Digital Commons Network^™

Opda Regulates Maize Defense Against Aphids, Suresh Varsani, Sajjan Grover, Shaoqun Zhou, Kyle G. Koch, Pei-Cheng Huang, Michael V. Kolomiets, W. Paul Williams, Tiffany Heng-Moss, Gautam Sarath, Dawn S. Luthe, Georg Jander, Joe Louis

Department of Entomology: Faculty Publications

Copyright © 2019 American Society of Plant Biologists.

DOI:10.1104/pp.18.01472

Rnai Targeting Of Rootworm Troponin I Transcripts Confers Root Protection In Maize, Elane Fishilevich, Andrew J. Bowling, Meghan L. F. Frey, Po-Hao Wang, Wendy Lo, Murugesan Rangasamy, Sarah E. Worden, Heather E. Pence, Premchand Gandra, Shannon L. Whitlock, Greg Schulenberg, Eileen Knorr, Linda Tenbusch, Jamie R. Lutz, Stephen Novak, Ronda L. Hamm, Karl D. Schnelle, Andreas Vilcinskas, Kenneth E. Narva

Department of Entomology: Faculty Publications

Western corn rootworm, Diabrotica virgifera virgifera, is the major agronomically important pest of maize in the US Corn Belt. To augment the repertoire of the available dsRNA-based traits that control rootworm, we explored a potentially haplolethal gene target, wings up A (wupA), which encodes Troponin I. Troponin I, a component of the Troponin-Tropomyosin complex, is an inhibitory protein involved in muscle contraction. In situ hybridization showed that feeding on wupA-targeted dsRNAs caused systemic transcript knockdown in D. v. virgifera larvae. The knockdown of wupA transcript, and by extension Troponin I protein, led to deterioration of the …

P7 And P8 Proteins Of High Plains Wheat Mosaic Virus, A Negative-Strand Rna Virus, Employ Distinct Mechanisms Of Rna Silencing Suppression, Adarsh K. Gupta, Gary L. Hein, Satyanarayana Tatineni

Department of Entomology: Faculty Publications

High Plains wheat mosaic virus (genus Emaravirus), an octapartite negative-sense RNA virus, encodes two RNA silencing suppressors, P7 and P8. In this study, we found that P7 and P8 efficiently delayed the onset of dsRNA-induced transitive pathway of RNA silencing. Electrophoretic mobility shift assays (EMSA) revealed that only P7 protected long dsRNAs from dicing in vitro and bound weakly to 21- and 24-nt PTGS-like ds-siRNAs. In contrast, P8 bound strongly and relatively weakly to 21- and 24-nt ds-siRNAs, respectively, suggesting size-specific binding. In EMSA, neither protein bound to 180-nt and 21-nt ssRNAs at detectable levels. Sequence analysis revealed that …

Do People Really Become More Conservative As They Age?, Johnathan C. Peterson, Kevin Smith, John Hibbing

Department of Political Science: Faculty Publications

Folk wisdom has long held that people become more politically conservative as they grow older, although several empirical studies suggest political attitudes are stable across time. Using data from the Michigan Youth-Parent So- cialization Panel Study, we analyze attitudinal change over a major portion of the adult life span. We document changes in party identification, self-reported ideology, and selected issue positions over this time period and place these changes in context by comparing them with contemporaneous national averages. Consistent with previous research but con- trary to folk wisdom, our results indicate that political attitudes are remarkably stable over the long …

Effects Of Urea And Distillers Inclusion In Finishing Diets On Steer Performance And Carcass Characteristics, Bradley M. Boyd, James C. Macdonald, Matthew K. Luebbe, Galen E. Erickson

Nebraska Beef Cattle Reports

An experiment was conducted to evaluate the effect of supplemental urea in dry rolled corn based finishing diets containing low inclusions of distillers grains. Treatments were set up in a 3 × 3 factorial arrangement. The first factor was wet distillers inclusion at either 10, 15, or 20% of diet DM. The second factor was urea inclusion at either 0, 0.5, or 1.0% of diet dry matter. Increasing inclusion of distillers linearly improved feed conversion and linearly reduced dry matter intake. An interaction for feed efficiency was observed where there was no effect of added urea when 10% or 20% …

