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Persistence And Mitigation Of Antibiotic Resistance In Manure And Manure-Amended Soils, Mara J. Zelt

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

The emergence of antibiotic resistance (AR) is a growing global threat to human and animal health. The work described here asses the AR mitigation potential of management strategies at critical control points in livestock production, and agricultural land management as well as the effectiveness of a communication strategy to convey research-based information to empower behavioral change that could mitigate AR.

The first study evaluates the impact of beef cattle diet management strategies on AMR prevalence in manure. Two treatments – forage concentration and essential oils – in cattle diets were evaluated for their impact on AMR bacteria in feedlot manure. …

Flex-Ro: A Robotic High Throughput Field Phenotyping System, Joshua N. Murman

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Research in agriculture is critical to developing techniques to meet the world’s demand for food, fuel, fiber, and feed. Optimization of crop production per unit of land requires scientists across disciplines to collaborate and investigate new areas of science and tools for data collection. The use of robotics has been adopted in several industries to supplement labor, and accurately perform repetitious tasks. However, the use of autonomous robots in commercial agricultural production is still limited. The Flex-Ro (Flexible structured Robotic platform) was developed for use in large area fields as a multipurpose tool to perform monotonous agricultural tasks.

This work …

Xenobiotic Exposure Requires Mitochondrial Metabolism For Redox Homeostasis And Survival In Astrocytes, Jordan Rose

Department of Biochemistry: Dissertations, Theses, and Student Research

Astrocytes are integral components of glutamatergic neurotransmission, providing essential metabolic processes for neuronal homeostasis and acting as the first line of defense against xenobiotics crossing the blood brain barrier. Arsenic is a xenobiotic with widespread natural and industrial prevalence, and has been linked to impaired neurodevelopment and neuronal death. Given the integrated metabolic nature of astrocytes and neurons, we sought to explore how arsenic impacts astrocyte metabolism in order to better understand the mechanisms of xenobiotic toxicity in the mammalian brain.

We demonstrate that astrocyte viability depends upon de novoglutathione (GSH) synthesis during arsenic exposure, and sub-lethal arsenic exposure …

Motherhood And The Periodical Press: The Myth And The Medium, Susan A. Malcom

Department of English: Dissertations, Theses, and Student Research

In this study, I utilize close readings of the periodically published works of three women writers – Kate Chopin, Frances Ellen Watkins Harper, and Elia Peattie –through the lenses of historical/biographical, affective, and biosocial theories. Examining these works against the backdrop of America’s mythologized mother exposes the social ubiquity of the myth and the realities of motherhood nineteenth-century women experienced.

Chapter one examines the mythological nature of American motherhood as it evolved from a politically and socially nuanced Republican Mother and the role of American periodicals as a medium of perpetuating that myth. Historically, American motherhood was an extended function …

Changes In The Diet And Body Size Of A Small Herbivorous Mammal (Hispid Cotton Rat, Sigmodon Hispidus) Following The Late Pleistocene Megafauna Extinction, Catalina P. Tomé, Emma A. Elliott Smith, S. Kathleen Lyons, Seth D. Newsome, Felisa A. Smith

School of Biological Sciences: Faculty Publications

The catastrophic loss of large-bodied mammals during the terminal Pleistocene likely led to cascading effects within communities. While the extinction of the top consumers probably expanded the resources available to survivors of all body sizes, little work has focused on the responses of the smallest mammals. Here, we use a detailed fossil record from the southwestern United States to examine the response of the hispid cotton rat Sigmodon hispidus to biodiversity loss and climatic change over the late Quaternary. In particular, we focus on changes in diet and body size. We characterize diet through carbon (δ¹³C) and nitrogen …

Preface For Special Section On Archaeoparasitology: A Global Perspective On Ancient Parasites And Current Research Projects, Jong Yil Chai, Min Sea, Karl Reinhard, Dong Hoon Shin

