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Rediscovery And Redescription Of Ceradryops Punctatus Hinton, 1937 (Coleoptera: Dryopidae), P. J. Spangler, C. L. Staines

Insecta Mundi

Ceradryops punctatus Hinton horn Sri Lanka is redescribed and illustrated. Notes are made on the

Occurrence Of The Milliped Pachydesmus Crassicutis Adsinicolus Hoffman In Florida (Polydesrnida: Xystodesmidae), Rowland M. Shelley

Insecta Mundi

The xystodesmid milliped tribe Pachydesmini is represented in the southeastern states by three genera - Pachydesmus Cook, Dicellarius Chamberlin, and Thrinaxoria Chamberlin and Hoffman -eight species, and five subspecies (Hoffman 1958, 1999; Shelley 1984, 2002). The only one presently known from Florida is Dicellarius, represented by D. okefenokensis (Chamberlin), in the peninsula, and D. b. bimaculatus (McNeill) and D. b. fictus (Chamberlin) in the panhandle (Shelley 1984,2001).

Effects Of Binary Combinations Of Herbicides On Freshwater Algae, Jill K. Taylor

School of Natural Resources: Dissertations, Theses, and Student Research

No abstract provided.

Resistance Of Commercial Hybrids And Lines Of Sorghum, Sorghum Bicolor (L.) Moench., To Diatraea Saccharalis (Fabr.) (Lepidoptera: Pyralidae), Jose M. Waquil, Jose A. S. Rodrigues, Fredolino G. Santos, Alexandre S. Ferreira, Francys M. F. Vilella, John E. Foster

Department of Entomology: Faculty Publications

Commercial sorghum hybrids and Embrapa’s lines were evaluated regarding resistance to sugarcane borer. Two field experiments were conducted in Sete Lagoas, MG, under natural infestation, during the second cropping season in a randomized complete block with 3 replicates. The plants were evaluated regarding to stand, maturity cycle, plant height, and number of broken or lodging stalks and yield. The borer incidence, number of galleries, number of healthy and damaged nodes were also evaluated to calculate the Infestation Index and Intensity Infestation Index. Among the commercial sorghum hybrids, Br 304 and CMSXS 9701 were the least infested wile Z 732 and …

Chronic Exposure Of The European Corn Borer (Lepidoptera: Crambidae) To Cry1ab Bacillus Thuringiensis Toxin, Josette Chafaux, Maryvonne Seguin, Jeffrey J. Swanson, Denis Bourguet, Blair D. Siegfried

Department of Entomology: Faculty Publications

Transgenic corn expressing the insecticidal toxin from Bacillus thuringiensis Berliner is gaining support as an effective control technology for use against lepidopteran pests, particularly European corn borer, Ostrinia nubilalis Hubner (Lepidoptera: Crambidae). However, there is concern that widespread adoption of transgenic plants will rapidly lead to B. thuringiensis toxin resistance. Thus, long-term selection of O. nubilalis populations with the Cry1Ab B. thuringiensis toxin has been undertaken in several laboratories in the United States and in Europe. We present results from two independent selection experiments performed in laboratories at the University of Nebraska and at the Institut National de la Recherche …

Conceptual Constraints On Thinking About Genocide, David Moshman

Department of Educational Psychology: Faculty Publications

Our thinking is unavoidably constrained by our conceptual structures. To the extent that we reflect on and reconstruct our concepts, however, we can sharpen our thinking. The first section of this article considers the nature of concepts, highlighting a standard distinction between prototype-based and formal concepts.

Drawing on these insights from cognitive psychology, the second section suggests that the ability to recognize and understand genocides is greatly restricted by Holocaust-based conceptions of genocide. In turn one can enhance one’s understanding via the construction and application of formal concepts of genocide.

Extending this argument, I observe in the third section that …

Intracellular Signaling Molecules Activated By Epstein-Barr Virus For Induction Of Interferon Regulatory Factor 7, Luwen Zhang, Lihong Wu, Ke Hong, Joseph S. Pagano

Nebraska Center for Virology: Faculty Publications

Epstein-Barr virus (EBV) latent membrane protein 1 (LMP-1) is the principal oncogenic protein in the EBV transformation process. LMP-1 induces the expression of interferon regulatory factor 7 (IRF-7) and activates IRF-7 protein by phosphorylation and nuclear translocation. LMP-1 is an integral membrane protein with two regions in its C terminus that initiate signaling processes, the C-terminal activator regions 1 (CTAR-1) and CTAR-2. Here, genetic analysis of LMP-1 has determined that the PXQXT motif that governs the interaction between LMP-1 CTAR-1 and tumor necrosis factor receptor-associated factors (TRAFs) is needed to induce the expression of IRF-7. Mutations in the PXQXT motif …

