Digital Commons Network^™

Exploring Attentional And Emotional Biases As A Function Of Trauma And Dissociation Symptomology, Claudia Clinchard

Department of Psychology: Dissertations, Theses, and Student Research

There is evidence that threatening facial expressions (e.g., angry faces) direct attention toward the target, and that for facial expressions that are less threatening but still convey negative valence (e.g., fear faces) direct attention outward and to one’s environment, therefore causing a shift in memory performance and attentional bias depending on the level of threat in emotional facial expressions presented. Extant literature provides evidence for attentional biases both towards and away from threat in those with posttraumatic stress disorder (PTSD) symptomology. The current study aimed to replicate the previous findings of the effects stimulus and emotion have on memory performance …

Monitoring For Wolves, Jeff Hansen, Cat Urbigkit

Wildlife Damage Management Technical Series

Gray wolves (Canis lupus) and Mexican wolves (Canis lupus baileyi) once again roam across landscapes where they have been absent for decades (Figure 1). With wolf range expansion comes increased opportunities for conflicts when wolves harass or prey on domestic livestock or other animals. Wolves have relatively high reproductive and dispersal rates but detecting individual animals in low-density populations is difficult without a concerted monitoring effort. In fact, wolf presence in an area often is not known until there is a confirmed livestock depredation. Ranchers and wildlife damage management experts need not wait for livestock depredations to occur before wolves …

Toxicity Of Sodium Nitrite-Based Vertebrate Pesticides For European Starlings (Sturnus Vulgaris), Scott J. Werner, Shelagh T. Deliberto, Hailey E. Mclean, Katherine E. Horak, Kirt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

In the 21st century, invasive animals rank second only to habitat destruction as the greatest threat to global biodiversity. Socially-acceptable and cost-effective strategies are needed to reduce the negative economic and environmental impacts of invasive animals. We investigated the potential for sodium nitrite (SN; CAS 7632-00-0) to serve as an avian toxicant for European starlings (Sturnus vulgaris L.). We also assessed the non-target hazard of an experimental formulation of SN that is being developed as a toxicant for invasive wild pigs (Sus scrofa L.). In gavage experiments with European starlings, we identified a lowest observed adverse effect level …

Avian Visual Ecologists Should Consider Uv Absorbance And All Sensory Modalities: A Response To Wisenden Et Al. (2020), Sean T. O'Daniels, Scott J. Werner, Ken Yasukawa

United States Department of Agriculture Wildlife Services: Staff Publications

In a recent publication, Wisenden et al. (2020) examined responses of territorial male Red-winged Blackbirds (Agelaius phoeniceus) to models constructed with ultraviolet (UV)-reflective red epaulets for the purpose of determining if the addition of UV reflectance to epaulets ("UV+") changed the effectiveness of signals to receivers relative to "control" epaulets under field conditions. The authors hypothesized that "UV+epaulet coloration represents a visual signal with increased efficacy in territorial interactions." They presented behavioral data but no visual modeling data. Our aims in this commentary are to suggest alternative terms to those used by the authors, to express concern about the use …

Inhibition Of Zika Virus Replication By G-Quadruplex-Binding Ligands, Indian Institute Of Technology Indore, Aryamav Pattnaik, Bikash R. Sahoo, Uma Shankar, Asit K. Pattnaik, Amit Kumar, Debasis Nayak

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV), a mosquito-transmitted Flavivirus, emerged in the last decade causing serious diseases and affecting human health globally. Currently, no licensed vaccines or antivirals are available to combat ZIKV, although several vaccine candidates are in the pipeline. In recent years, the presence of non-canonical G-quadruplex (GQ) secondary structures in viral genomes has ignited significant attention as potential targets for antiviral strategy. In this study, we identified several novel conserved potential GQ structures by analyzing published ZIKV genome sequences using an in-house algorithm. Biophysical and biochemical analysis of the RNA sequences containing these potential GQ sequences suggested the existence of …

Geographic Distribution: Eleutherodactylus Coqui (Common Coqui). Usa: Florida., Louis A. Somma

Papers in Herpetology

A geographic interception record for Eleutherodactylus coqui in Suwanee County, Florida.

Geographic Distribution: Iguana Iguana (Green Iguana): Usa: Florida., Louis A. Somma, Mark T. Bailey

Papers in Herpetology

A geographic distribution record for Iguana iguana in Marion County, Florida.

