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Tissue‑ And Time‑Dependent Metabolite Profiles During Early Grain Development Under Normal And High Night‑Time Temperature Conditions, Nathan Abshire, Andrew L. Hauck, Harkamal Walia, Toshihiro Obata

Department of Agronomy and Horticulture: Faculty Publications

Background Wheat grain development in the first few days after pollination determines the number of endosperm cells that influence grain yield potential and is susceptible to various environmental conditions, including high night temperatures (HNTs). Flag leaves and seed-associated bracts (glumes, awn, palea, and lemma) provide nutrients to the developing seed. However, the specific metabolic roles of these tissues are uncertain, especially their dynamics at different developmental stages and the time in a day. Tissue- and time-dependent metabolite profiling may hint at the metabolic roles of tissues and the mechanisms of how HNTs affect daytime metabolic status in early grain development. …

Structural And Biophysical Insights Into Targeting Of Claudin-4 By A Synthetic Antibody Fragment, Satchal K. Erramilli, Pawel K. Dominik, Chinemerem P. Ogbu, Anthony A. Kossiakoff, Alex J. J.

Department of Biochemistry: Faculty Publications

Claudins are a 27-member family of ~25 kDa membrane proteins that integrate into tight junctions to form molecular barriers at the paracellular spaces between endothelial and epithelial cells. As the backbone of tight junction structure and function, claudins are attractive targets for modulating tissue permeability to deliver drugs or treat disease. However, structures of claudins are limited due to their small sizes and physicochemical properties—these traits also make therapy development a challenge. Here we report the development of a synthetic antibody fragment (sFab) that binds human claudin-4 and the determination of a high-resolution structure of it bound to claudin-4/enterotoxin complexes …

Perspectives On Computational Modeling Of Biological Systems And The Significance Of The Sysmod Community, Bhanwar Lal Puniya, Meghna Verma, Chiara Damiani, Shaimaa Bakr, Andreas Dräger

Department of Biochemistry: Faculty Publications

Motivation: In recent years, applying computational modeling to systems biology has caused a substantial surge in both discovery and practical applications and a significant shift in our understanding of the complexity inherent in biological systems.

Results: In this perspective article, we briefly overview computational modeling in biology, highlighting recent advancements such as multiscale modeling due to the omics revolution, single-cell technology, and integration of artificial intelligence and machine learning approaches. We also discuss the primary challenges faced: integration, standardization, model complexity, scalability, and interdisciplinary collaboration. Lastly, we highlight the contribution made by the Computational Modeling of Biological Systems …

Sensitization Of Europium Oxide Nanoparticles Enhances Signal-To- Noise Over Autofluorescence With Time-Gated Luminescence Detection, Hunter Miller, Jessica Q. Wallace, Hui Li, Xing-Zhong Li, Ana De Bettencourt-Dias, Forrest Kievit

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Clinical translation of nanoparticle-based therapeutics has been limited, and a lack of preclinical delivery characterization is partly to blame, limiting our understanding of the mechanisms of failure. The improvement of the preclinical delivery assessment requires nanoparticles with higher detectability. This work focused on the exploration of several aromatic carboxylic ligands (terephthalic acid, quinaldic acid, and kynurenic acid) for the sensitization of europium oxide nanoparticles with a long emission lifetime to overcome cellular autofluorescence, a key confounder of detection in luminescence-based bioimaging. A facile one-pot synthesis and ligand exchange process generated and sensitized ultrasmall Eu₂O₃ cores. As reflected …

Computable Species Descriptions And Nanopublications: Applying Ontology-Based Technologies To Dung Beetles (Coleoptera, Scarabaeinae), Giulio Montanaro, James P. Balhoff, Jennifer C. Girón, Max Söderholm, Sergei Tarasov

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Taxonomy has long struggled with analysing vast amounts of phenotypic data due to computational and accessibility challenges. Ontology-based technologies provide a framework for modelling semantic phenotypes that are understandable by computers and compliant with FAIR principles. In this paper, we explore the use of Phenoscript, an emerging language designed for creating semantic phenotypes, to produce computable species descriptions. Our case study centers on the application of this approach to dung beetles (Coleoptera, Scarabaeinae).

