Digital Commons Network^™

Posttranscriptional Regulation Of Mammalian Methionine Synthase By B12, Sumedha Gulati, Lawrence C. Brody, Ruma V. Banerjee

Department of Biochemistry: Faculty Publications

Methionine synthase is one of two key enzymes involved in the removal of the metabolite, homocysteine. Elevated homocysteine levels constitute a risk factor for cardiovascular diseases and for neural tube defects. In cell culture, the activity of methionine synthase is enhanced several-fold by supplementation with its cofactor, B₁₂. The mechanism of this regulation is unknown, although it has been ascribed to a shift from apoenzyme to holoenzyme. Using sensitive assay techniques as well as a combination of Northern and Western analyses, we demonstrate that the effect of B,, on induction of methionine synthase activity is paralleled by an …

Hexapod Herald - Vol. 11, No. 3, July 7, 1999

Hexapod Herald and Other Entomology Department Newsletters

Calendar of events

Grants

Open letter from Sharron Quisenberry

Congratulations

Welcome

Faculty news

Graduate student news

Publications

Travel

From the office

Cropwatch No. 99-16, July 2,1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field updates………….142

Rootworm, corn and wind………….143

Diagnostic update………….143

Weather trivia………….144

First irrigation………….145

Accessing TE, GDD data………….145

Balance update………….146

Weed control in pastures………….147

Crop and precip updates………….148

Fish & Wildlife News: July/August 1999

Fish and Wildlife News

Contents:

Bald Eagle Soaring to Recovery 2
New Directorate Appointments 3
Biologists Discover Lynx Kittens 7
Preview National Wildlife Refuge Week 9
Refuge Management Goes Under the Sea 13
Learning About Fisheries in Region 1 14
Student Career Program Fulfills a Promise 18
Service Reaches Out to Ranchers 21
FWS and Tribes: Partnership Success Story 21
Tracking the Elusive Sage Grouse 24
New Feature: Fish & Wildlife Honors... 30
Fish & Wildlife...In Brief 31

Center For Grassland Studies Newsletter, Summer 1999, Volume 5, No. 3

Center for Grassland Studies: Newsletters

Contents:

Managing Intensively Used Turfs by Robert (Bob) Shearman, Department of Horticulture, UNL

Management Practices and Plant Diversity on Some Privately-Owned Meadows in the Middle Loup River Valley by Steven Rothenberger, Department of Biology, University of Nebraska at Kearney

Grassland Ecology Is Theme of CGS Seminar Series

Grazing Lands Technical Assistance Available

Rancher's Forum October 6

Regional Grazing Project Approved

Moderate Grazing Promotes Plant Diversity

Looking for a Few Good Forage Publications

American Society Of Parasitologists Newsletter, V. 21, No. 2, [Summer 1999], Scott Lyell Gardner

American Society of Parasitologists: Newsletter

An issue of the American Society of Parasitologists' quarterly newsletter, also called the Journal of Parasitology Newsletter.

The Probe, Issue 202 – July 1999

The Probe: Newsletter of the National Animal Damage Control Association

Bird Strike USA Meeting a Success -- Eugene LeBoeuf, Bird Strike Scientist, HQ Air Force Safety Center, KirtlandAFB, New Mexico, and Past President, NADCA
Position Available: Wildlife Biologist, JFK Airport
Hawk Starts Grass Fire
Juicy Fruit Gum Remedy Story Persists
Cambodian Artifacts Threatened by Bats
Predator Killings Planned: State Game Managers Target Cougars, Coyotes
Video review: "Cleaning Skulls for Pleasure or Profit" by Ken Carver, Maplewood, MN. VHS. Approx. 120 minutes. $19.95 postpaid.
House Backs Funding of Program That Targets Predators
Armadillos Considered Delicacies By Some; Leprosy Threat by Others

Mabel Lee And Louise Pound: The University Of Nebraska's Battle Over Women's Intercollegiate Athletics, Kristi Lowenthal

