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Una Nueva Especie De Alurnus Fabricius De La Costa Pacífica De Colombia Y Apuntes Sobre Su Biología (Coleoptera: Chrysomelidae: Cassidinae), Luis Carlos Pardo-Locarno, Luis Miguel Constantino Feb 2023

Una Nueva Especie De Alurnus Fabricius De La Costa Pacífica De Colombia Y Apuntes Sobre Su Biología (Coleoptera: Chrysomelidae: Cassidinae), Luis Carlos Pardo-Locarno, Luis Miguel Constantino

Insecta Mundi

Resumen. En este trabajo se describe una nueva especie de Alurnus Fabricius (Coleoptera: Chrysomelidae), A. chocoensis Pardo y Constantino, con notable coloración rojo sangre en todo el cuerpo, excepto las hembras que presentan el pronoto de color negro. La nueva especie es una plaga importante del follaje en palma de chontaduro (Bactris gasipaes Kunth) en la costa pacífica de Colombia y es un endemismo propio de las selvas lluviosas de la región biogeografica del Chocó, en el occidente de Colombia. Se presentan apuntes sobre su biología y ecología.

Abstract. In this work, a new species of Alurnus Fabricius (Coleoptera: …


Third Contribution To The Knowledge Of Arabian Onthophagini (Coleoptera: Scarabaeidae). Onthophagus Aeneopiceus D’Orbigny, 1902, A New Record For Yemen And For The Palaearctic Ecozone, Stefano Ziani, Denis Keith Feb 2023

Third Contribution To The Knowledge Of Arabian Onthophagini (Coleoptera: Scarabaeidae). Onthophagus Aeneopiceus D’Orbigny, 1902, A New Record For Yemen And For The Palaearctic Ecozone, Stefano Ziani, Denis Keith

Insecta Mundi

Onthophagus aeneopiceus d’Orbigny, 1902 (Coleoptera: Scarabaeidae: Scarabaeinae: Onthophag­ini) is recorded for the first time for Yemen and for the Palaearctic region. An integration into the key to the Onthophagini from the Arabian Peninsula (Ziani et al. 2019), is provided.

After Ziani et al. (2019) and Montanaro and Ziani (2022), 30 Onthophagini species were known from the Arabian Peninsula. In this paper a 31st species is recorded: Onthophagus aeneopiceus d’Orbigny, 1902. The species, belong­ing to the 23rd group of d’Orbigny (1913) and until now known from Eastern Africa and, dubitatively, Ivory Coast (Schoolmeesters 2022), has been collected in Yemen. This is …


Max And The Listening Tree, Paula Ray, Danny Reneau Feb 2023

Max And The Listening Tree, Paula Ray, Danny Reneau

Zea E-Books Collection

Max is a young man who lives with his grandmother. He is unhappy and upset, so he goes for a walk. His dad is in the army and his mom is a busy nurse. He is at a new school where kids make fun of his name. He wants to fly back to his old home and school. He wishes for someone to talk to. He sits down in the woods and his feelings come pouring out. Suddenly he hears from an old tree about deep roots and things always coming back. It’s his own tree for listening and for …


Endogenous L- To D-Amino Acid Residue Isomerization Modulates Selectivity Between Distinct Neuropeptide Receptor Family Members, Baba M. Yussif, Cole V. Blasing, James W. Checco Feb 2023

Endogenous L- To D-Amino Acid Residue Isomerization Modulates Selectivity Between Distinct Neuropeptide Receptor Family Members, Baba M. Yussif, Cole V. Blasing, James W. Checco

Department of Biochemistry: Faculty Publications

The L- to D-amino acid residue isomerization of neuropeptides is an understudied post-translational modification found in animals across several phyla. Despite its physiological importance, little information is available regarding the impact of endogenous peptide isomerization on receptor recognition and activation. As a result, the full roles peptide isomerization play in biology are not well understood. Here, we identify that the Aplysia allatotropin-related peptide (ATRP) signaling system utilizes L- to D-residue isomerization of one amino acid residue in the neuropeptide ligand to modulate selectivity between two distinct G protein-coupled receptors (GPCRs). We first identified a novel receptor for ATRP that is …


