Digital Commons Network^™

Microbial Community Of Saline, Alkaline Lakes In The Nebraska Sandhills Based On 16s Rrna Gene Amplicon Sequence Data, Nicole A. Fiore, David D. Dunigan, Julie J. Shaffer, Ryan Roberts, Sanjay Antony-Babu, Bradley A. Plantz, Kenneth W. Nickerson, Andrew K. Benson, Karrie A. Weber

School of Biological Sciences: Faculty Publications

The Nebraska Sandhills region contains over 1,500 geochemically diverse interdunal lakes, some of which are potassium rich, alkaline, and hypersaline. Here, we report 16S rRNA amplicon pyrosequencing data on the water and sediment microbial communities of eight alkaline lakes in the Sandhills of western Nebraska.

Differential Gene Expression In Response To Ecry3.1ab Ingestion In An Unselected And Ecry3.1abselected Western Corn Rootworm (Diabrotica Virgifera Virgifera Leconte) Population, Zixiao Zhao, Lisa N. Meihls, Bruce E. Hibbard, Tieming Ji, Christine G. Elsik, Kent S. Shelby

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Diabrotica virgifera virgifera LeConte, the western corn rootworm (WCR) is one of the most destructive pests in the U.S. Corn Belt. Transgenic maize lines expressing various Cry toxins from Bacillus thuringiensis have been adopted as a management strategy. However, resistance to many Bt toxins has occurred. To investigate the mechanisms of Bt resistance we carried out RNA-seq using Illumina sequencing technology on resistant, eCry3.1Ab-selected and susceptible, unselected, whole WCR neonates which fed on seedling maize with and without eCry3.1Ab for 12 and 24 hours. In a parallel experiment RNA-seq experiments were conducted when only the midgut of neonate WCR was …

Environmental Transmission Of Toxoplasma Gondii: Oocysts In Water, Soil And Food, Karen Shapiro, Lillian Bahia-Oliveira, Brent Dixon, Aurélien Dumètre, Luz A. De Wit, Elizabeth Vanwormer, Isabelle Villena

School of Veterinary and Biomedical Sciences: Faculty Publications

Toxoplasma gondii is a zoonotic protozoan parasite that can cause morbidity and mortality in humans, domestic animals, and terrestrial and aquatic wildlife. The environmentally robust oocyst stage of T. gondii is fundamentally critical to the parasite's success, both in terms of its worldwide distribution as well as the extensive range of infected intermediate hosts. Despite the limited definitive host species (domestic and wild felids), infections have been reported on every continent, and in terrestrial as well as aquatic environments. The remarkable resistance of the oocyst wall enables dissemination of T. gondii through watersheds and ecosystems, and long-term persistence in diverse …

Convergence Science In The Anthropocene: Navigating The Known And Unknown, David G. Angeler, Craig R. Allen, Ana Carnaval

School of Natural Resources: Faculty Publications

1. Rapidly changing ecological and social systems currently pose significant societal challenges. Navigating the complexity of social-ecological change requires ap- proaches able to cope with, and potentially solve, both foreseen and unforeseen societal challenges.

2. The emergent field of convergence addresses the intricacies of such challenges, and is thus relevant to a broad range of interdisciplinary issues.

3. This paper suggests a way to conceptualize convergence research. It discusses how it relates to two major societal challenges (adaptation, transformation), and to the generation of policy-relevant science. It also points out limitations to the further development of convergence research.

Development Of A Nitrogen Recommendation Tool For Corn Considering Static And Dynamic Variables, Laila A. Puntel, Agustin Pagani, Sotirios V. Archontoulis

Department of Agronomy and Horticulture: Faculty Publications

Many soil and weather variables can affect the economical optimum nitrogen (N) rate (EONR) for maize. We classified 54 potential factors as dynamic (change rapidly over time, e.g. soil water) and static (change slowly over time, e.g. soil organic matter) and explored their relative importance on EONR and yield prediction by analyzing a dataset with 51 N trials from Central-West region of Argentina. Across trials, the average EONR was 113 ± 83 kg N ha⁻¹ and the average optimum yield was 12.3 ± 2.2 Mg ha⁻¹, which is roughly 50% higher than the current N rates used …

