Digital Commons Network^™

Invasive Rodent Responses To Experimental And Natural Hurricanes With Implications For Global Climate Change, Aaron B. Shiels, Gabriela E. Ramírez De Arellano, Laura Shiels

United States Department of Agriculture Wildlife Services: Staff Publications

Hurricanes cause dramatic changes to forests by opening the canopy and depositing debris onto the forest floor. How invasive rodent populations respond to hurricanes is not well understood, but shifts in rodent abundance and foraging may result from scarce fruit and seed resources that follow hurricanes. We conducted studies in a wet tropical forest in Puerto Rico to better understand how experimental (canopy trimming experiment) and natural (Hurricane Maria) hurricane effects alter populations of invasive rodents (Rattus rattus [rats] and Mus musculus [mice]) and their foraging behaviors. To monitor rodent populations, we used tracking tunnels (inked and baited cards …

Improving Efficiency Of Prairie Dog Surveys By Using A Small Copter Drone, Aaron B. Shiels, J. W. Fischer, Danika Spock, Meagan Allira

United States Department of Agriculture Wildlife Services: Staff Publications

Prairie dogs are an accessible and enjoyed wildlife species in Colorado that require occasional surveys because populations can change abruptly due to plague outbreaks or human-induced control. We evaluated the use of small copter drones at four prairie dog colonies on Open Space and Mountain Parks lands, City of Boulder, to determine if this methodology improves efficiency over ground-based survey methods. We counted prairie dogs and burrows using two types of drones (DJI Matric 210 and Autel Evo II) at altitudes 100', 150', and 400' (burrows only). We recorded video and merged still images into orthomosaics prior to having USDA …

Susceptibility Of Wild Canids To Sars-Cov-2, Stephanie M. Porter, Aim E. Hartwig, Helle Bielefeldt-Ohmann, Angela M. Bosco-Lauth, J. Jeffrey Root

United States Department of Agriculture Wildlife Services: Staff Publications

We assessed 2 wild canid species, red foxes (Vulpes vulpes) and coyotes (Canis latrans), for susceptibility to SARS-CoV-2. After experimental inoculation, red foxes became infected and shed infectious virus. Conversely, experimentally challenged coyotes did not become infected; therefore, coyotes are unlikely to be competent hosts for SARS-CoV-2.

Throughout the COVID-19 pandemic, multiple instances of natural infections with SARS-CoV-2 have been reported in pet dogs, likely after exposure to an infected human (1–3). Domestic dogs appear to be minimally susceptible to SARS-CoV-2, as indicated by experimental inoculations resulting in reverse transcription PCR–positive samples and low titer antibody responses but no clinical …

Exploring The Value Of A Global Gene Drive Project Registry, Riley I. Taitingfong, Antoinette J. Piaggio, Cinnamon S. Bloss, Et Al.

United States Department of Agriculture Wildlife Services: Staff Publications

Recent calls to establish a global project registry before releasing any gene-drive-modified organisms (GDOs) have suggested a registry could be valuable to coordinate research, collect data to monitor and evaluate potential ecological impacts, and facilitate transparent communication with community stakeholders and the general public. Here, we report the results of a multidisciplinary expert workshop on GDO registries convened on 8–9 December 2020 involving 70 participants from 14 countries. Participants had expertise in gene drive design, conservation and population modeling, social science, stakeholder engagement, governance and regulation, international policy, and vector control; they represented 45 organizations, spanning national and local governmental …

Determination Of Allergen Levels, Isoforms, And Their Hydroxyproline Modifications Among Peanut Genotypes By Mass Spectrometry, Justin Marsh, Lee K. Palmer, Stef J. Koppelman, Philip E. Johnson

Department of Food Science and Technology: Faculty Publications

The recently published reference genome of peanuts enables a detailed molecular description of the allergenic proteins of the seed. We used LC-MS/MS to investigate peanuts of different genotypes to assess variability and to better describe naturally occurring allergens and isoforms. Using relative quantification by mass spectrometry, minor variation of some allergenic proteins was observed, but total levels of Ara h 1, 2, 3, and 6 were relatively consistent among 20 genotypes. Previously published RPHPLC methodology was used for comparison. The abundance of three Ara h 3 isoforms were variable among the genotypes and contributed to a large proportion of total …

Diversity And Phylogenetic Relationships Of ‘Tetraphyllidean’ Clade 3 (Cestoda) Based On New Material From Orectolobiform Sharks In Australia And Taiwan, Janine N. Caira, Kirsten Jensen

