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Nebline, October 2022

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

4-H Youth Thrive at Super Fair With Community Support

Apples by Nebraska Extension Staff and Interns

Recipes of the Month: Crunchy Apple Roll and Easy Apple Salad by Kayla Colgrove

Fall Watering Aids Tree & Shrub Health by Sarah Browning

Garden Guide: Things to Do This Month

Maintaining Your Septic System by Becky Schuerman

A Wasp in Disguise: The Velvet Ant by Kait Chapman

4-H’ers Test Family and Consumer Science Skills at Life Challenge

4-H’ers Test Their Knowledge of Animals and Decision-Making Skills at PASE

4-H Announcements for 4-H'ers and Volunteers

Heart of 4-H Volunteer Award: Capital City Horse and …

The Role Of Intertemporal Preferences, Active Consideration Of Health Outcomes, And Simple Health Prompts On The Nutritional Quality Of Food Choices, Olivier Tuyizere

Department of Agricultural Economics: Dissertations, Theses, and Student Research

This thesis consists of three chapters. The first chapter introduces the thesis by highlighting a brief review of intertemporal preferences, active consideration of health outcomes, and health prompts during food choices. The introduction paves the way for the following two chapters, which are related, but stand-alone papers.

In the second chapter, we explore a novel question: how does actively considering health outcomes (both current and future) during decision-making affect the nutritional quality of food choices? We explore this question with an online experiment on food choices. Our findings show that active consideration of health outcomes leads to choosing products with …

Effects Of Early‑Life Experience On Innovation And Problem‑Solving In Captive Coyotes, Andrew C. Garcia, Mitchell A. Parsons, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Early-life experience often shapes behaviors like innovation and exploration. These behaviors are important to animals encountering novel food resources in diverse habitats, such as mesocarnivores in urban areas. To understand if early-life experiences impact later-life behavior, we examined how coyotes (Canis latrans) responded to a multi-access puzzle box at two life stages: pup (~ 7 weeks) and dispersal (~ 10 months). We first exposed pups, still living with their parents and littermates, to a baited puzzle box. At dispersal age, we again tested both these pups and an age-matched control group that was not exposed to the puzzle …

Genetic Analysis Of Seed Traits In Sorghum Bicolor That Affect The Human Gut Microbiome, Quinnan Yang, Mallory J. Suhr Van Haute, Nate Korth, Scott E. Sattler, John Toy, James C. Schnable, Andrew K. Benson

Department of Food Science and Technology: Faculty Publications

Prebiotic fibers, polyphenols and other molecular components of food crops significantly affect the composition and function of the human gut microbiome and human health. The abundance of these, frequently uncharacterized, microbiome-active components vary within individual crop species. Here, we employ high throughput in vitro fermentations of pre-digested grain using a human microbiome to identify segregating genetic loci in a food crop, sorghum, that alter the composition and function of human gut microbes. Evaluating grain produced by 294 sorghum recombinant inbreds identifies 10 loci in the sorghum genome associated with variation in the abundance of microbial taxa and/or microbial metabolites. Two …

End-Use Quality Of Historical And Modern Winter Wheats Adapted To The Great Plains Of The United States, Sujun Liu, Lan Xu, Yifan Wu, Senay Simsek, Devin J. Rose

Department of Food Science and Technology: Faculty Publications

Improving milling and baking properties is important during wheat breeding. To determine changes in milling and baking quality of hard winter wheat, 23 adapted cultivars released in the Great Plains between 1870 and 2013 were grown in triplicate in a single location (Mead, NE, USA) over two crop years (2018 and 2019). Grain yield and kernel hardness index increased by release year (p < 0.05). The observed increase in hardness index was accompanied by a decrease in percent soft kernels (p < 0.05). Diameter and weight decreased with release year in 2019 (p < 0.05), and their standard deviation increased with the release year (p < 0.05). Flour protein content decreased with release year (p < 0.05) and dough mixing quality increased (p < 0.05). No significant relationship was found for baking property variables, but bran water retention capacity (BWRC), which is correlated with whole wheat bread quality, increased with release year (p < 0.05). In conclusion, wheat kernels have become harder but more variable in shape over a century of breeding. Mixing quality showed significant improvements, and loaf volume and firmness remained constant, even in the presence of a decrease in protein concentration. Bran quality decreased across release year, which may have implications for whole grain baking quality and milling productivity.