Interferon Regulatory Factor 7: A Key Cellular Mediator Of Lmp-1 In Ebv Latency And Transformation, Luwen Zhang, Joseph S. Pagano

Nebraska Center for Virology: Faculty Publications

Interferon regulatory factor 7 (IRF-7) was cloned within the biological context of Epstein–Barr virus (EBV) latency, and has an intimate relation with EBV. EBV latent membrane protein 1 (LMP-1) regulates IRF-7 both by inducing the expression of IRF-7 and by activating IRF-7 protein through phosphorylation and nuclear translocation in a post-translational manner. The activated IRF-7 then functions to regulate both EBV and cellular target genes involved in latency, transformation and immune regulation. IRF-7 appears to be a key cellular latency protein involved in both the pathogenesis and persistence of EBV infection.

Identification Of A Cellular Protein That Interacts And Synergizes With The Rta (Orf50) Protein Of Kaposi’S Sarcoma-Associated Herpesvirus In Transcriptional Activation, Shizhen Wang, Shuhong Liu, Ming-Hoi Wu, Yunqi Geng, Charles Wood

Nebraska Center for Virology: Faculty Publications

Lytic reactivation of Kaposi’s sarcoma-associated herpesvirus (KSHV), or human herpesvirus 8, from latency requires transcriptional transactivation by the viral protein RTA encoded by the ORF50 gene. Very little is known about how RTA functions and the cellular factors that may be involved in its transactivation function. Using the yeast two-hybrid system, we have identified a human cellular protein that can interact with KSHV RTA. The cellular protein, referred to as the human hypothetical protein MGC2663 by GenBank, is encoded by human chromosome 19. This protein is 554 amino acids (aa) in size and displays sequence similarity with members of the …

Will Voluntary And Educational Programs Meet Environmental Objectives? Evidence From A Survey Of New York Dairy Farms, Gregory L. Poe, Nelson L. Bills, B. C. Bellows, Patricia Crosscombe, Richard K. Koelsch, Michael J. Kreher, Peter Wright

Department of Agricultural and Biological Systems Engineering: Faculty Publications

This paper presents the results from a survey of New York dairy farms that links manure management practices and farmer willingness to participate in voluntary environmental programs, focusing on those operations that are not automatically designated as Concentrated Animal Feeding Operations. A wide divergence is found between actual and recommended manure management practices on individual dairy farms (high), the apparent ability of farms to divert financial resources to environmental practices (mixed), and the willingness to participate in voluntary programs at various annual costs per cow (low). These findings have policy implications for the USDA/USEPA National Strategy for Animal Feeding Operations.

Predicting Risk To Biodiversity As A Function Of Aquifer Pressure In Gab Mound Springs, A. J. Tyre, Brigitte Tenhumberg, H.P. Possingham

School of Biological Sciences: Faculty Publications

Plants and aquatic invertebrates endemic to mound springs of the Great Artesian Basin (GAB) of Australia are of national biodiversity significance. Springs occur in groups, and current survey data suggest that local extinction and recolonisation within a group does occur naturally. Mound spring fauna are therefore good examples of true metapopulations, and any study of the environmental impacts on these systems must take this into account. Use of GAB water leads to drawdown of the pressure in the aquifer. This can potentially impact mound spring flora and fauna in two ways. First, as pressure decreases, so does flow from spring …

Direct Imaging Of Human Swi/Snf-Remodeled Mono- And Polynucleosomes By Atomic Force Microscopy Employing Carbon Nanotube Tips, Gavin R. Schnitzler, Chin Li Cheung, Jason H. Hafner, Andrew J. Saurin, Robert E. Kingston, Charles M. Lieber

Barry Chin Li Cheung Publications

Chromatin-remodeling complexes alter chromatin structure to facilitate, or in some cases repress, gene expression. Recent studies have suggested two potential pathways by which such regulation might occur. In the first, the remodeling complex repositions nucleosomes along DNA to open or occlude regulatory sites. In the second, the remodeling complex creates an altered dimeric form of the nucleosome that has altered accessibility to transcription factors. The extent of translational repositioning, the structure of the remodeled dimer, and the presence of dimers on remodeled polynucleosomes have been difficult to gauge by biochemical assays. To address these questions, ultrahigh-resolution carbon nanotube tip atomic …

Rumination And Cigarette Smoking: A Bad Combination For Depressive Outcomes?, Malia Richmond, Bonnie Spring, Beth Kaplan Sommerfeld, Dennis E. Mcchargue

