Digital Commons Network^™

Single-Nucleotide Polymorphisms In Soybean, Y. L. Zhu, Q. J. Song, D. L. Hyten, C. P. Van Tassel, L. K. Matukumalli, D. R. Grimm, S. M. Hyatt, E. W. Fickus, N. D. Young, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and random genomic sequence; (2) to characterize haplotype variation in these sequences; and (3) to provide initial estimates of linkage disequilibrium (LD) in soybean. Approximately 28.7 kbp of coding sequence, 37.9 kbp of noncoding perigenic DNA, and …

Actual Innocents: Considerations In Selecting Cases For A New Innocence Project, Daniel S. Medwed

Nebraska Law Review

I. Introduction . . . . . 1098

II. Practical Considerations . . . . . 1102

A. Defining Your Scope: Determining What Kinds of Cases You Want . . . . . 1103

1. Substance: Limitations on Case Content . . . . . 1104

2. Form: Procedural, Jurisdictional, and Other Limitations . . . . . 1109

B. Putting Theory into Practice: How Do You Get the Cases You Want? . . . . . 1114

1. Inflow: Gathering Prospective Innocence Claims . . . . . 1114

2. Outflow: Procedures for Evaluating Potential Cases . . . …

"What's Love Got To Do With It?" "It's Not Like They're Your Friends For Christ's Sake": The Complicated Relationship Between Lawyer And Client, Robert J. Condlin

Nebraska Law Review

Should lawyers love their clients and try to be their friends? Scholars have defended the “lawyer-as-friend” analogy, but usually they have relied on a more contractual understanding of friendship compatible with traditional concepts of adversary advocacy. I will discuss lawyer-client relationships by examining two generations of scholarly argument for the lawyer-as-friend view, and a description of the approach in operation in an actual legal case. These discussions show in detail what sorts of questions a lawyer-as-friend takes up, how he or she divides or shares representational tasks with clients differently from lawyers who use more traditional approaches to legal representation, …

Indirect Evidence Of Density-Dependent Population Regulation In Aponomma Hydrosauri (Acari: Ixodidae), An Ectoparasite Of Reptiles, Andrew J. Tyre, C. Michael Bull, Brigitte Tenhumberg, Neil Chilton

School of Natural Resources: Faculty Publications

The extent to which density-dependent processes regulate natural populations is the subject of an ongoing debate. We contribute evidence to this debate showing that density-dependent processes influence the population dynamics of the ectoparasite Aponomma hydrosauri (Acari: Ixodidae), a tick species that infests reptiles in Australia. The first piece of evidence comes from an unusually long-term dataset on the distribution of ticks among individual hosts. If density-dependent processes are influencing either host mortality or vital rates of the parasite population, and those distributions can be approximated with negative binomial distributions, then general host– parasite models predict that the aggregation coefficient of …

U.S. Fish And Wildlife Service 2003 Amendment To The 2000 Biological Opinion On The Operation Of The Missouri River Main Stem Reservoir System, Operation And Maintenance Of The Missouri River Bank Stabilization And Navigation Project, And Operation Of The Kansas River Reservoir System

United States Army Corps of Engineers, Omaha District

In 1989, the U.S. Army Corps of Engineers (Corps) initiated consulation with the U.S. Fish and Wildlife Service (Service) regarding operation of the Missouri River Main Stem Reservoir System.  The species covered were the endangered Interior least tern (Sterna antillarum), threatened Northern Great Plains piping plover (Charadrius melodus), and the endangered bald eagle (Hallaeetus leucocehpalus).  Subsequently, the pallid sturgeon (Seaphirhynchus alba) was listed as endangered in 1990.  The bald eagle is now a threatened species proposed for delisting.