Karl Reinhard Publications

Archaeoparasitological research has rapidly developed in recent years, entering a new stage of improved understanding of our ancestors’ parasitic infections across the world. This progress is based on a variety of recently developed research techniques. During a period of rapid innovation since 2016, there has been no much opportunity for parasitologists to gather and share in-depth views about this novel research field. This was remedied by the 14th International Congress of Parasitology (ICOPA) held in Korea (Aug. 19-24, 2018; EXCO, Daegu). For archaeoparasitologists, ICOPA provided a unique opportunity to gather and exchange research and ideas. The participation was unprecedented in …

Confusing A Pollen Grain With A Parasite Egg: An Appraisal Of “Paleoparasitological Evidence Of Pinworm (Enterobius Vermicularis) Infection In A Female Adolescent Residing In Ancient Tehran”, Morgana Camacho, Karl Reinhard

Karl Reinhard Publications

There is often the risk of confusing pollen grains with helminth eggs from archaeological sites. Thousands to millions of pollen grains can be recovered from archaeological burial sediments that represent past ritual, medication and environment. Some pollen grain types can be similar to parasite eggs. Such a confusion is represented by the diagnosis of enterobiasis in ancient Iran. The authors of this study confused a joint-pine (Ephedra spp.) pollen grain with a pinworm egg. This paper describes the specific Ephedra pollen morphology that can be confused with pinworm eggs.

Pinworm Infection At Salmon Ruins And Aztec Ruins: Relation To Pueblo Iii Regional Violence, Karl Reinhard, Morgana Camacho

Karl Reinhard Publications

The study of coprolites has been a theme of archaeology in the American Southwest. A feature of archaeoparasitology on the Colorado Plateau is the ubiquity of pinworm infection. As a crowd parasite, this ubiquity signals varying concentrations of populations. Our recent analysis of coprolite deposits from 2 sites revealed the highest prevalence of infection ever recorded for the region. For Salmon Ruins, the deposits date from AD 1140 to 1280. For Aztec Ruins, the samples can be dated by artifact association between AD 1182-1253. Both sites can be placed in the Ancestral Pueblo III occupation (AD 1100-1300), which included a …

Identification Of The Kaposi's Sarcoma-Associated Herpesvirus (Kshv) Surface Glycoprotein Targets Of Human Kshv-Specific Neutralizing Antibody Responses, Yasaman Mortazavi

School of Biological Sciences: Dissertations, Theses, and Student Research

Kaposi’s sarcoma-associated herpesvirus (KSHV), also known as human herpesvirus 8 (HHV-8), is the etiological agent of Kaposi’s sarcoma (KS), and is also associated with two B cell malignancies, primary effusion lymphoma and multicentric Castleman's disease. The distribution of KSHV varies globally with high prevalence in some areas of sub-Saharan Africa (SSA), where seroprevalence can be as high as 80%. It is estimated that nearly 44,000 new cases of KS emerge annually globally, with the highest incidents occurring in Africa, where KSHV is endemic. Currently, there is no prophylactic vaccine against KSHV, and efforts to develop prophylactic vaccines have been limited. …

The Prairie Naturalist, Vol. 51, Issue 2, December 2019

The Prairie Naturalist

45 EDITOR’S NOTE: Final Thoughts as Editor-in-Chief, by Christopher N. Jacques

RESEARCH ARTICLES

47 Bats of the Loess Hills Ecoregion of Southeast Nebraska, by Virgil Brack, Jr., Dale W. Sparks, and Darwin C. Brack

58 Serological Survey and Pathogen Exposure of Adult Female White-tailed Deer in the Western Dakotas, by Katherine L. Moratz, Bailey S. Gullikson, Eric S. Michel, Daniel M. Grove, Jonathan A. Jenks, and William F. Jensen

68 Factors Limting Reintroduced Plains Topminnow, Fundulus sciadicus, Populations in Central Great Plains Streams, by Joseph D. Thiessen, Keith D. Koupal, and Casey W. Schoenebeck

