Digital Commons Network^™

Effects Of Nitrogen Availability On The Fate Of Litter-Carbon And Soil Organic Matter Decomposition, Amy Kochsiek, Johannes Knops

School of Biological Sciences: Faculty Publications

Aims: To determine whether addition of inorganic nitrogen (N) directly to maize litter (stalk and leaf) with differing tissue quality impacts litter and soil organic matter (SOM) decomposition. We tested whether N addition leads to 1) faster litter decomposition, 2) less SOM-C decomposition and 3) increased incorporation of organic-C into soil-C fractions thereby increasing C sequestration potential in maize-based systems.

Methodology: We investigated decomposition of two types of maize litter (stalk and leaf) with differing tissue quality both in the field and in a laboratory incubation experiment. In the field, litter was placed on the soil surface and at …

Holocarboxylasesynthetase Interacts Physically With Euchromatic Histone-Lysine N-Methyltransferase, Linking Histone Biotinylation With Methylation Events, Yong Li, Yousef I. Hassan, Hideaki Moriyama, Janos Zempleni

School of Biological Sciences: Faculty Publications

Holocarboxylasesynthetase (HCS) catalyzes the binding of the vitamin biotin to histonesH3 and H4, thereby creating rare histonebiotinylation marks in the epigenome. These marksco-localize with K9-methylated histone H3 (H3K9me), an abundant gene repression mark. The abundance of H3K9me marks in transcriptionally competent loci decreases when HCS is knocked down and when cells are depleted of biotin. Here we tested the hypothesis that the creation of H3K9me marks is at least partially explained by physical interactions between HCS and histone-lysine Nmethyltransferases. Using a novel in silico protocol, we predicted that HCS-interacting proteins contain a GGGG(K/R)G(I/M)R motif. Thismotif, with minor variations, is present …

Antelope Mating Strategies Facilitate Invasion Of Grasslands By A Woody Weed, Shivani Jadeja, Soumya Prasad, Suhel Quader, Kavita Isvaran

School of Biological Sciences: Faculty Publications

Intra and interspecific variation in frugivore behaviour can have important consequences for seed dispersal outcomes. However, most information comes from among-species comparisons, and within-species variation is relatively poorly understood. We examined how large intraspecific differences in the behaviour of a native disperser, blackbuck antelope Antilope cervicapra, influence dispersal of a woody invasive, Prosopis juliflora, in a grassland ecosystem. Blackbuck disperse P. juliflora seeds through their dung. In lekking blackbuck populations, males defend clustered or dispersed mating territories. Territorial male movement is restricted, and within their territories males defecate on dung-piles. In contrast, mixed-sex herds range over large areas …

Effect Of Salts On The Co-Fermentation Of Glucose And Xylose By A Genetically Engineered Strain Of Saccharomyces Cerevisiae, Elizabeth Casey, Nathan S. Mosier, Jiri Adamec, Zachary Stockdale, Nancy Ho, Miroslav Sedlak

Department of Biochemistry: Faculty Publications

Background: A challenge currently facing the cellulosic biofuel industry is the efficient fermentation of both C5 and C6 sugars in the presence of inhibitors. To overcome this challenge, microorganisms that are capable of mixed-sugar fermentation need to be further developed for increased inhibitor tolerance. However, this requires an understanding of the physiological impact of inhibitors on the microorganism. This paper investigates the effect of salts on Saccharomyces cerevisiae 424A(LNH-ST), a yeast strain capable of effectively co-fermenting glucose and xylose.

Results: In this study, we show that salts can be significant inhibitors of S. cerevisiae. All 6 pairs of anions …

Comparative Metabolic Profiling Of Haberlea Rhodopensis, Thellungiella Halophyla, And Arabidopsis Thaliana Exposed To Low Temperature, Maria Benina, Toshihiro Obata, Nikolay Mehterov, Ivan Ivanov, Veselin Petrov, Valentina Toneva, Alisdair R. Fernie, Tsanko S. Gechev

Department of Biochemistry: Faculty Publications

Haberlea rhodopensis is a resurrection species with extreme resistance to drought stress and desiccation but also with ability to withstand low temperatures and freezing stress. In order to identify biochemical strategies which contribute to Haberlea's remarkable stress tolerance, the metabolic reconfiguration of H. rhodopensis during low temperature (4^◦C) and subsequent return to optimal temperatures (21^◦C) was investigated and compared with that of the stress tolerant Thellungiella halophyla and the stress sensitive Arabidopsis thaliana. Metabolic analysis by GC-MS revealed intrinsic differences in the metabolite levels of the three species even at 21^◦C. H. rhodopensis …