Consultation between the Corps and the Service in 2000 covered operations of the Missouri …

Implementation Of The Biological Opinion For The Missouri River Mainstem System, Missouri River Bank Stabilization And Navigation Project, And Kansas River Reservoir System

United States Army Corps of Engineers, Omaha District

The Endangered Species Act (ESA) requires that the Corps of Engineers (Corps), in coordination with the appropriate resource agencies, will ensure that any action authorized, funded, or carried out is not likely to jeopardize the continued existence of any federally listed threatened or endangered species or result in the destruction or adverse modification of critical habitat. On November 30, 2000, formal consultation between the U.S. Fish and Wildlife Service (USFWS) and the Corps under Section 7 of the ESA culminated with the "Biological Opinion on the Operation of the Missouri River Main Stem System, Operation and Maintenance ofthe Missouri River …

Alteration Of Soil Water Content Consequent To Root-Pruning At A Windbreak/Crop Interface In Nebraska, Usa, Qingjiang Hou, James R. Brandle, Kenneth Hubbard, Michele Schoneberger, Carlos Nieto, Charles A. Francis

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Root-pruning is generally recommended as an appropriate treatment to reduce competition for soil water and/or nutrients and suppression of crop yield in areas adjacent to windbreaks. Several recent studies suggest, however, that factors other than soil water might be causing yield reduction at the interface. For two consecutive years, we evaluated root-pruning effects on soil water at the windbreak/crop interface under both cropped (soybean [Glycine max (L) Merr.] variety ‘Iroquois’, 1997) and non-cropped (1998) conditions in Mead, Nebraska, USA. Volumetric soil water content near the windbreaks was systematically measured at various soil depths, distances from the windbreak, …

Use Of Mini-Sprinklers To Strip Trichloroethylene And Tetrachloroethylene From Contaminated Ground Water, Yvette C. Berisford, Parshall B. Bush, John I. Blake, Cassandra L. Bayer

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Three low-volume mini-sprinklers were tested for their efficacy to strip trichloroethylene (TCE) and tetrachloroethylene (PCE) from water. Deionized water spiked with TCE and PCE as pumped for approximately 1 h at 0.19 to 0.21 MPa (28 to 30 lb in^-2) through a mini-sprinkler supported on top of a 1.8-m-tall riser. Water was collected in collection vessels at 0.61 and 1.22 m above the ground on support columns that were spaced at 0.61-m intervals from the riser base, and samples were composited per height and distance from the riser. Overall, air-stripping reduced dissolved concentrations of TCE and PCE by …

Native Weeds And Exotic Plants: Relationships To Disturbance In Mixed-Grass Prairie, Diane L. Larson

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Disturbance frequently is implicated in the spread of invasive exotic plants. Disturbances may be broadly categorized as endogenous (e.g., digging by fossorial animals) or exogenous (e.g., construction and maintenance of roads and trails), just as weedy species may be native or exotic in origin. The objective of this study was to characterize and compare exotic and native weedy plant occurrence in and near three classes of disturbance – digging by prairie dogs (an endogenous disturbance to which native plants have had the opportunity to adapt), paved or gravel roads (an exogenous disturbance without natural precedent), and constructed trails (an exogenous …

Effects Of Ungulates And Prairie Dogs On Seed Banks And Vegetation In A North American Mixed-Grass Prairie, Jace T. Fahnestock, Diane L. Larson, Glenn E. Plumb, James K. Detling

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The relationship between vegetation cover and soil seed banks was studied in five different ungulate herbivoreprairie dog treatment combinations at three northern mixed-grass prairie sites in Badlands National Park, South Dakota. There were distinct differences in both the seed bank composition and the aboveground vegetation between the off-prairie dog colony treatments and the on-colony treatments. The three on-colony treatments were similar to each other at all three sites with vegetation dominated by the forbs Dyssodia papposa, Hedeoma spp., Sphaeralcea coccinea, Conyza canadensis, and Plantago patagonica and seed banks dominated by the forbs Verbena bracteata and Dyssodia papposa. The …

Socialization Of Boys And Girls In Natural Contexts, Carolyn P. Edwards, Lisa Knoche, Asiye Kumru

Department of Psychology: Faculty Publications

Socialization is the general process by which the members of a cultural community or society pass on their language, rules, roles, and customary ways of thinking and behaving to the next generation. Sex role socialization is one important aspect of this general process. The goals of earlier work were to understand how, why, and at what age girls and boys begin to vary behaviorally along such dimensions as "nurturance," "aggression," and "dependency," including determination of how sex-typical dispositions are influenced by cultural factors. This chapter presents a new approach seeking to answer such questions as the following. How are different …

Bibliography, Volume 21 (2003)

To Improve the Academy: A Journal of Educational Development

Bibliography for volume 21 (2003) of To Improve the Academy: A Journal of Educational Development.

