Digital Commons Network^™

Characterizing Feedlot Feed Using Depth Cameras And Imaging Technology, Jean Niwenshuti

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Imaging technology is a growing field that provides solutions in many areas from manufacturing to agriculture. Through previous research, imaging technologies have been studied in livestock farming to monitor the animal’s health and welfare in the production process. However, the feedlot industry is still behind in validating the feasibility to use some of these technologies nor to adopt such technologies to address challenges the industry is facing, such as lack of skilled labor.This work proposes using novel imaging methods to identify feed types and estimate the amount of feed remaining in a typical Midwestern feedlot feed bunk. These methods have …

Potential Of Mirnas In Plasma Extracellular Vesicle For The Stratification Of Prostate Cancer In A South African Population, Dada Oluwaseyi Temilola, Martha Wium, Juliano Paccez, Azola Samkele Salukazana, Hasan H. Otu, Giuseppina M. Carbone, Lisa Kaestner, Stefano Cacciatore, Luiz Fernando Zerbini

Department of Electrical and Computer Engineering: Faculty Publications

Prostate cancer (PCa) is the most common cause of cancer death among African men. The analysis of microRNAs (miRNAs) in plasma extracellular vesicles (EVs) can be utilized as a non-invasive tool for the diagnosis of PCa. In this study, we used small RNA sequencing to profile miRNAs cargo in plasma EVs from South African PCa patients. We evaluated the differential expression of miRNAs between low and high Gleason scores in the plasma EVs of South African patients and in the prostatic tissue from data available in the Cancer Genome Atlas (TCGA) Data Portal. We identified 7 miRNAs differently expressed in …

Flex-Ro: Development And Testing Of A Semi-Autonomous Robotic Planter, Ian Tempelmeyer

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The need for more crop production with minimal negative environmental impact is a continuing pursuit around the world. As the population continues to increase at an exponential rate, the demand for food, fiber, fuel, and energy also increases. Robotics and automation are seen as one of the useful technology tools to increase field productivity. This work specifically focuses on addressing labor challenges for one of the most important field operations, planting. A robotic planter unit was designed as part of this work that mounted on unmanned ground vehicle (UGV) to perform autonomous planting operation in a 1 ha (2.5 acre) …

Auricularia Auricula Polysaccharides Attenuate Obesity In Mice Through Gut Commensal Papillibacter Cinnamivorans, Xin Zong, Hao Zhang, Luoyi Zhu, Edward C. Deehan, Jie Fu, Yizhen Wang, Mingliang Jin

Department of Nutrition and Health Sciences: Faculty Publications

Introduction: Auricularia auricula is a well-known traditional edible and medical fungus with high nutritional and pharmacological values, as well as metabolic and immunoregulatory properties. Nondigestible fermentable polysaccharides are identified as primary bioactive constituents of Auricularia auricula extracts. However, the exact mechanisms underlying the effects of Auricularia auricula polysaccharides (AAP) on obesity and related metabolic endpoints, including the role of the gut microbiota, remain insufficiently understood.

Methods: The effects of AAP on obesity were assessed within high-fat diet (HFD)-based mice through obesity trait analysis and metabolomic profiling. To determine the mechanistic role of the gut microbiota in observed anti-obesogenic …

Integration Of Meta-Multi-Omics Data Using Probabilistic Graphs And External Knowledge, Handan Can, Sree K. Chanumolu, Barbara D. D., Sophie Alvarez, Michael J. Naldrett, Gülhan Ünlü, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

Multi-omics has the promise to provide a detailed molecular picture of biological systems. Although obtaining multi-omics data is relatively easy, methods that analyze such data have been lagging. In this paper, we present an algorithm that uses probabilistic graph representations and external knowledge to perform optimal structure learning and deduce a multifarious interaction network for multi-omics data from a bacterial community. Kefir grain, a microbial community that ferments milk and creates kefir, represents a self-renewing, stable, natural microbial community. Kefir has been shown to have a wide range of health benefits. We obtained a controlled bacterial community using the two …

