Digital Commons Network^™

Association Of Age, Sex, And Pyrethroid Resistance Status On Survival And Cytochrome P450 Gene Expression In Aedes Aegypti (L.), Leslie C. Rault, Scott T. O'Neal, Ellis J. Johnson, Troy D. Anderson

Department of Entomology: Faculty Publications

Aedes aegypti is a vector of viruses that negatively impact human health. Insecticide resistance complicates mosquito control efforts, but understanding the mechanisms of resistance can help to improve management practices. This study examined different factors that could influence the interpretation of toxicity bioassays and gene expression studies in A. aegypti, including sex and age, in the context of resistance to pyrethroids. Bioassays using a pyrethroid-resistant strain, Puerto Rico (PR), and a pyrethroid-susceptible strain, Rockefeller (Rock), of A. aegypti were conducted with females and males of three age groups to determine differences in mortality induced by deltamethrin. Overall, strain was …

Southern Corn Rootworm (Coleoptera: Chrysomelidae) Adult Emergence And Population Growth Assessment After Selection With Vacuolar Atpase-A Double-Stranded Rna Over Multiple Generations, Adriano E. Pereira, Brigitte Tenhumberg, Lance J. Meinke, Blair D. Siegfried

Department of Entomology: Faculty Publications

The southern corn rootworm, Diabrotica undecimpunctata howardi Barber (Coleoptera: Chrysomelidae), was exposed over multiple generations to vacuolar (v)ATPase-A double-stranded (ds)RNA, first as adults and later, as neonate larvae. During adult selection, high mortality and lower fecundity were observed in the RNAi-selected cages after beetles were exposed to sublethal dsRNA concentrations that varied between LC40 and LC75. During larval selection, a delay in adult emergence and effects on population growth parameters were observed after neonates were exposed to sublethal dsRNA concentrations that varied between LC50 and LC70. Some of the parameters measured for adult emergence such as time to reach maximum …

Metabolomics Of Sorghum Roots During Nitrogen Stress Reveals Compromised Metabolic Capacity For Salicylic Acid Biosynthesis, Amy M. Sheflin, Dawn Chiniquy, Chaohui Yuan, Emily Goren, Indrajit Kumar, Max Braud, Thomas Brutnell, Andrea L. Eveland, Susannah Tringe, Peng Liu, Stephen Kresovich, Ellen Marsh, Daniel P. Schachtman, Jessica E. Prenni

Nebraska Center for Biotechnology: Faculty and Staff Publications

Sorghum (Sorghum bicolor [L.] Moench) is the fifth most productive cereal crop worldwide with some hybrids having high biomass yield traits making it promising for sustainable, economical biofuel production. To maximize biofuel feedstock yields, a more complete understanding of metabolic responses to low nitrogen (N) will be useful for incorporation in crop improvement efforts. In this study, 10 diverse sorghum entries (including inbreds and hybrids) were field-grown under low and full N conditions and roots were sampled at two time points for metabolomics and 16S amplicon sequencing. Roots of plants grown under low N showed altered metabolic profiles at …

Comparison Of Different Functions To Describe Growth From Weaning To Maturity In Crossbred Beef Cattle, Madeline J. Zimmermann, Larry A. Kuehn, Matthew L. Spangler, R. Mark Thallman, Warren M. Snelling, Ronald M. Lewis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Cow mature weight (MWT) has increased in the past 30 yr. Larger cows cost more to maintain, but their efficiency—and thus profitability— depends on the production environment. Incorporating MWT effectively into selection and mating decisions requires understanding of growth to maturity. The objective of this study was to describe growth to maturity in crossbred beef cattle using Brody, spline, and quadratic functions. Parameter estimates utilized data on crossbred cows from cycle VII and continuous sampling phases of the Germplasm Evaluation Program at the U.S. Meat Animal Research Center. The MWT were estimated at 6 yr from the fitted parameters obtained …

Spatio-Temporal Patterns And Characteristics Of Swine Shipments In The U.S. Based On Interstate Certificates Of Veterinary Inspection, Erin E. Gorsich, Ryan S. Miller, Holly M. Mask, Clayton Hallman, Katie Portacci, Colleen T. Webb

