as if they are detached from their environment are also expanded. The sculptural methodology used to undertake this investigation has used the feature of materiality and it’s behaviours, of both human fabrication and the living environment, as a means to explore processes …
The Multidimensional Depth Of The Image: Body-Environment-Artefact (A Philosophical Reflection For Graphic Design), Martyn Stuart Woodward
The Multidimensional Depth Of The Image: Body-Environment-Artefact (A Philosophical Reflection For Graphic Design), Martyn Stuart Woodward
School of Art, Design and Architecture Theses
The Multidimensional Depth of the Image: Body-Environment-Artefact Current discourses within cultural studies are re-iterating the limitations of language to adequately describe the affective domains of corporeality and materiality in the study of cultural artefacts. Within the discourse of graphic design, however, there remains an enduring focus placed upon models of language and communication to understand the meaning of designed materials. Rather than upholding a focus upon language, this thesis undertakes a theoretical investigation to extend the literature available to the discourse of graphic design to better understand how visual materials ‘come to mean’ within the experience of an embodied subject …
The Bioavailability And Assimilation Of Dietary Zinc In Rainbow Trout (Oncorhynchus Mykiss), Daniel James Leeming
The Bioavailability And Assimilation Of Dietary Zinc In Rainbow Trout (Oncorhynchus Mykiss), Daniel James Leeming
Other Faculty of Science and Engineering Theses
This study examines three possible methods for improving the digestibility and bioavailability of zinc to rainbow trout (Oncorhynchus mikiss). The first method was to examine the availability of the zinc utilisation from commonly used protein sources; the second was to assess the efficacy of the upstream use of an enzyme treatment of the raw materials; the third was to assess the use of organically complexed mineral supplements as opposed to the inorganic salts widely used at present. The first section indicated that the zinc from the soyabean meal was the most available (49.4%). The zinc digestibilities of the animal based …
The Effect Of Probiotics On Bacterial Human Skin Pathogens, Jasim Hussein Al-Attwani
The Effect Of Probiotics On Bacterial Human Skin Pathogens, Jasim Hussein Al-Attwani
Other Faculty of Science and Engineering Theses
Probiotic bacteria have been investigated in the prevention and treatment of various diseases and allergies. The current study was undertaken to determine the effect of eight probiotic Lactobacillus species against bacterial human skin pathogens using several techniques. Antimicrobial activity of lactobacilli against Staphylococcus aureus, Staphylococcus epidermidis, Pseudomonas aeruginosa and Propionibacterium acnes was evaluated using lactobacilli broth cultures (BCB) and cell free supernatant (CFS). Antimicrobial activity was significantly greater with BCB compared with CFS especially for Lactobacillus plantarum and Lactobacillus acidophilus. Lactobacilli and pathogen aggregation, biofilm formation and adhesion to keratin were assessed. L. casei and L. plantarum were selected for …
Cardiopulmonary Exercise Capacity And Preoperative Markers Of Inflammation, Pervez Sultan, Mark R. Edwards, Ana Gutierrez Del Arroyo, David Cain, J. Robert Sneyd, Richard Struthers, Gary Minto, Gareth L. Ackland
Cardiopulmonary Exercise Capacity And Preoperative Markers Of Inflammation, Pervez Sultan, Mark R. Edwards, Ana Gutierrez Del Arroyo, David Cain, J. Robert Sneyd, Richard Struthers, Gary Minto, Gareth L. Ackland
More Faculty of Health Research
Explanatory mechanisms for the association between poor exercise capacity and infections following surgery are underexplored. We hypothesized that aerobic fitness - assessed by cardiopulmonary exercise testing (CPET) - would be associated with circulating inflammatory markers, as quantified by the neutrophil-lymphocyte ratio (NLR) and monocyte subsets. The association between cardiopulmonary reserve and inflammation was tested by multivariable regression analysis with covariates including anaerobic threshold (AT) and malignancy. In a first cohort of 240 colorectal patients, AT was identified as the sole factor associated with higher NLR (P = 0.03) and absolute and relative lymphopenia (P = 0.01). Preoperative leukocyte subsets and …
The Impact Of Implicit Motives On The Business To Business Decision Making Process, Christian Chlupsa
The Impact Of Implicit Motives On The Business To Business Decision Making Process, Christian Chlupsa
Plymouth Business School Theses
