Digital Commons Network^™

Effect Of Feeding Three Lysine To Energy Diets On Growth, Body Composition And Age At Puberty In Replacement Gilts, J.A. Calderón Díaz, J. L. Vallet, R.D. Boyd, Clay A. Lents, T.J. Prince, A.E. Dedecker, C.E. Phillips, G. Foxcroft, Kenneth J. Stalder

Roman L. Hruska U.S. Meat Animal Research Center: Reports

This study evaluated the effect of diets differing in standard ileal digestible (SID) lysine on lysine intake, growth rate, body composition and age at puberty on maternal line gilts. Crossbred Large White×Landrace gilts (n =641) were fed corn-soybean diets differing in SID lysine concentration (%, g SID lysine:Mcal ME); diets were not isocaloric. Gilts received three grower, finisher diet combinations: low (0.68% lysine grower, 0.52% lysine finisher), medium (0.79% lysine grower, 0.60% lysine finisher) or high (0.90% lysine grower, 0.68% lysine finisher). Grower diets were fed from 100 until 142 days of age, and finisher diets were fed until they …

Relationships Between Nutrition-Related Knowledge, Self-Efficacy, And Behavior For Fifth Grade Students Attending Title I And Non-Title I Schools, Elisha M. Hall, Weiwen Chai, Julie A. Albrecht

Department of Nutrition and Health Sciences: Faculty Publications

The Social Cognitive Theory (SCT) is a widely used theory for nutrition education programming. Better understanding the relationships between knowledge, self-efficacy, and behavior among children of various income levels can help to form and improve nutrition programs, particularly for socioeconomically disadvantaged youth. The purpose of this study was to determine the relationships between knowledge, self-efficacy, and behavior among fifth grade students attending Title I (≥ 40% of students receiving free or reduced school meals) and non-Title I schools (< 40% of students receiving free or reduced school meals). A validated survey was completed by 55 fifth grade students from Title I and 122 from non-Title I schools. Differences in knowledge, self-efficacy, and behavior scores between groups were assessed using t test and adjusted for variations between participating schools. Regression analysis was used to determine the relationships between knowledge, self-efficacy, and behavior. In adjusted models, the Title I group had significantly lower scores on several knowledge items and summary knowledge (P = 0.04). The Title I group had significantly lower scores on several behavior variables including intakes of fruits (P = 0.02), vegetables (P = 0.0005), whole grains (P = 0.0003), and lean protein (P = 0.047), physical activity (P = 0.002) and summary behavior (P = 0.001). However the Title I group scored higher on self-efficacy for meal planning (P = 0.04) and choosing healthy snacks (P = 0.036). Both self-efficacy (β = 0.70, P < 0.0001) and knowledge (β = 0.35, P = 0.002) strongly predicted behavior; however, only self-efficacy remained significant in the Title I group (self-efficacy, β = 0.82, P = 0.0003; knowledge, β = 0.11, P = 0.59). Results demonstrate disparities in nutrition knowledge and behavior outcomes between students surveyed from Title I and non-Title I schools, suggesting more resources may be necessary for lower income populations. Findings suggest that future nutrition interventions should focus on facilitating the improvement of children's self-efficacy

How Social Reactions To Alcohol-Related Facial Flushing Are Affected By Gender, Relationship, And Drinking Purposes: Implications For Education To Reduce Aerodigestive Cancer Risks, Ian Newman, Lanyan Ding, Duane F. Shell, Lida Lin

Department of Educational Psychology: Faculty Publications

Alcohol-related facial flushing is a sign of compromised alcohol metabolism and increased risk of certain cancers. This project examined how facial flushing might be used to reduce alcohol use to lower cancer risks. Interviews with Chinese university students identified gender, friendship, and drinking purpose as important variables related to whether someone would encourage a person who flushes when drinking alcohol to stop or reduce their drinking. A questionnaire was developed that incorporated these variables into 24 drinking scenarios in which someone flushed while drinking. Students responded whether they would (a) encourage the flusher to stop or drink less; (b) do …

Healthy Food Promotion: The Role Of Information Framing, Cognitive Processes, And Social Influence, Christopher Gustafson

Cornhusker Economics

Rates of overweight and obesity in the U.S. have increased steadily over the past 50 years, with serious consequences for people’s health, livelihoods, and quality of life. Estimates of U.S. adult and childhood overweight and obesity that were based on measurements of height and weight, rather than self-reported data, were collected in a nationwide study conducted in 2011-2012. These data suggest that over one-third of adults were obese and another third were overweight, while 17 percent of children were obese and 15 percent overweight.

