Digital Commons Network^™

Effects Of A Corn Root Defense Substance On Western Corn Rootworm Diabrotica Virgifera Virgifera Leconte Larvae, Zixiao Zhao

Department of Entomology: Dissertations, Theses, and Student Research

The objectives of this research were to evaluate the effects of hydroxamic acids, a group of corn root defense substances on western corn rootworm (Diabrotica virgifera virgifera LeConte) larvae and identify putative molecular mechanisms for hydroxamic acid detoxification. Corn line 428G carries a homozygous mutant bx1 gene, which makes it unable to synthesis hydroxamic acids. In contrast, H88 is the wild type parental line of 428G and is able to synthesize hydroxamic acids. Larvae were fed on 428G and H88 roots for 7 days for comparison. No significant difference was observed in growth, development, or survival rate. A RNA-Sequencing …

Aquacultured Rainbow Trout (Oncorhynchus Mykiss) Possess A Large Core Intestinal Microbiota That Is Resistant To Variation In Diet And Rearing Density, Sandi Wong, Thomas Waldrop, Steven Summerfelt, John Davidson, Frederic Barrows, P. Brett Kenney, Timothy Welch, Gregory D. Wiens, Kevin Snekvik, John F. Rawls, Christopher Good

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

As global aquaculture fish production continues to expand, an improved understanding of how environmental factors interact in fish health and production is needed. Significant advances have been made toward economical alternatives to costly fishmealbased diets, such as grain-based formulations, and toward defining the effect of rearing density on fish health and production. Little research, however, has examined the effects of fishmeal- and grain-based diets in combination with alterations in rearing density. Moreover, it is unknown whether interactions between rearing density and diet impact the composition of the fish intestinal microbiota, which might in turn impact fish health and production. We …

The Origin And Molecular Evolution Of Two Multigene Families: G-Protein Coupled Receptors And Glycoside Hydrolase Families, Seong-Il Eyun

School of Biological Sciences: Dissertations, Theses, and Student Research

Multigene family is a group of genes that arose from a common ancestor by gene duplication. Gene duplications are a major driving force of new function acquisition. Multigene family thus has a fundamental role in adaptation. To elucidate their molecular evolutionary mechanisms, I chose two multigene families: chemosensory receptors and glycoside hydrolases. I have identified complete repertoires of trace amine-associated receptors (TAARs), a member of chemosensory receptors, from 38 metazoan genomes. An ancestral-type TAAR emerged before the divergence between gnathostomes (jawed vertebrates) and sea lamprey (jawless fish). Primary amine detecting TAARs (TAAR1-4) are found to be older and have evolved …

Development Of An Enzyme-Linked Immunosorbent Assay (Elisa) For The Detection Of Pecan Residues In Processed Foods, Denise Tran

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Pecan nuts are becoming increasingly popular due to their link to health benefits. However, the presence of undeclared pecan residues in food products can pose serious health risks for pecan-allergic consumers. Currently, analytical methods for the detection of pecan allergens are limited and have not been validated for use by the food industry to assess the effectiveness of allergen control programs. Therefore, the aim of this study was to develop a sandwich-type ELISA to detect and quantify allergenic pecan residues in processed foods. Several varieties of pecan were mixed, roasted, and ground to immunize a goat, sheep, and rabbits. The …

Holocarboxylase Synthetase Represses Long Terminal Repeats Through Epigenetic Synergies Between Biotin And Methyl Donors, And Activates Immune Response By Catalyzing Biotinylation Of Heat Shock Protein 72, Jing Xue

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Holocarboxylase synthetase (HLCS) is the sole human protein biotin ligase in the human proteome. Other than its role as a coenzyme to the five carboxylases in intermediary metabolism, HLCS and biotin are involved in epigenetic gene regulation and immune response. Previously, it was shown that (1) HLCS physically interacts with histone methyl transferase EHMT-1 which creates histone H3 lysine 9 methylation (H3K9me) marks and biotinylates lysine (K) 161 in EHMT-1, thereby strenghtening the HLCS/EHMT-1 interaction; (2) biotin depletion and HLCS knockdown cause a loss of H3K9me marks leading to de-repression of long terminal repeats (LTRs) and chromosomal abnormalities; and (3) …

Center For Grassland Studies, Summer 2013, Volume 19, No. 2

Center for Grassland Studies: Newsletters

Center’s Advisory Council and Associates Tour North Central Nebraska

From the Director

Alternative Bentgrasses and Their Use

Center’s Advisory Council and Associates Tour

How Does Winter Supplementation Affect the Complete Yearling Production System?