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Domestic swine production in the United States is a critical economic and food security industry, yet there is currently no large-scale quantitative assessment of swine shipments available to support risk assessments. In this study, we provide a national-level characterization of the swine industry by quantifying the demographic (i.e. age, sex) patterns, spatio-temporal patterns, and the production diversity within swine shipments. We characterize annual networks of swine shipments using a 30% stratified sample of Interstate Certificates of Veterinary Inspection (ICVI), which are required for the interstate movement of agricultural animals. We used ICVIs in 2010 and 2011 from eight states that …

Partners In School: An Innovative Parent-Teacher Consultation Model For Children With Autism Spectrum Disorder, Gazi F. Azad, Steven C. Marcus, Susan M. Sheridan, David S. Mandell

Department of Educational Psychology: Faculty Publications

Little research examines the best ways to improve communication between parents and teachers of children with Autism Spectrum Disorder (ASD) and its effect on child outcomes. The present study tests an innovative parent-teacher consultation model, entitled Partners in School. The goal of Partners in School is to improve parent-teacher communication about evidence-based practices (EBPs), and subsequently, outcomes for children with ASD. Participants were 26 teachers and 49 parents of children with ASD from a large urban public school district. Parents and teachers completed measures of their communication and child outcomes prior to and after receiving consultation through Partners in School. …

Functional Random Forest With Applications In Dose-Response Predictions, Raziur Rahman, Saugato Rahman Dhruba, Souparno Ghosh, Ranadip Pal

Department of Statistics: Faculty Publications

Drug sensitivity prediction for individual tumors is a significant challenge in personalized medicine. Current modeling approaches consider prediction of a single metric of the drug response curve such as AUC or IC₅₀. However, the single summary metric of a dose-response curve fails to provide the entire drug sensitivity profile which can be used to design the optimal dose for a patient. In this article, we assess the problem of predicting the complete dose-response curve based on genetic characterizations. We propose an enhancement to the popular ensemble-based Random Forests approach that can directly predict the entire functional profile of …

Autodissemination Of Pyriproxyfen As A Method For Controlling The House Fly Musca Domestica, Haim Biale, Elad Chiel, Christopher J. Geden

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

House fly (Musca domestica) control is a major challenge in animal agriculture. Here, we tested the feasibility of applying pyriproxyfen (PPF), an insect-growth regulator that controls house flies effectively, using autodissemination methods, in which the flies themselves deliver PPF to their oviposition sites. First, we tried baiting gravid female flies to walk-through stations, where flies would self-treat with PPF and distribute it. This concept worked well in laboratory and indoor cage experiments, but not in the field, as flies appeared reluctant to alight on and collect PPF. Therefore, we tested a different concept of actively coating flies with PPF and …

Micrornas In Bovine Milk Exosomes Are Bioavailable In Humans But Do Not Elicit A Robust Pro-Inflammatory Cytokine Response, Ezra Mutai, Amanda E. Ramer-Tait, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Background: Bovine milk exosomes are studied for their roles as bioactive food compounds and as vehicles for drug delivery. Both lines of investigation converge on immune function, e.g., immune regulation by absorption of microRNAs encapsulated in milk exosomes across species boundaries, and the possibility of exosomes and their cargos triggering an immune response if used in drug delivery. This study assessed the bioavailability of immune-related microRNAs from bovine milk and changes in plasma cytokine concentrations after milk consumption in humans, and the secretion of cytokines by human peripheral blood mononuclear cells (PBMCs) cultured with milk exosomes transfected with immune-relevant microRNAs. …

Environmental And Agricultural Relevance Of Humic Fractions Extracted By Alkali From Soils And Natural Waters, D. C. Olk, P. R. Bloom, E. M. Perdue, D. M. Mcknight, Y. Chen, A. Farenhorst, N. Senesi, Y.-P Chin, P. Schmitt-Kopplin, N. Hertkorn, M. Harir

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

To study the structure and function of soil organic matter, soil scientists have performed alkali extractions for soil humic acid (HA) and fulvic acid (FA) fractions for more than 200 years. Over the last few decades aquatic scientists have used similar fractions of dissolved organic matter, extracted by resin adsorption followed by alkali desorption. Critics have claimed that alkaliextractable fractions are laboratory artifacts, hence unsuitable for studying natural organic matter structure and function in field conditions. In response, this review first addresses specific conceptual concerns about humic fractions. Then we discuss several case studies in which HA and FA were …

