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Roles Of Dnak And Rpos In Starvation-Induced Thermotolerance Of Escherichia Coli, David Rockabrand, Kevin Livers, Tess Austin, Robyn Kaiser, Debra Jensen, Richard Burgess, Paul H. Blum

School of Biological Sciences: Faculty Publications

DnaK is essential for starvation-induced resistance to heat, oxidation, and reductive division in Escherichia coli. Studies reported here indicate that DnaK is also required for starvation-induced osmotolerance, catalase activity, and the production of the RpoS-controlled Dps (PexB) protein. Because these dnaK mutant phenotypes closely resemble those of rpoS (o³⁸) mutants, the relationship between DnaK and RpoS was evaluated directly during growth and starvation at 30°C in strains with genetically altered DnaK content. A starvation-specific effect of DnaK on RpoS abundance was observed. During carbon starvation, DnaK deficiency reduced RpoS levels threefold, while DnaK excess increased RpoS levels …

Molecular Characterization Of The Α-Glucosidase Gene (Mala) From The Hyperthermophilic Archaeon Sulfolobus Solfataricus, Michael Rolfsmeier, Cynthia Haseltine, Elisabetta Bini, Amy Clark, Paul H. Blum

School of Biological Sciences: Faculty Publications

Acidic hot springs are colonized by a diversity of hyperthermophilic organisms requiring extremes of temperature and pH for growth. To clarify how carbohydrates are consumed in such locations, the structural gene (malA) encoding the major soluble α-glucosidase (maltase) and flanking sequences from Sulfolobus solfataricus were cloned and characterized. This is the first report of an α-glucosidase gene from the archaeal domain. malA is 2,083 bp and encodes a protein of 693 amino acids with a calculated mass of 80.5 kDa. It is flanked on the 5’ side by an unusual 1-kb intergenic region. Northern blot analysis of the …

Population Growth Of Rhinocyllus Conicus (Coleoptera: Curculionidae) On Two Species Of Native Thistles In Prairie, Svata M. Louda

Svata M. Louda Publications

The insect Rhinocyllus conicus Froehlich is a flowerhead weevil deliberately introduced into the United States for the biological control of invasive exotic thistles in the genus Carduus. This study documents the course and magnitude of the weevil population expansion onto nontarget host plants. No weevils were reared from either Platte thistle, Cirsium canescens Nuttall, or wavyleaf thistle, C. undulatum (Nuttall) Sprengel, from 1977 to 1993 at 2 Sandhills prairie preserves: Arapaho Prairie and Niobrara Valley. For Platte thistle, the number of both R. conicus adults on plants and the number of larvae developing in flowerheads increased significantly from 1993 …

Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor

Papers in Microbiology

A 70-kDa glycoprotein, gp70, was found enriched in the detergentinsoluble cytoskeletal fraction of axenically grown Dictyostelium discoideum cells. Its N-terminal amino acid sequence identified it as “crystal protein” (L. Bomblies et al., 1990, J. Cell Biol. 110, 669– 679). This finding was corroborated when antibody to crystal protein cross-reacted with gp70 and its deglycosylated form. The postulated esterase activity of gp70/crystal protein was verified through comparative enzyme assays of extracts derived from cells that either overexpressed or lacked gp70. Gp70 cosedimented with cytoskeletons on sucrose gradients, suggesting an interaction with the cytoskeleton. Coisolation of gp70 with detergent-extracted cells, observed by …

Exercise Capacity Of House Wren Nestlings: Chicks Are Not Working As Hard As They Can, Mark A. Chappell, Gwendolyn C. Bachman

Papers in Behavior in Biological Sciences

We measured the aerobic capacity for exercise in house wren (Troglodytes aedon) nestlings from 3 to 10 days of age. Exercise data were compared with previous measurements of the energy cost of begging (E_beg) to determine if begging chicks are working at maximal exercise capacity. We also compared exercise with the peak metabolic rate during digestion and, in older chicks, with thermogenic heat production. Rates of oxygen consumption (VO₂) during exercise increased rapidly with age, but the factorial aerobic scope for exercise (exercise VO₂/resting VO₂) averaged only 1.4 (at three …

The Effect Of Background Cuing On Prey Detection, Henry Kono, Pamela J. Reid, Alan Kamil

Papers in Behavior in Biological Sciences

Studies of prey detection have typically focused on how search image affects the capture of cryptic items. This study also considers how background vegetation influences cryptic prey detection. Blue jays, Cyanocitta cristata, searched digitized images for two Catocala moths: C. ilia, which is cryptic on oak, and C. relicta, which is cryptic on birch. Some images contained moths while others did not. The ability of blue jays to detect prey during repeated presentations of one prey type within a session was compared with their performance during randomly alternating presentations of both prey types within a session to …

Metazoan Parasites And Other Symbionts Of Cetaceans In The Caribbean, Antonio A. Mignucci-Giannoni, Eric P. Hoberg, Doug Siegel-Causey, Ernest H. Williams Jr.