Cultivating Hops For Cone Production In Nebraska, Stacy A. Adams

Department of Agronomy and Horticulture: Faculty Publications

The hop cone is the primary product of agronomic value when growing Humulus lupulus L. (common hop). Cones are modified stem and leaf structures that protect the female flower cluster that forms chemical compounds important for flavoring beer and other uses. The majority of hops in the United States (~96%) is grown in the states of Washington, Oregon, and Idaho. Hops can be grown in very diverse climates, but it is the climatic consistency of the Pacific NW that provides product consistency and reasoning commercial hops production is prevalent in the region. Hops is a niche crop outside of the …

3d Printing Of Human Microbiome Constituents To Understand Spatial Relationships And Shape Parameters In Bacteriology, Jacques Izard, Teklu Kuru Gerbaba, Shara R. P. Yumul

Department of Food Science and Technology: Faculty Publications

Effective laboratory and classroom demonstration of microbiome size and shape, diversity, and ecological relationships is hampered by a lack of high-resolution, easy-to-use, readily accessible physical or digital models for use in teaching. Three-dimensional (3D) representations are, overall, more effective in communicating visuospatial information, allowing for a better understanding of concepts not directly observable with the unaided eye. Published morphology descriptions and microscopy images were used as the basis for designing 3D digital models, scaled at 20,000×, using computer-aided design software (CAD) and generating printed models of bacteria on mass-market 3D printers. Sixteen models are presented, including rod-shaped, spiral, flask-like, vibroid, …

Great Expectations: Deconstructing The Process Pathways Underlying Beaver-Related Restoration, Caroline S. Nash, Gordon E. Grant, Susan Charnley, Jason B. Dunham, Hannah Gosnell, Mark B. Hausner, David S. Pilliod, Jimmy Taylor

United States Department of Agriculture Wildlife Services: Staff Publications

Beaver-related restoration is a process-based strategy that seeks to address wide-ranging ecological objectives by reestablishing dam building in degraded stream systems. Although the beaver-related restoration has broad appeal, especially in water-limited systems, its effectiveness is not yet well documented. In this article, we present a process-expectation framework that links beaver-related restoration tactics to commonly expected outcomes by identifying the set of process pathways that must occur to achieve those expected outcomes. We explore the contingency implicit within this framework using social and biophysical data from project and research sites. This analysis reveals that outcomes are often predicated on complex process …

Ecosystem-Scale Biogeochemical Fluxes From Three Bioenergy Crop Candidates: How Energy Sorghum Compares To Maize And Miscanthus, Caitlin E. Moore, Adam C. Von Haden, Mark B. Burnham, Ilsa B. Kantola, Christy D. Gibson, Bethany J. Blakely, Evan C. Dracup, Michael D. Masters, Wendy H. Yang, Evan H. Delucia, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Perennial crops have been the focus of bioenergy research and development for their sustainability benefits associated with high soil carbon (C) and reduced nitrogen (N) requirements. However, perennial crops mature over several years and their sustainability benefits can be negated through land reversion. A photoperiod-sensitive energy sorghum (Sorghum bicolor) may provide an annual crop alternative more ecologically sustainable than maize (Zea mays) that can more easily integrate into crop rotations than perennials, such as miscanthus (Miscanthus × giganteus). This study presents an ecosystem-scale comparison of C, N, water and energy fluxes from energy sorghum, maize and miscanthus during a typical …

Technological Advances To Address Current Issues In Entomology: 2020 Student Debates, Lina Bernaola, Molly Darlington, Kadie Britt, Patricia Prade, Morgan Roth, Adrian Pekarcik, Michelle Boone, Dylan Ricke, Anh Tran, Joanie King, Kelly Carruthers, Morgan Thompson, John J. Ternest, Sarah E. Anderson, Scott W. Gula, Kayleigh C. Hauri, Jacob R. Pecenka, Sajjan Grover, Heena Puri, Surabhi Gupta Vakil