Early Career Aquatic Scientists Forge New Connections At Eco-Das Xv, Olivia J. Graham, Alia Al-Haj, Eleanor C. Arrington, Emily R. Arsenault, Carolina C. Barbosa, Kadir Bice, Evie Brahmstedt, S. River D. Bryant, Xun Cai, Stacy Calhoun-Grosch, Joshua Culpepper, Katherine Dale, Derek J. Detweiler, Katlin D. Doughty, Kyle A. Emery, Kara Gadeken, Laura Griffiths, Atefeh Hosseini, Catriona Jones, Hadis Miraly, Alexander W. Mott, Karla Münzner, Igor Ogashawara, Carly R. Olson, Joseph S. Rabaey, Walter A. Rich, Phoenix A. Rogers, Meredith Evans Seeley, Lorena Selak, Qipei Shangguan, Kelsey J. Solomon, Xinyu Sun, Spencer J. Tassone, Audrey Thellman, John Tracey, Jilian Xiong, Tianfei Xue

School of Natural Resources: Faculty Publications

A sense of kuleana (personal responsibility) in caring for the land and sea. An appreciation for laulima (many hands cooperating). An understanding of aloha ’āina (love of the land). The University of Hawai’i at Manoa hosted the 2023 Ecological Dissertations in Aquatic Sciences (Eco-DAS) program, which fostered each of these intentions by bringing together a team of early career aquatic ecologists for a week of networking and collaborative, interdisciplinary project development (Fig. 1).

The Casnr L.I.N.K.S. Newsletter: Learning Innovation Network For K-12 Schools, Volume 2, Edition 7, August 2023, Bailey Feit, Tammy Mittelstet

CASNR L.I.N.K.S. Newsletter

The purpose of this L.I.N.K.S. newsletter is to provide a monthly update of opportunities and resources for K-12 schools to connect with the University of Nebraska's CASNR Food, Energy, Water, and Societal Systems (FEWS²) programs and partners.

Included in this edition:

K-12 curriculum and resources

Nebraska Game and Parks Wildlife Education

United States Forest Service Environmental Education

Upcoming events

Experience the Power of Red Visit Day

Panhandle-REEC Career Day-Scottsbluff

Eastern Nebraska World Food Prize

Cultivando Caminos (Cultivating Pathways) Conference

NJAS State Science Fair

Career exploration - pathways for students who like technology

UNL School of Natural Resources: Agronomy and Horticulture, …

Nebline, August/September 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Understanding the “WHY” of Challenging Behavior in Young Children Can Help With Strategies by Hayley Jackson

5 Reasons to Support Your Farmers Market by Tara Dunker

Recipes of the Month: Chocolate Zucchini Muffins and Fresh Salsa by Kayla Colgrove

Time for Fall Lawn Seeding by Sarah Browning

Garden Guide: Things to Do This Month

Using Water Resources More Efficiently by Becky Schuerman

Parasitoids: Nature’s Biocontrol by Kait Chapman

Lancaster County Super Fair, Aug. 3-12, 2023

Webinars Addressing Biting Pests in Early Childhood and School Settings

Ag Landlord/Tenant Workshop

4-H Announcements for 4-H'ers or 4-H Volunteers

Heart of 4-H Volunteer Award: …

Investigation And Modification Of Single-Post Aluminum Sign Supports For Mash Crashworthiness, Rodrigo Quintero

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

Traffic signs are used to display information to drivers and are one of the most commonly encountered roadside structures. Although they are a vital traffic control device, signs can pose a significant safety risk to errant drivers due to their extensive placement and proximity to the roadway. Therefore, traffic signs and their supports must be designed with crashworthiness in mind to reduce the likelihood of injury in the event of a vehicle impact. Prior to 2009, these systems were evaluated using the crash testing criteria found in the National Cooperative Highway Research Program (NCHRP) Report No. 350 [1]. Currently, they …