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The parasite fauna in cetaceans from Puerto Rico, the Virgin Islands, and the larger Caribbean region is poorly known. We provide the first records for parasite biodiversity among a diverse assemblage of cetaceans from the Caribbean Sea. Internal and external parasites and commensals were collected from stranded whales and dolphins salvaged in Puerto Rico, the United States and British Virgin Islands, Jamaica, and Venezuela between 1989 and 1997. A total of 47 individuals of 16 species of whales and dolphins (15 odontocetes and one mysticete) were examined. Overall, parasites and commensals were found in 34 (72.3%) animals, representing 13 species …

The Identification And Characteristics Of Echinoparyphium Rubrum (Cort, 1914) Comb. New (Trematoda, Echinostomatidae) Based On Experimental Evidence Of The Life Cycle, Ivan Kanev, Robert Sorensen, Mauritz Strerner, Rebecca Cole, Bernard Fried

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The life cycle of Echinoparyphium rubrum (Cort, 1914) comb. n. has been completed experimentally. All of the developmental stages - egg, miracidium, sporocyst, mother and daughter rediae, cercaria, metacercaria, and adult - were examined and described. The miracidia infected freshwater snails of the genus Physa, P. gyrina and P. occidentalis. Attempts to infect snails of the genera Lymnaea, L. auricularis, L. peregra. L. truncatula and Bulinus, B. truncatus failed. Cercariae infected various pulmonate and prosobranch freshwater snails, mussels, frogs, water turtles and planarians. The adults developed in the small intestine of birds and mammals. The identity and major characteristics of …

Metathesis-Based Synthesis Of Jasmonate And Homojasmonate Lactones, Candidates For Extracellular Quorum Sensing Molecules In Candida Albicans, Su C. Cho, Patrick Dussault, Amber D. Lisec, Ellen C. Jensen, Kenneth W. Nickerson

Department of Chemistry: Faculty Publications

Ring-closing metathesis of jasmonate esters is shown to provide a rapid entry to jasmonate lactones. The lactones were investigated as possible quorum sensing molecules for the fungi Candida albicans. Assays demonstrated no effect on fungal morphology at concentrations up to 96 mM.

Biodiesel Fuel From Animal Fat. Ancillary Studies On Transesterification Of Beef Tallow, Fangrui Ma, L. Davis Clements, Milford Hanna

Nebraska Center for Biotechnology: Faculty and Staff Publications

Transesterification of beef tallow was investigated. The solubility of ethanol in beef tallow was much higher than that of methanol. At 100 °C the solubility of methanol was 19% (w/w). The solubility of ethanol in beef tallow reached 100% (w/w) at about 68 °C. For the distribution of methanol between beef tallow methyl esters (BTME) and glycerol, the percentage of total methanol in the glycerol phase was higher than that in the fatty acid methyl ester (FAME) phase in a simulated system at room temperature. At 65-80 °C, however, the percentage of total methanol in FAME (60% (w/w)) was higher …

Mummies And Mummification Practices In The Southern And Southwestern United States, Mahmoud Y. El-Najjar, Thomas M. J. Mulinski, Karl Reinhard

Karl Reinhard Publications

Mummification was not intentional for most North American prehistoric cultures. Natural mummification occurred in the dry areas of North America, where mummies have been recovered from rock shelters, caves, and overhangs. In these places, corpses desiccated and spontaneously mummified. In North America, mummies are recovered from four main regions: the southern and southwestern United States, the Aleutian Islands, and the Ozark Mountains of Arkansas. This chapter is limited to a discussion of burial practices and a tabulation of the location of mummies in the southwestern United States with some comments on mummies of the southern United States, including the Ozarks.

