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Companion Planting: Effects Of Radishes On Squash Bugs, Daniel Dejong

Department of Environmental Studies: Undergraduate Student Theses

Throughout history, humans have continuously looked for easier and improved ways of growing our food. Over centuries of hybridizing crop varieties and more recently the invention and implementation of genetically modified (GM) seeds, we have created a food system that is dependent on chemical and seed companies to provide protection from environmental pests. The purpose of this thesis is not to discredit or dissuade someone from using GM seeds, but to take a look at some methods that could protect crops from environmental pests naturally, either for organic farmers or home gardeners. Specifically this review will examine methods of controlling …

Companion Planting: Effects Of Radishes On Squash Bugs, Daniel Dejong

Department of Environmental Studies: Undergraduate Student Theses

Throughout history, humans have continuously looked for easier and improved ways of growing our food. Over centuries of hybridizing crop varieties and more recently the invention and implementation of genetically modified (GM) seeds, we have created a food system that is dependent on chemical and seed companies to provide protection from environmental pests. The purpose of this thesis is not to discredit or dissuade someone from using GM seeds, but to take a look at some methods that could protect crops from environmental pests naturally, either for organic farmers or home gardeners. Specifically this review will examine methods of controlling …

Zika Virus Encoding Nonglycosylated Envelope Protein Is Attenuated And Defective In Neuroinvasion, Arun S. Annamalai, Aryamav Pattnaik, Bikash R. Sahoo, Ezhumalai Muthukrishnan, Sathish Kumar Natarajan, David Steffen, Hiep Vu, Gustavo Delho, Fernando Osorio, Thomas M. Petro, Shi-Hua Xiang, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

Zika virus (ZIKV), a mosquito-transmitted flavivirus responsible for sporadic outbreaks of mild and febrile illness in Africa and Asia, reemerged in the last decade causing serious human diseases, including microcephaly, congenital malformations, and Guillain-Barré syndrome. Although genomic and phylogenetic analyses suggest that genetic evolution may have led to the enhanced virulence of ZIKV, experimental evidence supporting the role of specific genetic changes in virulence is currently lacking. One sequence motif, VNDT, containing an N-linked glycosylation site in the envelope (E) protein, is polymorphic; it is absent in many of the African isolates but present in all isolates from the recent …

Understanding And Finding Solutions To The Problem Of Sedimentation In The National Wildlife Refuge System, Max Post Van Der Burg, Karen E. Jenni, Timothy L. Nieman, Josh D. Eash, Gregory A. Knutsen

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The National Wildlife Refuge System (Refuge System) is a collection of public lands maintained by the U.S. Fish and Wildlife Service for migratory birds and other wildlife. Wetlands on individual National Wildlife Refuges (Refuges) may be at risk of increased sedimentation because of land use and water management practices. Increased sedimentation can reduce wetland habitat quality by altering hydrologic function, degrading water quality, and inhibiting growth of vegetation and invertebrates. On Refuges negatively affected by increased sedimentation, managers have to address complex questions about how to best remediate and mitigate the negative effects. The best way to account for these …

Notes: Range Extension Of The Virginia Opossum (Didelphis Virginiana) In North Dakota, Lisa L. Walsh, Robert Seabloom, Cody W. Thompson

The Prairie Naturalist

The Virginia opossum (Didelphis virginiana) is broadly distributed across North America from Costa Rica in the south to southern Ontario in the north and from the southern Great Plains in the west to the eastern United States. The Virginia opossum also was introduced multiple times to thePacific Coast beginning in the late 1800s and has established populations in that region. This species is a habitat generalist known to frequent wetland and hardwood habitats but also can be found in grasslands, along forest edges, and in agricultural and suburban settings throughout its range. However, the Virginia opossum is adapted …

Notes On The Tiger Beetles (Coleoptera: Carabidae: Cicindelinae) Of Vietnam. 135. Contribution Towards The Knowledge Of Cicindelinae, Jürgen Wiesner, Aligi Bandinelli, Andrey Matalin