Department of History: Dissertations, Theses, and Student Research

Mirroring a cultural shift that brought the American middle class from the Victorian era into the modern era, women's intercollegiate sports encountered stiff resistance from the two overlapping social groups. Mabel Lee, a woman physical educator, brought firmly held Victorian beliefs about feminine propriety and moderation to the University of Nebraska when she was hired as the head of the women's physical education department in 1924. Louise Pound, an English professor at the University, had for years excelled in highly competitive athletics as a member of the new modern middle class. Pound flouted Victorian restraints and refused to enter the …

Journal Of Telecommunications In Higher Education

ACUTA Journal

In This Issue

6 Meeting Bandwidth Challenges on Campus

12 Voice-over-LAN: A Solution for Convergence in the Enterprise

20 Going Beyond Best Effort IP Networking

25 The Politics of Convergence

32 Pursuing the Promise of the Paperless Office

38 New Visions for University Cellular Service

44 Maintaining Excellence at UMB

Center For Sustainable Agricultural Systems Newsletter, July/August 1999

Center for Sustainable Agricultural Systems: Newsletter (1993–2000)

Contents:

Biotechnology Issues Discussed at NABC Meeting in Lincoln

University Role in Biotechnology: How Do We Sustain Food Production?

CSAS Issues Volumes 10 and 11

SARE Marketing Conference in Lincoln This November

September 10 is Deadline for NCR SARE Preproposals

Nebraska Legislative Bills Study Management and Help Beginning Farmers/Ranchers

Voluntary Program Will Boost Organic Exports

Canada Introduces National Standard for Organic Agriculture

Merrigan to Head USDA's Agricultural Marketing Service

Nominations Sought for Steward of the Land Award

Thanks and Farewell, Glen

Scwds Briefs: Volume 15, Number 2 (July 1999)

Southeastern Cooperative Wildlife Disease Study: Publications

SCWDS BRIEFS
July 1999
1998 HD Summary
Hemorrhagic disease (HD)
EHD virus serotype 2 (EHDV-2)
Viral RNA
HD Virus Isolation Support
Epizootic hemorrhagic disease virus (EHDV)
Bluetongue Virus (BTV)
Cattle pulmonary artery endothelial (CPAE)
BHK21 cells
Recommendations for the Use of Lyme Disease Vaccine
Lyme disease
Drug Enforcement Administration (DEA)
Ketamine
Avian Brain Lesion Syndrome (ABLS)
Coot and Eagle Brain Lesion Syndrome (CEBLS)
Harold M. Smith -- 1942-1999

Nebline, July 1999

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

A day at the fair: Is competition good or bad?
Vegetable gardening in the fall
1999 “Plants of the Year” recommended for Nebraska
Water use in the landscape
Watch for Squash Vine Borer
Powdery mildew on ornamentals
Are you afraid of snakes? spiders? insects?
Master beekeeping and queen rearing workshops
Tread carefully when hiking in Nebraska: watch for poisonous snakes
Fertilizing grass pastures and hay lands-Part 2
Scouting for rootworm beetles could save big money next year
Be alert to the danger; poison hemlock abounds this year
Tips for late summer alfalfa planting
Field day will feature profitable crops and …

Challenges For Directors Of University Natural Science Museums, Hugh H. Genoways

University of Nebraska State Museum: Mammalogy Papers

Universities and natural science museums have a long, productive history; however, this has been an uneasy alliance in the United States at least since the 1880s. Decreasing resources and increasing expectations have made the position of all museum directors extremely difficult, but the situation for university natural science museum directors is probably the most complicated among these because they direct museums that are small administrative units within larger university organizations. Some of their challenges include conflict between museum and university missions, governance issues, relationship between director and the university administrator/board member, lack of understanding of museum functions, middle management role …

Dnak And The Heat Stress Response Of Pseudomonas Syringae Pv. Glycinea, Lisa M. W. Keith, James E. Partridge, Carol L. Bender

