Digital Commons Network^™

Interactions Between The Structural Domains Of The Rna Replication Proteins Of Plant-Infecting Rna Viruses, Erin K. O'Reilly, Zhaohui Wang, Roy C. French, C. Cheng Kao

Department of Plant Pathology: Faculty Publications

Brome mosaic virus (BMV), a positive-strand RNA virus, encodes two replication proteins: the 2a protein, which contains polymerase-like sequences, and the 1a protein, with N-terminal putative capping and C-terminal helicase-like sequences. These two proteins are part of a multisubunit complex which is necessary for viral RNA replication. We have previously shown that the yeast two-hybrid assay consistently duplicated results obtained from in vivo RNA replication assays and biochemical assays of protein-protein interaction, thus permitting the identification of additional interacting domains. We now map an interaction found to take place between two 1a proteins. Using previously characterized 1a mutants, a perfect …

A Sensitive Elisa For Pythium Ultimum Using Polyclonal And Species-Specific Monoclonal Antibodies, G. Y. Yuen, J. Q. Xia, C. L. Sutula

Department of Plant Pathology: Faculty Publications

A double-antibody sandwich indirect enzyme-linked immunosorbent assay (ELISA) was developed for the detection and quantification of Pythium ultimum. A polyclonal antibody produced to cell walls of P. ultimum was used as the capture antibody, while a P. ultimum-specific monoclonal antibody (MAb E5) was used for recognition of the fungus. In the ELISA, culture extracts of 7 isolates of P. ultimum exhibited strong positive reactions, whereas none of the 37 isolates of other Pythium spp. and fungal genera had positive reactions. P. ultimum was detected by ELISA in roots of bean, cabbage, and sugar beet seedlings grown in pathogen-infested …

Mutational Analysis Of Area, A Transcriptional Activator Mediating Nitrogen Metabolite Repression In Aspergillus Nidulans And A Member Of The “Streetwise” Gata Family Of Transcription Factors, Richard A. Wilson, Herbert N. Arst Jr.

Fungal Molecular Plant-Microbe Interactions

Details a mutational analysis of AREA, a transcriptional activator mediating nitrogen metabolite repression in Aspergillus nidulans and a member of the “streetwise” GATA family of transcription factors.

The Plant Cdna Lct1 Mediates The Uptake Of Calcium And Cadmium In Yeast, Stephen Clemens, Danuta M. Antosiewicz, John M. Ward, Daniel P. Schachtman, Julian I. Schroeder

Department of Agronomy and Horticulture: Faculty Publications

Nonessential metal ions such as cadmium are most likely transported across plant membranes via transporters for essential cations. To identify possible pathways for Cd²⁺ transport we tested putative plant cation transporters for Cd²⁺ uptake activity by expressing cDNAs in Saccharomyces cerevisiae and found that expression of one clone, LCT1, renders the growth of yeast more sensitive to cadmium. Ion flux assays showed that Cd²⁺ sensitivity is correlated with an increase in Cd²⁺ uptake. LCT1-dependent Cd²⁺ uptake is saturable, lies in the high-affinity range (apparent K_M for Cd²⁺ = 33 µM) and is …

Biodegradation Of Atrazine By Agrobacterium Radiobacter J14a And Use Of This Strain In Bioremediation Of Contaminated Soil, J. K. Struthers, K. Jayachandran, T. B. Moorman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We examined the ability of a soil bacterium, Agrobacterium radiobacter J14a, to degrade the herbicide atrazine under a variety of cultural conditions, and we used this bacterium to increase the biodegradation of atrazine in soils from agricultural chemical distribution sites. J14a cells grown in nitrogen-free medium with citrate and sucrose as carbon sources mineralized 94% of 50 μg of [¹⁴C-U-ring]atrazine ml^-1 in 72 h with a concurrent increase in the population size from 7.9 x 10⁵ to 5.0 x 10⁷ cells ml^-1. Under these conditions cells mineralized the [ethyl-¹⁴C]atrazine …

Alternative Splicing Of The Latency-Related Transcript Of Bovine Herpesvirus 1 Yields Rnas Containing Unique Open Reading Frames, Laxminarayana R. Devireddy, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