Nebraska Grazing Lands Coalition and Nebraska Cattlemen Summer Grazing Tour Well Received

PGM Students Give Back

Nebline, July 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Integrated Pest Management in Nebraska Schools: Solving Pest Problems Effectively with Fewer Pesticides

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Assessing The Effectiveness Of Nutrition And Physical Activity Self-Assessment For Child Care (Nap Sacc) For Changes In Physical Activity Behavior, Policy, And Environment In Nebraska Family Child Care Homes, Katie K. Bolte

College of Education and Human Sciences: Dissertations, Theses, and Student Research

Nebraska is the first state to utilize NAP SACC and to modify the evidence-based program for family child care homes (FCCHs). The purpose of this study was to conduct a secondary data analysis to assess the effectiveness of a modified version of the NAP SACC approach in achieving changes in physical activity behavior, policy, and environment in Nebraska FCCHs caring for children ages 2-5 years. Results from this study will be used to improve the NAP SACC physical activity components for FCCHs and will help to inform trainers and the Nebraska Department of Health and Human Services, Nutrition and Activity …

Zein: Novel Natural Polymer For Nanoparticle- And Film-Mediated Gene Delivery, Jessica D. Taylor

Department of Agricultural and Biological Systems Engineering: Dissertations, Theses, and Student Research

Gene delivery, the introduction of DNA into cells, is applicable to gene therapy, DNA vaccination, functional genomics and diagnostics, tissue engineering, and drug-eluting medical devices. Particulates incorporating DNA are promising vehicles for gene delivery and overcome some of the barriers that hinder successful gene transfer, with the ability to protect DNA and provide for controlled, localized, and sustained release and transfection. Furthermore, innovative new gene delivery strategies that incorporate DNA particulates or complexes within films or coatings for devices and scaffolds could further provide for controlled and sustained transfection at the site of implant. Zein, a hydrophobic protein from corn, …

Identification And Characterization Of Transcription Factors Regulating Arabidopsis Hak5, Jong-Pil Hong, Yoshizumi Takeshi, Youichi Kondou, Daniel P. Schachtman, Minami Matsui, Ryoung Shin

Department of Agronomy and Horticulture: Faculty Publications

Potassium (K) is an essential macronutrient for plant growth and reproduction. HAK5, an Arabidopsis high-affinity K transporter gene, plays an important role in K uptake. Its expression is up-regulated in response to K deprivation and is rapidly down-regulated when sufficient K levels have been re-established. To identify transcription factors regulating HAK5, an Arabidopsis TF FOX (Transcription Factor Full-length cDNA Over-eXpressor) library containing approximately 800 transcription factors was used to transform lines previously transformed with a luciferase reporter gene whose expression was driven by the HAK5 promoter. When grown under sufficient K levels, 87 lines with high luciferase activity were …

Long-Term Temporal Analysis Of The Human Fecal Microbiota Revealed A Stable Core Of Dominant Bacterial Species, Ines Martınez, Catherine E. Muller, Jens Walter

Department of Food Science and Technology: Faculty Publications

Next-generation sequencing has greatly contributed to an improved ecological understanding of the human gut microbiota. Nevertheless, questions remain regarding the characteristics of this ecosystem and the ecological processes that shape it, and controversy has arisen regarding the stability of the bacterial populations and the existence of a temporal core. In this study, we have characterized the fecal microbial communities of three human individuals over a one-year period by 454 pyrosequencing of 16S rRNA tags in order to investigate the temporal characteristics of the bacterial communities. The findings revealed a temporal core of 33 to 40 species-level Operational Taxonomic Units (OTUs) …

The Epidemiology Of Human Herpesvirus-8: Transmission Of Infection To Children In Zambian Households, Kay L. Crabtree

School of Biological Sciences: Dissertations, Theses, and Student Research

Human Herpes virus-8 (HHV-8) is the known etiologic agent for several malignant pathologies, including Kaposi’s sarcoma (KS), the most common tumor in children in sub-Saharan Africa. Saliva is implicated as the culprit of transmission; however there is a paucity of information regarding transmission to young children. In this study, we investigated the hypothesis that household behaviors exposing the susceptible child to saliva increase the risk of transmission of HHV-8 to that child.