New Investigative Techniques [In Mummies, Disease & Ancient Cultures], Theodore A. Reyman, Henrick Nielsen, Ingolf Thuesen, Derek N. H. Notman, Karl Reinhard, Edmund Tapp, Tony Waldron

Karl Reinhard Publications

In this chapter, we shall see how the amplification of minute amounts of genetic material by the polymerase chain reaction (PCR) has enabled researchers to identifY both native and infectious DNA in these bodies. The use of amplified bacterial and viral DNA may turn out to be Aidan Cockburn's 'magic bullet' for solving some of the mysteries of infectious disorders in ancient populations. In the same fashion, native DNA may give us clues to a variety of genetic abnormalities that existed in those distant times. These newer techniques will be the focus of the revised technical section in this second …

Human Dental Microwear Caused By Calcium Oxalate Phytoliths In Prehistoric Diet Of The Lower Pecos Region, Texas, Dennis Danielson, Karl Reinhard

Karl Reinhard Publications

Recent research demonstrates that silica phytoliths of dietary origin are associated with microwear of human teeth. Previous research has shown that severe enamel microwear and dental wear characterizes Archaic hunter-gatherers in the lower Pecos region of west Texas. Calcium oxalate crystals are especially common in Archaic coprolites. The vast majority are derived from prickly pear and agave, which were the dietary staples in west Texas for 6,000 years. The calcium oxalate phytoliths are harder than enamel. Therefore, calcium oxalate crystals are the most likely source of previously documented dental microwear and wear in the lower Pecos region.Am J Phys Anthropol …

Book Review Of Paleolimnology Of Europeanmaar Lakes By J. F.W. Negendank & B. Zolitschka (Eds)., J. Platt Bradbury

United States Geological Survey: Staff Publications

This book represents a collection of papers presented at a symposium in May, 1991 on the formation and paleolimnology of European maar lakes. Paleolimnological discussions embrace maar lake deposits of late Quaternary to early Tertiary age in Germany, France and Italy. The goal of the symposium and its outgrowth, this book, is to develop a funding initiative to study, core and analyze the deposits of these remarkable depositional systems.

Early Life History Stages Of Gulf Sturgeon In The Suwannee River, Florida, Kenneth J. Sulak, James P. Clugston

United States Geological Survey: Staff Publications

Egg sampling confirmed that Suwannee River Gulf sturgeon Acipenser ozyrinchus desotoi, a subspecies of Atlantic sturgeon A. o. oxyrinchus use the same spawning site at river kilometer (rkm) 215 from the mouth of the river each year. Forty-nine eggs were recorded in 1995, and 368 were recorded in 1996. Spawning began 4-7 d after the March new moon in both years and lasted 10-11 d; in 1996, a second 10-d spawning round began on the April new moon. Developmental synchrony among eggs recovered suggested serval discrete spawning events in both years. Total eggs deposited for three 1996 sampling days …

Atrazine, Deethylatrazine, And Deisopropylatrazine Persistence Measured In Groundwater In Situ Under Low-Oxygen Conditions, Sharon Papiernik, Roy Spalding

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The degradation of atrazine [2-chloro-4-(ethylamino)-6-isopropylamino-1,3,5-triazine], deethylatrazine [DEA; 2-amino-4-chloro-6-(isopropylamino)-1,3,5-triazine], and deisopropylatrazine [DIA; 2-amino-4-chloro-6-(ethylamino)-1,3,5-triazine] was assessed under limited oxygen conditions using in situ microcosms. Denitrification was induced in a shallow sand and gravel aquifer to measure the potential for degradation of atrazine, DEA, and DIA under low-O2 conditions. The dissolved oxygen content decreased from 7-8 mg/L to ≤1 mg/L within 4 days and remained ≤ mg/L for the remainder of the 45-day experiment. Atrazine, DEA, and DIA concentrations (normalized to the bromide concentration at each sampling time to account for dilution) did not show a significant decrease with time, indicating that these …

Feasibility Of Aeration For Management Of Maize Weevil Populations In Corn Stored In The Southern United States: Model Simulations Based On Recorded Weather Data, Frank H. Arthur, James E. Throne, Dirk E. Maier, Michael D. Montross

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Recorded data from weather stations in 11 southern states were used to determine the optimum initial activation temperatures for immediate cooling of corn that is stored after harvest. Hours of temperature accumulation in September and October that were below 12.8 and 15.6°C (55 and 60 OF),levels that often are recommended for fall cooling of hard red winter wheat stored in the midwestern United States, were calculated and plotted, along with hours of accumulation below 18.3 °C (65°F). Five geographic zones were delineated based on weather data from 53 stations; and a bin-cooling model, which was integrated with a model for …

Automated Nondestructive Detection Of Lnternal Lnsect Infestation Of Wheat Kernels By Using Near-Infrared Reflectance Spectroscopy, Floyd E. Dowell, James E. Throne, James E. Baker