Rumen Fluid Metabolomics Analysis Associated With Feed Efficiency On Crossbred Steers, Virginia M. Artegoitia, Andrew P. Cassady, Ronald M. Lewis, Harvey C. Freetly

Department of Animal Science: Faculty Publications

The rumen has a central role in the efficiency of digestion in ruminants. To identify potential differences in rumen function that lead to differences in average daily gain (ADG), rumen fluid metabolomic analysis by LC-MS and multivariate/univariate statistical analysis were used to identify differences in rumen metabolites. Individual feed intake and body-weight was measured on 144 steers during 105 d on a high concentrate ration. Eight steers with the greatest ADG and 8 steers with the least-ADG with dry matter intake near the population average were selected. Blood and rumen fluid was collected from the 16 steers 26 d before …

Assessing Parturition Date Synchrony For North Dakota Ungulates, Eric S. Michel, Jonathan A. Jenks, William F. Jensen

The Prairie Naturalist

Reproductive parameters such as conception date, and therefore parturition date, influence offspring characteristics such as sex ratio and birth mass. For example, late-conceiving reindeer (Rangifer tarandus) produced more female than male offspring compared to those who conceived early (Holand et al. 2006). Females that conceive later and therefore give birth later also can produce lighter offspring compared to those who conceive early (Schwartz et al. 1994, Holand et al. 2006). Because birth mass is positively related to adult body mass (Michel et al. 2015), conception and parturition date likely affect adult phenotype. In turn, this may influence survival because heavy …

Notes: Examining The Potential Competitive Effects Of Ventenata Dubia On Annual And Perennial Grasses, Shawn Mckay, Lesley R. Morris, Christopher E. Morris, Elizabeth A. Leger

The Prairie Naturalist

Portions of the United States are becoming increasingly dominated by annual species, with cheatgrass (Bromus tectorum L.) and medusahead (Taeniatherum caput-medusae [L.] Nevski) as the most common and well-known invaders (Bansal et al. 2014). Other invasive annual species also are present and increasing in abundance, including ventenata (Ventenata dubia [Leers] Coss.), an invasive annual grass that has been expanding within the Pacific Northwest, Great Basin, and Great Plains regions of the United States. Ventenata was first reported in North America in 1952 in Washington (Old and Callihan 1987) and has since spread to 7 western states in the USA (CA, …

Diel And Monthly Movement Rates By Migratory And Resident Female Pronghorn, Paul F. Jones, T. Andrew Hurly, Chelsea Jensen, Karl Zimmer, Andrew Jakes

The Prairie Naturalist

Animal movement patterns are variable, with certain species primarily being diurnal and others nocturnal. Pronghorn (Antilocapra americana) are regarded as diurnal animals moving predominately during daylight hours. Anecdotal accounts, however, suggest that pronghorn move during the night but the extent, frequency, and importance of these nocturnal movement behaviors are unknown. To evaluate movements, we combined global positioning system relocation data from collared female pronghorn in the Northern Sagebrush Steppe between 2003 and 2007 with sunrise/sunset data within a geographical information system platform. We assessed whether mean and maximum movement rates were influenced by diel period (dawn, day, dusk, and night), …

Red Grain Sorghum Whole Kernel Crude Lipid Protects Energy Metabolism And Short Chain Fatty Acid Profile In A Hamster Model To Minimize Intestinal Stress Caused By A High Fat Diet, Haowen Qiu