Elucidating Causes Of Diporeia Decline In The Great Lakes Via Metabolomics: Physiological Responses After Exposure To Different Stressors, Suman Maity, Amber Jannasch, Jiri Adamec, James M. Watkins, Thomas Nalepa, Tomas O. Höök, Maria S. Sepúlveda

Department of Biochemistry: Faculty Publications

The benthic macroinvertebrate Diporeia spp. have been extirpated from many areas of the Laurentian Great Lakes, but the mechanisms underlying such declines are not fully understood. Diporeia declines coinciding with the invasion of exotic dreissenid mussels (zebra and quagga) have led to the hypothesis that Diporeia declines are a result of decreased food availability from increasing competition with dreissenids for diatoms. There is additional evidence that Diporeia are negatively affected when in close proximity to dreissenids, probably because of exposure to toxins present in the mussels’ pseudofeces. Diporeia are also known to be sensitive to anthropogenic contaminants (such as polychlorinated …

Nitrogen Uptake Strategies Of Edaphically Specialized Bornean Tree Species, Sabrina E. Russo, Amy Kochsiek, Jocelyn Olney, Lauren Thompson, Amy E. Miller, Sylvester Tan

School of Biological Sciences: Faculty Publications

The association of tree species with particular soil types contributes to high b diversity in forests, but the mechanisms producing such distributions are still debated. Soil nitrogen (N) often limits growth and occurs in differentially available chemical forms. In a Bornean forest where tree species composition changes dramatically along a soil gradient varying in supplies of different N-forms, we investigated whether tree species’ N-uptake and soil specialization strategies covaried. We analyzed foliar ¹⁵N natural abundance for a total of 216 tree species on clay or sandy loam (the soils at the gradient’s extremes) and conducted a ¹⁵N-tracer experiment …

A Virus-Induced Gene Silencing Method To Study Soybean Cyst Nematode Parasitism In Glycine Max, Pramod K. Kandoth, Robert Heinz, Greg Yeckel, Nathan W. Gross, Parijat S. Juvale, John Hill, Steven A. Whitham, Thomas J. Baum, Melissa G. Mitchum

School of Biological Sciences: Faculty Publications

Background: Bean pod mottle virus (BPMV) based virus-induced gene silencing (VIGS) vectors have been developed and used in soybean for the functional analysis of genes involved in disease resistance to foliar pathogens. However, BPMV-VIGS protocols for studying genes involved in disease resistance or symbiotic associations with root microbes have not been developed.

Findings: Here we describe a BPMV-VIGS protocol suitable for reverse genetic studies in soybean roots. We use this method for analyzing soybean genes involved in resistance to soybean cyst nematode (SCN). A detailed SCN screening pipeline is described.

Conclusions: The VIGS method described here provides …

Dna Sequencing Reveals Patterns Of Prey Selection In Migrating Sharp-Shinned Hawks Las Secuencias De Adn Revelan Patrones De Selección De Presas Por Individuos Migratorios De Accipiter Striatus, John Delong, Nancy Cox, Steve Cox, Zachary Hurst, Jeff Smith

School of Biological Sciences: Faculty Publications

Prey selection of migrating raptors has been documented only rarely. Here we used a genetic approach to identify avian prey of Sharp-shinned Hawks (Accipiter striatus) migrating through central New Mexico. We identified species by comparing profiles of a section of the 16S rRNA mitochondrial gene extracted from feathers of prey of known species to profiles from feathers of prey found on the feet and beaks of migrating hawks. We also quantified prey availability along the migration route with multi-year sampling by mist net at two sites near the raptor-sampling site. Sharp-shinned Hawks took most prey species in proportion to their …

Effect Of Beef Heifer Development System On Adg, Reproduction, And Feed Efficiency During First Pregnancy, Adam F. Summers, T. L. Meyer, Stetson P. Weber Weber, Rick N. Funston