United States Patent Application Publication: Divide And Conquer System And Method Of Dna Sequence Assembly, Khalid Sayood, Hasan H. Otu

Department of Electrical and Computer Engineering: Faculty Publications

The present invention provides a new algorithm for assembling fragments from a long DNA sequence. The algorithm of the invention Solves simultaneously fragment assembly-related problems. Such as the fragment orientation, overlap and layout phases. This is achieved by clustering fragments with respect to their Average Mutual Information (AMI) profiles using the k-means algorithm.

Mississippi River Blufflands And Bird Conservation, Eileen M. Kirsch

United States Geological Survey: Staff Publications

The "rubber hits the road" in conservation when the landowning public involved. Researchers and amateur birders know that populations of many species of neotropical migrant birds are declining because of habitat loss and many natural- and human-caused dangers. However, the landowners may not know what neotropical migrants are or that they are in peril. This excellent booklet and enclosed pamphlet are intended to educate landowners along the Mississippi River blufflands in Iowa, Illinois, Wisconsin, and Minnesota about neotropical migrant birds and their plight. Furthermore, it tactfully attempts to inspire concern about neotropical migrants and explains how management can help. While …

Seasonal Distribution Of Migratory Tree Bats (Lasiurus And Lasionycteris) In North America, Paul M. Cryan

United States Geological Survey: Staff Publications

Despite evidence of migration in North American tree bats (genera Lasiurus and Lasionycteris), details regarding seasonal movements in these widely distributed species are few. This study attempted to clarify patterns of seasonal distribution by mapping museum occurrence records by month. Monthly changes in the distribution of records indicate seasonal movements of tree bats. Northward migration during spring by Lasiurus cinereus appears to stem from wintering grounds in California and Mexico. During summer, male L. cinereus is mainly distributed in western North America, whereas females dominate samples from eastern regions. Lasiurus borealis winters in southeastern areas and expands its range …

Evaluation Of Landscape Models For Wolverines In The Interier Northwest, United States Of America, Mary M. Rowland, Michael J. Wisdom, Douglas H. Johnson, Barbara C. Wales, Jeffrey P. Copeland, Frank B. Edelmann

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The wolverine (Gulo gulo) is an uncommon, wide-ranging carnivore of conservation con- cern. We evaluated performance of landscape models for wolverines within their historical range at 2 scales in the interior Northwest based on recent observations (n = 421) from Washington, Oregon, Idaho, and Montana. At the sub-basin scale, simple overlays of habitat and road-density classes were effective in predicting observations of wolverines. At the watershed scale, we used a Bayesian belief network model to provide spatially explicit estimates of relative habitat capability. The model has 3 inputs: amount of habitat, human population density, and road density. …

Potentiation Of Trail-Induced Apoptosis In Primary Effusion Lymphoma Through Azidothymidine-Mediated Inhibition Of Nf-[Kappa]B, Subrata K. Ghosh, Charles Wood, Lawrence H. Boise, Abdul M. Mian, Vadim V. Deyev, Gerold Feuer, Ngoc L. Toomey, Nicole C. Shank, Lisa Cabral, Glen N. Barber, William J. Harrington

Nebraska Center for Virology: Faculty Publications

The survival of viral mediated lymphomas depends upon constitutive nuclear factor kappa B (NF-[kappa]B) activity. AIDS-related human herpesvirus type 8–associated primary effusion lymphoma (PEL) responds poorly to chemotherapy and is almost invariably fatal. We have previously demonstrated that the antiviral combination of interferon alpha (IFN-[alpha]) and azidothymidine (AZT) induces apoptosis in PEL cell lines. We therefore used these agents as therapy for an AIDS patient with PEL. The patient had a dramatic response, with complete resolution of his malignant effusion in 5 days. In PEL cells, the death receptor ligand known as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is markedly …

Low Fluence, Robert Katz, F. A. Cucinotta

Robert Katz Publications

The question of the appropriate extrapolation to low dose has long been a subject of controversy. A linear no-threshold model is favored by regulatory bodies as the basis of RBE assignments and estimates of radiation hazards to the general population. This model is largely supported by extensive application of the linear-quadratic survival formula "fitted" statistically to a wide variety of experimental data obtained at doses typically exceeding 1 Gy, and then extrapolated to mGy for practical applications, and even to the prediction of hazards from single electrons. Such extrapolations are questionable at best, and may even prove hazardous for risk …