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Multiple studies have shown throughout the past 10+ years that grain sorghum (GS) lipid extract protects against high plasma and hepatic cholesterol and, to a lesser degree, positively modulate the gut microbiota in response to a high fat (HF) diet. However, the impact of GS lipids on intestinal stress induced by such a diet remains largely unknown. The objective of this project was to determine the ability of GS crude lipid (GS-CL) obtained from the surface of GS whole kernel to protect energy metabolism and short chain fatty acid (SCFA) profile produced by the gut microbiome that may be negatively …

We Cook: Fun With Food And Fitness: Impact Of A Youth Cooking Program On The Home Environment, Courtney Warday

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

BACKGROUND

US food preparation habits have decreased since 1965 (Smith, et al, 2013). Children are rarely involved in food preparation in the home (Fulkerson, et al, 2008). Cooking frequency has been positively associated with healthy eating (Raber, et al, 2016). Food consumption behaviors have changed in the US in the last few decades possibly contributing to the obesity epidemic (Understanding Childhood Obesity, 2010).

OBJECTIVE

The purpose of this study is to examine how We Cook: Fun with Food and Fitness, an elementary youth cooking program impacts the home environment.

PARTICIPANTS

Participants were adult family members of youth involved in the …

Intraguild Interactions And Behavior Of Spodoptera Frugiperda And Helicoverpa, José P. F. Bentivenha, Débora Goulart Montezano, Thomas E. Hunt, Edson L. L. Baldin, Julie A. Peterson, Vinicius S. Victor, Luiz E. R. Pannuti, Ana María Vélez, Silvana L. Vieira De Paula Moraes

Department of Entomology: Faculty Publications

Background: Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae), is one of the major pests of maize, and is in the same feeding guild of the noctuid pests, Helicoverpa zea (Boddie), and H. armigera (Hübner), recently reported in South and North America. The intraguild interactions of these species were assessed in laboratory and field conditions by determining the survival of larvae in interactions scenarios with non-Bt maize silks and ears. Moreover, a video-tracking system was utilized to evaluate behavioral parameters during larval interactions in scenarios with or without food.

Results: In intraguild interactions, S. frugiperda had greater survival (55 …

Nitrogen Addition And Ecosystem Functioning: Changes In Species Composition Leaf Traits Amplify Increases In Leaf Area Index And Canopy Chlorophyll, Anna R. Tatarko

School of Biological Sciences: Dissertations, Theses, and Student Research

Increased nutrient inputs can cause shifts in plant community composition and plant functional traits, both of which affect ecosystem function. We studied community- and species-level changes in specific leaf area (SLA), chlorophyll, leaf thickness, leaf toughness, plant height and leaf dry matter content (LDMC) in a full factorial nitrogen (N), phosphorus (P), potassium (K) fertilization experiment in a semi-arid grassland. Nitrogen was the only nutrient addition to significantly affect leaf functional traits, and N addition increased community weighted SLA by 19%, leaf chlorophyll content by 34%, height by 26%, and resulted in an 11% decrease in LDMC while leaf thickness …

Transcriptomic Resources For The Medicinal Legume Mucuna Pruriens: De Novo Transcriptome Assembly, Annotation, Identification And Validation Of Est-Ssr Markers, N. Sathyanarayana, Ranjith Kumar Pittala, Pankaj Kumar Tripathi, Ratan Chopra, Heikham Russiachand Singh, Vikas Belamkar, Pardeep Kumar Bhardwaj, Jeff J. Doyle, Ashley N. Egan

Department of Agronomy and Horticulture: Faculty Publications

Background:

The medicinal legume Mucuna pruriens (L.) DC. has attracted attention worldwide as a source of the anti-Parkinson’s drug L-Dopa. It is also a popular green manure cover crop that offers many agronomic benefits including high protein content, nitrogen fixation and soil nutrients. The plant currently lacks genomic resources and there is limited knowledge on gene expression, metabolic pathways, and genetics of secondary metabolite production. Here, we present transcriptomic resources for M. pruriens, including a de novo transcriptome assembly and annotation, as well as differential transcript expression analyses between root, leaf, and pod tissues. We also develop microsatellite markers …