Beyond Bird Feed: Proso Millet For Human Health And Environment, Saurav Das, Rituraj Khound, Meenakshi Santra, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated in 8000–10,000 BP in northern China, proso millet (Panicum miliaceum L.) is the best adaptive rotational crop for semiarid central High Plains of the USA, where average annual precipitation is 356–407 mm. Proso millet has multiple benefits when consumed as human food. Proso millet is rich in minerals, dietary fiber, polyphenols, vitamins and proteins. It is gluten-free and therefore, ideal for the gluten intolerant people. Proso millet contains high lecithin which supports the neural health system. It is rich in vitamins (niacin, B-complex vitamins, folic acid), minerals (P, Ca, Zn, Fe) and essential amino acids (methionine and cysteine). …

Winter Wheat Grain Yield Response To Fungicide Application Is Influenced By Cultivar And Rainfall, Emmanuel Byamukama, Shaukat Ali, Jonathan Kleinjan, Dalitso N. Yabwalo, Christopher Graham, Melanie Caffe-Treml, Nathan D. Mueller, John Rickertsen, William A. Berzonsky

Department of Agronomy and Horticulture: Faculty Publications

Winter wheat is susceptible to several fungal pathogens throughout the growing season and foliar fungicide application is one of the strategies used in the management of fungal diseases in winter wheat. However, for fungicides to be profitable, weather conditions conducive to fungal disease development should be present. To determine if winter wheat yield response to fungicide application at the flowering growth stage (Feekes 10.5.1) was related to the growing season precipitation, grain yield from fungicide treated plots was compared to non-treated plots for 19 to 30 hard red winter wheat cultivars planted at 8 site years from 2011 through 2015. …

A Two-Amino Acid Difference In The Coat Protein Of Satellite Panicum Mosaic Virus Isolates Is Responsible For Differential Synergistic Interactions With Panicum Mosaic Virus, R. V. Chowda-Reddy, Nathan A. Palmer, Serge Edme, Gautam Sarath, Frank A. Kovacs, Gary Y. Yuen, Robert Mitchell, Satyanarayana Tatineni

Department of Agronomy and Horticulture: Faculty Publications

Panicum mosaic virus (PMV) (genus Panicovirus, family Tombusviridae) and its molecular parasite, Satellite panicum mosaic virus (SPMV), synergistically interact in coinfected proso and pearl millet (Panicum miliaceum L.) plants resulting in a severe symptom phenotype. In this study, we examined synergistic interactions between the isolates of PMV and SPMV by using PMV-NE, PMV85, SPMV-KS, and SPMV-Type as interacting partner viruses in different combinations. Coinfection of proso millet plants by PMV-NE and SPMV-KS elicited severe mosaic, chlorosis, stunting, and eventual plant death compared with moderate mosaic, chlorotic streaks, and stunting by PMV85 and SPMV-Type. In reciprocal combinations, coinfection …

Soil Fauna Accelerate Dung Pat Decomposition And Nutrient Cycling Into Grassland Soil, Kenneth S. Evans, Martha Mamo, Ana Wingeyer, Walter Schacht, Kent Eskridge, Jeff Bradshaw, Daniel Ginting

Department of Agronomy and Horticulture: Faculty Publications

Soil fauna play critical roles in various ecosystem functions and services, but empirical data measuring their impact on dung pat decomposition and subsequent nutrient cycling into rangeland soils are limited. The objective of this study was to quantify the effect of soil fauna, using dung beetle as an indicator, on dung decomposition and subsequent translocation of dung nutrients into grassland soil over time. A field experiment was conducted early in the summer season and late in the summer season of 2014 and 2015. In each season, dung beetle abundance, changes in dung properties, and subsequent translocation of dung nutrients into …

A Spatial Framework For Ex-Ante Impact Assessment Of Agricultural Technologies, José F. Andrade, Juan I. Rattalino Edreira, Andrew Farrow, Marloes P. Van Loon, Peter Q. Craufurd, Jairos Rurinda, Shamie Zingore, Jordan Chamberlin, Lieven Claessens, Julius Adewopo, Martin K. Van Ittersum, Kenneth G. Cassman, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Traditional agricultural research and extension relies on replicated field experiments, on-farm trials, and demonstration plots to evaluate and adapt agronomic technologies that aim to increase productivity, reduce risk, and protect the environment for a given biophysical and socio-economic context. To date, these efforts lack a generic and robust spatial framework for ex-ante assessment that: (i) provides strategic insight to guide decisions about the number and location of testing sites, (ii) define the target domain for scaling-out a given technology or technology package, and (iii) estimate potential impact from widespread adoption of the technology(ies) being evaluated. In this study, we developed …