Winter Gps Tagging Reveals Home Ranges During The Breeding Season For A Borealnesting Migrant Songbird, The Goldencrowned Sparrow, Autumn R. Iverson, Diana L. Humple, Renée L. Cromier, Thomas P. Hahn, Theadora A. Block, Daizaburo Shizuka, Bruce E. Lyon, Alexis S. Chaine, Emily J. Hudson, Elisha M. Hull

School of Biological Sciences: Faculty Publications

Determining space use for species is fundamental to understanding their ecology, and tracking animals can reveal insights into their spatial ecology on home ranges and territories. Recent technological advances have led to GPS-tracking devices light enough for birds as small as ~30 g, creating novel opportunities to remotely monitor fine-scale movements and space use for these smaller species. We tested whether miniaturized GPS tags can allow us to understand space use of migratory birds away from their capture sites and sought to understand both pre-breeding space use as well as territory and habitat use on the breeding grounds. We used …

Production Of The Siderophore Lysochelin In Rich Media Through Maltose-Promoted High-Density Growth Of Lysobacter Sp. 3655, Fang Zhang, Jia Liu, Lin Jiang, Yongbiao Zheng, Lingjun Yu, Liangcheng Du

Department of Chemistry: Faculty Publications

Siderophores are produced by bacteria in iron-restricted conditions. However, we found maltose could induce the biosynthesis of the siderophore lysochelin in Lysobacter sp. 3655 in rich media that are not compatible with siderophore production. Maltose markedly promoted cell growth, with over 300% increase in cell density (OD₆₀₀) when LB medium was added with maltose (LBM). While lysochelin was not detectable when OD₆₀₀ in LBM was below 5.0, the siderophore was clearly produced when OD₆₀₀ reached 7.5 and dramatically increased when OD₆₀₀ was 15.0. Coincidently, the transcription of lysochelin biosynthesis genes was remarkably enhanced following the …

Concepts In Animal Parasitology, Scott L. Gardner, Sue Ann Gardner

Zea E-Books Collection

Note that this version of the textbook has been superceded by the one updated in 2026, available at the UNL Pressbooks site.

The hard copy version that is for sale at lulu.com is the old version.

"This is a textbook covering concepts in animal parasitology. It is meant to be used by students, teachers, professors, researchers, and members of the public who are interested in learning about animal parasite biology, systematics, taxonomy, zoogeography, and ecology. The primary intended audience is upper-level undergraduate or graduate university students who have knowledge of basic biology and, particularly, basic animal biology. (863 pages, …

Tsunami Exposure And Mental Health Consequences: Protective Role Of Cultural Coping Strategies, Thulitha Wickrama, Michael J. Merten, K. A.S. Wickrama, Amanda Terrell

Department of Child, Youth, and Family Studies: Faculty Publications

There is a knowledge gap regarding the link between disaster exposure and adolescent mental health problems in developing countries. This study examines the case of Sri Lanka to investigate (a) the immediate and long-term mental health impact of the 2004 tsunami disaster on adolescents and (b) the potential moderating effects of unique cultural and family practices that prevail in Sri Lanka. This study used a random sample of 160 adolescents (ages 12–19) and their mothers who were exposed to the tsunami disaster while living in a southern Sri Lankan village and provided prospective data immediately after the disaster (2005) and …

Protein Nanoparticles As Vaccine Platforms For Human And Zoonotic Viruses, Kush Kumar Pandey, Bikash R. Sahoo, Asit K. Pattnaik