NOTES

77 Recent Observations of Water …

Factors Limiting Reintroduced Plains Topminnow, Fundulus Sciadicus, Populations In Central Great Plains Streams, Joseph Thiessen, Keith D. Koupal, Casey W. Schoenebeck

The Prairie Naturalist

The plains topminnow (Fundulus sciadicus) is an endemic Great Plains stream fish that has experienced declines in geographic range and local abundance. Due to these declines, the species has been considered for federal protection and designated with conservation status in states throughout its historic range. The reasons for declines are likely similar to hypothesized factors for other endemic stream fish declines in the Great Plains. To investigate potential limiting factors a suite of 17 historic sites with reintroduced plains topminnow populations across Nebraska were evaluated for current populations and if plains topminnow were absent, additional fish were introduced. …

Review Of Grasslands And Climate Change By David J. Gibson And Jonathan A. Newman, Neal D. Noemuth

The Prairie Naturalist

The last decade has seen an explosion of information about climate change, some of which is contradictory, much of which is confusing, and the entirety of which is too much for the typical biologist or scientist to assemble and comprehend. This is why reviews such as Grasslands and Climate Change, edited by David Gibson and Jonathan Newman, are so valuable. To produce this review of climate change issues and influences relative to grasslands, Gibson and Newman recruited 30 scientists—predominantly from Europe and North America—who wrote 19 chapters dealing with various aspects of grasslands and climate change. The chapters are grouped …

Integration Of Cover Crops Into Midwest Corn-Soybean Cropping Systems And Potential For Weed Suppression, Joshua S. Wehrbein

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Cover crops have potential to provide benefits to agricultural systems, such as improved soil productivity, nutrient scavenging, weed suppression, and livestock forage. There are several challenges associated with cover crop integration into traditional Midwest corn-soybean cropping systems. One of these challenges is timely establishment in the fall, which is limited by the relatively late harvest of corn and soybean. Cover crop effectiveness is related to the amount of biomass produced, thus maximizing the growth period in the fall is desired. To address this challenge, we evaluated the potential to utilize early-season soybean maturity groups (MGs) to allow for earlier soybean …

Benchmarking On-Farm Maize Nitrogen Balance In The Western U. S. Corn Belt, Fatima Amor Tenorio

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A nitrogen (N) balance, calculated as the difference between N inputs and grain-N removal, provides an estimate of the potential N losses. We used N balance with other N-related metrics (partial factor productivity for N inputs, and yield-scaled N balance), to benchmark maize yields in relation with N input use in the US Corn Belt. We first used experimental data on grain-N concentration (GNC) to assess variation in this parameter due to biophysical and management factors. Subsequently, we used N balance and N-related metrics to benchmark yields in relation with N inputs in irrigated and rainfed fields in Nebraska using …

Critical Time For Weed Removal In Corn (Zea Mays L.) As Influenced By Pre Herbicides, Ayse Nur Ulusoy

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

A weed control program that utilizes PRE herbicides and ensures a timely post-emergence weed removal could protect growth and yield of corn. The use of pre-emergence (PRE) herbicides for weed control could reduce the need for multiple POST applications of glyphosate in glyphosate-tolerant (GT) corn and provide an additional mode of action for combating glyphosate-resistant weeds. Thus, field studies were conducted in 2017 and 2018 at Concord, NE with the following objectives develop weed management recommendations that considers soil applied herbicides and determine proper timing of glyphosate based on the crop growth stage.

Therefore the material in this thesis is …

Feasibility Analysis Of Adipic Acid Production, Serpil Unlu

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Adipic acid is an essential intermediate chemical feedstock for the production of nylon-6,6; hence it is valuable from an industrial perspective. Currently, commercial adipic acid production includes a combination of two stages. The first stage produces a mixture of cyclohexanone and cyclohexanol called ‘’KA oil” derived from cyclohexane. In the second stage, KA oil is reacted with nitric acid to produce adipic acid. The second stage generates excess N₂O emission, which is considered as 310 times more potent than CO₂. Renewable feedstocks that can be converted to value-added chemicals offer significant potential for the necessary transition …

Pubfair: A Distributed Framework For Open Publishing Services. Version 2, November 27, 2019, Tony Ross-Hellauer, Benedikt Fecher, Kathleen Shearer, Eloy Rodrigues

Copyright, Fair Use, Scholarly Communication, etc.