Insecta Mundi

Distributional records of the 177 tiger beetle species and subspecies known for Vietnam are given together with a key and habitus photos (Coleoptera: Carabidae: Cicindelinae). Tricondyla (Tricondyla) macrodera abruptesculpta Horn, 1925, Protocollyris festiva Naviaux, 2008, Neocollyris (Brachycollyris) purpureomaculata borea Naviaux, 1994, Neocollyris (Brachycollyris) torosa Naviaux, 2010, Neocollyris (Leptocollyris) rogeri Shook and Wu, 2006, Neocollyris (Leptocollyris) laosensis Naviaux, 1999, Neocollyris (Stenocollyris) rubens (Bates, 1878), Neocollyris (Pachycollyris) tricolor Naviaux, 1991, Neocollyris (Pachycollyris) panfilovi Naviaux and Matalin, 2002, Therates rugulosus Horn, 1900, Therates bannapecolus Sawada and Wiesner, 1999, Calochroa interruptofasciata flavolineata (Chaudoir, 1865), Cylindera (Ifasina) cyclobregma Acciavatti and Pearson, 1989, Cylindera (Ifasina) decolorata …

Current Status Of Pseudococcidae (Hemiptera: Coccoidea) In South Korea, Ah-Ram Son, Soo-Jung Suh

Insecta Mundi

Thirty-one species of mealybugs (Hemiptera: Pseudococcidae) are reported from South Korea based upon monitoring surveys conducted from 2010 to 2016, along with a dichotomous key to separate them. Six species, Nipaecoccus nipae (Maskell), Palmicultor lumpurensis (Takahashi), Planococcus citri (Risso), Pseudococcus dendrobiorum Williams, Pseudococcus longispinus (Targioni-Tozzetti), and Pseudococcus orchidicola Takahashi, known only to occur in greenhouses, are considered as adventive mealybug species to South Korea; of these, P. citri has already established in the exterior environment in South Korea.

Description Of The Female Of Solenoptera Tomentosa Lingafelter, 2015 (Coleoptera: Cerambycidae: Prioninae: Solenopterini), Sergio Devesa, Elier Fonseca, Alejandro Barro

Insecta Mundi

The female of Solenoptera tomentosa Lingafelter, 2015 (Coleoptera: Cerambycidae: Prioninae: Solenopterini) is described and illustrated for the first time.

La hembra de Solenoptera tomentosa Lingafelter, 2015 (Coleoptera: Cerambycidae: Prioninae: Solenopterini) es descrita e ilustrada por primera vez.

Descripción De Un Nuevo Género Y Especie De Longicornio Áptero De Perú (Coleoptera: Cerambycidae), Cristian Pineda, Mauricio Cid-Arcos

Insecta Mundi

Se describe e ilustra Polytuberotum metachroma Pineda y Cid-Arcos gen. nov. y sp. nov. (Coleoptera: Cerambycidae) proveniente de Perú. El nuevo género es fácilmente distinguible de Tuberolamia Breuning por su frente transversa y pronoto con espinas laterales y protuberancias discales.

Polytuberotum metachroma Pineda and Cid-Arcos gen. nov. and sp. nov. (Coleoptera: Cerambycidae) from Peru is described and illustrated. The new genus is easily distinguished from Tuberolamia Breuning by its transverse frons and pronotum with lateral spines and discal protuberances.

Description Of A New Species Of Adesmus Lepeletier And Audinet-Serville, 1825, Displaying Chromatic Dimorphism, From Bolivia (Coleoptera, Cerambycidae, Lamiinae, Hemilophini), James E. Wappes, Antonio Santos-Silva

Insecta Mundi

Adesmus martinsi (Coleoptera, Cerambycidae, Lamiinae, Hemilophini), a new species from Bolivia, is described, illustrated, and included in a previous key. The new species displays gender dimorphism in the pubescent pattern and in anatomical structure. Thus, Adesmus becomes the second genus recorded in the Hemilophini to have visual chromatic dimorphism.

