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Nebline, May 2023

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

The 4-H Thriving Model Provides a Guide for Helping Youth Grow to Their Full Potential by Tracy Anderson

Beef: A Healthy and Delicious Choice for Your Next Meal by Kayla Colgrove

Recipe of the Month: Skillet Lasagna by Kayla Colgrove

Managing Chlorosis in Trees by Sarah Browning

Garden Guide: Things to Do This Month

May is the Perfect Time to Learn More About Your Drinking Water! by Becky Schuerman

Cash Rent Survey

Challenging Behavior Corner: Providing Choices by Hayley Jackson

Pollinator Spotlight: The Yucca Moth by Kait Chapman

At Ag Literacy Festival, 4th Graders Learn How Agriculture Impacts Their Daily …

Student Perspectives Of Sustainable Transportation Use On A College Campus, Brynn Fuelberth

Department of Environmental Studies: Undergraduate Student Theses

As climate change grows in relevance in society, attention has often been turned to large universities to look at what solutions can help minimize the impacts contributing to the devastation. Sustainable transportation is an alternative that can help combat these issues. This study specifically focuses on what sustainable transportation looks like at the University of Nebraska-Lincoln (UNL), and what barriers and motivations are present in the undergraduate students’ eyes. This was investigated through an open-ended qualitative survey administered to the undergraduate population of the UNL. This study asks how students make their mode decisions in relation to sustainable transportation methods, …

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …

Exploring The Role Of Microrna-1 (Mir-1) On Skeletal Muscle Hypertrophy, Shengyi Fei

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Skeletal muscle hypertrophy is a complex process that involves a range of signaling pathways and transcriptional regulators. Many hormones and growth factors can activate key signaling pathways, such as the PI3K/AKT/mTOR, MAPK, and cAMP pathways, which play a crucial role in the regulation of muscle hypertrophy. In Chapter 1, we reviewed some of the hormones and growth factors known to be associated with skeletal muscle hypertrophy, as well as the function of these key signaling pathways, and revealed some unresolved issues. In Chapter 2, we explored the role of microRNA-1 (miR-1) in skeletal muscle hypertrophy and aimed to determine the …

Gene Expression Changes Linked To Phenylpropanoid-Based Resistance To Fusarium Head Blight Of Wheat, Shiv Singla

Department of Plant Pathology: Dissertations, Theses, and Student Research

Fusarium graminearum is a devastating pathogen of wheat that causes Fusarium head blight (FHB) and contaminates the grain with the mycotoxin deoxynivalenol (DON). Resistance to FHB is quantitative and it is important to identify additional genes conferring resistance against it. The goal of this thesis was to examine if the constitutive expression of two sorghum phenylpropanoid pathway genes, SbCCoAOMT (encoding caffeoyl-CoA O-methyltransferase) and SbC3’H (encoding p-coumarate 3-hydroxylase), in the moderately-susceptible spring wheat CB037 can provide Type-I and Type-II resistance to F. graminearum and determine the underlying mechanisms of the enhanced resistance. The constitutive expression lines (CCoAOMT413, CCoAOMT421, C3H112, …

Optimizing Soil Nutrient Management To Improve Dry Edible Bean Yield And Protein Quality, Emily Jundt

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Soil nutrient management is of the utmost importance to ensure crop yields are maximized to feed the world’s growing population. Micro and macronutrient deficiencies can be detrimental to plant growth and cause a drastic reduction in yield. Optimization of nutrient management needs extensive research. Nutrients such as nitrogen increase crop yield, even in legumes capable of using a large amount of nitrogen (N) via biological N fixation. Iron (Fe) fertilizers are also crucial for legumes as they are susceptible to Fe deficiency induced chlorosis.