Harold W. Manter Laboratory of Parasitology: Library Materials

In an effort to expand knowledge of Clade 3—one of the ten clades that compose the non-monophyletic order ‘Tetraphyllidea’ all current members of which parasitise orectolobiform sharks—we targeted species of orectolobiform sharks that had not previously been examined for ‘tetraphyllidean’ cestodes. That work led to the discovery of three new species off Australia and Taiwan. Ambitalveolus gen. n. was erected to accommodate these species. Ambitalveolus costelloae gen. n. et sp. n., Ambitalveolus kempi sp. n., and Ambitalveolus penghuensis sp. n. differ from one another in scolex size, genital pore position, and number of marginal loculi, proglottids, and testes. Among ‘tetraphyllideans’, …

Genomic Approaches To Uncovering The Coevolutionary History Of Parasitic Lice [Review], Kevin P. Johnson

Harold W. Manter Laboratory of Parasitology: Library Materials

Next-generation sequencing technologies are revolutionizing the fields of genomics, phylogenetics, and population genetics. These new genomic approaches have been extensively applied to a major group of parasites, the lice (Insecta: Phthiraptera) of birds and mammals. Two louse genomes have been assembled and annotated to date, and these have opened up new resources for the study of louse biology. Whole genome sequencing has been used to assemble large phylogenomic datasets for lice, incorporating sequences of thousands of genes. These datasets have provided highly supported trees at all taxonomic levels, ranging from relationships among the major groups of lice to those among …

Invasion Of Plant Communities, Stephen L. Young, Sarah Kimball, Stephen J. Novak

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Due to numerous human activities, organisms have been transported and either accidentally or deliberately introduced all around the globe. Biological invasions are now considered to be one of the main drivers of global change because many invasive plants have severe ecological, economic, and health consequences. Thus, there is an ever-growing need to better understand invasions to determine how specific plant species are able to establish in communities and, in many cases, expand their range. Here, we describe the invasion process and how it contributes to the invasion of plant communities. We present an invasion-factor framework (IFF) model that uses three …

Predicting Spatial-Temporal Patterns Of Diet Quality And Large Herbivore Performance Using Satellite Time Series, Sean P. Kearney, Lauren M. Porensky, David J. Augustine, Justin D. Derner, Feng Gao

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adaptive management of large herbivores requires an understanding of how spatial-temporal fluctuations in forage biomass and quality influence animal performance. Advances in remote sensing have yielded information about the spatial-temporal dynamics of forage biomass, which in turn have informed rangeland management decisions such as stocking rate and paddock selection for free-ranging cattle. However, less is known about the spatial-temporal patterns of diet quality and their influence on large herbivore performance. This is due to infrequent concurrent ground observations of forage conditions with performance (e.g., mass gain), and previously limited satellite data at fine spatial and temporal scales. We combined multi-temporal …

Predicting Biochemical Acclimation Of Leaf Photosynthesis In Soybean Under In-Field Canopy Warming Using Hyperspectral Reflectance, Etsushi Kumagai, Charles H. Burroughs, Taylor L. Pederson, Christopher M. Montes, Bin Peng, Hyungsuk Kimm, Kaiyu Guan, Elizabeth A. Ainsworth, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Traditional gas exchange measurements are cumbersome, which makes it difficult to capture variation in biochemical parameters, namely the maximum rate of carboxylation measured at a reference temperature (Vcmax25) and the maximum electron transport at a reference temperature (Jmax25), in response to growth temperature over time from days to weeks. Hyperspectral reflectance provides reliable measures of Vcmax25 and Jmax25; however, the capability of this method to capture biochemical acclimations of the two parameters to high growth temperature over time has not been demonstrated. In this study, Vcmax25 and Jmax25 were measured over multiple growth stages during two growing seasons for field-grown …

Comparison Of Stationary And Mobile Canopy Sensing Systems For Irrigation Management Of Maize And Soybean In Nebraska, Sandeep Bhatti, Derek M. Heeren, Susan A. O’Shaughnessy, Steven R. Evett, Mitchell S Maguire, Suresh Pradhyun Kashyap, Christopher M. U. Neale

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Accurate knowledge of plant and field characteristics is crucial for irrigation management. Irrigation can potentially be better managed by utilizing data collected from various sensors installed on different platforms. The accuracy and repeatability of each data source are important considerations when selecting a sensing system suitable for irrigation management. The objective of this study was to compare data from multispectral (red and near-infrared bands) and thermal (long wave thermal infrared band) sensors mounted on different platforms to investigate their comparative usability and accuracy. The different sensor platforms included stationary posts fixed on the ground, the lateral of a center pivot …