To test our hypothesis a large prospective cohort study in Lusaka, Zambia, enrolling 464 young children and their households, was followed for 48 months. Socio-demographics, health histories, feeding …

A Pilot Mixed Methods Evaluation Study Of The Effectiveness Of An After-School Cooking Club Curriculum For Middle School Students, Elisha M. Hall

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Introduction: Obesity has exponentially increased each decade, with approximately one third of the adult American population obese and another one third overweight. More startling is that these trends are rising in children as well. With economic, health, social, mental, and emotional consequences, childhood obesity has quickly risen as a significant issue to tackle. Increases in fast food consumption have been linked with overweight status, and home cooked meals are slowly becoming part of the past. The adolescent time period, in which children are slowly gaining more autonomy and conducting their activities independent from their parents, is an optimal time …

Rate Of Shattercane × Sorghum Hybridization In Situ, Jared J. Schmidt, Jeffrey F. Pedersen, Mark L. Bernards, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Cultivated sorghum [Sorghum bicolor (L.) Moench subsp. bicolor] can interbreed with a feral weedy relative shattercane [S. bicolor nothosubsp. drummondii (Steud.) de Wet ex Davidse]. Traits introduced from cultivated sorghum could contribute to the invasiveness of a shattercane population. An experiment was conducted to determine the potential for pollenmediated gene flow from grain sorghum to shattercane. Shattercane with juicy midrib (dd) was planted in soybean [Glycine max (L.) Merr.] fields during 2 yr in concentric arcs at varying distances from a 0.39 ha sorghum pollen source with dry midrib (DD). The arcs …

Characterization Of The Gut Microbiota And Colitogenic Bacterial Species In Core 1 O-Glycans Deficient Mice, Maria E. Perez-Munoz

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Mucus is one important component of the intestinal mucosal barrier, and loss of its functionality is associated with colitis in humans and mice. Mice deficient in core 1 O-glycans (TM-IEC C1galt1^-/-) display a thinner mucus layer upon mutation of the glycosyltransferase enzyme (C1GALT1) responsible for core 1 O-glycans attachment, thus constituting an experimental model for human Ulcerative Colitis (UC). Under conventional conditions, these mice experience spontaneous inflammation of the colon. However, the exact role of the microbiota in colitis development in these mice has not been systematically investigated.

Aimed to gain insights into the role of …

Methods For Quantifying T Cell Receptor Binding Affinities And Thermodynamics, Kurt H. Piepenbrink, Brian E. Gloor, Kathryn M. Armstrong, Brian M. Baker

Food for Health: Publications

αβ T cell receptors (TCRs) recognize peptide antigens bound and presented by class I or class II major histocompatibility complex (MHC) proteins. Recognition of a peptide/MHC complex is required for initiation and propagation of a cellular immune response, as well as the development and maintenance of the T cell repertoire. Here we discuss methods to quantify the affinities and thermodynamics of interactions between soluble ectodomains of TCRs and their peptide/MHC ligands, focusing on titration calorimetry, surface plasmon resonance, and fluorescence anisotropy. As TCRs typically bind ligand with weak-to-moderate affinities, we focus the discussion on means to enhance the accuracy and …

Using High-Fiber Diets To Limit Energy Intake In Developing Gilts: Effects On Puberty, Reproduction, Culling Rates, Lifetime Productivity, And Progeny Health And Growth, Phillip S. Miller, Rodger Johnson, Justin Bundy, Daniel Ciobanu

Department of Animal Science: Faculty Publications

A total of 182 gilts were used to evaluate the effects of limiting energy intake during development compared to traditional corn-soybean meal based diets using the addition of soybean hulls to reduce caloric intake with ad libitum access to feed. The gilt developmental period consisted of 110 days, from 120 to 230 days of age. Data collected during development included body weight, backfat, longissimus muscle area, and feed intake, every 2 weeks. Estrus detection was conducted on a daily basis until breeding. Females were maintained in the breeding groups for 4 parities (785 days of age), at which time data …