Blood Parasites Infecting The Hoatzin (Opisthocomus Hoazin), A Unique Neotropical Folivorous Bird, M. Andreína Pacheco, M. Alexandra García-Amado, Jaime Manzano, Nubia E. Matta, Ananias A. Escalante

Harold W. Manter Laboratory of Parasitology: Library Materials

The Hoatzin (Opisthocomus hoazin) is the only extant member of the order Opisthocomiformes. This unique South American bird lives in the riparian lowland vegetation characteristic of the Amazon and Orinoco basins. Hoatzins nest in communal social units close to water bodies; they are strictly folivores being the only bird with pregastric fermentation in the crop. Because of the complex logistics involved in capturing this bird, there is a knowledge gap on its parasites. This study documents two distant lineages of haemosporidian parasites (Plasmodium spp.) in a juvenile and two adults sampled in the Cojedes state, Venezuela. Although …

Electromagnon Excitation In Cupric Oxide Measured By Fabry-Pérot Enhanced Terahertz Mueller Matrix Ellipsometry, Sean Knight, Dharmalingam Prabhakaran, Christian Binek, Mathias Schubert

Christian Binek Publications

Here we present the use of Fabry-Pérot enhanced terahertz (THz) Mueller matrix ellipsometry to measure an electromagnon excitation in monoclinic cupric oxide (CuO). As a magnetically induced ferroelectric multiferroic, CuO exhibits coupling between electric and magnetic order. This gives rise to special quasiparticle excitations at THz frequencies called electromagnons. In order to measure the electromagnons in CuO, we exploit single-crystal CuO as a THz Fabry-Pérot cavity to resonantly enhance the excitation’s signature. This enhancement technique enables the complex index of refraction to be extracted. We observe a peak in the absorption coefficient near 0.705 THz and 215 K, which corresponds …

Carbon Dioxide (Co2) And Methane (Ch4) Fluxes From Agricultural Drainage Canals At The Timberlake Observatory For Wetland Restoration In North Carolina’S Coastal Plain, Hannah V. Schleupner, Katherine L. Juarez, Mary J. Carmichael

RURALS: Review of Undergraduate Research in Agricultural and Life Sciences

Wetlands play an integral role in the global biogeochemical cycling of carbon (C). Historically, research on carbon dynamics in wetlands has focused on large-scale wetlands that are easily recognized on the landscape. However, cryptic wetlands- wetlands that may be small-scale, seasonally inundated, and/or otherwise difficult to identify or characterize on a landscape- occur throughout the Earth’s surface. Agricultural drainage canals represent one type of cryptic wetland environment. Even though they are individually small, the collective imprint of drainage canals across a landscape, especially in low-lying regions where land use is dominated by agriculture, may be large. Herein, we measure CO …

Hexapod Herald - Vol. 31, No. 1, February 2019

Hexapod Herald and Other Entomology Department Newsletters

Welcome -- Congratulations -- Note from John Ruberson -- Grants -- Faculty News -- Student News -- Publications -- Blast from the Past -- Brewer Appreciation Reception -- Intro Beekeeping Class-Year 1 -- Condolences -- Retirement Reception for Kalisch and Roselle

Winter Field Report, December 2018 To February 2019, W. Ross Silcock

Nebraska Bird Review

As has been the trend in recent winters, and despite the relative coolness of this winter, there were numerous examples of species north of expected midwinter ranges or present in higher numbers than usual in winter. The long list includes unexpected midwinter records of Tundra Swan, American Wigeon, Ring-necked Duck, Lesser Scaup, White-winged Dove, Sandhill Crane, Clark’s Grebe, Doublecrested Cormorant, Red-shouldered Hawk, and Yellow-bellied Sapsucker. Startling was the state’s first winter record of Sedge Wren, perhaps the biggest rarity of the season. And Eastern Meadowlark was documented in midwinter for the first time, although its presence in the southeast has …

Bird Monitoring At Effigy Mounds National Monument, Iowa, Status Report 2009–2017, David G. Peitz, Lloyd W. Morrison, Kristen L. Mecke