Over the last thirty years, digitally-networked technologies have disrupted traditional media, turning business models on their head and changing the conditions for the creation, packaging and distribution of content. Yet, scholarly communication still looks remarkably as it did in the pre-digital age. The primary unit of dissemination remains the research article (or book in some disciplines), and today’s articles still bear a remarkable resemblance to those that populated the pages of Oldenburg’s Philosophical Transactions 350 years ago. In an age of such disruptive innovation, it is striking how little digital technologies have impacted scholarly publishing; and this is also somewhat …

Exposure And Potential Effects Of Pesticides And Pharmaceuticals In Protected Streams Of The Us National Park Service Southeast Region, Paul M. Bradley, Kristin M. Romanok, Jeffrey R. Duncan, William A. Battaglin, Jimmy M. Clark, Michelle L. Hladik, Bradley J. Huffman, Luke R. Iwanowicz, Celeste A. Journey, Kelly L. Smalling

United States National Park Service: Publications

Globally, protected areas offer refugia for a broad range of taxa including threatened and endangered species. In the United States (US), the National Park Service (NPS) manages public lands to preserve biodiversity, but increasing park visitation and development of surrounding landscapes increase exposure to and effects from bioactive contaminants. The risk (exposure and hazard) to NPS protected-stream ecosystems within the highly urbanized southeast region (SER) from bioactive contaminants was assessed in five systems based on 334 pesticide and pharmaceutical analytes in water and 119 pesticides in sediment. Contaminant mixtures were common across all sampled systems, with approximately 24% of the …

Male Burmese Pythons Follow Female Scent Trails And Show Sex-Specific Behaviors, Shannon A. Richard, Eric A. Tillman, John S. Humphrey, Michael L. Avery, M. Rockwell Parker

United States Department of Agriculture Wildlife Services: Staff Publications

Animals communicate with potential mates using species-specific signals, and pheromones are powerful sexual signals that modify conspecific behavior to facilitate mate location. Among the vertebrates, snakes are especially adept in mate searching via chemical trailing, which is particularly relevant given that many snake species are invasive outside their native ranges. Chemical signals used in mate choice are, thus, potentially valuable tools for management of invasive snake species. The Burmese python (Python bivittatus) is an invasive snake in the Florida Everglades where it is negatively impacting native fauna. In this study, we sought to: (i) determine if males can follow conspecific …

Transcriptional Responses Of Soybean Aphids To Sublethal Insecticide Exposure, Laramy S. Enders, Leslie C. Rault, Tiffany Heng-Moss, Blair Siegfried, Nicholas J. Miller

Department of Entomology: Faculty Publications

Insecticides are a key tool in the management of many insect pests of agriculture, including soybean aphids. The selection imposed by insecticide use has often lead to the evolution of resistance by the target pest through enhanced detoxification mechanisms. We hypothesised that exposure of insecticide-susceptible aphids to sublethal doses of insecticides would result in the up-regulation of genes involved in detoxification of insecticides, revealing the genes upon which selection might act in the field. We used the soybean aphid biotype 1 reference genome, version 6.0 as a reference to analyze RNA-Seq data. We identified multiple genes with potential detoxification roles …

Variation In Host Home Range Size Decreases Rabies Vaccination Effectiveness By Increasing The Spatial Spread Of Rabies Virus, Katherine M. Mcclure, Amy T. Gilbert, Richard B. Chipman, Erin E. Rees, Kim M. Pepin