Department of Psychology: Faculty Publications

Ruminative coping has been shown to heighten the risk and severity of depression. The authors hypothesized that ruminators who smoke would experience greater depressive symptoms than ruminators who do not. The rationale is that, by heightening attentional focus, nicotine may increase ruminators' ability to focus on negative thoughts, augmenting depressed mood. Participants (N = 145) self-reported smoking status, rumination, and current and lifetime depressive symptoms, including depressed mood. Results showed that rumination accounted for a larger amount of variance in current and past depressed mood and severity of lifetime depressive symptoms among smokers than nonsmokers. Noncorrelational, experimental research should …

Morphological And Molecular Description Of Haematoloechus Meridionalis N. Sp. (Digenea: Plagiorchioidea: Haematoloechidae) From Rana Vaillanti Brocchi Of Guanacaste, Costa Rica, Virginia León-Règagnon, Daniel R. Brooks, Derek A. Zelmer

Harold W. Manter Laboratory of Parasitology: Faculty Publications

An undescribed species of Haematoloechus inhabits the lungs of Rana vaillanti in northwestern Costa Rica. The new species is most similar morphologically to H. medioplexus, having a very small, but well-developed, ventral sucker and lacking extracecal uterine loops, and apparently was misidentified previously as H. medioplexus in Rana palmipes from Colombia. It differs from H. medioplexus, notably by (1) the shape of the oral sucker, which is elliptical in H. meridionalis and spherical in H. medioplexus; (2) the posterior extent of the vitelline fields, which terminate at the same level in H. medioplexus, but always at …

Oestrus Ovis In Sheep: Relative Third-Instar Populations, Risks Of Infection And Parasitic Control, Guillaume Tabouret, Philippe Jacquiet, Philip Scholl, Philippe Dorchies

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Oestrus ovis (L.) (Diptera: Oestridae), the nasal bot fly, has a relatively short free-living life cycle outside of the host, and therefore it is necessary to know when the parasitic period occurs in order to prevent the clinical signs and economic losses caused by this parasite. The length of this parasitic portion of the life cycle is quite variable: a few weeks to several months depending on the season and climatic conditions. Surveys of Oestrus ovis larval populations in sheep show different results on the number of generations according to the local climate. Mean monthly larval profiles of L1 and …

Ultrasound Based Quantitative Motion Measurement Using Speckle Size Estimation, James D. Hamilton, Larry Y. L. Mo, Gregory R. Bashford

Biomedical Imaging and Biosignal Analysis Laboratory

An ultrasound system determines the relative movement in a first direction (F1) of first matter, such as blood flow, and second matter, such as an artery wall, in a subject under study (S). A beam (B1) of ultrasound waves defining a plurality of beam positions (BP1 and BP2) and beam axes (A1 and A2) are moved in scan direction having components parallel to direction F1. First and second blocks of data representing the first and second matter, respectively, are generated. A processor (20) performs an estimation of speckle size on first data to obtain a first result, and performs analysis …

Identification Of Screwworms, Cochliomyia Hominivorax (Coquerel) (Diptera: Calliphoridae), With A Monoclonal Antibody-Based Enzyme-Linked Immunosorbent Assay (Mab-Elisa), J. L. Figarola, Steven R. Skoda, Dennis R. Berkebile, John E. Foster

Department of Entomology: Faculty Publications

Myiasis caused by screwworms, Cochliomyia hominivorax (Coquerel), is devastating to warmblooded animals and economically important to livestock producers. It is difficult to distinguish these pests, immature screwworms, from immatures of other non-pest fly species that often occur in animal wounds; it would be helpful to have tools available that do not rely on morphological characteristics. We developed twomonoclonal antibodies (MAbs), highly specific for the screwworm, and used them in an enzyme-linked immunosorbent assay (MAb-ELISA), that differentiated screwworm eggs, larvae, pupae, and adults from those of the closely related secondary screwworm, C. macellaria (Fabricius) as well as Phormia regina (Meigen), Phaenicia …

Nebraska Cooperative Council 56th Annual Meeting, John Owens

John Owens: Speeches and Appearances

What a pleasure it is to be with you this morning - thank you for inviting me. I am looking forward to visiting with as many of you as possible while I am here today, and hope to have the opportunity to visit with you more as we meet again at other times and other places. One of the things I am always interested in knowing is what you perceive as Nebraska's greatest needs, now and in the future, and which of those Needs you think the University of Nebraska Institute of Agriculture and Natural Resources can most efficiently and …

The Impact Of Publicly Funded Research On The Structure Of U.S. Agriculture, Jeffrey S. Royer

Cornhusker Economics

In recent years, there has been a substantial increase in the concentration of agricultural production in the United States as the number of farming and ranching operations has declined and the average size of those operations has grown. This increased concentration has been accompanied by increased coordination of production and marketing activities through contracting, consolidation and vertical integration. Although independent family farms and ranches have been responsible for most of the nation’s agricultural production historically, small and medium-sized operations are finding it difficult to compete in today’s increasingly industrialized food and agricultural sector.