Nebraska Beef Cattle Reports

This study evaluated the effect of post-weaning development system on heifer ADG, reproductive performance, and subsequent feed efficiency as a pregnant heifer. Heifers were developed on dormant pasture and grazed corn residue or dormant pasture and placed in a drylot. The following winter, a subset of pregnant heifers were placed in a Calan Broadbent individual feeding system during late gestation. Drylot-developed heifers had greater BW from pre-breeding through pregnancy diagnosis and greater overall ADG during development. However, there was no difference in reproductive performance. Pre-calving BW, ADG, and G:F tended to be greater for drylot heifers. Heifers developed on corn …

Effects Of Corn Hybrid, Plant Density, And Harvest Time On Yield And Quality Of Corn Plants, Dirk B. Burken, Jana L. Harding, Adam L. Mcgee Gee, Thomas C. Hoegemeyer, Terry Klopfenstein, Galen E. Erickson

Nebraska Beef Cattle Reports

Corn plants were collected to determine the effects of hybrid and season length, plant density, and harvest timing on grain and whole corn plant DM yield and nutritive value. Although whole corn plant DM yield decreased with delayed harvest timing, whole corn plant TDN increased linearly due to increasinggrain concentration. Increasing plant density improved yields of grain and whole corn plant DM. This experiment suggests that hybrid and season length selection, planting density, and harvest timing affect whole corn plant DM yield and quality characteristics.

Statistics Used In The Nebraska Beef Report And Their Purpose

Nebraska Beef Cattle Reports

The purpose of beef cattle and beef product research at University of Nebraska–Lincoln is to provide reference information that represents the various populations (cows, calves, heifers, feeders, carcasses, retail products, etc.) of beef production. Obviously, the researcher cannot apply treatments to every member of a population; therefore, he/she must sample the population. The use of statistics allows the researcher and readers of the Nebraska Beef Report the opportunity to evaluate separation of random (chance) occurrences and real biological effects of a treatment. Following is a brief description of the majorstatistics used in the beef report. For a more detailed description …

Use Of Patch Selection Models As A Decision Support Tool To Evaluate Mitigation Strategies Of Human–Wildlife Conflict, Sharon Baruch-Mordo, Colleen T. Webb, Stewart W. Breck, Kenneth R. Wilson

United States Department of Agriculture Wildlife Services: Staff Publications

Human-dominated landscapes offer spatially concentrated and reliable food resources that attract wildlife and lead to human–wildlife conflicts. Conflict management is often directed at humans (e.g., education) to reduce attractants, or foraging benefits to wildlife, or at wildlife (e.g., hazing) to increase foraging costs; but strategies can be expensive and ineffective. Because a key driver of conflict is the pursuit of food by wildlife, we used patch selection models, a dynamic, state-dependent modeling approach based on foraging theory, to assess how benefit reduction and cost increase resulting from conflict mitigation affect wildlife foraging decisions. We applied the patch selection models to …

Western Bean Cutworm Survival And The Development Of Economic Injury Levels And Economic Thresholds In Field Corn, Silvana L. Vieira De Paula Moraes, Thomas E. Hunt, Robert J. Wright, Gary L. Hein, Erin E. Blankenship

Department of Entomology: Faculty Publications

Western bean cutworm, Striacosta albicosta (Smith) (Lepidoptera: Noctuidae), is a native pest of dry beans (Phaseolus vulgaris L.) and corn (Zea mays L.). Historically, the western bean cutworm was distributed in the western United States, but since 1999 eastward expansion has been observed. In corn, economic impact is caused by larval ear feeding. Information on western bean cutworm biology, ecology, and economic impact is relatively limited, and the development of economic injury levels (EILs) and economic thresholds (ETs) is required for more effective management. Studies during 2008-2011, across three ecoregions of Nebraska, sought to characterize western bean cutworm …

George Eliot, Marcel Proust, And The Logic Of Desire, Kenichi Kurata

The George Eliot Review Online, ISSN: 2831-5375

The influence of George Eliot's fiction on Marcel Proust's In Search of Lost Time has often been discussed. For instance, L. A. Bisson drew attention in 1945 to 'the possibility, even the likelihood, of[ ... ] [an] immediate and sympathetic suggestion' by Eliot, citing Proust's claim in 1910 that 'II n'y a pas de litterature qui ait sur moi un pouvoir comparable a la litterature anglaise ... deux pages du Moulin sur la Floss me font pleurer.'2 Bisson goes on to suggest that three specific passages in The Mill on the Floss have their counterparts in Proust's work, the …