A Preliminary Catalogue Of The Moths (Lepidoptera Except Papilionoidea) Of Tobago, West Indies, Matthew J. W. Cock

Insecta Mundi

This catalogue comprises records of 355 species of moths (non-papilionoid Lepidoptera) from Tobago, of which 15 are partially identified. Of this total, all except 17 (5%) are known from Trinidad, although not all these records from Trinidad are published yet. Of these 17, eleven are expected to occur in Trinidad as they also occur on the mainland of South America and two are only known from Tobago but will probably also occur in Trinidad. This leaves just four species (1% of the total) that are known from the Lesser Antilles and are currently not known from further south than Tobago. …

Contribution To The Knowledge Of Chilean Gelechiidae (Lepidoptera: Gelechioidea), Danilo E. Cepeda

Insecta Mundi

A synopsis of the Chilean Gelechiidae (Lepidoptera: Gelechioidea) is presented, including an annotated list of species with geographic distributions and a compilation of previous references. New distribution records for four species are provided.

Spanish abstract

Se presenta una sinopsis de los Gelechiidae de Chile (Lepidoptera: Gelechioidea), que incluye un listado, con su distribución geográfica y una recopilación de antecedentes bibliográficos previos. Se entregan nuevos registros de distribución para cuatro especies.

Monitoring Energy Expenditure Using A Multi-Sensor Device—Applications And Limitations Of The Sensewear Armband In Athletic Populations, Karsten Koehler, Clemens Drenowatz

Department of Nutrition and Health Sciences: Faculty Publications

In order to monitor their energy requirements, athletes may desire to assess energy expenditure (EE) during training and competition. Recent technological advances and increased customer interest have created a market for wearable devices that measure physiological variables and bodily movement over prolonged time periods and convert this information into EE data. This mini-review provides an overview of the applicability of the SenseWear armband (SWA), which combines accelerometry with measurements of heat production and skin conductivity, to measure total daily energy expenditure (TDEE) and its components such as exercise energy expenditure (ExEE) in athletic populations. While the SWA has been shown …

Quantifying Effectiveness Of Streambank Stabilization Practices On Cedar River, Nebraska, Naisargi Dave, Aaron R. Mittelstet

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Excessive sediment is a major pollutant to surface waters worldwide. In some watersheds, streambanks are a significant source of this sediment, leading to the expenditure of billions of dollars in stabilization projects. Although costly streambank stabilization projects have been implemented worldwide, long-term monitoring to quantify their success is lacking. There is a critical need to document the long-term success of streambank restoration projects. The objectives of this research were to (1) quantify streambank retreat before and after the stabilization of 18 streambanks on the Cedar River in North Central Nebraska, USA; (2) assess the impact of a large flood event; …

Self-Assembled Monolayers And Methods For Using The Same In Biosensing Applications, Patrick Dussault, Rebecca Lai, Thomas J. Fisher, Anita J. Zaitouna

Patrick Dussault Publications

Cross - linked amphiphile constructs that form self - as sembled monolayers ( SAMs ) on metal surfaces such as gold surfaces are disclosed . These new SAMs generate well packed and highly oriented monolayer films on gold sur faces . A method for using the SAMs in the fabrication of biomolecule sensors is also disclosed .

Applying Topographic Classification, Based On The Hydrological Process, To Design Habitat Linkages For Climate Change, Yongwon Mo, Dong Kun Lee, Keunyea Song, Ho Gul Kim, Soo Jin Park

School of Natural Resources: Faculty Publications

The use of biodiversity surrogates has been discussed in the context of designing habitat linkages to support the migration of species affected by climate change. Topography has been proposed as a useful surrogate in the coarse-filter approach, as the hydrological process caused by topography such as erosion and accumulation is the basis of ecological processes. However, some studies that have designed topographic linkages as habitat linkages, so far have focused much on the shape of the topography (morphometric topographic classification) with little emphasis on the hydrological processes (generic topographic classification) to find such topographic linkages. We aimed to understand whether …

Experimental Analysis Of Reintroduction Strategies To Conserve The Vulnerable Plains Topminnow Fundulus Sciadicus In Nebraska, David A. Schumann, W. Wyatt Hoback, Keith D. Koupal, Casey W. Schoenebeck, Steven C. Schainost, Tammy L. Wilson