The latency-related transcript (LRT) of bovine herpesvirus 1 (BHV-1) is the only abundant viral RNA detected during latency. A previous study (A. Hossain, L. M. Schang, and C. Jones, J. Virol. 69:5345–5352, 1995) concluded that splicing of polyadenylated [poly(A) ⁺] and splicing of nonpolyadenylated [poly(A) ^-] LRT are different. In this study, splice junction sites of LRT were identified. In trigeminal ganglia of acutely infected calves (1, 7, or 15 days postinfection [p.i.]) or in latently infected calves (60 days p.i.), alternative splicing of poly(A) ⁺ LRT occurred. Productive viral gene expression in trigeminal ganglia is readily detected …

Radial Distribution Of Electron Spectra From High-Energy Ions, Francis A. Cucinotta, Robert Katz, John W. Wilson

Robert Katz Publications

The average track model describes the response of physical and biological systems using radial dose distribution as the key physical descriptor. We report on an extension of this model to describe the average distribution of electron spectra as a function of radial distance from an ion. We present calculations of these spectra for ions of identical linear energy transfer (LET), but dissimilar charge and velocity to evaluate the differences in electron spectra from these ions. To illustrate the usefulness of the radial electron spectra for describing effects that are not described by electron dose, we consider the evaluation of the …

Cloning And Expression Of The Listeria Monocytogenes Scott A Ptsh And Ptsi Genes, Coding For Hpr And Enzyme I, Respectively, Of The Phosphotransferase System, Douglas P. Christensen, Andrew K. Benson, Robert W. Hutkins

Department of Food Science and Technology: Faculty Publications

The phosphoenolpyruvate (PEP)-dependent phosphotransferase system (PTS) utilizes high-energy phosphate present in PEP to drive the uptake of several different carbohydrates in bacteria. In order to examine the role of the PTS in the physiology of Listeria monocytogenes, we identified the ptsH and ptsI genes encoding the HPr and enzyme I proteins, respectively, of the PTS. Nucleotide sequence analysis indicated that the predicted proteins are nearly 70% similar to HPr and enzyme I proteins from other organisms. Purified L. monocytogenes HPr overexpressed in Escherichia coli was also capable of complementing an HPr defect in heterologous extracts of Staphylococcus aureus ptsH …

Residual Effects Of Crop Residues On Grain Production And Selected Soil Properties, James F. Power, Paul Koerner, John W. Doran, Wallace Wilhelm

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Returning crop residue improves water conservation and storage, nutrient availability, and crop yields. We have little knowledge, however, of the residual impacts of crop residues on soil and crop production. We hypothesized that residual impacts of crop residues vary with the amount of residues used. A 10-yr study near Lincoln, NE, evaluated the residual effects of an earlier 8-yr study of various crop residue amounts on crop growth and selected soil properties. From 1978 through 1985, crop residues were returned at 0,50, 100, and 150% of the quantity produced by the previous crop (averaging 0 to =6 Mg ha^{-1 …}

Bats Of The Antillean Island Of Grenada: A New Zoogeographic Perspective, Hugh H. Genoways, Carleton J. Phillips, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

The island of Grenada is the southernmost of the Lesser Antilles, lying 130 km north of Trinidad and 135 km north of the Venezuelan mainland. It measures 34 km north to south and 19 km east to west and has an area of 312 square km. Grenada and the Grenadines northward to Bequia stand on the large submarine Grenada Bank. At 183 m depth, the bank is 179 km long. The Grenadines cover the bank to its northern end, but the bank extends for 39 km south of Grenada with no islands. During the last Ice Age, Grenada and the …

Strategies For Sustainable Economic Development, Duane Olsen

Cornhusker Economics

During a conference highlighting "sustainable Communities," Margaret Thomas presented a paper based upon a 1994 research project designed to explore economic development strategies that would increase local prosperity and also protect the environment and protect natural resources. She described the economy and ecology as two sides of the same coin and proposed reconsidering human systems and industrial systems in the light of these two natural laws: 1) resources are to be used no faster than they are replenished, and 2) matter is ultimately recycled for reuse in biological or physical systems.