Changes On Movement Control Of Dart Throwing Under Distance And Target Weight Constraints, Carlos E. Campos, Guilherme M. Lage, Andre G.P. Andrade, Crislaine R. Couto, Suziane P. Santos, Vitor L.S. Profeta, Herbert Ugrinowitsch

Athletic Performance Research

The aim of the study was to verify the effects of dart weight and target distance on kinematic variables of the movement control of the underarm dart throwing task. Four women and one man performed the task of throwing a dart at two horizontal circular targets located at 2m (Nt) and 3m (Ft) away, with two different weights, 22g (Ld) and 44g (Hd). On the first phase of the experiment, the human volunteers performed 200 trials per day during four sessions. On the fifth day, it had 40 more trials in a pseudo random order that were recorded and analysed. …

Actual Evapotranspiration And Crop Coefficients Of Irrigated Lowland Rice (Oryza Sativa L.) Under Semiarid Climate, Koffi Djaman, Daran R. Rudnick, Yonnelle D. Moukoumbi, Abdoulaye Sow, Suat Irmak

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Lowland irrigated rice is the predominant crop produced in the Senegal River Valley characterized by very low annual rainfall, high temperatures, and low relative humidity. The Senegal River is shared by Senegal, Mali, Mauritania, and Guinea, and serves as the main source of irrigation water for the adopted double rice cropping system. Developing appropriate resource management strategies might be the key factor for the sustainability of rice production in the region. This study aims to estimate rice seasonal evapotranspiration (ETa), irrigation water requirement, and to develop rice growth stage specific crop coefficients (Kc) to improve rice water productivity. Field experiments …

Metabolic Compounds Within The Porcine Uterine Environment Are Unique To The Type Of Conceptus Present During The Early Stages Of Blastocyst Elongation, Sophie C. Walsh, Jeremy R. Miles, Linxing Yao, Corey D. Broeckling, Lea A. Rempel, Elane C. Wright-Johnson, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

The objective of this study was to identify metabolites within the porcine uterine milieu during the early stages of blastocyst elongation. At Days 9, 10, or 11 of gestation, reproductive tracts of White cross‐bred gilts (n = 38) were collected immediately following harvest and flushed with Roswell Park Memorial Institute‐1640 medium. Conceptus morphologies were assessed from each pregnancy and corresponding uterine flushings were assigned to one of five treatment groups based on these morphologies: (a) uniform spherical (n = 8); (b) heterogeneous spherical and ovoid (n = 8); (c) uniform ovoid (n = 8); (d) heterogeneous …

Interactions Between Inflammation And Female Sexual Desire And Arousal Function, Tierney K. Lorenz

Department of Psychology: Faculty Publications

Purpose of Review—To describe the current state of research on interactions between inflammation and female sexual function.

Recent findings—Inflammation may interfere with female sexual desire and arousal via direct (neural) and indirect (endocrine, vascular, social/behavioral) pathways. There are significant sex differences in the effect of inflammation on sexual function, arising from different evolutionary selection pressures on regulation of reproduction. A variety of inflammation-related conditions are associated with risk of female sexual dysfunction, including cardiovascular disease, metabolic syndrome, and chronic pain.

Summary—Clinical implications include the need for routine assessment for sexual dysfunction in patients with inflammation-related conditions, the potential for anti-inflammatory …

Verbal Descriptions Of Cue Direction Affect Object Desirability, Jason Tipples, Mike Dodd, Jordan Grubaugh, Alan Kingstone

Department of Psychology: Faculty Publications

Approach-avoidance behaviors are observed across a broad range of species. For humans, we tend move toward things we like, and away from things we dislike. Previous research tested whether repeatedly shifting visuo-spatial attention toward an object in response to eye gaze cues can increase liking for that object. Here, we tested whether a gaze-liking effect can occur for verbal descriptions of looking behavior without shifts of attention. Also, we tested the gaze specificity hypothesis – that the liking effect is specific to gaze cues – by comparing the effect of different types of cue (pointing gestures and arrow cues). In …