Abstract THE IMPACT OF IMPLICIT MOTIVES ON THE BUSINESS TO BUSINESS DECISION MAKING PROCESS Christian Chlupsa The purpose of this thesis is to understand how implicit processes determine individual and organisational behaviour in the context of business to business (B2B) decision making. A broad literature review suggests that implicit cognition has a significantly more powerful influence than therefore assumed. The author calls for further research into the motivations driving professional behaviour in B2B situations as new study challenges the classical economic theory of the homo economicus, and focuses on the role of implicit motives as a possible driver. As a …
The Antibacterial Properties And Biocompatibility Of Silver And Hydroxyapatite Nanoparticles Coating On Dental Implants, Sanna Dara Hadi
The Antibacterial Properties And Biocompatibility Of Silver And Hydroxyapatite Nanoparticles Coating On Dental Implants, Sanna Dara Hadi
Other Faculty of Science and Engineering Theses
Introduction: Infection associated with dental implants is the most common cause of their failure. The aim of this study was to discover a new coating for dental implants that has a good antibacterial and antibiofilm activity against an early colonizer on implant surfaces: Streptococcus sanguinis. Furthermore, the coating should have a good biocompatibility with bone. Methods: Different coatings were prepared on the surface of titanium alloy (Ti4Al6V) discs. Silver, titanium dioxide, and hydroxyapatite nanoparticles, as well as hydroxyapatite microparticles were used to coat titanium discs. Three techniques were used to coat the titanium discs; (i) Solution deposition and heating for …
Somatic Embryogenesis And Cryopreservation Of Cauliflower (Brassica Oleracea Var. Botrytis), Magda Al Shamari
Somatic Embryogenesis And Cryopreservation Of Cauliflower (Brassica Oleracea Var. Botrytis), Magda Al Shamari
Other Faculty of Science and Engineering Theses
Abstract Successful efficient whole cauliflower plant regeneration via somatic embryogenesis from root derived callus tissue was achieved. The research confirmed for the first time the capability of mass production of cauliflower somatic embryos through the indirect pathway. The best callus induction and proliferation was on semi solid Murashige and Skoog (MS) medium supplemented with 2, 4-D at 0.15 mg L-1 and Kinetin at 0.1 mg L-1 and 3% sucrose. The response of different explant types (cotyledon, hypocotyls and root) through callus induction and subsequent culture was determined. The best period for subsequent callus culture was 21 days. Continuous immersion in …
Effect Of Dietary Β-Glucan Supplementation On Growth, Intestinal Functionality And Disease Progression In Selected Cyprinids (Cyprinus Carpio And Danio Rerio), Holger Kühlwein
Other Faculty of Science and Engineering Theses
Three experiments were conducted to investigate the effects of dietary supplementation of a ß-(1,3)(1,6)-D-glucan derived from the yeast Saccharomyces cerevisiae, on growth performance parameters and intestinal functionality in mirror carp (Cyprinus carpio L.) and on progression of a chronic, pre-existing mycobacteriosis in zebrafish (Danio rerio). The 1st experiment revealed that mirror carp fed diets containing 1% and 2% ß-glucan showed significant improvements in growth performance compared to fish fed both the control and the 0.1% ß-glucan containing diet. Equally, fish fed diets supplemented with 1% and 2% ß-glucan displayed significantly higher infiltration of leukocytes into the epithelial layer of in …
Computational Study Of The Mechanisms Underlying Oscillation In Neuronal Locomotor Circuits, Robert Merrison-Hort
Computational Study Of The Mechanisms Underlying Oscillation In Neuronal Locomotor Circuits, Robert Merrison-Hort
Other Faculty of Science and Engineering Theses
In this thesis we model two very different movement-related neuronal circuits, both of which produce oscillatory patterns of activity. In one case we study oscillatory activity in the basal ganglia under both normal and Parkinsonian conditions. First, we used a detailed Hodgkin-Huxley type spiking model to investigate the activity patterns that arise when oscillatory cortical input is transmitted to the globus pallidus via the subthalamic nucleus. Our model reproduced a result from rodent studies which shows that two anti-phase oscillatory groups of pallidal neurons appear under Parkinsonian conditions. Secondly, we used a population model of the basal ganglia to study …
Final Report To Ospar Of The Joint Ospar/Ices Ocean Acidification Study Group (Sgoa), E Mcgovern, Jm Hall-Spencer
Final Report To Ospar Of The Joint Ospar/Ices Ocean Acidification Study Group (Sgoa), E Mcgovern, Jm Hall-Spencer
School of Biological and Marine Sciences
No abstract provided.