Factors Influencing Honors College Recruitment, Persistence, And Satisfaction At An Upper-Midwest Land Grant University, Timothy J. Nichols, Kuo-Liang Matt Chang

Journal of the National Collegiate Honors Council Online Archive

Student success and the “completion agenda” are important issues in higher education today (Complete College America). For honors programs and colleges, understanding and advancing these issues requires data-driven approaches tailored to the unique honors student population and broader institutional contexts. Honors faculty and administrators hoping to succeed in their recruitment, retention, and graduation efforts need an accurate understanding of why students decide to enroll and persist as well as their satisfaction with honors experiences. Our research data provide particular insight into the student experience at South Dakota State University (SDSU) but may also be instructive to a broader audience of …

Critical Research Needs For Successful Food Systems Adaptation To Climate Change, Michelle Miller, Molly Anderson, Charles A. Francis, Chad Kruger, Carol Barford, Jacob Park, Brent H. Mccown

Department of Agronomy and Horticulture: Faculty Publications

There is a growing sense of the fragility of agricultural production in the Global North and South and of increasing risks to food security, as scientific observations confirm significant changes in the Gulf Stream, polar ice, atmospheric CO₂, methane release, and other measures of climate change. This sense is heightened as each of us experiences extreme weather, such as the increasing frequency of droughts, floods, unseasonal temperatures, and erratic seasonality. The central research challenge before us is how global, national, regional, and local food systems may adapt to accelerating climate change stresses and uncertainties to ensure the availability, …

Effect Of Corn Bran And Steep Inclusion In Finishing Diets On Diet Digestibility, Cattle Performance, And Nutrient Mass Balance, K. M. Sayer, Crystal D. Buckner, Galen E. Erickson, Terry Klopfenstein, C.N. Macken, T.W. Loy

Department of Animal Science: Faculty Publications

One metabolism trial and 2 finishing trials were conducted to evaluate the effects of adding corn bran and steep liquor (steep) in replacement of dry-rolled corn (DRC) on diet digestibility, cattle performance, and nutrient mass balance in open feedlot pens. The metabolism trial (Exp. 1) used 8 ruminally cannulated heifers in a 4 × 4 Latin square design and the 2 finishing trials used 128 steer calves fed for 167 d (Exp. 2) and 256 yearling steers fed for 126 d (Exp. 3). Dietary treatments for all trials included a DRC-based control (CON), 30% corn bran (30/0), 30% corn bran …

The Effect Of Myostatin Genotype On Body Temperature During Extreme Temperature Events, J. T. Howard, Stephen D. Kachman, Merlyn K. Nielsen, Terry L. Mader, Matthew L. Spangler

Department of Animal Science: Faculty Publications

Extreme heat and cold events can create deleterious physiological changes in cattle as they attempt to cope. The genetic background of animals can influence their response to these events. The objective of the current study was to determine the impact of myostatin genotype (MG) on body temperature during periods of heat and cold stress. Two groups of crossbred steers and heifers of unknown pedigree and breed fraction with varying percentages of Angus, Simmental, and Piedmontese were placed in a feedlot over 2 summers and 2 winters. Before arrival, animals were genotyped for the Piedmontese-derived myostatin mutation (C313Y) to determine their …

Evaluation Of Modifications To The Neutral-Detergent-Fiber Analysis Procedure For Corn And Distillers Grains Plus Solubles, C. D. Buckner, T. J. Klopfenstein, G. E. Erickson

Department of Animal Science: Faculty Publications

Six experiments were conducted to evaluate methods for measuring NDF in corn and distillers grains plus solubles. Methods included using sodium sulfite, α-amylase (AMY), amyloglucosidase, heat and steam with an autoclave, grinding methods, and a pre–fat extraction to decrease factors that interfere with measuring NDF accurately. Using sodium sulfite and AMY resulted in decreased (P < 0.01) corn NDF values, but these decreases were different dependent on dry-rolled corn or high-moisture corn (interaction P < 0.01). Two doses of AMY (0.5 mL each) was optimum at decreasing dry-rolled corn, high-moisture corn, and steam-flaked corn NDF, but using amyloglucosidase, heat and steam with an autoclave, or both did not aid in decreasing corn NDF. Grinding corns through a 1-mm Tecator Cyclomill, compared with a Wiley mill, resulted in decreased corn NDF (P < 0.01). Using a pre–fat extraction method before the traditional NDF method resulted in decreased NDF values for distillers grains plus solubles (P < 0.01) compared with using 100 or 200 mL of NDF solution. The recommended methods for obtaining accurate NDF concentrations include using cyclo grinding and adding two 0.5 mL of AMY doses and 0.5 g of sodium sulfite for corn and pre–fat extraction for distillers grains plus solubles.

