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Heritability Of Intramammary Infections At First Parturition And Relationships With Sire Transmitting Abilities For Somatic Cell Score, Udder Type Traits, Productive Life, And Protein Yield, D. L. Nash, G. W. Rogers, J. B. Cooper, G. L. Hargrove, Jeffrey F. Keown

Department of Animal Science: Faculty Publications

The objective of this study was to determine the relationships among daughter intramammary infections at first parturition and sire transmitting abilities for somatic cell score, udder type traits, productive life, and protein yield. Quarter milk samples from 958 daughters (in eight Pennsylvania herds and one Nebraska herd) of 182 Holstein sires were collected within a few days of first calving and cultured to determine intramammary infection status. A total of 446 cows had intramammary infections in 835 quarters at first parturition. Incidence of intramammary infections at first parturition and the proportion of quarters infected per cow were regressed on age …

Effect Of Chronic Treatment With The Gonadotrophin-Releasing Hormone Agonist Azagly-Nafarelin On Basal Concentrations Of Lh In Prepubertal Bulls, H. Jiménez-Severiano, M. J. D'Occhio, D. D. Lunstra, M. L. Mussard, J. W. Koch, L. R. Ehnis, W. J. Enright, J. E. Kinder

Department of Animal Science: Faculty Publications

Administration of GnRH agonist for an extended period inhibits pulsatile LH release but enhances testicular function of bulls. The mechanism whereby long-term administration of GnRH agonist enhances testosterone concentration in the blood of bulls has not been determined. The aim of this study was to determine whether chronic treatment with the GnRH agonist, azagly-nafarelin, increases blood concentrations of LH and FSH in prepubertal bulls. Two different doses of the GnRH agonist were administered via Alzet mini-osmotic pumps for 28 days. Blood samples were collected at 20 min intervals for 24 h at days 2, 13 and 25 of treatment. Agonist-treated …

The Effect Of Corn Silage Particle Size And Cottonseed Hulls On Cows In Early Lactation, P. J. Kononoff, A. J. Heinrichs

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the effects of reducing forage particle length (FPL) and the inclusion of cottonseed hulls (CSH) on intake, digestibility, chewing activity, and milk production of cows in early lactation. Sixteen multiparous cows averaging 17 ± 3 d in milk and 677 ± 58 kg BW were assigned to one of four 4 × 4 Latin squares. One square contained ruminally cannulated cows to evaluate effects of treatment on rumen fermentation and function. During each of the 23-d periods, cows were offered one of four total mixed rations that differed in particle length (long …

The Ability Of The N-Alkane Technique To Estimate Intake And Diet Choice Of Sheep, R. M. Lewis, A. M. Magadlela, N. S. Jessop, G. C. Emmans

Department of Animal Science: Faculty Publications

This study assessed the efficacy of the n-alkane technique to estimate intake and diet composition in animals given single foods or a choice of two. In thefirst experiment intakes of pelleted ryegrassand lucerne, given eitheralone or as a choice, were measured in lambshoused indoors in individual pens. Each of the three feeding treatments was given to 12 lambs at two degrees of maturity (0.30 and 0·45 of estimated mature sizes). The 12 lambs were constituted as three replicates of the two sexes of each of two breeds. The measured intakes werecompared with those estimated using the n-alkanes C31 andC33, found …

Chemotactic Role Of Neurotropin 3 In The Embryonic Testis That Facilitates Male Sex Determination, Andrea S. Cupp, Mehmet Uzumcu, Michael F. Skinner

Department of Animal Science: Faculty Publications

The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to differentiate and have been shown to express neurotropin-3 (NT3), which can act on highaffinity trkC receptors expressed on migrating mesonephros cells. NT3 expression is elevated in the embryonic testis during the time of seminiferous cord formation. A trkC receptor tyrophostin inhibitor, AG879, was found to inhibit seminiferous cord formation and mesonephros cell migration. Beads containing NT3 were found to directly …

G03-1512 Establishing Conservation Plantings Of Nut Trees And Shrubs By Direct Seeding Methods, Steven D. Rasmussen, Scott J. Josiah, John Wilson

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide explains how to plant and raise nut trees and shrubs from seed. Seeding methods, planting design, site preparation and post-planting care are discussed.

