Digital Commons Network^™

Value-Added Uses For Crude Glycerol–A Byproduct Of Biodiesel Production, Fangxia Yang, Milford Hanna, Runcang Sun

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Biodiesel is a promising alternative, and renewable, fuel. As its production increases, so does production of the principle co-product, crude glycerol. The effective utilization of crude glycerol will contribute to the viability of biodiesel. In this review, composition and quality factors of crude glycerol are discussed. The value-added utilization opportunities of crude glycerol are reviewed. The majority of crude glycerol is used as feedstock for production of other value-added chemicals, followed by animal feeds.

Field Scale Modeling To Estimate Phosphorus And Sediment Load Reductions Using A Newly Developed Graphical User Interface For Soil And Water Assessment Tool, Aaron R. Mittelstet, Erin R. Daly, Daniel E. Storm, Michael J. White, Greg A. Kloxin

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Streams throughout the North Canadian River watershed in northwest Oklahoma, USA have elevated levels of nutrients and sediment. Soil and Water Assessment Tool (SWAT) was used to identify areas that likely contributed disproportionate amounts of Phosphorus (P) and sediment to Lake Overholser, the receiving reservoir at the watershed outlet. These sites were then targeted by the Oklahoma Conservation Commission (OCC) to implement conservation practices, such as conservation tillage and pasture planting as part of a US Environmental Protection Agency Section 319(h) project. Conservation practices were implemented on 238 fields. The objective of this project was to evaluate conservation practice effectiveness …

Larval Performance And Kill Rate Of Convergent Ladybird Beetles, Hippodamia Convergens, On Black Bean Aphids, Aphis Fabae, And Pea Aphids, Acyrthosiphon Pisum, Travis M. Hinkelman, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Generalist predator guilds play a prominent role in structuring insect communities and can contribute to limiting population sizes of insect pest species. A consequence of dietary breadth, particularly in predatory insects, is the inclusion of low-quality, or even toxic, prey items in the predator’s diet. Consumption of low-quality prey items reduces growth, development, and survival of predator larvae, thereby reducing the population sizes of generalist predators. The objective of this paper was to examine the effect of a suspected low-quality aphid species, Aphis fabae (Scopoli) (Hemiptera: Aphididae), on the larval performance of an abundant North American predator, Hippodamia convergens (Guérin-Méneville) …

Evolution Of Starvation Resistance In Drosophila Melanogaster: Measurement Of Direct And Correlated Responses To Artificial Selection, Tiffany E. Schwasinger-Schmidt, Stephen D. Kachman, Lawrence G. Harshman

Lawrence G. Harshman Publications

Laboratory selection for resistance to starvation has been conducted under relatively controlled conditions to investigate direct and correlated responses to artificial selection. With regard to starvation resistance, there are three physiological routes by which the trait can evolve: resource accumulation, energy conservation and starvation tolerance. A majority of energetic compounds and macromolecules including triglycerides, trehalose and other sugars, and soluble protein increased in abundance as a result of selection. Movement was additionally investigated with selected males moving less than control males and selected females exhibiting a similar response to selection. Results obtained from this study supported two of the possible …

The Effects Of Vestibular Stimulation Rate And Magnitude Of Acceleration On Central Pattern Generation For Chest Wall Kinematics In Preterm Infants, Emily Zimmerman, Steven M. Barlow

Department of Special Education and Communication Disorders: Faculty Publications

Objective—To examine the role of vestibular inputs on respiratory and oromotor systems in healthy preterm infants.

Study Design—27 preterm infants were quasi-randomly assigned to either the VestibuGlide treatment or control groups. VestibuGlide infants were held in a developmentally supportive position, given a pacifier and received a series of vestibular stimuli, counterbalanced across rate and acceleration conditions, 15 minutes 3x/day for 10 days. The control infants were also held in a developmentally supportive position, given a pacifier for 15 minutes 3x/day for 10 days but did not receive the VestibuGlide stimulation.