University of Nebraska State Museum: Entomology Papers

The 2020 Student Debates of the Entomological Society of America (ESA) were live-streamed during the Virtual Annual Meeting to debate current, prominent entomological issues of interest to members. The Student Debates Subcommittee of the National ESA Student Affairs Committee coordinated the student efforts throughout the year and hosted the live event. This year, four unbiased introductory speakers provided background for each debate topic while four multi-university teams were each assigned a debate topic under the theme ‘Technological Advances to Address Current Issues in Entomology’. The two debate topics selected were as follows: 1) What is the best taxonomic approach to …

Low Energy Availability With And Without A High-Protein Diet Suppresses Bone Formation And Increases Bone Resorption In Men: A Randomized Controlled Pilot Study, Chaise Murphy, Laura D. Bilek, Karsten Koehler

Department of Nutrition and Health Sciences: Faculty Publications

Suppression of insulin-like growth factor 1 (IGF-1) and leptin secondary to low energy availability (LEA) may contribute to adverse effects on bone health. Whether a high-protein diet attenuates these effects has not been tested. Seven men completed three five-day conditions operationally defined as LEA (15 kcal kg fat-free mass (FFM)^-1 day^-1) with low protein (LEA-LP; 0.8 g protein·kg body weight (BW)^-1), LEA with high protein (LEA-HP; 1.7 g protein·kg BW^-1) and control (CON; 40 kcal·kg FFM^-1·day^-1, 1.7 g protein·kg BW^-1). In all conditions, participants expended 15 kcal·kg …

Especies Nuevas De Lasioglossum Curtis De Las Altas Montañas De Las Antillas Mayores Y Estado Actual Del Conocimiento Del Taxon Habralictellus Moure Y Hurd (Hymenoptera: Apoidea: Halictidae), Julio A. Genaro

Insecta Mundi

Resumen. Se describen para las Antillas cuatro especies nuevas de Lasioglossum Curtis pertenecientes al taxon Habralictellus Moure y Hurd (Hymenoptera: Halictidae): Lasioglossum (Habralictellus) cuba, Lasio­glossum (Habralictellus) darlingtoni, Lasioglossum (Habralictellus) jagibbsi y Lasioglossum (Habralictellus) laupackeri. Se utiliza como subgénero para mantener la estabilidad taxonómica. Se presenta la historia y el estado actual del conocimiento de Habralictellus, que aún no ha encontrado un lugar justificado y aceptado, al unísono por los especialistas, en la nomenclatura zoológica. Se ofrece un listado de las especies conocidas, sexo y distribución.

Abstract. Four new species of Lasioglossum …

A Brief Review Of The New World Xestipyge Marseul (Coleoptera: Histeridae: Dendrophilinae: Paromalini) With Description Of A New Species From The Southwestern Usa, William B. Warner

Insecta Mundi

New World species of the histerid beetle genus Xestipyge Marseul (Coleoptera: Histeridae: Dendrophilinae: Paromalini) are reviewed, and X. skelleyi, new species, is described. A dichotomous key and illustrations are provided as identification means for the now five included species from the Western Hemisphere.

Xestipyge was erected by Marseul (1862) as a monotypic subgenus of his genus Carcinops for “Carcinops radula Marseul” because of its unusual elytral striae, with fourth dorsal stria arched over the fifth dorsal stria and con­necting to the sutural (= sixth dorsal) stria. Lewis elevated Xestipyge to generic status in 1905. In addition to the different …

A New Vaejovis C.L. Koch From The Sierra Madre Occidental Of Durango, Mexico (Scorpiones: Vaejovidae), Jessica S. Azzinnari, Robert W. Bryson, Matthew R. Graham, Carlos Solís-Rojas, W. David Sissom

Insecta Mundi

A new montane species of Vaejovis C.L. Koch (Scorpiones: Vaejovidae) is described from the Si­erra Madre Occidental of Durango, Mexico. Vaejovis baggins Azzinnari, Bryson, Graham, Solís-Rojas, and Sissom, new species, is similar to mexicanus group species in the mountain range, including V. montanus Graham and Bryson, V. sierrae Sissom, Graham, Donaldson, and Bryson, and V. mcwesti Sissom, Graham, Donaldson, and Bryson, but differs from these species based on aspects of body size, pedipalp carination, chelae granulation, morphometrics, and setal patterns on the metasoma. A molecular clock estimate indi­cates that the new species shared a common ancestor with V. mcwesti …

Two New Scarab Beetles From The Southwestern Usa (Coleoptera: Scarabaeidae: Melolonthinae And Aphodiinae), William B. Warner

Insecta Mundi

Phyllophaga (Listrochelus) benwarneri new species and Cinacanthus cunninghami new species (Coleoptera: Scarabaeidae) are described from dune systems in northern Arizona and extreme southern Nevada, USA. Habitus and diagnostic characters of these species are illustrated, and couplets from the respective, most recent (sub)generic keys are modified to incorporate the new species. Available habits and habitat information for each species, and a discussion of the Phyllophaga “senex complex” of species are provided.