Athyrium Multidentatum (Doll.) Ching Extract Induce Apoptosis Via Mitochondrial Dysfunction And Oxidative Stress In Hepg2 Cells, Guoyuan Qi, Zhigang Liu, Rong Fan, Ziru Yin, Yashi Mi, Bo Ren, Xuebo Liu

Department of Nutrition and Health Sciences: Faculty Publications

Athyrium multidentatum (Doll.) Ching (AMC), a unique and nutritious potherb widely distributed in china, has been extensively used in traditional Chinese medicine. Previous studies indicated that AMC extract exhibited antioxidant and antitumor properties. However, the chemical composition of AMC and molecular mechanism of AMC toxicity to HepG2 cells have not yet been elucidated. Hence, this study aimed to investigate the chemical compositions and the underlying mechanisms of the antiproliferative and apoptotic effects of AMC on HepG2. HPLC-MS analysis showed that AMC contain five compounds with chlorogenic acid accounting for 43 percent. Also, AMC strongly inhibited the cell growth and induced …

How An Alloreactive T-Cell Receptor Achieves Peptide And Mhc Specificity, Yuan Wang, Nishant K. Singh, Timothy T. Spear, Lance M. Hellman, Kurt H. Piepenbrink, Rachel H. Mcmahan, Hugo R. Rosen, Craig W. Vander Kooi, Michael I. Nishimura, Brian M. Baker

Food for Health: Publications

T-cell receptor (TCR) allorecognition is often presumed to be relatively nonspecific, attributable to either a TCR focus on exposed major histocompatibility complex (MHC) polymorphisms or the degenerate recognition of allopeptides. However, paradoxically, alloreactivity can proceed with high peptide and MHC specificity. Although the underlying mechanisms remain unclear, the existence of highly specific alloreactive TCRs has led to their use as immunotherapeutics that can circumvent central tolerance and limit graft-versus-host disease. Here, we show how an alloreactive TCR achieves peptide and MHC specificity. The HCV1406 TCR was cloned from T cells that expanded when a hepatitis C virus (HCV)-infected HLA-A2− individual …

The Ne[X]T Generation Learning Environments: Influences On The Future Design Of Educational Spaces, Bryan Hill Perez

Architecture Masters of Science Program: Theses

Over the past 100 years, there have been significant changes in many aspects of our lives, from the way we communicate to the way we work. One aspect that has seen minimal change is the way we learn – In classrooms with rows of desks and chairs facing the front, where a sole instructor disseminates knowledge the class as a whole. As the next generation of students is entering college, it has become apparent that there is a disconnect in the way this generation behaves, interacts, and learns and the typical school infrastructure and pedagogy of today. The design of …

Assessing Landslide Susceptibility With Gis Using Qualitative And Quantitative Methods In Knox County, Nebraska, Christian J. Cruz

Department of Geography: Dissertations, Theses, and Student Research

This thesis assesses landslide susceptibility using data from LiDAR DEMs, land cover, and soil surveys. Data were assessed quantitatively through Bayesian logistic regression within a geographic information system (GIS) and statistical software to produce a landslide susceptibility map. The study area exhibits moderate relief where bluffs along the Missouri River valley gradually recede into rolling loess-mantled hills further to the south and southeast in Knox County. The six factors used to determine susceptibility to landslides are: land cover, parent material, slope aspect, slope curvature slope degree, and soil series. My findings show an increase in slope is the most significant …

Nebline, May 2017

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Biosolids Land Application Program Celebrating 25 Years of Benefiting the Community

Food & Health

Farm & Acreage

Pests & Wildlife

Horticulture

Early Childhood

4-H & Youth

and other extension news and events

Patch Seeding On An Extensive Green Roof: Initial Experiment On Microclimate Impacts, Zhuqing Xu

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The green roof provides not only aesthetic values but also many ecological benefits. In North America, seeding or reseeding with native species becomes increasingly popular as a result of its high efficacy and low cost. The extreme temperature and a lack of water on the green roof impose restrictions on seeds germination that determines the success of a seeding program.