Evaluation Of A New Chemistry For Rangeland Grasshopper Control, Jeffrey Bradshaw, Karla Jenkins, Sean Doyle Whipple Whipple, Rick Patrick

Nebraska Beef Cattle Reports

A grasshopper control study was conducted to evaluate the effectiveness of a new class of systemic chemical. The new pesticide Prevathon^® (high and low levels) was compared to Coragen^®, Dimilin^®, and a nontreated check. Grasshoppers were numerically reduced the most by Coragen and Prevathon, though not significant. The highest level of Prevathon did not numerically impact beneficial insects in general. Biomass and forage quality were not significantly impacted by chemical treatment. However, forage biomass was numericallygreatest for the highest level of Prevathon. Prevathon appears to be an acceptable systemic pesticide for grasshopper control with minimal …

Block And Bridle Annual 2013

Block and Bridle Annual (1933–2021)

Dear Members, Supporters, Family, and Friends, Over the past school year, the University of Nebraska-Lincoln’s Block and Bridle Club had tremendous goal breaking accomplishments, and hosted several successful events. It is my great pleasure to recognize and thank all of the officers and members that contributed a considerable amount of time to make it a fabulous year. I would also like to thank the Animal Science Department faculty and staff for all of their support, time, and guidance throughout the year. Our advisors are a key component of our club and were crucial to the club’s success this year. They …

Environmental, Social, And Economic Footprints Of Current And Past Beef Production Systems, K.R. Stackhouse-Lawson, J. O. Reagan, B. J. Isenberg, E. J. Pollak, T. Battagliese, B. Ulhman, C. Barcan, I. Schulze, J. Silva, C. A. Rotz

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The beef industry has defined sustainability as meeting the growing demand for beef by balancing environmental responsibility, economic opportunity, and social diligence. Measuring sustainability is challenging, as the beef supply chain is one of the most complex food systems in the world. As the first and largest research project of this kind, this study represents an innovative approach toward creating a more sustainable beef product. Our objective is to establish a sustainability baseline (including environmental, economic, and social footprints) for the US beef industry by quantifying life cycle inputs and outputs for beef production over time.

Dog Burials Associated With Human Burials In The West Indies During The Early Pre-Columbian Ceramic Age (500 Bc-600 Ad), Sandrine Grouard, Sophia Perdikaris, Karyne Debue

School of Global Integrative Studies: Faculty Publications

Across the Caribbean, the widespread presence of canine remains at archaeological sites from the Saladoid period raises questions about the role of “man’s best friend.” Dog (Canis familiaris) remains have been found located in both refuse middens and burials adjacent to human graves in a number of sites in the French Antilles and Barbuda, West Indies. This paper will critically examine dog remains and discuss the varied duality of the dog’s role in the Saladoid world: from food source to lifelong companion. The importance of dogs within Amerindian sites from Saint Martin, the Guadeloupe archipelago, Martinique and Barbuda …

College Of Agricultural Sciences And Natural Resources: 20th Annual Report August 1, 2012-July 31, 2013

College of Agricultural Sciences and Natural Resources (CASNR): Annual Reports

Covers administration and staff, academic unit administrators, standing committees, ad hoc committees, departmental news and events, students, student organizations, honors, Dean's list, degrees conferred, graduate fellowships, scholarship report, alumni, Curriculum Committee actions, substitutions and waivers, online education, recruitment, retention, and placement, international engagement, teaching faculty, faculty awards and recognitions, faculty publications, grants funded, faculty appointments, undergraduate student enrollment, graduate student enrollment, and enrollment charts.