Book Notes- Winter 2003

Great Plains Quarterly (through 2013)

Book Notes

Niddrie of the North-West: Memoirs of a Pioneer Canadian Missionary

A Prairie Mosaic: An Atlas of Central Nebraska's Land, Culture and Nature

The Oregon Trail: A Photographic Journey

Canyons of the Texas High Plains

Dictionary of Midwestern Literature. Volume One: The Authors

The University of Manitoba: An Illustrated History

Willa Cather: The Contemporary Reviews

Identification Of Novel Domains Within Sox-2 And Sox-11 Involved In Autoinhibition Of Dna Binding And Partnership Specificity, Matthew S. Wiebe, Tamara K. Nowling, Angie Rizzino

Nebraska Center for Virology: Faculty Publications

Sox transcription factors play key regulatory roles throughout development, binding DNA through a consensus (A/T)(A/T)CAA(A/T)G sequence. Although many different Sox proteins bind to this se-quence, it has been observed that gene regulatory elements are commonly responsive to only a small subset of the entire family, implying that regulatory mechanisms exist to permit selective DNA bind-ing and/or transactivation by Sox family members. To identify and explore the mechanisms modu-lating gene activation by Sox proteins further, we compared the function of Sox-2 and Sox-11. This led to the discovery that Sox proteins are regulated differentially at multiple levels, including trans-activation, protein partnerships …

Wolves: Behavior, Ecology, And Conservation: Photo Section I, Douglas W. Smith, Rolf Peterson, L. David Mech, Barron Crawford

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Wolves in Yellowstone National Park's restored population often must compete with grizzly bears at the carcasses of their kills. Especially after their first feeding at a kill, wolves tend to relinquish their kills to bears. Photograph by Doug Smith.

Elk are one of the most common prey of wolves in the northern Rocky Mountains of North America, primarily because in most areas they tend to outnumber other prey. This large bull standing his ground escaped this pack. Photograph by Doug Smith.

Incidence Of Mink, Mustela Vison, And River Otter, Lutra Canadensis, In A Highly Urbanized Area, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Mink (Mustela vison) frequently inhabited or traversed a residential. business. and industrial part of the Twin Cities. Minnesota, with little water or natural vegetation. At least one River Otter (Lutra canadensis) also resided on a small pond on a golf course in the area for several winter months.

Eutrophication And Carbon Sources In Chesapeake Bay Over The Last 2700 Yr: Human Impacts In Context, John F. Bratton, Steven M. Colman, Robert R. Seal, Ii

United States Geological Survey: Staff Publications

To compare natural variability and trends in a developed estuary with human-influenced patterns, stable isotope ratios (δ¹³C and δ¹⁵N) were measured in sediments from five piston cores collected in Chesapeake Bay. Mixing of terrestrial and algal carbon sources primarily controls patterns of δ¹³C_org profiles, so this proxy shows changes in estuary productivity and in delivery of terrestrial carbon to the bay. Analyses of δ¹⁵N show periods when oxygen depletion allowed intense denitrification and nutrient recycling to develop in the seasonally stratified water column, in addition to recycling taking place in surficial …

Structural Analyses Of Phycodnaviridae And Iridoviridae, Alan A. Simpson, Narayanasamy Nandhagopal, James L. Van Etten, Michael G. Rossmann

Nebraska Center for Virology: Faculty Publications

The Phycodnaviridae, Iridoviridae and related viruses, with diameters of 1500±2000 A Ê , are formed from large trigonal arrays of hexagonally close-packed capsomers forming the faces of icosahedra [Yan et al. (2000), Nature Struct. Biol. 7, 101-103; Nandhagopal et al. (2002), Proc. Natl Acad. Sci. USA, 99, 14758-14763]. Caspar and Klug predicted that such structures could be assembled from hexameric capsomers [Caspar & Klug (1962), Cold Spring Harbor. Symp. Quant. Biol. 27, 1-24], as was subsequently found in numerous icosahedral viruses. During the course of evolution, some viruses, including the virus families …

Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention provides a method to detect the presence of a microorganism or agent in an animal. The method encompasses placement of devices at various locations where the animal resides so as to induce the animal to initiate contact with the device. As a result of this contact, the animal deposits various microorganisms and agents on the device. The device is then tested for the presence of the particular microorganism or agent of interest.

