Digital Commons Network^™

Genetic Diversity And Distinctness Of Wild Nebraska Hops And Hop Cultivars (Humulus Lupulus L.), Megan Franklin

Honors Program: Senior Projects (Public)

Background Commercial hop (Humulus lupulus) cultivars that are being grown in the Midwest are not performing as successfully as when they are grown in the Pacific Northwest, the region to which they are adapted. To increase adaptation to the Midwest environment, one strategy is to draw from the genetic pool of wild native Midwest hops, which have developed genes that allow them to grow successfully in this environment. Wild hop plants that are genetically distinct from commercial cultivars are likely to have more adaptations, such as pest/disease resistance and drought tolerance, which can be bred into commercial lines. The …

Czech Immigrants In Nebraska: A Question Of Identity And Assimilation, Katharine Meegan

Honors Program: Senior Projects (Public)

This thesis examines the dynamics of cultural and social assimilation through the experiences of Czech immigrants into Nebraska. The Czechs' long struggle to maintain their ethnic identity has shaped their experiences with assimilation. After a review of assimilation theory, I conclude that the Czech experience with assimilation follows a “straight-line” assimilation model, a progression of assimilation that is complete by the third generation. Their relatively small size, settlement in rural areas, and a strong desire to maintain ethnic identity, as reflected in the formation of Czech language benevolent associations, gymnastic societies, and Czech language newspapers, led to “social” and “structural” …

Review Of Genome-Wide Association Studies: From Polymorphism To Personalized Medicine, Jinliang Yang

Department of Agronomy and Horticulture: Faculty Publications

Genome-wide association studies (GWAS) has become a powerful tool in the area of quantitative genetics to map the relationship between trait and genomic variations. This volume provides a great resource for beginners to learn about the recent advances in GWAS and for domain experts to identify the gaps in the area. The first part of the volume lays out the statistical background of GWAS. I really liked the article by Yang et al., Introduction to Statistical Methods in Genome-Wide Association Studies. In this chapter, the authors talked about the missing heritability issue and introduced ways to calculate heritability using the …

Food Habits Of Imperiled Plains Topminnow And Diet Overlap With Invasive Western Mosquitofish In The Central Great Plains, Joseph Thiessen, Keith D. Koupal, Casey W. Schoenebeck, Julie J. Shaffer

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Plains Topminnow (Fundulus sciadicus) populations have experienced large declines throughout the Central Great Plains, with Western Mosquitofish (Gambusia affinis) introductions suggested as a contributing factor. There are limited studies identifying the food habits of Plains Topminnow and the trophic interactions with Western Mosquitofish. This study sought to determine if a diet overlap exists between the Plains Topminnow and the introduced Western Mosquitofish by identifying the feeding habits of both species. We analyzed diets from lentic and lotic populations of Plains Topminnow captured in August and found lentic topminnows employed a generalist diet while lotic topminnow selected for gastropods. …

2017-2018 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

Sixteen counts were held during the 2017-18 Christmas Bird Count season, which is one more than has ever been done in the past. Although the Crawford count was not done this season, the new Lower Platte River count, centered at the junction of Platteview Road and 204th St. south of Gretna, was added. A total 236 observers took part in at least one count, 26 did two counts, four did three counts, five did four counts and four persons did six counts. However, special recognition goes to Gary Roberts, who did eight counts this season. I am not aware of …

Annual Color Photo Section 2018

Nebraska Bird Review

American White Pelican, Wildlife Safari Park, Cass Co., 17 April 2018. Photo by Sheryl Cooley

Trumpeter Swans, Carter Lake, Douglas Co., 1 Jan. 2016. Photo by Brian Peterson.

Bonaparte's Gull, Pawnee Lake SRA, Lancaster Co., 20 June 2018. Photo by Esa Jarvi.

Adult male Longtailed Duck in winter plumage, Carter Lake, Douglas Co., 20 Nov 2017. Photo by Phil Swanson.

Reddish Egret, Platte River bridge, Alda, Hall Co., 24 June 2018. Photo by John Carlini.