The study started on June 1 and was completed by July 15. Five Great Plains native species were hand-seeded in two blocks on Larson Building green roof, along with two blocks set up in the greenhouse at University of Nebraska-Lincoln …

Heterogeneity Of Avian Breeding Habitat On Grazing Lands Of The Northern Great Plains, Maggi S. Sliwinski

School of Natural Resources: Dissertations, Theses, and Student Research

Native rangelands in the Great Plains are largely privately owned and used for beef production. Vegetation heterogeneity is important for maintaining biodiversity, but private land may be more homogenous than desired. My research had two components: 1) to examine whether a variety of grazing strategies created vegetation heterogeneity in a large, intact rangeland, and 2) to understand beef producers’ attitudes about vegetation heterogeneity.

First, I sampled vegetation structure, composition, and bird abundance at multiple plots on eleven management units in Cherry County, Nebraska. Units were managed with commonly used grazing strategies (e.g., short-duration grazing and season-long continuous grazing). I examined …

University Wellness: A Numbers Game – How Health Numbers Relate To Blood Pressure, Courtney B. Schnell

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

Background: Heart disease is the number one leading cause of death in the United States (CDC, 2016). People who have hypertension are at greater risk for other heart disease (World Heart Federation, 2017). Preventing hypertension through education of health numbers can greatly reduce the hypertension prevalence in the United States.

Objective: Determine the relationship between blood pressure and other health status indicators used in worksite wellness health fairs including; body mass index, waist circumference, stress, and time management.

Design: 156 participants at the University of Nebraska attended a wellness fair, in which blood pressure, body mass index, waist circumference, time …

Answers To Common Misconceptions About Biological Evolution, Leah M. Abebe, Blake Bartels, Kaitlyn M. Caron, Adam M. Gleeson, Samuel N. Johnson, Tyler J. Kluza, Nicholas W. Knopik, Kristen N. Kramer, Masiel S. Maza, Kaitlyn A. Stava, Kaitlyn Sullivan, Jordan T. Trimble, Robert M. Zink

Papers in Evolution

“When you have an established scientific emergent truth, it is true whether or not you believe in it.” Neil deGrasse Tyson, Science in America. Very few people outside of a particular scientific discipline can actually say they understand it, because most do not have the training to “speak the language.” They are then not particularly bothered by its tenets and predictions. Of all the major branches of science, however, evolutionary biology is an exception to this generalization because even though people are not versed in the field, they sometimes have a negative, knee-jerk objection. This objection is often because they …

Atopic Dermatitis And Food Sensitization In South African Toddlers: Role Of Fiber And Gut Microbiota, Mahboobeh Mahdavinia, Heather Rasmussen, Phillip Engen, Jolice P. Van Den Berg, Erika Davis, Krista Engen, Stefan J. Green, Ankur Naqib, Maresa Botha, Claudia Gray, Nonhlanhla Lunjani, Carol Hlela, Wisdom Basera, Lelani Hobane, Alexandra Watkins, Mary C. Tobin, Alan Landay, Ali Keshavarzian, Michael E. Levin

Department of Nutrition and Health Sciences: Faculty Publications

The pathogenesis of atopic dermatitis (AD) is complex and related to allergic responses and defects in skin barrier function. In common with many atopic diseases, the prevalence of AD has been increasing across the world.1 One of the theories for this increase is increased hygiene and urbanization-related changes in the environment, which can affect the human microbiome.2 Previous studies have found associations between the composition of the early gut microbiome and development of atopic conditions, including AD.3 Although the rate of atopic conditions, including AD and food allergy, is increasing on all continents, the prevalence of these diseases is still …

Backgrounding Calf Management Strategies Using Corn Residue And Double Cropped Forages, Jordan L. Cox-O'Neill