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Wheat kernels infested internally with larvae of 3 primary insect pests of grain, the rice weevil, Sitophilus oryzae (L.); the lesser grain borer, Rhyzopertha dominica (F.); and the Angoumois grain moth, Sitotroga cerealella (Olivier), were scanned with a near-infrared spectrometer incorporated into a single kernel characterization system to determine differences in absorption due to the presence of larvae. The single kernel characterization system delivers kernels into the spectrometer viewing area at the rate of 1 per 4s. We were able to differentiate uninfested kernels from kernels infested with larvae of all 3 species by using this automated system. Moisture content, …

Relationships Among Grain Sorghum Quality Factors, Roberto A. Buffo, Curtis L. Weller, Anne M. Parkhurst

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Correlations among grain sorghum quality factors (proximate composition, physical properties, and water absorption properties) were evaluated. Samples of 46 commercial hybrids (24 and 22 from crop years 1993 and 1994) were analyzed for starch, protein, crude free fat, test weight, absolute density, 1,000 kernel weight, percent kernel abraded, water absorption index, initial water absorption rate, and moisture saturation point. Test weight, absolute density, and percent kernel abraded were positively correlated among themselves (r > 0.5). Protein was negatively correlated with both test weight and absolute density (r < -0.5), while moisture saturation point showed negative correlations with test weight, absolute density, 1,000 kernel weight, and percent kernel abraded (r < -0.4). Principal component factor analysis through the covariance matrix explained 95% of the total variation of quality factors among hybrids (two factors), and, through the correlation matrix, 85% of the total variation (five factors). Water absorption rate decreased with increasing starch content of grain sorghum kernels as water absorption rate increased and amount of water for saturation decreased with softening of kernels.

Opto-Electronic Sensor System For Rapid Evaluation Of Planter Seed Spacing Uniformity, Michael F. Kocher, Yubin Lan, Changhe Chen, John A. Smith

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An opto-electronic seed spacing evaluation system that measured time intervals between seeds and detected front-to-back location of seed drop events relative to the planter was used to rapidly determine planter seed spacing uniformity in the laboratory. The seed detection sensor for the opto-electronic system consisted of a rectangular photogate with 24 phototransistors receiving light beams from 24 LEDs opposite to them. The system also included circuitry to interface the photogate with a digital I/O board in a personal computer. The opto-electronic system was tested with three planter configurations. During the tests, the photogate was positioned beneath the seed drop tube …

Annual Review Of The World Pheasant Association 1997/1998, World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Reports and Other Materials

Chairman's report, Richard P. Howard

Annual report of Council 1997/98

Chapters and Affiliate reports

Pheasant Specialist Group report, Peter J. Garson

Treasurer's annual report and account, Ian F. Hoggarth

Partridge, Quail and Francolin Specialist Group report, John P. Carroll

Grouse Specialist Group report, Ilse Storch

Megapode Specialist Group report, René W. R. J. Dekker

Cracid Specialist Group report, Daniel M. Brooks and Stuart D. Strahl

WPA-South Asia Regional Office, Rahul Kaul

Ecology and conservation of the Moluccan megapode in Halmahera, Gillian C. Baker, Helen Crudgington, Hujaima Cein, and Halim Mahmud

Sichuan hill-partridge forest conservation project: Report on the 1997 field …

Residential Group Homes For Nebraska’S Troubled Youth: An Attractive Alternative To Institutionalization, Jill E. Thomsen

Nebraska Law Review

This Note examines the viability of residential group homes for Nebraska’s troubled teenagers. Part II describes the positive impact that community-based group homes have upon abused and neglected youth. Part III addresses the obstacles facing the creation of such facilities, focusing on difficulties posed by restrictive covenants and municipal zoning efforts which restrict residential land use to “single-family dwellings.” Finally, Part IV proposes that protections offered by the Federal Housing Amendments Act under its “family status” provision, as well as the expressed public policies of this state, provide supplemental support in the movement toward sheltering Nebraska’s troubled youth.

Toxicity And Toxicokinetics Of Cadmium In Capitella Sp. I: Relative Importance Of Water And Sediment As Routes Of Cadmium Uptake, Henriette Selck, Valery E. Forbes, Thomas L. Forbes

Valery Forbes Publications

The importance of dissolved versus sediment-bound cadmium as uptake routes for the deposit-feeding polychaete Capitella species I and the toxicity and toxicokinetics of cadmium from these exposure routes were investigated. Effects were reported as changes in worm growth rate, egestion rate and allometry. Radioactive cadmium (¹⁰⁹Cd) was used as a tracer to examine the uptake (5 d) and subsequent depuration (6 d) of cadmium. Both effects and kinetics were investigated in systems with and without sediment. Individual Capitella sp. I were exposed to (1) dissolved (i.e. –1). Worms in water-only treatments showed negative growth rates, which decreased linearly …

