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Development And Application Of A Safety Index For Transit Stops: A Case Study From Las Vegas, Nevada, Rithesh Shivuni Dec 2022

Development And Application Of A Safety Index For Transit Stops: A Case Study From Las Vegas, Nevada, Rithesh Shivuni

UNLV Theses, Dissertations, Professional Papers, and Capstones

This research developed a methodology to evaluate safety proximate to transit stops through a Safety Index, identified key factors associated with fatal crashes proximate to bus transit stops, and illustrated the applications of these using a case study based on the Las Vegas metropolitan area in Nevada, USA. The methodology is based on motor vehicle crashes that involve pedestrians recorded in the proximity of transit stops, and the severity of the crash outcomes. It focused on crashes which involved pedestrians that occurred within walking distances of individual transit stops. The severity of the crash outcomes was classified based on the …


Jiapi: A Type Checker Generator For Statically Typed Languages, Benjamin Cisneros Merino Dec 2022

Jiapi: A Type Checker Generator For Statically Typed Languages, Benjamin Cisneros Merino

UNLV Theses, Dissertations, Professional Papers, and Capstones

Type systems are a key characteristic in the context of the study of programming languages. They frequently offer a simple, intuitive way of expressing and testing the fundamental structure of programs. This is especially true when types are used to provide formal, machine-checked documentation for an implementation. For example, the absence of type errors in code prior to execution is what type systems for static programming languages are designed to assure, and in the literature, type systems that satisfy this requirement are referred to as sound type systems. Types also define module interfaces, making them essential for achieving and maintaining …


Examination Of The Relationship Between Loneliness And Binge Eating Symptoms, Kimberly Stevens Dec 2022

Examination Of The Relationship Between Loneliness And Binge Eating Symptoms, Kimberly Stevens

UNLV Theses, Dissertations, Professional Papers, and Capstones

Loneliness is implicated in several mental health conditions and may also be linked to eating pathology, like binge eating symptoms (e.g., loss-of-control eating). However, the pathways by which loneliness might be associated with binge eating symptoms are less well understood. Preliminary evidence suggests perceived stress may be a key explanatory pathway. Specifically, perceived stress mediates the impact of loneliness on other mental health outcomes and is associated with increased risk of binge eating symptoms. This study investigated relationships between loneliness, perceived stress, and binge eating symptoms and tested whether heightened levels of binge eating was accounted for by increases in …


The Impact Of Alzheimer Disease On Semantic Knowledge, Maileen G. Ulep Dec 2022

The Impact Of Alzheimer Disease On Semantic Knowledge, Maileen G. Ulep

UNLV Theses, Dissertations, Professional Papers, and Capstones

The research furthers the understanding of the impact of Alzheimer disease (AD) on cognition and the organization of semantic knowledge in the brain, which might contribute to the development of diagnostic and staging tools, and interventions to palliate cognitive deficits. The disruption of semantic knowledge in AD is well documented in the literature. Much of the existing research focuses on the general impact AD has on semantic knowledge. This study explores the impact of AD on specific domains of knowledge, chiefly, living kinds and artifacts, critical to ordinary functioning. The content, organization and structure of the investigated domains of knowledge …


College Students’ Attitudes Toward Condom Use In Pornography: Associations With Sexual History, Sexually Transmitted Infections, And Pornography Use, Bailey M. Way Dec 2022

College Students’ Attitudes Toward Condom Use In Pornography: Associations With Sexual History, Sexually Transmitted Infections, And Pornography Use, Bailey M. Way

UNLV Theses, Dissertations, Professional Papers, and Capstones

The depiction of condomless sex in pornography has significant public health implications for adult film (pornography) actors and potentially for its consumers. Previous research suggests that most consumers of pornography are in favor of condom use in pornography; however, available research on attitudes toward condom use in pornography has surveyed mostly White, male, American undergraduate students. Currently, there is a need to explore viewers’ attitudes toward condom use in pornography among women and racially/ethnically diverse individuals. The current study investigated United States (US) college students’ attitudes toward condom use in pornography using the Pornography Actors’ Condom Use Attitudes Questionnaire (PACUAQ). …


Racialized Places: Community Development Within The Historic Westside Of Las Vegas, Camisha Fagan Dec 2022

Racialized Places: Community Development Within The Historic Westside Of Las Vegas, Camisha Fagan