Cropwatch No. 98-20, Aug. 14, 1998, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Wheat videoconference........176

Predicting last irrigation........177

Map weed problems........180

Dry Bean Field Day........181

Herbicide Use Guide........181

Field updates........181

Planting alfalfa........182

GDD........182

Crop water use........182

Starling Management In Agriculture, Ron J. Johnson, James F. Glahn

Other Publications in Wildlife Management

European starlings (Sturnus vulgaris) were brought to the United States from Europe. They were released in New York City in 1890 and 1891 and, since that time, have spread across the country. They were observed in the midwest by 1930, in Colorado in 1939, and in California in 1942. The starling population in the United States has since grown to an estimated 140 million birds. Starlings are frequently considered pests because of the damage they cause, especially to agriculture and in urban roosts. This publication provides facts about starlings and methods to control their damage in agriculture.

Plans For Crash-Tested Bridge Railings For Longitudinal Wood Decks On Low-Volume Roads, Michael A. Ritter, Ronald K. Faller, David B. Bunnell, Paula D. Hilbrich Lee, Barry Thomas Rosson

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The plans for crashworthy bridge railings for low-volume roads were developed through a cooperative research program involving the USDA Forest Service, Forest Products Laboratory (FPL); the Midwest Roadside Safety Facility, University of Nebraska-Lincoln (MwRSF); and the Forest Service, National Forest System, Engineering. Three railings were developed and successfully tested in accordance with National Cooperative Highway Research Program (NCHRP) Report 350 Test Level-1 requirements. The fourth system was developed for a lower test level based on criteria developed by the Forest Service for single-lane bridges on very low-volume roads. For the convenience of the user, full drawing sets are provided in …

Nebline, August 1998

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Harvesting Everlasting Flowers for Drying
Watering tips
Scorch—curse of plants on the plains
New grass faces the big test
Storing extra seeds
Control of ground ivy in the lawn
Audubon purchases 610-acre O'Brien Ranch
Household hazardous waste collection
Don't get stung!
Allergic to bee stings?
Dealing with unwanted guests
How animals stay cool when it's hot!
Fruit, vegetables and freedom to farm
Prescribed burning of CRP
What is LB1209 and does it affect you?
Toxic plants
How often to pump your septic tank
Caring for animals when gone
Flies
Field Day features high-value crops
Buildings
Festival of Color
Fresh, fabulous …

Ard News August 1998

Agricultural Research Division: News and Annual Reports

CONTENTS:

Comments from the Dean
Additional Opportunities for the ARD Undergraduate Honors Student Research Program
Widaman Trust Distinguished Graduate Assistant Award
Hardin Distinguished Graduate Fellowship for 1998-1999
ARD Advisory Council Election Results
Proposals Submitted for Federal Grants
Grants and Contracts Received June and July, 1998
New or Revised Projects
Nancy Lewis Selected for Leadership Development Program
Additional Guidance Regarding Tuition Remission on USDA-Funded Graduate Research Assistantships
Cooperative State Research, Education and Extension Service Budget for FY 1999
NUPROF Program
Fund for Rural America Update

Starling Management In Agriculture, Ron J. Johnson, James F. Glahn

Other Publications in Wildlife Management

European starlings (Sturnus vulgaris) were brought to the United States from Europe. They were released in New York City in 1890 and 1891 and, since that time, have spread across the country. They were observed in the midwest by 1930, in Colorado in 1939, and in California in 1942. The starling population in the United States has since grown to an estimated 140 million birds. Starlings are frequently considered pests because of the damage they cause, especially to agriculture and in urban roosts. This publication provides facts about starlings and methods to control their damage in agriculture.