United States National Park Service: Publications

Executive Summary

In 2009, the Heartland Inventory and Monitoring (I&M) Network) initiated breeding bird surveys on Effigy Mounds National Monument (NM), Iowa, to address two objectives: (1) to monitor changes in bird community composition and abundance, and (2) to improve our understanding of relationships between breeding birds and habitat and the effects of management actions on such relationships. This report evaluates trends in the park’s breeding bird populations in the context of trends observed within the North American Bird Conservation Initiative’s (NABCI) Prairie Hardwood Transition Bird Conservation Region, the bird conservation region in which the park is located. By doing …

Evaluation Of Stabilized Fertilizer And Crop Canopy Sensors As Next-Generation Nitrogen Management Technologies In Irrigated Corn, Leonardo Mendes Bastos

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen (N) is often the most limiting nutrient to corn. Once applied to the field, N can be lost through different pathways, which contributes to low N use efficiency (NUE) by plants. Increases in NUE and decreases in N losses can be potentially achieved by using management options that allow a better synchrony between N supply and demand, such as stabilized fertilizers, and spatially-variable sensor-derived in-season N application. Three studies were conducted in order to assess the effects of different stabilized fertilizers and crop canopy sensors on irrigated corn yield. The first study evaluated the effect of urease inhibitor on …

Deficit Irrigation Management Of Maize In The High Plains Aquifer Region: A Review, Daran Rudnick, Sibel Irmak, C. West, J.L. Chavez, I. Kisekka, T.H. Marek, J.P. Schneekloth, D. Mitchell Mccallister, V. Sharma, K. Djaman, J. Aguilar, M.E. Schipanski, D.H. Rogers, A. Schlegel

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Irrigated agriculture is a major economic contributor of the High Plains Region and it primarily relies on the High Plains Aquifer as a source of water. Over time, areas of the High Plains Aquifer have experienced drawdowns limiting its ability to supply sufficient water to sustain fully irrigated crop production. This among other reasons, including variable climatic factors and differences in state water policy, has resulted in some areas adopting and practicing deficit irrigation management. Considerable research has been conducted across the High Plains Aquifer region to identify locally appropriate deficit irrigation strategies. This review summarizes and discusses research conducted …

Children Of Mumbai’S Brothels: Investigating Developmental Prospects, Primary Relationships, And Service Provision, Rochelle L. Dalla, Sarah Erwin, Lee Kreimer

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To understand the context of the lives of children reared in India’s red-light brothel districts. Background: Substantial empirical insight has emerged on the commercial sexual exploitation of children (CSEC). Yet the extant literature on brothel-based children (BBC), a uniquely vulnerable subset of at-risk children, is paradoxically deficient. Understanding the developmental needs of BBC is critical to mitigating risk.

Method: In-depth, semistructured interviews were conducted with 9 service providers and 30 women residing in 2 red-light brothel districts of Mumbai. Phenomenological inquiry informed the research methodology and data analysis.

Results: Mothers’ goals for children included survival, academic success, and future …

Biomaterial Substrate Modifications That Influence Cell-Material Interactions To Prime Cellular Responses To Nonviral Gene Delivery, Amy Mantz, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Gene delivery is the transfer of exogenous genetic material into somatic cells to modify their gene expression, with applications including tissue engineering, regenerative medicine, sensors and diagnostics, and gene therapy. Viral vectors are considered the most effective system to deliver nucleic acids, yet safety concerns and many other disadvantages have resulted in investigations into an alternative option, i.e. nonviral gene delivery. Chemical nonviral gene delivery is typically accomplished by electrostatically complexing cationic lipids or polymers with negatively charged nucleic acids. Unfortunately, nonviral gene delivery suffers from low efficiency due to barriers that impede transfection success, including intracellular processes such as …

Meaningful Learner Information For Mooc Instructors Examined Through A Contextualized Evaluation Framework, Kerrie Douglas, Mitch Zielinski, Hillary Merzdorf, Heidi Diefes-Dux, Peter Bermel