Non-Repressing Carbon Sources For Alcohol Oxidase (Aoxi) Promoter Of Pichia Pastoris, Mehmet İnan, Michael M. Meagher

Papers in Biochemical Engineering

The growth of Pichia pastoris in a mixture of either glycerol or glucose and methanol follows a diauxic growth, with C1, utilizing enzymes being repressed. Therefore, these carbon sources can not be used as a mixture with methanol to simultaneously grow P. pastoris and induce C1, utilizing enzymes, especially in shake flask cultures of AOX-deficient P. pastoris. Among the alternative carbon sources tested, alanine, sorbitol, mannitol and trehalose, did not repress β-gal production when methanol was used as an inducer in mut- strain of P. pastoris. Our results show that either one of alanine, sorbitol, …

Differential Responses Of Coyotes To Novel Stimuli In Familiar And Unfamiliar Settings, Charles E. Harris, Frederick F. Knowlton

United States Department of Agriculture Wildlife Services: Staff Publications

We studied behavioral responses of captive and wild coyotes (Canis latrans) toward novel objects and artificial scent stations to determine how they might respond to novel stimuli used in coyote management and research. When captive coyotes encountered novel stimuli in unfamiliar surroundings they showed little avoidance, while the same stimuli caused avoidance and neophobic reactions among coyotes when encountered in familiar surroundings. In the field phase of the study, radio-collared coyotes in southern Texas were intensively monitored in order to relate space-use patterns to the locations where these coyotes were exposed to artificial scent stations. Coyotes were more …

The Ns5a Protein Of Bovine Viral Diarrhoea Virus Interacts With The Α Subunit Of Translation Elongation Factor-1, Craig M. Johnson, Daniel R. Perez, Roy C. French, William C. Merrick, Ruben O. Donis

Department of Plant Pathology: Faculty Publications

A cellular protein that interacts with the NS5A polypeptide of bovine viral diarrhoea virus (BVDV) was identified in a yeast two-hybrid screen. The NS5A interactor was identified as the a subunit of bovine translation elongation factor 1A (eEF1A). Cell-free binding studies were performed with chimeric NS5A fused to glutathione S-transferase (GST–NS5A) expressed in bacteria. GST–NS5A bound specifically to both in vitro-translated and mammalian cell-expressed eEF1A. Moreover, purified eEF1A bound specifically to GST–NS5A attached to a solid phase. Conservation of this interaction was then analysed using a set of NS5A proteins derived from divergent BVDV strains encompassing known biotypes and genotypes. …

The Demographic Response Of Bank-Dwelling Beavers To Flow Regulation: A Comparison On The Green And Yampa Rivers, Stewart W. Breck, Kenneth R. Wilson, Douglas C. Anderson

United States Department of Agriculture Wildlife Services: Staff Publications

We assessed the effects of flow regulation on the demography of beavers (Castor canadensis) by comparing the density, home-range size, and body size of bank-dwelling beavers on two sixth-order alluvial river systems, the flow-regulated Green River and the free-flowing Yampa River, from 1997 to 2000. Flow regulation on the Green River has altered fluvial geomorphic processes, influencing the availability of willow and cottonwood, which, in turn, has influenced the demography of beavers. Beaver density was higher on the Green River (0.5–0.6 colonies per kilometer of river) than on the Yampa River (0.35 colonies per kilometer of river). Adult …

Determinants Of Effective Population Size For Loci With Different Modes Of Inheritance, Jay F. Storz, U. Ramakrishnan, S. C. Alberts

Jay F. Storz Publications

Here we report an assessment of the determinants of effective population size (N_e) in species with overlapping generations. Specifically, we used a stochastic demographic model to investigate the influence of different life-history variables on N_e/N (where N = population census number) and the influence of sex differences in life-history variables on N_e for loci with dif¬ferent modes of inheritance. We applied an individual-based modeling approach to two datasets: one from a natural popu¬lation of savannah baboons (Papio cynocephalus) in the Amboseli basin of southern Kenya and one from a human tribal …

