Digital Commons Network^™

Evaluation Of In Vitro Gas Production And Rumen Bacterial Populations Fermenting Corn Milling (Co)Products, W. L. Williams, L. O. Tedeschi, Paul J. Kononoff, T. R. Callaway, S. E. Dowd, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the fermentation dynamics of 2 commonly fed corn (co)products in their intact and defatted forms, using the in vitro gas production (IVGP) technique, and to investigate the shifts of the predominant rumen bacterial populations using the 16S rDNA bacterial tag-encoded FLX amplicon pyrosequencing (bTEFAP) technique. The bTEFAP technique was used to determine the bacterial profile of each fermentation time at 24 and 48 h. Bacterial populations were identified at the species level. Species were grouped by substrate affinities (guilds) for cellulose, hemicellulose, pectin, starch, sugars, protein, lipids, and lactate. The 2 (co)products …

Evaluation Of Protein Fractionation And Ruminal And Intestinal Digestibility Of Corn Milling Co-Products, J. M. Kelzer, Paul J. Kononoff, L. O. Tedeschi, T. C. Jenkins, K. Karges, M. L. Gibson

Department of Animal Science: Faculty Publications

Novel corn milling co-products developed from technological advancements in ethanol production vary widely in chemical composition and nutrient availability. The objectives of this study were to characterize feed protein fractions and evaluate differences in rumen-undegradable protein (RUP) and its digestible fraction (dRUP), amino acid concentration, and in vitro gas production of 7 corn milling co-products. The crude protein (CP; % of dry matter) of co-products was 12.7 for germ, 26.9 for dried distillers grains plus solubles that had no heat exposure before fermentation (DDGS1), 45.4 for highprotein dried distillers grains (HPDDG), 12.7 for bran, 30.2 for wet distillers grains plus …

Utilization Of Nitrogen In Cows Consuming Wet Distillers Grains With Solubles In Alfalfa And Corn Silage-Based Dairy Rations, A. M. Gehman, Paul J. Kononoff

Department of Animal Science: Faculty Publications

The objectives of this experiment were to determine the effects of forage type on nutrient digestibility, purine derivative excretion, nitrogen utilization, and milk production in dairy cattle consuming rations containing high levels of wet distillers grains with solubles (WDGS). Primiparous (n = 8) and multiparous (n = 20) Holstein cows were used in a replicated 4 × 4 Latin square. Animals were fed 1 of 4 treatments during each 21-d period: 1) CONT-CS, 0% WDGS and high corn silage; 2) CONT-AS, 0% WDGS and high alfalfa silage; 3) WDGS-CS, 25% WDGS and high corn silage; and 4) WDGS-AS, 25% WDGS …

Effect Of Dietary Crude Protein Source On Hormone And Follicle Characteristics In Beef Heifers, J. L. Martin, D. M. Larson, H. L. Stroh, Andrea S. Cupp, Richard N. Funston

Department of Animal Science: Faculty Publications

Ground, raw soybeans (SB), or dried distillers grain plus solubles (DDGS) were utilized in heifer development diets to determine the effect of dietary fat and protein source on hormone and follicle characteristics and ADG. The experiment was conducted over 2 yr with 100 June-born heifers (199 ± 2 kg initial BW, n = 50 per yr). The experimental periods were 157 and 207 d in yr 1 and 2, respectively. Heifers were provided a dietary supplement (DM basis) of 1.23 kg of SB and 0.40 kg of corn or 1.65 kg of DDGS between weaning and breeding. Estrus was synchronized …

Economic Effects Of The Consumer-Oriented Gm Products In Markets With A Labeling Regime, Rim Lassoued, Konstantinos Giannakas

Cornhusker Economics

One of the most intriguing attributes of modern industrial society is its approach to nutrition. Humanity has moved from subsistence economies, where eating was a matter of survival, to economies characterized by the existence of an ever increasing variety of food products. An important recent addition to the types of food products included in our diet has been that of genetically modified (GM) products.