This work describes two new scarab beetles from the Southwestern USA, which are apparently restricted to dune systems in northern Arizona to extreme southern Nevada: one, an arenophilic …

The Influence Of Juvenile Dinosaurs On Community Structure And Diversity, Katlin Schroeder, S. Kathleen Lyons, Felisa A. Smith

School of Biological Sciences: Faculty Publications

Despite dominating biodiversity in the Mesozoic, dinosaurs were not speciose. Oviparity constrained even gigantic dinosaurs to less than 15 kg at birth; growth through multiple morphologies led to the consumption of different resources at each stage. Such disparity between neonates and adults could have influenced the structure and diversity of dinosaur communities. Here, we quantified this effect for 43 communities across 136 million years and seven continents. We found that megatheropods (more than 1000 kg) such as tyrannosaurs had specific effects on dinosaur community structure. Although herbivores spanned the body size range, communities with megatheropods lacked carnivores weighing 100 to …

Chilling And Heat Accumulation Of Fruit And Nut Trees And Flower Bud Vulnerability To Early Spring Low Temperatures In New Mexico: Meteorological Approach, Koffi Djaman, Komlan Koudahe, Murali Darapuneni, Suat Irkmak

Department of Mechanical and Materials Engineering: Faculty Publications

Fruit and nut trees production is an important activity across the southwest United States and this production is greatly impacted by the local climate. Temperature is the main environmental factor influencing the growth and the productivity of the fruit and nut trees as it affects the trees’ physiology and the vulnerability of flower bud, flowers, and young fruit and nut to the low temperatures or spring frost. The objective of the present study is to estimate the chilling and heat accumulation of fruit and nut trees across New Mexico. Three study sites as Fabian Garcia, Los Lunas, and Farmington were …

Factors Leading To Successful Island Rodent Eradications Following Initial Failure, Araceli Samaniego, Peter Kappes, Keith Broome, Steve Cranwell, Richard Griffiths, Grant Harper, Pete Mcclelland, Russell Palmer, Gerard Rocamora, Keith Springer, David Will, Shane Siers

United States Department of Agriculture Wildlife Services: Staff Publications

Island rodent eradications are increasingly conducted to eliminate the negative impacts of invasive rodents. The success rate in the tropics has been lower than in temperate regions, triggering research and reviews. Environmental factors unique to the tropics (e.g., land crabs and year-round rodent breeding) have been associated with eradication failure. Operational factors have also been important, but these have not been comprehensively assessed. The environmental and operational factors using global cases where rodent eradication initially failed and subsequent attempts occurred were compared. It was determined whether operational factors explained the initial failures, whether operational improvements explained subsequent successes, and whether …

Structural Basis For Clostridium Perfringens Enterotoxin Targeting Of Claudins At Tight Junctions In Mammalian Gut, Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud

Department of Biochemistry: Faculty Publications

The bacterium Clostridium perfringens causes severe, sometimes lethal gastrointestinal disorders in humans, including enteritis and enterotoxemia. Type F strains produce an enterotoxin (CpE) that causes the third most common foodborne illness in the United States. CpE induces gut breakdown by disrupting barriers at cell–cell contacts called tight junctions (TJs), which are formed and maintained by claudins. Targeted binding of CpE to specific claudins, encoded by its C-terminal domain (cCpE), loosens TJ barriers to trigger molecular leaks between cells. Cytotoxicity results from claudin-bound CpE complexes forming pores in cell membranes. In mammalian tissues, 24 claudins govern TJ barriers—but the basis for …

