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Nebline, September 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: 4-H Youth “Let the Good Times Grow” at Super Fair

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Evaluating Sub-Lethal Effects Of Orchard-Applied Pyrethroids Using Video-Tracking Software To Quantify Honey Bee Behaviors, Erin M. Ingram, Julie Augustin, Marion D. Ellis, Blair Siegfried

Department of Entomology: Faculty Publications

Managed honey bee, Apis mellifera L., colonies are contracted to pollinate fruit and nut orchards improving crop quality and yield. Colonies placed in orchards are potentially exposed to pyrethroid insecticides used for broad-spectrum pest control. Pyrethroids have been reported to pose minimal risk to bees due to their low application rates in the field and putative repellent properties. This repellency is believed to alter foraging behavior with the benefit of preventing bees from encountering a lethal dose in the field. However, sub-lethal exposure to pyrethroids may adversely impact bee behavior potentially resulting in social dysfunction or disruption of foraging.

This …

Electromyographic Responses From The Vastus Medialis During Isometric Muscle Actions, C. M. Smith, Terry J. Housh, Trent J. Herda, Jorge M Zuniga, Clayton L. Camic, Haley Bergstrom, D.B. Smith, John R. Weir, Joel T. Cramer, E.C. Hill, Kristen C. Cochrane, N. D. M. Jenkins, R. Schmidt, G. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

This study examined the electromyographic (EMG) responses from the vastus medialis (VM) for electrodes placed over and away from the innervation zone (IZ) during a maximal voluntary isometric contraction (MVIC) and sustained, submaximal isometric muscle action. A linear electrode array was placed on the VM to identify the IZ and muscle fiber pennation angle during an MVIC and sustained isometric muscle action at 50 % MVIC. EMG amplitude and frequency parameters were determined from 7 bipolar channels of the electrode array, including over the IZ, as well as 10 mm, 20 mm and 30 mm proximal and distal to the …

Chlorophyll Synthase Under Epigenetic Surveillance Is Critical For Vitamin E Synthesis, And Altered Expression Affects Tocopherol Levels In Arabidopsis, Chunyu Zhang, Wei Zhang, Guodong Ren, Denli Li, Rebecca E. Cahoon, Ming Chen, Yongming Zhou, Bin Yu, Edgar B. Cahoon

Center for Plant Science Innovation: Faculty Publications

Chlorophyll synthase catalyzes the final step in chlorophyll biosynthesis: the esterification of chlorophyllide with either geranylgeranyl diphosphate or phytyl diphosphate (PDP). Recent studies have pointed to the involvement of chlorophyll-linked reduction of geranylgeranyl by geranylgeranyl reductase as a major pathway for the synthesis of the PDP precursor of tocopherols. This indirect pathway of PDP synthesis suggests a key role of chlorophyll synthase in tocopherol production to generate the geranylgeranyl-chlorophyll substrate for geranylgeranyl reductase. In this study, contributions of chlorophyll synthase to tocopherol formation in Arabidopsis (Arabidopsis thaliana) were explored by disrupting and altering expression of the corresponding gene …

Nebline, August 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Nutrition Education Program Reaches 15-Year-Milestone Teaching Nutrition in Lincoln Schools

Food & Health

Family Living

Farm & Acreage

Home & Family Living

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Special pullout section: Lancaster County Super Fair 2015 Schedule & Map

A Dual Function Of White Coloration In A Nocturnal Spider Dolomedes Raptor (Araneae: Pisauridae), Tai-Shen Lin, Shichang Zhang, Chen-Pan Liao, Eileen A. Hebets, I-Min Tso

Eileen Hebets Publications

Nocturnal animals frequently possess seemingly conspicuous color patterns that can function in a variety of ways (e.g. prey attraction, camouflage, predator avoidance, etc.). The use of color patterns in intraspecific signaling, especially reproductive activities, in nocturnal animals has received relatively little attention. This study tested for a dual function of color in the nocturnal fishing spider, Dolomedes raptor (Araneae: Pisauridae), whose males develop dimorphic white stripes at sexual maturation. We tested for a role in foraging as well as mate assessment. First, quantifications of the natural variation of male stripes indicated a correlation between stripe area and male body size …

Beef Cattle Management Systems For Estrus Synchronization And Heifer Development, Hazy Rae Nielson