United States Department of Agriculture Wildlife Services: Staff Publications

1. Animal movement influences the spatial spread of directly transmitted wildlife disease through host-host contact structure. Wildlife disease hosts vary in home range- associated foraging and social behaviours, which may increase the spread and intensity of disease outbreaks. The consequences of variation in host home range movement and space use on wildlife disease dynamics are poorly understood, but could help to predict disease spread and determine more effective disease management strategies.
2. We developed a spatially explicit individual-based model to examine the effect of spatiotemporal variation in host home range size on the spatial spread rate, persistence and incidence of rabies virus …

Changes In Students’ Mental Models From Computational Modeling Of Gene Regulatory Networks, Joseph Dauer, Heather E. Bergan-Roller, Gretchen P. King, Mckenzie Kjose, Nicholas J. Galt, Tomáš Helikar

School of Natural Resources: Faculty Publications

Background: Computational modeling is an increasingly common practice for disciplinary experts and therefore necessitates integration into science curricula. Computational models afford an opportunity for students to investigate the dynamics of biological systems, but there is significant gap in our knowledge of how these activities impact student knowledge of the structures, relationships, and dynamics of the system. We investigated how a computational modeling activity affected introductory biology students’ mental models of a prokaryotic gene regulatory system (lac operon) by analyzing conceptual models created before and after the activity.

Results: Students’ pre-lesson conceptual models consisted of provided, system-general structures (e.g., activator, repressor) …

Predicting Agricultural Implement Hydraulic Power Demand Using Synchronized Controller Area Network And Ancillary Sensor Data, Gabriel P. Stoll

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

As agricultural implement designs have progressed in recent years, there has been an increase in hydraulic power demand from the tractor. Current power estimation standards do not accurately estimate hydraulic power demand for implements designed with higher hydraulic power requirements. Several stakeholders, including agricultural producers, tractor and implement manufacturers, and government agencies would benefit from accurate published data on these power requirements.

While an increasing amount of operational data available on the Controller Area Network (CAN) of tractors has assisted researchers in more easily obtaining machinery performance data, hydraulic control valve flow rate and pressure measurements are not currently publically …

Keeping Allergen Names Clear And Defined, Sanny K. Chan, Anna Pomés, Christiane Hilger, Janet M. Davies, Geoffrey Mueller, Annette Kuehn, Andreas L. Lopata, Gabriele Gadermaier, Marianne Van Hage, Monika Raulf, Richard E. Goodman

Department of Food Science and Technology: Faculty Publications

The World Health Organization/International Union of Immunological Societies (WHO/IUIS) Allergen Nomenclature Sub-Committee was established in 1986 by leading allergists to standardize names given to proteins that cause IgE-mediated reactions in humans. The Sub-Committee’s objective is to assign unique names to allergens based on a critical analysis of confidentially submitted biochemical and clinical data from researchers, often prior to publication to preserve consistency. The Sub-Committee maintains and revises the database as the understanding of allergens evolves. This report summarizes recent developments that led to updates in classification of cockroach group 1 and 5 allergens to animal as well as environmental and …

Open Access: Could Defeat Be Snatched From The Jaws Of Victory?, Richard Poynder

Copyright, Fair Use, Scholarly Communication, etc.

When news broke early in 2019 that the University of California had walked away from licensing negotiations with the world’s largest scholarly publisher (Elsevier), a wave of triumphalism spread through the OA Twittersphere. The talks had collapsed because of Elsevier’s failure to offer UC what it demanded: a new-style Big Deal in which the university got access to all of Elsevier’s paywalled content plus OA publishing rights for all UC authors – what UC refers to as a “Read and Publish” agreement. In addition, UC wanted Elsevier to provide this at a reduced cost.1 Given its size and influence, UC’s …

Designation Of A Neotype For Oeneis Melissa (Fabricius, 1775) And A Lectotype For Oeneis Polixenes (Fabricius, 1775) (Lepidoptera: Nymphalidae: Satyrinae), Crispin S. Guppy