Bats Increased Foraging Activity At Experimental Prey Patches Near Hibernacula, Winifred F. Frick, Yvonne A. Dzal, Kristin A. Jonasson, Michael D. Whitby, Amanda M. Adams, Christen Long, John E. Depue, Christian M. Newman, Craig K. R. Willis, Tina L. Cheng Feb 2023

Bats Increased Foraging Activity At Experimental Prey Patches Near Hibernacula, Winifred F. Frick, Yvonne A. Dzal, Kristin A. Jonasson, Michael D. Whitby, Amanda M. Adams, Christen Long, John E. Depue, Christian M. Newman, Craig K. R. Willis, Tina L. Cheng

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

  1. Emerging infectious diseases in wildlife can threaten vulnerable host populations. Actions targeting habitat improvements to aid population resilience and recovery may be beneficial long-term strategies, yet testing the efficacy of such strategies before major conservation investments are made can be challenging.

  2. The disease white-nose syndrome (WNS) has caused severe declines in several species of North American hibernating bats. We tested a novel conservation approach targeted at improving foraging conditions near bat hibernacula by experimentally manipulating insect density in the pre-hibernation fattening period and spring emergence recovery period. We measured foraging (feeding buzzes) and echolocation activity of little brown bats Myotis …


Intraguild Predation Is Increased In Areas Of Low Prey Diversity In A Generalist Predator Community, Stella F. Uiterwaal, Amber J. Squires, Bennett A. Grappone, Brian Dillard, Ariadne Castaneda, Sora L. Kim, John P. Delong Feb 2023

Intraguild Predation Is Increased In Areas Of Low Prey Diversity In A Generalist Predator Community, Stella F. Uiterwaal, Amber J. Squires, Bennett A. Grappone, Brian Dillard, Ariadne Castaneda, Sora L. Kim, John P. Delong

School of Biological Sciences: Faculty Publications

  1. Niche differentiation and intraguild predation (IGP) can allow ecologically similar species to coexist, although it is unclear which coexistence mechanism predominates in consumer communities. Until now, a limited ability to quantify diets from metabarcoding data has precluded the use of sequencing data to determine the relative importance of these mechanisms.

  2. Here, we pair a recent metabarcoding quantification approach with stable isotope analysis to examine diet composition in a wolf spider community.

  3. We compare the prevalence of resource partitioning and IGP in these spiders and test whether factors that influence foraging performance, including individual identity, morphology, prey community and environmental conditions, …


The Waxy Mutation In Sorghum And Other Cereal Grains Reshapes The Gut Microbiome By Reducing Levels Of Multiple Beneficial Species, Qinnan Yang, Mallory J. Suhr Van Haute, Scott E. Sattler, Devin J. Rose, Anthony Juritsch, Jing Shao, Kristin A. Beede, Robert J. Schmaltz, Jeff Price, John Toy, Amanda E. Ramer-Tait, Andrew K. Benson Feb 2023

The Waxy Mutation In Sorghum And Other Cereal Grains Reshapes The Gut Microbiome By Reducing Levels Of Multiple Beneficial Species, Qinnan Yang, Mallory J. Suhr Van Haute, Scott E. Sattler, Devin J. Rose, Anthony Juritsch, Jing Shao, Kristin A. Beede, Robert J. Schmaltz, Jeff Price, John Toy, Amanda E. Ramer-Tait, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Waxy starches from cereal grains contain >90% amylopectin due to naturally occurring mutations that block amylose biosynthesis. Waxy starches have unique organoleptic characteristics (e.g. sticky rice) as well as desirable physicochemical properties for food processing. Using isogenic pairs of wild type sorghum lines and their waxy derivatives, we studied the effects of waxy starches in the whole grain context on the human gut microbiome. In vitro fermentations with human stool microbiomes show that beneficial taxonomic and metabolic signatures driven by grain from wild type parental lines are lost in fermentations of grain from the waxy derivatives and the beneficial signatures …


Sexual Selection As A Tool To Improve Student Reasoning Of Evolution, Sarah K. Spier, Joseph Dauer Feb 2023

Sexual Selection As A Tool To Improve Student Reasoning Of Evolution, Sarah K. Spier, Joseph Dauer