Result—A multi-level regression model revealed that treatment infants increased their …

Hepatic Abc Transporters And Triglyceride Metabolism, John S. Parks, Soonkyu Chung, Gregory S. Shelness

Department of Nutrition and Health Sciences: Faculty Publications

Purpose of review—Elevated plasma triglyceride and reduced HDL concentrations are prominent features of metabolic syndrome and type 2 diabetes. Individuals with Tangier disease also have elevated plasma triglyceride concentrations and very low HDL, resulting from mutations in ATP-binding cassette transporter A1 (ABCA1), an integral membrane protein that facilitates nascent HDL particle assembly. Past studies attributed the inverse relationship between plasma HDL and triglyceride to intravascular lipid exchange and catabolic events. However, recent studies also suggest that hepatic signaling and lipid mobilization and secretion may explain how HDL affects plasma triglyceride concentrations.

Recent findings—Hepatocyte-specific ABCA1 knockout mice have markedly reduced plasma …

Insect Resistance Of A Full Sib Family Of Tetraploid Switchgrass Panicum Virgatum L. With Varying Lignin Levels, Patrick F. Dowd, Gautam Sarath, Robert B. Mitchell, Aaron J. Saathoff, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Little information is available on insect resistance mechanisms and inheritance in biomass grasses. Although reduction of lignin in biomass grasses in order to increase the efficiency of fermentation may result in increased susceptibility to insect feeding, other resistance mechanisms may be more important. Field grown leaves of two tetraploid parent (Kanlow N1, Summer) and 14 progeny switchgrass (Panicum virgatum L.) plant clones selected for a diversity of plant form and ranges in lignin levels were tested for leaf resistance to feeding by the fall armyworm (Spodoptera frugiperda J. E. Smith), a grass feeding insect pest. Although lignin generally …

Chemical Optimization Of Protein Extraction From Sweet Potato (Ipomoea Batatas) Peel, Katherine P. Maloney, Van-Den Truong, Jonathan C. Allen

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Proteins isolated from sweet potatoes (Ipomoea batatas) have been shown to possess antidiabetic, antioxidant, and antiproliferative properties. The objective of this study was to chemically optimize a process for extracting proteins from sweet potato peel. The extraction procedure involved mixing peel with saline solvent to dissolve proteins and then precipitating with CaCl₂. Quadratic and segmented models were used to determine the optimum NaCl concentration and peel to solvent ratio to maximize protein solubility while minimizing solvent usage. A segmented model was also used to optimize the concentration of CaCl2 used for precipitation. The highest yield was …

Porous Rice Powder From The Precipitation Of Gelatinized Flour Or Starch Paste With Ethanol, James Patindol, Frederick Shih, Bruce Ingber, Elaine Champagne, Stephen Boue

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Hot paste obtained by autoclaving (130ºC, 25 psi, 20–30 min) a 5–7% w/w rice flour or starch slurry was precipitated with ethanol (three extractions) to produce a dry, porous, pregelatinized powder with an average particle size of 75.0 µm (flour-derived powder) and 41.6 µm (starch-derived powder). The microstructure of the individual particles was characterized by an interconnecting lattice of irregularly shaped vesicles, and with cavities of varying size and shape. The vesicular network was relatively thinner and finer for the starch-derived products compared with the flour-derived ones. In comparison with native flour and starch, the bulk density of pregelatinized powders …

Storage Characteristics, Nutritive Value, Energy Content, And In Vivo Digestibility Of Moist, Large Rectangular Bales Of Alfalfa-Orchardgrass Hay Treated With A Propionic Acid-Based Preservative, W. K. Coblentz, K. P. Coffey, A. N. Young, M. G. Bertram

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Unstable weather, poor drying conditions, and unpredictable rainfall events often place valuable hay crops at risk. Recent research with large round bales composed of alfalfa (Medicago sativa L.) and orchard grass (Dactylis glomerata L.) has shown that these large-bale packages are particularly sensitive to spontaneous heating and dry matter (DM) losses, as well as other undesirable changes with respect to forage fiber, protein, and energy density. Various formulations of organic acids have been marketed as preservatives, normally for use on hays that are not desiccated adequately in the field to facilitate safe bale storage. Our objectives for this …

Degree Of Woody Encroachment Into Grasslands Controls Soil Carbohydrate And Amino Compound Changes During Long Term Laboratory Incubation, Courtney A. Creamer, Timothy R. Filley, Dan C. Olk, Alain Plante, Clément Peltre, Sara M. Top, Thomas W. Boutton