Cropwatch No. 97-26, Dec. 12, 1997, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field reports.............198

Bt corn resource.............199

Farm-saved soybean seed.............201

Seed lab tests.............201

Roundup Ready.............201

Winter Extension meetings.............202

Crop Watch index.............203

Weather update.............206

Regent approved.............206

Crop Watch

Order Form.............206

Intracellular Lipid-Binding Proteins And Their Genes, David A. Bernlohr, Melanie A. Simpson, Ann Vogel Hertzel, Leonard J. Banaszak

Department of Biochemistry: Faculty Publications

Intracellular lipid-binding proteins are a family of low-molecularweight single-chain polypeptides that form 1:1 complexes with fatty acids, retinoids, or other hydrophobic ligands. These proteins are products of a large multigene family of unlinked loci distributed throughout the genome. Each lipid-binding protein exhibits a distinctive pattern of tissue distribution. Transcriptional control, regulated by a combination of peroxisome proliferator activated receptors and CCAAT/enhancer-binding proteins, allows for a variety of both cell and tissue-specific expression patterns. In some cells, fatty acids increase the expression of the lipid-binding protein genes. Fatty acids, or their metabolites, are activators of the peroxisome proliferator–activated receptor family of …

Beef Quality Assurance–Past, Present, Future, Gary C. Smith, J. D. Tatum, K. E. Belk

Range Beef Cow Symposium

The Beef Quality Assurance Task Force (BQATF) was formed in early 1986 when three NCBA (then, the National Cattlemen's Association) Policy Committees independently directed NCBA to address "the growing issue of consumer concern about the safety and wholesomeness of beef." It was believed that the cattle industry's efforts aimed at improving beefs image as a healthful food with regard to its nutrient profile could not stand alone, and that lingering consumer concerns about drug and chemical residues in beef could negate any progress made in the diet/health area. Consequently, the BQATF (then, the Beef Safety Assurance Task Force) was formed …

Front Matter And Contents

Symposium on Double-Crested Cormorants: Population Status and Management Issues in the Midwest

Symposium on Double-Crested Cormorants: Population Status and Management Issues in the Midwest

Diet Of The Double-Crested Cormorant In Western Lake Erie, Michael T. Bur, Sandra L. Tinnirello, Charles D. Lovell, Jeff T. Tyson

Symposium on Double-Crested Cormorants: Population Status and Management Issues in the Midwest

Sport and commercial fishing interest groups are concerned about potential impacts double-crested cormorants (Phalacrocorax auritus) may have on fish species. Our objectives for this study were to determine the diet of the cormorant in western Lake Erie and the diet overlap and competition for resources with piscivorous fish, such as walleye (Stizostedion vitreum.) The stomach contents of 302 double-crested cormorants collected in western Lake Erie consisted primarily of young-of-the-year gizzard shad (Dorosoma cepedianum), emerald shiner (Notropis atherinoides,) and freshwater drum (Aplodinotus grunniens). In the spring, freshwater drum were the most …

Colonial Waterbird Nesting On West Sister Island National Wildlife Refuge And The Arrival Of Double-Crested Cormorants, Mark C. Shieldcastle, Larry Martin

Symposium on Double-Crested Cormorants: Population Status and Management Issues in the Midwest

Recent survey data have shown the importance of West Sister Island National Wildlife Refuge, Lake Erie, to nesting waders. About 40 percent of all herons and egrets nesting in the U.S. Great Lakes are found there, including the Great Lakes’ largest colonies of great blue heron (Ardea herodias), great egret (Ardea alba), and black-crowned nightheron (Nycticorax nycticorax), and the largest of two snowy egret (Egretta thula). West Sister Island’s importance to Ohio has grown in recent decades with the loss of smaller mainland colonies of waders, especially the black-crowned night-heron. The double-crested …

Double-Crested Cormorant Culling In The St. Lawrence River Estuary: Results Of A 5-Year Program, J. Bedard, A. Nadeau, M. Lepage

Symposium on Double-Crested Cormorants: Population Status and Management Issues in the Midwest

Modeling indicated that lowering the doublecrested cormorant population from 17,361 to 10,000 pairs could be attained only by a combination of techniques: culling breeding birds in arboreal colonies to lower breeding stock and egg spraying in accessible ground nests to lower recruitment. The 5-year program was launched in 1989; culling was halted 4 years later because the population had fallen below the threshold of 10,000 breeding pairs. A greater vulnerability of males to shooting (203:100) probably accounted for the faster-than-predicted drop in numbers. Egg spraying spanned the entire 5-year period, during which 25,095 nests were treated with inert mineral oil. …