UNLV Theses, Dissertations, Professional Papers, and Capstones

My paper examines the history of the Historic Westside (HWS) in Las Vegas, Nevada, and how community development decisions made by city officials have led to the marginalization of this area. Specifically, my paper examines the impact of discriminatory policies and practices on the HWS, including inadequate infrastructure, racist housing policies, and economic abandonment. In doing so, I draw on a range of sources, including archival materials, interviews, and secondary literature, to provide a comprehensive analysis of the social, economic, and political factors that have contributed to the marginalization of the HWS. My paper focuses on four key development events …


Customer Willingness To Pay For All-Inclusive Resorts: A Choice-Based Conjoint Analysis, Liheng Zhang Dec 2022

Customer Willingness To Pay For All-Inclusive Resorts: A Choice-Based Conjoint Analysis, Liheng Zhang

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation examined customers’ willingness to pay for all-inclusive resorts using choice theory, the theory of bundling, and prospect theory. This dissertation also contributed to the current research by determining (a) the attributes that affect customers’ willingness to pay for an all-inclusive resort and (b) whether a reference price will affect customers’ willingness to pay. We also take into account the effect of the COVID-19 pandemic on customers and whether the pandemic will influence customers’ purchase behavior at this particular time. The choice-based conjoint analysis results showed that all eleven attributes—price, hotel star rating, type of room, location, food options, …


A Control Integrated Circuit For A Hysteretic Flyback Power Converter, Armani Alvarez Dec 2022

A Control Integrated Circuit For A Hysteretic Flyback Power Converter, Armani Alvarez

UNLV Theses, Dissertations, Professional Papers, and Capstones

This Thesis reports the design, simulation, and layout of a switching power supply controller chip for a fly-back switching power supply. The controller chip was designed using ON’s Semiconductor C5 process. The resulting switching power supply using the controller chip consists of using various off-chip components such as a power MOSFET, transformer, and capacitors. The main components used in the integrated circuit include a bandgap reference voltage circuit which outputs a reference voltage of 1.25V, a ring oscillator having a nominal frequency of 5MHz, a high-gain comparator, and a buffer that can drive a 5pF capacitor. Under normal simulation conditions …


Activity-Based Intervention And One-To-One Correspondence For Children With Developmental Delays, Cyndy Anang Dec 2022

Activity-Based Intervention And One-To-One Correspondence For Children With Developmental Delays, Cyndy Anang

UNLV Theses, Dissertations, Professional Papers, and Capstones

PreK children with developmental delays in inclusive classrooms need access to math interventions helps them develop foundational math skills that are needed to succeed during their K-12 education. Research is significantly lacking that addresses increasing math skills for children with disabilities in PreK classrooms. The purpose of this study was to examine how using activity-based intervention (ABI) increases one-to-one correspondence for children with disabilities in an inclusive classroom. A single subject multiple probe design across participants was implemented to embed one-to-one correspondence math goals into daily routines and preferred activities. The participants of the study were two 3–5-year-old children categorized …


Predicting Student Success In A K-12 Online School, Andrea Connolly Dec 2022

Predicting Student Success In A K-12 Online School, Andrea Connolly

UNLV Theses, Dissertations, Professional Papers, and Capstones

Given the rapid growth of K-12 online learning, research is needed in the effective identification of at-risk students so that administrators and teachers can develop appropriate supports and interventions. The purpose of this research was to determine if student success in an online course could be predicted for English Learners (EL) using identifiable student characteristics and traits. This study looked at the student traits of behavior, structure, and demographics to determine if they could be used as predictive variables for successful course completion in a K-12 online course. The behavior characteristics were measured by a survey tool and were identified …


Exploring The Role Of Epistemic Cognition And Self-Efficacy In The Career Decision-Making Processes Of College Students: An Explanatory Sequential Study, Raelynn Frazier Dec 2022

Exploring The Role Of Epistemic Cognition And Self-Efficacy In The Career Decision-Making Processes Of College Students: An Explanatory Sequential Study, Raelynn Frazier

UNLV Theses, Dissertations, Professional Papers, and Capstones

College students are asked to make critical decisions about which academic major to choose and subsequently to make specific career decisions. Students come to college with a myriad of different skill sets, backgrounds, developmental levels, cognitive processes, familial support, and levels of self-efficacy that may influence how they make these decisions. The purpose of this study was to explore the role that epistemic cognition and self-efficacy may play in the career decision-making processes of college students utilizing a mixed methods approach. Results of this study indicate that there are internal and external influences that impact college students’ career decision-making processes …