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Improving STEM MOOC evaluation requires an understanding of the current state of STEM MOOC evaluation, as perceived by all stakeholders. To this end, we investigated what kinds of information STEM MOOC instructors currently use to evaluate their courses and what kinds of information they feel would be valuable for that purpose. We conducted semi-structured interviews with 14 faculty members from a variety of fields and research institutions who had taught STEM MOOCs on edX, Coursera, or Udacity. Four major themes emerged related to instructors’ desires: (1) to informally assess learners as an instructor might in a traditional classroom, (2) to …

Foundations For Open Scholarship Strategy Development, Version 2.1 [Pre-Print], Jonathan Tennant, Jennifer E. Beamer, Jeroen Bosman, Björn Brembs, Neo Christopher Chung, Gail Clement, Tom Crick, Jonathan Dugan, Alastair Dunning, David Eccles, Asura Enkhbayar, Daniel Graziotin, Rachel Harding, Johanna Havemann, Daniel S. Katz, Kshitiz Khanal, Jesper Norgaard Kjaer, Tim Koder, Paul Macklin, Christopher R. Madan, Paola Masuzzo, Lisa Matthias, Katja Mayer, David M. Nichols, Elli Papadopoulou, Thomas Pasquier, Tony Ross-Hellauer, Michael Schulte-Mecklenbeck, Dan Sholler, Tobias Steiner, Pawel Szczesny, Andy Turner

Copyright, Fair Use, Scholarly Communication, etc.

This document aims to agree on a broad, international strategy for the implementation of open scholarship that meets the needs of different national and regional communities but works globally.

Scholarly research can be idealised as an inspirational process for advancing our collective knowledge to the benefit of all humankind. However, current research practices often struggle with a range of tensions, in part due to the fact that this collective (or “commons”) ideal conflicts with the competitive system in which most scholars work, and in part because much of the infrastructure of the scholarly world is becoming largely digital. What is …

Sampling Related Individuals Within Ponds Biases Estimates Of Population Structure In A Pond‐Breeding Amphibian, Kyle A. O'Connell, Kevin P. Mulder, Jose Maldonado, Kathleen L. Currie, Dennis M. Ferraro

School of Natural Resources: Faculty Publications

Effective conservation and management of pond‐breeding amphibians depends on the accurate estimation of population structure, demographic parameters, and the influence of landscape features on breeding‐site connectivity. Population‐level studies of pond‐breeding amphibians typically sample larval life stages because they are easily captured and can be sampled nondestructively. These studies often identify high levels of relatedness between individuals from the same pond, which can be exacerbated by sampling the larval stage. Yet, the effect of these related individuals on population genetic studies using genomic data is not yet fully understood. Here, we assess the effect of within‐pond relatedness on population and landscape …

Checklist Of Bees (Hymenoptera: Apoidea) From Small Diversified Vegetable Farms In South-Western Montana, Casey M. Delphia, Terry Griswold, Elizabeth G. Reese, Kevin M. O'Neill, Laura A. Burkle

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background

Over three years (2013-2015), we sampled bees using nets and bowl traps on four diversified vegetable farms in Gallatin County, Montana, USA, as part of a study evaluating the use of wildflower strips for supporting wild bees and crop pollination services on farmlands (Delphia et al. In prep). We document 202 species and morphospecies from 32 genera within five families, of which 25 species represent the first published state records for Montana. This study increases our overall understanding of the distribution of wild bee species associated with agroecosystems of the northern US Rockies, which is important for efforts aimed …

Effect Of Cooled Perches On Performance, Plumage Condition, And Foot Health Of Caged White Leghorn Hens Exposed To Cyclic Heat, J Y. Hu, P Y. Hester, M M. Makagon, Y Xiong, R S. Gates, H W. Cheng

Department of Animal Science: Faculty Publications

We examined the effects of water-chilled perches as cooling devices on hen performance during 2 summers using daily cyclic heat. White Leghorns, 17 wk of age, were assigned to 36 cages arranged into 6 banks. The banks were assigned to cooled perches, air perches, and no perches resulting in 2 replicate banks and 12 cages per treatment. Chilled water (10^◦C) was circulated through the cooled perches during heat episodes. Daily cyclic heat of 35^◦C was applied from 0600 to1800 h with a lowering of temperature to 28^◦C from 1800 to 0600 h during the …