Adult Blackcrowned Night Heron, northeast of Lakeside, Sheridan Co., 3 July 2017. Photo by Thomas Gannon.

Juvenile Yellowcrowned Night Heron, Holmes Lake, Lancaster Co., 24 …

Pathways To Open Access, University Of California Libraries

Copyright, Fair Use, Scholarly Communication, etc.

Pursuant to the University of California (UC) Council of University Librarian’s (CoUL)1 3 August 2017 charge, this Pathways to OA Working Group2 has identified the current universe of Open Access (OA) approaches, and has analyzed the suite of strategies available for effectuating those approaches. Each approach described within this Pathways document offers unique and, in some cases, overlapping challenges, opportunities, and room for experimentation. The strategies examined here create space and freedom for the campuses to pursue both individualized and connected paths toward a large-scale transition to OA—though as we highlight below, collective action on implementing the strategies is likely …

Concussion Competencies: A Framework For School-Based Concussion Management (Flyer), Arthur C. Maerlender, Jonathan Lichtenstein, Jennifer Parent-Nichols

Center for Brain, Biology, and Behavior: Faculty Publications

This volume grew out of many years of clinical practice, research, and program projects. It is the culmination of more than 50 years of work with kids, brains, and schools. While there is research behind the Competencies and the underlying content, the intent was to create a user-friendly manual that cut across levels of responsibility and care. Concussion Competencies was not intended to be a textbook in the traditional manner; however, the Competencies have been shown to be a useful approach to teaching this material.

This volume is organized around a set of Competencies that have been shown to be …

An Epigenetic Breeding System In Soybean For Increased Yield And Stability, Sunil K. Kenchanmane Raju, Mon-Ray Shao, Robersy Sanchez, Ying-Zhi Xu, Ajay Sandhu, George L. Graef, Sally A. Mackenzie

Department of Agronomy and Horticulture: Faculty Publications

Epigenetic variation has been associated with a wide range of adaptive phenotypes in plants, but there exist few direct means for exploiting this variation. RNAi suppression of the plant-specific gene, MutS HOMOLOG1 (MSH1), in multiple plant species produces a range of developmental changes accompanied by modulation of defence, phytohormone and abiotic stress response pathways along with methylome repatterning. This msh1-conditioned developmental reprogramming is retained independent of transgene segregation, giving rise to transgene-null ‘memory’ effects. An isogenic memory line crossed to wild type produces progeny families displaying increased variation in adaptive traits that respond to selection. This study …

Electron Effective Mass In In0.33ga0.67n Determined By Mid- Infrared Optical Hall Effect, Nerijus Armakavicius, Vallery Stanishev, Sean Knight, Mathias Schubert, Vanya Darakchieva

Department of Electrical and Computer Engineering: Faculty Publications

Mid-infrared optical Hall effect measurements are used to determine the free charge carrier parameters of an unintentionally doped wurtzite-structure c-plane oriented In_0.33Ga_0.67N epitaxial layer. Room temperature electron effective mass parameters of m_ ¼ ð0:20560:013Þm0 andm_k ¼ ð0:20460:016Þm0 for polarization perpendicular and parallel to the c-axis, respectively,were determined. The free electron concentration was obtained as (1.760.2)_1019 cm^-3. Within our uncertainty limits, we detect no anisotropy for the electron effective mass parameter and we estimate the upper limit of the possible effective mass anisotropy as 7%. We discuss the influence of conduction band nonparabolicity on …