Technology-Based Self-Management Of Initiations For Students With Autism Spectrum Disorder And Low Communication Who Participate In An Alternate Assessment, Amelia Fuqua Dec 2022

Technology-Based Self-Management Of Initiations For Students With Autism Spectrum Disorder And Low Communication Who Participate In An Alternate Assessment, Amelia Fuqua

UNLV Theses, Dissertations, Professional Papers, and Capstones

Self-management as a behavioral intervention has been used with students who have autism spectrum disorder for a variety of behavioral targets, including communication. Research has been conducted on self-management of communication initiations for students who have autism spectrum disorder in primary grades through high school. The process of self-management includes monitoring one’s own behavior to change that behavior. Currently, there are no studies using technology with self-management for the initiations of students with autism spectrum disorder and low communication who also participate in an alternate assessment. The purpose of this study was a systematic replication of Newman and Ten Eyck …


Exploring The Intersection Of Stress, Resiliency, And Relationship With Teacher Among High School Students With Emotional And Behavioral Disorders, Scotia Hammond Dec 2022

Exploring The Intersection Of Stress, Resiliency, And Relationship With Teacher Among High School Students With Emotional And Behavioral Disorders, Scotia Hammond

UNLV Theses, Dissertations, Professional Papers, and Capstones

Experiencing stress at high levels during pivotal developmental years can have a detrimental impact on adolescents’ overall development and well-being (Rahdar & Galvan, 2014; Romeo, 2013). Extensive research has shown the positive effects a strong student-teacher relationship has on students’ resiliency to counteract the negative effects of stress (Akin & Radford, 2018; Johnson, 2008; Masten et al., 2008; Mulloy, 2011; Sanders et al., 2016; Stewart & Sun, 2004). Unfortunately, these studies rarely include students with emotional and behavioral disorders (EBD). Students with EBD are more likely to have higher levels of stress, lower levels of resilience and often struggle forming …


Easing The Middle To High School Transition: An Evaluation Of The Gold Program, Leilani Shive Dec 2022

Easing The Middle To High School Transition: An Evaluation Of The Gold Program, Leilani Shive

UNLV Theses, Dissertations, Professional Papers, and Capstones

Some effective components of effective transition programs include using a freshman academy or school-within-a-school model, teacher and peer mentoring and summer bridge programs. This study analyzed the effectiveness of the Gold Program, a middle to high school transition program for at-risk youth in a large urban high school in the southwestern United States. This program incorporated those components in addition to several others, such as: parent involvement, career and technical education, blended learning, behavior interventions, and Air Force Junior Reserve Officer Training Corps (AFJROTC). Individual components of the Gold Program were chosen to meet the four S’s of Schlossberg’s transition …


Student Device Usage, Learning Management System Tool Usage, And Self-Regulated Learning, Nancy Webb Dec 2022

Student Device Usage, Learning Management System Tool Usage, And Self-Regulated Learning, Nancy Webb

UNLV Theses, Dissertations, Professional Papers, and Capstones

Self-regulated learning (SRL) is crucial to student success in online learning environments. Students enrolled in online courses at higher education institutions access courses through the learning management system (LMS) to complete coursework, interact with instructors and peers using various learning management system tools and multiple technological devices. Researchers frequently rely on surveys to identify how students self-regulate, but students often are not honest or over or under-estimate their SRL skills. Learning management system data analytics have recently provided opportunities to examine the frequencies and sequences of how learnings engage with tools and content in the online learning environment. The purpose …


Focus On Top Tier 2.0: Research, Scholarship, And Creative Activity, University Of Nevada, Las Vegas Nov 2022

Focus On Top Tier 2.0: Research, Scholarship, And Creative Activity, University Of Nevada, Las Vegas

Focus on Top Tier 2.0

UNLV is proud to be designated a Carnegie R1 institution, and our focus is framed broadly to include not only research, but scholarship and creative activity, speaking to the depth and breadth of programming we offer. We seek to increase innovation and excellence in research, with multiple interdisciplinary opportunities that further support scholarly and creative activity. Our efforts enhance student engagement opportunities and align program experiences with strategic career pathways.


Teacher Salaries In The Mountain West, 2019 - 2021, Corryn Richardson, Joshua Padilla, Hira Ahmed, Zachary Billot, Caitlin J. Saladino, William E. Brown Jr. Nov 2022

Teacher Salaries In The Mountain West, 2019 - 2021, Corryn Richardson, Joshua Padilla, Hira Ahmed, Zachary Billot, Caitlin J. Saladino, William E. Brown Jr.