Experimental Study Of Linux Flightsize Estimation, Mingrui Zhang

School of Computing: Dissertations, Theses, and Student Research

Transmission Control Protocol (TCP) is a fundamental Internet protocol responsible for controlling and coordinating the Internet traffic. As a result, TCP significantly influences the overall performance and stability of the Internet. One critical information required by a TCP connection to make decisions is FlightSize, which is the total amount of outstanding data contributed by the connection to the Internet. The FlightSize information is used by a TCP connection to determine its future sending rate and also avoid traffic congestion and collapse in the Internet. Consequently, an inaccurate estimation of FlightSize can result in degraded performance and instability of the Internet. …

Seek And Classify: End-To-End Joint Multi-Signal Detection And Classification Using Deep Learning, Prashant Subedi

School of Computing: Dissertations, Theses, and Student Research

The rise in the use of wireless communication has led to the problem of spectrum scarcity in licensed bands. The popularity of Internet of Things (IoT) requires innovative solutions that maximize the use of available spectrum to support the increasing number of connected devices. This thesis tackles two significant problems in wireless communication: the need for efficient spectrum sensing techniques and the scarcity of large, diverse raw in-phase (I) and quadrature (Q) datasets.

The ability to detect and classify modulation of the signals efficiently can enable a cognitive radio to monitor the spectrum activity in real time and utilize unused …

Energy Dissipation Optimization For Circular Culverts, Chi Zhang

Department of Civil and Environmental Engineering: Dissertations, Theses, and Student Research

The principal goal of the current research is to develop and improve weir-based energy dissipator designs at the outlets of circular culverts. In the present case, we are interested in full-length weirs and staggered weirs. A review of related literature was completed and reported; a test facility was carefully designed and constructed to collect flow information in a basin downstream of a model broken-back circular culvert; instrumentation was installed to measure discharges, streamwise depths, and velocities. Both full-length and staggered weirs were tested over a range of discharges and tailwaters. The two types of weirs were subjected to two types …

Wild Bee Utilization Of Engineered Pollinator Habitat In Eastern Nebraska Agroecosystems, Shianne Lindsay

Department of Entomology: Dissertations, Theses, and Student Research

Habitat loss is a leading cause of biodiversity decline globally. Further, loss of natural and semi-natural habitat is a major factor contributing to declines in wild pollinator populations. Among intensive agroecosystems in the Midwestern United States, corn, soy, and small grain fields dominate the landscape and leave limited space available for forb-rich habitat that supports diverse pollinator assemblages to exist and thrive. There are numerous barriers and challenges to integrating pollinator habitat into such agroecosystems and quantitative assessments of impacts on pollinator communities are largely lacking. The establishment of high-quality pollinator habitat can provide both high flower abundance and species …

Prairie Protector: Student Development Of Systems Thinking Habits In The Context Of Agroecosystems, Erin Ingram, Jennifer Keshwani

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Introduction: The Great Plains ecosystem offers an opportunity for young people to gain knowledge about intricate systems through practical learning. The spread of woody plants into grasslands, known as the “Green Glacier,” poses a risk to biodiversity and animal production. Although effective management strategies exist, some land managers are reluctant to use them. It is critical to cultivate a scientifically literate population that can think systematically and make informed decisions based on STEM principles to address such complex agroecosystem problems.

