Digital Commons Network^™

Beyond Local Pathology: Exploring The Role Of Gut Microbiota In Chronic Low Back Pain Using An Integrated Behavioral And Microbiological Approach, Adan L. Redwine

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Chronic low back pain (LBP) affects millions worldwide, often originating from intervertebral disc (IVD) degeneration. While the gut microbiome modulates musculoskeletal pain, prior studies of the gut-disc axis have focused entirely on structural changes. This leaves a critical gap: does the microbiome influence the experience of discogenic pain? To answer this, an integrated approach using longitudinal gut microbiome profiling, machine learning-based behavioral phenotyping, and structural disc assessments in a rat model of discogenic LBP was utilized.

Female Sprague Dawley rats underwent one of four conditions: sham surgery, IVD injury, antibiotic-mediated microbiome depletion + IVD injury, or IVD injury + treatment …

Program Factors Influencing Investment In Nhri Leadership Mentoring Relationships, Elizabeth A. Kenes

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

The purpose of this study was to examine how specific programmatic factors influence levels of investment within mentoring relationships in a collegiate leadership mentoring program. Previous research has demonstrated the benefits of mentoring; however, much of this work has relied on self-reported data and has not directly linked program factors to clearly defined mentoring outcomes. This study addresses this gap by examining how relationship duration, meeting consistency, and training and learning predict mentoring investment using observational program data.

Using the Investment Triangle framework and an integrative theoretical framework as guiding foundations, this study examined mentoring investment within NHRI Leadership Mentoring …

Lipid Deposition In Beef Cattle, Kyra L. Elliott

Department of Animal Science: Dissertations, Theses, and Student Research

Both the amount and types of lipid deposition in beef cattle are key determinants of carcass value and meat quality. Amounts of intramuscular, subcutaneous, and visceral fat influence carcass grading outcomes, palatability, and composition traits that are important to beef producers as well as consumers. The objectives of this thesis were to: 1) compare beef grading camera estimates of intramuscular fat to amounts of chemically extractable lipid, 2) characterize the lipidomes of beef intramuscular fat, subcutaneous fat, and lean muscle using shotgun lipidomics, and 3) identify and characterize a novel genetic variant in cattle that may have important implications for …

Characterization Of Activity Changes In Gilts During Estrus Using The NuTrack Livestock Monitoring System, Emily P. Aquino

Department of Animal Science: Dissertations, Theses, and Student Research

Early and accurate detection of estrus in replacement gilts is essential for reproductive success and to ensure efficient production. The objective of this study was to evaluate activity changes occurring relative to first estrus of gilts and subsequent cycles. At ~ 110 days of age, 112 gilts were randomly assigned to 14 pens and placed under continuous video monitoring until the end of the trial at ~ 200 days of age. Daily boar exposure (~ 10 minutes per day) began at ~ 122 days of age. During this period, recorded observations included vulva scores (based on swelling and redness), mounting …

Evaluation Of Maternal Bovine Appeasing Substance Administration And Feed Access Time On Finishing Cattle Performance, Behavior, And Carcass Characteristics, Carsten L. Loseke

Department of Animal Science: Dissertations, Theses, and Student Research

Novel products and bunk management techniques may significantly influence economically relevant performance metrics of feedlot cattle. The objective of the first experiment was to evaluate maternal Bovine Appeasing Substance (mBAS) administration on performance, carcass, health, and cortisol outcomes of yearling steers. Steers (n = 1,754, initial BW = 423 kg, SD = 25 kg) were received and assigned to pens for treatment allocation. Steers received either 10 mL of the mBAS or a mineral oil placebo at initial processing and reimplant. Hair samples were collected from a subset (n = 4 steers/pen) on d 0 and d 14. …

Integrating Growth Performance And Pathogen Detection To Investigate Disease Resilience In Commercial Swine, Emmalise Hope Marie Meyer

Department of Animal Science: Dissertations, Theses, and Student Research

Disease resilience is an important trait in commercial swine production, influencing both animal health and growth performance under natural disease challenge. The primary objective of this study was to integrate growth performance data with longitudinal pathogen detection to better understand factors contributing to disease resilience in commercial pigs. A population of 1,014 pigs across four batches was monitored from weaning through the grow-finish phase, with body weights collected to calculate average daily gain (ADG). Genome-wide association analyses were conducted using BayesC and BayesIM approaches to identify genomic regions associated with growth performance. Longitudinal pathogen surveillance was performed using pen-level oral …