The Inverse Relationship Between Solar-Induced Fluorescence Yield And Photosynthetic Capacity: Benefits For Field Phenotyping, Peng Fu, Katherine Meacham-Hensold, Matthew H. Siebers, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Improving photosynthesis is considered a promising way to increase crop yield to feed a growing population. Realizing this goal requires non-destructive techniques to quantify photosynthetic variation among crop cultivars. Despite existing remote sensing-based approaches, it remains a question whether solar-induced fluorescence (SIF) can facilitate screening crop cultivars of improved photosynthetic capacity in plant breeding trials. Here we tested a hypothesis that SIF yield rather than SIF had a better relationship with the maximum electron transport rate (Jmax). Time-synchronized hyperspectral images and irradiance spectra of sunlight under clear-sky conditions were combined to estimate SIF and SIF yield, which were then correlated …

Novel And Transgressive Salinity Tolerance In Recombinant Inbred Lines Of Rice Created By Physiological Coupling-Uncoupling And Network Rewiring Effects, Isaiah C.M. Pabuayon, Ai Kitazumi, Kevin R. Cushman, Rakesh Kumar Singh, Glenn B. Gregorio, Balpreet Dhatt, Masoud Zabet-Moghaddam, Harkamal Walia, Benildo G. De Los Reyes

Department of Agronomy and Horticulture: Faculty Publications

The phenomenon of transgressive segregation, where a small minority of recombinants are outliers relative to the range of parental phenotypes, is commonly observed in plant breeding populations. While this phenomenon has been attributed to complementation and epistatic effects, the physiological and developmental synergism involved have not been fully illuminated by the QTL mapping approach alone, especially for stress adaptive traits involving highly complex interactions. By systems-level profiling of the IR29 × Pokkali recombinant inbred population of rice, we addressed the hypothesis that novel salinity tolerance phenotypes are created by reconfigured physiological networks due to positive or negative coupling-uncoupling of developmental …

Epigraph Hemagglutinin Vaccine Induces Broad Cross-Reactive Immunity Against Swine H3 Influenza Virus, Brianna L. Bullard, Brigette N. Corder, Jennifer Debeauchamp, Adam Rubrum, Bette Korber, Richard J. Webby, Eric A. Weaver

School of Biological Sciences: Faculty Publications

Influenza A virus infection in swine impacts the agricultural industry in addition to its zoonotic potential. Here, we utilize epigraph, a computational algorithm, to design a universal swine H3 influenza vaccine. The epigraph hemagglutinin proteins are delivered using an Adenovirus type 5 vector and are compared to a wild type hemagglutinin and the commercial inactivated vaccine, FluSure. In mice, epigraph vaccination leads to significant cross-reactive antibody and T-cell responses against a diverse panel of swH3 isolates. Epigraph vaccination also reduces weight loss and lung viral titers in mice after challenge with three divergent swH3 viruses. Vaccination studies in swine, the …

Identifying Receptors For Neuropeptides And Peptide Hormones: Challenges And Recent Progress, Md Shadman Ridwan Abid, Somayeh Mousavi, James W. Checco

Department of Chemistry: Faculty Publications

Intercellular signaling events mediated by neuropeptides and peptide hormones represent important targets for both basic science and drug discovery. For many bioactive peptides, the protein receptors that transmit information across the receiving cell membrane are not known, severely limiting these signaling pathways as potential therapeutic targets. Identifying the receptor(s) for a given peptide of interest is complicated by several factors. Most notably, cell-cell signaling peptides are generated through dynamic biosynthetic pathways, can act on many different families of receptor proteins, and can participate in complex ligand-receptor interactions that extend beyond a simple one-to-one archetype. Here, we discuss recent methodological advances …

Protease Oma1 Modulates Mitochondrial Bioenergetics And Ultrastructure Through Dynamic Association With Micos Complex, Martonio Ponte Viana, Roman M. Levytskyy, Ruchika Anand, Andreas S. Reichert, Oleh Khalimonchuk

Department of Biochemistry: Faculty Publications

Remodeling of mitochondrial ultrastructure is a process that is critical for organelle physiology and apoptosis. Although the key players in this process—mitochondrial contact site and cristae junction organizing system (MICOS) and Optic Atrophy 1 (OPA1)—have been characterized, the mechanisms behind its regulation remain incompletely defined. Here, we found that in addition to its role in mitochondrial division, metallopeptidase OMA1 is required for the maintenance of intermembrane connectivity through dynamic association with MICOS. This association is independent of OPA1, mediated via the MICOS subunit MIC60, and is important for stability of MICOS and the intermembrane contacts. The OMA1-MICOS relay is required …