Department of Animal Science: Dissertations, Theses, and Student Research

Four experiments were conducted to evaluate estrus synchronization and heifer development systems. In the first experiment delaying AI 19 h following GnRH administration in a hybrid estrus detection and time AI protocol was evaluated. Final pregnancy rate was not different among heifers detected in estrus, AI at the time GnRH administration, or delayed AI. The second study compared the effect of melengestrol acetate (MGA) -PG and 14-d controlled internal drug release (CIDR) -PG estrus synchronization protocols on estrus response and pregnancy rates of 311 d old heifers. Final pregnancy rate was similar between CIDR and MGA treatment groups. The objective …

Dietary Strategies For Mitigation Of Methane Production By Growing And Finishing Cattle, Anna C. Pesta

Department of Animal Science: Dissertations, Theses, and Student Research

Interest in reducing methane (CH₄) emissions by cattle has increased recently. Loss of feed energy as CH₄ represents both an environmental concern and an energetic loss to the animal. Manipulation of dietary composition has proven to be an effective mitigation strategy. Experiments were conducted to evaluate the effect of several dietary characteristics on CH₄ production in growing and finishing cattle, and to develop a novel, non-invasive method for quantifying emissions from animals in a production setting. Short-term gaseous emissions of CH₄ and carbon dioxide (CO₂) were measured and the CH₄:CO_{2 …}

Effects Of Age, Sex, And Body Position On Orofacial Muscle Tone In Healthy Adults, Angela M. Dietsch, Heather M. Clark, Jessica N. Steiner, Nancy Pearl Solomon

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: Quantification of tissue stiffness may facilitate identification of abnormalities in orofacial muscle tone and thus contribute to differential diagnosis of dysarthria. Tissue stiffness is affected by muscle tone as well as age-related changes in muscle and connective tissue.

Method: The Myoton-3 measured tissue stiffness in 40 healthy adults, including equal numbers of men and women in each of two age groups: 18–40 years and 60+ years. Data were collected from relaxed muscles at the masseter, cheek, and lateral tongue surfaces in two positions: reclined on the side and seated with head tilted.

Results: Tissue stiffness differed …

Investigation Of Reduced Agent And Area Treatments For Aphis Glycines Management And Its Effects On Key Predators, Jenny S. Enchayan

Department of Entomology: Dissertations, Theses, and Student Research

Soybeans are an abundant and valued agricultural crop in Nebraska and other parts of the world. Significant soybean aphid, Aphis glycines Matsumura, mortality has been observed in research plots adjacent to soybeans treated with chlorpyrifos in late July through August. The objectives of the study were to determine the efficacy of the chlorpyrifos vapor effect on A. glycines in adjacent untreated areas and assess the impact of the vapor effect on key biological control agents. Observation of A. glycines populations and sweep net samples for other arthropod species, including Oruis insidiosus, began weekly in mid to late July. When …

Ecology And Morphology Of The Late Miocene Musk Deer, Longirostromeryx Wellsi (Artiodactyla: Moschidae: Blastomerycinae), Katheryn Y. C. Chen

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Longirostromeryx wellsi, one of the latest surviving members of the extinct clade Blastomerycinae (Artiodactyla: Moschidae), possesses highly derived craniodental morphology that deviates from typical musk deer form. Previous work suggests that the unique anatomy of L. wellsi represents adaptations for occupying open savannas. To test this hypothesis I conduct principal components analysis on five postcranial bones of L. wellsi, comparing them to that of several extant ruminant artiodactyls, which are divided among seven habitat categories. These elements are also compared with the postcrania of other blastomerycines. These analyses indicate that L. wellsi anatomy is most similar to that of other …

The Effectiveness Of Educational Displays Related To Foodborne Illness In Infants, Krista M. Godfrey

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Infants under one year of age are at high risk and have the highest incidence of foodborne illnesses. The majority of infants receive infant formula before the age of one. Powdered infant formula (PIF) is not sterile, and if not prepared or handled appropriately, may cause foodborne illness. Researchers suggest that the majority of mothers do not receive information regarding safe handling of infant formula, and many do not use recommended procedures when preparing formula. The study objective was to develop and evaluate four display concepts to educate parents on the FDA/CDC recommendation to dispose PIF after two hours at …