Despite their important agronomic benefits to agricultural producers (e.g., increased yields and/or reduced input costs), GM products have been facing a rather strong consumer opposition. Fears related to potential health and environmental effects of genetic modification, …

Toward Sustainability In Business, Industry And Community: Empathy Tempered Choices, Prabhakar Shrestha, Gary D. Lynne

Cornhusker Economics

In today’s world of 24-hour news service on the internet, youtube, facebook, twitter, electronic newspapers, etc., there are many sources of information constantly seeking our attention, triggering our response and affecting our psyche. Where are such habits of tuning into internet services, mobile services, etc., leading us? Jeremy Rifkin in his new book, The Empathic Civilization talks about the dawn of a new Third Industrial Civilization. The main feature is the sharing of information among millions of people, which is shaping the spatial and temporal orientation of human civilization. This also leads to greater differentiation of skills, greater connectivity, extension …

Mzm1 Influences A Labile Pool Of Mitochondrial Zinc Important For Respiratory Function, Aaron Atkinson, Oleh Khalimonchuk, Pamela Smith, Hana Sabic, David Eide, Dennis R. Winge

Department of Biochemistry: Faculty Publications

Zinc is essential for function of mitochondria as a cofactor for

several matrix zinc metalloproteins. We demonstrate that a

labile cationic zinc component of low molecular mass exists in

the yeast mitochondrial matrix. This zinc pool is homeostatically

regulated in response to the cellular zinc status. This pool

of zinc is functionally important because matrix targeting of a

cytosolic zinc-binding protein reduces the level of labile zinc

and interferes with mitochondrial respiratory function. We

identified a series of proteins that modulate the matrix zinc

pool, one of which is a novel conserved mitochondrial protein

designated Mzm1. Mutant mzm1∆ cells …

Brown Midrib Mutations And Their Importance To The Utilization Of Maize, Sorghum, And Pearl Millet Lignocellulosic Tissues, Scott E. Sattler, Deanna L. Funnell-Harris, Jeffrey F. Pedersen

Department of Plant Pathology: Faculty Publications

Brown midrib mutants have been isolated in maize (Zea mays), sorghum (Sorghum bicolor) and pearl millet (Pennisetum glaucum) arising by either spontaneous or chemical mutagenesis. The characteristic brown coloration of the leaf mid veins is associated with reduced lignin content and altered lignin composition, traits useful to improve forage digestibility for livestock. Brown midrib phenotype is correlated with two homologous loci in maize (bm1 and bm3) and sorghum (bmr6 and bmr12), which encode cinnamyl alcohol dehydrogenase (CAD) and a caffeic O-methyl transferase (COMT). These enzymes are involved in the last …

Regulation Of Septum Formation By The Bud3–Rho4 Gtpase Module In Aspergillus Nidulans, Haoyu Si, Daniela Justa-Schuch, Stephan Seiler, Steven D. Harris

Department of Plant Pathology: Faculty Publications

The ability of fungi to generate polarized cells with a variety of shapes likely reflects precise temporal and spatial control over the formation of polarity axes. The bud site selection system of Saccharomyces cerevisiae represents the best-understood example of such a morphogenetic regulatory system. However, the extent to which this system is conserved in the highly polarized filamentous fungi remains unknown. Here, we describe the functional characterization and localization of the Aspergillus nidulans homolog of the axial bud site marker Bud3. Our results show that AnBud3 is not required for polarized hyphal growth per se, but is involved in septum …

Fluid Fertilizer’S Role In Sustaining Soils Used For Bio-Fuels Production, John L. Kovar, Douglas L. Karlen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The short- and long-term effects on soil nutrient cycling, physical properties, and biological activity of striving for higher grain yields and removing crop residues for bio-fuels production must be understood to provide more quantitative crop and soil management guidelines. This study focuses primarily on potassium (K) and sulfur (S) response by corn (Zea mays L.) grown for bio-energy feedstock production. Our objectives for 2009 were to evaluate (i) the performance of several S fertilizers, including liquid ammonium thiosulfate (12-0-0-26S), for corn grown in Iowa, and (ii) the use of surface or subsurface bands of N-P-K-S fluid fertilizers to optimize …

