Digital Commons Network^™

Evidence That Toxin Resistance In Poison Birds And Frogs Is Not Rooted In Sodium Channel Mutations And May Rely On “Toxin Sponge” Proteins, Fayal Abderemane-Ali, Nathan D. Rossen, Megan E. Kobiela, Robert A. Craig Ii, Catherine E. Garrison, Zhou Chen, Claire M. Colleran, Lauren A. O'Connell, J Du Bois, John P. Dumbacher, Daniel L. Minor Jr

School of Biological Sciences: Faculty Publications

Many poisonous organisms carry small-molecule toxins that alter voltage-gated sodium channel (Na_V) function. Among these, batrachotoxin (BTX) from Pitohui poison birds and Phyllobates poison frogs stands out because of its lethality and unusual effects on Na_V function. How these toxin-bearing organisms avoid autointoxication remains poorly understood. In poison frogs, a NaV DIVS6 pore-forming helix N-to-T mutation has been proposed as the BTX resistance mechanism. Here, we show that this variant is absent from Pitohui and poison frog NaVs, incurs a strong cost compromising channel function, and fails to produce BTX-resistant channels in poison frog NaVs. We also …

Stockpiling Versus Composting: Effectiveness In Reducing Antibiotic-Resistant Bacteria And Resistance Genes In Beef Cattle Manure, Zachery R. Staley, Bryan L. Woodbury, Bobbi S. Stromer, Amy Schmidt, Daniel D. Snow, Shannon Bartelt-Hunt, Bing Wang, Xu Li

Department of Civil and Environmental Engineering: Faculty Publications

Manure storage methods can affect the concentration and prevalence of antibiotic-resistant bacteria (ARB) and antibiotic resistance genes (ARGs) in cattle manure prior to land application. The objective of this study was to compare stockpiling and composting with respect to their effectiveness in reducing ARB and ARGs in beef cattle manure in a field-scale study. Field experiments were conducted in different seasons with different bulking agents for composting. For both the winter-spring cycle and the summer-fall cycle, ARB concentrations declined below the limit of quantification rapidly in both composting piles and stockpiles; however, ARB prevalence was significantly greater in the composting …

Landscape Of Stress: Tree Mortality Influences Physiological Stress And Survival In A Native Mesocarnivore, Jennifer R. Kordosky, Eric M. Gese, Craig M. Thompson, Patricia A. Terletzky, Lorin A. Neuman-Lee, Jon D. Schneiderman, Kathryn L. Purcell, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Climate change and anthropogenic modifications to the landscape can have both positive and negative effects on an animal. Linking landscape change to physiological stress and fitness of an animal is a fundamental tenet to be examined in applied ecology. Cortisol is a glucocorticoid hormone that can be used to indicate an animal’s physiological stress response. In the Sierra Nevada Mountains of California, fishers (Pekania pennanti) are a threatened mesocarnivore that have been subjected to rapid landscape changes due to anthropogenic modifications and tree mortality related to a 4-year drought. We measured cortisol concentrations in the hair of 64 …

Risks Of Introduction And Economic Consequences Associated With African Swine Fever, Classical Swine Fever And Foot-And-Mouth Disease: A Review Of The Literature, Vienna Brown, Ryan S. Miller, Sophie C. Mckee, Karina H. Ernst, Nicole M. Didero, Rachel M. Maison, Meredith J. Grady, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

African swine fever (ASF), classical swine fever (CSF) and foot-and-mouth disease (FMD) are considered to be three of the most detrimental animal diseases and are currently foreign to the U.S. Emerging and re-emerging pathogens can have tremendous impacts in terms of livestock morbidity and mortality events, production losses, forced trade restrictions, and costs associated with treatment and control. The United States is the world's top producer of beef for domestic and export use and the world's third-largest producer and consumer of pork and pork products; it has also recently been either the world's largest or second largest exporter of pork …