School of Natural Resources: Faculty Publications

There is an emphasis on survival-based selection in biology education that can allow students to neglect other important evolutionary components, such as sexual selection, reproduction, and inheritance. Student understanding of the role of reproduction in evolution is as important as student understanding of the role of survival. Limiting instruction to survival- based scenarios (e.g., effect of food on Galapagos finch beak shape) may not provide students with enough context to guide them to complete evolutionary reasoning. Different selection forces can work in concert or oppose one another, and sexual selection can lead to the selection of trait variants that are …


Leaf, Plant, To Canopy: A Mechanistic Study On Aboveground Plasticity And Plant Density Within A Maize–Soybean Intercrop System For The Midwest, Usa, Elena A. Pelech, Jochem B. Evers, Taylor L. Pederson, David W. Drag, Peng Fu, Carl J. Bernacchi Feb 2023

Leaf, Plant, To Canopy: A Mechanistic Study On Aboveground Plasticity And Plant Density Within A Maize–Soybean Intercrop System For The Midwest, Usa, Elena A. Pelech, Jochem B. Evers, Taylor L. Pederson, David W. Drag, Peng Fu, Carl J. Bernacchi

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plants have evolved to adapt to their neighbours through plastic trait responses. In intercrop systems, plant growth occurs at different spatial and temporal dimensions, creating a competitive light environment where aboveground plasticity may support complementarity in light-use efficiency, realizing yield gains per unit area compared with monoculture systems. Physiological and architectural plasticity including the consequences for light-use efficiency and yield in a maize-soybean solar corridor intercrop system was compared, empirically, with the standard monoculture systems of the Midwest, USA. The impact of reducing maize plant density on yield was investigated in the following year. Intercropped maize favoured physiological plasticity over …


Cover Crop Planting Practices Determine Their Performance In The U.S. Corn Belt, Katja Koehler-Cole, Roger W. Elmore, Humberto Blanco-Canqui, Charles A. Francis, Charles A. Shapiro, Christopher A. Proctor, Sabrina J. Ruis, Suat Irmak, Derek M. Heeren Feb 2023

Cover Crop Planting Practices Determine Their Performance In The U.S. Corn Belt, Katja Koehler-Cole, Roger W. Elmore, Humberto Blanco-Canqui, Charles A. Francis, Charles A. Shapiro, Christopher A. Proctor, Sabrina J. Ruis, Suat Irmak, Derek M. Heeren

Nebraska Cooperative Extension: Faculty and Staff Publications

Cover crop growing periods in the western United States Corn Belt could be extended by planting earlier. We evaluated both pre-harvest broadcast interseeding and post-harvest drilling of the following cover crops: (a) cereal rye (Secale cereale L.) [RYE]; (b) a mix of rye + legumes + brassicas [MIX1], (c) a mix of rye + oat [Avena sativa L.] + legumes + brassicas (MIX2), (d) legumes [LEGU]) and (e) a no cover crop control. These were tested in continuous corn (Zea mays L.) [corn–corn] and soybean [Glycine max (L.) Merr.]–corn systems [soybean–corn] at three sites in Nebraska …


Optimal Stomatal Theory Predicts Co2 Responses Of Stomatal Conductance In Both Gymnosperm And Angiosperm Trees, Anna Gardner, Mingkai Jiang, David S. Ellsworth, A. Robert Mackenzie, Jeremy Pritchard, Martin Karl Friedrich Bader, Craig V.M. Barton, Carl Bernacchi, Carlo Calfapietra, Kristine Y. Crous, Mirindi Eric Dusenge, Teresa E. Gimeno, Marianne Hall, Shubhangi Lamba, Sebastian Leuzinger, Johan Uddling, Jeffrey Warren, Göran Wallin, Belinda E. Medlyn Feb 2023