Conservation tree and shrub plantings create wildlife habitat, improve water quality, produce marketable nuts, and enhance landscape diversity. Usually, nursery-grown seedlings are used to establish these plantings. However, a number of problems exist when using seedlings, including increased costs and reduced initial tree vigor and survival. Direct seeding of tree and shrub seeds avoids many of these problems, and is a proven, effective means to establish conservation plantings in Nebraska and elsewhere.

References For The Private, The Public, And The Published: Reconciling Private Lives And Public Rhetoric, Barbara Couture, Thomas Kent

Department of English: Faculty Publications

Approximately 325 bibliographical references (15 pages) on public intrusions into private lives.

Ec03-177 Noxious Weeds Of Nebraska: Purple Loosestrife, Stevan Z. Knezevic

University of Nebraska-Lincoln Extension: Historical Materials

Purple loosestrife is an introduced invasive weed that is overrunning thousands of acres of wetlands and waterways in the Midwest. Once purple loosestrife invades a wetland, natural habitat is lost and the productivity of native plant and animal communities is severely reduced. This Extension Circular discusses the biology, identification, distribution and control of purple loosestrife.

G03-1522 Damping Off Of Seedlings And Transplants, Laurie Hodges

University of Nebraska-Lincoln Extension: Historical Materials

The shift toward more ecological means of pest control is a driving force in research and in growers' fields. Growers need to understand how the various pathogens causing plant disease respond to environmental conditions and cultural practices — what keeps the level of disease below an economic threshold and how these conditions can be developed and maintained for more sustainable production.

This NebGuide provides information on two common pathogens that cause seedling disease (damping off) in many crops. Although vegetable production is used in the examples, the principles apply to many cropping systems.

G03-1527 Annual Forages For The Nebraska Panhandle, Burt A. Weichenthal, David D. Baltensperger, K. P. Vogel, S. D. Masterson, J.M. Blumenthal, J.M. Krall

University of Nebraska-Lincoln Extension: Historical Materials

Annual forages have value as pasture, green chop, silage, and hay. With adequate soil moisture, these forages grow very rapidly during their appropriate growing seasons. Advantages and disadvantages of various annual forages, results of dryland and irrigated forage trials, tips for achieving quality forage, and issues related to animal health and feeding forages are discussed in this NebGuide.

2002-2003 Annual Report

Lancaster County, Nebraska Extension: Reports

Extension Provides Reliable, Research-Based Information New to Extension in 2003 Pioneering New Frontiers Local Extension Programs Attract Regional, National & International Attention Web Site Has Over 3,750 Pages, 2.5 Million Hits Annually UNL Cooperative Extension in Lancaster County 2002-03 Honors and Awards About the Extension Board Helping Every Time People Eat! Limited-Income Residents Stay Healthy Through Nutrition Education Program Spotlight on Extending Program Outreach: Cooking with Spices & Herbs Promoting Healthy and Safe Homes Extension Builds Strong Families, Communities Family and Community Education Clubs The Buzz on Controlling Insects and Other Pests Termite Workshops Help Consumers Make Better Decisions Plant …

G1512 Establishing Conservation Plantings Of Nut Trees And Shrubs By Direct Seeding Methods, Steven D. Rasmussen, Scott J. Josiah, Jon Wilson

University of Nebraska-Lincoln Extension: Historical Materials

This NebGuide explains how to plant and raise nut trees and shrubs from seed. Seeding methods, planting design, site preparation and post-planting care are discussed.

Introduction

Conservation tree and shrub plantings create wildlife habitat, improve water quality, produce marketable nuts, and enhance landscape diversity. Usually, nursery-grown seedlings are used to establish these plantings. However, a number of problems exist when using seedlings, including increased costs and reduced initial tree vigor and survival. Direct seeding of tree and shrub seeds avoids many of these problems, and is a proven, effective means to establish conservation plantings in Nebraska and elsewhere.

Summary

Conservation …

Ec03-1567 Grasshopper Identification Guide For Rangeland Grasshoppers Fall/Spring Feeding Species, Ron Seymour, Jack Campbell, Gary Hein

University of Nebraska-Lincoln Extension: Historical Materials

Extension Circular 03-1567: Grasshopper Identification Guide for Rangeland Grasshoppers Fall/Spring Feeding Species; facts, adult identification, and immature identification of Specklewinged grasshopper, Redshanked grasshopper, Velvetstriped grasshopper, and Threebanded grasshopper. Biology, management, and nymph development.