Differential Responses Of Emergent Intertidal Coral Reef Fauna To A Large-Scale El-Niño Southern Oscillation Event: Sponge And Coral Resilience., Francisco Kelmo, James J. Bell, Simone Souza Moraes, Rilza Da Costa Tourinho Gomes, Eduardo Mariano-Neto, Martin J. Attrill
Differential Responses Of Emergent Intertidal Coral Reef Fauna To A Large-Scale El-Niño Southern Oscillation Event: Sponge And Coral Resilience., Francisco Kelmo, James J. Bell, Simone Souza Moraes, Rilza Da Costa Tourinho Gomes, Eduardo Mariano-Neto, Martin J. Attrill
School of Biological and Marine Sciences
There is a paucity of information on the impacts of the 1997-8 El Niño event and subsequent climatic episodes on emergent intertidal coral reef assemblages. Given the environmental variability intertidal reefs experience, such reefs may potentially be more resilient to climatic events and provide important insights into the adaptation of reef fauna to future ocean warming. Here we report the results of a 17-year (1995-2011) biodiversity survey of four emergent coral reef ecosystems in Bahia, Brazil, to assess the impact of a major El Niño event on the reef fauna, and determine any subsequent recovery. The densities of two species …
The Impact Of Changing Surface Ocean Conditions On The Dissolution Of Aerosol Iron, Matthew P. Fishwick, Peter N. Sedwick, Maeve C. Lohan, Paul J. Worsfold, Kristen N. Buck, Thomas M. Church, Simon J. Ussher
The Impact Of Changing Surface Ocean Conditions On The Dissolution Of Aerosol Iron, Matthew P. Fishwick, Peter N. Sedwick, Maeve C. Lohan, Paul J. Worsfold, Kristen N. Buck, Thomas M. Church, Simon J. Ussher
School of Geography, Earth and Environmental Sciences
The proportion of aerosol iron (Fe) that dissolves in seawater varies greatly and is dependent on aerosol composition and the physicochemical conditions of seawater, which may change depending on location or be altered by global environmental change. Aerosol and surface seawater samples were collected in the Sargasso Sea and used to investigate the impact of these changing conditions on aerosol Fe dissolution in seawater. Our data show that seawater temperature, pH, and oxygen concentration, within the range of current and projected future values, had no significant effect on the dissolution of aerosol Fe. However, the source and composition of aerosols …
Combining Motion Analysis And Microfluidics--A Novel Approach For Detecting Whole-Animal Responses To Test Substances., Tabitha S. Rudin-Bitterli, Oliver Tills, John I. Spicer, Phil F. Culverhouse, Eric M. Wielhouwer, Michael K. Richardson, Simon D. Rundle
Combining Motion Analysis And Microfluidics--A Novel Approach For Detecting Whole-Animal Responses To Test Substances., Tabitha S. Rudin-Bitterli, Oliver Tills, John I. Spicer, Phil F. Culverhouse, Eric M. Wielhouwer, Michael K. Richardson, Simon D. Rundle
School of Biological and Marine Sciences
Small, early life stages, such as zebrafish embryos are increasingly used to assess the biological effects of chemical compounds in vivo. However, behavioural screens of such organisms are challenging in terms of both data collection (culture techniques, drug delivery and imaging) and data evaluation (very large data sets), restricting the use of high throughput systems compared to in vitro assays. Here, we combine the use of a microfluidic flow-through culture system, or BioWell plate, with a novel motion analysis technique, (sparse optic flow - SOF) followed by spectral analysis (discrete Fourier transformation - DFT), as a first step towards automating …