Digital Commons Network^™

Baseline Susceptibility Of Western Corn Rootworm (Coleoptera: Crysomelidae) To Cry3bb1 Bacillus Thuringiensis Toxin, Blair D. Siegfried, Ty T. Vaughn, Terrence A. Spencer

Department of Entomology: Faculty Publications

Susceptibility to Cry3Bb1 toxin from Bacillus thuringiensis (Bt) was determined for western corn rootworm, Diabrotica virgifera virgifera LeConte, neonates from both laboratory and field populations collected from across the Corn Belt. Rootworm larvae were exposed to artificial diet treated with increasing Cry3Bb1 concentrations, and mortality and growth inhibition were evaluated after 4Ð7 d. The range of variation in Bt susceptibility indicated by growth inhibition was similar to that indicated by mortality. Although interpopulation variation in susceptibility was observed, the magnitude of the differences was comparable with the variability observed between generations of the same population. In general, the toxin was …

Farnesol Concentrations Required To Block Germ Tube Formation In Candida Albicans In The Presence And Absence Of Serum, Daniel D. Mosel, Raluca Dumitru, Jacob M. Hornby, Audrey L. Atkin, Kenneth W. Nickerson

Papers in Microbiology

Concentrations of (E,E)-farnesol needed to inhibit germ tube formation were determined for Candida albicans strains A72 and SC5314 by using six different conditions known to trigger germination. For defined media, 1 to 2 μM farnesol was sufficient. However, with serum at 2 to 20%, up to 250 μM farnesol was required. Farnesol blocked germ tube formation but did not block elongation of existing germ tubes.

Grain Quality Of Brazilian Maize Genotypes As Influenced By Nitrogen Level, Aildson P. Duarte, Stephen C. Mason, David S. Jackson, Jorge De C. Kiehl

Department of Food Science and Technology: Faculty Publications

Maize (Zea mays L.) is an important crop in Brazil, and concerns about grain quality are increasingly important with increasing exports and use of grain for specific end-uses. A wide range of genotypes are grown and N application is required to produce high yields. The objectives of these studies were to: (i) determine N application effects on the kernel hardness and breakage susceptibility of a wide range of Brazilian genotypes ranging from dent to flint kernel types and (ii) determine relationships among kernel hardness and breakage susceptibility tests, yield and N and oil concentration. Three studies were conducted with …

Gamma Sigma Delta Newsletter - Nebraska Chapter, Issue #30, August 2005

Gamma Sigma Delta, Nebraska Chapter: Newsletters

CONTENTS:
PRESIDENT’S MESSAGE
IN MEMORIAM
Gamma Sigma Delta Scholarship Recognition
Student Highlight
Gamma Sigma Delta Seminar
GAMMA SIGMA DELTA COMMITTEES, 2004-2005
NOMINATIONS FOR MEMBERSHIP TO GAMMA SIGMA DELTA

Predictors Of First Mental Health Service Utilization Among Homeless And Runaway Adolescents, Terceira A. Berdahl, Dan R. Hoyt, Les B. Whitbeck

Department of Sociology: Faculty Publications

Purpose: To describe and explain variations in first mental health service utilization before and after running away from home for homeless adolescents.

Methods: Survey interviews were conducted with homeless and runaway youth in several Midwestern locations. The effects of family of origin factors and street experiences on the likelihood of seeing a mental health professional for the first time before running away and after running away for the first time were examined. Bivariate and multivariate logistic regression methods are used to analyze these data. Interactions are tested across race and gender sub-groups.

Results: Caretaker education, caretaker rejection, and family transitions …

College Of Agricultural Sciences And Natural Resources: 12th Annual Report August 1, 2004-July 31, 2005

College of Agricultural Sciences and Natural Resources (CASNR): Annual Reports

It is our pleasure to provide the Twelfth Annual Report for the College of Agricultural Sciences and Natural Resources (CASNR). We believe in the importance of reflecting on the accomplishments of our faculty, staff and students. The annual report offers the opportunity to review the year, and serves as an archive of accomplishments for our College. This report covers the period August 1, 2004 to July 31, 2005.