Procedures For Publishing In Non-Usda Media (Outside Publishing)

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This P&P is an update of a previous version dated March 10, 1998. This P&P outlines revised responsibilities of the ARS author, Research Leader (RL), and other ARS officials. The most salient features of the new P&P are that the manuscript approval authority and associated responsibilities are delegated to the RL. ARS Form 115 (ARS-115) is revised accordingly (see Exhibit 1). Among the essential RL responsibilities are to determine the need and method for manuscript peer review, approve the technical and interpretive content of the manuscript, notify upper management about manuscripts containing prominent issues, obtain proper clearance/approval from upper management …

U.S. Meat Animal Research Center Clay Center, Ne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mission

Research Units

U.S. Meat Animal Research Center Animal Health Research Unit

U.S. Meat Animal Research Center Environmental Management Research Unit

U.S. Meat Animal Research Center Genetics & Breeding Research Unit

U.S. Meat Animal Research Center Meat Safety and Quality Research Unit

U.S. Meat Animal Research Center Nutrition Research Unit

U.S. Meat Animal Research Center Reproduction Research Unit

Nutrient Removal As A Function Of Corn Stover Cutting Height And Cob Harvest, Jane M. F. Johnson, Wally W. Wilhelm, Douglas L. Karlen, David W. Archer, Brian J. Wienhold, David T. Lightle, David Laird, John Baker, Tyson E. Ochsner, Jeff M. Novak, Ardell D. Halvorson, Francisco Arriaga, Nancy Barbour

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

One-pass harvest equipment has been developed to collect corn (Zea mays L.) grain, stover, and cobs that can be used as bioenergy feedstock. Nutrients removed in these feedstocks have soil fertility implication and affect feedstock quality. The study objectives were to quantify nutrient concentrations and potential removal as a function of cutting height, plant organ, and physiological stage. Plant samples were collected in 10-cm increments at seven diverse geographic locations at two maturities and analyzed for multiple elements. At grain harvest, nutrient concentration averaged 5.5 gN kg⁻¹, 0.5 gP kg⁻¹, and 6.2 gK kg^{−1 …}

Effects Of A New Waste-Processing By-Product On Soil And Vegetation At Fort Campbell, Tennessee, R. R. Busby, D. L. Gebhart, H. A. Torbert, J. O. Dawson, G. A. Bollero, K. N. Potter, D. R. Curtin

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A garbage-processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. A study was initiated to evaluate the uncomposted organic by-product of this process as a soil amendment for establishing native prairie grasses on disturbed Army training lands. The waste was incorporated into a silt loam soil at Fort Campbell Military Reservation in the central United States. The waste material was applied at rates of 0, 4.5, 9, 18, and 36 Mg ha^-1 and seeded with native prairie grasses to assess its effects on vegetation for two growing seasons, with an additional …

Multispectral Imaging Systems For Airborne Remote Sensing To Support Agricultural Production Management, Yanbo Huang, Steven J. Thomson, Yubin Lan, Stephan J. Maas

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This study investigated three different types of multispectral imaging systems for airborne remote sensing to support management in agricultural application and production. The three systems have been used in agricultural studies. They range from low-cost to relatively high-cost, manually operated to automated, multispectral composite imaging with a single camera and integrated imaging with custom-mounting of separate cameras. Practical issues regarding use of the imaging systems were described and discussed. The low-cost system, due to band saturation, slow imaging speed and poor image quality, is more preferable to slower moving platforms that can fly close to the ground, such as unmanned …

Primary Transcriptomes Of Mycobacterium Avium Subsp. Paratuberculosis Reveal Proprietary Pathways In Tissue And Macrophages, Harish K. Janagama, Elise A. Lamont, Sarah B. George, John P. Bannantine, Wayne W. Wayne, Zheng J. Tu, Scott J. Wells, Jeremy Schefers, Suelee Robbe-Austerman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Mycobacterium avium subsp. paratuberculosis (MAP) persistently infects intestines and mesenteric lymph nodes leading to a prolonged subclinical disease. The MAP genome sequence was published in 2005, yet its transcriptional organization in natural infection is unknown. While prior research analyzed regulated gene sets utilizing defined, in vitro stress-related or advanced surgical methods with various animal species, we investigated the intracellular lifestyle of MAP in the intestines and lymph nodes to understand the MAP pathways that function to govern this persistence.