Ability Of The Gut Microbiota To Produce Pufa-Derived Bacterial Metabolites: Proof Of Concept In Germ-Free Versus Conventionalized Mice, Céline Druart, Laure B. Bindels, Robert Schmaltz, Audrey M. Neyrinck, Patrice D. Cani, Jens Walter, Amanda E. Ramer-Tait, Nathalie M. Delzenne

Department of Food Science and Technology: Faculty Publications

Scope—The gut microbiota is able to modulate host physiology through the production of bioactive metabolites. Our recent studies suggest that changes in gut microbiota composition upon prebiotics supplementation alter tissue levels of PUFA-derived metabolites in mice. However, in vivo evidence that gut microbes produces PUFA-derived metabolites is lacking. This study aimed to decipher the contribution of gut microbes versus that of the host in PUFA-derived metabolite production.

Methods and results—To achieve this goal, we compared the proportion of PUFA-derived metabolites and the expression of fatty acid desaturases in germ-free (GF) and conventionalized (CONV) mice fed either a low …

4-H 357 Guide For Evaluating Livestock Showmanship Contests : Beef, Sheep, Swine, Doyle R. Wolverton

Nebraska 4-H Clubs: Historical Materials and Publications

Nebraska Cooperative Extension 4-H 357: Guide for Evaluating Livestock Showmanship Contests. This guide provides an outline of various desirable showmanship practices and the relative importance of each.

Nitrogen And Water Effects On Canopy Sensor Measurements For Site-Specific Management Of Crops, Nicholas C. Ward

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Water and nitrogen (N) are undoubtedly the two largest agricultural inputs globally. Coupled with advances in site-specific management technology their integration into production agriculture will allow for the most efficient use these crop input resources. Active canopy sensors offer the ability to measure biophysical plant traits rapidly and make assessments about plant status. Specifically, optical sensor measurements of light reflectance assess plant N status allowing for in-season and on-the-go N recommendations and applications; while infrared thermometers (IRT) measurement of canopy temperature can be used a tool for irrigation management. To evaluate how these technologies work among different plant stress environments …

Agave Chewing And Dental Wear: Evidence From Quids, Emily E. Hammerl, Melissa A. Baier, Karl Reinhard

Karl Reinhard Publications

Agave quid chewing is examined as a potential contributing behavior to hunter-gatherer dental wear. It has previously been hypothesized that the contribution of Agave quid chewing to dental wear would be observed in communities wherever phytolith-rich desert succulents were part of subsistence. Previous analysis of coprolites from a prehistoric agricultural site, La Cueva de los Muertos Chiquitos in Durango, Mexico, showed that Agave was a consistent part of a diverse diet. Therefore, quids recovered at this site ought to be useful materials to test the hypothesis that dental wear was related to desert succulent consumption. The quids recovered from the …

Competing Infant Feeding Information In Mothers’ Networks: Advice That Supports V. Undermines Clinical Recommendations, Sato Ashida, Freda B. Lynn, Natalie A. Williams

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To identify the social contextual factors, specifically the presence of information that supports v. undermines clinical recommendations, associated with infant feeding behaviours among mothers in low-income areas.

Design: Cross-sectional survey evaluating social support networks and social relationships involved in providing care to the infant along with feeding beliefs and practices.

Setting: Out-patient paediatric and government-funded (Women, Infants, and Children) clinics in an urban, low-income area of the south-eastern USA.

Subjects: Eighty-one low-income mothers of infants between 0 and 12 months old.

Results: Most mothers reported receiving both supportive and undermining advice. The presence of …

Competing Infant Feeding Information In Mothers’ Networks: Advice That Supports V. Undermines Clinical Recommendations, Sato Ashida, Freda B. Lynn, Natalie A. Williams, Ellen J. Schafer

Department of Child, Youth, and Family Studies: Faculty Publications

Objective: To identify the social contextual factors, specifically the presence of information that supports v. undermines clinical recommendations, associated with infant feeding behaviours among mothers in low-income areas.

Design: Cross-sectional survey evaluating social support networks and social relationships involved in providing care to the infant along with feeding beliefs and practices.

Setting: Out-patient paediatric and government-funded (Women, Infants, and Children) clinics in an urban, low-income area of the south-eastern USA.

Subjects: Eighty-one low-income mothers of infants between 0 and 12 months old.