Economic Losses Of Catfish To Avian Predation: A Case Report, Ganesh Kumar, Shraddha Hegde, David Wise, Charles Mischke, Brian Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Avian predation in catfish aquaculture has been a persistent issue throughout the history of the industry, and as production has expanded predation from piscivorous birds has intensified. Catfish ponds in the Mississippi River delta (in the Mississippi migratory flyway) provide a constant and readily accessible supply of forage for birds. Intensive foraging by fish-eating birds has led to a specific regulatory policy and numerous on-farm and regional management efforts. However, in 2016, legal challenges lead to recision of some federal policies and uncertainty as to allowable management, resulting in limitations on bird depredation. Estimating the extent of fish losses to …

Parasites: The Inside Scoop, Scott Lyell Gardner, Judy Diamond, Gábor R. Rácz, Brenda Lee

Zea E-Books Collection

Parasites are organisms that live inside or on another species, called the host. Parasites depend on their hosts for food and a place to live. They may harm the host in small or large ways. Parasitism is the most common mode of life on Earth. Humans, other animals, and all plants have parasites, usually two or more kinds. Even parasites can have parasites. There are millions of species of parasites, and scientists discover new ones every day. Parasite specimens are stored in museums all around the world. One of the world’s largest collections is in the H. W. Manter Laboratory …

Mitochondrial Genome Structure And Double Strand Break Repair In Arabidopsis Thaliana, Sterling Ericsson

School of Biological Sciences: Dissertations, Theses, and Student Research

The mitochondrial genomes of plants are known for their variability in size and arrangement, yet low mutation rates in coding sequences. These opposing characteristics have been suggested to be due to the stability of their DNA maintenance processes and the usage of a double strand break repair (DSBR) system. The components of this repair complex remain poorly understood and only a few of the involved enzymes have been identified, making direct analysis difficult. The flowering plant species Arabidopsis thaliana has been used as a model organism for genome modification and subsequent phenotypic and genotypic impacts. The aims of this research …

Enhancement Of Dry-Aged Beef Quality By Dietary Supplementation Of High Levels Of Vitamin E, David Manuel Velazco Marroquin

Department of Animal Science: Dissertations, Theses, and Student Research

Crossbred cattle (n = 150; 10/pen) were grain-finished with the dietary addition of 2,200 international units of vitamin E (α-tocopherol) per head per day for 100 days. One low-Choice carcass (n = 12) was selected, and strip loins were collected. Low Choice control carcasses (n = 12) were randomly selected from commercial, fed-cattle production to serve as controls. Strip loins were randomly assigned to wet or dry aging for 42 days. On day 42, control dry-aged and control wet-aged loins had the highest TBARS values and vitamin E wet and dry-aged loins had lower TBARS values ( …

The Effect Of Heat Stress And Beta-Adrenergic Agonists On Fatty Acid Mobilization And Their Individual And Interacting Impact On The Adipose Transcriptome Of Ruminant Livestock, Rachel R. Reith

Department of Animal Science: Dissertations, Theses, and Student Research

Heat stress reduces livestock efficiency, induces inflammation, and alters adipose tissue metabolism through stress-induced epinephrine acting on beta-adrenoreceptors (β-AR). Supplementation of beta-adrenergic agonists (β-AA) improve livestock growth and carcass traits, also by acting on β-AR located in skeletal muscle and adipose tissue. The binding of β-AR in adipose tissue results in changes in adipose tissue metabolism including increased lipolysis and decreased fatty acid synthesis. Due to their similar effects on stress response pathways, the possible interaction of heat stress and β-AA could negatively impact animal well-being. The purpose of the first study was to understand how ractopamine (RAC), a beta-one …

Childhood Trauma And Substance Use: Differences By Race And Sex In Juvenile Justice Prevention Programs In Nebraska, Sophie Holtz

Honors Program: Senior Projects (Public)

This study seeks to analyze whether demographic factors such as gender and race have a relationship to the reporting of trauma symptoms in juveniles. This study also examines whether higher substance use has a relationship to higher reports of trauma symptoms. To gather this data, surveys were administered to juveniles involved in juvenile justice prevention programs across the state of Nebraska. Overall, we found that juvenile girls reported significantly higher amounts of trauma symptoms than boys do. There was also a significant difference in how much juvenile girls report using cannabis compared to juvenile boys. Furthermore, there was not a …