K-12 Education

This fact sheet examines state-level data of teacher salary benchmarks from the National Education Association, a labor union representing public school teachers and other education faculty in the U.S. Data are presented on average teacher salaries, average teacher starting salaries, and teacher pay penalties in the Mountain West (Arizona, Colorado, Nevada, New Mexico, and Utah).


Strengthening The Southern Nevada Workforce Pipeline, Katie M. Gilbertson Nov 2022

Strengthening The Southern Nevada Workforce Pipeline, Katie M. Gilbertson

Policy Briefs and Reports

This report analyzes the Southern Nevada employment ecosystem by utilizing occupational clusters recognized by the U.S. Department of Labor. The first section examines occupations in the tourism industry using three occupational clusters: hospitality and leisure; arts, audio/video technology and communications; and the transportation, distribution, and logistics. Next, this report utilizes the Las Vegas Global Economic Alliance’s 2022 Workforce Blueprint to identify the top 15 in-demand occupations for Southern Nevada and occupational clusters. A case study of the MGM College Opportunity Program (COP) is presented to demonstrate an existing workforce training program that promotes upward mobility of leisure and hospitality employees …


The Impact Of High-Intensity Interval Training On Vascular Function In Adults: A Systematic Review And Meta-Analysis, Mousa Khalafi, Mohammad Hossein Sakhaei, Fatemeh Kazeminasab, Michael E. Symonds, Sara K. Rosenkranz Nov 2022

The Impact Of High-Intensity Interval Training On Vascular Function In Adults: A Systematic Review And Meta-Analysis, Mousa Khalafi, Mohammad Hossein Sakhaei, Fatemeh Kazeminasab, Michael E. Symonds, Sara K. Rosenkranz

Kinesiology and Nutrition Sciences Faculty Research

Aim: We performed a systematic review and meta-analysis to investigate the effects of high-intensity interval training (HIIT) compared with moderateintensity continuous training (MICT) or with no exercise (CON) on vascular function in adults who were free of cardiometabolic diseases and those with cardiometabolic diseases.

Methods: A search across three electronic databases including Scopus, PubMed, and Web of Science was conducted through February 2022 to identify the randomized trials evaluating HIIT vs. MICT and/or CON on vascular function as measured using brachial artery flow-mediated dilation (FMD) in adults. Separate analyses were conducted for HIIT vs. MICT and/or CON to calculate weighted …


Predicting Flossing Through The Application Of The Multi-Theory Model (Mtm) Of Health Behavior Change Among Minority Adolescents In The United States, Manoj Sharma, Kavita Batra, Ching-Chen Chen, Chia-Liang Dai, Ravi Batra, David Cappelli Nov 2022

Predicting Flossing Through The Application Of The Multi-Theory Model (Mtm) Of Health Behavior Change Among Minority Adolescents In The United States, Manoj Sharma, Kavita Batra, Ching-Chen Chen, Chia-Liang Dai, Ravi Batra, David Cappelli

Social and Behavioral Health Faculty Research

Adolescents from minority groups are particularly susceptible to poor oral hygiene behaviors, including lack of daily flossing. This cross-sectional study aimed to conduct an exploratory behavioral research to identify evidence-based (theory-based) approaches to promote flossing behavior among African American/Black and Latinx/Hispanic (minority) adolescents. A 39-item psychometrically valid web-based questionnaire was used to collect responses from a nationwide sample of minority adolescents aged 10–17 years residing in the United States. The data were analyzed using bivariate and multivariate statistical methods. Of 520 minority adolescents (260 African American/Black and 260 Latinx/Hispanic adolescents), the proportion of flossing was nearly equally split in the …


Macroscale Superdurable Superlubricity Achieved In Lubricant Oil Via Operando Tribochemical Formation Of Fullerene-Like Carbon, Jingjie Pan, Xinxin Gao, Chang Liu, Kan Zhang, Weitao Zheng, Changfeng Chen Nov 2022

Macroscale Superdurable Superlubricity Achieved In Lubricant Oil Via Operando Tribochemical Formation Of Fullerene-Like Carbon, Jingjie Pan, Xinxin Gao, Chang Liu, Kan Zhang, Weitao Zheng, Changfeng Chen