Modeling Bighorn Sheep Habitat In Northwest Nebraska, Kyle M. Forbes

Department of Geography: Dissertations, Theses, and Student Research

In recent years geographic information system (GIS) and remote sensing technologies have been used extensively in habitat suitability modeling. By incorporating these technologies, scientists often gain the ability to formulate and implement a more detailed habitat suitability model with the addition of more detailed spatial information. A habitat suitability model was developed to quantify and describe the available suitable Rocky Mountain bighorn sheep (Ovis canadensis canadensis) habitat in the Pine Ridge of northwestern Nebraska and determine if a minimum viable population (MVP) of this species can be supported naturally in the region. The model included a strong spatial …

Enzymological And Radiotracer Studies Of Lipid Metabolism In The Flight-Capable And Flightless Morphs Of The Wing-Polymorphic Cricket, Gryllus Firmus, Z. Zhao, Anthony J. Zera

Anthony Zera Publications

The flight-capable morph of the wing-polymorphic cricket, Gryllus firmus, exhibited significantly higher activities of each of five lipogenic enzymes compared with the obligately flightless morph on a standard and a high-carbohydrate diet during early adulthood. Similarly, the rate of incorporation of [¹⁴C]-acetate into total lipid was higher in the flight-capable morph during this time. By contrast, activities of lipogenic enzymes and rates of lipid biosynthesis, in general, did not differ between morphs on a low nutrient diet during early adulthood. Differences in lipid biosynthesis account for previously documented differences in lipid reserves between morphs on some, but …

Wpa News 65 [66] (2001), World Pheasant Association

Galliformes Specialist Group and Affiliated Societies: Newsletters

WPA News (November 2001), number 65 [66]

Published by the World Pheasant Association

Transgenic Insecticidal Corn: The Agronomic And Ecological Rationale For Its Use, Eldon E. Ortman, B. Dean Barry, Laurent L. Buschman, Dennis D. Calvin, Janet Carpenter, Galen P. Dively, John E. Foster, Billy W. Fuller, Richard L. Hellmich, Randall A. Higgens, Thomas E. Hunt, Gary P. Munkvold, Kenneth R. Ostlie, Marlin E. Rice, Richard T. Rousch, Mark K. Sears, Anthony M. Shelton, Blair D. Siegfried, Phillip E. Sloderbeck, Kevin L. Steffey, F. Tom Turpin, John L. Wedberg

Department of Entomology: Faculty Publications

We agree with Obrycki et al. (2001) that a broad-based ecological approach for new pest management technologies is desirable, but we unanimously and strongly disagree with some of their assumptions and conclusions about Bt corn. Bt corn is corn that has been genetically engineered to produce insecticidal proteins from the bacterium Bacillus thuringiensis. Because Bt corn is important for effective and ecologically sound management of lepidopteran pests of corn,we provide here relevant data, some of which is new, to help clarify the issues raised by Obrycki et al. (2001). Obrycki et al. (2001), citing Barry and Darrah (1991), claim that …

Structure And Temporal Dynamics Of Populations Within Wheat Streak Mosaic Virus Isolates, Jeffrey S. Hall, Roy C. French, Thomas Jack Morris, Drake C. Stenger

Department of Plant Pathology: Faculty Publications

Variation within the Type and Sidney 81 strains of wheat streak mosaic virus was assessed by single-strand conformation polymorphism (SSCP) analysis and confirmed by nucleotide sequencing. Limiting-dilution subisolates (LDSIs) of each strain were evaluated for polymorphism in the P1, P3, NIa, and CP cistrons. Different SSCP patterns among LDSIs of a strain were associated with single-nucleotide substitutions. Sidney 81 LDSI-S10 was used as founding inoculum to establish three lineages each in wheat, corn, and barley. The P1, HC-Pro, P3, CI, NIa, NIb, and CP cistrons of LDSI-S10 and each lineage at passages 1, 3, 6, and 9 were evaluated for …

Structure And Temporal Dynamics Of Populations Within Wheat Streak Mosaic Virus Isolates, Jeffrey S. Hall, Roy C. French, Thomas Jack Morris, Drake C. Stenger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Variation within the Type and Sidney 81 strains of wheat streak mosaic virus was assessed by single-strand conformation polymorphism (SSCP) analysis and confirmed by nucleotide sequencing. Limiting-dilution subisolates (LDSIs) of each strain were evaluated for polymorphism in the P1, P3, NIa, and CP cistrons. Different SSCP patterns among LDSIs of a strain were associated with single-nucleotide substitutions. Sidney 81 LDSI-S10 was used as founding inoculum to establish three lineages each in wheat, corn, and barley. The P1, HC-Pro, P3, CI, NIa, NIb, and CP cistrons of LDSI-S10 and each lineage at passages 1, 3, 6, and 9 were evaluated for …