Inspiring Young Students To Pursue Engineering, Tami M. Brown-Brandl, Brian Steward

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Imagine elementary school students understanding inputs and outputs, choosing appropriate sensors, and programming an autonomous robot. Imagine middle school students learning about PID control and creating advanced programs for sensor-based decision making. Imagine high school students using CAD to design drive trains, manufacturing parts with CNC machine tools, using finite element analysis to adjust the design, ordering custom components from a machine shop, using image processing for target recognition, and applying path planning and motion control. We have witnessed all of these skills. They happened outside of traditional classrooms, and they were all student-driven.

Yes, it’s true, and it’s amazing. …

Review Of The Duck Factory: A History Of Waterfowl In North Dakota, By Mike Jacobs And Erik Fritzell, Douglas Johnson

The Prairie Naturalist

It was by geological accident that North Dakota became the heart of duck production in the continental United States. When the Wisconsin glaciation retreated some 11,000 years ago, it left an uneven layer of glacial till over much of North Dakota and nearby states and provinces, an area termed the Prairie Pothole Region. Large blocks of ice in the glacial till eventually melted and formed depressions that became wetlands, or prairie potholes. These were embedded in a matrix of soil that, with millennia of growth by deep-rooted prairie plants, became rich topsoil. The wetlands, surrounded by luxuriant grassland, were ideal …

Review Of Loon Lessons: Uncommon Encounters With The Great Northern Diver, By James D. Paruk, Kristin Bianchini

The Prairie Naturalist

Gavia immer Brunnich (Common Loon) is a bird that has captivated bird watchers, wildlife enthusiasts, campers, cottagers, and others interested in the natural world for centuries. The loon also features prominently in Native American mythology. For many, the loon symbolizes the wildness of the north, and it is often used as a sentinel of lake health. Indeed, the Common Loon has inspired a wealth of literature, both scientific and popular, describing this species’ ecology, behavior, and fitness in the face of environmental changes. To this literature, Loon Lessons: Uncommon Encounters with the Great Northern Diver adds a complete and accurate …

Aspergillus Nidulans Septa Are Indispensable For Surviving Cell Wall Stress, Ryland N. Spence, Walker Huso, Harley Edwards, Alexander Doan, Samantha Reese, Steven D. Harris, Ranjan Srivastava, Mark R. Marten

School of Biological Sciences: Faculty Publications

Septation in filamentous fungi is a normal part of development, which involves the formation of cross-hyphal bulkheads, typically containing pores, allowing cytoplasmic streaming between compartments. Based on previous findings regarding septa and cell wall stress, we hypothesized that septa are critical for survival during cell wall stress. To test this hypothesis, we used known Aspergillus nidulans septation-deficient mutants (ΔsepH, Δbud3, Δbud4, and Δrho4) and six antifungal compounds. Three of these compounds (micafungin, Congo red, and calcofluor white) are known cell wall stressors which activate the cell wall integrity signaling pathway (CWIS), while the three others (cycloheximide, miconazole, and 2,3-butanedione monoxime) …

Course Portfolio For Bioc934: Genome Dynamics And Gene Expression, Nicole R. Buan

UNL Faculty Course Portfolios

A graduate degree in Biochemistry or any molecular life science field requires a strong foundation in molecular biology. BIOC934 Genome Dynamics and Gene Expression is a course designed to challenge graduate students whose research interests lie in the areas of biochemistry, molecular biology, synthetic biology, bioinformatics, and systems biology of prokaryote and eukaryote systems. Graduate students in these disciplines require a rigorous and thorough examination of core concepts in addition to practice applying theory to new observations while traversing unfamiliar disciplinary terrain. This portfolio describes the design of BIOC934, its implementation, and observed outcomes to identify strengths of the course …

Interaction With Augmented Reality Sandbox Does Not Produce Greater Gains In Topographic Map Skills For Undergraduate Students, Celeste Kenworthy

Honors Program: Senior Projects (Public)

The augmented reality (AR) sandbox allows students to interact with topographic maps in a 3D space. Being able to understand topographic maps is important to geologists and they are taught in many introductory geology courses. Recent research has focused on whether the AR sandbox can improve students’ topographic map skills. Previous studies have found that students who interact with the AR sandbox do not score significantly better on topographic map assessments (TMAs) than their peers. One proposed reason for this is the limited time students have to interact with the AR sandbox. This study sought to address this by creating …