Nebraska Game and Parks Commission: Staff Research Publications

Plains topminnow Fundulus sciadicus are endemic to the Great Plains (USA), and because of declines in their geographic range and local abundance, are granted protection throughout their native range. Experimental reintroductions were conducted to improve conservation techniques and enhance the long-term outlook for plains topminnow persistence in Nebraska. Reintroductions were attempted at 17 extirpated stream reaches using low and high founder densities (no. of fish per suitable area) and during 2 seasons (spring and fall) to identify successful techniques for future conservation efforts. Reintroduced populations were sampled monthly (excluding winter) for 2 yr to monitor population persistence. Repeated presence− absence …

Bioinformatic And Biophysical Analyses Of Proteins, Jonathan Catazaro

Department of Chemistry: Dissertations, Theses, and Student Research

The prevailing dogma in structural genomics is the existence of a strong correlation between protein sequence, structure, and biological function. Proteins with high sequence similarity typically have a similar, if not the same, structure and function. In many cases this logic can fail due to distantly related proteins having very low sequence similarity, a lack of a representative structure, structural novelty, or the absence of a characterized function. Further, the paradigm fails to account for dynamics, which have a significant effect on structural stability and enzymatic efficacy.

Nuclear magnetic resonance (NMR) spectroscopy is uniquely capable of solving the structure, assisting …

Development Of A Broadly Protective Modified-Live Virus Vaccine Candidate Against Porcine Reproductive And Respiratory Syndrome Virus, Haiyan Sun, Aspen Workman, Fernando A. Osorio, David J. Steffen, Hiep L.X. Vu

Nebraska Center for Virology: Faculty Publications

Modified-live virus (MLV) vaccines are widely used to protect pigs against porcine reproductive and respiratory syndrome virus (PRRSV). However, current MLV vaccines do not confer adequate levels of heterologous protection, presumably due to the substantial genetic diversity of PRRSV isolates circulating in the field. To overcome this genetic variation challenge, we recently generated a synthetic PRRSV strain containing a consensus genomic sequence of PRRSV-2. We demonstrated that our synthetic PRRSV strain confers unprecedented levels of heterologous protection. However, the synthetic PRRSV strain at passage 1 (hereafter designated CON-P1) is highly virulent and therefore, is not suitable to be used as …

Evaluating Mobile-Based Citizen Science In Increasing Citizen Participants In Environmental Management, Wenqi Ou

Community and Regional Planning Program: Theses

Mobile-based citizen science as thriving citizen education tool increases the non-expert citizen involvement in the scientific world by encouraging the public upload nature observation to assist scientific research while learning scientific knowledge. Mobile-base citizen science as social media has potential to facilitate citizen engagement in the environmental management. Based on the conceptual framework of citizen science incorporation with environmental management, large users in the program foster the citizen involvement in the environmental management. Research here applies SWOT (Strength, Weakness, Opportunity, Threats) analysis to evaluate mobile based citizen science regarding data management, citizen participation, and partnership. It revealed the current status …

Synthesis Of Graphene And Graphene-Based Composite Membrane, Yuanjun Fan

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

Vibration membrane equipped for earphone requires high performance in both mechanical properties and electronic properties. With extraordinary properties on both, graphene and graphene-based composite materials appear as a promising candidate for this application. Chemical vapor deposition (CVD) is believed to be the most convenient way to synthesize a large area (on scale of square centimeters) as well as a homogeneous thickness for the membrane. The thesis focuses on applying control variable experiment method to analyze different effects on mechanical property of the two CVD setting parameters: cooling rate, and hydrocarbon precursor. For isolating the specimens efficiently, a modified electrochemical method …

Suitability Of River Delta Sediment As Proppant, Missouri And Niobrara Rivers, Nebraska And South Dakota, 2015, Ronald B. Zelt, Christopher M. Hobza, Bethany L. Burton, Nathaniel J. Schaepe, Nadine M. Piatak-Hackley