Sarcocystis Cymruensis: Discovery In Western Hemisphere In The Brown Rat (Rattus Norvegicus) From Grenada, West Indies: Redescription, Molecular Characterization, And Transmission To Ifn-Γ Gene Knockout Mice Via Sporocysts From Experimentally Infected Domestic Cat (Felis Catus), Fernando H. Antunes Murata, Camila K. Cerqueira-Cézar, Peter C. Thompson, Keshaw Tiwari, Joseph D. Mowery, Shiv K. Verma, Benjamin M. Rosenthal, Ravindra N. Sharma, J. P. Dubey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Rodents are intermediate hosts for many species of Sarcocystis. Little is known of Sarcocystis cymruensis that uses the Brown rat (Rattus norvegicus) as intermediate hosts and the domestic cat (Felis catus) as experimental definitive host. Here, we identified and described Sarcocystis cymruensis in naturally infected R. norvegicus from Grenada, West Indies. Rats (n = 167) were trapped in various locations in two parishes (St. George and St. David). Microscopic, thin (< 1 μm) walled, slender sarcocysts were found in 11 of 156 (7.0%) rats skeletal muscles by squash examination. A laboratory-raised cat fed naturally infected rat tissues excreted sporocysts that were infectious for interferon gamma gene knockout (KO) mice, but not to Swiss Webster outbred albino mice. All inoculated mice remained asymptomatic, and microscopic S. cymruensis-like sarcocysts were found in the muscles of KO mice euthanized on day 70, 116, and 189 post inoculation (p.i.). Sarcocysts from infected KO mice were infective for cats at day 116 but not at 70 days p.i. By transmission electron microscopy, the sarcocyst wall was Btype 1a.^ Detailed morphological description of the cyst wall, metrocytes, and bradyzoites is given for the first time. Additionally, molecular data on S. cymruensis are presented also for the first time. Molecular characterization of sarcocysts 18S rDNA and 28S rDNA, ITS-1, and cox1 loci showed the highest similarity with S. rodentifelis and S. muris. In conclusion, the present study described the natural infection of S. cymruensis in Brown rat for the first time in a Caribbean country and provided its molecular characteristics.

Description Of A New Species Of Ochraethes Chevrolat, 1860, Redescription Of Plocaederus Mirim Martins And Monné, 2002, And New Geographical Records For Plocaederus Dejean, 1835 (Coleoptera, Cerambycidae, Cerambycinae), James E. Wappes, Antonio Santos-Silva, Juan Pablo Botero

Insecta Mundi

A new Mexican species of Ochraethes Chevrolat, 1860 (Coleoptera, Cerambycidae, Cerambycinae, Clytini) is described: Ochraethes skillmani Wappes, Santos-Silva and Botero. Plocaederus mirim Martins and Monné, 2002 (Cerambycini) is redescribed and its female is figured for the first time. New geographical records in Plocaederus Dejean, 1835 are also provided.

A New Synonymy And Transference In Cerambycinae (Coleoptera, Cerambycidae), James E. Wappes, Antonio Santos-Silva

Insecta Mundi

Rhinion parkeri Wappes and Santos-Silva, 2017 is synonymized with Saltanecydalopsis irwini Barriga and Cepeda, 2007 (Coleoptera, Cerambycidae), and the genus is transferred to Rhinotragini Thomson, 1861 from Necydalopsini Lacordaire, 1868.

Dasymutilla Ashmead (Hymenoptera, Mutillidae) In Panama: New Species, Sex Associations And Seasonal Flight Activity, Roberto A. Cambra, Kevin A. Williams, Diomedes Quintero, Donald M. Windsor, John Pickering, Daisy Saavedra

Insecta Mundi

A taxonomic revision of Panamanian species of the genus Dasymutilla Ashmead (Hymenoptera, Mutillidae) is presented and a key for the six species is given, all recognized from both sexes. Dasymutilla colorado Cambra, Williams and Quintero sp. nov., from central and eastern Panama, is described and illustrated. Sex associations permitted us to make the following five synonymies: D. sleipniri Manley and Pitts, 2007 (male) under D. phya (Cameron, 1895) (female); D. deyrollesi Mickel, 1937 (male) and Sphaerophthama [sic.] temaxensis Cameron, 1895 under Dasymutilla araneoides (Smith, 1862) (female); D. ionothorax Manley and Pitts, 2007 (male) under Dasymutilla spilota Manley and Pitts, …

Seroprevalence Survey For Microsporidia In Common Bottlenose Dolphin (Tursiops Truncatus): Example Of A Quantitative Approach Based On Immunoblotting, Guillaume Desoubeaux, Roman Peschke, Carolina Le-Bert, Vanessa Fravel, Jeny Soto, Eric D. Jensen, Jennifer E. Flower, Randall Wells, Anja Joachim, Carolyn Cray

United States Navy: Publications

Little is known about microsporidiosis pathogenicity in cetaceans. Here we report seroprevalence of 76% for microsporidia in blood samples from common bottlenose dolphins (Tursiops truncatus), from animals managed under human care (n=108) or captured for health assessments (n=13) and released.