Can Gdp Be Predictive Of Fertility Rates In Sweden?, Hannah Coleman

Honors Program: Senior Projects (Public)

Fertility rates in a country vary and can be influenced by numerous different aspects of an individual’s life and the society in which they live. One aspect that may influence fertility rates at the national level is the gross domestic product (GDP). GDP is an indicator of a country’s economic productivity. Because fertility is linked to both economic development and individual resources, GDP may be a useful indicator of fertility rates. If so, knowing or predicting GDP could be used to project the fertility rates of a population years into the future. To determine if a reasonable connection between GDP …

Optimization And Standardization Of Dms Chemical Mapping Experiments For Probing Rna 3d Structure, Sarah Brady

Honors Program: Senior Projects (Public)

RNA plays a significant and crucial role in biological processes. The properties of RNA are ultimately responsible for each molecule’s unique function. The configuration and structural integrity of RNA is important for its functionality. Chemical mapping detailed in this thesis can be used to analyze the 3D structure of an RNA sequence. RNA is modified via methylation of adenine and cytosine bases using dimethyl sulfate, then reverse transcribed to DNA, then sequenced. The sites where the bases were methylated become mutations in the DNA sequence, allowing for identification of these locations when analyzing sequencing data. Conditions for modification can be …

Seek, Picture Insect, Google Lens: An Analysis Of Popular Insect Identification Apps Using Photos Of Realistic Quality, Morgan Manderfield

Department of Entomology: Distance Master of Science Projects

Photo-based identification apps for mobile devices have grown in popularity across many fields of organismal biology. While studies have shown that these apps show promise in fields such as botany and ornithology, there is much skepticism surrounding their accuracy and usefulness in entomology. While providing these apps with photos of high quality will undoubtedly lead to the highest accuracy rates, often users submit photos of average quality, e.g., in poor lighting, out of focus, from far away, etc. To quantify realistic accuracy rates of photo-based insect identification apps, this study analyzed three popular apps – Seek, Picture Insect, and Google …

“One Bug, Two Bug, Good Bug, Bad Bug”, Ivy Grob

Department of Entomology: Distance Master of Science Projects

Integrated Pest Management (IPM) is a holistic strategy for the management of pest species. It is a framework that relies on scientifically informed decisions to identify and remediate a pest problem in a way that minimizes harm to the environment, human health, and the economy (Dara, 2019). The term pest is broadly defined not just as a nuisance, but as any organism that has detrimental effects to a resource or public health and that exists at a population level that exceeds a threshold of economic damage. This encompasses invertebrates (such as insects, nematodes, and mites), vertebrates, plant pathogens, diseases, and …

A Guide To Agricultural Insect Pests On The East Coast, Devan Mazza-Mcdonald

Department of Entomology: Distance Master of Science Projects

A Guide to Agricultural Insect Pests on the East Coast is a useful resource comprised of fact sheets of ten invasive pests that have been introduced to the United States. It also includes a fact sheet of three important pollinator species that are of economic and environmental importance. Most of the pests I selected are currently an issue in states on the East coast and in some instances, further west as well. Currently, the khapra beetle is not a problem pest in the United States but does have a possibility of becoming an issue, again. The invasive pests I chose …

Promising Findings That The Cultivating Healthy Intentional Mindful Educators’ Program (Chime) Strengthens Early Childhood Teachers’ Emotional Resources: An Iterative Study, Holly Hatton-Bowers, Caron Clark, Gilbert R. Parra, Jessica L. Calvi, Michael Yellow Bird, Pearl Avari, Jaclynn Foged, John Smith

Department of Child, Youth, and Family Studies: Faculty Publications

Findings suggest that an eight-week mindfulness compassion-based program, Cultivating Healthy Intentional Mindful Educators (CHIME), is a feasible professional development intervention for early childhood (EC) teachers to support their emotion regulation and psychological and workplace well-being. We offer preliminary evidence that learning about mindfulness, self-compassion, and social-emotional learning supports EC teachers in strengthening their knowledge and application of practices to be more mindful and less emotionally reactive and emotionally exhausted at work. In analyzing both EC teacher feedback and survey data from two pilot studies, there was promising evidence that participating in CHIME enhanced awareness of emotions and the development of …

Investigating Moderators Of Daily Marital To Parent–Child Spillover: Individual And Family Systems Approaches, Patty X. Kuo, Kejin Lee, Victoria J. Johnson, Emily J. Starr

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: We tested whether cognitive reappraisal and coparenting quality moderate marital to parent–child spillover in mothers and fathers.