Optimal Stomatal Theory Predicts Co2 Responses Of Stomatal Conductance In Both Gymnosperm And Angiosperm Trees, Anna Gardner, Mingkai Jiang, David S. Ellsworth, A. Robert Mackenzie, Jeremy Pritchard, Martin Karl Friedrich Bader, Craig V.M. Barton, Carl Bernacchi, Carlo Calfapietra, Kristine Y. Crous, Mirindi Eric Dusenge, Teresa E. Gimeno, Marianne Hall, Shubhangi Lamba, Sebastian Leuzinger, Johan Uddling, Jeffrey Warren, Göran Wallin, Belinda E. Medlyn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Optimal stomatal theory predicts that stomata operate to maximise photosynthesis (Anet) and minimise transpirational water loss to achieve optimal intrinsic water-use efficiency (iWUE). We tested whether this theory can predict stomatal responses to elevated atmospheric CO2 (eCO2), and whether it can capture differences in responsiveness among woody plant functional types (PFTs). We conducted a meta-analysis of tree studies of the effect of eCO2 on iWUE and its components Anet and stomatal conductance (gs). We compared three PFTs, using the unified stomatal optimisation (USO) model to account for confounding effects of leaf–air vapour pressure difference (D). We expected smaller gs, but …


Invasive Annual Grasses—Reenvisioning Approaches In A Changing Climate, David Archer, David Toledo, Dana M. Blumenthal, Justin Derner, Usda Ars Burns, Oregon, Kirk Davies, Erik Hamerlynck, Roger Sheley, Pat Clark, Fred Pierson, Charlie Clements, Beth Newingham, Brian Rector, John Gaskin, Carissa L. Wonkka, Kevin Jensen, Tom Monaco, Lance T. Vermeire, Stephen L. Young Feb 2023

Invasive Annual Grasses—Reenvisioning Approaches In A Changing Climate, David Archer, David Toledo, Dana M. Blumenthal, Justin Derner, Usda Ars Burns, Oregon, Kirk Davies, Erik Hamerlynck, Roger Sheley, Pat Clark, Fred Pierson, Charlie Clements, Beth Newingham, Brian Rector, John Gaskin, Carissa L. Wonkka, Kevin Jensen, Tom Monaco, Lance T. Vermeire, Stephen L. Young

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

For nearly a century, invasive annual grasses have increasingly impacted terrestrial ecosystems across the western United States. Weather variability associated with climate change and increased atmospheric carbon dioxide (CO2) are making even more difficult the challenges of managing invasive annual grasses. As part of a special issue on climate change impacts on soil and water conservation, the topic of invasive annual grasses is being addressed by scientists at the USDA Agricultural Research Service to emphasize the need for additional research and future studies that build on current knowledge and account for (extreme) changes in abiotic and biotic conditions. Much research …


Blanding’S Turtle (Emydoidea Blandingii): Species Conservation Assessment, Melissa J. Panella, Colleen Rothe-Groleau Feb 2023

Blanding’S Turtle (Emydoidea Blandingii): Species Conservation Assessment, Melissa J. Panella, Colleen Rothe-Groleau

Nebraska Game and Parks Commission: Publications

The primary goal in development of at-risk species conservation assessments is to compile biological and ecological information that may assist conservation practitioners in making decisions regarding the conservation of species of interest. The Nebraska Natural Legacy Project recognizes the Blanding’s turtle (Emydoidea blandingii) as a Tier 1 at-risk species. Some general management recommendations are made here regarding Blanding’s turtles; however, conservation practitioners will need to use professional judgment to make specific management decisions based on objectives, location, and a multitude of variables. This resource was designed to share available knowledge of this at-risk turtle that will aid in the decision-making …


Arxiv Annual Report 2022, Cornell Tech Feb 2023

Arxiv Annual Report 2022, Cornell Tech

Copyright, Fair Use, Scholarly Communication, etc.

arXiv is a curated research-sharing platform open to anyone. As a pioneer in open access, arXiv.org now hosts more than 2.1 million scholarly articles in eight subject areas, curated by our strong community of volunteer moderators who balance content quality with distribution speed. arXiv provides an article submission portal, a TeX compilation service, search and discovery tools, web distribution for human readers,API access, machine readable data sets, and community-developed tools. Our emphasis on openness, collaboration, and scholarship provides the strong foundation on which arXiv thrives.