Dry edible beans (Phaseolus vulgaris) are leguminous plants and excellent dietary protein sources. Great …

The Presence Of Childhood Obesity In Nebraska And The Physiological Repercussions Of The Disease, Madison R. Bezousek

Honors Program: Senior Projects (Public)

Childhood Obesity can cause lifelong repercussions in children and adults. There has been stigma around the causes of obesity and its relation to lifestyle choices, without consideration of the genetic and syndromic causes. In this literature review the causes of obesity were investigated, along with the effect on the physiological systems and the enviornmental factors that are continuing the obesity epidemic. Preventing and treating lifestyle obesity is something that is seen to have the greatest effect on youth, especially with intergenerational obesity. Nebraska schools have implemented programs to encourage healthy living, and ongoing research is being implemented to reduce the …

Us Public School Demand For Locally Produced Meat And Poultry, Shara Akat

Department of Agricultural Economics: Dissertations, Theses, and Student Research

The objective of the USDA Farm-to-School program is to promote the understanding and use of local food in school education and meals. The hope is that by reconnecting students with how food is raised and purchasing the product from local producers it will increase nutrition and reduce food insecurity and obesity among students while simultaneously increasing economic activity for local producers. Most studies evaluating the federal program have focused on how the economic outcomes for local producers or student related health outcomes and primarily using fresh fruits and vegetables. Fundamental to either of these outcomes is understanding the schools’ demand …

The Role And Implications Of Negotiation In Fed Cattle Transactions, Taralee Hudson

Department of Agricultural Economics: Dissertations, Theses, and Student Research

Within the past three decades, a significant evolution has occurred in how fed cattle are bought and sold. Driven by changes in the quality of beef and consumer health preferences, the beef industry began to advocate for “value-based marketing,” resulting in the development of grid pricing and eventually formula transactions. Alternative marketing arrangements (AMAs) have become the predominant method used for the sale of fed cattle, ultimately resulting in fewer transactions in the negotiated cash market. Such market conditions have recently reignited concerns among industry stakeholders about potentially uncompetitive outcomes for beef producers, particularly after the occurrence of sequential Black …

Women In Conflict: The Psychological Effect Of Propaganda In Conflict, Elizabeth Valerio-Boster

Honors Program: Senior Projects (Public)

In conflicts across the world, propaganda is used to encourage people to support causes whether than be freedom, revolution, or political or economic changes. Previous research has shown that propaganda that targets preexisting notions is particularly effective. Women have been found to be particularly susceptible to propaganda that has emotional implications. My research has been conducted to discover if propaganda that is centered around female empowerment is more effective in getting women to participate in conflict. I use accounts from women participating in conflicts to learn about the roles they play, and the number of women involved. These numbers are …

The Importance Of Mood And Motivation Of An Individual In The Success Of Physical Therapy, Spencer Wagner

Honors Program: Senior Projects (Public)

Physical therapy is a health profession that helps people improve their movement and range of motion in certain body areas. Since the time it was founded, physical therapists have mainly focused on the physical health of the patient. While that is their job, they may not have been as successful because they neglected the mental health of the patient. The mindset of an individual, particularly their mood and motivation, can influence how they progress through physical therapy and how successful they are in regaining functionality in their affected body area. The purpose of this study is to determine how the …

Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo

Honors Program: Senior Projects (Public)

Nitrogen plays a major role in the proper growth and development of maize and is therefore essential to crop production, being the most critical nutrient for achieving optimal yield. In previous field and greenhouse studies, we have found maize lines that differ in their resiliency to nitrogen stress. To identify the potential genomic regions associated with the differences in nitrogen deficiency resilience, Genome-Wide Association Studies (GWAS) were conducted. Based on a previous study, the most consistently resilient maize inbred lines within the Goodman-Buckler diversity panel have been identified as the lines A619 and A661, while the non-resilient inbred lines have …

An Exploration Of Influences On Consumers' Behaviors/Decisions In Relation To Animal Agriculture, Abigail Durheim

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

Animal agriculture provides many daily resources for consumers around the globe. While the average American is at least three generations removed from agriculture, they still hold power to influence the industry with the dollar and their vote. The purpose of this study was to identify influences on consumer behaviors/decisions in relation to animal agriculture. Seven hundred four complete and usable responses were collected via a researcher-developed online survey. Using branding concepts as a framework, the first study evaluated how visual stimuli influenced consumers' intention to purchase Nebraska beef products. Researchers found that visual stimuli did influence respondents to choose Nebraska …

The Impact Of An Inquiry-Based Learning Professional Development Has On Science And Agricultural Teachers' Intent To Use Animal Science Concepts As A Context For Teaching Science, Kasey Harmon

Department of Agricultural Leadership, Education, and Communication: Dissertations, Theses, and Student Research