The Taxonomic Report of the International Lepidoptera Survey

The Oeneis melissa (Fabricius, 1775) “neotype” designations of Lukhtanov and Eitschberger (2000; 2001) do not meet several critical requirements of the ICZN and are therefore invalid. In addition, the locality data is too vague to be taxonomically useful, their “neotype” specimen is a specimen of the taxon generally known as O. melissa semplei, and acceptance of their specimen as neotype would change the meaning of the taxon Oeneis melissa. Hence, I reject their neotype designation. A neotype Oeneis melissa (Fabricius, 1775) is designated from Nain, Labrador with careful consideration of the Articles of the International Code of Zoological …

Revision Of The Oriental Chaetocnema Species (Coleoptera, Chrysomelidae, Galerucinae, Alticini), Yongying Ruan, Xingke Yang, Alexander S. Konstantinov, Kaniyarikkal Divakaran Prathapan, Mengna Zhang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The Oriental species of Chaetocnema Stephens, 1831 are revised. There are 85 valid species, including 19 new species: C. angustifrons sp. nov.; C. appendiculata sp. nov.; C. baoshanica sp. nov.; C. dapitanica sp. nov.; C. glabra sp. nov.; C. greenica sp. nov.; C. jinxiuensis sp. nov.; C. hongkongensis sp. nov.; C. latapronota sp. nov.; C. midimpunctata sp. nov.; C. nigrilata sp. nov.; C. parafusiformis sp. nov.; C. paragreenica sp. nov.; C. paraumesaoi sp. nov.; C. purerulea sp. nov.; C. reteimpunctata sp. nov.; C. sabahensis sp. nov.; C. subbasalis sp. nov.; C. trapezoida sp. nov.

Fifteen new synonyms are proposed: C. …

Conservation And Management Of Salmon In The Age Of Genomics, Robin S. Waples, Kerry A. Naish, Craig R. Primmer

United States Department of Commerce: Staff Publications

Salmon were among the first nonmodel species for which systematic population genetic studies of natural populations were conducted, often to support management and conservation. The genomics revolution has improved our understanding of the evolutionary ecology of salmon in two major ways: (a) Large increases in the numbers of genetic markers (from dozens to 10⁴– 10⁶) provide greater power for traditional analyses, such as the delineation of population structure, hybridization, and population assignment, and (b) qualitatively new insights that were not possible with traditional genetic methods can be achieved by leveraging detailed information …

Emergence Of Common Lambsquarters (Chenopodium Album L.) Is Influenced By The Landscape Position In Which Seeds Developed, Sharon K. Papiernik, Frank Forcella, Gary B. Amundson

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In a 2-yr field study, we evaluated the emergence and early growth of Chenopodium album L. (common lambsquarters) seedlings as affected by the landscape position in which the seeds (i) developed, (ii) overwintered, and (iii) were planted. Results indicated that a higher proportion of seeds originating from lower slope positions emerged compared with seeds originating from the backslope or upper slope. The timing of emergence was the same for all seed source locations. There was no influence of overwintering location on weed emergence. Regardless of the seed source, we observed faster emergence and growth of C. album planted in the …

Evaluation Of A Global Spring Wheat Panel For Stripe Rust: Resistance Loci Validation And Novel Resources Identification, Ibrahim Elbasyoni, Walid M. El-Orabey, Sabah Morsy, P. S. Baenziger, Zakaria Al Ajlouni4, Ismail M. Dweikat

Department of Agronomy and Horticulture: Faculty Publications

Stripe rust (incited by Puccinia striiformis f. sp. tritici) is airborne wheat (Triticum aestivum L.) disease with dynamic virulence evolution. Thus, anticipatory and continued screening in hotspot regions is crucial to identify new pathotypes and integrate new resistance resources to prevent potential disease epidemics. A global wheat panel consisting of 882 landraces and 912 improved accessions was evaluated in two locations in Egypt during 2016 and 2017. Five prevalent and aggressive pathotypes of stripe rust were used to inoculate the accessions during the two growing seasons and two locations under field conditions. The objectives were to evaluate the panel for …