Mission — arXiv is an open platform where researchers can share and discover new, relevant, …


‘Chinese Virus’: A Critical Discourse Analysis Of Anti-Asian Racist Discourse During The Covid-19 Pandemic, Peiwen Wang, Theresa Catalano Feb 2023

‘Chinese Virus’: A Critical Discourse Analysis Of Anti-Asian Racist Discourse During The Covid-19 Pandemic, Peiwen Wang, Theresa Catalano

Department of Teaching, Learning, and Teacher Education: Faculty Publications

Since the emergence of COVID-19, researchers have documented an increase in cases of anti-Asian racism and hate crimes. Research shows a possible connection between the ‘Chinese virus’ discourse of the Trump administration and violence in society (Arora and Kim 2020:387). Drawing from critical discourse studies we explore 2,071 comments from one YouTube video which documents anti-China rhetoric by the Trump administration in order to understand the underlying strategies commenters relied on in their reproduction and defence of this discourse. Findings show the trickle-down influence of Trump’s discourse on YouTube commenters, but also ways in which social media created a platform …


An Evaluation Of Avian Influenza Virus Whole-Genome Sequencing Approaches Using Nanopore Technology, Hon S. Ip, Sarah Uhm, Mary Lea Killian, Mia K. Torchetti Feb 2023

An Evaluation Of Avian Influenza Virus Whole-Genome Sequencing Approaches Using Nanopore Technology, Hon S. Ip, Sarah Uhm, Mary Lea Killian, Mia K. Torchetti

United States Department of Agriculture Wildlife Services: Staff Publications

As exemplified by the global response to the SARS-CoV-2 pandemic, whole-genome sequencing played an important role in monitoring the evolution of novel viral variants and provided guidance on potential antiviral treatments. The recent rapid and extensive introduction and spread of highly pathogenic avian influenza virus in Europe, North America, and elsewhere raises the need for similarly rapid sequencing to aid in appropriate response and mitigation activities. To facilitate this objective, we investigate a next-generation sequencing platform that uses a portable nanopore sequencing device to generate and present data in real time. This platform offers the potential to extend in-house sequencing …


Development Of A Porous Layer-By-Layer Microsphere With Branched Aliphatic Hydrocarbon Porogens, Farah Shahjin, Milankumar Patel, Mahmudul Hasan, Jacob D. Cohen, Farhana Islam, Md Ashaduzzaman, Mohammad Ullah Nayan, Mahadevan Subramaniam, You Zhou, Irene Andreu, Howard E. Gendelman, Bhavesh D. Kevadiya Feb 2023

Development Of A Porous Layer-By-Layer Microsphere With Branched Aliphatic Hydrocarbon Porogens, Farah Shahjin, Milankumar Patel, Mahmudul Hasan, Jacob D. Cohen, Farhana Islam, Md Ashaduzzaman, Mohammad Ullah Nayan, Mahadevan Subramaniam, You Zhou, Irene Andreu, Howard E. Gendelman, Bhavesh D. Kevadiya

Nebraska Center for Biotechnology: Faculty and Staff Publications

Porous polymer microspheres are employed in biotherapeutics, tissue engineering, and regenerative medicine. Porosity dictates cargo carriage and release that are aligned with the polymer physicochemical properties. These include material tuning, biodegradation, and cargo encapsulation. How uniformity of pore size affects therapeutic delivery remains an area of active investigation. Herein, we characterize six branched aliphatic hydrocarbon-based porogen(s) produced to create pores in single and multilayered microspheres. The porogens are composed of biocompatible polycaprolactone, poly(lactic-co-glycolic acid), and polylactic acid polymers within porous multilayered microspheres. These serve as controlled effective drug and vaccine delivery platforms.


Fog Of War: How The Ukraine Conflict Transformed The Cyber Threat Landscape, Threat Analysis Group (Tag), Mandiant, Google Trust & Safety Feb 2023

Fog Of War: How The Ukraine Conflict Transformed The Cyber Threat Landscape, Threat Analysis Group (Tag), Mandiant, Google Trust & Safety

Copyright, Fair Use, Scholarly Communication, etc.

One year ago, Russia invaded Ukraine. Since then, tens of thousands of people have been killed, millions of Ukrainians have fled and the country has sustained tens of billions of dollars worth of damage. Importantly, this marks the first time that cyber operations have played such a prominent role in a world conflict.

