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Research At Nebraska, 2020-2021 Report, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

This publication is a snapshot of the fiscal year’s activities. To read more stories about research and creativity at Nebraska, visit the online edition at https://research.unl.edu/annualreport/2021. Additional multimedia are available for many stories.

Precise Multispecies Agricultural Gas Flux Determined Using Broadband Open-Path Dual-Comb Spectroscopy, D. I. Herman, C. Weerasekara Et Al.

School of Natural Resources: Documents and Reviews

No abstract provided.

Somatosensory Dysfunction Is Masked By Variable Cognitive Deficits Across Patients On The Alzheimer’S Disease Spectrum, Alex I. Wiesman, Victoria M. Mundorf, Chloe C. Casagrande, Sara L. Wolfson, Craig M. Johnson, Pamela E. May, Daniel L. Murman, Tony W. Wilson

Center for Brain, Biology, and Behavior: Faculty Publications

Background: Alzheimer’s disease (AD) is generally thought to spare primary sensory function; however, such interpretations have drawn from a literature that has rarely taken into account the variable cognitive declines seen in patients with AD. As these cognitive domains are now known to modulate cortical somato-sensory processing, it remains possible that abnormalities in somatosensory function in patients with AD have been suppressed by neuropsychological variability in previous research. Methods: In this study, we combine magnetoencephalographic (MEG) brain imaging during a paired-pulse somatosensory gating task with an extensive battery of neuropsychological tests to investigate the influence of cognitive variability on estimated …

72-H Diurnal Rna-Seq Analysis Of Fully Expanded Third Leaves From Maize, Sorghum, And Foxtail Millet At 3-H Resolution, Xianjun Lai, Claire Bendix, Yan Zhang, James Schnable, Frank G. Harmon

Center for Plant Science Innovation: Faculty Publications

Objectives: The purpose of this data set is to capture the complete diurnal (i.e., daily) transcriptome of fully expanded third leaves from the C4 panacoid grasses sorghum (Sorghum bicolor), maize (Zea mays), and foxtail millet (Setaria italica) with RNA-seq transcriptome profiling. These data are the cornerstone of a larger project that examined the conservation and divergence of gene expression networks within these crop plants. This data set focuses on tem- poral changes in gene expression to identify the network architecture responsible for daily regulation of plant growth and metabolic activities. The power of this data set is fine temporal resolution …

Plasma Membrane Fluidity: An Environment Thermal Detector In Plants, Dora L. Cano-Ramirez, Laura Carmona-Salazar, Francisco Morales-Cedillo, Jorge Ramírez-Salcedo, Edgar B. Cahoon, Marina Gavilanes-Ruíz

Center for Plant Science Innovation: Faculty Publications

The lipid matrix in cell membranes is a dynamic, bidimensional array of amphipathic molecules exhibiting mesomorphism, which contributes to the membrane fluidity changes in response to temperature fluctuation. As sessile organisms, plants must rapidly and accurately respond to environmental thermal variations. However, mechanisms underlying temperature perception in plants are poorly understood. We studied the thermal plasticity of membrane fluidity using three fluorescent probes across a temperature range of -5 to 41 °C in isolated microsomal fraction (MF), vacuolar membrane (VM), and plasma membrane (PM) vesicles from Arabidopsis plants. Results showed that PM were highly fluid and exhibited more phase transitions …

Complete Mitogenomes Of Two Aragoa Species And Phylogeny Of Plantagineae (Plantaginaceae, Lamiales) Using Mitochondrial Genes And The Nuclear Ribosomal Rna Repeat, Jeffrey P. Mower, Lilly Hanley, Kirsten Wolff, Natalia Pabón-Mora, Favio González

Center for Plant Science Innovation: Faculty Publications

Aragoa, comprising 19 high-altitude North Andean species, is one of three genera in the Plantagineae (Plantaginaceae, Lamiales), along with Littorella and Plantago. Based primarily on plastid data and nuclear ITS, Aragoa is sister to a clade of Littorella + Plantago, but Plantagineae relationships have yet to be assessed using multigene datasets from the nuclear and mitochondrial genomes. Here, complete mitogenomes were assembled for two species of Aragoa (A. abietina and A. cleefii). The mitogenomes of both species have a typical suite of genes for 34 proteins, 17 tRNAs, and three rRNAs. The A. abietina mitogenome …