United States Geological Survey: Water Reports and Publications

Document abstract

Sediment management is a challenge faced by reservoir managers who have several potential options, including dredging, for mitigation of storage capacity lost to sedimentation. As sediment is removed from reservoir storage, potential use of the sediment for socioeconomic or ecological benefit could potentially defray some costs of its removal. Rivers that transport a sandy sediment load will deposit the sand load along a reservoir-headwaters reach where the current of the river slackens progressively as its bed approaches and then descends below the reservoir water level. Given a rare combination of factors, a reservoir deposit of alluvial sand has …

Linking Taxonomic Diversity And Trophic Function: A Graph-Based Theoretical Approach, Marcella M. Jurotich, Kaitlyn Dougherty, Barbara Hayford, Sally Clark

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

The purpose of this study is to develop a novel, visual method in analyzing complex functional trait data in freshwater ecology. We focus on macroinvertebrates in stream ecosystems under a gradient of habitat degradation and employ a combination of taxonomic and functional trait diversity analyses. Then we use graph theory to link changes in functional trait diversity to taxonomic richness and habitat degradation. We test the hypotheses that: 1) taxonomic diversity and trophic functional trait diversity both decrease with increased habitat degradation; 2) loss of taxa leads to a decrease in trophic function as visualized using a bipartite graph; and …

Quaternary Sea-Level History And The Origin Of The Northernmost Coastal Aeolianites In The Americas: Channel Islands National Park, California, Usa, Daniel R. Muhs, Jeffrey S. Pigati, R. Randall Schumann, Gary L. Skipp, Naomi Porat, Stephen B. Devogel

United States Geological Survey: Staff Publications

Along most of the Pacific Coast of North America, sand dunes are dominantly silicate-rich. On the California Channel Islands, however, dunes are carbonate-rich, due to high productivity offshore and a lack of dilution by silicate minerals. Older sands on the Channel Islands contain enough carbonate to be cemented into aeolianite. Several generations of carbonate aeolianites are present on the California Channel Islands and represent the northernmost Quaternary coastal aeolianites on the Pacific Coast of North America. The oldest aeolianites on the islands may date to the early Pleistocene and thus far have only been found on Santa Cruz Island. Aeolianites …

Classic Textiles From Cueva Del Lazo (Chiapas, Mexico): Archaeological Context And Conservation Issues, Davide Domenici, Gloria Martha Sánchez Valenzuela

PreColumbian Textile Conference VII / Jornadas de Textiles PreColombinos VII (2016)

The excavation of Cueva del Lazo, a cave on the cliffs of the Río La Venta River canyon in the Zoque region of Western Chiapas (Mexico), led to the discovery of a complex Late Classic post-sacrificial context where eleven children were buried wrapped in textile bundles. The environmental conditions of the dry cave allowed an extraordinary preservation of perishable materials including textiles, cordages, botanical remains, etc. In the first part of the paper we provide a brief introduction to the archaeology of the Selva El Ocote, as well as a description and interpretation of the Cueva del Lazo archaeological context. …

Mesoamerican Archaeological Textiles: An Overview Of Materials, Techniques, And Contexts, Laura Filloy Nadal

PreColumbian Textile Conference VII / Jornadas de Textiles PreColombinos VII (2016)

In Mesoamerica, unlike the Andean area, there are few instances when climatic conditions allow the preservation of the organic raw materials constituting ancient fabrics. Examples of such textiles conserved in Mexican museums are quite limited, thus even the tiniest fragments recovered in archaeological contexts greatly augment our understanding of ancient weaving technologies and their use in Mesoamerican societies. Most of the surviving fabrics come from dry caves in northern Mexico, but in recent days the exploration of relatively inaccessible rock shelters in southern Mexico has led to the recovery of additional textiles from early times that are associated with funerary …