Calibration Of Remotely Sensed, Coarse Resolution Ndvi To Co2 Fluxes In A Sagebrush–Steppe Ecosystem, Bruce K. Wylie, Douglas A. Johnson, Emilio Laca, Nicanor Z. Saliendra, Tagir G. Gilmanov, Bradley C. Reed, Larry L. Tieszen, Bruce B. Worstell

United States Geological Survey: Staff Publications

The net ecosystem exchange (NEE) of carbon flux can be partitioned into gross primary productivity (GPP) and respiration (R). The contribution of remote sensing and modeling holds the potential to predict these components and map them spatially and temporally. This has obvious utility to quantify carbon sink and source relationships and to identify improved land management strategies for optimizing carbon sequestration. The objective of our study was to evaluate prediction of 14-day average daytime CO₂ fluxes ( F_day) and nighttime CO₂ fluxes (R_n) using remote sensing and other data. F_{day …}

Use Of Radar Remote Sensing (Radarsat) To Map Winter Wetland Habitat For Shorebirds In An Agricultural Landscape, Oriane W. Taft, Susan M. Haig, Chris Kiilsgaard

United States Geological Survey: Staff Publications

Many of today’s agricultural landscapes once held vast amounts of wetland habitat for waterbirds and other wildlife. Successful restoration of these landscapes relies on access to accurate maps of the wetlands that remain. We used C-band (5.6-cm-wavelength), HH-polarized radar remote sensing (RADARSAT) at a 38° incidence angle (8-m resolution) to map the distribution of winter shorebird (Charadriiformes) habitat on agricultural lands in the Willamette Valley of western Oregon. We acquired imagery on three dates (10 December 1999, 27 January 2000, and 15 March 2000) and simultaneously collected ground reference data to classify radar signatures and evaluate map accuracy …

Capture Of Breeding And Wintering Shorebirds With Leg-Hold Noose-Mats, Katherine R. Mehl, Kiel L. Drake, Gary W. Page, Peter M. Sanzenbacher, Susan M. Haig, Jonathan E. Thompson

United States Geological Survey: Staff Publications

Development of effective trapping techniques is important for conservation efforts, as marking and subsequent monitoring of individuals is necessary to obtain accurate estimates of demography, movements, and habitat use. We describe a leg-hold noose-mat trap for capturing breeding and nonbreeding shorebirds. Using this method, we trapped 50 Piping Plovers (Charadrius melodus), 2258 Snowy Plovers (C. alexandrinus), 38 Killdeers (C. vociferus), and 64 Dunlins (Calidris alpina) in the western and southern United States. The trap was lightweight, making it easy to transport and set up. It was effective on unvegetated substrates at both …

Longitudinal Analysis Of Bioaccumulative Contaminants In Freshwater Fishes, Jianguo Sun, C. J. Schmitt

United States Geological Survey: Staff Publications

The National Contaminant Biomonitoring Program (NCBP) was initiated in 1967 as a component of the National Pesticide Monitoring program. It consists of periodic collection of freshwater fish and other samples and the analysis of the concentrations of persistent environmental contaminants in these samples. For the analysis, the common approach has been to apply the mixed two-way ANOVA model to combined data. A main disadvantage of this method is that it cannot give a detailed temporal trend of the concentrations since the data are grouped. In this paper, we present an alternative approach that performs a longitudinal analysis of the information …

Roles Of Mycobacterium Smegmatis D-Alanine:D-Alanine Ligase And D-Alanine Racemase In The Mechanisms Of Action Of And Resistance To The Peptidoglycan Inhibitor D-Cycloserine, Zhengyu Feng, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

D-Cycloserine (DCS) targets the peptidoglycan biosynthetic enzymes D-alanine racemase (Alr) and Dalanine: D-alanine ligase (Ddl). Previously, we demonstrated that the overproduction of Alr in Mycobacterium smegmatis determines a DCS resistance phenotype. In this study, we investigated the roles of both Alr and Ddl in the mechanisms of action of and resistance to DCS in M. smegmatis. We found that the overexpression of either the M. smegmatis or the Mycobacterium tuberculosis ddl gene in M. smegmatis confers resistance to DCS, but at lower levels than the overexpression of the alr gene. Furthermore, a strain overexpressing both the alr and ddl …