Department of Plant Pathology: Faculty Publications

The dnaK gene from Pseudomonas syringae pv. glycinea PG4180 was cloned and sequenced. The dnaK coding region was 1,917 bp and contained a putative O³² heat shock promoter 86 bp upstream of the translational start site. grpE, another heat shock gene, was found immediately upstream of the putative dnaK promoter. The predicted amino acid sequence of dnaK showed relatedness to the ATPase and substrate binding domains commonly found in heat shock proteins, as well as the highly conserved signature sequence motifs belonging to the Hsp70 protein family. Furthermore, the PG4180 dnaK gene complemented an Escherichia coli dnaK mutant …

Morphogenesis Is Coordinated With Nuclear Division In Germinating Aspergillus Nidulans Conidiospores, Steven D. Harris

Department of Plant Pathology: Faculty Publications

Germinating Aspergillus nidulans conidiospores switch to polarized apical growth following an initial period of isotropic expansion. At the same time, they re-enter the nuclear division cycle. The relationship between spore polarization and nuclear division was investigated by testing the effect of cell cycle inhibitors and temperature-sensitive cell cycle mutations on spore morphogenesis. On rich media, it was found that spore polarization is delayed if completion of the first mitosis is blocked. The observed delay may be dependent upon the activity of the mitosis-promoting NIMA kinase. An additional mechanism appears to prevent polarization as the spore progresses through its first S …

Optimal Replication Activity Of Vesicular Stomatitis Virus Rna Polymerase Requires Phosphorylation Of A Residue(S) At Carboxy-Terminal Domain Ii Of Its Accessory Subunit, Phosphoprotein P, Leroy N. Hwang, Nathan Englund, Tapas Das, Amiya K. Florida, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein, P, of vesicular stomatitis virus (VSV) is a key subunit of the viral RNA-dependent RNA polymerase complex. The protein is phosphorylated at multiple sites in two different domains. We recently showed that specific serine and threonine residues within the amino-terminal acidic domain I of P protein must be phosphorylated for in vivo transcription activity, but not for replication activity, of the polymerase complex. To examine the role of phosphorylation of the carboxy-terminal domain II residues of the P protein in transcription and replication, we have used a panel of mutant P proteins in which the phosphate acceptor sites …

Phosphorus And Nitrogen In Runoff Following Beef Cattle Manure Of Compost Application, Bahman Eghball, John E. Gilley

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Manure or compost from beef cattle feedlots can be an excellent sources of nutrients and organic matter when added to soils, but they can also pollute runoff. We determined the effects of simulated rainfall on runoff losses of P and N, and EC and pH following application of manure and compost to a Sharpsburg silty clay loam (fine smectitic, mesic Typic Argiudoll) soil having grain sorghum [Sorghum bicolor (L.) Moench] and winter wheat (Triticum aestivum L.) residues. Manure, compost, and fertilizer were applied to no-till fields at rates required to meet N or P requirements for corn ( …

Density-Dependent Effects Of A Toxicant On Life-History Traits And Population Dynamics Of A Capitellid Polychaete, Inez Linke-Gamenick, Valery E. Forbes, Richard M. Sibly

Valery Forbes Publications

The toxic effects of the widespread polycyclic aromatic hydrocarbon (PAH), fluoranthene (FLU), on Life-history traits of Capitella sp. M as a function of population density were investigated in a life-table-response-experiment (LTRE) lasting 134 d. Animals from laboratory cultures were exposed to 6 different FLU concentrations (range 0 to 80 μg FLU [g dry wt sed]^–1, ppm) at 3 population densities (range 529 to 36 842 worms m^–2), and survivorship, growth rate until maturity, and reproductive parameters were recorded. Fluoranthene exposure significantly reduced juvenile survivorship in a concentration- dependent manner, whereas population density had no effect on …

Agricultural Research Division 113th Annual Report 1999

Agricultural Research Division: News and Annual Reports

It is a pleasure to provide you with a copy of the 113th Annual Report of the University of Nebraska Agricultural Research Division (ARD). This report is our opportunity to inform you about our research programs and accomplishments and to allow us to assess the progress and effectiveness of our collective efforts to develop new knowledge. After examining this report, we hope you will agree with our assessment that ARD research has provided new technology and knowledge for Nebraskans which will improve the profitability of their enterprises, enhance environmental quality, and improve their quality of life.