Setting The Stage For The Next Farm Bill Debate, Bradley D. Lubben

Cornhusker Economics

The 2018 Farm Bill is set to expire in September 2023. New farm bill legislation will be needed by then if authority is to be extended for a wide range of programs from farm support to conservation, nutrition assistance, credit, trade promotion, rural development, research and education, and more.

The agricultural committees in Congress have already held initial hearings and many agricultural and other interest groups have noted their policy priorities. However, formal debate on a new farm bill is not expected to begin in earnest until early 2023 when a new session of Congress convenes.

While the formal debate …

Multiple Generations Of Antibiotic Exposure And Isolation Influence Host Fitness And The Microbiome In A Model Zooplankton Species, Reilly O. Cooper, Sarah Tjards, Jessica Rischling, David T. Nguyen, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Background

Chronic antibiotic exposure impacts host health through changes to the microbiome, increasing disease risk and reducing the functional repertoire of community members. The detrimental effects of antibiotic perturbation on microbiome structure and function after one host generation of exposure have been well-studied. However, much less is understood about the multigenerational effects of antibiotic exposure and how the microbiome may recover across host generations.

Results

In this study, we examined microbiome composition and host fitness across five generations of exposure to a suite of three antibiotics in the model zooplankton host Daphnia magna. By utilizing a split-brood design where …

How Digital Is Agriculture In A Subset Of Countries From South America? Adoption And Limitations, Laila A. Puntel, É. L. Bolfe, R. J. M. Melchiori, R. Ortega, G. Tiscornia, A. Roel, F. Scaramuzza, S. Best, A. G. Berger, D. S. S. Hansel, D. Palacios Durán, G. R. Balboa

Department of Agronomy and Horticulture: Faculty Publications

Digital agriculture (DA) can contribute solutions to meet an increase in healthy, nutritious, and affordable food demands in an efficient and sustainable way. South America (SA) is one of the main grain and protein producers in the world but the status of DA in the region is unknown. A systematic review and case studies from Brazil, Argentina, Uruguay, and Chile were conducted to address the following objectives: (1) quantify adoption of existing DA technologies, (2) identify limitations for DA adoption; and (3) summarise existing metrics to benchmark DA benefits. Level of DA adoption was led by Brazil and Argentina followed …

Multi-Transcriptome Analysis Following An Acute Skeletal Muscle Growth Stimulus Yields Tools For Discerning Global And Myc Regulatory Networks, Kevin A. Murach, Zhengye Liu, Baptiste Jude, Vandre C. Figueiredo, Yuan Wen, Sabin Khadgi, Seongkyun Lim, Francielly Morena Da Silva, Nicholas P. Greene, Johanna T. Lanner, John J. Mccarthy, Ivan Jose Vechetti Jr, Ferdinand Von Walden

Department of Nutrition and Health Sciences: Faculty Publications

Myc is a powerful transcription factor implicated in epigenetic reprogramming, cellular plasticity, and rapid growth as well as tumorigenesis. Cancer in skeletal muscle is extremely rare despite marked and sustained Myc induction during loading-induced hypertrophy. Here, we investigated global, actively transcribed, stable, and myonucleus-specific transcriptomes following an acute hypertrophic stimulus in mouse plantaris. With these datasets, we define global and Myc-specific dynamics at the onset of mechanical overload-induced muscle fiber growth. Data collation across analyses reveals an under-appreciated role for the muscle fiber in extracellular matrix remodeling during adaptation, along with the contribution of mRNA stability to epigenetic-related transcript …

Different Gut Microbiomes Of Developmental Stages Of Field-Collected Native And Invasive Western Bean Cutworm, Striacosta Albicosta, In Western Nebraska, Paul A. Ayayee, Austin Currie, Julie A. Peterson