The college enjoyed another year of accomplishments and change. The students excelled, the faculty’s enthusiasm for the academic mission continued to thrive and campus programming expanded. Clearly, the most visible activity this …

Integrating Animal Feeding Strategies Into Cnmp Processes: Role Of Updated Asae Standard D384.2, Richard K. Koelsch, W. Powers, A. L. Sutton

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

This paper introduces the new ASAE Standard D384.2, Manure Production and Characteristics. This new standard provides an equation-based approach that integrates dry matter and nutrient intake as well as animal performance into the final estimate of total solids, nitrogen, and phosphorus excretion for seven livestock and poultry species. The manure excretion estimates of the new standard are compared with the past ASAE standard as well as other commonly used reference values for three specie groups. Significant differences in excretion are common with the new standard compared to past standards. The paper also details examples of how common industry variations in …

Managing Phosphorus In Beef Feeding Operations, William F. Kissinger, Richard K. Koelsch, Galen E. Erickson, Terry J. Klopfenstein

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A commercial feedlot study was conducted to determine manure nutrient flow in six feedlots representing 6,366 cattle. On average, cattle involved in this summary were yearlings (BW = 353 kg) and gained 183 kg over 123 d. It was calculated that 11.5% of the feed nitrogen and 16.9% of the feed phosphorus was retained by the animal with the remaining nutrients excreted. On average, 25.6 kg of N and 4.1 kg of P (DM basis) were excreted per fed beef animal. On average, 887 kg total manure (solids and water) were removed per finished animal (7.2 kg/animal/d) averaging 73% total …

Fertility Of Yearling Beef Bulls During Mating, R. W. Ellis, Gary P. Rupp, P. J. Chenoweth, L. V. Cundiff, D. D. Lunstra

Department of Animal Science: Faculty Publications

Crossbred (Bos taurus) yearling beef bulls were assessed for breeding soundness and physical traits prior to multi-sire natural mating at pasture. Bulls (n = 60) were assigned to six groups of nine or 10 bulls and two bull-groups were rotated on 14-day intervals during a 63-day mating season in each breeding herd (n = 3) of 191–196 cows. The remaining bulls (n = 14) were maintained under similar environmental conditions without mating exposure. Bulls were observed during mating and assessed for breeding soundness and changes following mating. Bulls used for breeding (UFB) lost 77 kg …

Agrohort: Agronomy And Horticulture Growing People First 2005

Department of Agronomy and Horticulture: Annual Newsletters

CONTENTS

Alumni news . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25

Articles:

Agronomy and horticulture highlights . . . . . . . . . . . . . . . . . . . . . . . 5

Barta brothers . . . . . . . . . . . . . . . . . . …

Animal Science Alumni Newsletter, Summer 2005

Department of Animal Science: Departmental News

Contents:

Beef Teaching Herds have Interesting History
From the Department Head’s Desk
Beef Cattle Merchandising Class & Sale
Alum Receives Checkoff’s Distinguished Service Award
Bulls Worth Waiting For
University of Nebraska Represented at National Convention and Trade Show
Greg Ibach Fulfilling His Goal In Serving Agriculture
Department of Agriculture Hires Alum as New State Veterinarian
Serendipitous Couple — Fifth Generation of Farmers
Bud Britton Golf Classic
Block and Bridle Honoree of the Year
Officers and Advisors for 2005-2006
Block & Bridle Awards
Recent Department Awards and Recognitions
Brett White and Merlyn Nielsen Receive Holling Family Teaching Awards
Dedication of Terry …

Nebline, July 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Avoid Portion Distortion With MyPyramid’s Specific Guidelines
Mandevilla — Tropical Vine with Showy Flowers
Fragrance in the Garden
Garden Guide: Things to do this month
Most Leaf Galls Don’t Harm Trees
Traveling This Summer? Find Bedbugs in Hotel Rooms Before They Find You!
Household Hazardous Waste Collections, June 24 & 25
Some Insects Thrive on Rat Poison
P3 Intern Working at Extension
Pine Wilt Disease
How to Sample for Pinewood Nematode
Pine Species to Avoid Planting
Presentation on Sustainable Agriculture, July 8
Predicting the Last Irrigation for Corn, Milo and Soybeans
Irrigating Summer Turf
In Hot Weather, Raise Mower Height …

High-Throughput Immunoblotting Identifies Biotin-Dependent Signaling Proteins In Hepg2 Hepatocarcinoma Cells1, Rocio Rodriguez-Melendez, Jacob B. Griffin, Gautam Sarath, Janos Zempleni