Methods: In this study, semi-structured focus group discussions with high school students were analyzed to determine whether Prairie …

Utilizing Mitotalens To Investigate Large Repeats In The Arabidopsis Thaliana Mitochondrial Genome, Cambelle Johnson

Honors Program: Senior Projects (Public)

Mitochondria are a silly place to store the genetic code. In spite of the ever-present threat of oxidative DNA damage, plant mitochondrial genomes are expansive in size and include noncoding repeat sequences, a small number of which are greater than 1kilobase (kb), known hereafter as “large repeats” (LR). This theme of one or more large repeat pairs is shared across most flowering plants, lending to the belief that they somehow contribute to the plant’s fitness. These repeats exhibit high rates of rearrangement and recombination. Studying repeats in vivo could have large scale implications in hybrid crop production enhancement including eliminating …

Growth And Emergent Functionalities Of Oxide Thin Films Utilizing Interface Engineering, Detian Yang

Department of Physics and Astronomy: Dissertations, Theses, and Student Research

Complex oxide interfaces have offered intriguing novel emergent phenomena and multiple functionalities through interfacial reconstructions of spin, orbital, charge, and lattice degrees of freedom. Interface engineering via manipulating interfacial interaction, defects and multiple interfacial quantum charges and orders constitutes the essential method and technique to achieve desired functionalities in oxide heterostructures. In this thesis, shown are two examples of utilizing interfacial reconstruction and interfacial strain engineering to achieve intrinsic exchange bias and realize epitaxial growth of mixed-valence hexagonal manganite thin films, respectively.

Firstly, we demonstrated intrinsic exchange bias induced by interfacial reconstruction in Ni_xCo_yFe_3-x-yO …

Assessment Of Integrated Nutrient Management Practices On Soil Health And Nitrogen Efficiency In Cropping Systems, María José Oviedo Ventura

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Groundwater quality in Nebraska is threatened by non-point source contributions of nitrate-nitrogen, primarily by agricultural systems. Intensive crop and livestock production that neglects the value of manure in crop fertility plans can contribute to soil degradation and natural resource impairment. The purpose of this study is to evaluate opportunities to improve agricultural nutrient management throughout Nebraska by quantifying opportunities for manure distribution, evaluating soil health implications of organic and inorganic soil amendments, and assessing nutrient management practices focused on reducing nitrate leaching in agricultural soils.

This study described in Chapter 2 provides a spatial illustration of nitrogen and phosphorus balances …

Data-Driven Framework For Predicting And Scheduling Household Charging Of Evs, Ahmad Almaghrebi

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

The increasing prevalence of EV charging poses challenges for power grid stability and quality due to high charging load demands. Without effective energy management strategies for EV charging, the simultaneous power demand from numerous EVs can strain the electric grid, impacting power quality and the wholesale electricity market. To address these challenges, this dissertation presents a comprehensive framework comprising five critical tasks: analyzing EV charging behavior, optimizing charging schedules, developing predictive models, analyzing aggregated impacts, and evaluating implications of predicted user behavior on scheduling. By examining EV charging behavior at household and public charging stations, this study aims to understand …

Framework Development For Evaluating The Impacts Of Nursing Home Acoustics On Older Adults, Brian J. Puckett

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

Nursing homes are complex, multi-faceted environments which provide necessary medical supervision and housing for older adults in need of long-term care. With cognitive and sensory impairments being common adversities faced throughout the aging process, research is needed to better understand characteristics of the built environment which may contribute to difficulties for nursing home residents. Noise, in particular, has been shown to be problematic for nursing home residents in a variety of ways including disrupting sleep, associating with behavioral and psychological symptoms of dementia, and leading to increased readmission to hospitals due to verbal communication misunderstandings. As the older adult population …

Influence Of Overcooking On Food Digestibility And In Vitro Fermentation, Wensheng Ding

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Areas of char or overcooking commonly appear in foods people consume. It has been reported that overcooked food is harmful to human health. However, little research exists on the effect of overcooking on in vitro protein and starch digestibility and gut microbial fermentation. This study aimed to reveal the connection between overcooking and in vitro protein and starch digestibility and gut microbial fermentation. In vitro protein digestibility of an overcooked ground beef patty was almost half that of a standard cooked sample (27 ± 2% versus 48 ± 6%, respectively; p = 0.02). Whole wheat bread protein digestibility was also …

Drinking Water Quality Management Progress In Ontario, Two Decades After Walkerton, Megan Fuller, Emma Wells, Laith Furatian, Ian Douglas, Kaycie Lane