Preparing Adults To Work With Youth: An Environmental Scan Of Professional Development, Jennifer Gerdes, Tonia R. Durden, Claudia C. Mincemoyer, Kathleen Lodl

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

This feature article shares the results of a national environmental scan conducted to identify professional development programs offered for school age providers across the nation through the Cooperative Extension System. A purposeful sample comprised of representatives from state extension offices throughout the country included 135 respondents from 48 states. Results showed 139 professional development programs for school age providers were offered through the Cooperative Extension System. The majority of programs offered professional development in the areas of health, nutrition, safety, youth development, and quality afterschool environments. This article summarizes the findings of the environmental scan, including number of contact hours, …

The Role Of Forage Availability On Diet Choice And Body Condition In American Beavers (Castor Canadensis), William J. Severud, Steve K. Windels, Jerrold L. Belant, John G. Bruggink

United States National Park Service: Publications

Forage availability can affect body condition and reproduction in wildlife.Weused terrestrial and aquatic vegetation sampling, stable isotope analysis, and livetrapping to investigate the influence of estimated forage biomass on diet, body condition, and reproduction in American beavers (Castor canadensis) in the Namakan Reservoir, Voyageurs National Park, Minnesota, USA, May 2008–September 2009. Available terrestrial and emergent aquatic forage varied greatly among territories, but floating leaf aquatic forage was low in abundance in all territories. Variation in estimated biomass of available emergent and terrestrial vegetation did not explain variation in respective assimilated diets, but variation in floating leaf vegetation explained …

Effect Of Sulfur Content In Wet Or Dry Distillers Grains Fed At Several Inclusions On Cattle Growth Performance, Ruminal Parameters, And Hydrogen Sulfide, J. O. Sarturi, Galen E. Erickson, Terry Klopfenstein, Judson Vasconcelos, William A. Griffin, Kelsey Rolfe, Joshua R. Benton, Virgil R. Bremer

Department of Animal Science: Faculty Publications

Effects of S from wet or dry distillers grains with solubles (DGS) containing 0.82 or 1.16% S on animal growth performance, carcass characteristics, apparent total tract digestibility, and ruminal parameters were evaluated. In Exp. 1, crossbred beef steers (n = 120; 345 ± 34 kg BW) were individually fed ad libitum using Calan gates. Treatments were applied as a 2 × 2 × 3 + 1 factorial treatment arrangement with factors of DGS type (wet or dry), S content in DGS (0.82 or 1.16% DM basis), and DGS inclusion (20, 30, and 40%, DM basis), as well as a corn …

Characterization Of Three Chlorella Sorokiniana Strains In Anaerobic Digested Effluent From Cattle Manure, Naoko Kobayashi, Eric A. Schacht, Austin Barnes, Andrea K. Watson, Julian N. Rosenberg, Galen Erickson, George A. Oyler

Department of Animal Science: Faculty Publications

Chlorella sorokiniana CS-01, UTEX 1230 and UTEX 2714 were maintained in 10% anaerobic digester effluent (ADE) from cattle manure digestion and compared with algal cultivation in Bold’s Basal Medium (BBM). Biomass of CS-01 and UTEX 1230 in ADE produced similar or greater than 280 mg/L after 21 days in BBM, however, UTEX 2714 growth in ADE was suppressed by more than 50% demonstrating a significant species bias to synthetic compared to organic waste-based media. The highest accumulation of protein and starch was exhibited in UTEX 1230 in ADE yielding 34% and 23% ash free dry weight (AFDW), respectively, though fatty …

Effect Of Rate Of Body Weight Gain In Steers During The Stocker Phase. I. Growth, Partitioning Of Fat Among Depots, And Carcass Characteristics Of Growing-Finishing Beef Cattle, E. D. Sharman, P. A. Johnson, C. P. Mcmurphy, A. J. Garmyn, B. J. Pye, G. G. Mafi, C. L. Goad, W. A. Phillips, J. D. Starkey, C. R. Krehbiel, G. W. Horn

Department of Animal Science: Faculty Publications

Two experiments were conducted to examine the effect of growth rate to similar age or BW on fat deposition in stocker cattle grazing dormant native range (DNR) or winter wheat pasture (WP). In each experiment, fall-weaned Angus steers were randomly allotted to 1 of 4 stocker production programs: 1) control, 1.02 kg/d of a 40% CP cottonseed mealbased supplement during grazing of DNR (CON); 2) corn/soybean meal-based supplement fed at 1% of BW during grazing of DNR (CORN); 3) grazing WP at a high stocking rate to achieve a low rate of BW gain (LGWP); and 4) grazing WP at …