This report provides some …

Cropwatch No. 99-15, June 25, 1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Post control in sorghum………………132

Field updates………………134

Wheat diseases………………135

Sand chafers………………135

Imported weevils………………136

Corn diseases developing………………137

Purple loosetrife………………138

Stewart’s wilt………………139

Effects of weather………………140

Intermediate Species Possessing Bent Dna Are Present Along The Pathway To Formation Of A Final Tbp-Tata Complex, Kay M. Parkhurst, Robyn M. Richards, Michael Brenowitz, Lawrence J. Parkhurst

Lawrence Parkhurst Publications

Binding of the TATA-binding protein (TBP) to the “TATA” sequences present in the promoters of eukaryotic class II genes is the first step in the sequential assembly of transcription pre-initiation complexes. Myriad structural changes, including severe bending of the DNA, accompany TBP-TATA complex formation. A detailed kinetic study has been conducted to elucidate the mechanistic details of TBP binding and DNA bending. The binding of Saccharomyces cerevisiae TBP to the adenovirus major late promoter (AdMLP) was followed in real-time through a range of temperatures and TBP concentrations using fluorescence resonance energy transfer (FRET) and stopped-flow mixing. The results of association …

Methylene-Only Subspectra In 13C Cpmas Using A New Double Quantum Filtering Sequence, Paola Rossi, Raju Subramanian, Gerard S. Harbison

Gerard S. Harbison Publications

Methodology for the assignment of ¹³C CPMAS spectra is still in its infancy. Previous methods of CPMAS spectral editing have utilized differences in the strength of the ¹³C–¹H dipolar interaction or the rate and spin thermodynamics of crosspolarization from protons to carbon, to differentiate between quaternary, tertiary, and methylene carbons. We introduce a different approach, which is based on the fact that double-quantum coherence develops between the protons of a methylene group considerably faster than between most other proton spin pairs in an organic solid. We generate this coherence, filter it, convert it back to single …

Cropwatch No. 99-12, June 4,1999, Lisa Brown Jasa

Crop Watch (1994–2005)

Inside

Field updates ………………..106

GMOs and special traits………………..107

Input/output traits………………..108

GMO issues………………..109

Replant options………………..111

Corn rootworms………………..112

European corn borer worksheet………………..114

Stand Structure And Productivity Of The Rhizophora Mangle In Hawaii, Evelyn F. Cox, James Allen

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Since its introduction in the early part of this century, Rhizophora mangle L. has spread extensively through most of the main islands of the Hawaiian Archipelago. We investigated the structural properties and estimated productivity of a R. mangle population at Nuupia Ponds Wildlife Management Area (NPWMA), on windward Oahu, where the mangroves were being controlled due to their propensity to overgrow archaeological sites and the habitat of endangered Hawaiian waterbirds. Mangroves within NPWMA were very dense (> 24,000 trees ha^-1) and most were relatively small (only 3.3% of the trees were ≥ 10 cm DBH). Mean basal area, …

Ard News June 1999

Agricultural Research Division: News and Annual Reports

CONTENTS:
Comments from the Dean
National Research Initiative Awards for FY 1998
Barta Brothers Ranch Development
Land-Grant Universities Stimulate Economic Growth
Undergraduate Honors Research Program
Awards From Special Endowments
New or Revised Projects
The Future of Science and Technology
Grants and Contracts Received April and May, 1999
Proposals Submitted for Federal Grants
1998 Graduate Student Survey