Results: Our transcriptional analysis shows that 21%, 8% and 3% of the entire MAP genome was represented either inside tissues, …

Effects Of Forage Quality And Cell Wall Constituents Of Bermuda Grass On Biochemical Conversion To Ethanol, William F. Anderson, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Paul J. Weimer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Bermuda grass is an attractive candidate as a feedstock for biofuel production because over four million hectares of Bermuda grass are already grown for forage in the Southern USA. Because both rumen digestion and biochemical conversion to ethanol depend upon enzymatic conversion of the cell wall polysaccharides into fermentable sugars, it is probable that grasses bred for increased forage quality would be more amenable for ethanol production. However, it is not known how variation in rumen digestibility and cell wall/fiber components correlates with efficiency of conversion to ethanol via fermentation. The objective of this research was to determine relationships between …

Methods For Assessing Fish Populations, Kevin L. Pope, Steve E. Lochmann, Michael K. Young

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Fisheries managers are likely to assess fish populations at some point during the fisheries management process. Managers that follow the fisheries management process (see Chapter 5) might find their knowledge base insufficient during the steps of problem identification or management action and must assess a population before appropriate actions can be taken. Managers will implement some type of assessment during the evaluation step as a means of measuring progress relative to objectives. Choosing how to assess a population is an important decision because managers strive to maximize their knowledge of a population while minimizing the time and money expended to …

Understanding The Science Of Climate Change Talking Points – Impacts To Alaska Maritime And Transitional, Caroline Jezierski, Rachel Loehman, Amanda Schramm

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Alaska is a huge state spanning 375 million acres and occupying nearly one-fifth of the land area for the contiguous 48 states. More than half of the coastline of the entire United States is in Alaska. Due to the great size and geographically diverse nature of Alaska, two bioregional documents were produced: “Boreal and Arctic” and “Alaska Maritime and Transitional.” In Alaska, the vast majority of the land is public; with approximately 222 million acres (approximately 60%) designated federal lands and another 90 million acres (approximately 24%) in state ownership. There are 17 National Park Service (NPS) areas in Alaska …

Understanding The Science Of Climate Change Talking Points – Impacts To Alaska Boreal And Arctic, Caroline Jezierski, Rachel Loehman, Amanda Schramm

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Alaska is a huge state spanning 375 million acres and occupying nearly one-fifth of the land area of the contiguous 48 states. More than half of the coastline of the entire United States is in Alaska. Due to the great size and geographically diverse nature of Alaska, two bioregional documents were produced: “Boreal and Arctic” and “Alaska Maritime and Transitional.” In Alaska, the vast majority of the land is public; with approximately 222 million acres (approximately 60%) designated federal lands and another 90 million acres (approximately 24%) in state ownership. There are 17 National Park Service (NPS) areas in Alaska …

2010 Spring Seed Book, Nebraska Crop Improvement Association

Nebraska Crop Improvement Association Seed Guides

FOUNDATION SEED

PLANT VARIETY PROTECTION ACT and HOW IT BENEFITS YOU!