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Up to 50% of organic C and 80% of organic N within soil can exist as amino acids, amino sugars and carbohydrates. To determine how potential microbial accessibility and turnover of these compounds is impacted by encroachment of woody plants into grasslands, we investigated changes in evolved CO₂ during thermal analysis and in carbohydrate and amino compound chemistry after long term laboratory incubation of sandy loam grassland woodland soils from southern Texas, USA. Thermal analysis showed that incubation increased the amount of soil organic matter (SOM) released at higher temperatures and that evolved CO₂ profiles correlated with increases …

Surfactant Effects On Soil Aggregate Tensile Strength, Gary A. Lehrsch, R.E. Sojka, A.C. Koehn

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Little is known regarding a soil aggregate's tensile strength response to surfactants that may be applied to alleviate soil water repellency. Two laboratory investigations were performed to determine surfactant effects on the tensile strength of 1) Ap horizons of nine wettable, agricultural soils collected from across the continental U.S., and 2) two of the nine soils (Latahco and Rad silt loams from the Pacific Northwest) that were sampled at two depths (5 and 15 mm) after being sprinkler irrigated. Along with an untreated control, three surfactants (an alkyl polyglycoside, an ethylene oxide/propylene oxide block copolymer, and a blend of the …

Early Weaning In Northern Great Plains Beef Cattle Production Systems: Ii. Development Of Replacement Heifers Weaned At 80 Or 215 D Of Age, R.C. Waterman, T.W. Geary, J.A. Paterson, R.J. Lipsey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Studies were conducted to evaluate effects of weaning and subsequent heifer development treatments within two herds located in the Northern Great Plains, USA. Heifer calves from predominantly Angus x Hereford dams were stratified within damage and calving date (Fort Keogh Livestock and Range Research Laboratory (LARRL), Miles City, MT, USA; n=328) and randomly assigned within study and strata to one of three weaning treatments. Heifer calves from Angus and Angus x Simmental dams (n=167; Judith Gap (JG),MT,USA) were stratified within breed group by age, calving date, and AI sire. Heifer calves either remained with their dams until …

Chapter 1: The Evolution Of Switchgrass As An Energy Crop, David J. Parrish, Michael D. Casler, Andrea Monti

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This chapter discusses the prehistoric origins of switchgrass, its mid-twentieth century adoption as a crop, and late-twentieth century efforts to develop it into an energy crop. The species probably first appeared about 2 million years ago (MYA) and has continued to evolve since, producing two distinct ecotypes and widely varying ploidy levels. We build the case that all existing switchgrass lineages must be descended from plants that survived the most recent glaciation of North America and then, in just 11,000 years, re-colonized the eastern two-thirds of the continent. Moving to historic times, we discuss how switchgrass was first considered as …

Stocker Performance And Production In Mixed Tall Fescue–Bermudagrass Pastures Of The Southern Piedmont Usa, Alan J. Franzluebbers, John A. Stuedemann, Dwight H. Seman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Stocker performance and production from mixed cool- and warm-season perennial pastures are important determinants of agricultural sustainability that can be influenced by management. We evaluated the factorial combination of three sources of nutrient application (inorganic only, organic+inorganic combination, and organic only) and two forage utilization regimes [low grazing pressure (LGP) and high grazing pressure (HGP)] on steer stocking density and rate, performance and production during 7 years of pasture management {tall fescue [Lolium arundinaceum (Schreb.) Darbysh.] overseeded into existing Coastal bermudagrass [Cynodon dactylon (L.) Pers.] sod} on a Typic Kanhapludult in Georgia, USA. Nutrient source had few major …

Fum And Bik Gene Expression Contribute To Describe Fumonisin And Bikaverin Synthesis In Fusarium Verticillioides, I. Lazzaro, M. Busman, P. Battilani, R. A. E. Butchko

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fusarium verticillioides is a maize pathogen that produces toxic secondary metabolites, including fumonisins and bikaverin. The regulation of biosynthetic gene expression and the production of these metabolites are not fully understood and in this study we investigated the influence of water activity (0.955 and 0.990) on the expression of 5 genes (FUM3–FUM8–FUM13–FUM14 and BIK1) in F. verticillioides strains after 14 and 21 days incubation. Fumonisin production and biosynthetic gene expression were greatest at a_w=0.990, and the same trend was observed for bikaverin production, and BIK1 expression. FUM3 and FUM14 were the most highly expressed genes and …