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Biotin affects the abundance of mRNA coding for ~10% of genes expressed in human-derived hepatocarcinoma (HepG2) cells. Here, we determined whether effects of biotin on gene expression are associated with changes in the abundance of distinct proteins in cell signaling and structure. HepG2 cells were cultured in media containing the following concentrations of biotin: 0.025 nmol/L (denoted “deficient”), 0.25 nmol/L (“physiological” = control), and 10 nmol/L (“pharmacological”) for 10 d before harvesting. The abundance of 1009 proteins from whole-cell extracts was quantified by using high-throughput immunoblots. The abundance of 44 proteins changed by at least 25% in biotin-deficient and biotin-supplemented …

Effect Of Bio-Optical Parameter Variability On The Remote Estimation Of Chlorophyll-A Concentration In Turbid Productive Waters: Experimental Results—Erratum, Giorgio Dall'olmo, Anatoly A. Gitelson

School of Natural Resources: Faculty Publications

In the original publication [Dall’Olmo and Gitelson, Applied Optics (2005) 44: 412-422], certain information was presented inaccurately. These inaccuracies are corrected here.

Nebline, June 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Meth Production is Toxic to Communities
2005 Perennial Plant of the Year
Climbing, Twining and Vining
Plant a Moss and Wire Hanging Basket
Itch Mite Update: Extension Will Warn When “Mite Showers” May Happen
Spider Bites? Look for Yellow Sac Spiders
Spider Bites or Skin Infection?
Scouting and Treating for Soybean Rust
The Nebraska LEAD Program
June is Noxious Weed Awareness Month
President’s Notes — Janet’s Jargon
Household Hints: Cleaning Dirty Socks
Take Time for Family Activities
FCE News & Events
Cleaning Supplies Checklist
MyPyramid: The Basics
MVP Pudding with Milk Recipe
Water is a Nutrient, Too
June is Dairy …

Nitrogen Use Efficiency – State Of The Art, Achim R. Dobermann

Department of Agronomy and Horticulture: Faculty Publications

Nitrogenous fertilizers have contributed much to the remarkable increase in food production that has occurred during the past 50 years (Smil, 2001). Globally, however, N fertilizers also account for 33% of the total annual creation of Nr or 63% of all anthropogenic sources of reactive nitrogen (Nr) (Table 1). Reactive nitrogen is defined as all biologically, photochemically, and/or radiatively active forms of N -- a diverse pool of nitrogenous compounds that includes organic compounds (e.g. urea, amines, proteins, amides), mineral N forms, such as NO₃^- and NH₄⁺ as well as gases that are chemically active in …

Surface Display Of Recombinant Proteins On Bacillus Thuringiensis Spores, Cheng Du, Wing C. Chan, Timothy W. Mckeithan, Kenneth W. Nickerson

Papers in Microbiology

The insecticidal protoxin from Bacillus thuringiensis has been shown to be a major component of the spore coat. We have developed a novel surface display system using B. thuringiensis spores in which the N-terminal portion of the protoxin is replaced with a heterologous protein. The expression vector with a sporulationspecific promoter was successfully used to display green fluorescent protein and a single-chain antibody (scFv) gene that encodes anti-4-ethoxymethylene-2-phenyl-2-oxazolin-5-one (anti-phOx) antibody. The spores that carry the anti-phOx antibody can bind to phOx specifically.

Using Ems’S To Improve Compliance On Livestock And Poultry Operations, L. Mark Risse, Richard K. Koelsch, Bill Bland, Elizabeth A. Bird, Thomas M. Bass

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

Across the United States and abroad, innovative producers, processors, trade organizations and others in the agricultural sector are exploring the promise of Environmental Management Systems (EMS) to improve their environmental and business performance. An Environmental Management System or EMS helps farmers develop their own, personal strategies for reducing environmental risk on their operations by integrating environmental management considerations into production management decisions. It is a voluntary, flexible approach and is based on a producer's own sense of how best to manage an operation.