Nebraska Center for Virology: Faculty Publications

Vaccines are one of the most effective medical interventions, playing a pivotal role in treating infectious diseases. Although traditional vaccines comprise killed, inactivated, or liveattenuated pathogens that have resulted in protective immune responses, the negative consequences of their administration have been well appreciated. Modern vaccines have evolved to contain purified antigenic subunits, epitopes, or antigen-encoding mRNAs, rendering them relatively safe. However, reduced humoral and cellular responses pose major challenges to these subunit vaccines. Protein nanoparticle (PNP)-based vaccines have garnered substantial interest in recent years for their ability to present a repetitive array of antigens for improving immunogenicity and enhancing protective …

Malnutrition Alters Protein Expression Of Kndy Neuropeptides In The Arcuate Nucleus Of Mature Ewes, Jennifer F. Thorson, Ligia D. Prezotto

Department of Animal Science: Faculty Publications

The neuropeptides kisspeptin, neurokinin B, and dynorphin A are imperative for the pulsatile secretion of gonadotropin-releasing hormone and luteinizing hormone to ultimately regulate reproductive cyclicity. A population of neurons co-expressing these neuropeptides, KNDy neurons, within the arcuate nucleus of the hypothalamus (ARC) are positioned to integrate energy status from afferent neuronal and glial cells. We hypothesized that KNDyexpressing neurons in the ARC of mature ewes are influenced by energy balance. To test this hypothesis, ovary-intact, mature ewes were fed to lose, maintain, or gain body weight and hypothalamic tissue harvested during the luteal phase of the estrous cycle. Fluorescent, multiplex …

Sustainable Sensing With Paper Microfluidics: Applications In Health, Environment, And Food Safety, Sanjay Kumar, Jyoti B. Kaushal, Heow Pueh Lee

Durham School of Architectural Engineering and Construction: Faculty Publications

This manuscript offers a concise overview of paper microfluidics, emphasizing its sustainable sensing applications in healthcare, environmental monitoring, and food safety. Researchers have developed innovative sensing platforms for detecting pathogens, pollutants, and contaminants by leveraging the paper’s unique properties, such as biodegradability and affordability. These portable, low-cost sensors facilitate rapid diagnostics and on-site analysis, making them invaluable tools for resource-limited settings. This review discusses the fabrication techniques, principles, and applications of paper microfluidics, showcasing its potential to address pressing challenges and enhance human health and environmental sustainability.

Topological Regression As An Interpretable And Efficient Tool For Quantitative Structureactivity Relationship Modeling, Ruibo Zhang, Daniel Nolte, Cesar Sanchez-Villalobos, Souparno Ghosh, Ranadip Pal

Department of Statistics: Faculty Publications

Quantitative structure-activity relationship (QSAR)modeling is a powerful tool for drug discovery, yet the lack of interpretability of commonly used QSAR models hinders their application inmolecular design.We propose a similaritybased regression framework, topological regression (TR), that offers a statistically grounded, computationally fast, and interpretable technique to predict drug responses. We compare the predictive performance of TR on 530 ChEMBL human target activity datasets against the predictive performance of deep-learning-based QSAR models. Our results suggest that our sparse TR model can achieve equal, if not better, performance than the deep learningbased QSAR models and provide better intuitive interpretation by extracting an approximate …

Less Activity Means Improved Welfare? How Pair Housing Influences Pinyon Jay (Gymnorhinus Cyanocephalus) Behaviour, London M. Wolff, Jeffrey R. Stevens

Center for Brain, Biology, and Behavior: Faculty Publications

The activity level and specific behaviours exhibited by captive animals are crucial indicators of welfare. Stereotypies, or repetitive behaviours that have no apparent function or goal, are performed by animals experiencing poor conditions in their environment and indicate welfare concerns. Changes in the housing environment in particular may have critical influences on behaviour and welfare. Here, we measured behavioural changes in a captive pinyon jay (Gymnorhinus cyanocephalus) population (n = 10) associated with a shift from single to pair housing. Using automated video processing, we show that pair housing greatly reduced overall activity levels in these birds. The …

Nou Spring Field Days And Annual Meeting, 125th Anniversary Of The Nebraska Ornithologists’ Union, Birding Bowl Kickoff Weekend, Lincoln, Mary 3–5, 2024, Janis Paseka

Nebraska Bird Review

The NOU Annual Meeting and Spring Field Days were held in Lincoln on May 3-5, 2024, with 80 in attendance. This year is the 125th anniversary of the founding of the Nebraska Ornithologists’ Union, and this meeting also served as the kickoff of the Nebraska Birding Bowl.