Background: The influence of marital relationship quality on parent–child relationships, referred to as the spillover effect, is well documented. Factors that may attenuate the occurrence of spillover, however, remain unclear. Cognitive reappraisal, an emotion regulation strategy that promotes the reframing of emotional situations as neutral or positive, and coparenting—the intermediate subsystem between the marital and parent–child relationships—may buffer the effects of marital to parent–child spillover.

Method: Using daily diary data from mother–father couples (N = 96) of young children (M_age = …

A Pivotal New Approach To Groundwater Quality Assessment, M. L. Cherry, T. Gilmore, T. Messer, Y. Li, J. Westrop

School of Natural Resources: Faculty Publications

No abstract provided.

Natural Environment: Protocol To Recover Pollen Grains On Bodies, Karl J. Reinhard, A. Barbosa

School of Natural Resources: Faculty Publications

No abstract provided.

Reevaluation Of The Described Subspecies Of Euphydryas Phaeton (Drury, 1773) With A Replacement Name For Melitaea Phaeton Schausi (Clark, 1927), Harry Pavulaan

The Taxonomic Report of the International Lepidoptera Survey

The present paper reevaluates the subspecific standing of the nominotypical and three described subspecies of Euphydryas phaeton (Drury, 1773). The nominotypical subspecies phaeton occupies the mid-Atlantic region, with undefined zones of contact with described subspecies borealis (F. Chermock & R. Chermock, 1940) to the north, and subspecies schausi (Clark, 1927) to the south. Nominotypical phaeton is an intermediate phenotype between borealis and schausi, which are each noticeably different from each other but both reasonably similar to intermediate (nominotypical) phaeton. Both borealis and schausi were synonymized under nominotypical phaeton since about time of their descriptions, by authors and list …

Occurrence Of An Exotic Whitefly, Siphoninus Phillyreae (Haliday) (Hemiptera: Aleyrodidae) In South Korea And Its Potential Pathway Analysis, Seon Woo Lee, Hyun Jik Lee, Jun-Ran Kim, Soo-Jung Suh

Insecta Mundi

The ash whitefly, Siphoninus phillyreae (Haliday) (Hemiptera: Aleyrodidae), was first found in southern areas of South Korea during September 2019. During the survey of 2021, no additional specimens of the ash whitefly were found other than the previously reported regions. Attempts to trace a possible path­way for exotic ash whitefly suggested that this species probably followed pathways of illegal importation of infested plants from China or Japan and was unintentionally introduced into South Korea based on a survey-based study. This whitefly has not been intercepted at ports of entry to South Korea on imported plant material between 1999 and 2019 …

New Synonymies And Notes In Criodion Audinet-Serville (Coleoptera: Cerambycidae: Cerambycinae), Antonio Santos-Silva, Sergio Devesa

Insecta Mundi

Criodion angustatum Buquet, 1852 and C. pilosum Lucas, 1857 (Coleoptera: Cerambycidae: Cerambycinae) are synonymized with C. tomentosum Audinet-Serville, 1834. The holotypes of C. angustatum and C. hirsutum, and syntypes of C. pilosum and of C. tuberculatum Gahan, 1892 are illustrated for the first time. One of the type localities of C. tuberculatum is corrected.

Currently, Criodion Audinet-Serville, 1834 includes 13 species distributed from Guatemala to southern South America (Monné 2021; Tavakilian and Chevillotte 2021). Despite the relatively small number of species, they encompass several problems with their identification. For example, the specimens often identified as C. murinum Nonfried, 1895 …

Nesting Behavior, Ecology, And Functional Morphology Of The Trapdoor Spider-Hunting Spider Wasp Aporus (Plectraporus) Hirsutus (Banks) (Hymenoptera: Pompilidae), Frank E. Kurczewski, Alice J. Abela, Rick C. West

Insecta Mundi

Macrophotographs in series taken by Alice Abela on sandy coastal dunes in Santa Barbara and San Luis Obispo Counties, CA in 2010–2021 supplement and enhance F. X. Williams (1928) study of the ecol­ogy and nesting behavior of the trapdoor spider-hunting spider wasp Aporus (Plectraporus) hirsutus (Banks) (Hymenoptera: Pompilidae: Aporini). Abela’s macrophotographs and observations provide new details of adult wasp feeding, functional morphology, hunting, digging and prey transport, and host spider trapdoor, entrance, burrow structure, host capture and escape activity. Newly reported host records from this study and online photographs expand A. hirsutus host selection in the large wafer-lid …