Inmut-Finder: A Software Tool For Insertion Identification In Mutagenesis Using Nanopore Long Reads, Rui Song, Ziyao Wang, Hui Wang, Han Zhang, Xuemeng Wang, Hanh Nguyen, David Holding, Bin Yu, Tom Clemente, Shangang Jia, Chi Zhang

Center for Plant Science Innovation: Faculty Publications

Background: Biological mutagens (such as transposon) with sequences inserted, play a crucial role to link observed phenotype and genotype in reverse genetic studies. For this reason, accurate and efficient software tools for identifying insertion sites based on the analysis of sequencing reads are desired. Results: We developed a bioinformatics tool, a Finder, to identify genome-wide Insertions in Mutagenesis (named as “InMut-Finder”), based on target sequences and flanking sequences from long reads, such as Oxford Nanopore Sequencing. InMut-Finder succeeded in identify > 100 insertion sites in Medicago truncatula and soybean mutants based on sequencing reads of whole-genome DNA or enriched …

3d Reconstruction Identifies Loci Linked To Variation In Angle Of Individual Sorghum Leaves, Michael C. Tross, Mathieu Gaillard, Mackenzie Zwiener, Chenyong Miao, Ryleigh J. Grove, Bosheng Li, Bedrich Benes, James C. Schnable

Center for Plant Science Innovation: Faculty Publications

Selection for yield at high planting density has reshaped the leaf canopy of maize, improving photosynthetic productivity in high density settings. Further optimization of canopy architecture may be possible. However, measuring leaf angles, the widely studied component trait of leaf canopy architecture, by hand is a labor and time intensive process. Here, we use multiple, calibrated, 2D images to reconstruct the 3D geometry of individual sorghum plants using a voxel carving based algorithm. Automatic skeletonization and segmentation of these 3D geometries enable quan- tification of the angle of each leaf for each plant. The resulting measurements are both heritable and …

Somatic Variants For Seed And Fruit Set In Grapevine, Laura Costantini, Paula Moreno-Sanz, Chinedu Charles Nwafor, Silvia Lorenzi, Annarita Marrano, Fabiana Cristofolini, Elena Gottardini, Stefano Raimondi, Paola Ruffa, Ivana Gribaudo, Anna Schneider, Maria Stella Grando

Center for Plant Science Innovation: Faculty Publications

Background: Grapevine reproductive development has direct implications on yield. It also impacts on berry and wine quality by affecting traits like seedlessness, berry and bunch size, cluster compactness and berry skin to pulp ratio. Seasonal fluctuations in yield, fruit composition and wine attributes, which are largely driven by climatic factors, are major challenges for worldwide table grape and wine industry. Accordingly, a better understanding of reproductive processes such as gamete development, fertilization, seed and fruit set is of paramount relevance for managing yield and quality. With the aim of providing new insights into this field, we searched for clones with …

The Crazy Critter Club - Lesson Plans, Caitlin Flynn Copenhaver, Audrey Anderson

Honors Program: Expanded Learning Clubs

This curriculum covers the lesson plans for the 2021 "Cool Critter Club". It is focused on teaching students about animals and the environment, especially at a local level. This club engages students through the use of games, art, and spending time outside.

Validation Of The Short Version Of The Dimensional Inventory For Child Development Assessment, Euclides José De Mendonc¸A Filho, Mônia Aparecida Da Silva, Natalie A. Koziol, Denise Ruschel Bandeira

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

Objectives: There is a critical need to monitor the development of children around the world, and in Brazil, this need is substantial since there is a paucity of assessment tools. This study aimed to describe the design and provide evidence of reliability and validity for the short version of the Dimensional Inventory for Child Development Assessment (IDADI-short). Methods: A sample of 1,865 biological mothers of children aged 4---72 months (M = 34.8, SD = 20.20) completed the IDADI to assess Cognitive, socio-emotional, Expressive, and Receptive Language and Communication, Fine and Gross Motor, and Adaptive Behavior development. The psychometric …

Validation Of The Short Version Of The Dimensional Inventory For Child Development Assessment, Euclides José De Mendonça Filho, Mônia Aparecida Da Silva, Natalie A. Koziol, Denise Ruschel Bandeira

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

Objectives: There is a critical need to monitor the development of children around the world, and in Brazil, this need is substantial since there is a paucity of assessment tools. This study aimed to describe the design and provide evidence of reliability and validity for the short version of the Dimensional Inventory for Child Development Assessment (IDADI-short).