Field trips were led by Olivia DaRugna, Thane Dinsdale, Joe Gubanyi, Thomas Labedz, Jason St. Sauver, T. J. Walker, and Michael Willison. Destinations included Wilderness Park, Bluestem Lake SRA, Killdeer WMA, Cottontail WMA, Pawnee Lake SRA, Branched Oak Lake, Butler County Prairie-Chicken lek, Marsh Wren Saline Wetland, Spring Creek Prairie, and Conestoga Lake …

The Early Years Of The Nebraska Ornithologists’ Union: People And Publications, Daniel W. Leger

Nebraska Bird Review

Nebraska once boasted that it had the greatest avian biodiversity of any state. Today, such a claim seems preposterous, but when it was made, back in 1899, it was serious. The antecedents of that claim underlie the objective of this article, namely to describe the early history of the Nebraska Ornithologists’ Union (NOU), its founding members, and its publications, one of which, the Nebraska Bird Review, you are reading today.

. . .

To say that many changes have occurred in ornithology and birding since the NOU started in 1899 would be a severe understatement. Most of these changes have …

The Nebraska Bird Review (June 2024) 92(2), Whole Issue

Nebraska Bird Review

Spring Field Report, March–May 2024, by W. Ross Silcock

The Early Years of the Nebraska Ornithologists’ Union: People and Publications, by Daniel W. Leger

NOU Spring Field Days and Annual Meeting, 125th Anniversary of the Nebraska Ornithologists’ Union, Birding Bowl Kickoff Weekend, Lincoln, May 3–5, 2024, by Janis Paseka

Subscription and Organization Information

Spring Field Report, March–May 2024, W. Ross Silcock

Nebraska Bird Review

A major concern this spring was the widely lamented shortfall in numbers of migrant warblers and the shortened duration of the migration, with most sightings relatively early. Numbers of migrant warblers east of the 100th meridian were investigated by Joel Jorgensen and Stephen Brenner and the scarcity was indeed found to be supported by eBird data; see the “News and Updates” section in Birds of Nebraska-Online (BONO). At Oliver Reservoir in the Panhandle, however, Steven Mlodinow found that migration was at least normal for a range of common migrants, with some migrants above the levels of the last four years. …

Neuropsychological Intra-Individual Variability In Traumatic Brain Injury: Clinical Utility, Classification, And Associations With Outcomes, Jeremy A. Feiger

Dissertations and Doctoral Documents, University of Nebraska-Lincoln, 2023–

Traumatic brain injury (TBI) survivors experience a range of sequelae including neurobiological brain changes, cognitive impairment, psychological difficulties, and functional changes. Neuropsychological assessment after injury is used to characterize current strengths and weaknesses to inform prognosis and interventions. There is currently no reliable and consistent method of assessing and classifying injuries in the post-acute and chronic phases of moderate to severe TBI (msTBI) that is associated with long term psychosocial outcomes. This study aimed to combine the use of within-person variability on neuropsychological test batteries and in cognitive response latency, known as intra-individual variability (IIV), with a novel cognitive phenotyping …

Geographic Distribution: Smilisca Baudinii (Mexican Treefrog). Usa: Florida: Escambia Co., Louis A. Somma, Justin K. Anto, Jakira A. Davis

Papers in Herpetology

Geographic distribution note for Smilisca baudinii, the Mexican treefrog, from Escambia County, Florida, United States.