Nebline, June 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Smart Family Finances

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

The Impact Of Environmental Regulation On The Structure Of The Us Hog Industry, Gibson Nene, Azzeddine M. Azzam, Karina Schoengold

Department of Agricultural Economics: Faculty Publications

Abstract: This paper develops a comparative statics model of long-run industry equilibrium in the presence of size-based environmental regulation stringency and applies the model to the United States hog industry. The economic model shows that when size-based environmental stringency is also size-biased, large farms downsize, expand, or do neither depending on how environmental stringency shifts their marginal production cost relative to their average cost. Empirical testing using data from the top-ten hog producing states suggests that environmental regulation stringency has limited impact on small farms and leads to a reduction in the number of large farms. We cannot reject positive …

Diets And Food Selection Of Female Mallards And Blue-Winged Teal During Spring Migration, Paul R. Tidwell, Elisabeth B. Webb, Mark P. Vrtiska, Andrew A. Bishop

Nebraska Game and Parks Commission: Staff Research Publications

Waterfowl nutritional requirements and food availability at migration stopover habitats may differ from those at nesting or wintering areas. Although there is little information on factors that influence waterfowl diets and food selection during migration, we hypothesized that bird age and wetland density in the surrounding landscape would influence food selection. Thus, the objective of this study was to quantify mallard Anas platyrhynchos and blue-winged teal Anas discors diets during migration and evaluate effects of age and wetland density on waterfowl food selection. We collected 30 mallards and 29 blue-winged teal with food items present in esophagi from wetlands in …

Utilization Of Wet Corn Distillers Grain For The Production Of Cellulase Enzymes And Value Added Products, Hunter R. Flodman

Department of Chemical and Biomolecular Engineering: Dissertations, Theses, and Student Research

Distillers grain, a byproduct from the dry grind corn ethanol industry, is an industrially available, abundant, preprocessed material comprised primarily of cellulose, hemicellulose, protein, oil, and minerals. This research investigated the utilization of distillers grain as a substrate for solid-state fermentation (SSF). SSF is the growth of microorganisms on a moist, solid substrate in the absence of free water. The substrate acts as both the nutrient source and as a solid support for the microorganisms, typically filamentous fungi. SSF has the unique ability to convert agricultural byproducts into valuable bioproducts such as enzymes and organic acids without hydrolyzing polysaccharides to …

Decomposition And Nutrient Release Of Different Cover Crops In Organic Farm Systems, Jianru Shi

School of Natural Resources: Dissertations, Theses, and Student Research

Cover crops act as green manure adding organic matter to agricultural-soils. For legume green manures to be an effective nitrogen (N) source for organic farming systems, their N release must be in synchrony with crop N demand. The objectives of this study were 1) determine the decomposition rates of three common cover crops (white clover, (Trifolium repens,L ) red clover (Trifolium pratense L.) and soybean (Glycine max L) in order to determine when most N was released and its synchrony with subsequent corn crop uptake; 2) we focused on the effect of cover crops on soil …

Providing More—And Better—Food By 2050, John W. Finley

Agricultural Research Magazine

The world in 2050 is predicted to have 9 billion people, who will differ in almost every way but one: They will all need to eat on a daily basis.

Their nutritional needs will be similar, too. Nutrition means eating, eating means food, and food means agriculture. Many people speak of the increased food needed for 9 billion, but in reality, the increased need is for nutrition. Thus, the world of 2050 will force a melding of two disciplines that have diverged in recent years—agriculture and nutrition.

We need to change our focus. We need sustainable nutrition as much as …

May/June 2013- Locations Featured In This Magazine Issue

Agricultural Research Magazine

The Agricultural Research Service has about 100 labs all over the country.