Tail Biting In The Nursery: Evaluating Postural, Spatial, And Behavioral Differences Between Tail Biter And Victim Pigs, Storey L. Forster

Department of Animal Science: Dissertations, Theses, and Student Research

This study examined behavioral differences among pigs classified as tail biters, victims, or neutral during the nursery period. A total of 192 undocked nursery pigs were assigned to eight pens and monitored for 30 days using the NUtrack system. Tail biting events were identified from video observations, and pigs contributing to the top 25% of observed tail biting activity were classified as tail biters. Based on weekly tail scoring, pigs assigned a maximum tail injury score ≥ 2 were classified as victims. All other pigs were considered neutral. A total of 700 tail biting events were observed. Thirty-eight pigs …

Application Of High-Pressure Processing To Improve The Color Of Dark Beef, Kara Ann Reynolds

Department of Animal Science: Dissertations, Theses, and Student Research

Deviation of beef from the bright cherry-red color preferred by consumers is often discounted or discarded at retail, resulting in substantial annual economic loss to the beef industry. One such deviation is dark cutting beef, which is characterized by a darker lean appearance that is undesirable to consumers and often rejected. Similarly, beef from mature cattle tends to exhibit a darker surface color, which can also reduce consumer acceptability. High-Pressure processing (HPP) has been investigated as a strategy to improve the color of dark cutting beef. Previous studies have focused on identifying pressure levels and holding times that optimize color …

Preclinical Animal Modeling To Investigate Redox-Mediated Molecular Dysfunction In Traumatic Brain Injury, Brandon Z. Mcdonald

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Impacts associated with traumatic brain injury (TBI) induce a multitude of neurological morbidities mediated by a spread of biochemical dysfunction. Redox stress is a significant contributor to TBI pathophysiology and several redox stress markers have substantial evidence to support their role in diagnosing preclinical and clinical TBI. However, there is a critical need to verify their translational utility through correlations with behavioral, imaging, and molecular modalities. In chapter two, we examined the therapeutic efficacy of an antioxidant treatment (NPC3) using a controlled cortical impact (CCI) mouse model of TBI. NPC3 alleviated glial expression and reactivity while influencing the expression of …

The "Evil Forest" As Threshold: A Computational Eco-Feminist Study Of The Forest In Nigerian Fantasy, Cynthia O. Mbagwu

Department of English: Dissertations, Theses, and Student Research

This study examines the representation of the “evil forest” in Nnedi Okorafor’s Zahrah the Windseeker and Ukamaka Olisakwe’s Don’t Answer When They Call Your Name, arguing that the forest functions as a liminal threshold that catalyses female transformation. Combining close reading with computational tools like TF-IDF keyword extraction in Ruby and sentiment analysis using VADER, the project investigates how pre-colonial and colonial connotations of the “evil forest” are re-imagined in contemporary Nigerian fantasy fiction. By segmenting each narrative into pre-liminal, liminal, and post-liminal phases, the study tracks shifts in sentiment toward the forest and correlates them with thematic developments …

Factors Affecting The Efficacy Of Free Choice Vitamin & Mineral Supplementation Programs, Tanner K. Wansing

Department of Animal Science: Dissertations, Theses, and Student Research

While free choice vitamin and mineral supplements are widely used within the cow/calf sector, large knowledge gaps exist around the proper management of those supplements. This thesis discusses two factors affecting the efficacy of supplementation. The first interest was the stability of vitamin A when stored with a common free choice mineral supplement. This research addressed a critical data gap by generating information on vitamin A stability within the beef industry, as current data are predominantly based in the swine industry. Vitamin A was discovered to be stable until 4 months of storage regardless of storage conditions, whereas after 4 …

Cytoplasmic Shift Of Interferon Regulatory Factors Co‐Evolved With Jawed Vertebrate Innate Immunity, Vanessa M. Hubing

Honors Program: Senior Projects (Public)

The emergence of jaws in early vertebrates introduced a novel feeding apparatus and powerful oral defenses, but it also increased the risk of physical injury and pathogen exposure. Interferon regulatory factors (IRFs) play critical roles in orches-trating innate immunity and inflammation in response to invading microbes and tissue damage, with their subcellular local-ization being essential to some IRFs' function. Our results indicate that IRF members underwent independent expansion and diversification in two distinct vertebrate lineages: jawed and jawless vertebrates. The jawed vertebrate-specific factor, IRF5, has maintained conserved nuclear export sequences throughout evolution, while newly diversified IRF members in jawed verte-brates …

Assessing Trauma Competence Among Title Ix Practitioners: Analyzing Self-Perceived Capability And Associated Factors, Aliena Hansen