Department of Entomology: Faculty Publications

While insects harbor gut microbial associates that perform various functions for the host, lepidopterans have not been considered as prime examples of having such relationships. The western bean cutworm, Striacosta albicosta (Lepidoptera: Noctuidae), is native to North America and has historically been a significant corn pest in its western distribution. It is currently expanding eastwards and is invasive in these new regions. Using 16S rRNA gene sequencing data, this study focused on characterizing the microbiota associated with field-collected eggs, larvae, adults, and host plant materials of S. albicosta in its native range. The diversity of microbiomes varied significantly among S. …

Lessons Learned From The 2019 Nebraska Floods: Implications For Emergency Management, Mass Care, And Food Security, Eric E. Calloway, Nadine B. Nugent, Katie L. Stern, Ashley Mueller, Amy L. Yaroch

Nebraska Cooperative Extension: Faculty and Staff Publications

This qualitative study aimed to understand the actions, challenges, and lessons learned for addressing the food and water needs of flood survivors, with a special focus on vulnerable populations and the implications for food security, to inform future disaster response efforts in the U.S. Semi-structured in-depth interviews were conducted from January to August 2020 with the local, state, and national stakeholders (n = 27) involved in the disaster response to the 2019 Nebraska floods, particularly those involved in providing mass care, such as food, water, and shelter, for the flood survivors. The challenge themes were related to limited risk …

Evaluation Of The Effects Of Wood-Sourced Biochar As A Feedlot Pen Surface Amendment On Manure Nutrient Capture, Jessica L. Sperber, Galen E. Erickson, Andrea K. Watson

Department of Animal Science: Faculty Publications

Feedstuffs utilized in U.S. feedlot finishing rations incorporate high concentrations of N and P, with less than 15% of fed N and P retained by the animal. The remaining N and P are excreted in the manure, where the opportunity for manure N loss via ammonia (NH3) volatilization from the feedlot pen surface is a risk to the environment and lowers the value of manure as a fertilizer. Two nutrient mass balance experiments were conducted during the winter and summer seasons to evaluate the effects of spreading unprocessed Eastern red cedar biochar onto the feedlot pen surface on manure nutrient …

Can Body Mass Index Influence The Skin Temperature Of Adolescents? A Preliminary Study With The Use Of Infrared Thermography, Hamilton Henrique Teixeira Reis, Ciro José Brito, Alisson Gomes Da Silva, Manuel Sillero-Quintana, Victória Esther Teixeria Reis, Francisco Zacaron Werneck, Ismael Fernández-Cuevas, Matheus Santos Cerqueira, João Carlos Bouzas Marins

Department of Nutrition and Health Sciences: Faculty Publications

– Infrared thermography (IRT) has been used to assess skin temperature (Tsk), especially during the COVID-19 pandemic, as an important tool in medical screening not only of the general population, but also of young athletes. However, the subcutaneous adipose tissue can act as an insulator when the Tsk is assessed by IRT, modifying the normal Tsk data and leading to their misinterpretation. Considering that the body mass index (BMI) is an important predictor of obesity, the objective of this study was to verify if the Tsk measured by IRT is affected by the BMI in adolescents. A preliminary study was …

Conversations About Food Insecurity: Examining College Campus Climates, Crystal Eufemia Garcia

Department of Educational Administration: Faculty Publications

This qualitative critical narrative inquiry study explored the experiences of 19 students within four universities in a single Southern state that used campus food aid resources such as a food pantry. Using Hurtado et al.’s Multicontextual Model for Diverse Learning Environments (MMDLE), this paper sheds light on how participants discussed the campus climate for students experiencing food insecurity within their respective campuses. Findings unpack students’ reflections on a lack of awareness and discussion about food insecurity and food aid resources within the campus community, the role that stigmas played in participants’ perceptions and use of campus food aid resources, and …

Prebiotics Enhance Persistence Of Fermented-Food Associated Bacteria In In Vitro Cultivated Fecal Microbial Communities, Chloe M. Christensen, Car Reen Kok, Jennifer Auchtung, Robert Hutkins

Department of Food Science and Technology: Faculty Publications

It is well established that the gastrointestinal (GI) microbiota plays a major role in human health. Dietary interventions, and consumption of fermented foods that contain live microbes, in particular, are among the approaches being investigated to modulate the GI microbiota and improve health. However, the persistence of fermented food-associated bacteria (FAB) within the GI tract is typically limited by host factors that limit colonization and competition with autochthonous microbes. In this research, we examined if the addition of prebiotics, dietary substrates that are selectively metabolized by microbes to improve health, would enhance the persistence of FAB. We evaluated the persistence …