There is an emerging need for Tennessee and Nebraska high school students to increase their science proficiency. It is important that students are science literate to equip them with basic science skills needed for the workforce. Science literacy enables students to problem solve efficiently, appropriately assess societal issues, and make informed decisions. Inquiry-based learning (IBL) is a teaching strategy that has been shown to positively impact students’ ability to critically think and make informed decisions. Since IBL and agricultural education are centered around experiential learning, combining them gives students the opportunity to learn science in a real-world context. However, previous …

Winter Hardy Small Cereals For Grazing Or Silage In Eastern Nebraska, Abigail M. Sartin

Department of Animal Science: Dissertations, Theses, and Student Research

Two, 3-year studies were conducted to evaluate the value of cereal rye, winter wheat, and winter triticale as forage sources for either for silage or early spring grazing. For silage, the objective was to evaluate species and maturity effects on dry matter yield, nutritive value, and fermentation of small cereal silage. There are no major differences between wheat, rye, or triticale in terms of the relative timing of each species reached each maturity stage. There are no differences among species in dry matter (DM) yield, except for wheat which tends to decline at soft dough due to senescence of the …

Methods To Reduce Nitrogen And Carbon Losses From Finishing Beef Cattle, Hanna K. Cronk

Department of Animal Science: Dissertations, Theses, and Student Research

A finishing experiment evaluated the effect of incremental supplemental rumen-protected lysine on beef cattle performance. All cattle were fed the same diet of high moisture corn, dried rolled corn, modified distillers grains plus solubles, corn silage, and supplement with inclusion levels of lysine ranging from 0 to 7 grams/day, in 1 g increments. Dry matter intake (P ≤ 0.04) and ADG (P ≤ 0.04) decreased linearly as supplemental lysine increased in the diet with no effect on final carcass adjusted feed efficiency (P ≥ 0.34). In the initial 64 days of the experiment, increasing lysine in the …

The Evaluation Of Encapsulated Megasphaera Elsdenii In An Accelerated Beef Step-Up Program And An Acidosis Challenge Model And The Evaluation Of Ramp Versus A Traditional Forage Grain Adaptation Strategy On Methane And Respired Carbon Dioxide, Cindy D. Mansfield

Department of Animal Science: Dissertations, Theses, and Student Research

A metabolism experiment (Exp 1) was conducted to evaluate daily feeding of encapsulated Megasphaera elsdenii (M. elsdenii) NCIMB 41125 along with a one-time drench of 1×10¹¹ CFU of M. elsdenii on dry matter intake (DMI), in-vitro lactate utilization, volatile fatty acid (VFA), and lactate concentration. Treatments consisted of steers which were fed no M. elsdenii (CONTROL), steers drenched with the commercial dose 1×10¹¹ CFU of M. elsdenii (LactiproNXT) on d 1 of the experiment and received no other M. elsdenii (DRENCH), and steers drenched with a commercial dose of LactiproNXT on d …

Comfort Club, Cecilia Duong, Kathy Ho

Honors Program: Expanded Learning Clubs

There are many cases where children are dependent or heavily reliant on a particular item, whether a blanket, plushie, or nightlight. Therefore, Comfort Club aims to create a safe space for children to develop connections with emotionally supportive items that can be taken home and continuously nurtured beyond the classroom. Students also experience the impact of positivity and appreciation within the school as they learn how to effectively work with others, receive optimistic statements from their club leaders, and place their creativity into projects catering to their plushies.

A Deep Learning Framework For Processing And Classification Of Hyperspectral Rice Seed Images Grown Under High Day And Night Temperatures, Víctor Díaz-Martínez, Jairo Orozco-Sandoval, Vidya Manian, Balpreet K. Dhatt, Harkamal Walia

Department of Agronomy and Horticulture: Faculty Publications

A framework combining two powerful tools of hyperspectral imaging and deep learning for the processing and classification of hyperspectral images (HSI) of rice seeds is presented. A seed-based approach that trains a three-dimensional convolutional neural network (3D-CNN) using the full seed spectral hypercube for classifying the seed images from high day and high night temperatures, both including a control group, is developed. A pixel-based seed classification approach is implemented using a deep neural network (DNN). The seed and pixel-based deep learning architectures are validated and tested using hyperspectral images from five different rice seed treatments with six different high temperature …