Honors Program: Senior Projects (Public)

Sexual assault remains a persistent issue on college and university campuses, and the way institutions respond to survivors can significantly influence their well-being and willingness to seek support. Title IX Practitioners are often responsible for responding to reports of sexual misconduct, making trauma-informed approaches essential in their work. However, relatively little research has examined how practitioners perceive their own trauma-related capabilities. The present study examined self-perceived trauma competence among Title IX Practitioners and explored factors associated with differences in perceived competence. Participants completed a survey assessing their confidence in applying trauma-informed principles when working with complainants. Results indicated variability in …

Transforming Theory Into Action: Feminist Frameworks In Reproductive Justice Organizing, Ella R. Mitchell

Honors Program: Senior Projects (Public)

This honors thesis examines how feminist theoretical frameworks can be translated into practical strategies for reproductive justice organizing. Grounded in a reproductive justice framework and drawing on three key feminist frameworks, intersectional feminism, queer feminist theory, and feminist ethics of care, this paper explores how each provides critical tools for understanding and responding to reproductive oppression. Intersectional feminism illuminates how overlapping systems of inequality structure reproductive experiences and political marginalization. Queer feminist theory challenges heteronormative assumptions about family, gender, and reproduction, expanding reproductive justice discourse to include LGBTQ+ communities and diverse forms of kinship. Feminist ethics of care emphasizes relational …

Piano Composition And The Role Of Women In Nineteenth Century Europe, Khang Nguyen

Honors Program: Senior Projects (Public)

In the nineteenth century, women underwent a social transition, slowly obtaining more employment and educational opportunities. The lives of women piano composers were similar, with their status as women affecting their compositional careers. This thesis explores the connection between piano composition and the role of women in the nineteenth century, with a focus on Western Europe. A brief history of Europe in the nineteenth century will be given, followed by an overview on how music and the music industry were gendered at the time. Then, the lives of Clara Schumann, Fanny Hensel, and Agathe Backer Grøndahl will be analyzed as …

Lewis Carroll’S Curious Creatures: Examining Carroll’S Use Of Animal Characters Within The Context Of His Life And Victorian Era England, Nima M. Faunce

Honors Program: Senior Projects (Public)

Lewis Carroll’s Alice’s Adventures in Wonderland (1865) has maintained a wide readership since its publication, but many aspects of Carroll’s original intentions and inspirations are lost on modern readers. By examining his animal characters through the lens of his personal life and Victorian England, a deeper understanding of his novel is uncovered. Some of his characters directly reflect individuals from his real life, such as the Liddell family, while other characters provide a window into Victorian beliefs and practices. This paper investigates each of Carroll’s animal characters in order of appearance and determines possible points of inspiration from his personal …

The Consumptive Female Ideal: The Case Study Of “Snow White”, Nayla G. Torres Ruiz

Honors Program: Senior Projects (Public)

Tuberculosis (TB), also known as “consumption” or “phthisis,” was one of the most pervasive and culturally significant diseases of the eighteenth and nineteenth centuries. Despite its high mortality rate—claiming more lives than cholera and smallpox combined—TB was widely romanticized in art, literature, and popular culture, particularly as a feminine disease associated with beauty, purity, fragility, and moral virtue. The physical traits of consumption—pale skin, rosy cheeks, red lips, glassy eyes, and a slender, delicate frame—became an ideal of female beauty, one that many women sought to emulate through restrictive diets, toxic cosmetics, and evolving fashion trends, such as increasingly tight …

Hidden Word Treasure Hunt: Design Of Dyslexia Game-Based Intervention, Olivia N. Epp

Honors Program: Senior Projects (Public)

For this creative project, a literacy intervention board game entitled Hidden Word Treasure Hunt was designed for elementary students with dyslexia. Developmental Dyslexia is a neurodevelopmental learning disability which causes reading and writing difficulties. The goal of the intervention is to provide a fun and engaging way for students with dyslexia to practice skills like spelling, phonemic awareness, rhyming, and more. The game includes multiple choice question cards (treasure cards) and challenge spelling cards (key cards), and the student also has the opportunity to practice spelling the words with a whiteboard and marker. The student and SLP can play their …

Ferrari N.V. Capstone Strategic Report, Isabel Pillaii

Honors Program: Senior Projects (Public)