Patch-Burn Grazing Impacts Forage Resources In Subtropical Humid Grazing Lands, Elizabeth H. Boughton, Nuria Gomez-Casanovas, Hilary Swain, Carl Bernacchi, Raoul K. Boughton, Keith Brinsko, Haoyu Li, Alan Rivero, Evan H. Delucia, Jed Sparks

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Subtropical humid grazing lands represent a large global land use and are important for livestock production, as well as supplying multiple ecosystem services. Patch-burn grazing (PBG) management is applied in temperate grazing lands to enhance environmental and economic sustainability; however, this management system has not been widely tested in subtropical humid grazing lands. The objective of this study was to determine how PBG affected forage resources, in comparison with the business-as-usual full-burn (FB) management in both intensively managed pastures (IMP) and seminative (SN) pastures in subtropical humid grazinglands. We hypothesized that PBG management would create patch contrasts in forage quantity …

Host Species And Captivity Distinguish The Microbiome Compositions Of A Diverse Zoo-Resident Non-Human Primate Population, Maya O. Wills, Robin R. Shields-Cutler, Emily Brunmeier, Madison Weissenborn, Tami Murphy, Dan Knights, Timothy J. Johnson, Jonathan B. Clayton

Department of Food Science and Technology: Faculty Publications

Vast numbers of microorganisms inhabit the mammalian gastrointestinal tract in a complex community referred to as the gut microbiome. An individual’s microbiome may be impacted by genetics, diet, and various environmental factors, and has been associated with many health states and diseases, though specific explanations are lacking. While these communities are well-studied in human populations, non-human primates (NHPs), in particular zoo-resident or captive NHPs, offer distinct advantages to increasing our understanding of factors that influence gut microbiome composition. Here, we characterize the gut microbiome composition of a phylogenetically diverse cohort of NHPs residing in the same urban zoo. We show …

Scaling Cellular Agriculture Production With Photolithography: A Study On Curing And Degradation, Alexis Garrett

Department of Mechanical and Materials Engineering: Dissertations, Theses, and Student Research

To meet the requirements for full production in cellular agriculture, a multi-disciplinary approach is required. New questions and understandings between different concepts, such as texture and cooking, need to be identified and how they correlate with cellular behavior and taste. This work focuses on understanding and developing photolithographic systems for scalable cellular agriculture. There were two specific research directions: 1) a traditional photolithography printing system was improved upon for mass manufacturing purposes and 2) mechanical analysis and degradation of key potential photolithographic biomaterials were tested for a food product. A novel digital rotational ultraviolet manufacturing (DRUM) process was developed, and …

Biofilms: Formation, Research Models, Potential Targets, And Methods For Prevention And Treatment, Yajuan Su, Jaime T. Yrastorza, Mitchell Matis, Jenna Cusick, Siwei Zhao, Guangshun Wang, Jingwei Xie

Department of Mechanical and Materials Engineering: Faculty Publications

Due to the continuous rise in biofilm-related infections, biofilms seriously threaten human health. The formation of biofilms makes conventional antibiotics ineffective and dampens immune clearance. Therefore, it is important to understand the mechanisms of biofilm formation and develop novel strategies to treat biofilms more effectively. This review article begins with an introduction to biofilm formation in various clinical scenarios and their corresponding therapy. Established biofilm models used in research are then summarized. The potential targets which may assist in the development of new strategies for combating biofilms are further discussed. The novel technologies developed recently for the prevention and treatment …

Ncoa4 Regulates Iron Recycling And Responds To Hepcidin Activity And Lipopolysaccharide In Macrophages, Cole A. Guggisberg, Juyoung Kim, Jaekwon Lee, Xiaoli Chen, Moon-Suhn Ryu