The Inherited Intestinal Microbiota From Myeloid-Specific Zip8ko Mice Impairs Pulmonary Host Defense Against Pneumococcal Pneumonia, Derrick R. Samuelson, Deandra R. Smith, Kelly C. Cunningham, Sabah Haq, Daniel N. Villageliú, Christi Christi Ellis, Niaz Bahar Chowdhury, Amanda E. Ramer-Tait, Jeff Price, Daren L. Knoell

Food for Health: Publications

Intestinal dysbiosis increases susceptibility to infection through the alteration of metabolic profiles, which increases morbidity. Zinc (Zn) homeostasis in mammals is tightly regulated by 24 Zn transporters. ZIP8 is unique in that it is required by myeloid cells to maintain proper host defense against bacterial pneumonia. In addition, a frequently occurring ZIP8 defective variant (SLC39A8 rs13107325) is strongly associated with inflammation-based disorders and bacterial infection. In this study, we developed a novel model to study the effects of ZIP8-mediated intestinal dysbiosis on pulmonary host defense independent of the genetic effects. Cecal microbial communities from a myeloid-specific Zip8 knockout mouse …

The Effects Of Anchor Schemes On Performance Fatigability, Neuromuscular Responses And The Perceived Sensations That Contributed To Task Termination, Robert Smith, Terry J. Housh, Jocelyn E. Arnett, John Paul V. Anders, Tyler J. Neltner, Dolores G. Ortega, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

The present study examined the effect of anchor schemes on the time to task failure (TTF), performance fatigability, neuromuscular responses, and the perceived sensations that contributed to task termination following the sustained, isometric forearm flexion tasks. Eight women completed sustained, isometric forearm flexion tasks anchored to RPE = 8 (RPEFT) and the torque (TRQFT) that corresponded to RPE = 8. The subjects performed pre-test and post-test maximal isometric contractions to quantify performance fatigability and changes in electromyographic amplitude (EMG AMP) and neuromuscular efficiency (NME). In addition, the subjects completed a post-test questionnaire (PTQ) to quantify the contributions of perceived sensations …

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 × …

Support Services And State Standardized Assessments For Homeless And High Mobility Youth: A Mixed Methods Case Study, Veronika Cummings

Department of Educational Administration: Dissertations, Theses, and Student Research

For the past decade, the number of homeless youth in the United States has drastically increased, with public schools now consistently serving over one million homeless students each year. Research spanning three decades has linked youth homelessness and poorer academic achievement. In addition to decreased academic achievement, homeless and highly mobile (HHM) youth are more likely to suffer from mental health issues, have a behavioral diagnosis, engage in high-risk behavior, be truant, abuse drugs and alcohol, and even face premature death. Because homelessness is also frequently misidentified as solely an urban problem, it often results in HHM youth being left …

Science Club, Danielle Gibson, Krista Andersen

Honors Program: Expanded Learning Clubs

Honors After School program for elementary age children to engage with scientific principles. Students performed hands-on experiments to explore various STEM fields. Each weekly lesson consisted of a different concept to be learned or explored by early elementary aged students.

Dataset Of Rna-Seq Transcriptome Of The Fetal Liver At Day 83 Of Gestation Associated With Periconceptual Maternal Nutrition In Beef Heifers, Wellison J.S. Diniz, Alison K. Ward, Kacie L. Mccathy, Cierrah J. Kassetas, Friederike Baumgaertner, Lawrence P. Reynolds, Pawel P. Borowicz, Kevin K. Sedivec, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald Scott, Joel S. Caton, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Herein, we present a dataset based on the RNA-Seq analysis of liver tissue from bovine female fetuses at day 83 of gestation. The findings were reported in the main article, “Periconceptual maternal nutrition affects fetal liver programming of energy- and lipid-related genes”[1] . These data were generated to investigate the effects of periconceptual maternal vitamin and mineral supplementation and rates of body weight gain on the transcript abundance of genes associated with fetal hepatic metabolism and function. To this end, crossbred Angus beef heifers ( n = 35) were randomly assigned to 1 of 4 treatments in a 2 ×2 …