Ec02-130-D Revised 2003 2003 Guide For Weed Management In Nebraska

University of Nebraska-Lincoln Extension: Historical Materials

Extension Circular 02-130-D revised 2003: Guide for Weed Management. This book from 2003 goes through various techniques and strategies for keeping crops weed free.

Megapode Newsletter 17(1)-21(2) (2003-2008), World Pheasant Association, Birdlife International, Ssc Megapode Specialist Group

Galliformes Specialist Group and Affiliated Societies: Newsletters

Volume 17, Number 1 (April 2003)

Editors: René W. R. J. Dekker and Darryl N. Jones

Viable population of Bruijn's Brush-turkey Aepypodius bruijnii discovered, Iwein Mauro

A new, a forgotten and a lost specimen of Bruijn's Brush-turkey Aepypodius bruijnii, C. W. Moeliker, C. J. Heij, J. N. J. Post, and E. J. O. Kompanje

Successful synanthropy in an Australian megapode, Darryl N. Jones

Monitoring the Nicobar megapode Megapodius nicobariensis: Phase I, Current status and conservation of the Nicobar megapode Megapodius nicobariensis, K. Sivakumar

Megapode Genetics Project proposal, Gillian C. Baker

Polynesian megapode Megapodius pritchardii status survey on …

Scale-Up Of The Fermentation And Purification Of The Recombinant Heavy Chain Fragment C Of Botulinum Neurotoxin Serotype F, Expressed In Pichia Pastoris, Scott K. Johnson, Wenhui Zhang, Leonard A. Smith, Karen J. Hywood-Potter, S. Todd Swanson, Vicki L. Schlegel, Michael M. Meagher

Michael Meagher Publications

A recombinant heavy chain fragment C of botulinum neurotoxin serotype F (BoNTF(Hc)) has been expressed in Pichia pastoris for use as an antigen in a proposed human vaccine. P. pastoris cells were grown using glycerol batch, glycerol fed-batch, and methanol fedbatch methods to achieve high cell densities. The total cellular protein recovered after homogenization was 72 mg/g of cell paste. BoNTF(Hc) was purified from soluble Pichia cell lysate employing ion-exchange chromatographic (IEC) and hydrophobic interaction chromatographic (HIC) methods developed at the bench scale using 10–100mL columns. The process was performed at the pilot scale using 1–4 L columns for evaluation …

The Formin-Homology-Domain-Containing Protein Fhod1 Enhances Cell Migration, Sreenivas Koka, Cheryl L. Neudauer, Xiaodong Li, Robert E. Lewis, James B. Mccarthy, Jennifer J. Westendorf

College of Dentistry: Faculty Publications

Formin-homology-domain-containing proteins interact with Rho-family GTPases and regulate actin cytoskeleton organization and gene transcription. FHOD1 is a member of this family, interacts with Rac1 and induces transcription from the serum response element. In this study, we examined the effects of FHOD1 expression on cytoskeletal organization and function in mammalian cells. FHOD1 proteins were stably expressed in WM35 melanoma cells and NIH-3T3 fibroblasts. Cells expressing full-length FHOD1 demonstrated an elongated phenotype compared with vector-transfected cells and cells expressing a truncated FHOD1 (1-421) that lacks the conserved FH1 and FH2 domains. Full-length FHOD1 co-localized with filamentous actin at cell peripheries. Cells transiently …

Kairomonal Effect Of Walking Traces From Euschistus Heros (Heteroptera: Pentatomidae) On Two Strains Of Telenomus Podisi (Hymenoptera: Scelionidae), Miguel Borges, Stefano Colazza, Pamela Ramirez-Lucas, Kamlesh R. Chauhan, Maria Carolina Blassioli Moraes, Jeffrey Richard Aldrich

Entomology Papers from Other Sources

The semiochemical cues used by geographically isolated strains of the parasitoid, Telenomus podisi (Ashmed), to find eggs of the stink bug Euschistus heros were investigated.