Emigration Of Hatchery‐Reared Pallid Sturgeon, Scaphirhynchus Albus (Forbes And Richardson), Through A Missouri River Dam, Landon L. Pierce, Dane A. Shuman, Daniel A. James, Kirk D. Steffensen, Ryan Wilson, Kyle Winders

Nebraska Game and Parks Commission: Staff Research Publications

The middle Missouri River (MMR; Fort Randall Dam, SD to Gavins Point Dam, NE‐SD) is stocked with hatchery‐reared pallid sturgeon, Scaphirhynchus albus (Forbes and Richardson), from upper Missouri River broodstock to aid recovery of this federally endangered species. Emigration of these fish through Gavins Point Dam restores genetic connectivity that likely existed pre‐impoundment but could lead to outbreeding depression in the future. Recapture data of hatchery‐reared pallid sturgeon stocked in the MMR were evaluated to improve understanding of pallid sturgeon emigration. From 2004 to 2015, 219 emigrants were caught: 4 stocked at age ≥2 years and 215 stocked at age …

Tlr10 Senses Hiv-1 Proteins And Significantly Enhances Hiv-1 Infection, Bethany M. Henrick, Xiao-Dan Yao, Muhammad Atif Zahoor, Alash’Le Abimiku, Sophia Osawe, Kenneth L. Rosenthal

Department of Food Science and Technology: Faculty Publications

Toll-like receptors (TLRs) play a crucial role in innate immunity and provide a first line of host defense against invading pathogens. Of the identified human TLRs, TLR10 remains an orphan receptor whose ligands and functions are poorly understood. In the present study, we sought to evaluate the level of TLR10 expression in breastmilk (BM) and explore its potential function in the context of HIV-1 infection. We evaluated HIV-1-infected (Nigerian: n = 40) and uninfected (Nigerian: n = 27; Canadian: n = 15) BM samples for TLR expression (i.e., TLR10, TLR2, and TLR1) and report here that HIV-1-infected BM from Nigerian …

Soft , Multilayered Electronics For Wearable Devices And Methods To Produce The Same, Carmel Majidi, Michael D. Bartlett, Eric J. Markvicka

Department of Mechanical and Materials Engineering: Faculty Publications

Disclosed herein is an efficient fabrication approach to create highly customizable wearable electronics through rapid laser machining and adhesion - controlled soft materials assembly . Well - aligned , multi - layered materials can be created from 2D and 3D elements that stretch and bend while seamlessly integrating with rigid components such as micro chip integrated circuits ( IC ) , discrete electrical components , and interconnects . These techniques are applied using commercially available materials . These materials and methods enable custom wearable electronics while offering versatility in design and functionality for a variety of bio - monitor ing …

Redescription Of Anaschisma (Temnospondyli: Metoposauridae) From The Late Triassic Of Wyoming And The Phylogeny Of The Metoposauridae, Bryan M. Gee, William G. Parker, Adam D. Marsh

United States National Park Service: Publications

Metoposaurids are non-marine temnospondyls that are among the most common constituents of Late Triassic deposits, but despite their abundance, the evolutionary relationships of the group are poorly resolved and have not been fully addressed with modern phylogenetic methods. The genus Anaschisma is one of a number of poorly resolved metoposaurid taxa and was erected to describe two species from the Popo Agie Formation (Carnian) in Wyoming: Anaschisma browni and Anaschisma brachygnatha. Since being named, the genus has been repeatedly synonymized and separated with other taxa in the context of broader revisions of the Metoposauridae. At present, Anaschisma is considered …

Vapor Delivery Of Plant Essential Oils Alters Pyrethroid Efficacy And Detoxification Enzyme Activity In Mosquitoes, Scott T. O'Neal, Ellis J. Johnson, Leslie Catherine Rault, Troy D. Anderson

Department of Entomology: Faculty Publications

The use of synthetic insecticides to limit the spread of mosquito-borne disease faces a number of significant challenges, including insecticide resistance, concerns related to the environmental impact of widespread insecticide use, as well as slowed development of new insecticide chemistries. One important alternative to broadcast insecticides is the use of personal protection strategies to limit contact with vector species, including the use of spatial repellents that can employ synthetic pyrethroids or botanical products to effect control. A currently underexplored area of research involves the investigation of botanical products for their potential to serve as insecticide synergists when delivered as a …