Results: Most mothers reported receiving both supportive and undermining advice. The presence …

Biodegradable Medical Device Having An Adjustable Degradation Rate And Methods Of Making The Same, Yuebin Guo, Michael Sealy, Meisam Salahshoor Pirsoltan

Department of Mechanical and Materials Engineering: Faculty Publications

Disclosed herein are biodegradable medical devices comprising biodegradable material (e.g., magnesium-calcium alloys) having an adjustable rate of degradation that can be used in various applications, including, but not limited to, drug delivery applications, cardiovascular applications, and orthopedic applications to make biodegradable and biocompatible devices. Also disclosed herein are methods of making biodegradable medical devices comprising biodegradable materials by using, for instance, hybrid dry cutting/hydrostatic burnishing.

Influence Of Native And Processed Cereal Grain Fibers On Gut Health, Junyi Yang

Department of Food Science and Technology: Dissertations, Theses, and Student Research

Cereal fibers that can be metabolized by gut microbiota have been shown to promote the growth of beneficial bacteria in the gut. Increased consumption of cereal fibers may improve host / gut microbiota interactions in obesity and other metabolic diseases by normalizing gut dysbiosis. The present dissertation describes four research projects to assess the impact of cereal dietary fibers on gut microbiota and host metabolism. In the first study, we determined the treatment temperatures for production of soluble, non-digestible, feruloylated oligo- and polysaccharides (FOPS) from maize bran and wheat bran, and determined the fermentation properties of partially purified FOPS from …

Lh-Independent Testosterone Secretion Is Mediated By The Interaction Between Gnrh2 And Its Receptor Within Porcine Testes, Amy T. Desaulniers, Rebecca A. Cederberg, Ginger A. Mills, J. Joe Ford, Clay A. Lents, Brett R. White

Department of Animal Science: Faculty Publications

Unlike classic gonadotropin-releasing hormone 1 (GNRH1), the second mammalian isoform (GNRH2) is an ineffective stimulant of gonadotropin release. Species that produce GNRH2 may not maintain a functional GNRH2 receptor (GNRHR2) due to coding errors. A full-length GNRHR2 gene has been identified in swine, but its role in reproduction requires further elucidation. Our objective was to examine the role of GNRH2 and GNRHR2 in testicular function of boars. We discovered that GNRH2 levels were higher in the testis than in the anterior pituitary gland or hypothalamus, corresponding to greater GNRHR2 abundance in the testis versus the anterior pituitary gland. Moreover, GNRH2 …

Nebline, July 2015

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Reduce Bullying and Cyberbullying Through Social and Emotional Learning

Food & Health

Family Living

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Extension Calendar

and other extension news and events

Understanding Childhood Maltreatment: Literature Review And Practical Applications For Educators, Sarah E. Wright

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The purpose of this thesis is to conduct a critical and descriptive review of the research related to children who experience trauma due to maltreatment—whether because of physical, sexual, emotional, or psychological abuse and neglect—their effects on children, and the potential impact in school and how educational personnel can support the needs of these students. An overview of child maltreatment and review of the literature related to children who have experienced maltreatment was provided including: (a) type of maltreatment, (b) prevalence of maltreatment, (c) effects of maltreatment, (d) treatment approaches, and (f) relevance for educators. The methods and results for …

Predictors Of Preschool Children's Body Mass Index: Breastfeeding Duration, Child Eating Behaviors, And Parental Feeding Practices, Amy J. Encinger

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The current study examined how breastfeeding duration, child eating behaviors (Satiety and Food Responsiveness), and parental feeding practices (Environment and Parental Control over Food) are related to body mass index (BMI) among preschool-aged children. Participants were 224 primary caregivers (M age = 30.36; 90% mothers) of preschool-aged children (M age = 4.22 years, 55% male) from three Midwestern preschools serving low-income children. Mediated multiple regression analyses examined the effects of the various child eating behaviors and parental feeding practices as mediators of the relation between breastfeeding duration and child BMI. Three main findings emerged. There was a significant …

Discovery Neighborhood Myplate Macy: A Pilot Study, Amanda M. Robine

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Background: Obesity prevalence in United States children ages 2-19 years has been consistently high since 2003.1 These children are at a greater risk for many chronic disease. Educating children on how to make healthful food choices, and using fun characters in a classroom setting may be one step to reducing the obesity rate.

Objective: Increase knowledge about MyPlate and heathy eating in children in kindergarten through fifth grade.