Effects Of Sucrose Concentrations And Fly Age On Feeding Responses And Survival Of Female And Male Western Cherry Fruit Flies, Rhagoletis Indifferens, Wee L. Lee

Entomology Papers from Other Sources

The effects of single meals of different sucrose concentrations on feeding responses and survival of 8–24-h-old, 1–2-, 10–12- and 31–36-day-old female and male western cherry fruit flies, Rhagoletis indifferens Curran, were determined. Feeding time and food consumption response patterns in both sexes within age groups were curvilinear. Feeding times increased as sucrose concentrations increased, and were longest when the sucrose concentration was 100% (dry). Consumption of dilute wet sucrose was low, whereas consumption of concentrated wet sucrose was high. However, consumption of dry, 100%sucrose was also low. One to 2-day-old flies of both sexes that had not previously fed consumed …

Ec03-702 Precision Agriculture: Applications Of Remote Sensing In Site-Specific Management, Viacheslav I. Adamchuk, Richard L. Perk, James S. Schepers

University of Nebraska-Lincoln Extension: Historical Materials

Precision farming is an emerging agricultural technology that involves managing each crop input on a site-specific basis to reduce waste, increase profits, and maintain the quality of the environment. Remote sensing is a technology that can be used to obtain various spatial layers of information about soil and crop conditions. It allows detection and/or characterization of an object, series of objects, or landscape without having the sensor in physical contact.

Mp82 2003-2004 Nebraska Dairy Report

University of Nebraska-Lincoln Extension: Historical Materials

Annual Report of University of Nebraska-Lincoln Dairy Research for 2003-2004. Topics include:

Lactation Curves for Milk, Fat and Protein Yields and Somatic Cell Scores of Holstein Cows Treated with Bovine Somatotropin;

The Economic Impacts of Various Public Policy Scenarios for Methane Recovery on Dairy Farms;

Dairy Research Herd Report;

Modeling Genetic and Environmental Effects of Test Day Records by Autoregressive Convariance Structures;

Effect of Human Chorionic Gonadotropin on Reproductive Performance of Lactating Dairy Cows;

A Corn Hybrid with High Cell Wall Content and Digestibility and Lactational Performance of Holstein Cows;

Comparison of Brown Midrib-6 and 18 Forage Sorghum with Conventional …

The Influence Of Diet On Faecal Dna Amplification And Sex Identification In Brown Bears (Ursus Arctos), Melanie A. Murphy, Lisette P. Waits, Katherine C. Kendall

United States National Park Service: Publications

To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears (Ursus arctos) were placed on six restricted diets: grass (Trifolium spp., Haplopappus hirtus and Poa pratensis), alfalfa (Lupinus spp.), carrots (Daucus spp.), white-tailed deer (Odocoileus virginianus ), blueberries (Vaccinium spp.) and salmon (Salmo spp.). DNA was extracted from 50 faecal samples of each restricted diet, and amplification of brown bear DNA was attempted for a mitochondrial DNA (mtDNA) locus and nuclear DNA (nDNA) locus. For mtDNA, no significant differences were observed in amplification success rates across diets. …

Grassland Bird Monitoring At Agate Fossil Beds National Monument, Nebraska And Tallgrass Prairie National Preserve, Kansas, David G. Peitz, Gareth A. Rowell

United States National Park Service: Publications

During the breeding seasons of 2001 through 2003, a total of 517 variable circular plots were surveyed for birds and assessed for habitat composition at Agate Fossil Beds National Monument, Nebraska and Tallgrass Prairie National Preserve, Kansas. Systematic 400 x 400 meter grids, originating from random start points, were used to locate 40 permanent variable circular plots in upland habitat at Agate Fossil Beds National Monument and 242 at Tallgrass Prairie National Preserve. An additional 14 permanent plots were established in the less common but important riparian habitat at Agate Fossil Beds National Monument, and 18 plots were added in …

Weathering Of Sulfidic Shale And Copper Mine Waste: Secondary Minerals And Metal Cycling In Great Smoky Mountains National Park, Tennessee, And North Carolina, Usa, Jane M. Hammarstrom, Robert R. Seal Ii, Allen L. Meier, John C. Jackson

United States Geological Survey: Staff Publications

Metal cycling via physical and chemical weathering of discrete sources (copper mines) and regional (non-point) sources (sulfide-rich shale) is evaluated by examining the mineralogy and chemistry of weathering products in Great Smoky Mountains National Park, Tennessee, and North Carolina, USA. The elements in copper mine waste, secondary minerals, stream sediments, and waters that are most likely to have negative impacts on aquatic ecosystems are aluminum, copper, zinc, and arsenic because these elements locally exceed toxicity guidelines for surface waters or for stream sediments. Acid-mine drainage has not developed in streams draining inactive copper mines. Acid-rock drainage and chemical weathering processes …