The Probe, Issue 191 - August 1998

The Probe: Newsletter of the National Animal Damage Control Association

You Can't Win if You Don't Play -- Diane deLorimier, NADCA Regional Director, Western Region (Region 1), Sutton Ag Enterprises, Salinas, California
The 2nd Edition of the Field Manual of Wildlife Diseases in the Southeastern United States is now available for purchase.
Canadians Test Bone Oil as Coyote Repellent
Trap Injury Study Begins
Video review: "Intermediate Coyote Trapping" by Tom Beaudette of High Country Control. 1998.1-1/2 hours. VHS. $38.00
Arson Damages USDA Olympia Facilities
Important Ruling in Alaska Wolf Snare Dispute
Utah Man Charged for Poisoning Gulls
Girls Scouts Licensed to Kill Geese
Texas Animal Damage Control Service has changed …

A Partial Host Range Of The High Plains Virus Of Corn And Wheat, Dallas L. Seifers, Tom L. Harvey, T. J. Martin, Stanley G. Jensen

Department of Plant Pathology: Faculty Publications

Barley (Hordeum vulgare), cheat (Bromus secalinus), corn (Zea mays), oat (Avena sativa), rye (Secale cereale), and wheat (Triticum aestivum) were infected by a Kansas isolate of the High Plains virus (HPV) in greenhouse experiments, but several other grass species were not. Infection of a host was dependent upon wheat curl mite numbers. Although both green foxtail (Setaria viridis) and yellow foxtail (S. glauca) are found naturally infected by HPV, only yellow foxtail could be infected in greenhouse experiments. Field sampling (1994 to 1996) of …

Water Current, Volume 30, No. 4, August 1998

Water Current Newsletter

Tent Talks, Demonstrations Highlight Sixth Annual Festival of Color, September 12
NebFacts Suggest Ways to Reduce Blue River Basins Runoff Pollution
Wetlands Tabloid Available
AWWA Research Proposals
Four Cities Hosting Third Wetlands Month Conference
UNL Seminar Series VCR Tapes Available

Plans For Crash-Tested Wood Bridge Railings For Concrete Decks, Michael A. Ritter, Ronald K. Faller, Paula D. Hilbrich Lee, Barry Thomas Rosson, Sheila Rimal Duwadi

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

As part of a continuing cooperative research between the Midwest Roadside Safety Facility (MwRSF); the USDA Forest Service, Forest Products Laboratory (FPL); and the Federal Highway Administration (FHWA), several crashworthy wood bridge railings and approach railing transitions have been adapted for use on concrete bridge decks. These railings meet testing and evaluation criteria outlined in National Cooperative Research Program (NCHRP) Report 350, Recommended Procedures for the Safety Performance Evaluation of Highway Features, and include a glued-laminated timber (glulam) rail, with and without a curb, at Test Level- 2 (TL-2), a glulam rail with curb at TL-4, and a glulam …

Cropwatch No. 98-16, July 9,1998, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field updates………………..138

Pesticide labels on-line………………..144

Insect update………………..145

Corn root injury………………..146

Irrigating soybeans………………..147

Weeds in wheat………………..149

Wipers and bean bars………………..150

Precipitation………………..150

Irrigating alfalfa………………..151

GDD and ETs………………..152

Phylogenetic Relationships Within The Family Potyviridae: Wheat Streak Mosaic Virus And Brome Streak Mosaic Virus Are Not Members Of The Genus Rymovirus, Drake C. Stenger, Jeffrey S. Hall, Il-Ryong Choi, Roy C. French

Department of Plant Pathology: Faculty Publications

The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5&#;&#;leader and 149-nt 3&#;-untranslated region and is polyadenylated at the 3&#;&#;end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5&#;-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3&#;). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is …

Covalently Functionalized Nanotubes As Nanometer-Sized Probes In Chemistry And Biology, Stanislaus S. Wong, Ernesto Joselevich, Adam T. Woolley, Chin Li Cheung, Charles M. Lieber

Barry Chin Li Cheung Publications

Carbon nanotubes combine a range of properties that make them well suited for use as probe tips in applications such as atomic force microscopy (AFM)^{1, 2, 3}. Their high aspect ratio, for example, opens up the possibility of probing the deep crevices⁴ that occur in microelectronic circuits, and the small effective radius of nanotube tips significantly improves the lateral resolution beyond what can be achieved using commercial silicon tips⁵. Another characteristic feature of nanotubes is their ability to buckle elastically^{4, 6}, which makes them very robust while limiting the maximum force that is …

Recovery Plan For The Blue Whale (Balaenoptera Musculus), Randall R. Reeves, Phillip J. Clapham, Robert L. Brownell Jr., Gregory K. Silber