The Accelerating Influence Of Humans On Mammalian Macroecological Patterns Over The Late Quaternary, Felisa A. Smith, Rosemary E. Elliott Smith, S. Kathleen Lyons, Jonathan L. Payne, Amelia Villaseñor

School of Biological Sciences: Faculty Publications

The transition of hominins to a largely meat-based diet ~1.8 million years ago led to the exploitation of other mammals for food and resources. As hominins, particularly archaic and modern humans, became increasingly abundant and dispersed across the globe, a temporally and spatially transgressive extinction of large-bodied mammals followed; the degree of selectivity was unprecedented in the Cenozoic fossil record. Today, most remaining large-bodied mammal species are confined to Africa, where they coevolved with hominins. Here, using a comprehensive global dataset of mammal distribution, life history and ecology, we examine the consequences of “body size downgrading” of mammals over the …

Mitotic Recombination And Rapid Genome Evolution In The Invasive Forest Pathogen Phytophthora Ramorum, Angela L. Dale, Nicolas Feau, Sydney E. Everhart, Braham Dhillon, Barbara Wong, Julie Sheppard, Guillaume J. Bilodeau, Avneet Brar, Javier F. Tabima, Danyu Shen, Clive M. Brasier, Brett M. Tyler, Niklaus J. Grünwald, Richard C. Hamelin

Department of Plant Pathology: Faculty Publications

Invasive alien species often have reduced genetic diversity and must adapt to new environments. Given the success of many invasions, this is sometimes called the genetic paradox of invasion. Phytophthora ramorum is invasive, limited to asexual reproduction within four lineages, and presumed clonal. It is responsible for sudden oak death in the United States, sudden larch death in Europe, and ramorum blight in North America and Europe. We sequenced the genomes of 107 isolates to determine how this pathogen can overcome the invasion paradox. Mitotic recombination (MR) associated with transposons and low gene density has generated runs of homozygosity (ROH) …

Improving Risk Assessment Of The Emergence Of Novel Influenza A Viruses By Incorporating Environmental Surveillance, Kim M. Pepin, Matthew W. Hopken, Susan A. Shriner, Erica Spackman, Zaid Abdo, Colin Parrish, Steven Steven, James O. Lloyd-Smith, Antoinette J. Piaggio

United States Department of Agriculture Wildlife Services: Staff Publications

Reassortment is an evolutionary mechanism by which influenza A viruses (IAV) generate genetic novelty. Reassortment is an important driver of host jumps and is widespread according to retrospective surveillance studies.However, predicting the epidemiological risk of reassortant emergence in novel hosts from surveillance data remains challenging. IAV strains persist and cooccur in the environment, promoting co-infection during environmental transmission. These conditions offer opportunity to understand reassortant emergence in reservoir and spillover hosts. Specifically, environmental RNA could provide rich information for understanding the evolutionary ecology of segmented viruses, and transform our ability to quantify epidemiological risk to spillover hosts. However, significant challenges …

H/L Ratio As A Measurement Of Immune Function In Terrapene Ornata Species, Vesa Govori

Honors Program: Senior Projects (Public)

White blood cells are a key part of the immune system, and changes in white cell count can reveal the overall state of health and the immune systems functional ability of a species. White blood such as heterophils and lymphocytes are essential for being able to fight off infections. Analyzing different cell ratios has been commonly done before as a way to observe immune function in bird species as well as other species. Understanding that there are different functions between the different white blood cells provides an extra interesting perspective to what could be happening in the immune system function …

Hydrodistillation Extraction Kinetics Regression Models For Essential Oil Yield And Composition In Juniperus Virginiana, J. Excelsa, And J. Sabina, Ivanka B. Semerdjieva, Santosh Shiwakoti, Charles L. Cantrell, Valtcho D. Zheljazkov, Tess Astatkie, Vicki L. Schlegel, Tzenka Radoukova