Supplementation To Meet Metabolizable Protein Requirements Of Primiparous Beef Heifers: I. Performance, Forage Intake, And Nutrient Balance, H. H. Patterson, Terry J. Klopfenstein, Don C. Adams, Jacqueline Musgrave

Department of Animal Science: Faculty Publications

Three experiments were conducted to evaluate the response of supplementing primiparous heifers based on the metabolizable protein (MP) system during pregnancy and lactation. In Exp. 1, 12 pregnant, March-calving heifers (432 ± 10 kg) grazing Sandhills range were randomly allotted to one of two treatments: supplementation based on either the MP system (MPR) or the CP system (CPR). Supplements were fed to individual heifers from October to February and no hay was offered. Grazed forage organic matter intake (FOMI) was measured in November, January, and February. In Exp. 2, 18 heifers (424 ± 8 kg) were randomly allotted to one …

Modification Of The Penn State Forage And Total Mixed Ration Particle Separator And The Effects Of Moisture Content On Its Measurements, P. J. Kononoff, A. J. Heinrichs, D. R. Buckmaster

Department of Animal Science: Faculty Publications

The Penn State Particle Separator has led to widespread measurement of forage and total mixed ration (TMR) particle size. However, a large proportion of small particles may pass through both sieves when a TMR is analyzed, and field research has suggested that both shaking frequency and sample dry matter may affect the results. The objectives of this project were to test the effects of an additional sieve with a smaller aperture size, shaking frequency, and sample moisture content on results obtained. A sieve was constructed out of wire with a nominal size aperture of 1.18 mm. Samples of alfalfa haylage, …

Estrogen Receptor-Α Populations Change With Age In Commercial Laying Hens, K.K. Hansen, R.J. Kittok, G. Sarath, C.F. Toombs, N. Caceres, M.M. Beck

Department of Animal Science: Faculty Publications

Older hens in production lay larger but fewer eggs than younger birds, and the incidence of soft and broken shells is greater in older hens than younger. These changes are attributable at least in part to changing hormone profiles and diminished ability of the hen to transport calcium at the duodenum. In further exploration of this relationship, a study was conducted with three ages of Hy-Line W-36 birds: prelay pullets (PL; 19 wk, 0% production), peak-production hens (PP; 29 wk, ∼93% production), and late-stage hens (LS; 71 wk, ∼80% production). Hens from the PP and LS groups were palpated for …

Isovolumetric Regulation In Mammal Cells: Role Of Taurine, B. Ordaz, R. Franco, National University Of Mexico Tuz

School of Veterinary and Biomedical Sciences: Faculty Publications

The ability to regulate cell volume is an ancient conserved trait present in essentially all species through evolution. The maintenance of a constant cell volume is a homeostatic imperative in animal cells. Changes in cell water content affecting the concentration of intracellular messenger molecules impair the complex signaling network, crucial for cell functioning and intercellular communication. Although the renal homeostatic mechanisms exert a precise control of extracellular fluid osmolarity, this is challenged in a variety of pathological situations. The intracellular volume constancy is continuously compromised by the generation of local and transient osmotic microgradients, associated with nutrients uptake, secretion, cytoskeleton …

Are Chlorella Viruses A Rich Source Of Ion Channel Genes?, Ming Kang, Anna Moroni, Sabrina Gazzarrini, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Plaque-forming dsDNA (>330 kb) viruses that infect certain unicellular, eukaryotic chlorella-like green algae contain ~375 protein-encoding genes. These proteins include a 94 amino acid K+ channel protein, called Kcv, as well as two putative ligand-gated ion channels. The viruses also encode other proteins that could be involved in the assembly and/ or function of ion channels, including protein kinases and a phosphatase, polyamine biosynthetic enzymes and histamine decarboxylase.

Fungal Biology And Agriculture: Revisiting The Field, O. Yarden, D. J. Ebbole, S. Freeman, R. J. Rodriguez, M. B. Dickman

Department of Plant Pathology: Faculty Publications

Plant pathology has made significant progress over the years, a process that involved overcoming a variety of conceptual and technological hurdles. Descriptive mycology and the advent of chemical plant-disease management have been followed by biochemical and physiological studies of fungi and their hosts. The later establishment of biochemical genetics along with the introduction of DNA-mediated transformation have set the stage for dissection of gene function and advances in our understanding of fungal cell biology and plant–fungus interactions. Currently, with the advent of high-throughput technologies, we have the capacity to acquire vast data sets that have direct relevance to the numerous …