Biological Risk Profiles Among Latino Subgroups In The Health And Retirement Study, Catherine García, Jennifer A. Ailshire

Department of Sociology: Faculty Publications

Background and Objectives: Latinos residing in the United States exhibit an increased risk for cardiovascular and meta- bolic diseases compared to non-Latino whites. This elevated risk contributes to a significantly higher prevalence of diabetes and hypertension among Latino adults. Examining biological risk profiles of older Latinos as a “pan-ethnic group” and by Latino subpopulations may help to explain the increased burden of disease in later life among this population. The objec- tive of this study is to document biological risk profiles among a nationally representative sample of older U.S. Latinos by nativity and country of origin.

Research Design and Methods: …

Culture And The Development Of Traditional Medicine In Africa, Rowland Edet, Oyedolapo Isaac Bello, Julianah Babajide

Department of Sociology: Faculty Publications

Traditional medicine has been the dominant healthcare system in Africa before westernization, civilization and colonialism. For people living in the rural areas, traditional medicine is easily available, accessible and affordable. The purpose of this paper is to examine how the cultural way of the life of Africans has contributed to the emergence and development of traditional therapeutic systems in the continent. By explaining the way Africans perceive illness and disease, this paper argues that various forms of healing were predicated on the sociocultural environment of the people. The paper therefore opts for concerted efforts in the development of traditional medicine …

Disability And The Immigrant Health Paradox: Gender And Timing Of Migration, Marc A. Garcia, Adriana M. Reyes, Sunshine Rote

Department of Sociology: Faculty Publications

Although research has documented better health and longer life expectancy among the foreign-born relative to their U.S.-born counterparts, the U.S. Mexican-origin immigrant population is diverse and the healthy immigrant effect likely varies by key structural and demographic factors such as gender, migration history, and duration in the United States. Using a life course framework, we use data from the Hispanic Established Populations for the Epidemiologic Study of the Elderly (H-EPESE 1993–2013) which includes Mexican-American individuals aged 65 and older to assess the heterogeneity in the immigrant health advantage by age of migration and gender. We find that age of migration …

Late Life Depressive Symptoms And Cognitive Function Among Older Mexican Adults: The Past And The Present, Joseph L. Saenz, Marc A. Garcia, Brian Downer

Department of Sociology: Faculty Publications

Objective: To evaluate associations between depression and individual cognitive domains and how changes in depressive symptoms relate to cognition three years later in the context of Mexico, a developing country experiencing rapid aging.

Method: Data comes from waves 3 (2012) and 4 (2015) of the Mexican Health and Aging Study (n=12,898, age 50+). Depression is ascertained using a modified Center for Epidemiologic Studies – Depression Scale. Cognition is assessed using verbal learning, verbal memory, visual scanning, verbal fluency, visuospatial ability, visual memory, and orientation tasks. Depressive symptoms and cognitive functioning were both measured in 2012 and 2015. Scores across cognitive …

When Viruses Infect Plants, Hernan Garcia-Ruiz

Department of Plant Pathology: Faculty Publications

Just as human beings can catch a cold, plants can also get viral infections. Understanding the mechanisms regulating the interactions between plants and viruses is the first step towards developing better management strategies and using biotechnology methods to immunise plants and engineer genetic resistance to viruses in plants. This is the focus of research by Dr Hernan Garcia-Ruiz and his team based at the University of Nebraska, USA.

Viral diseases in plants can cause important economic losses as a result of poor-quality products and lower yield. This impact can particularly seriously affect developing countries which are more likely to be …

Host Factors Against Plant Viruses, Hernan García-Ruiz

Department of Plant Pathology: Faculty Publications

Plant virus genome replication and movement is dependent on host resources and factors. However, plants respond to virus infection through several mechanisms, such as autophagy, ubiquitination, mRNA decay and gene silencing, that target viral components. Viral factors work in synchrony with pro-viral host factors during the infection cycle and are targeted by antiviral responses. Accordingly, establishment of virus infection is genetically determined by the availability of the pro-viral factors necessary for genome replication and movement, and by the balance between plant defence and viral suppression of defence responses. Sequential requirement of pro-viral factors and the antagonistic activity of antiviral factors …