Department of Food Science and Technology: Faculty Publications

The chemical profile and antioxidant capacity of Juniperus virginiana, J. excelsa, and J. sabina essential oil (EO) fractions as a function of time was the subject of this study. The hypothesis was that, capturing EO in sequential timeframes during hydrodistillation would generate fractions containing unique compositions and antioxidant capacity. In J. virginiana, the highest limonene (43%) was found in the 0–5 min oil fraction, with safrole (37%) being highest in the 10–20 and 20–40 min fractions, and elemol (34%) being highest in the 160–240 min fraction. In J. excelsa, α-pinene (34-36%) was the highest in the 0–5 min …

Reflecting On Efforts To Design An Inclusive Citizen Science Project In West Baltimore, Amanda E. Sorensen, Rebecca C. Jordan, Shannon L. Ladeau, Dawn Biehler, Sacoby Wilson, John-Henry Pitas, Paul T. Leisnham

School of Natural Resources: Faculty Publications

Citizen science (CS) has been an increasingly utilized means by which scientists leverage members of the public to increase the amount of data collected and analyzed. However, the underrepresentation of individuals from certain socio-cultural groups can have consequences that can manifest in the scientific outcomes of those CS projects such as biases in the data. Additionally, this underrepresentation can potentially affect long-term viability and support of CS as a community of practice. CS programs that promote greater inclusivity would likely provide opportunities for communities to define, investigate, and address pressing issues in collaboration with professional scientists. In this paper we …

Using Artificial Neural Networks To Predict Future Dryland Responses To Human And Climate Disturbances, C.E. Buckland, R.M. Bailey, D.S.G. Thomas

Department of Earth and Atmospheric Sciences: Faculty Publications

Land degradation and sediment remobilisation in dryland environments is considered to be a significant global environmental problem. Given the potential for currently stabilised dune systems to reactivate under climate change and increased anthropogenic pressures, identifying the role of external disturbances in driving geomorphic response is vitally important. We developed a novel approach, using artificial neural networks (ANNs) applied to time series of historical reactivation-deposition events from the Nebraska Sandhills, to determine the relationship between historic periods of sand deposition in semi-arid grasslands and external climatic conditions, land use pressures and wildfire occurrence. We show that both vegetation growth and sediment …

A Human Papillomavirus-Independent Cervical Cancer Animal Model Reveals Unconventional Mechanisms Of Cervical Carcinogenesis, Chunbo He, Xiangmin Lv, Cong Huang, Peter C. Angeletti, Guohua Hua, Jixin Dong, Jin Zhou, Zhengfeng Wang, Bowen Ma, Xingcheng Chen, Paul F. Lambert, Bo R. Rueda, John S. Davis, Cheng Wang

Nebraska Center for Virology: Faculty Publications

HPV infections are common in healthy women and only rarely cause cervical cancer, suggesting that individual genetic susceptibility may play a critical role in the establishment of persistent HPV infection and the development of cervical cancer. Here, we provide convincing in vitro and in vivo evidence showing that differential expression and activation of YAP1 oncogene determine individual susceptibility to HPV infection and cervical carcinogenesis. We found that hyperactivation of YAP1 in mouse cervical epithelium was sufficient to induce invasive cervical cancer. Cervical epithelial cell-specific HPV16 E6/E7 and YAP1 double-knockin mouse model demonstrated that high-risk HPV synergized with hyperactivated YAP1 to …

Emerging Themes From The Esa Symposium Entitled “Pollinator Nutrition: Lessons From Bees At Individual To Landscape Levels”, Vanessa Corby-Harris, Julia H. Bowsher, Morgan Carr-Markell, Mark J. Carroll, Mary Centrella, Steven C. Cook, Margaret Couvillon, Gloria Degrandi-Hoffman, Adam Dolezal, Julia C. Jones, Christina L. Mogren, Clint R. V. Otto, Pierre Lau, Juliana Rangel, Roger Schürch, Ashley St. Clair