Since the war began, governments, companies, civil society groups, and countless others have been working around the clock to support the Ukrainian people and their institutions. At Google, we support these efforts and continue to announce new commitments and support to Ukraine. This includes a donation of …


Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Mohamed, Asmaa A. M. Ahmed, Matías Schierenbeck, Mohamed Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam Jan 2023

Screening Spring Wheat Genotypes For Tadreb-B1 And Fehw3 Genes Under Severe Drought Stress At The Germination Stage Using Kasp Technology, Elsayed A. Mohamed, Asmaa A. M. Ahmed, Matías Schierenbeck, Mohamed Y. Hussein, P. Stephen Baenziger, Andreas Börner, Ahmed Sallam

Department of Agronomy and Horticulture: Faculty Publications

Drought stress is a major yield-limiting factor throughout the world in wheat (Triticum aestivum L.), causing losses of up to 80% of the total yield. The identification of factors affecting drought stress tolerance in the seedling stage is especially important to increase adaptation and accelerate the grain yield potential. In the current study, 41 spring wheat genotypes were tested for their tolerance to drought at the germination stage under two different polyethylene glycol concentrations (PEG) of 25% and 30%. For this purpose, twenty seedlings from each genotype were evaluated in triplicate with a randomized complete block design (RCBD) in …


Ultra-Fast Bioorthogonal Spin-Labeling And Distance Measurements In Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids, Subhashis Jana, Eric G. B. Evans, Hyo Sang Jang, Shuyang Zhang, Hui Zhang, Andrzej Rajca, Sharona E. Gordon, William N. Zagotta, Stefan Stoll, Ryan A. Mehl Jan 2023

Ultra-Fast Bioorthogonal Spin-Labeling And Distance Measurements In Mammalian Cells Using Small, Genetically Encoded Tetrazine Amino Acids, Subhashis Jana, Eric G. B. Evans, Hyo Sang Jang, Shuyang Zhang, Hui Zhang, Andrzej Rajca, Sharona E. Gordon, William N. Zagotta, Stefan Stoll, Ryan A. Mehl

Department of Chemistry: Faculty Publications

Studying protein structures and dynamics directly in the cellular environments in which they function is essential to fully understand the molecular mechanisms underlying cellular processes. Site-directed spin-labeling (SDSL)—in combination with double electron–electron resonance (DEER) spectroscopy—has emerged as a powerful technique for determining both the structural states and the conformational equilibria of biomacromolecules. In-cell DEER spectroscopy on proteins in mammalian cells has thus far not been possible due to the notable challenges of spin-labeling in live cells. In-cell SDSL requires exquisite biorthogonality, high labeling reaction rates and low background signal from unreacted residual spin label. While the bioorthogonal reaction must be …


Amicus Brief - Eggers V. Evnen, No. 22-2268, 8th Circuit, August 2, 2022, Anthony Schutz Jan 2023

Amicus Brief - Eggers V. Evnen, No. 22-2268, 8th Circuit, August 2, 2022, Anthony Schutz

Nebraska State Constitution

Because Nebraska’s constitutional provision for direct democracy involves voting and the exercise of the People’s legislative authority, it is subject to the principles of Reynolds v. Sims and the Fourteenth Amendment to the U.S. Constitution. This understanding of the People’s power of initiative and referendum as legislative is supported by Nebraska history, as is the severability of the two-fifths, county-distribution requirement from Article III, § 2 of the Nebraska Constitution. The county-distribution requirement fails the one-person-one-vote constraint placed upon States by the federal constitution. As a matter of statistical analysis, that requirement gives more power to voters in counties with …


Supplementation Of Sulfide Or Acetate And 2-Mercaptoethane Sulfonate Restores Growth Of The Methanosarcina Acetivorans Δhdrabc Deletion Mutant During Methylotrophic Methanogenesis, Alicia M. Salvi, Niaz Bahar Chowdhury, Rajib Saha, Nicole R. Buan Jan 2023

Supplementation Of Sulfide Or Acetate And 2-Mercaptoethane Sulfonate Restores Growth Of The Methanosarcina Acetivorans Δhdrabc Deletion Mutant During Methylotrophic Methanogenesis, Alicia M. Salvi, Niaz Bahar Chowdhury, Rajib Saha, Nicole R. Buan