Allowable Take Of Black Vultures In The Eastern United States, Guthrie Zimmerman, Brian A. Millsap, Michael L. Avery, John R. Sauer, Michael C. Runge, Kenneth D. Richkus

United States Department of Agriculture Wildlife Services: Staff Publications

Black vultures (Coragyps atratus) have been increasing in density and expanding their range in the eastern United States since at least the 1960s. In many areas, their densities have increased to the level where they are causing damage to property and livestock and the number of requests for allowable take permits has increased throughout these areas. The United States Fish and Wildlife Service (USFWS) requires updated information to help inform the number of take permits that could reduce conflicts while meeting obligations under the Migratory Bird Treaty Act.We expanded analyses used to estimate allowable take in Virginia to …

Effects Of Repeated Sublethal External Exposure To Deep Water Horizon Oil On The Avian Metabolome, Brian S. Dorr, Katie C. Hanson-Dorr, Fariba M. Assadi-Porter, Ebru Selin Selen, Katherine A. Healy, Katherine E. Horak

United States Department of Agriculture Wildlife Services: Staff Publications

We assessed adverse effects of external sublethal exposure of Deepwater Horizon, Mississippi Canyon 252 oil on plasma and liver metabolome profiles of the double-crested cormorant (Phalacrocorax auritus), a large (1.5 to 3.0 kg) diving waterbird common in the Gulf of Mexico. Metabolomics analysis of avian plasma showed significant negative effects on avian metabolic profiles, in some cases after only two external exposures (26 g cumulative) to oil. We observed significant (p < 0.05) changes in intermediate metabolites of energy metabolism and fatty acid and amino acid metabolic pathways in cormorants after repeated exposure to oil. Exposure to oil increased several metabolites (glycine, betaine, serine and methionine) that are essential to the one-carbon metabolism pathway. Lipid metabolism was affected, causing an increase in production of ketone bodies, suggesting lipids were used as an alternative energy source for energy production in oil exposed birds. In addition, metabolites associated with hepatic bile acid metabolism were affected by oil exposure which was correlated with changes observed in bile acids in exposed birds. These changes at the most basic level of phenotypic expression caused by sublethal exposure to oil can have effects that would be detrimental to reproduction, migration, and survival in avian species.

The Conundrum Of Agenda-Driven Science In Conservation, M. Zachariah Peery, Gavin M. Jones, R. J. Gutierrez, Steve M. Redpath, Alan B. Franklin, Daniel Simberloff, Monica G. Turner, Volker C. Radeloff, Gary C. White

United States Department of Agriculture Wildlife Services: Staff Publications

Conservation biology is a value-laden discipline predicated on conserving biodiversity (Soulé 1985), a mission that does not always sit easily with objective science (Lackey 2007; Pielke 2007; Scott et al. 2007). While some encourage scientists to be responsible advocates for conservation (Garrard et al. 2016), others worry that objectivity in conservation research may suffer (Lackey 2007). At this time, we believe advocacy by scientists is essential for environmental conservation and, indeed, humanity. It is difficult to envision the state of our environment had scientists failed to encourage policy makers and the public to address emerging conservation problems. Nevertheless, conservation scientists …

Group Effects Of A Non-Native Plant Invasion On Rodent Abundance, Bryan M. Kluever, Trinity N. Smith, Eric M. Gese

United States Department of Agriculture Wildlife Services: Staff Publications

Cheatgrass (Bromus tectorum) is the most prolific invading plant in western North America. Investigations determining the impact of this invasion on population state variables and community dynamics of rodents have largely occurred at the community or species level, creating a knowledge gap as to whether rodents affiliated by a shared taxonomy or other grouping are differentially affected by cheatgrass invasion. We examined rodent abundance along a gradient of cheatgrass cover using various groupings of two nocturnal rodent taxa comprising the majority of the rodent community in the Great Basin Desert. In the summers of 2010–2013, rodents were sampled …