Physics & Astronomy Faculty Research

Friction and wear are ubiquitous in moving mechanical assemblies, causing energy and equipment loss and producing harmful emissions. Mitigation efforts have long relied on oil-based lubricants, but challenges remain in achieving concurrent ultralow friction and wear without oil additives. Here, we demonstrate that a dual-phase TiB2-MoS2 film lubricated by oil without additives exhibits superdurable superlubricity characterized by a vanishingly small friction coefficient (0.007) and ultralow wear rate (10−9 mm3N−1m−1) maintained in lubricants over an unprecedentedly long sliding distance without performance deterioration. We identify a tribochemical route for the decomposition of oil molecules catalytically activated by the TiB2-MoS2 film, leading to …


Air Quality In The Mountain West, 2018-2020, Zachary Billot, Zachary Walusek, Caitlin J. Saladino, William E. Brown Jr. Nov 2022

Air Quality In The Mountain West, 2018-2020, Zachary Billot, Zachary Walusek, Caitlin J. Saladino, William E. Brown Jr.

Environment

This fact sheet examines the state of air quality, including ozone levels and particle pollution, throughout the Mountain West from 2018 to 2020. Data from the American Lung Association “State of the Air” report is used to determine the quality of air in the Mountain West states of Arizona, Colorado, Nevada, New Mexico, and Utah.


Digitally Predicting Protein Localization And Manipulating Protein Activity In Fluorescence Images Using 4d Reslicing Gan, Yang Jiao, Lingkun Gu, Yingtao Jiang, Mo Weng, Mei Yang Nov 2022

Digitally Predicting Protein Localization And Manipulating Protein Activity In Fluorescence Images Using 4d Reslicing Gan, Yang Jiao, Lingkun Gu, Yingtao Jiang, Mo Weng, Mei Yang

Electrical & Computer Engineering Faculty Research

While multi-channel fluorescence microscopy is a vital imaging method in biological studies, the number of channels that can be imaged simultaneously is limited by technical and hardware limitations such as emission spectra cross-talk. One solution is using deep neural networks to model the localization relationship between two proteins so that the localization of one protein can be digitally predicted. Furthermore, the input and predicted localization implicitly reflect the modeled relationship. Accordingly, observing the response of the prediction via manipulating input localization could provide an informative way to analyze the modeled relationships between the input and the predicted proteins.


Cutting Edge Technology Metros In The Mountain West, 2021, Sofia Takhtadjian, Elia Del Carmen Solano-Patricio, Caitlin J. Saladino, William E. Brown Jr. Nov 2022

Cutting Edge Technology Metros In The Mountain West, 2021, Sofia Takhtadjian, Elia Del Carmen Solano-Patricio, Caitlin J. Saladino, William E. Brown Jr.

Economic Development & Workforce

This fact sheet highlights data on cutting-edge technology metros in the Mountain West, as originally reported in the Commercial Cafe report, “Boulder Tops Silicon Valley as Most Cutting-Edge Metro in the U.S.” Of the top 30 cutting-edge metros in the nation, four are in the Mountain West: Boulder, CO (1st nationally); Denver, CO (7th nationally); Salt Lake City, UT (10th nationally); and Phoenix, AZ (27th nationally).


Pancreaticoduodenectomy In 11-Year-Old Male With A Non-Functional Pancreatic Neuroendocrine Tumor, Whitney Elks, Jessa Rose Li, Alexander Zajack, Lance Horner, Alan Ikeda, Charles St Hill, Michael Scheidler, Shirong Chang Nov 2022

Pancreaticoduodenectomy In 11-Year-Old Male With A Non-Functional Pancreatic Neuroendocrine Tumor, Whitney Elks, Jessa Rose Li, Alexander Zajack, Lance Horner, Alan Ikeda, Charles St Hill, Michael Scheidler, Shirong Chang

School of Medicine Faculty Research

Pancreatic neuroendocrine tumors (pNETs) are rare pancreatic neoplasms and are even more uncommon in the pediatric patients, thus leading to a lack of clinical research on diagnosis and management in this population. The purpose of this report is to review relevant literature and discuss a rare occurrence of a non-functioning pNET in a pediatric patient. This is a report of an 11-year-old male who presented with symptomatic anemia and was found to have a 6 cm mass near the pancreatic head with erosion into the duodenum. Surgical biopsy demonstrated a non-functioning pNET. He was successfully managed with complete surgical resection …


Mountain West States Most Dependent On The Federal Government, 2022, Saha Salahi, Caitlin J. Saladino, William E. Brown Jr. Nov 2022

Mountain West States Most Dependent On The Federal Government, 2022, Saha Salahi, Caitlin J. Saladino, William E. Brown Jr.