Biocomposites Of Low-Density Polyethylene Plus Wood Flour Or Flax Straw: Biodegradation Kinetics Across Three Environments, Anna K. Zykova, Petr V. Pantyukhov, Elena E. Mastalygina, Christian Chaverri-Ramos, Svetlana G. Nikolaeva, Jose J. Saavedra-Arias, Anatoly A. Popov, Sam E. Wortman, Matheus Poletto

Department of Agronomy and Horticulture: Faculty Publications

The purpose of this study was to assess the potential for biocomposite films to biodegrade in diverse climatic environments. Biocomposite films based on polyethylene and 30 wt.% of two lignocellulosic fillers (wood flour or flax straw) of different size fractions were prepared and studied. The developed composite films were characterized by satisfactory mechanical properties that allows the use of these materials for various applications. The biodegradability was evaluated in soil across three environments: laboratory conditions, an open field in Russia, and an open field in Costa Rica. All the samples lost weight and tensile strength during biodegradation tests, which was …

Physical–Chemical Properties, Droplet Size, And Efficacy Of Dicamba Plus Glyphosate Tank Mixture Influenced By Adjuvants, Estefania Gomiero Polli, Guilherme Sousa Alves, Joao Victor De Oliveira, Greg Robert Kruger

Department of Agronomy and Horticulture: Faculty Publications

Dicamba plus glyphosate tank mixture have been largely adopted for postemergence weed control after the development of dicamba-tolerant crops. Ammonium sulfate is commonly used as water conditioner (WC) to increase glyphosate efficacy, but its use is restricted for dicamba herbicides. The use of non-AMS water conditioner and other adjuvants could be a way to optimize efficacy of this tank mixture while mitigating herbicide off-target movement. The objective of this study was to determine the physical–chemical properties and droplet size distribution of dicamba and glyphosate solutions with and without non-AMS WC alone and tank mixed with other adjuvants and evaluate the …

Handling Of Spurious Sequences Affects The Outcome Of High-Throughput 16s Rrna Gene Amplicon Profiling, Sandra Reitmeier, Thomas C. A. Hitch, Nicole Treichel, Nikolaos Fikas, Bela Hausmann, Amanda E. Ramer-Tait, Klaus Neuhaus, David Berry, Dirk Haller, Ilias Lagkouvardos, Thomas Clavel

Department of Food Science and Technology: Faculty Publications

16S rRNA gene amplicon sequencing is a popular approach for studying microbiomes. However, some basic concepts have still not been investigated comprehensively. We studied the occurrence of spurious sequences using defined microbial communities based on data either from the literature or generated in three sequencing facilities and analyzed via both operational taxonomic units (OTUs) and amplicon sequence variants (ASVs) approaches. OTU clustering and singleton removal, a commonly used approach, delivered approximately 50% (mock communities) to 80% (gnotobiotic mice) spurious taxa. The fraction of spurious taxa was generally lower based on ASV analysis, but varied depending on the gene region targeted …

Impact Of Maize Hormonal Interactions On The Performance Of Spodoptera Frugiperda In Plants Infected With Clavibacter Michiganensis Subsp. Nebraskensis, Karen Da Silva, Sydney E. Everhart, Joe Louis

Department of Entomology: Faculty Publications

In nature, plants interact with multiple organisms, below and aboveground. Although interactions of plants with single biotic stressors are well characterized, knowledge of how the immune system responds to multiple biotic stressors is lacking. It is known that the two most important pathways involved in the plant immune defenses are jasmonic acid (JA) and salicylic acid (SA). The crosstalk between these plant hormonal signaling pathways seems to fine-tune the plant responses to different stressors. In this study, we characterized maize (Zea mays) hormonal interactions under the attack of two economically important pests: the bacterial pathogen that causes Goss’s …

Toward Sustainable Production Of Value-Added Bioenergy And Industrial Oils In Oilseed And Biomass Feedstocks, Kiyoul Park, Sanju A. Sanjaya, Truyen Quach, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Plant fatty acids are used forfood, feed, fuel, and industrial materials. Structurally and chemically diverse fatty acids, referred to as unusual or specialized fatty acids, are found in the seed oils of diverse plant species. Many unusual fatty acids have potential use as alternative and renewable sources of biofuels and biobased industrial feedstocks due to their variant structures' physical or functional properties. Oils enriched in these fatty acids can increase the value of oilseed crops and provide co-products that can be readily extracted from lignocellulosic materials in biomass crops. Here, we describe recent progress in strategies for enhancement of oil …