Assessing Insect Flight Behavior In The Laboratory: A Primer On Flight Mill Methodology And What Can Be Learned, Steven E. Naranjo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Dispersal is a key component in the population ecology and dynamics of insects and remains one of the most difficult and intractable ecological processes to study in the field. As a consequence, many researchers have looked to laboratory methods for investigating the myriad factors that govern and impact an insect’s ability to move within its environment. A key tool in this effort since at least the early 1950s has been the insect flight mill. Nearly 260 studies have been published using flight mills covering 214 species in 61 families and 9 orders. This review explores the methodology and technology of …

Genome-Wide Characterization, Molecular Evolution And Mexpression Profiling Of The Metacaspases In Potato (Solanum Tuberosum L.), Nehal Dubey, Maitri Trivedi, Suresh Varsani, Vishal Vyas, Manisha Farsodia, Sunil Kumar Singh

Department of Entomology: Faculty Publications

Metacaspases are distant relatives of animal caspases found in plants, protozoa and fungi. Some recent studies have demonstrated that metacaspases are involved in regulating the developmental and environmentally induced programmed cell death in plants. In this study, we identified metacaspase gene family in potato (Solanum tuberosum L.) and analyzed their expression pattern in various developmental tissues and stress responses of plants. There were eight metacaspase genes identified in the Peptidase (Cysteine protease) C14 family and based upon sequence alignment and phylogenetic analysis, a systematic nomenclature of potato metacaspases (SotubMCs) has been proposed. Three of the eight candidate genes showing homology …

Photosynthesis, Yield And Raw Material Quality Of Sugarcane Injured By Multiple Pests, José A.S. Rossato Jr, Leonardo L. Madaleno, Márcia J.R. Mutton, Leon Higley, Odair A. Fernandes

School of Natural Resources: Faculty Publications

Understanding sugarcane (Saccharum spp.) response to multiple pest injury, sugarcane borer (Diatraea saccharalis) and spittlebug (Mahanarva fimbriolata), is essential to make better management decisions. Moreover, the consequences of both pests on the sugarcane raw material quality have not yet been studied. A field experiment was performed in São Paulo State, Brazil, where sugarcane plants were exposed to pests individually or in combination. Plots consisted of a 2-m long row of caged sugarcane plants. Photosynthesis was measured once every 3 months (seasonal measurement). Yield and sugar production were assessed. The measured photosynthesis rate was negatively affected by both borer and spittlebug …

Residues Of Thiamethoxam And Mefenoxam In Vegetative And Floral Tissue Of Soybean At The Early Reproductive Stage Resulting From Seed Treatments, Carolina Camargo, Daniel D. Snow, Sathaporn Onanong, Thomas Hunt, Blair Siegfried

Department of Entomology: Faculty Publications

Thiamethoxam with mefenoxam is the most widely used neonicotinoid insecticide/fungicide mixture applied to soybean (Glycine max [L]) as seed treatments. Based on the systemic nature of thiamethoxam and mefenoxam, residues of this insecticide/fungicide mixture may be present in soybean vegetative and floral tissue and negatively impact beneficial insects. Although neonicotinoids are often applied in combination with systemic fungicides, the research on ecological risks of neonicotinoids has been focused on the analysis of these compounds without considering their interaction with other agrochemicals. The objective of this study was to identify the concentration of thiamethoxam and mefenoxam in soybean flowers and …

Metacognitive Deficits And Social Functioning In Schizophrenia Across Symptom Profiles: A Latent Class Analysis, Emily C. Gagen, Aieyat B. Zalzala, Jesse Hochheiser, Ashley Schnakenberg Martin, Paul H. Lysaker

Department of Psychology: Faculty Publications

Functional deficits are a hallmark of schizophrenia spectrum disorders, but much debate still exists over why and how they originate. One model suggests that disturbances in social functioning are a result of metacognitive deficits or a failure to integrate information to form more complex ideas of themselves and others. It is unclear if this social dysfunction is present across different symptom presentations. We examined the relationship of metacognition, symptoms, and social functioning among a sample of adults with schizophrenia spectrum disorders (N ¼ 334). A latent class analysis produced a four-class model. Groups were classified as follows: diffuse symptoms/moderately impaired …