Department of Biochemistry: Faculty Publications

Macrophages, via erythrophagocytosis, recycle iron from effete erythrocytes to newly developing red blood cells. Conversion of potentially cytotoxic levels of iron from its heme into nonheme form during iron recycling is safely accomplished via coordinated regulations of cellular iron transport and homeostasis. Herein, we demonstrate the roles and regulation of NCOA4 (nuclear receptor coactivator 4)-mediated ferritinophagy in macrophages after erythrophagocytosis using the mouse macrophage cell line J774 cells. Ferritin in J774 cells increased with the rise of nonheme iron by erythrocyte ingestion and declined when total cellular iron contents subsequently decreased. NCOA4, a selective autophagic cargo receptor for ferritin, was …

Girls In Stem! Elementary Afterschool Six-Week Curriculum, Jess Li, Samavi Rafique

Honors Program: Expanded Learning Clubs

No abstract provided.

Metabolic Model Of Necrotizing Enterocolitis In The Premature Newborn Gut Resulting From Enteric Dysbiosis, Giorgio Casaburi, Jingjing Wei, Sufyan Kazi, Junlin Liu, Kewei Wang, Guo-Zhong Tao, Po-Yu Lin, James C. Y. Dunn, Bethany M. Henrick, Steven A. Frese, Karl G. Sylvester

Department of Nutrition and Health Sciences: Faculty Publications

Necrotizing enterocolitis (NEC) is a leading cause of premature newborn morbidity and mortality. The clinical features of NEC consistently include prematurity, gut dysbiosis and enteral inflammation, yet the pathogenesis remains obscure. Herein we combine metagenomics and targeted metabolomics, with functional in vivo and in vitro assessment, to define a novel molecular mechanism of NEC. One thousand six hundred and forty seven publicly available metagenomics datasets were analyzed (NEC = 245; healthy = 1,402) using artificial intelligence methodologies. Targeted metabolomic profiling was used to quantify the concentration of specified fecal metabolites at NEC onset (n = 8), during recovery ( …

Differential Progression Of Unhealthy Dietinduced Hepatocellular Carcinoma In Obese And Non-Obese Mice, Emma Hymel, Elizabeth Vlock, Kurt W. Fisher, Paraskevi A. Farazi

Department of Nutrition and Health Sciences: Faculty Publications

Background Non-alcoholic fatty liver disease (NAFLD) ranks first among liver diseases in Western countries. NAFLD is typically associated with obesity and diabetes, however it also develops in lean individuals without metabolic syndrome. The prevalence of lean NAFLD is 7 percent in the U.S. and 25–30 percent in some Asian countries. NAFLD starts with excess liver fat accumulation (NAFL), progresses to nonalcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma (HCC). The pathogenesis of lean NASH-HCC and how it differs from obese NASH-HCC is not well understood.

Methods In this work, we generated a mouse model of lean and obese NASH-HCC using a …

Differential Progression Of Unhealthy Dietinduced Hepatocellular Carcinoma In Obese And Non-Obese Mice, Emma Hymel, Elizabeth M. Vlock, Kurt W. Fisher, Parashevi A. Farazi

Department of Food Science and Technology: Faculty Publications

Background

Non-alcoholic fatty liver disease (NAFLD) ranks first among liver diseases in Western countries. NAFLD is typically associated with obesity and diabetes, however it also develops in lean individuals without metabolic syndrome. The prevalence of lean NAFLD is 7 percent in the U.S. and 25–30 percent in some Asian countries. NAFLD starts with excess liver fat accumulation (NAFL), progresses to nonalcoholic steatohepatitis (NASH), cirrhosis and hepatocellular carcinoma (HCC). The pathogenesis of lean NASH-HCC and how it differs from obese NASH-HCC is not well understood.