Department of Biochemistry: Faculty Publications

Methanogenic archaea are important organisms in the global carbon cycle that grow by producing methane gas. Methanosarcina acetivorans is a methanogenic archaeum that can grow using methylated compounds, carbon monoxide, or acetate and produces renewable methane as a byproduct. However, there is limited knowledge of how combinations of substrates may affect metabolic fluxes in methanogens. Previous studies have shown that heterodisulfide reductase, the terminal oxidase in the electron transport system, is an essential enzyme in all methanogens. Deletion of genes encoding the nonessential methylotrophic heterodisulfide reductase enzyme (HdrABC) results in slower growth rate but increased metabolic efficiency. We hypothesized that …


Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu Jan 2023

Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu

Nebraska Center for Virology: Faculty Publications

Membrane proteins expressed on the surface of enveloped viruses are conformational antigens readily recognized by B cells of the immune system. An effective vaccine would require the synthesis and delivery of these native conformational antigens in lipid membranes that preserve specific epitope structures. We have created an extracellular vesicle–based technology that allows viral membrane antigens to be selectively recruited onto the surface of WW domain–activated extracellular vesicles (WAEVs). Budding of WAEVs requires secretory carrier-associated membrane protein 3, which through its proline-proline-alanine-tyrosine motif interacts with WW domains to recruit fused viral membrane antigens onto WAEVs. Immunization with influenza and HIV viral …


Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu Jan 2023

Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu

Nebraska Center for Virology: Faculty Publications

Membrane proteins expressed on the surface of enveloped viruses are conformational antigens readily recognized by B cells of the immune system. An effective vaccine would require the synthesis and delivery of these native conformational antigens in lipid membranes that preserve specific epitope structures. We have created an extracellular vesicle–based technology that allows viral membrane antigens to be selectively recruited onto the surface of WW domain–activated extracellular vesicles (WAEVs). Budding of WAEVs requires secretory carrier-associated membrane protein 3, which through its proline-proline-alanine-tyrosine motif interacts with WW domains to recruit fused viral membrane antigens onto WAEVs. Immunization with influenza and HIV viral …


Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu Jan 2023

Displaying And Delivering Viral Membrane Antigens Via Ww Domain–Activated Extracellular Vesicles, Sengjin Choi, Zhiping Yang, Qiyu Wang, Zhi Qiao, Maoyun Sun, Joshua Wiggins, Shi-Hua Xiang, Quan Lu

School of Veterinary and Biomedical Sciences: Faculty Publications

Membrane proteins expressed on the surface of enveloped viruses are conformational antigens readily recognized by B cells of the immune system. An effective vaccine would require the synthesis and delivery of these native conformational antigens in lipid membranes that preserve specific epitope structures. We have created an extracellular vesicle–based technology that allows viral membrane antigens to be selectively recruited onto the surface of WW domain–activated extracellular vesicles (WAEVs). Budding of WAEVs requires secretory carrier-associated membrane protein 3, which through its proline-proline-alanine-tyrosine motif interacts with WW domains to recruit fused viral membrane antigens onto WAEVs. Immunization with influenza and HIV viral …


Vaccines Against Group B Coxsackieviruses And Their Importance, Kiruthiga Mone, Ninaad Lasrado, Meghna Sur, Jay Reddy Jan 2023

Vaccines Against Group B Coxsackieviruses And Their Importance, Kiruthiga Mone, Ninaad Lasrado, Meghna Sur, Jay Reddy

School of Veterinary and Biomedical Sciences: Faculty Publications

The group B coxsackieviruses (CVBs) exist in six serotypes (CVB1 to CVB6). Disease associations have been reported for most serotypes, and multiple serotypes can cause similar diseases. For example, CVB1, CVB3, and CVB5 are generally implicated in the causation of myocarditis, whereas CVB1 and CVB4 could accelerate the development of type 1 diabetes (T1D). Yet, no vaccines against these viruses are currently available. In this review, we have analyzed the attributes of experimentally tested vaccines and discussed their merits and demerits or limitations, as well as their impact in preventing infections, most importantly myocarditis and T1D.