Efficacy Of An Adenoviral Vectored Multivalent Centralized Influenza Vaccine, Amy Lingel, Brianna L. Bullard, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Mice were immunized with Adenovirus expressing the H1-con, H2-con, H3-con and H5-con HA consensus genes in combination (multivalent) and compared to mice immunized with the traditional 2010–2011 FluZone and FluMist seasonal vaccines. Immunized mice were challenged with 10–100 MLD50 of H1N1, H3N1, H3N2 and H5N1 influenza viruses. The traditional vaccines induced robust levels of HA inhibition (HI) titers, but failed to protect against five different heterologous lethal influenza challenges. Conversely, the multivalent consensus vaccine (1 × 1010 virus particles (vp)/mouse) induced protective HI titers of ≥40 against 8 of 10 influenza viruses that represent a wide degree of divergence within …

Prompting Deliberation About Nanotechnology: Information, Instruction, And Discussion Effects On Individual Engagement And Knowledge, Lisa M. Pytlikzillig, Myiah J. Hutchens, Peter Muhlberger, Alan Tomkins

Lisa PytlikZillig Publications

Deliberative (and educational) theories typically predict knowledge gains will be enhanced by information structure and discussion. In two studies, we experimentally manipulated key features of deliberative public engagement (information, instructions, and discussion) and measured impacts on cognitive-affective engagement and knowledge about nanotechnology. We also examined the direct and moderating impacts of individual differences in need for cognition and gender. Findings indicated little impact of information (organized by topic or by pro-con relevance). Instructions (prompts to think critically) decreased engagement in Study 1, and increased it in Study 2, but did not impact postknowledge. Group discussion had strong positive benefits for …

Mode And Rate Of Evolution Of Haemosporidian Mitochondrial Genomes: Timing The Radiation Of Avian Parasites, M. Andreína Pacheco, Nubia E. Matta, Gediminas Valkiūnas, Patricia G. Parker, Beatriz Mello, Craig E. Stanley Jr., Miguel Lentino, Maria Alexandra Garcia-Amado, Michael Cranfield, Sergei L. Kosakovsky Pond, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

Haemosporidians are a diverse group of vector-borne parasitic protozoa that includes the agents of human malaria; however, most of the described species are found in birds and reptiles. Although our understanding of these parasites’ diversity has expanded by analyses of their mitochondrial genes, there is limited information on these genes’ evolutionary rates. Here, 114 mitochondrial genomes (mtDNA) were studied from species belonging to four genera: Leucocytozoon, Haemoproteus, Hepatocystis, and Plasmodium. Contrary to previous assertions, the mtDNA is phylogenetically informative. The inferred phylogeny showed that, like the genus Plasmodium, the Leucocytozoon and Haemoproteus genera are not monophyletic groups. Although …

Mitochondrial Reactive Oxygen Species In Lipotoxic Hearts Induces Post-Translational Modifications Of Akap121, Drp1 And Opa1 That Promote Mitochondrial Fission, Kensuke Tsushima, Heiko Bugger, Adam R. Wende, Jamie Soto, Gregory A. Jenson, Austin R. Tor, Rose Mcglauflin, Helena C. Kenny, Yuan Zhang, Rhonda Souvenir, Xiao X. Hu, Crystal L. Sloan, Renata O. Pereira, Vitor A. Lira, Kenneth W. Spitzer, Terry L. Sharp, Kooresh I. Shoghi, Genevieve C. Sparagna, Eva A. Rog-Zielinska, Peter Kohl, Oleh Khalimonchuk, Jean E. Schaffer, E. Dale Abel

Department of Biochemistry: Faculty Publications

Rationale: Cardiac lipotoxicity, characterized by increased uptake, oxidation and accumulation of lipid intermediates, contributes to cardiac dysfunction in obesity and diabetes. However, mechanisms linking lipid overload and mitochondrial dysfunction are incompletely understood.

Objective: To elucidate the mechanisms for mitochondrial adaptations to lipid overload in postnatal hearts in vivo.

Methods and Results: Using a transgenic mouse model of cardiac lipotoxicity overexpressing long-chain acyl-CoA synthetase 1 in cardiomyocytes, we show that modestly increased myocardial fatty acid uptake leads to mitochondrial structural remodeling with significant reduction in minimum diameter. This is associated with increased palmitoyl-carnitine oxidation and increased reactive oxygen species (ROS) generation …