Partnerships for Livestock Environmental Management Systems is a 4-year project to explore the potential of livestock Environmental …

Center For Grassland Studies Newsletter, Spring 2005, Volume 11, No. 2

Center for Grassland Studies: Newsletters

Can Distillers Grains Replace Forage? by Sarah Morris, Jim MacDonald, Don Adams and Terry Klopfenstein, Department of Animal Science, UNL

Nebraska Wildlife Federation Looking for Tallgrass Prairies by Penny Perkins and Duane Hovorka, Nebraska Wildlife Federation

Program Set for 2005 Nebraska Grazing Conference

CGS Associate Leaves Legacy of Native Plantings Along Nebraska Highways

Grazing Livestock Systems Update

Urbanized Buffalograss in Demand

The Leading Object: May 2005

Leading Object

Content:
Perspectives by John C. Owens
UNL’s NAMA Chapter Captures Top Award
Nebraska Food Cooperative in the Works
Biochemistry Professor Honored
Raun’s Career Focused on Helping Others with Farming
Students’ Floral Designs Complement Artwork
Farming Together Workshop Helps Families
Mayo New Interim Associate Dean of ARD
Wagner Receives KUDO
Roeber Takes Post at Academic Affairs
OEA Goes to Larsen
Early Pickup on Display at Tractor Museum
ARDC, Barta Building Celebrations Planned

Agricultural Research Division News --June 2005

Agricultural Research Division: News and Annual Reports

Comments from the Dean- Darrell W. Nelson; ARD Internship Richard Ferguson; Strategic Planning in IANR; Sampson Range and Pasture Management Endowments; IANR Priority Goals 2005-2008; Anna Elliott Proposals; ARD Interdisciplinary Research Grants Program; Recognition of Junior Faculty for Excellence in Research; Layman Awards; ARD Advisory Council Election Results; Undergraduate Honors Research Program; William G. Whitmore Student Travel Endowment; David H. & Annie E. Larrick Fund 2005; ARD Web site has a “New Look” ; Revised UNL Commodity Board Guidelines; New or Revised Projects — March and April 2005; Proposals Submitted for Federal Grants —March and April 2005; Grants and Contracts …

Nebline, May 2005

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Successful Shade Gardening: Key is Proper Plant Choice, Care
Why So Many Weeds?
2005 All-America Roses
Flowers That Tower
Be Good to Your Worms
Winged Termites or Ants?
Thirteen-lined Ground Squirrels
What is Mange?
Soybean Rust is on the Horizon
Effect of Increasing Energy Prices on Farming Costs
Pioneer Farm Award Nominations Due May 1
Buying High-Quality Trees
Conserving Water in the Landscape
NEP Partners with LPS to Teach Nutrition in Teen Parenting Classes
Cocoa-Berry Yogurt Tarts Recipe
Enjoy Beef Nutrition during May, National Beef Month
Mexican Beef Salad Recipe
Fruit Slush Recipe
Spicy Ranch Dressing Recipe
President’s Notes — …

Are Political Orientations Genetically Transmitted?, John R. Alford, Carolyn L. Funk, John R. Hibbing

Department of Political Science: Faculty Publications

We test the possibility that political attitudes and behaviors are the result of both environmental and genetic factors. Employing standard methodological approaches in behavioral genetics—–specifically, comparisons of the differential correlations of the attitudes of monozygotic twins and dizygotic twins—–we analyze data drawn from a large sample of twins in the United States, supplemented with findings from twins in Australia. The results indicate that genetics plays an important role in shaping political attitudes and ideologies but a more modest role in forming party identification; as such, they call for finer distinctions in theorizing about the sources of political attitudes. We conclude …

Identification Of Cis-Acting Elements That Mediate The Replication And Maintenance Of Human Papillomavirus Type 16 Genomes In Saccharomyces Cerevisiae, Kitai Kim, Peter C. Angeletti, Elizabeth C. Hassebroek, Paul F. Lambert

Nebraska Center for Virology: Faculty Publications

Papillomaviruses contain small double-stranded DNA genomes that are maintained in persistently infected mammalian host epithelia as nuclear plasmids and rely upon the host replication machinery for replication. Papillomaviruses encode a DNA helicase, E1, which can specifically bind to the viral genome and support DNA synthesis. Under some conditions in mammalian cells, E1 is not required for viral DNA synthesis, leading to the hypothesis that papillomavirus DNA can be replicated solely by the host replication machinery. This machinery is highly conserved among eukaryotes. We and others found that papillomavirus DNA could replicate in a simple eukaryote, Saccharomyces cerevisiae. Specifically, papillomavirus …