Methods: A sample of 1,865 biological mothers of children aged 4---72 months (M = 34.8, SD = 20.20) completed the IDADI to assess Cognitive, socio-emotional, Expressive, and Receptive Language and Communication, Fine and Gross Motor, and Adaptive Behavior development. The psychometric properties of …

Research And Creative Activity, July 1, 2020-June 30, 2021: Major Sponsored Programs And Faculty Accomplishments In Research And Creative Activity, University Of Nebraska-Lincoln, Office Of Research And Economic Development, University Of Nebraska-Lincoln

Office of Research and Economic Development: Publications

Foreword by Bob Wilhelm, Vice Chancellor for Research and Economic Development, University of Nebraska-Lincoln:

This booklet highlights successes in research, scholarship and creative activity by University of Nebraska–Lincoln faculty during the fiscal year running July 1, 2020, to June 30, 2021.

It lists investigators, project titles and funding sources on major grants and sponsored awards received during the year; fellowships and other recognitions and honors bestowed on our faculty; books and chapters published by faculty; performances, exhibitions and other examples of creative activity; patents and licensing agreements issued; National Science Foundation I-CORPS teams; and peer-reviewed journal articles and conference presentations. …

Magnetism In Curved Geometries, R. Streubel, Evgeny Y. Tsymbal, P. Fischer

Evgeny Tsymbal Publications

No abstract provided.

Timber Talk, Vol. 59, No. 3, September 2021

Timber Talk: Nebraska Forest Industry Newsletter

Timber Talk, Vol. 59, No. 3, September 2021

Timber Talk, Vol. 59, No. 4, December 2021

Timber Talk: Nebraska Forest Industry Newsletter

Timber Talk, Vol. 59, No. 4, December 2021

Timber Talk, Vol. 59, No. 1, March 2021

Timber Talk: Nebraska Forest Industry Newsletter

Timber Talk, Vol. 59, No. 1, March 2021

Fish & Wildlife News: Winter 2021, Matt Trott

Fish and Wildlife News

No abstract provided.

Effects Of Drought On Groundwater-Fed Lake Areas In The Nebraska Sand Hills, N. Shrestha, A. R. Mittelstet, T. E. Gilmore, V. Zlotnik, C. Neale

School of Natural Resources: Faculty Publications

No abstract provided.

Implications Of Historical And Contemporary Processes On Genetic Differentiation Of A Declining Boreal Songbird: The Rusty Blackbird, R. E. Wilson, S. M. Matsuoka, L. L. Powell, J. A. Johnson, D. W. Demarest, D. Stralberg, S. Sonsthagen

School of Natural Resources: Faculty Publications

No abstract provided.

Maneuver Enhancement Brigade, United States Army

United States Army: Field Manuals

Maneuver Enhancement Brigade, United States Army Field Manual FM 3-81

Brigade Combat Team, United States Army

United States Army: Field Manuals

Brigade Combat Team, United States Army Field Manual FM 3-96

Nutech Ventures Annual Report 2020-2021

NUtech Ventures: Publications

NUTech Ventures annual report 2020-2021.

Nutech Ventures Guide To Start-Up Companies At The University Of Nebraska-Lincoln

NUtech Ventures: Publications

NUTech Ventures guide to start-up companies at the University of Nebraska-Lincoln.

Overview

NUtech Ventures is the commercialization affiliate of the University of Nebraska, serving the Lincoln and Kearney campuses. We protect and license the university’s intellectual property and promote entrepreneurship through programming and sponsored events. Ultimately, our mission is to improve quality of life and promote economic development.

NUtech Ventures has created this guide to help aspiring University of Nebraska entrepreneurs in starting their entrepreneurial journey. It is focused on companies formed to commercialize innovations developed at the University of Nebraska–Lincoln and protected via intellectual property rights owned by UNL …

Review: Perspective On High-Performing Dairy Cows And Herds, J. H. Britt, R. A. Cushman, C. D. Dechow, H. Dobson, P. Humblot, M. F. Hutjens, G. A. Jones, F. M. Mitloehner, P. L. Ruegg, I. M. Sheldon, J. S. Stevenson

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Milk and dairy products provide highly sustainable concentrations of essential amino acids and other required nutrients for humans; however, amount of milk currently produced per dairy cow globally is inadequate to meet future needs. Higher performing dairy cows and herds produce more milk with less environmental impact per kg than lower performing cows and herds. In 2018, 15.4% of the world's dairy cows produced 45.4% of the world's dairy cow milk, reflecting the global contribution of high-performing cows and herds. In high-performing herds, genomic evaluations are utilized for multiple trait selection, welfare is monitored by remote sensing, rations are formulated …