Gamma Irradiation Inactivates Honey Bee Fungal, Microsporidian, And Viral Pathogens And Parasites, Michael Simone-Finstrom, Kate Aronstein, Michael Goblirsch, Frank Rinkevich, Lilia De Guzman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Managed honey bee (Apis mellifera) populations are currently facing unsustainable losses due to a variety of factors. Colonies are challenged with brood pathogens, such as the fungal agent of chalkbrood disease, the microsporidian gut parasite Nosema spp., and several viruses. These pathogens may be transmitted horizontally from worker to worker, vertically from queen to egg and via vectors like the parasitic mite, Varroa destructor. Despite the fact that these pathogens are widespread and often harbored in wax comb that is reused from year to year and transferred across beekeeping operations, few, if any, universal treatments exist for their control. In …

Early Tcr Signaling Induces Rapid Aerobic Glycolysis Enabling Distinct Acute T Cell Effector Functions, Ashley V. Menk, Nicole E. Scharping, Rebecca S. Moreci, Xue Zeng, Cliff Guy, Sonia Salvatore, Heekyong Bae, Jianxin Xie, Howard A. Young, Stacy Gelhaus Wendell, Greg M. Delgoffe

United States Public Health Resources

To fulfill bioenergetic demands of activation, T cells perform aerobic glycolysis, a process common to highly proliferative cells in which glucose is fermented into lactate rather than oxidized in mitochondria. However, the signaling events that initiate aerobic glycolysis in T cells remain unclear. We show T cell activation rapidly induces glycolysis independent of transcription, translation, CD28, and Akt and not involving increased glucose uptake or activity of glycolytic enzymes. Rather, TCR signaling promotes activation of pyruvate dehydrogenase kinase 1 (PDHK1), inhibiting mitochondrial import of pyruvate and facilitating breakdown into lactate. Inhibition of PDHK1 reveals this switch is required acutely for …

Nathan Augustus Cobb: The Father Of Nematology In The United States; And Nathan Cobb's Publications, R. N. Huettel, A. Morgan Golden, Donna Ellington

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Introduction

Nathan Augustus Cobb, referred to as the "Father of Nematology in the United States" (56), was a Renaissance man and a man of humble beginnings. His early life experiences instilled in him the creativity, fortitude, and self reliance that were needed to raise himself alone from age 14, to complete college with little formal pre-college education, receive his Ph.D. in Germany, and go on to found a new discipline of science called "Nematology". His scientific and technical accomplishments were impressive, as indicated by the over 250 first-authored manuscripts he produced during his career. He identified over 1000 species of …

Mac3a And Mac3b, Two Core Subunits Of The Mos4-Associated Complex, Positively Influence Mirna Biogenesis, Shengjun Li, Kan Liu, Bangjun Zhou, Mu Li, Shuxin Zhang, Lirong Zeng, Chi Zhang, Bin Yu

Center for Plant Science Innovation: Faculty Publications

MAC3A and MAC3B are conserved U-box containing proteins in eukaryotes. They are subunits of the MOS4-associated complex (MAC) that plays essential roles in plant immunity and development in Arabidopsis. However, their functional mechanisms remain elusive. Here we show that Arabidopsis thaliana MAC3A and MAC3B act redundantly in microRNA (miRNA) biogenesis. Lack of both MAC3A and MAC3B in the mac3b mac3b double mutant reduces the accumulation of miRNAs, causing elevated transcript levels of miRNA targets. mac3a mac3b also decreases the levels of primary miRNA transcripts (pri-miRNAs). However, MAC3A and MAC3B do not affect the promoter activity of genes encoding miRNAs ( …

Temperature Drives Epidemics In A Zooplankton-Fungus Disease System: A Trait-Driven Approach Points To Transmission Via Host Foraging, Marta S. Shocket, Alexander T. Strauss, Jessica L. Hite, Maja Šljivar, David J. Civitello, Meghan A. Duffy, Carla E. Cáceres, Spencer R. Hall