Fabrication Of A Low-Cost Drum Seeder For Paddy, Muhammad Rashed Al Mamun, Md Rejaul Karim, Md Towfiqur Rahman, Md Janibul Alam Soeb, Md Imranul Alam

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Rice is a staple food crop. Bangladesh produces it extensively which raised the nation into the fourth-largest rice-grower in the world. Transplanting or direct seeding is a major task in rice cultivation where the manual transplanting method requires a huge labor cost which is beyond control in a peak season. Although mechanical rice transplanters become popularization land preparation and seedling rising as a pre transplanting task is costly and labor-intensive. On the other hand, manual broadcasting and machine seeding direct method still exist due to the above method’s problem. Considering spacing accuracies for intercultural operation, drum seeders are an easy …

An Inaugural Forum On Epidemiological Modeling For Public Health Stakeholders In Arizona, Joseph R. Mihalijevic, Carmenlita Chief, Mehreen Malik, Kayode Oshinubi, Eck Doerry, Esma Gel, Crystal Hepp, Tim Lant, Sanjay Mehrotra, Samantha Sabo

Department of Supply Chain Management and Analytics: Faculty Publications

Epidemiological models—which help us understand and forecast the spread of infectious disease—can be valuable tools for public health. However, barriers exist that can make it difficult to employ epidemiological models routinely within the repertoire of public health planning. These barriers include technical challenges associated with constructing the models, challenges in obtaining appropriate data for model parameterization, and problems with clear communication of modeling outputs and uncertainty. To learn about the unique barriers and opportunities within the state of Arizona, we gathered a diverse set of 48 public health stakeholders for a day-and-a-half forum. Our research group was motivated specifically by …

Morphometric Analysis And Taxonomic Re-Evaluation Of Pepsis Cerberus Lucas And P. Elegans Lepeletier (Hymenoptera: Pompilidae: Pepsinae: Pepsini), Frank E. Kurczewski, Akira Shimizu, Diane H. Kiernan

Insecta Mundi

Hurd (1952) separated Pepsis cerberus Lucas from P. elegans Lepeletier (Hymenoptera: Pompilidae: Pepsinae: Pepsini) based on external morphology and biogeography. Vardy (2005) synonymized the familiar and historically well-documented P. cerberus and P. elegans, combining these Nearctic taxa with several Neotropical variants in an extremely broad definition of P. menechma Lepeletier. In doing so, Vardy (2005) breached the principle of nomenclatural stability. He ignored the prevailing usage and clearly violated articles 23.2, 23.3 and 23.9.1.2 of the ICZN (1999). Morphological differences, ecological divergence, and narrow sympatric geographic distribution of P. cerberus and P. elegans …

A New Species Of Paranthrene Hübner (Lepidoptera: Sesiidae) From The Northern Midwest United States, William H. Smith Iii, William H. Taft Jr., Anthony I. Cognato

Insecta Mundi

A clearwing moth species, Paranthrene sogaardi Taft and Smith, 2024, new species, is described from Michigan and Minnesota. The recognition of this new species is based on a phylogeny estimated from mitochondrial cytochrome oxidase I and wingless DNA sequences of 25 specimens representing all Paranthrene Hübner species including individuals from various locations. Paranthrene sogaardi new species was monophyletic and differed from Paranthrene tabaniformis Rottenburg, 1775 by a mean of 7.6% COI pairwise “p” distance, coloration, and genitalic morphology.

ZooBank registration. urn:lsid:zoobank.org:pub:8B9ED6CE-4DEB-4CB2-9235-3CA593F4D9DB

Corrections And Additions To The Hydnocerina (Coleoptera: Cleridae: Clerinae: Hydnocerini) Of Mexico, John M. Leavengood Jr.

Insecta Mundi

Corrections, additions and remarks are made with respect to the recent checklist of the Cleridae (Coleoptera) of Mexico (Araujo-Castillo et al. 2024). Current valid names, synonymies, clarifications of taxonomic problems, and omitted taxa are reviewed. This work is intended to serve as a supplement to the “Hydnocerinae” section of the checklist.