TURFGRASSES

PERENNIAL FORAGE GRASSES

SPRING WHEAT

SPRING BARLEY

OAT VARIETY CHARACTERISTICS - 2010

OATS

MILLET

HYBRID SEED CORN

NEBRASKA SEED QUALITY ASSURANCE® PROGRAM

SOYBEANS BUYERS’ NOTICE

APPROVED SEED CONDITIONERS

CUSTOM CERTIFIED CONDITIONERS

2009 NCIA MEMBERS

Emergence Of Diseases From Wildlife Reservoirs, J. C. Rhyan, T. R. Spraker

Michigan Bovine Tuberculosis Bibliography and Database

Interest in the epidemiology of emerging diseases of humans and livestock as they relate to wildlife has increased greatly over the past several decades. Many factors, most anthropogenic, have facilitated the emergence of diseases from wildlife. Some livestock diseases have ‘‘spilled over’’ to wildlife and then ‘‘spilled back’’ to livestock. When a population is exposed to an infectious agent, depending on an interaction of factors involving the host, agent, and environment, the population may be resistant to infection or may become a dead-end host, a spillover host, or a maintenance host. Each exposure is unique; the same species of host …

The Leading Object: May 2010, John C. Owens

Leading Object

Perspectives; NU delegation visits Vietnam; Green to assume vice chancellorship July 19; Pioneer Hi-Bred researcher credits success to ‘solid education’; Presidential nominee has IANR ties; Awards ... Dinsdale and OEA; Water research to ‘jump start’ new center; Entrepreneurship, ag are tour highlights; Death

Recent Changes In Density, Biomass, Recruitment, Size Structure, And Nutritional State Of Dreissena Populations In Southern Lake Michigan, Thomas F. Nalepa, David L. Fanslow, Steven A. Pothoven

United States Department of Commerce: Staff Publications

Trends in density, biomass, population structure, and nutritional state of Dreissena polymorpha and Dreissena rostriformis bugensis were examined in southern Lake Michigan between the 1990s and 2008. Density and biomass of D. polymorpha increased to a peak in the early 2000s and then declined. In contrast, D. rostriformis bugensis was first found in the southern basin in 2001 and has continued to increase in density or biomass at all depths ever since. In 2008, maximum mean density of D. rostriformis bugensis occurred at 16–30 m (19,000/m²), but maximum biomass (AFDW) occurred at 31–50 m (43.9 g/m²). …

Race And Outcomes Of Autologous Hematopoietic Cell Transplantation For Multiple Myeloma, Parameswaran N. Hari, Navneet S. Majhail, Mei-Jie Zhang, Anna Hassebroek, Fareeha Siddiqui, Karen Ballen, Asad Bashey, Jenny Bird, Cesar O. Freytes, John Gibson, Gregaory Hale, Leona Holmberg, Ram Kamble, Robert A. Kyle, Hillard M. Lazarus, Charles F. Lemaistre, Fausto Loberiza, Angelo Maiolino, Philip L. Mccarthy, Gustavo Milone, Nancy Omondi, Donna E. Reece, Matthew Seftel, Michael Trigg, David Vesole, Brendan Weiss, Peter Wiernik, Stephanie J. Lee, J. Douglas Rizzo, Paulette Mehta

United States Department of Veterans Affairs: Publications

Blacks are twice as likely to develop and die from multiple myeloma (MM), and are less likely to receive an autologous hematopoietic-cell transplant (AHCT) for MM compared to Whites. The influence of race on outcomes of AHCT for MM is not well described. We compared the probability of overall survival (OS), progression- free survival (PFS), disease progression, and nonrelapse mortality (NRM) among Black (N=303) and White (N=1892) recipients of AHCT for MM, who were reported to the Center for International Blood and Marrow Transplant Research (CIBMTR) from 1995 to 2005. The Black cohort was more likely to be female, and …

Time And Degree Of Glycemic Derangement Are Associated With Increased Mortality In Trauma Patients In The Setting Of Tight Glycemic Control, Michael G. Corneille, Celina Villa, Steven Wolf, Joel E. Michalek, Inkyung Jung, Charles E. Wade, John G. Meyers, Daniel L. Dent, Deborah Mueller, Ronald M. Stewart

United States Army: Publications

BACKGROUND: Tight glucose control (TGC) may reduce mortality in critically ill trauma patients. We hypothesize that euglycemia is beneficial, and a measure considering time and degree of hyperglycemia is most associated with mortality.