Trait Convergence And Plasticity Among Native And Invasive Species In Resource-Poor Environments, Rebecca E. Drenovsky, Albina Khasanova, Jeremy J. James

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Premise of study: Functional trait comparisons provide a framework with which to assess invasion and invasion resistance. However, recent studies have found evidence for both trait convergence and divergence among coexisting dominant native and invasive species. Few studies have assessed how multiple stresses constrain trait values and plasticity, and no study has included direct measurements of nutrient conservation traits, which are critical to plants growing in low-resource environments.

Methods: We evaluated how nutrient and water stresses affect growth and allocation, water potential and gas exchange, and nitrogen (N) allocation and use traits among a suite of six codominant species from …

Modifying Crops To Increase Cell Wall Digestibility, Hans-Joachim G. Jung, Deborah A. Samac, Gautam Sarath

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Improving digestibility of roughage cell walls will improve ruminant animal performance and reduce loss of nutrients to the environment. The main digestibility impediment for dicotyledonous plants is highly lignified secondary cell walls, notably in stem secondary xylem, which become almost non-digestible. Digestibility of grasses is slowed severely by lignification of most tissues, but these cell walls remain largely digestible. Cell wall lignification creates an access barrier to potentially digestible wall material by rumen bacteria if cells have not been physically ruptured. Traditional breeding has focused on increasing total dry matter digestibility rather than cell wall digestibility, which has resulted in …

Pulling Together: Linking Unemployment Insurance And Supplemental Nutrition Assistance Program Administrative Data To Study Effects Of The Great Recession, Theresa Anderson, John A. Kirlin, Michael Wiseman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The SNAP-UI Data Linkage Project is an effort coordinated by the United States Department of Agriculture‘s Economic Research Service (ERS) to link state-level administrative data from the Supplemental Nutrition Assistance Program (SNAP) and Unemployment Insurance (UI) program to examine the concurrent and sequential patterns in use of these program before and during the Great Recession. The project focuses on calendar years 2006 through 2009 and utilizes data from seven states: California, Florida, Georgia, Illinois, Maryland, Michigan, and Texas. The project has illuminated various issues with administrative data linkage, which this paper characterizes as the ―”Three C’s" of administrative data: custody, …

Using Targeting Outcomes Of Programs As A Framework To Target Photographic Events In Nonformal Educational Programs, S. Kay Rockwell

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Targeting Outcomes of Programs (TOP) is a seven-step hierarchical programming model in which the program development and performance sides are mirror images of each other. It served as a framework to identify a simple method for targeting photographic events in nonformal education programs, indicating why, when, and how photographs would be useful to inform other evaluation strategies. In two case studies, photographs enhanced the formative story of a geoscience project being developed and tested, and contributed to the outcome narrative of a 10-year partnership project between two universities. In both cases, TOP proved to be an efficient and easy-to-use framework. …

Quail Vii: Proceedings Of The Seventh National Quail Symposium (January 9-12, 2012 : Tucson, Arizona), Clait E. Braun, Thomas V. Dailey

Galliformes Specialist Group and Affiliated Societies: Conference Proceedings

In memoriam: Walter Rosene, Jr., 1912-2005

In memoriam: John A. Crawford, 1946-2010

PLENARY

On some founding ideas of quailology and their propounders, Fred S. Guthery

One hundred years of quail management in Arizona, David E. Brown

Adaptive management and structured decision making: Is it really that easy? James (Barry) Grand

The Western Quail Management Plan, Larry Riley

Quail VII, a director’s perspective, Jonathan Gassett

BOBWHITE HABITAT MANAGEMENT

Impacts of invasive, exotic grasses on quail of southwestern rangelands: A decade of progress? William P. Kuvlesky Jr., Leonard A. Brennan, Timothy E. Fulbright, Fidel Hernández, Steven J. DeMaso, Joseph P. Sands, Robert …