Checklist Of Dermestidae (Insecta: Coleoptera: Bostrichoidea) Of The United States, Jiří Háva, Andreas Herrmann

Insecta Mundi

A checklist of Dermestidae (Coleoptera: Bostrichoidea) of the United States of America is provided. It comprises 171 valid species belonging to 25 different genera, also 6 fossil species and 41 new state records.

Dermestidae is a family of Coleoptera which is commonly referred to as skin beetles. Other common names include larder beetle, hide or leather beetles, carpet beetles, and khapra beetles. There are approximately 1705 species worldwide (Háva 2015a, 2020). The last checklist of the dermestid species occurring in the Nearctic region (Canada and U.S.A.) was published by Beal (2003), with exception of the Hawaiian Islands. However, he had …

How To Distinguish The Xestotrachelus Bruner, 1913 (Orthoptera: Romaleidae: Romaleini) From Other Romaleini In South America, With A Report Of The First Record In Minas Gerais, Brazil, Daniela Santos Martins Silva, Marcelo Ribeiro Pereira, Raysa Martins Lima

Insecta Mundi

The present study provides an important contribution to the knowledge of the geographic distribution of Xestotrachelus Bruner, 1913 (Orthoptera: Romaleidae: Romaleini), a monotypic genus comprised of Xestotrachelus robustus (Bruner, 1911) that has a wide geographic distribution in Brazil. Specimens were collected at the Panga Ecological Reserve, Uberlândia, Minas Gerais, in the Brazilian Cerrado. We provide a key to distinguish Xestotrachelus from other genera found in South America.

Una Nueva Especie De Mosca De Ojos Pedunculados Del Género Richardia Robineau-Desvoidy Y Un Nuevo Reporte De Plagiocephalus Latifrons Wiedemann Para La Costa Pacífica De Colombia (Diptera: Tephritoidea: Richardiidae Y Ulidiidae), Luis Miguel Constantino

Insecta Mundi

En este trabajo se describe una nueva especie de Richardia Robineau-Desvoidy (Diptera: Tephritoidea: Richardiidae), R. chocoensis Constantino, con proyecciones a los lados de la cabeza de forma tubular donde se sitúan los ojos compuestos al final de éstos y con una banda macular característica en las alas que la diferencia de otras especies similares. Adicionalmente se reporta por primera vez a Plagiocephalus latifrons Wiedemann (Diptera: Tephritoidea: Ulidiidae) para el occidente de Colombia. Ambas especies fueron colectadas en las selvas lluviosas de la región biogeográfica del Chocó, en la Costa Pacífica de Colombia.

In this work a new species of Richardia …

Naviauxella Horii, A New Tiger Beetle Species From Myanmar (Coleoptera: Cicindelidae), Jürgen Wiesner, Moe Hnin Phyu

Insecta Mundi

Naviauxella horii Wiesner and Phyu, new species (Coleoptera: Cicindelidae), is described. It is distinguished from the other members of the genus by shape of the aedeagus and shape of the elytral maculation.

Due to the kindness of Michio Hori, we were able to study specimens of a tiger beetle species he recently collected in Myanmar. It proved to be new to science and is described herein. It is a member of the genus Naviauxella Cassola, 1988, which raises the number of species in this genus to 22 (Wiesner 2020: 264).

Emarginoptyx Ballantyne (Coleoptera: Lampyridae: Luciolinae), A Replacement Name For The Firefly Genus Emarginata Ballantyne, 2019, Lesley Ballantyne, Oliver Keller

Insecta Mundi

Emarginata Ballantyne, 2019 (Coleoptera: Lampyridae: Luciolinae), is found to be a junior homonym of the bird genus Emarginata Shelley, 1896 (Avis: Passeriformes: Muscicapidae). The new replacement name Emarginoptyx Ballantyne is provided for the SE Asian genus Emarginata Ballantyne, leading to one new combination, Emarginoptyx trilucida (Jeng, Yang and Lai, 2003).