Locations Featured in This Magazine Issue

Albany, California 9 research units ■ 230 employees

Pullman, Washington 6 research units ■ 136 employees

Small Grains and Potato Germplasm Research Unit, Aberdeen, Idaho 1 research unit ■ 57 employees

U.S. Sheep Experiment Station, Dubois, Idaho 1 research unit ■ 10 employees

Roman L. Hruska U.S. Meat Animal Research Center, Clay Center, Nebraska 6 research units ■ 117 employees

Grand Forks Human Nutrition Research Center, Grand Forks, North Dakota 2 research units ■ 51 employees

Children’s Nutrition Research Center, Houston, Texas …

Agricultural Research Magazine, May-June 2013

Agricultural Research Magazine

Table of Contents

4 Olives! Scientists Take a New Look at an Ancient Crop

8 Getting Hooked on Farmed Salmon: A Good Source of Omega-3s

10 Nematode-Filled Capsules Tested Against Corn Rootworms

12 Viral Infection in Sheep Linked to Gene

14 Cited for More Than 60 Years of Flavor Research

17 Dogs, Pine Trees, and Carbs: Studies Lead to New Pet- Food Ingredient

18 Nourishing America’s Preemies: Scientists Confront the Challenges of IV Feeding

20 Mapping the Way to Even Healthier Rice

22 New Bioenergy Yeast Is an Overachiever

23 Locations Featured in This Magazine Issue

Mapping The Way To Even Healthier Rice, Shannon Pinson, Jan Suszkiw

Agricultural Research Magazine

With the aid of high-tech tools, Agricultural Research Service and collaborating scientists are closing in on the genes in rice that regulate the uptake and storage of important minerals—a pursuit that could bolster the nutritional value of this cereal grain crop as a staple food for roughly half the world’s population.

Ultimately, the team envisions biofortifying rice using traditional plant breeding methods to develop new varieties whose kernels (grain) boast exceptionally high concentrations of essential minerals, including zinc, iron, and calcium.

Rice is a mainstay of the human diet for good reason. It is a rich source of energy, free …

Getting Hooked On Farmed Salmon: A Good Source Of Omega-3s, Susan Raatz, Matthew Picklo, William Wolters, Rosalie Marion Bliss

Agricultural Research Magazine

Nearly 85 percent of seafood enjoyed by U.S. consumers is imported, and almost half of that is farm raised through a process called “aquaculture,” according to the National Aquaculture Association, based in Pine Bluff, Arkansas. In 2011, the United States imported $16.6 billion of seafood and exported $5.4 billion, a difference of $11.2 billion.

As the world’s population grows and demand for seafood increases, many seafood species are overfished or fully exploited. That means the world’s oceans cannot turn out more fish than are currently being produced. As the ceiling is being reached on seafood availability from the wild, U.S. …

Larval Performance And Kill Rate Of Convergent Ladybird Beetles, Hippodamia Convergens, On Black Bean Aphids, Aphis Fabae, And Pea Aphids, Acyrthosiphon Pisum, Travis M. Hinkelman, Brigitte Tenhumberg

School of Biological Sciences: Faculty Publications

Generalist predator guilds play a prominent role in structuring insect communities and can contribute to limiting population sizes of insect pest species. A consequence of dietary breadth, particularly in predatory insects, is the inclusion of low-quality, or even toxic, prey items in the predator’s diet. Consumption of low-quality prey items reduces growth, development, and survival of predator larvae, thereby reducing the population sizes of generalist predators. The objective of this paper was to examine the effect of a suspected low-quality aphid species, Aphis fabae (Scopoli) (Hemiptera: Aphididae), on the larval performance of an abundant North American predator, Hippodamia convergens (Guérin-Méneville) …

Nebline, May 2013

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Moving Toward a Healthier You

Farm Views

Urban Agriculture

Food & Fitness

Home & Family Living

Horticulture

Environmental Focus

4-H & Youth

Community Focus

Extension Calendar

Nutrition Education Program

Family and Community Education (FCE) Clubs

and other extension news and events

Dogs, Pine Trees, And Carbs, Neil P.J. Price, Marcia Wood

Agricultural Research Magazine

Carbohydrates from a perhaps surprising source—pine trees—may have beneficial effects on dogs’ digestive-system health, according to collaborative studies by Agricultural Research Service, university, and corporate scientists.

The carbs, predominantly a group known as “GGMOs”—short for galactoglucomannan oligosaccharides—are a key ingredient in Previda, an all-natural dietary ingredient marketed to makers of pet food, aquaculture feed, and other animal-nutrition products.

ARS chemist Neil P.J. Price began his studies of these fiber-rich carbs in 2007, working under the auspices of a cooperative agreement with Temple-Inland, a Texas-based producer of lumber, fiberboard, and other wood products made from loblolly, longleaf, and other kinds of …