Quantitative Trait Locus Analysis Of Saturated Fatty Acids In A Population Of Recombinant Inbred Lines Of Soybean, Xianzhi Wang, Guo-Liang Jiang, Marci Green, Roy A. Scott, D. L. Hyten, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Soybean [Glycine max (L.) Merr.] is an important crop which contributes approximately 58% of the world’s oilseed production. Palmitic and stearic acids are the two main saturated fatty acids in soybean oil. Different levels of saturated fatty acids are desired depending on the uses of the soybean oil. Vegetable oil low in saturated fatty acids is preferred for human consumption, while for industrial applications, soybean oil with higher levels of saturated fatty acids is more suitable. The objectives of this study were to identify quantitative trait loci (QTL) for saturated fatty acids, analyze the genetic effects of single QTL …

Ccpa Regulates Arginine Biosynthesis In Staphylococcus Aureus Through Repression Of Proline Catabolism, Austin S. Nuxoll, Steven M. Halouska, Marat R. Sadykov, Mark L. Hanke, Kenneth W. Bayles, Tammy Kielian, Robert Powers, Paul D. Fey

Department of Chemistry: Faculty Publications

Staphylococcus aureus is a leading cause of community-associated and nosocomial infections. Imperative to the success of S. aureus is the ability to adapt and utilize nutrients that are readily available. Genomic sequencing suggests that S. aureus has the genes required for synthesis of all twenty amino acids. However, in vitro experimentation demonstrates that staphylococci have multiple amino acid auxotrophies, including arginine. Although S. aureus possesses the highly conserved anabolic pathway that synthesizes arginine via glutamate, we demonstrate here that inactivation of ccpA facilitates the synthesis of arginine via the urea cycle utilizing proline as a substrate. Mutations within putA, rocD, …

A Kinematic Description Of The Temporal Characteristics Of Jaw Motion For Early Chewing: Preliminary Findings, Erin M. Wilson, Jordan R. Green, Gary Weismer

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: The purpose of this investigation was to describe age- and consistency-related changes in the temporal characteristics of chewing in typically developing children between the ages of 4 and 35 months and adults using high-resolution optically based motion capture technology.

Method: Data were collected from 60 participants (48 children, 12 adults) across 5 age ranges (beginners, 7 months, 12 months, 35 months, and adults); each age group included 12 participants. Three different food consistencies were trialed as appropriate. The data were analyzed to assess changes in chewing rate, chewing sequence duration, and estimated number of chewing cycles.

Results: The results …

Frequency Modulation And Spatiotemporal Stability Of The Scpg In Preterm Infants With Rds, Steven M. Barlow, Mimi Burch, Lalit Venkatesan, Meredith Harold, Emily Zimmerman

Department of Special Education and Communication Disorders: Faculty Publications

The nonnutritive suck (NNS) is an observable and accessible motor behavior which is often used to make inference about brain development and pre-feeding skill in preterm and term infants. The purpose of this study was to model NNS burst compression pressure dynamics in the frequency and time domain among two groups of preterm infants, including those with respiratory distress syndrome (RDS, N = 15) and 17 healthy controls. Digitized samples of NNS compression pressure waveforms recorded at a 1-week interval were collected 15 minutes prior to a scheduled feed. Regression analysis and ANOVA revealed that healthy preterm infants produced longer …

Marine Vertebrates From The Hartland Shale (Upper Cretaceous: Upper Cenomanian) In Southeastern Colorado, Usa, Matthew Nagrodski, Kenshu Shimada, Bruce A. Schumacher

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The Hartland Shale Member of the Greenhorn Limestone was deposited in the middle of the Late Cretaceous Western Interior Seaway of North America. Rock samples rich in micro-vertebrate fossils were collected from the lower part of the Hartland Shale (ca. 94.6 Ma: early Late Cenomanian) in southeastern Colorado, USA. Through acid treatment of the rock samples, 25 marine vertebrate taxa are identified including chondrichthyans, osteichthyans, and a reptile. Chondrichthyans are represented by seven species: Ptychodus anonymus, Squalicorax curvatus, Carcharias saskatchewanensis, Archaeolamna kopingensis, Cretoxyrhina mantelli, Cretomanta canadensis, and Rhinobatos incertus. Osteichthyan fishes consist of 17 taxa: Micropycnodon kansasensis, cf. Palaeobalistum …