Machine Learning To Classify Animal Species In Camera Trap Images: Applications In Ecology, Michael A. Tabak, Mohammad S. Norouzzadeh, David W. Wolfson, Steven J. Sweeney, Kurt C. Vercauteren, Nathan P. Snow, Joseph M. Halseth, Paul A. Di Salvo, Jesse S. Lewis, Michael D. White, Ben Teton, James C. Beasley, Peter E. Schlichting, Raoul K. Boughton, Bethany Wight, Eric S. Newkirk, Jacob S. Ivan, Eric A. Odell, Ryan K. Brook, Paul M. Lukacs, Anna K. Moeller, Elizabeth G. Mandeville, Jeff Clune, Ryan S. Miller

United States Department of Agriculture Wildlife Services: Staff Publications

1. Motion-activated cameras (“camera traps”) are increasingly used in ecological and management studies for remotely observing wildlife and are amongst the most powerful tools for wildlife research. However, studies involving camera traps result in millions of images that need to be analysed, typically by visually observing each image, in order to extract data that can be used in ecological analyses.

2. We trained machine learning models using convolutional neural networks with the ResNet-18 architecture and 3,367,383 images to automatically classify wildlife species from camera trap images obtained from five states across the United States. We tested our model on an …

Cause‐Specific Mortality Of The World’S Terrestrial Vertebrates, Jacob E. Hill, Travis L. Devault, Jerrold L. Belant

United States Department of Agriculture Wildlife Services: Staff Publications

Aim: Vertebrates are declining worldwide, yet a comprehensive examination of the sources of mortality is lacking. We conducted a global synthesis of terrestrial vertebrate cause‐specific mortality to compare the sources of mortality across taxa and determine predictors of susceptibility to these sources of mortality.

Location: Worldwide.

Time period: 1970–2018.

Major taxa studied: Mammals, birds, reptiles and amphibians.

Methods: We searched for studies that used telemetry to determine the cause of death of terrestrial vertebrates. We determined whether each mortality was caused by anthropogenic or natural sources and further classified mortalities within these two categories (e.g. harvest, vehicle collision and predation). …

Feral Swine Harming Insular Sea Turtle Reproduction: The Origin, Impacts, Behavior And Elimination Of An Invasive Species, Richard M. Engeman, Robert W. Byrd, Jamie Dozier, Mark A. Mcalister, James O. Edens, Elizabeth M. Kierepka, Timothy J. Smyser, Noel Myers

United States Department of Agriculture Wildlife Services: Staff Publications

Feral swine are among the world's most destructive invasive species wherever they are found, with translocations figuring prominently in their range expansions. In contrast, sea turtles are beloved species that are listed as threatened or endangered throughout the world and are the focus of intense conservation efforts. Nest predation by feral swine severely harms sea turtle reproduction in many locations around the world. Here we quantify and economically assess feral swine nest predation at North Island, South Carolina, an important loggerhead sea turtle nesting beach. Feral swine depredation of North Island sea turtle nests was first detected in 2005, with …

A Bioeconomic Model For The Optimization Of Local Canine Rabies Control, Aaron Anderson, Johann Kotze, Stephanie A. Shwiff, Brody Hatch, Chris Slootmaker, Anne Conan, Darryn Knobel, Louis H. Nel

United States Department of Agriculture Wildlife Services: Staff Publications

We present a new modeling tool that can be used to maximize the impact of canine rabies management resources that are available at the local level. The model is accessible through a web-based interface that allows for flexibility in the management strategies that can be investigated. Rabies vaccination, sterilization, chemo-contraception, and euthanasia can be specified and limited to specific demographic groups. Additionally, we allowed for considerable complexity in the specification of management costs. In many areas, the costs of contacting additional dogs increases as management effort increases, and this can have important strategic implications. We illustrated the application of the …

No Detection Of Brodifacoum Residues In The Marine And Terrestrial Food Web Three Years After Rat Eradication At Palmyra Atoll, Central Pacific, A. Wegmann, G. Howald, S. Kropidlowski, N. Holmes, A.B. Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Invasive alien species represent one of the greatest threats to native plants and animals on islands. Rats (Rattus spp.) have invaded most of the world’s oceanic islands, causing lasting or irreversible damage to ecosystems and biodiversity. To counter this threat, techniques to eradicate invasive rats from islands have been developed and applied across the globe. Eradication of alien rats from large or complex island ecosystems has only been successful with the use of bait containing a rodenticide. While effective at eradicating rats from islands, rodenticide can persist in the ecosystem longer than the time required to eradicate the target rat …