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Pollinator populations are declining (Biesmeijer et al., 2006; Brodschneider et al., 2018; Cameron et al., 2011; Goulson, Lye, & Darvill, 2008; Kulhanek et al., 2017; National Research Council, 2007; Oldroyd, 2007), and both anecdotal and experimental evidence suggest that limited access to high quality forage might play a role (Carvell, Meek, Pywell, Goulson, & Nowakowski, 2007; Deepa et al., 2017; Goulson, Nicholls, Botias, & Rotheray, 2015; Potts et al., 2003, 2010; Vanbergen & The Insect Pollinators Initiative, 2013; Vaudo, Tooker, Grozinger, & Patch, 2015; Woodard, 2017). Multiple researchers are earnestly addressing this topic in a diverse array of insect-pollinator systems. …

Proceedings Of The 46th Annual Meeting Of The Southern Soybean Disease Workers (March 6-7, 2019, Pensacola Beach, Florida), Carl Bradley, Heather M. Kelly, Burt Bluhm, Danise Beadle, Patricia Bollich, Trey Price, Tom Allen

Southern Soybean Disease Workers: Conference Proceedings

Contents

List of SSDW Officers

Agenda for March 6-7, 2019

Abstracts for presented/contributed papers

Evaluating phytotoxicity in response to fungicide by adjuvant combinations. TW Allen and TH Wilkerson

Effect of delayed harvest on the seed quality of commercial soybean cultivars. JC Rupe, JA Rojas, R Holland, SR Segalin, RD Bond, and JA Still

Virome of Macrophomina phaseolina isolates collected from soybean fields in Mississippi. Nina Aboughanem-Sabanadzovic, Tessie Wilkerson, Tom Allen, and Sead Sabanadzovic

Genomic evidence for interspecific hybridization in Cercospora cf. flagellaris strains associated with Cercospora leaf blight of soybean. Alex Zaccaron, Kona Swift, Ahmad Fakhoury, and Burt Bluhm

Identification …

Is One Secure Attachment Enough? Infant Cortisol Reactivity And The Security Of Infant-Mother And Infant-Father Attachments At The End Of The First Year, Patty X. Kuo, Ekjyot K. Saini, Elizabeth Tengelitsch, Brenda L. Volling

Department of Child, Youth, and Family Studies: Faculty Publications

Attachment security is theorized to shape stress reactivity, but extant work has failed to find consistent links between attachment security to mothers and infant cortisol reactivity. We examined family configurations of infant-mother and infant-father attachment security in relation to infant cortisol reactivity. One-year old infants (N = 180) participated in the Strange Situation with mothers and fathers in two counterbalanced lab visits, one month apart (12 and 13 months). Infants with secure attachments only to their fathers and not their mothers had higher cortisol levels than infants with a secure attachment to mother and also exhibited a blunted cortisol response …

The Nebraska Bird Review, Volume 87 March 2019 Number 1

Nebraska Bird Review

NOU Annual Treasurer's Report for 2018, by Jan Johnson … 2

Winter Field Report, Dec. 2018 - Feb. 2019, by W. Ross Silcock …3

2018 - 2019 Christmas Bird Counts, by Don Paseka …15-16 & 25-38

Annual Color Photo Section …17-24

Subscription and Organization Information … 39

New State Record For Coniopteryx Tineiformis Curtis (Neuroptera: Coniopterygidae), Lawrence Barringer

Insecta Mundi

Coniopteryx tineiformis Curtis (Neuroptera: Coniopterygidae) is reported from Pennsylvania for the first time, a new state record. The Pennsylvania Department of Agriculture’s collection holdings were reviewed and four specimens from two counties were found. These records fill in a gap in this species’ reported range, despite no records of it from adjacent states.

2018-2019 Christmas Bird Counts, Don Paseka

Nebraska Bird Review

Eighteen counts were held during the 2018-19 Christmas Bird Count season. This is two more than were done last season, which was the first time that as many as sixteen counts were held. This year no counts were postponed or cancelled, and the Beaver Valley Count was revived after a four year hiatus. Twelve of the counts were done during the first week of the count period. While the conditions for these early counts were mild and dry, heavy precipitation in late fall coupled with an early onset of cold weather in the first part of December resulted in little …

Annual Color Photo Section (2019)

Nebraska Bird Review

LeConte's Sparrow, Little Salt Fork Marsh, Lancaster Co., 22 Oct. 2018. Photo by Ruth Stearns.