School of Biological Sciences: Faculty Publications

Climatic warming will likely have idiosyncratic impacts on infectious diseases, causing some to increase while others decrease or shift geographically. A mechanistic framework could better predict these different temperature-disease outcomes. However, such a framework remains challenging to develop, due to the nonlinear and (sometimes) opposing thermal responses of different host and parasite traits and due to the difficulty of validating model predictions with observations and experiments. We address these challenges in a zooplanktonfungus (Daphnia dentifera–Metschnikowia bicuspidata) system. We test the hypothesis that warmer temperatures promote disease spread and produce larger epidemics. In lakes, epidemics that start earlier and warmer in …

Manuscript Preparation Rules And Guidelines, February 2018

Insecta Mundi

Insecta Mundi is an ongoing experiment in publishing. Our goal is to produce taxonomic publications that are free for authors (no page charges) and openly accessible for users (see Skelley and Thomas 2007). To accomplish this, we require the participation of authors in the publication process. Authors are required to make their manuscripts publishable by our standards prior to submission. Authors unwilling or unable to follow the guidelines should ask friends for help or consider paying to have their article published in journals which charge for the services.

The purposes for these rules and guidelines are to ensure consistency in …

Inheritance Of Mesotrione Resistance In An Amaranthus Tuberculatus (Var. Rudis) Population From Nebraska, Usa, Maxwel C. Oliveira, Todd A. Gaines, Amit J. Jhala, Stevan Z. Knezevic

Department of Agronomy and Horticulture: Faculty Publications

A population of Amaranthus tuberculatus (var. rudis) evolved resistance to 4- hydroxyphenylpyruvate dioxygenase (HPPD) inhibitor herbicides (mesotrione, tembotrione, and topramezone) in Nebraska. The level of resistance was the highest to mesotrione, and the mechanism of resistance in this population is metabolism-based likely via cytochrome P450 enzymes. The increasing number of weeds resistant to herbicides warrants studies on the ecology and evolutionary factors contributing for resistance evolution, including inheritance of resistance traits. In this study, we investigated the genetic control of mesotrione resistance in an A. tuberculatus population from Nebraska, USA. Results showed that reciprocal crosses in the F1 families exhibited …

Present And Future Thermal Environments Available To Sharp-Tailed Grouse In An Intact Grassland, Edward J. Raynor, Larkin Powell, Walter H. Schacht

School of Natural Resources: Faculty Publications

Better understanding animal ecology in terms of thermal habitat use has become a focus of ecological studies, in large part due to the predicted temperature increases associated with global climate change. To further our knowledge on how ground-nesting endotherms respond to thermal landscapes, we examined the thermal ecology of Sharp-tailed Grouse (Tympanuchus phasianellus) during the nesting period. We measured site-specific iButton temperatures (TiB) and vegetation characteristics at nest sites, nearby random sites, and landscape sites to assess thermal patterns at scales relevant to nesting birds. We asked if microhabitat vegetation characteristics at nest sites matched the characteristics that directed macrohabitat …

Hexapod Herald - Vol. 30, No. 1, February 2018

Hexapod Herald and Other Entomology Department Newsletters

Contents: Welcome - Congratulations - Faculty News - Gamma Sigma Delta Awards - Publications - Blast from the Past - Baxendale Retirement Reception

Effects Of Elevated Co2, Increased Nitrogen Deposition, And Plant Diversity On Aboveground Litter And Root Decomposition, Xiaoan Zuo, Johannes Knops

School of Biological Sciences: Faculty Publications

Global change-induced litter decomposition strongly affects the carbon (C) and nitrogen (N) dynamics in grassland ecosystems. However, few studies show the interactive effects of global change factors on litter and root decomposition. We conducted a four-year grassland field experiment to examine the quality and decomposition of litter and root in a three-factorial experiment with elevated CO₂, increased N deposition, and plant species richness. We found that elevated CO₂ decreased the litter N content and root lignin content. N addition increased the root N content and decreased the litter lignin content. Increasing plant richness decreased the N and …