This report explores the strategic position of Ferrari through Porter’s Five Forces, PESTEL, VRIO Analysis, Value Chain, and SWOT analysis. Then the report offers recommendations to the main tensions and strategic dilemmas before finally making remarks on the risks and limitations of this report. This report remarks on the strong strategic position of Ferrari due to branding, and its strong performance in Formula 1 racing and overall product specifications. It also remarks on Ferrari’s exposure to political, economic, and legal risk related to sustainability, supply chain stability, and exchange rate risk. This report recommends that Ferrari continues investing in electric …

Killing The Hot Vampire: Gender, Camp, And The Stakes Of Desire In Nineteenth-Century Vampire Fiction, Ella G. Kehr

Department of English: Dissertations, Theses, and Student Research

This thesis examines the representation and destruction of the Hot Vampire in nineteenth-century vampire fiction, focusing on Dracula (1897), Carmilla (1872), and The Blood of the Vampire (1897). It argues that these texts construct the female vampire as a figure of excessive sexuality, independence, and deviance which causes both societal (patriarchal) and narrative disruption. In the male-authored texts, the construction of the Hot Vampire is exaggerated beyond the neat bounds of gothic excess and monster theory. The destruction of these vampires also follows this unbound extravagance. The Hot Vampire’s death becomes the ritualized restoration of patriarchal order, which is often …

Exploring Determinants Of Value For Money And Bundling Effects On Public–Private Partnership Projects: A Data-Driven Empirical Study, Gaurav Khadka

Department of Construction Engineering and Management: Dissertations, Theses, and Student Research

Public–private partnerships (P3s) are widely used to deliver public infrastructure, yet current understanding of what drives variation in Value for Money (VfM) and the effectiveness of project delivery strategies such as bundling remains largely conceptual and grounded in perception-based evidence. Consequently, governments lack empirically validated guidance on which project, financial, and macroeconomic conditions meaningfully influence VfM outcomes and whether bundling improves project performance within the P3 context. This study addresses these gaps through an integrated analysis of 180 Canadian P3 infrastructure projects (1992–2020), one of the most comprehensive datasets of realized VfM internationally, incorporating project characteristics, financial arrangements, and macroeconomic …

Population Structure And Connectivity Of Atlantic Nurse Sharks (Ginglymostoma Cirratum) In The Bahamas And Outlying Areas, Baylie A. Fadool

School of Natural Resources: Dissertations, Theses, and Student Research

Understanding animal ecosystem dynamics and behavior at the individual and population levels is essential for identifying life-history patterns and important areas for management and conservation. Marine fish species often face minimal geographic barriers to movement, but environmental (temperature, salinity, current) or intrinsic barriers (philopatry, site fidelity) can dictate gene flow. Atlantic nurse sharks (Ginglymostoma cirratum) are an International Union for Conservation of Nature (IUCN) listed ‘vulnerable’ species and present a unique opportunity to investigate patterns of population structure and connectivity in large-bodied sharks. They are benthic and display reproductive philopatry, with recent work suggesting they have the capacity …

Exploring Student Sensemaking During A Collaborative Biology Lab, Rebecca Kollipara

School of Natural Resources: Dissertations, Theses, and Student Research

Collaborative learning environments offer valuable opportunities for students to construct scientific understanding through dialogue, yet the conditions that support sustained conceptual reasoning in group settings remain incompletely understood. While prior research has identified discourse moves associated with productive collaboration, less attention has been paid to how those moves function together across turns and conceptual boundaries, or how individual student characteristics shape their emergence. This thesis examines collaborative sensemaking in an undergraduate General Biology II course, drawing on two complementary analyses of student discourse during a bryophyte life-cycle modeling activity.

Chapter 1 investigates how student groups participated in sensemaking and how …

Examining Extremes: A Comparison Between Machine Learning Based Southwestern Conus Seasonal Drought And High Fire Risk Event Synoptic Climatologies, Ethan M. Greenberg

School of Natural Resources: Dissertations, Theses, and Student Research

The Southwestern United States (SW CONUS), comprised of California, Arizona, Nevada, and Utah, is a vast region that tens of millions of people call home. Hosting ecosystems ranging from grasslands and shrublands to temperate forests and climate zones ranging from Mediterranean climates to arid deserts, the region is nearly unanimously prone to intense droughts and devastating wildfires. While fire weather conditions and drought are often studied separately or mentioned as background conditions for the other, there are relatively few studies that compare the typical meteorological conditions between the two. This study aims to more closely understand the meteorological relationship between …

Gaining Mussel: Evaluating Reintroduction Success Of Native Freshwater Mussels In Nebraska And Eastern Wyoming, Travis A. Moore