Harris's Sparrow and Whitecrowned Sparrow, Polk Co., 29 Oct. 2018. Photo by Jan Johnson.

Vesper Sparrow, Chimney Rock Road, Morrill Co., 18 Sept. 2017. Photo by Clem Klaphake.

Fox Sparrow, Mahoney State Park, Cass Co., 25 Feb 2019. Photo by Elizabeth Winter.

Adult Bald Eagle, Chalco Hills Recreation Area, Sarpy Co., 25 Jan. 2019. Photo by Mike Schilmoeller.

White-winged Dove, photographer's yard,Wahoo, Saunders Co., 19 May 2019. Photo by Joe Freeborn.

Juvenile Cooper's Hawk, midtown Omaha, Douglas Co,. 18 Aug. 2014. Photo by Craig Crews. …

Characterization Of Pyrethroid Resistance In The Western Corn Rootworm Diabrotica Virgifera Virgifera Leconte, Dariane Sagaseta De Oliveira Souza

Department of Entomology: Dissertations, Theses, and Student Research

The western corn rootworm (WCR), Diabrotica virgifera virgifera Le Conte (Coleoptera: Chrysomelidae), is a major pest of maize (Zea mays L.) in the United States and has continuously challenged our ability to develop sustainable pest management solutions. Resistance to chemical insecticides, crop rotation, and most recently transgenic plants that express toxins derived from Bacillus thuringiensis Berliner (Bt) have been well documented among WCR populations. Furthermore, US regulatory insecticide prohibitions and the lack of new active ingredients have reduced the options for WCR control. Pyrethroid insecticides are one of the few remaining chemical options. Therefore, the recent confirmation …

Escaping Death: Naloxone's Chemical Nature And Potential To Combat The Opioid Epidemic, Abigale Miller

Honors Program: Senior Projects (Public)

Naloxone is a life-saving drug with the ability to reverse an opioid overdose. As the opioid epidemic’s death toll rises, we can turn to Naloxone as a tool to combat the crisis. The epidemic, born of corruption, has a wide reach among the people of the United States, with especially firm grasps on middle-aged people, sufferers of chronic pain, white Americans and those living in the eastern portion of the country. Naloxone’s elegant design saves lives by effectively competing for a position on an opioid biding receptor in the brain to almost instantly end an overdose and restore normal breathing. …

Does My Vote Count? Analyzing The Motivations Of American Voters And The Obstacles They Face, Kalika Mahato

Honors Program: Senior Projects (Public)

One of the most indispensable rights Americans are promised is the opportunity to vote at the polls. After the women’s suffrage and civil rights movements, all American citizens above 18 and who haven’t committed a felony have the right to vote. The election process in America is viewed by many as egalitarian. However, upon a rudimentary examination into the election process, it becomes clear that this equality that America promotes is consistently at battle with classism and hierarchy. Every election, thousands of eligible voters do not vote because of inadequate information and support, barriers in the process, and other forms …

Student Perceptions Of Reasons For Lecture And Active Learning, Calan Koch

Honors Program: Senior Projects (Public)

With perceived student resistance to active learning in the classroom, instructors are hesitant to implement such methods into their classroom structure despite how effective they may be. This research seeks to understand student perceptions related to the transition to higher prevalence of active learning techniques. We aimed to find answers to how students perceive the ideal classroom to be structured, what reasons they perceive for lecture and active learning components, and possible explanations to concerns of groupwork during class time. We analyzed 64 interviews with undergraduate biology students at the University of Nebraska- Lincoln, developed coding rubrics from commonly found …

Dietary Polyphenols And Their Perceived Health Benefits, Stephanie Russell

Honors Program: Senior Projects (Public)

This paper will consist of a compilation of information on polyphenol classes, dietary sources, and potential health benefits. Polyphenols can be classified as flavonoids, phenolic acids, lignans, or stilbenes. These molecules consist of over 500 plant metabolites that come from numerous food sources including various fruits, vegetables, grains, and beverages such as coffee, tea, and wine. The quantity of polyphenols in food vary greatly, as does their bioavailability for use in the body after consumption. Due to antioxidant and anti-inflammatory properties, polyphenols are being studied for effects on reducing the risk of cardiovascular disease. This paper will focus on structural …