Design: After the development of a nutrition lesson, an expert panel reviewed and suggested changes. Once changes were made the lesson was delivered to second and third graders at a summer camp. …

Effects Of Antiretroviral Therapy On Kaposi’S Sarcoma–Associated Herpesvirus (Kshv) Transmission Among Hiv-Infected Zambian Children, Landon Olp, Veenu Minhas, Clement Gondwe, Chipepo Kankasa, Janet M. Wojcicki, Charles Mitchell, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Background: The risk of Kaposi’s sarcoma–associated herpesvirus (KSHV) acquisition among children is increased by HIV infection. Antiretroviral therapy (ART) was recently made widely available to HIV-infected children in Zambia. However, the impact of early ART on KSHV transmission to HIV-infected children is unknown.

Methods: We enrolled and followed a cohort of 287 HIV-exposed, KSHV-negative children under 12 months of age from Lusaka, Zambia, to identify KSHV seroconversion events. Potential factors associated with KSHV infection—with an emphasis on HIV, ART, and immunological measures—were assessed through structured questionnaires and blood analyses. Incidence rate, Kaplan- Meier, and multivariable Cox regression models were used …

Perceived Discrimination And Markers Of Cardiovascular Risk Among Low-Income African American Youth, Bridget J. Goosby, Sarah Malone, Elizabeth A. Richardson, Jacob Cheadle, Deadric T. Williams

Department of Sociology: Faculty Publications

Objectives: Our study examines the relationship between perceived discrimination and levels of C-reactive protein and blood pressure in low-income youth ages 10215 years old.

Methods: Data were collected from 10 to 15 year old focal children and their mothers. Face-to-face interviews were implemented to collect data on stressors including experiences of everyday discrimination from youth. High sensitivity CRP in dried blood spot samples and diastolic and systolic blood pressure were also collected at the time of the interview.

Results: Perceived discrimination among youth was significantly associated with higher levels of CRP, systolic, and diastolic blood pressure. CRP, systolic, and diastolic …

Biochemical And Proteomic Profiling Of Maize Endosperm Texture And Protein Quality, Kyla J. Morton

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The research described herein, focuses on the biochemical and proteomic analysis of the maize endosperm and what influences kernel texture. Quality Protein Maize (QPM) is a hard endosperm version of the high-lysine opaque2 (o2) mutant but the genes involved in modifying the soft o2 endosperm are unknown. Pyrophosphate (PPi)-dependent fructose 6-phosphate 1-phosphotransferase (PFP) catalyzes the ATP-independent conversion of fructose 6-phosphate to fructose 1, 6-bisphosphate in glycolysis. We found a large increase in transcript and protein levels of the alpha regulatory subunit of PFP (PFPα) in QPM endosperm. In vitro enzyme assays show a significant increase in forward PFP …

Mexican Red Kneed Tarantula (Brachypelma Smithi) Care Manual, Jamie Sincage, Trace Hardin

Department of Entomology: Distance Master of Science Projects

Caring for any animal is more of an art than a science. What works for one keeper may not necessarily work for another. This may be based on unnoticed nuances, local climate or weather patterns, the personalities of the individual animals, or other minute details. This is a basic husbandry and breeding guide to the Mexican red knee tarantula (Brachypelma smithi). Any more specifics will have to be discovered personally or discussed with colleagues. Where many vertebrates have been kept in captivity for centuries a lot of invertebrates are relatively new to the husbandry world. Even though humans …

Combinatorial Effects Of Fatty Acid Elongase Enzymes On Nervonic Acid Production In Camelina Sativa, Dongxin Huai, Yuanyuan Zhang, Chunyu Zhang, Edgar B. Cahoon, Yongming Zhou

Center for Plant Science Innovation: Faculty Publications

Very long chain fatty acids (VLCFAs) with chain lengths of 20 carbons and longer provide feedstocks for various applications; therefore, improvement of VLCFA contents in seeds has become an important goal for oilseed enhancement. VLCFA biosynthesis is controlled by a multi-enzyme protein complex referred to as fatty acid elongase, which is composed of β-ketoacyl-CoA synthase (KCS), β-ketoacyl-CoA reductase (KCR), β-hydroxyacyl-CoA dehydratase (HCD) and enoyl reductase (ECR). KCS has been identified as the ratelimiting enzyme, but little is known about the involvement of other three enzymes in VLCFA production. Here, the combinatorial effects of fatty acid elongase enzymes on VLCFA production …