Comparing Taste Perception Across Modalities In Healthy Adults: Liquids Versus Dissolvable Taste Strips, Ross M. Westemeyer, Angela M. Dietsch

Department of Special Education and Communication Disorders: Faculty Publications

Taste stimulation has rehabilitative value in dysphagia management, as it activates salient underlying afferent pathways to swallowing which may evoke feedforward effects on swallow biomechanics. Despite its potential beneficial effects on swallow physiology, taste stimulation’s clinical application is limited for persons unsafe to orally consume food/liquid. This study aimed to create edible, dissolvable taste strips matched to flavor profiles previously used in research assessing taste’s effects on swallowing physiology and brain activity, and to evaluate how similar their perceived intensity and hedonic, or palatability, ratings were between their liquid counterparts. Plain, sour, sweet–sour, lemon, and orange flavor profiles were custom-made …

Fusarium Species Structure In Nebraska Corn, Yuchu Ma

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Fusarium species are known to infect corn and cause significant yield losses and mycotoxin contamination worldwide. In this study, we investigated the diversity of Fusarium species infecting corn in Nebraska and their potential to produce fumonisins and trichothecenes. A total of 259 Fusarium isolates were collected from different corn tissues (ear, stalk, and root), revealing a significant association between the various Fusarium species complexes and different plant parts (p < 0.05). Fusarium incarnatum-equiseti species complex (FIESC) was the most widespread and abundant, followed by the Fusarium sambucinum (FSAMSC) and Fusarium fujikuroi species complexes (FFSC). In the subsequent analysis, we investigated the mycotoxin …

The Rna Cargo In Small Extracellular Vesicles From Chicken Eggs Is Bioactive In C57bl/6j Mice And Human Peripheral Blood Mononuclear Cells Ex Vivo, Deborah Fratantonio, Javaria Munir, Jiang Shu, Katherine Howard, Scott R. Baier, Juan Cui, Janos Zempleni

Department of Nutrition and Health Sciences: Faculty Publications

Small extracellular vesicles (sEVs) and their RNA cargo in milk are bioavailable in humans, pigs, and mice, and their dietary depletion and supplementation elicits phenotypes. Little is known about the content and biological activity of sEVs in foods of animal origin other than milk. Here we tested the hypothesis that sEVs in chicken eggs (Gallus gallus) facilitate the transfer of RNA cargo from an avian species to humans and mice, and their dietary depletion elicits phenotypes. sEVs were purified from raw egg yolk by ultracentrifugation and authenticated by transmission electron microscopy, nano-tracking device, and immunoblots. The miRNA profile …

Perspectives On Certification Of Community Health Workers: A Statewide Mixed-Methods Assessment In Nebraska, Jessica Ernmph1, Virginia Chaidez, Kate E. Trout, Kathy Karsting, Dejun Su

Department of Nutrition and Health Sciences: Faculty Publications

Objectives: While the Community Health Worker (CHW) workforce in the United States has been growing, so far only 19 states certify CHWs. This study sought to identify perspectives on CHW certification among stakeholders in Nebraska, a state that has not established official certification for CHWs yet.

Design: A concurrent triangulation mixed methods design.

Sample: Study data came from a survey of 142CHWs in Nebraska and interviews with 8 key informants employing CHWs conducted in 2019.

Methods: Logistic regression was used to identify significant factors associated with favoring CHW certification, supplemented by thematic analysis of qualitative data …

Healthcare Service Use, Help-Seeking Behaviors, And Health And Wellbeing In Attenuated Psychosis, Jasmine Ranjbari-Sisan, William D. Spaulding, Tierney K. Lorenz

Honors Program: Senior Projects (Public)

Persons with serious mental illness (SMI) receive poorer-than-standard healthcare, contributing to a 20-year reduction in the average life expectancy of persons with SMI. While extant literature describes the health disparities experienced by persons with SMI, little research examines the relationship between attenuated psychotic symptoms, healthcare service use, and help-seeking behaviors that may contribute to the disparities in this vulnerable population. This study explores the relationships between attenuated psychotic symptoms, physical health symptoms and related distress, healthcare service use, and help-seeking intentions to better understand health disparities in vulnerable and marginalized populations, such as individuals with SMI. Only a portion of …