Determination Of Vadose Zone And Saturated Zone Nitrate Lag Times Using Long-Term Groundwater Monitoring Data And Statistical Machine Learning, Martin J. Wells, Troy E. Gilmore, Natalie Nelson, John K. Bohlke

Department of Agricultural and Biological Systems Engineering: Faculty Publications

In this study, we explored the use of statistical machine learning and long-term groundwater nitrate monitoring data to estimate vadose zone and saturated zone lag times in an irrigated alluvial agricultural setting. Unlike most previous statistical machine learning studies that sought to predict groundwater nitrate concentrations within aquifers, the focus of this study was to leverage available groundwater nitrate concentrations and other environmental variables to determine mean regional vertical velocities (transport rates) of water and solutes in the vadose zone and saturated zone (3.50 and 3.75 m yr−1 , respectively). The statistical machine learning results are consistent with two primary …

Role Of Nadþ In Regulating Cellular And Metabolic Signaling Pathways, Sara Amjad, Sabah Nisar, Ajaz A. Bhat, Ab Rauf Shah, Michael P. Frenneaux, Khalid Fakhro, Mohammad Haris, Ravinder Reddy, Zoltan Patay, Joseph Baur, Puneet Bagga

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Background: Nicotinamide adenine dinucleotide (NAD⁺), a critical coenzyme present in every living cell, is involved in a myriad of metabolic processes associated with cellular bioenergetics. For this reason, NAD⁺ is often studied in the context of aging, cancer, and neurodegenerative and metabolic disorders.

Scope of review: Cellular NAD⁺ depletion is associated with compromised adaptive cellular stress responses, impaired neuronal plasticity, impaired DNA repair, and cellular senescence. Increasing evidence has shown the efficacy of boosting NAD⁺ levels using NAD⁺ precursors in various diseases. This review provides a comprehensive understanding into the role of NAD^{+ …}

Code Of Best Practices In Fair Use For Open Educational Resources: A Guide For Authors, Adapters & Adopters Of Openly Licensed Teaching And Learning Materials, Meredith Jacob, Peter Jaszi, Prudence S. Adler, William Cross

Copyright, Fair Use, Scholarly Communication, etc.

This code of best practices includes descriptions, hard cases, principles, and considerations for fair uses of materials in open educational resources with respect to United States copyright law, and with some discussion of copyright outside the United States context.

Open Educational Resources and Fair Use

Educators, librarians, and institutions have invested in the creation of openly licensed, freely distributed open educational resources (OER) to advance a wide range of goals within the educational system. Open educational resources enable flexible and open pedagogy; increase access to authorship and facilitate representation of different student experiences; and increase equity by reducing the barriers …

Generation Of A Biobank From Two Adult Thoroughbred Stallions For The Functional Annotation Of Animal Genomes Initiative, Callum G. Donnelly, Rebecca R. Bellone, Erin N. Hales, Annee Nguyen, Scott A. Katzman, Ghislaine A. Dujovne, Kelly E. Knickelbein, Felipe Avila, Ted S. Kalbfleisch, Elena Giulotto, Nicole B. Kingsley, Jocelyn Tanaka, Elizabeth Esdaile, Sichong Peng, Anna Dahlgren, Anna Fuller, Michael J. Mienaltowski, Terje Raudsepp, Verena K. Affolter, Jessica L. Petersen, Carrie J. Finno

Department of Animal Science: Faculty Publications

Following the successful creation of a biobank from two adult Thoroughbred mares, this study aimed to recapitulate sample collection in two adult Thoroughbred stallions as part of the Functional Annotation of the Animal Genome (FAANG) initiative. Both stallions underwent thorough physical, lameness, neurologic, and ophthalmic (including electroretinography) examinations prior to humane euthanasia. Epididymal sperm was recovered from both stallions immediately postmortem and cryopreserved. Aseptically collected full thickness skin biopsies were used to isolate, culture and cryopreserve dermal fibroblasts. Serum, plasma, cerebrospinal fluid, urine, and gastrointestinal content from various locations were collected and cryopreserved. Under guidance of a board-certified veterinary anatomic …