Influence Of Breed, Heterozygosity, And Disease Incidence On Estimates Of Variance Components Of Respiratory Disease In Preweaned Beef Calves, G. D. Snowder, L. Dale Van Vleck, L. V. Cundiff, G. L. Bennett

Department of Animal Science: Faculty Publications

The objective of this study was to characterize genetic and environmental factors influencing bovine respiratory disease (BRD) in beef cattle. Records from nine purebred and three composite breeds and a variety of F1 and three-way crosses, including the progeny of 12 additional different sire breeds produced over a 20-yr period (1983 to 2002), were evaluated for breed and heterozygosity effects on the observed incidence of BRD. Heterozygosity fractions for calves and dams were defined by generalized breed origins: British, Continental, and tropically adapted. Variance components were estimated for each pure and composite breed, and across all breeds and crossbreeds. The …

Temporal Origin Of Nitrogen In The Grain Of Tropical Wet-Season Rice, J. E. Sheehy, M. Mnzava, Kenneth G. Cassman, P. L. Mitchell, A. B. Ferrer, R. P. Robles, P. Pablico

Department of Agronomy and Horticulture: Faculty Publications

The total N in the grain is the integral of the product of the total N absorbed at any instant and the fraction of that N eventually allocated to the grain. We investigated the temporal origin of N in the grain of a wet season rice crop and tested the suitability of ¹⁵N nitrate (NH₄ ¹⁵NO₃) as a label for that purpose. The total N content of rice (Oryza sativa L.) plants was measured by growth analysis throughout the duration of the crop and the measurements were used to calculate the rate of total …

Dynamic Response Indicators Of Heat Stress In Shaded And Non-Shaded Feedlot Cattle, Part 2: Predictive Relationships, R. A. Eigenberg, T. M. Brown-Brandl, John A. Nienaber, G. Leroy Hahn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Summer heat provides stressful conditions for Bos taurus feeder cattle; in extreme instances these conditions can be fatal. One management option is to provide shade structures for feedlot animals. This study was conducted during the summer of 2001 to compare physiological responses of cattle with shade access or no-shade access. Eight steers were selected from a group of 12, and assigned individual pens. Four pens were fitted with shade structures that allowed the steers to choose shade; the remainder had no shade option. The animals were rotated through pen assignments during the summer season. Continuous measures of respiration rate and …

Should I Diet? What Should I Eat? Different Decision Making Factors Predict Dieting Intentions Versus Actual Dietary Behavior, Marc T. Kiviniemi, Kristi E. White, Amy M. Humke

Department of Psychology: Faculty Publications

Many individuals diet to lose weight. Most attempts are not successful—weight is either not lost or is quickly regained.We investigated decision making factors influencing dieting intentions versus those influencing actual behavior. Prior to eating in a university cafeteria, 65 participants reported their cost-benefit beliefs about low fat foods and fruits/vegetables, affective associations with both food categories, and whether they were currently dieting to lose weight. After the meal, participants reported what they ate. Using these reports and nutritional data from the college dining service, we computed the calories, total and saturated fat, sodium, and cholesterol consumed. Cost-benefit beliefs and affective …

Current Mood And Food Choices: The Relation Between Positive Affect And Dietary Behavior, Kristi E. White, Amy M. Humke, Marc T. Kiviniemi

Department of Psychology: Faculty Publications

Colloquially, much is made of the relation between food and mood. For example, “comfort foods” are eaten in the service of affect regulation. Affect can influence behavioral choices in a number of ways. We investigated whether college students’ mood when making dietary choices influenced the nutritional value of the foods chosen. Sixty-five participants completed a questionnaire immediately prior to eating a meal in a college dining hall. The questionnaire included a measure of current positive affect. Following the meal, participants reported what they ate for dinner. Using nutritional data from the college dining services, we used participants’ self reported food …

Department Of Biological Systems Engineering Newsletter, Issue 1, April 2005

BSE Department Magazine

Contents:
Welcome: Comments from Department Head
New Lab in BSE
Dr. Martin Serves as BSE Interim Head
Splinter Labs
New Department Head
Hello-Goodbye
Faculty and Staff News
Cliff Hunter Memorial Picnic
Student News
Quarter-scale Tractor Team
Freshmen Eat Cars
Scholarship Recognition
Annual Spring Recognition Banquet
New Graduates
Generous Gifts to Endowments in BSE