The Probe, Issue 201 – June 1999

The Probe: Newsletter of the National Animal Damage Control Association

Reflections On NADCA's Two Decades -- Robert M. Timm, PROBE Editor
Video review: "Whitetails at the Crossroads" Northeast Deer Technical Committee, Griff en Productions (produced with a grant from Wildlife Restoration Funds, U.S. Fish and Wildlife Service). 1996. 30 minutes.
Coyote Subdued in Central Park, New York
Supplement: The insert in this issue is a retrospective, gleaned from the 100+ issues of THE PROBE that 'Fitz' composed and published. For some of you long-time NADCA members, this will bring back memories and recollections of the issues of the 80s, as the federal ADC agency (and all ADC practitioners) struggled to …

Nebraska Bird Review (June 1999) 67(2), Whole Issue

Nebraska Bird Review

Spring Field Report, March to May 1999 ... 42

Species Accounts ... 44

Book Review by Linda R. Brown of Swallow Summer ... 71

Neotropic Cormorant at Sutherland Reservoir ... 72

NOU Fall Field Days Count, 1998; May NAMC Count, 1999 ... 73

Clark’s Nutcracker at Lake McConaughy ... 79

Roseate Spoonbill in Otoe County ... 80

Red-throated Loon at Lake McConaughy ... 81

Two Poems by Don Welch and Drawings by Mark Sanders ... 82

Spring Field Report, March To May 1999, W. Ross Silcock, Joel G. Jorgensen

Nebraska Bird Review

This spring proved an exciting season for several reasons. For warbler fans, it described a dream with lots of birds to look at and a rather incredible species count (for anywhere) of no fewer than 381 Many "eastern" warblers were found westward, especially at "islands" of habitat such as the Geneva Cemetery and similar spots just beyond the western edge of the original eastern woodland region, as well as the Panhandle. For rarity counters, spring included no fewer than 4 first state records: Glossy Ibis, Gray Flycatcher, Black-throated Gray Warbler, and Brambling. We make here a special note that the …

The Prairie Naturalist Volume 31, No.2 June 1999

The Prairie Naturalist

SPATlAL VARIATION IN POPULATION CHARACTERISTICS OF SHOVELNOSE STURGEON IN THE KANSAS RIVER ▪ M. C. Quist and C. S. Guy

INFLUENCE OF FIRE AND TRAPPING EFFORT ON GROUND BEETLES IN A RECONSTRUCTED TALLGRASS PRAIRIE ▪ K. J. Larsen and J. B. Williams

FISH ASSEMBLAGES AND HABITAT RELATIONSHIPS IN A SMALL NORTHERN GREAT PLAINS STREAM ▪ C. A. Barfoot and R. G. White

CHRISTMAS BIRD COUNTS FOR NORTH DAKOTA 1998 ▪ R. N. Randall

NEW RECORDS OF THE PYGMY SHREW IN SOUTHEAST SOUTH DAKOTA ▪ C. B. King, G. M. Wilson, and P. D. Sudman

BUFFLEHEAD BROOD IN NORTHEASTERN SOUTH DAKOTA …

Broad-Spectrum Protection Against Tombusviruses Elicited By Defective Interfering Rnas In Transgenic Plants, Teresa Rubio, Marise Borja, Herman Scholthof, Paul A. Feldstein, Thomas Jack Morris, Andrew O. Jackson

Nebraska Center for Virology: Faculty Publications

We have designed a DNA cassette to transcribe defective interfering (DI) RNAs of tomato bushy stunt virus (TBSV) and have investigated their potential to protect transgenic Nicotiana benthamiana plants from tombusvirus infections. To produce RNAs with authentic 5’ and 3’ termini identical to those of the native B10 DI RNA, the DI RNA sequences were flanked by ribozymes (RzDI). When RzDI RNAs transcribed in vitro were mixed with parental TBSV transcripts and inoculated into protoplasts or plants, they became amplified, reduced the accumulation of the parental RNA, and mediated attenuation of the lethal syndrome characteristic of TBSV infections. Analysis of …