ZooBank registration. urn:lsid:zoobank.org:pub:EF3E45B5-6EA6-41AB-B92F-DB4ABF9D8215

Taxonomic Notes On Velvet Ants Of The Genus Timulla Ashmead, 1899 (Hymenoptera: Mutillidae) In The United States Of America And Canada, George C. Waldren

Insecta Mundi

The exclusively New World velvet ant genus Timulla Ashmead, 1899 (Hymenoptera: Mutillidae: Mutillinae: Trogaspidiini) contains 180 species and ten subspecies. Most of these species are known from a single sex, and the validity of the subspecies has not been evaluated since their original description in 1937 and 1938. The Timulla fauna of the United States of America includes thirty species and eight subspecies, and the fauna of Canada includes three species. The faunas of these two countries were critically studied with the following results. Out of the eight total subspecies, seven were found to be structurally identical to and sympatric …

Long Time No See: New Reports Of Legua Rosea Amédégnato And Poulain, 1986 (Orthoptera: Caelifera: Romaleidae: Romaleinae: Leguini), A Rare Arboreal Grasshopper From Brazil, Daniela Santos Martins Silva, Renan Da Silva Olivier, Larissa Lima De Queiroz, Marcelo Ribeiro Pereira, Jardel Boscardin, Guilherme Rabelo D’Angelis

Insecta Mundi

The genus Legua Walker, 1870 comprises only two known species from Central America and Brazil, with a notable scarcity of collected specimens. Herein, we provide a new contribution to our knowledge of the distribution of Legua rosea Amédégnato and Poulain, 1986 (Romaleinae: Leguini), that has a restricted distribution in Brazil, based on entomological collection data and social media information. Our records emphasize the importance of natural history collections and new tools for biodiversity studies.

ZooBank registration. urn:lsid:zoobank.org:pub:17E0802B-A389-4983-AC74-21B51197F557

Internet-Based Data Platforms Re-Define The Distributions Of Some Large Crabronid Wasps In Arkansas (Hymenoptera: Crabronidae), David E. Bowles

Insecta Mundi

The geographic distributions of three large wasps, Sphecius speciosus (Drury), Stictia carolina Fabricius, and Stizus brevipennis Walsh (Hymenoptera: Crabronidae), occurring in Arkansas are defined using museum specimens and three internet-based data platforms. The internet-based data platforms generally provided more county location records than museum records. Using data from internet sources for easily identified species can better serve to illustrate the known distributions for some species thus making for a powerful tool elucidating distributional patterns and conservation planning.

ZooBank registration. urn:lsid:zoobank.org:pub:DCAE9192-1765-40CD-952B-0A094F413991

Nonlinear Classifiers For Wet-Neuromorphic Computing Using Gene Regulatory Neural Network, Adrian Ratwatte, Samitha Somathilaka, Sasitharan Balasubramaniam, Assaf A. Gilad

School of Computing: Faculty Publications

The gene regulatory network (GRN) of biological cells governs a number of key functionalities that enable them to adapt and survive through different environmental conditions. Close observation of the GRN shows that the structure and operational principles resemble an artificial neural network (ANN), which can pave the way for the development of wet-neuromorphic computing systems. Genes are integrated into gene-perceptrons with transcription factors (TFs) as input, where the TF concentration relative to half-maximal RNA concentration and gene product copy number influences transcription and translation via weighted multiplication before undergoing a nonlinear activation function. This process yields protein concentration as the …

Plas 439: Organic Farming And Food Systems Faculty-Led Inquiry Into Reflective Scholarly Teaching Benchmark Portfolio, Christian Stephenson

UNL Faculty Course Portfolios

Organic Farming and Food Systems is a senior and graduate level course for students in the Department of Agronomy and Horticulture. This course was previously offered but has been significantly modified as I have taken on responsibility for the course. Goals for the course include student comprehension of the methods of organic and regenerative farming and the impacts of those methods on economic, environmental, and social sustainability. An additional goal is to build student competency in the evaluation of primary, secondary, and tertiary information resources and critical thinking surrounding issues in food production. Assessment for the course was through diverse …