Economic Development & Workforce

This fact sheet synthesizes data from the SmartAsset report, “States Most Dependent on the Federal Government – 2022 Edition.” This fact sheet explores federal government dependency for the following Mountain West states: Arizona, Colorado, Nevada, New Mexico, and Utah.


Deep Learning‑Based Framework For Slide‑Based Histopathological Image Analysis, Sai Kosaraju, Jeongyeon Park, Hyun Lee, Jung Wook Yang, Mingon Kang Nov 2022

Deep Learning‑Based Framework For Slide‑Based Histopathological Image Analysis, Sai Kosaraju, Jeongyeon Park, Hyun Lee, Jung Wook Yang, Mingon Kang

Computer Science Faculty Research

Digital pathology coupled with advanced machine learning (e.g., deep learning) has been changing the paradigm of whole-slide histopathological images (WSIs) analysis. Major applications in digital pathology using machine learning include automatic cancer classification, survival analysis, and subtyping from pathological images. While most pathological image analyses are based on patch-wise processing due to the extremely large size of histopathology images, there are several applications that predict a single clinical outcome or perform pathological diagnosis per slide (e.g., cancer classification, survival analysis). However, current slide-based analyses are task-dependent, and a general framework of slide-based analysis in WSI has been seldom investigated. We …


“Best Employers”: The Impacts Of Employee Reviews And Employer Awards On Job Seekers’ Application Intentions, Yunxuan (Carrie) Zhang, Cass Shum, Amanda Mapel Belarmino Nov 2022

“Best Employers”: The Impacts Of Employee Reviews And Employer Awards On Job Seekers’ Application Intentions, Yunxuan (Carrie) Zhang, Cass Shum, Amanda Mapel Belarmino

Hospitality Faculty Research

While hospitality researchers have examined the impacts of user-generated content on customers, research regarding the impacts of employee reviews on job seekers’ application intentions is scarce. Yet, labor shortages in the hospitality industry have been amplified in recent years. The tight job market requires organizations to use aggressive and proactive recruitment strategies. As online employee reviews can attract both active and passive job seekers, organizations are increasingly advertising their jobs on these sites. This study draws on the elaboration likelihood model (ELM) and tests the boundary condition of work experience on the effects of overall star-ratings and employer awards on …


Focus On Top Tier 2.0: Student Achievement, University Of Nevada, Las Vegas Nov 2022

Focus On Top Tier 2.0: Student Achievement, University Of Nevada, Las Vegas

Focus on Top Tier 2.0

Student Achievement involves undergraduate, graduate, and professional student success as we focus on outcomes and opportunities for students at all levels. Our efforts are aligned with workforce development, which is referred to as Career Development and Progression. The work we do is vital as we educate students that are prepared for success in their personal, civic, and professional lives.


The Assembly Of Mammalian Swi/Snfchromatin Remodeling Complexes Is Regulated By Lysine-Methylation Dependent Proteolysis, Pengfei Guo, Nam Hoang, Joseph Sanchez, Elaine H. Zhang, Keshari Rajawasam, Kristiana Trinidad, Hong Sun, Hui Zhang Nov 2022

The Assembly Of Mammalian Swi/Snfchromatin Remodeling Complexes Is Regulated By Lysine-Methylation Dependent Proteolysis, Pengfei Guo, Nam Hoang, Joseph Sanchez, Elaine H. Zhang, Keshari Rajawasam, Kristiana Trinidad, Hong Sun, Hui Zhang

Chemistry and Biochemistry Faculty Research

The assembly of mammalian SWI/SNF chromatin remodeling complexes is developmentally programed, and loss/mutations of SWI/SNF subunits alter the levels of other components through proteolysis, causing cancers. Here, we show that mouse Lsd1/Kdm1a deletion causes dramatic dissolution of SWI/SNF complexes and that LSD1 demethylates the methylated lysine residues in SMARCC1 and SMARCC2 to preserve the structural integrity of SWI/SNF complexes. The methylated SMARCC1/SMARCC2 are targeted for proteolysis by L3MBTL3 and the CRL4DCAF5 ubiquitin ligase complex. We identify SMARCC1 as the critical target of LSD1 and L3MBTL3 to maintain the pluripotency and self-renewal of embryonic stem cells. L3MBTL3 also regulates SMARCC1/SMARCC2 proteolysis …