Methods

In this work, we generated a mouse model of lean and obese NASH-HCC using a …

Effects Of Spatially Heterogeneous Lakeside Development On Nearshore Biotic Communities In A Large, Deep, Oligotrophic Lake, Michael F. Meyer, Ted Ozersky, Kara H. Woo, Kirill Shchapov, Aaron W. E. Galloway, Julie B. Schram, Emma J. Rosi, Daniel D. Snow, Maxim A. Timofeyev, Dmitry Yu. Karnaukhov, Matthew R. Brousil, Stephanie E. Hampton

School of Natural Resources: Faculty Publications

Sewage released from lakeside development can reshape ecological communities. Nearshore periphyton can rapidly assimilate sewage-associated nutrients, leading to increases of filamentous algal abundance, thus altering both food abundance and quality for grazers. In Lake Baikal, a large, ultra-oligotrophic, remote lake in Siberia, filamentous algal abundance has increased near lakeside developments, and localized sewage input is the suspected cause. These shifts are of particular interest in Lake Baikal, where endemic littoral biodiversity is high, lakeside settlements are mostly small, tourism is relatively high (~1.2 million visitors annually), and settlements are separated by large tracts of undisturbed shoreline, enabling investigation of heterogeneity …

Snp Discovery In Proso Millet (Panicum Miliaceum L.) Using Lowpass Genome Sequencing, Rituraj Khound, Guangchao Sun, Ravi V. Mural, James C. Schnable, Dipak K. Santra

Department of Agronomy and Horticulture: Faculty Publications

Domesticated ~10,000 years ago in northern China, Proso millet (Panicum miliaceum L.) is a climate-resilient and human health-promoting cereal crop. The genome size of this self-pollinated allotetraploid is 923 Mb. Proso millet seeds are an important part of the human diet in many countries. In the USA, its use is restricted to the birdseed and pet food market. Proso millet is witnessing gradual demand in the global human health and wellness food market owing to its health-promoting properties such as low glycemic index and gluten-free. The breeding efforts for developing improved proso millet cultivars are hindered by the …

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …

Bacterial Diseases Of Tilapia, Their Zoonotic Potential And Risk Of Antimicrobial Resistance, Olga L. M. Haenen, Ha Thanh Dong, Truong Dinh Hoai, Margaret Crumlish, Iddya Karunasagar, Timothy Barkham, Swaine L. Chen, Ruth Zadoks, Andreas Kiermeier, Bing Wang, Esther Garrido Gamarro, Masami Takeuchi, Mohammad Noor Amal Azma, Belén Fouz, Southeast Asian Fisheries Development Center, Zeng Wei Wei, Melba G. Bondad-Reantaso

School of Natural Resources: Faculty Publications

Tilapia culture is an important source of income and nutrition to many rural families. Since 2000, the production of tilapia increased and reached domestic and global markets. Major farmed species is Nile tilapia (Oreochromis niloticus), in earthen ponds and cage cultures. Intensification contributed to global tilapia disease outbreaks, with bacterial infections causing mortalities and morbidities, threatening sustainable production. At tilapia farms, high nutrient concentrations, water temperature and fish densities enhance bacterial growth including virulent bacterial clones and potential zoonotic bacteria. Global warming favours this. This review respectively provides a comprehensive overview of the most common and emerging bacterial …

Berry Study Leads To Sweet Faculty Startup, Kateri Hartman

Nebraska Innovation Campus Materials

When the American Aronia Berry Association approached Husker researcher Changmou Xu with a problem, he set out to solve it in a big way. He and his wife and co-founder, Xiaoqing Xie, have worked with numerous campus entities to increase the value of the aronia berry through their startup, A+ Berry. The company is focused on juice products made from aronia berries. Due to their astringency,the berries don’t have an inherent demand; however, they have great potential to impact the Midwest and beyond through their high return value as a crop and impressive health benefits.They boast significantly higher levels of …

Circadian Rhythms Mediate Infection Risk In Daphnia Dentifera, Alaina C. Pfenning-Butterworth, David T. Nguyen, Jessica L. Hite, Clayton E. Cressler

School of Biological Sciences: Faculty Publications

Biological rhythms mediate important within-host processes such as metabolism, immunity, and behavior which are often linked to combating disease exposure. For many hosts, exposure to pathogens occurs while feeding. However, the link between feeding rhythms and infection risk is unclear because feeding behavior is tightly coupled with immune and metabolic processes which may decrease susceptibility to infection. Here, we use the Daphnia dentifera–Metschnikowia bicuspidata host–pathogen system to determine how rhythms in feeding rate and immune function mediate infection risk. The host is known to have a nocturnal circadian rhythm in feeding rate, yet we found that they do not exhibit …