United States Department of Commerce: Staff Publications

Recovery plans identify reasonable actions which are believed to be required to recover and/or protect endangered species. Plans are prepared by the National Marine Fisheries Service (NMFS) and the U.S. Fish and Wildlife Service (FWS) and sometimes with the assistance of recovery teams, contractors, State agencies, and others. This plan was prepared by Randall R. Reeves, Phillip J. Clapham, Robert L. Brownell, Jr., and Gregory K. Silber for NMFS. Recovery plans do not necessarily represent the views nor the official positions or approvals of any individuals or agencies, other than those of NMFS, and they represent the views of NMFS …

Seasonal Variation In Reception Of Fin Whale Calls At Five Geographic Areas In The North Pacific

United States Department of Commerce: Staff Publications

In late August 1991 scientists at the National Oceanic and Atmospheric Administration’s (NOAA) National Marine Mammal Laboratory (NMML) and Pacific Marine Environmental Laboratory (PMEL) began a pilot study to investigate the capability of hydrophones from the US. Navy’s fixed array system to detect large whales in the North Pacific by passive reception of their calls. PMEL had previously established a direct data link from five bottom-mounted arrays of the Navy SOSUS (Sound Surveillance System), via the Naval Oceanographic Processing Facility (NOPF) at Whidbey Island, Washington, to study low-level seafloor seismicity (Fox et al. 1994). PMEL subsequently provided NMML tapes of …

Center For Grassland Studies Newsletter, Summer 1998, Volume 4, No. 3

Center for Grassland Studies: Newsletters

Contents:

Nebraska's Wetlands by Ted LaGrange,Nebraska Game and Parks Commission

Ashfall Fossil Beds: Record of an Ancient Grassland Ecosystem Preserved in Rock by Michael Voorhies, University of Nebraska State Museum

Wet Meadow Restoration at The Nature Conservancy's Speidell Tract by Chris Helzer, The Nature Conservancy

CGS Advisory Committee Tours Golf Course, Burned Grasslands, Fossil Beds and Ranch

Canadian Cooperative Wildlife Health Centre, Volume 5, Summer 1998

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

CCWHC Involvement with Botulism
The Value of A Complete Necropsy in Controversial Situations
Yersiniosis in Snowshoe Hares - Newfoundland
Mass mortality of young harp seals
Salmonellosis in passerine birds
Canine Parvovirus Type 2 Infection in a Wild Wolf
Presumptive Atoxoplasmosis in Common Grackles
Evidence of Leptospira infection in raccoons from Middlesex and Kent counties, Ontario
Toxicity Events

Review Of The Culture Of Wilderness: Agriculture As Colonization In The American West By Frieda Knobloch, Emily Greenwald

Great Plains Quarterly (through 2013)

In this intriguing synthesis, Frieda Knobloch brings a new set of ideas to existing scholarship on agriculture and the environmental history of the American West. She traces the process of "nature becoming culture," the ways in which naturally existing resources (land, animals, plants, and trees) were transformed into agriculturally-managed commodities, thereby serving the twin imperatives of colonization and agriculture. Knobloch argues that colonization and agriculture not only sprang from the same roots in the English language, but have also operated hand-in-hand to dispossess indigenous plants, animals, and peoples. In Knobloch's formulation, neither can exist apart from the other-essentially, agriculture is …

Canadian Cooperative Wildlife Health Centre, Volume 5-2, Summer 1998

Canadian Cooperative Wildlife Health Centre: Newsletters and Publications

CCWHC Involvement with Botulism
The Value of a Complete Necropsy in Controversial Situations
Yersiniosis in Snowshoe Hares - Newfoundland
Mass mortality of young harp seals
Salmonellosis in passerine birds
Canine Parvovirus Type 2 Infection in a Wild Wolf
Presumptive Atoxoplasmosis in Common Grackles
Evidence of Leptospira infection in raccoons from Middlesex and Kent counties, Ontario
Toxicity Events
Canine Distemper Virus in Skunks
Assessment of Risk related to Translocation of Elk into Saskatchewan
Vancouver Island Marmots
Anomalous Black bear
Osprey vs. Muskrat (A Fatal Tie?)
Canine Distemper in Coyotes and a Wolf in Alberta