Diversity, Distribution, And Methodological Considerations Of Haemosporidian Infections Among Galliformes In Alaska, Faith De Amaral, Robert E. Wilson, Sarah A. Sonsthagen, Ravinder Sehgal Jan 2023

Diversity, Distribution, And Methodological Considerations Of Haemosporidian Infections Among Galliformes In Alaska, Faith De Amaral, Robert E. Wilson, Sarah A. Sonsthagen, Ravinder Sehgal

School of Natural Resources: Faculty Publications

Using samples spanning 10-degrees of latitude in Alaska, we provide the first comparative assessment of avian haemosporidia distribution of Arctic Alaska with subarctic host populations for four species of grouse and three species of ptarmigan (Galliformes). We found a high overall prevalence for at least one haemospordian genus (88%; N = 351/400), with spruce grouse (Canachites canadensis) showing the highest prevalence (100%; N = 54/ 54). Haemoproteus and Plasmodium lineages were only observed within grouse, while Leucocytozoon species were found within both grouse and ptarmigan. Further, different Leucocytozoon lineages were obtained from blood and tissue samples from the …


Evaluation Of Pollination Traits Important For Hybrid Wheat Development In Great Plains Germplasm, Nicholas Garst, Vikas Belamkar, Amanda Easterly, Mary J. Guttieri, Hannah Stoll, Amir M. H. Ibrahim, P. Stephen Baenziger Jan 2023

Evaluation Of Pollination Traits Important For Hybrid Wheat Development In Great Plains Germplasm, Nicholas Garst, Vikas Belamkar, Amanda Easterly, Mary J. Guttieri, Hannah Stoll, Amir M. H. Ibrahim, P. Stephen Baenziger

Department of Agronomy and Horticulture: Faculty Publications

Hybrid wheat (Triticum aestivumL.) offers potential yield advantages over conventional inbred cultivars. For hybrid wheat to be a commercial success, the cost to produce the hybrid seed needs to be minimized. Although wheat is naturally selfpollinated, hybrid wheat seed production can be improved by increasing the amount and availability of pollen for cross-pollination. This research examined 19 pollination traits using the Hard Winter Wheat Association Mapping Panel for 3 years. Anther extrusion, pollen 50 date (date at which a genotype has 50% of spikes pollinating), plant height, and pollination duration (last spike pollen 50 date minus first spike …


The Influence Of Conditions In Lake Superior And The Bois Brule River, Wisconsin On Returns Of Migratory Rainbow Trout, T.R. Hrabik, K.W. Olson, T.J. Kaspar, M.E. Sierszen, B.G. Matthias Jan 2023

The Influence Of Conditions In Lake Superior And The Bois Brule River, Wisconsin On Returns Of Migratory Rainbow Trout, T.R. Hrabik, K.W. Olson, T.J. Kaspar, M.E. Sierszen, B.G. Matthias

United States Fish and Wildlife Service: Publications

Rainbow trout were introduced to Lake Superior in the late 1800’s and exhibit a potamodromous life history and exhibit high variability in reproductive success. We examined reproductive variability in the Bois Brule River, WI (Lake Superior), through analyses of returns of wild first spawning (hereafter ‘‘maiden” returning) adults. We used classification and regression tree analyses to identify in-stream and in-lake (western Lake Superior) sources of variability and to identify the environment (stream or lake) that was most influential to the returns to each location. Among in-stream influences, high discharge rates in the spring period (March – May) during a pre-smolt’s …


Refined Teaching Methods, Systems Thinking, And Experiential Approaches Enhanced Students Learning Through Covid-19, Osler Ortez, Alyssa Kuhn, Meghan Sindelar Jan 2023

Refined Teaching Methods, Systems Thinking, And Experiential Approaches Enhanced Students Learning Through Covid-19, Osler Ortez, Alyssa Kuhn, Meghan Sindelar

Department of Agronomy and Horticulture: Faculty Publications

The Soil Nutrient Relationships course serves juniors and seniors with a major or minor in agronomy at the University of Nebraska-Lincoln. Pre-pandemic enrollment averaged 65 students. In 2021 and 2022, course enrollment was 42 and 55, respectively. The course was adjusted to a flipped design in 2017. Moving into 2021, the Soil Nutrient Relationships course underwent a major overhaul by changing the content source materials and organization of lab activities while maintaining the flipped delivery format. While responding to the COVID-19 pandemic limitations, the redesign was intended to focus limited face-to-face meetings (in person or webconference) on problem-solving activities. This …