In Their Own Words: The Significance Of Participant Perceptions In Assessing Entomology Citizen Science Learning Outcomes Using A Mixed Methods Approach, Louise I. Lynch, Jenny Dauer, Wayne A. Babchuk, Tiffany Heng-Moss, Doug Golick

Department of Entomology: Faculty Publications

A mixed methods study was used to transcend the traditional pre-, post-test approach of citizen science evaluative research by integrating adults’ test scores with their perceptions. We assessed how contributory entomology citizen science affects participants’ science self-efficacy, self-efficacy for environmental action, nature relatedness and attitude towards insects. Pre- and post-test score analyses from citizen scientists (n = 28) and a control group (n = 72) were coupled with interviews (n = 11) about science experiences and entomological interactions during participation. Considering quantitative data alone, no statistically significant changes were evident in adults following participation in citizen science when compared to …

Brain–Computer Interfaces For Augmentative And Alternative Communication: A Tutorial, Jonathan S. Brumberg, Kevin M. Pitt, Alana Mantie-Kozlowski, Jeremy D. Burnison

Department of Special Education and Communication Disorders: Faculty Publications

Purpose--Brain–computer interfaces (BCIs) have the potential to improve communication for people who require but are unable to use traditional augmentative and alternative communication (AAC) devices. As BCIs move toward clinical practice, speech-language pathologists (SLPs) will need to consider their appropriateness for AAC intervention.

Method--This tutorial provides a background on BCI approaches to provide AAC specialists foundational knowledge necessary for clinical application of BCI. Tutorial descriptions were generated based on a literature review of BCIs for restoring communication.

Results--The tutorial responses directly address 4 major areas of interest for SLPs who specialize in AAC: (a) the current state …

Acoustic Surveys Of Bats At Northern Great Plains Parks And Preliminary Results From 2014-16, Daniel S. Licht

United States National Park Service: Publications

Executive Summary

From 2014-16, the Northern Great Plains Inventory & Monitoring Network (Network)—in collaboration with the Midwest Regional Biologist—used acoustic methods to monitor bat populations at 12 Network parks. Six parks were monitored using the nascent North American Bat Monitoring Program (NABat) framework. Six other parks did not meet a priori criteria for inclusion in NABat so they were monitored using similar methods, but a non-systematic sampling frame.

Fifty-five NABat stations were established along with 62 non-NABat stations. Stations were typically monitored for 4-7 nights each year using equipment that records the echolocation calls of bats. Fourteen mobile survey routes …

Investigation Of Cry3bb1 Resistance And Intoxication In Western Corn Rootworm By Rna Sequencing, Leslie Catherine Rault, Blair D. Siegfried, Aaron J. Gassmann, Haichuan Wang, Gary J. Brewer, Nicholas J. Miller

Department of Entomology: Faculty Publications

The western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte is a major pest of corn that has evolved resistance to transgenic maize that produces insecticidal Cry toxins. The specific mode of action of Cry3Bb1 and mechanism of resistance in WCR are unknown. This study compared gene expression between Cry3Bb1-susceptible and Cry3Bb1-resistant WCR neonates, in the presence and absence of Cry3Bb1. RNA-Seq data were analyzed to identify differentially expressed transcripts between strains of WCR, providing candidate transcripts for resistance to Cry3Bb1. Constitutive and Cry3Bb1-induced differences between strains caused the differential expression of 608 transcripts after 8 hr. Differentially expressed transcripts between …

Mute Swans, David R. Marks

Wildlife Damage Management Technical Series

Mute swans (Cygnus olor) are an invasive species originally brought to the United States in the late 19th and early 20th centuries for ornamental ponds and lakes, zoos and aviculture collections. Original populations were located in northeastern states along the Hudson Valley but have since expanded to several Midwestern states and portions of the western U.S. and Canada. Mute swan damage includes competing with native waterfowl, destroying native plants, spreading disease, and colliding with aircraft. They are also considered a nuisance in some areas due to their abundant fecal droppings and aggressiveness towards people.

Mute swans can impact …