It has come to our attention that the name Emarginata Ballantyne in Ballantyne et al. (2019) for Emarginata trilucida (Jeng, Yang and Lai, 2003), is preoccupied by Emarginata Shelley, a genus of birds from southern Africa (Shelley 1896).

Luciola Lambkinae Keller And Ballantyne, New Replacement Name For Luciola Roseicollis Pic, 1935 (Coleoptera: Lampyridae), Oliver Keller, Lesley Ballantyne

Insecta Mundi

Two different species descriptions for Luciola roseicollis by Pic (Coleoptera: Lampyridae) are compared, and a replacement name, Luciola lambkinae Keller and Ballantyne nomen novum, for Luciola roseicollis Pic, 1935 is proposed.

In McDermott’s (1966) catalog of the family Lampyridae two citations are listed under the heading Luciola roseicollis Pic, 1933. Both articles include descriptions of L. roseicollis and also the statement that they are new species (Pic 1933, 1935). After comparing both descriptions OK contacted LB with the suspicion that the descriptions are about two different species.

Approaches For And Barriers To The Long-Term Execution Of A Recovery-Oriented Rehabilitation Model Of Treatment In A Community Day Psychiatric Rehabilitation Setting, Sarah Fischer

Department of Psychology: Dissertations, Theses, and Student Research

This dissertation explores the process of creating, implementing, adapting and evaluating a program of care that is composed of specific program features that promote the acquisition of independent living skills, prosocial behavior, progress toward personal recovery goals, and more integrated community living. This program was created and implemented at a community mental health agency that provides integrated mental health care services for individuals with serious mental illness (SMI).

The goals of this dissertation were to (1) compare initial and long-term fidelity to the psychiatric rehabilitation model in order to establish the program’s ability to prevent drift, (2) explore the culture …

Robotic Technologies For High-Throughput Plant Phenotyping: Contemporary Reviews And Future Perspectives, Abbas Atefi, Yufeng Ge, Santosh Pitla, James Schnable

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Phenotyping plants is an essential component of any effort to develop new crop varieties. As plant breeders seek to increase crop productivity and produce more food for the future, the amount of phenotype information they require will also increase. Traditional plant phenotyping relying on manual measurement is laborious, time-consuming, error-prone, and costly. Plant phenotyping robots have emerged as a high-throughput technology to measure morphological, chemical and physiological properties of large number of plants. Several robotic systems have been developed to fulfill different phenotyping missions. In particular, robotic phenotyping has the potential to enable efficient monitoring of changes in plant traits …

Comparison Of Promoter, Dna Vector, And Cationic Carrier For Efficient Transfection Of Hmscs From Multiple Donors And Tissue Sources, Tyler Kozisek, Andrew Hamann, Luke Samuelson, Miguel Fudolig, Angela K. Pannier

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Human mesenchymal stem cells (hMSCs) are primary cells with high clinical relevance that could be enhanced through genetic modification. However, gene delivery, particularly through nonviral routes, is inefficient. To address the shortcomings of nonviral gene delivery to hMSCs, our lab has previously demonstrated that pharmacological “priming” of hMSCs with clinically approved drugs can increase transfection in hMSCs by modulating transfection-induced cytotoxicity. However, even with priming, hMSC transfection remains inefficient for clinical applications. This work takes a complementary approach to addressing the challenges of transfecting hMSCs by systematically investigating key transfection parameters for their effect on transgene expression. Specifically, we investigated …

Critical Time For Weed Removal In Corn As Influenced By Planting Pattern And Pre Herbicides, Dejan Nedeljkovic, Stevan Knezevic, Dragana Bozic, Sava Vrbnicanin

Department of Agronomy and Horticulture: Faculty Publications

Determining the critical time for weed removal (CTWR) is essential for the development of an integrated weed management plan. Therefore, field experiments were conducted to evaluate the effects of two planting patterns (standard and twin-row) with and without PRE-applied herbicides on CTWR in corn. Experiments were laid out in a split-plot arrangement with two main plots: (i) standard row planting (SRP) that is 70 cm wide, and (ii) twin-row planting (TRP) with 50 cm distance between each set of double rows. Each main plot was divided into two sub-plots (with and without PRE herbicides). The sub-sub-plots consisted of seven weed …