Department of Civil and Environmental Engineering: Faculty Publications

Following the waterborne disease outbreak in Walkerton, Ontario, the province made significant efforts to implement recommendations of the public inquiry that resulted. As Ontario reformed its drinking water sector, other jurisdictions were advancing risk-based quality management frameworks for drinking water, including the World Health Organization (WHO) through its water safety plan (WSP) framework. Two decades after the Walkerton tragedy, this paper seeks to: (i) evaluate alignment of Ontario’s Drinking Water Quality Management Standard (DWQMS) with the WSP framework (ii) review readily available data for evidence that Ontario’s DWQMS implementation has improved drinking water safety and promoted a preventive approach through …

Lunchbox Hydroponics, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

Growing plants with hydroponics is less complicated than it may appear when the fundamentals of the system are understood. This publication provides instruction on the construction of a simple hydroponic prototype suitable for school or youth education programs. The fundamentals of hydroponics, basic design of a solution system, seed starting, creating a nutrient solution, and plant care are explored through this engaging project. This prototype can successfully be used to grow small edible and herb plants in the classroom or at home.

A Portable, Low Power Radiation Detection And Identification System For High Count Rate, Long Term Monitoring, Samuel J. Murray

Department of Electrical and Computer Engineering: Dissertations, Theses, and Student Research

This dissertation presents the design of a novel radiation detection and identification system that can operate continuously over a period of 8 days while detecting at 30,000 counts per second, consuming a total of 11 mW. The entire system is highly integrated, containing a gamma ray detector, a high voltage detector power supply, and a multichannel analyzer (MCA) system-on-a-chip (SoC), which are all combined into a compact form using a multi-level, configurable printed circuit board design. The MCA SoC, fabricated using a 65 nm CMOS technology, features two enabling resources to allow low power detections at high count rates for …

Leveraging Eco-Evolutionary Models For Gene Drive Risk Assessment, Matthew A. Combs, Andrew J. Golnar, Justin M. Overcash, Alun L. Lloyd, Keith R. Hayes, David A. O'Brochta, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

Engineered gene drives create potential for both widespread benefits and irreversible harms to ecosystems. CRISPR-based systems of allelic conversion have rapidly accelerated gene drive research across diverse taxa, putting field trials and their necessary risk assessments on the horizon. Dynamic processbased models provide flexible quantitative platforms to predict gene drive outcomes in the context of system-specific ecological and evolutionary features. Here, we synthesize gene drive dynamic modeling studies to highlight research trends, knowledge gaps, and emergent principles, organized around their genetic, demographic, spatial, environmental, and implementation features. We identify the phenomena that most significantly influence model predictions, discuss limitations of …

Transcriptional Regulation Of The Synthesis And Secretion Of Farnesol In The Fungus Candida Albicans: Examination Of The Homann Transcription Regulator Knockout Collection, Daniel Gutzmann, Jaxon J. Kramer, Brigid M. Toomey, Cory Honsinger Thomas Boone, Audrey L. Atkin, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Candida albicans is an efficient colonizer of human gastrointestinal tracts and skin and is an opportunistic pathogen. C. albicans exhibits morphological plasticity, and the ability to switch between yeast and filamentous morphologies is associated with virulence. One regulator of this switch is the quorum sensing molecule farnesol that is produced by C. albicans throughout growth. However, the synthesis, secretion, regulation, and turnover of farnesol are not fully understood. To address this, we used our improved farnesol assay to screen a transcription regulator knockout library for differences in farnesol accumulation in whole cultures, pellets, and supernatants. All screened mutants produced …

Transcriptional Regulation Of The Synthesis And Secretion Of Farnesol In The Fungus Candida Albicans: Examination Of The Homann Transcription Regulator Knockout Collection, Daniel Gutzmann, Jaxon J. Kramer, Brigid M. Toomey, Cory Honsinger Thomas Boone, Audrey L. Atkin, Kenneth Nickerson