School of Natural Resources: Dissertations, Theses, and Student Research

Freshwater mussels are one of the most imperiled groups of organisms in North America, with over two-thirds of species considered threatened, endangered, or of special concern. These long-lived bivalves are essential to stream ecosystems, acting as filter feeders that improve water quality and as indicators of aquatic health. In Nebraska and Wyoming, native mussel populations have declined sharply due to habitat modification, pollution, and reduced connectivity. Two species of particular concern are the Plain Pocketbook Lampsilis cardium, listed as Tier I at-risk in Nebraska, and the Fatmucket Lampsilis siliquoidea, a Tier II at-risk species. In response, state agencies …

Honeybee Colony Response To Probiotic Supplementation And Grazing Practices, Katya M. Busenitz

Department of Entomology: Dissertations, Theses, and Student Research

Honeybee health is a complicated issue. Studies were conducted to scale honeybee (Apis mellifera) health from the microbiome to the landscape. Colony health parameters were measured in response to probiotic administration. Colony capped brood production, colony summer weight gain, and Varroa mite incidence were measured. I found that applications of over-the-counter probiotics did not improve the measured parameters of honeybee health compared to a control group. Results suggest that probiotic application was not beneficial but positive benefits may depend on application method, brand, or strain of probiotics used. I also examined how colony health metrics responded to different …

Charting The Course: The Influence Of Mentorship On Women's Careers In Outdoor Education, Jessica M. Callahan

Department of Teaching, Learning, and Teacher Education: Theses and Other Student Research

Mentorship is a significant contributor to women’s experiences working in outdoor education, yet it remains underresearched in a formal capacity. This study aims to answer the central research question of “What meanings do women give to mentorship experiences in relation to their career development, retention, and professional identity?” A qualitative, phenomenological approach was chosen to be most appropriate, and both synchronous interviews and an online survey were used to collect data. In total, data was collected from 24 participants, which was analyzed using thematic analysis. Identified themes were categorized by the topics of mentor qualities and impact on self, mentorship …

Spatiotemporal Variability Of Differential Reflectivity (Zdr) Columns Due To Environment In Tornadic And Nontornadic Supercells, Adrianne J. Engel

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Polarimetric signatures have been of interest to researchers and operational forecasters since the completion of the WSR-88D network upgrade in 2013. Differential reflectivity (Z_DR) columns, which are collocated with supercell updrafts, are the focus of this study. Microphysical distributions within these signatures can be used to infer updraft strength and elucidate the processes responsible for updraft intensity and evolution. Z_DR column depth, areal extent, and variability of these metrics through space and time are assessed in a dataset of 114 nontornadic and tornadic supercells. Means of column metrics in nontornadic, non-tornado-producing, and tornado-producing analysis times are evaluated …

Undergraduate Design Spine For Biological Systems Engineering And Agricultural Engineering, David Mabie, Derek M. Heeren, Deepak R. Keshwani, Heidi A. Diefes-Dux, Nicole M. Iverson, Rachel Padrnos, Greg Bashford, Taryn King, Jenna Hefley, Curtis L. Tomasevicz

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Engineering education is undergoing a shift toward preparing graduates with demonstrated capabilities that integrate technical knowledge, durable skills, and the ability to adapt in a rapidly evolving professional landscape. This paper describes a comprehensive redesign of the undergraduate curriculum in Biological Systems Engineering (BSEN) and Agricultural Engineering (AGEN) at the University of Nebraska–Lincoln. The redesign is grounded in a “Design Spine” framework, which distributes and scaffolds design experiences across all four years of the program to support progressive development of problem solving, systems thinking, communication, and professional skills. The curriculum is organized around clearly defined capabilities and threads that connect …

Assessment Of Super Absorbent Polymer In Soybean Cultivation Under Variable Irrigation Conditions In Nebraska, Ankit Chandra, Abia Katimbo, Ronaldo Marchezan, Martha Nabaggala

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Water limitation is a primary constraint to soybean productivity in both irrigated and rainfed systems. Soil-applied superabsorbent polymers (SAPs) have been proposed as a strategy to buffer crops against episodic drought stress; however, their physiological and agronomic performance under varying irrigation regimes remains insufficiently quantified. Here, we evaluate three biodegradable polymer formulations: broader granules (BB), original granules (OG), and powder (PWR) applied at low (17.7 lb/ac) and high (120 lb/ac) rates under full (100% ET), reduced (50% ET), and no irrigation conditions in western Nebraska. Polymer application had negligible effects under full irrigation, with yields comparable to untreated controls. Under …