Reference Transcriptomes Of Porcine Peripheral Immune Cells Created Through Bulk And Single-Cell Rna Sequencing, Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher Tuggle

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight cell types were isolated in bulk from peripheral blood mononuclear cells (PBMCs) by cell sorting, representing Myeloid, NK cells and specific populations of T and B-cells. Transcriptomes for each bulk population of cells were generated by RNA-seq with 10,974 expressed genes detected. Pairwise comparisons between cell types revealed specific expression, while enrichment analysis identified 1,885 to 3,591 significantly enriched genes across all 8 …

The Brain Transcriptome Of The Wolf Spider, Schizocosa Ocreata, Daniel Stribling, Peter L. Chang, Justin E. Dalton, Christopher A. Conow, Malcolm Rosenthal, Eileen Hebets, Rita M. Graze, Michelle N. Arbeitman

School of Biological Sciences: Faculty Publications

Objectives: Arachnids have fascinating and unique biology, particularly for questions on sex differences and behavior, creating the potential for development of powerful emerging models in this group. Recent advances in genomic techniques have paved the way for a significant increase in the breadth of genomic studies in non-model organisms. One growing area of research is comparative transcriptomics. When phylogenetic relationships to model organisms are known, comparative genomic studies provide context for analysis of homologous genes and pathways. The goal of this study was to lay the groundwork for comparative transcriptomics of sex differences in the brain of wolf spiders, a …

Hybrid Sterility, Genetic Conflict And Complex Speciation: Lessons From The Drosophila Simulans Clade Species, Daven C. Presgraves, Colin D. Meiklejohn

School of Biological Sciences: Faculty Publications

The three fruit fly species of the Drosophila simulans clade— D. simulans, D. mauritiana, and D. sechellia— have served as important models in speciation genetics for over 40 years. These species are reproductively isolated by geography, ecology, sexual signals, postmating-prezygotic interactions, and postzygotic genetic incompatibilities. All pairwise crosses between these species conform to Haldane’s rule, producing fertile F1 hybrid females and sterile F1 hybrid males. The close phylogenetic proximity of the D. simulans clade species to the model organism, D. melanogaster, has empowered genetic analyses of their species differences, including reproductive incompatibilities. But perhaps no phenotype has been subject to …

Can Improved Canopy Light Transmission Ameliorate Loss Of Photosynthetic Efficiency In The Shade An Investigation Of Natural Variation In Sorghum Bicolor, Nikhil S. Jaikumar, Samantha S. Stutz, Samuel B. Fernandes, Andrew D.B. Leakey, Carl J. Bernacchi, Patrick J. Brown, Stephen P. Long

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Previous studies have found that maximum quantum yield of CO2 assimilation (φCO2,max,app) declines in lower canopies of maize and miscanthus, a maladaptive response to self-shading. These observations were limited to single genotypes, leaving it unclear whether the maladaptive shade response is a general property of this C4 grass tribe, the Andropogoneae. We explored the generality of this maladaptation by testing the hypothesis that erect leaf forms (erectophiles), which allow more light into the lower canopy, suffer less of a decline in photosynthetic efficiency than drooping leaf (planophile) forms. On average, φCO2,max,app declined 27% in lower canopy leaves across 35 accessions, …

Creation And Application Of Various Tools For The Reconstruction, Curation, And Analysis Of Genome-Scale Models Of Metabolism, Wheaton L. Schroeder

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Systems biology uses mathematics tools, modeling, and analysis for holistic understanding and design of biological systems, allowing the investigation of metabolism and the generation of actionable hypotheses based on model analyses. Detailed here are several systems biology tools for model reconstruction, curation, analysis, and application through synthetic biology. The first, OptFill, is a holistic (whole model) and conservative (minimizing change) tool to aid in genome-scale model (GSM) reconstructions by filling metabolic gaps caused by lack of system knowledge. This is accomplished through Mixed Integer Linear Programming (MILP), one step of which may also be independently used as an additional curation …