METHODS: We performed a review of intensive care unit trauma patients admitted for more than 3 days between January 2005 and December 2007 on a TGC protocol with a goal of 80 to 110 mg/dL. Hyperglycemic, hypoglycemic, and euglycemic time ranges, and area of interpolated curves above and below 80 to 110 mg/dL were assessed. Associations with mortality were based on logistic regression models adjusted for age, …

Activity Of Topical Antimicrobial Agents Against Multidrug-Resistant Bacteria Recovered From Burn Patients, Jessie S. Glasser, Charles H. Guymon, Katrin Mende, Steven E. Wolf, Duane R. Hospenthal, Clinton K. Murray

United States Army: Publications

Background:

Topical antimicrobials are employed for prophylaxis and treatment of burn
wound infections despite no established susceptibility breakpoints, which are becoming vital in an era of multidrug-resistant (MDR) bacteria. We compared two methods of determining topical antimicrobial susceptibilities.

Methods:

Isolates of Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA), extended spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae, and Acinetobacter baumanii-calcoaceticus (ABC) from burn patients were tested using broth microdilution and agar well diffusion to determine minimum inhibitory concentrations (MICs) and zones of inhibition (ZI). Isolates had systemic antibiotic resistance and clonality determined. MDR included resistance to antibiotics in three or more …

University Of Nebraska- Lincoln: Fact Book 2009-2010

University of Nebraska-Lincoln Administration: Reports, Papers, Publications, and Presentations

Fact Book Table of Contents

General Information

UNL Core Values ....................................................................... 4

Role and Mission Statement .............................................................................. 5

Institutional & Professional Accreditations ........................................................... 8

UNL Organizational Chart ............................................................................... 10

Student Credit Hours

Fall SCH Since 1979 .......................................................................... 11

SCH by College and Student Level, Fall & Spring Semesters, 5 Year Trend ...... 12

SCH by College and Student Level, Fall Semester, 5 Year Trend .................... 13

SCH by College and Student Level, Spring Semester, 5 Year Trend ................. 14

Summer Sessions Student Credit Hours ............................................ 15

Retention, Degrees and Majors

Student Retention & Graduation Rate Analysis ................................................. 16

Total Degrees …

Confirmatory Factor Analysis Of The Child Feeding Questionnaire Among Low-Income African American Families Of Preschool Children, Richard E. Boles, Timothy D. Nelson, Leigh A. Chamberlin, Jessica M. Valenzuela, Susan N. Sherman, Susan L. Johnson, Scott W. Powers

Department of Psychology: Faculty Publications

This study examined the factor structure for three of the Child Feeding Questionnaire (CFQ) subscales, a widely used measure of parental feeding practices, among 296 low-income parents of African American preschool children. Confirmatory factor analysis showed an overall poor fit among CFQ subscales; Restriction, Pressure to Eat, and Concern about Child Weight, (χ2, (df = 87 = 300.249, CFI = 1.00, NNFI = 1.07, RMSEA = .091). Additionally, Cronbach’s Alpha coefficients for 2 of the three subscales were below acceptable recommendations (Restriction = 0.69; Pressure to Eat = 0.58). These results suggest further psychometric clarification is needed to understand commonly …

Methionine-R-Sulfoxide Reductases And Biological Importance Of Free Methionine Sulfoxide Reduction, Byung Cheon Lee

Department of Biochemistry: Dissertations, Theses, and Student Research

We identified and functionally characterized yeast fRMsr homolog which showed specificity for reduction of free Met-R-SO and contributed to oxidative stress resistance of yeast cells. We further identified three conserved Cys which participate in catalysis through disulfide exchange. Evolutionary analyses revealed that the occurrence of fRMSr is restricted to unicellular organisms. We further found that mammalian MsrA can reduce free Met-S-SO, whereas MsrBs do not reduce free Met-R-SO. Consistent with these findings and the lack of fRMsr, mammalian cells could not grow in media that replaced Met with Met-R-SO. However, they grew in the presence of free Met-S-SO, which was …