School of Biological Sciences: Faculty Publications

Candida albicans is an efficient colonizer of human gastrointestinal tracts and skin and is an opportunistic pathogen. C. albicans exhibits morphological plasticity, and the ability to switch between yeast and filamentous morphologies is associated with virulence. One regulator of this switch is the quorum sensing molecule farnesol that is produced by C. albicans throughout growth. However, the synthesis, secretion, regulation, and turnover of farnesol are not fully understood. To address this, we used our improved farnesol assay to screen a transcription regulator knockout library for differences in farnesol accumulation in whole cultures, pellets, and supernatants. All screened mutants …

Critical Factors And Individual Decision Making In Earthquake And Tsunami Preparedness Among Coastal Communities In Oregon U.S.A., Shelley Olds

School of Natural Resources: Dissertations, Theses, and Student Research

Earthquakes and tsunamis are natural hazards that cause considerable loss of life, destruction of property and infrastructure, and economic damage. Preparedness is critical in regions of significant earthquake and tsunami risk. A key challenge for organizations that manage hazard preparedness and response is mobilizing earthquake preparedness among individuals and families. This dissertation provides an examination of individual earthquake and tsunami preparedness from both a global and regional perspective. It comprises two publications that explore various aspects of disaster preparedness.

Chapter One provides an overview of global seismic and tsunami hazards, along with potential impacts, and addresses the challenges in promoting …

Increasing Rye Cover Crop Biomass Production After Corn Residue Removal To Balance Economics And Soil Health, Sabrina J. Ruis, Humberto Blanco-Canqui, Paul J. Jasa, Glen Slater, Richard B. Ferguson

Department of Agronomy and Horticulture: Faculty Publications

Low or variable cover crop (CC) biomass production could limit CC benefits. Longer CC growing periods via late termination could increase CC benefits, especially under limited crop residue return. We studied whether early (2–3 wk before planting)- or late (at planting)-terminated winter rye (Secale cereale L.) CC maintains soil properties, crop yields, and farm income under 0%, 25%, 50%, 75%, and 100% corn (Zea mays L.) residue removal in rainfed and irrigated no-till in the U.S. Great Plains after 6 yr. Early-terminated CCs produced < 1 Mg ha^-1 of biomass while late-terminated CCs averaged 1.6 Mg ha^-1 at the rainfed …

Soybean Tolerance To Ultra-Low Doses Of Dicamba: Hormesis Or Not, Luka Milosevic, O. Adewale Osipitan, Jon E. Scott, Stevan Z. Knezevic

Department of Agronomy and Horticulture: Faculty Publications

The widespread use of dicamba across the soybean growing regions of the United States resulted in increased off-target movement of this herbicide to susceptible vegetations. There are speculations that a drift of sublethal (ultra-low) doses of dicamba can enhance soybean growth and yield through a phenomenon called hormesis. Field studies were conducted during 2018 and 2019 to determine whether soybean growth and yield can be enhanced with ultra-low doses (0.0112–56 g ae ha^-1) of dicamba, applied at three growth stages (V2, R1 and R2). There was no evidence that the ultra-low doses of dicamba (0.0112–56 g ae ha …

Signal Classification Based On Analog Computing Using Mems Network, Mohammad Okour

Durham School of Architectural Engineering and Construction: Dissertations, Theses, and Student Research

The rising complexity of machine learning algorithms and Artificial Intelligence in many applications, such as smart building, has prompted the development of alternate computing options. Because of their compact size, low power consumption, and diverse functionality, microelectromechanical systems (MEMS) have emerged as a possible candidate. This thesis focuses on using MEMS networks as computing units to classify a simple signal classification task using neural network methodology. The study intends to show the potential of using MEMS as an analog computing unit by discussing the advantage of the bi-stability pull-in behavior and hysteresis to create an accurate classifier of these waveforms. …