Muc1 Mucin Stabilizes And Activates Hypoxia-Inducible Factor 1 Alpha To Regulate Metabolism In Pancreatic Cancer, Nina V. Chaika, Teklab Gebregiworgis, Michelle E. Lewallen, Vinee Purohit, Prakash Radhakrishnan, Xiang Liu, Bo Zhang, Kamiya Mehla, Roger B. Brown, Thomas Caffrey, Fang Yu, Keith R. Johnson, Robert Powers, Michael A. Hollingsworth, Pankaj K. Singh

Robert Powers Publications

Aberrant glucose metabolism is one of the hallmarks of cancer that facilitates cancer cell survival and proliferation. Here, we demonstrate that MUC1, a large, type I transmembrane protein that is overexpressed in several carcinomas including pancreatic adenocarcinoma, modulates cancer cell metabolism to facilitate growth properties of cancer cells. MUC1 occupies the promoter elements of multiple genes directly involved in glucose metabolism and regulates their expression. Furthermore, MUC1 expression enhances glycolytic activity in pancreatic cancer cells. We also demonstrate that MUC1 expression enhances in vivo glucose uptake and expression of genes involved in glucose uptake and metabolism in orthotopic implantation models …

Remote Sensing Of Sagebrush Canopy Nitrogen, Jessica J. Mitchell, Nancy F. Glenn, Temuulen T. Sankey, Dewayne R. Derryberry, Matthew J. Germino

United States Geological Survey: Staff Publications

This paper presents a combination of techniques suitable for remotely sensing foliar Nitrogen (N) in semiarid shrublands – a capability that would significantly improve our limited understanding of vegetation functionality in dryland ecosystems. The ability to estimate foliar N distributions across arid and semi-arid environments could help answer process-driven questions related to topics such as controls on canopy photosynthesis, the influence of N on carbon cycling behavior, nutrient pulse dynamics, and post-fire recovery. Our study determined that further exploration into estimating sagebrush canopy N concentrations from an airborne platform is warranted, despite remote sensing challenges inherent to open canopy systems. …

Effect Of Archer Density On Elk Pregnancy Rates And Conception Dates, Gregory A. Davidson, Bruce K. Johnson, James H. Noyes, Brian L. Dick, Michael J. Wisdom

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

Archery hunting in Oregon has increased dramatically over the past 2 decades. At the same time, spring juvenile to adult female ratios of Rocky Mountain elk (Cervus elaphus) have been declining. This has raised concern that archery seasons may be disrupting elk breeding and contributing to the decline in recruitment. Two mechanisms could contribute to reduced juvenile:female ratios: 1) reduced pregnancy rates, and 2) delayed conception dates because of human disturbance during the rut. We varied the number of archery hunters at the Starkey Experimental Forest and Range over 13 years to evaluate effects of archer density on …

Evaluation Of Monoclonal Antibody–Based Immunohistochemistry For The Detection Of European And North American Porcine Reproductive And Respiratory Syndrome Virus And A Comparison With In Situ Hybridization And Reverse Transcription Polymerase Chain Reaction, Kiwon Han, Hwi Won Seo, Yeonsu Oh, Ikjae Kang, Changhoon Park, Sang Hoon Kang, Sung-Hoon Kim, Bog-Hieu Lee, Byung Joon Kwon, Chanhee Chae

Nebraska Center for Virology: Faculty Publications

The objective of the present study was to compare the ability of 2 monoclonal antibodies (mAbs; SDOW17 and SR30) to detect types 1 and 2 Porcine reproductive and respiratory syndrome virus (PRRSV) in formalin-fixed, paraffin-embedded (FFPE) lung tissues by immunohistochemistry (IHC) and to compare the immunohistochemical results with in situ hybridization (ISH) and reverse transcription nested polymerase chain reaction (RT-nPCR) detection techniques. Lungs from 30 experimentally infected pigs (15 pigs with each genotype of PRRSV) and 20 naturally infected pigs (10 pigs with each genotype of PRRSV) with types 1 and 2 PRRSV, respectively, were used for the IHC, ISH, …