U.S. Foreign Policy For The Middle Class: Perspectives From Nebraska, Salman Ahmed, Allison Gelman

Yeutter Institute Publications

U.S. foreign policy has not come up often in the 2020 presidential campaign. But when it has, candidates on both sides of the aisle frequently have stressed that U.S. foreign policy should not only keep the American people safe but also deliver more tangible economic benefits for the country’s middle class. The debate among the presidential contenders is not if that should happen but how to make it happen. All too often, this debate takes place within relatively small circles within Washington, DC, without the benefit of input from state and local officials, small business owners, community leaders, local labor …

Incorporating Animal Movement Into Distance Sampling, R. Glennie, S. T. Buckland, R. Langrock, Tim Gerrodette, Susan Chivers, M. D. Scott

United States Department of Commerce: Staff Publications

Distance sampling is a popular statistical method to estimate the density of wild animal populations. Conventional distance sampling represents animals as fixed points in space that are detected with an unknown probability that depends on the distance between the observer and the animal. Animal movement can cause substantial bias in density estimation. Methods to correct for responsive animal movement exist, but none account for nonresponsive movement independent of the observer. Here, an explicit animal movement model is incorporated into distance sampling, combining distance sampling survey data with animal telemetry data. Detection probability depends on the entire unobserved path the animal …

Managing For Radical Ecosystem Change: Applying The Resist-Accept- Direct (Rad) Framework, Abigail J. Lynch, Laura M. Thompson, Erik A. Beever, David N. Cole, Augustin C. Engman, Cat Hawkins Hoffman, Stephen T. Jackson, Trevor J. Krabbenhoft, David J. Lawrence, Douglas Limpinsel, Robert T. Magill, Tracy A. Melvin, John M. Morton, Robert A. Newman, Jay O. Peterson, Mark T. Porath, Frank J. Rahel, Gregor W. Schuurman, Suresh A. Sethi, Jennifer L. Wilkening

United States Geological Survey: Staff Publications

Ecosystem transformation involves the emergence of persistent ecological or social–ecological systems that diverge, dramatically and irreversibly, from prior ecosystem structure and function. Such transformations are occurring at increasing rates across the planet in response to changes in climate, land use, and other factors. Consequently, a dynamic view of ecosystem processes that accommodates rapid, irreversible change will be critical for effectively conserving fish, wildlife, and other natural resources, and maintaining ecosystem services. However, managing ecosystems toward states with novel structure and function is an inherently unpredictable and difficult task. Managers navigating ecosystem transformation can benefit from considering broader objectives, beyond a …

Ecology Of An Isolated Muskrat Population During Regional Population Declines, Laken S. Ganoe, Matthew J. Lovallo, Justin D. Brown, W. David Walter

United States Geological Survey: Staff Publications

Evidence indicating a decline in Ondatra zibethicus (Muskrat) populations in the United States during the past 40 years has led to speculation regarding factors influencing Muskrat survival. In order to understand population dynamics and survival, it is important to first define the ecology of local populations. We investigated the dwelling structure use, movements, home range, and survival of radio-tagged Muskrats (n = 14) in an urban wetland complex in central Pennsylvania. We used locations collected from intensive radio-telemetry monitoring to determine number of lodging structures used, hourly movement, and size and percent area overlap of home ranges. Muskrats shared …

Elemental And Radionuclide Exposures And Uptakes By Small Rodents, Invertebrates, And Vegetation At Active And Post-Production Uranium Mines In The Grand Canyon Watershed, Danielle Cleveland, Jo Ellen Hinck, Julia S. Lankton

United States Geological Survey: Staff Publications

The effects of breccia pipe uranium mining in the Grand Canyon watershed (Arizona) on ecological and cultural resources are largely unknown. We characterized the exposure of biota to uranium and cooccurring ore body elements during active ore production and at a site where ore production had recently concluded. Our results indicate that biota have taken up uranium and other elements (e.g., arsenic, cadmium, copper, molybdenum, uranium) from exposure to ore and surficial contamination, like blowing dust. Results indicate the potential for prolonged exposure to elements and radionuclides upon conclusion of active ore production. Mean radium-226 in deer mice was up …