Department of Animal Science: Dissertations, Theses, and Student Research

Approximately 70% of calves in the U.S. are born in the spring and weaned in the fall, this results in a large supply of calves potentially available for backgrounding over the winter. Backgrounding systems positively impact the beef industry by efficiently using forage resources available. These systems also provide production value by enhancing the calf’s frame size, mature BW (increase HCW), and health prior to entering the feedlot. In Nebraska opportunities to integrate both crop and livestock production, by backgrounding calves abound. Approximately 13,100 metric tons of corn residue or 34,000 Animal Unit Months are available for grazing in Nebraska. …

Estimates Of Corn Residue Quality, Tasha M. King

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted to determine the effect of corn residue harvest method on animal performance and diet digestibility. Corn residue harvest methods used in these experiments were low-stem, high-stem, and conventional. Steers had greater ADG when fed low-stem in a growing diet compared to high-stem and conventional residues. Addition of supplemental RUP to corn-residue based diets resulted in greater ADG and G:F in steers. Digestibility of DM, OM, and NDF were greatest in lambs fed diets containing low-stem residue. Low-stem residue had greater digestible energy (DE) compared to high-stem and conventional, which did not differ.

Lastly, a study was …

Evaluation Of Protein And Fiber From Distillers’ Grains Plus Solubles In Finishing Beef Cattle Diets, Zachary E. Carlson

Department of Animal Science: Dissertations, Theses, and Student Research

Four studies were conducted to evaluate the components of distillers grains plus solubles in finishing beef cattle diets. Experiment 1 evaluated the effects of five composites of feedstuffs formulated to be similar in nutrient composition to DDGS on finishing performance of cattle. Experiment 2 evaluated isolating the protein from distillers grains using a feedstuff from the wet milling ethanol industry to determine the impacts of protein on the feeding value of WDGS in finishing performance of cattle. Experiments 3 and 4 evaluated the effects of protein from distillers grains with the diets used in Exp. 2 on site of digestion, …

Use Of Byproducts In Forage-Based, Post-Weaning Beef Systems And Effects Of Serial Slaughter On Performance And Profitability, Robert Gregory Bondurant Jr.

Department of Animal Science: Dissertations, Theses, and Student Research

Crude glycerin was included in high-forage beef growing diets at 0, 4, 8, and 12% diet DM to determine the effect on fiber digestion by evaluating changes in microbial species abundance, NDF digestibility, and VFA concentrations. Total tract NDF digestibility decreased with increasing inclusion of GLY in high-forage diets. However, there was no decrease in in situ NDF digestibility and Fibrobacter succinogenes microbial populations were unaffected, indicating that fiber digestion was not directly affected by inclusion of GLY. Acetate decreased while propionate and butyrate VFA proportions increased as GLY increased.

Spayed heifers were utilized in a 2-yr study to evaluate …

Effects Of Energy Restriction During Gilt Development On Milk Nutrient Profile, Progeny Biomarkers, And Growth Performance, Shana Marie Barnett

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted at separate times to evaluate the effects of sow dietary treatment on piglet growth performance. One experiment solely focused on the growth performance of parity 1 piglets from gilt batches 5 through 13. The other experiment focused on growth performance of piglets from batch 14, parity 1, as well as, analysis of growth biomarkers, GLP-2 and Insulin. Milk samples from sows of each dietary treatment were collected and analyzed for oligosaccharide composition, nutrient composition, and insulin levels.

The first experiment utilized 733 sows that were fed either a restricted (RESTR) of ad libitum (ADLIB) diet during …

Effects Of Egg Yolk On Piglet Growth Performance, Circulating Immunoglobulins, Biomarkers Of Intestinal Integrity, And Microbial Community, Kelly C. Moore

Department of Animal Science: Dissertations, Theses, and Student Research

A total of 72 crossbred pigs weaned at 24 days were assigned to a completely randomized design (CRD) arrangement of treatments to determine the effects of egg yolk on a nursery piglet’s growth performance, circulating immunoglobulins, biomarkers of intestinal integrity, and microbial community. Pigs were provided with spray-dried plasma (SDP), spray-dried egg yolk (SDEY), or a control diet. During Phase III, ADG was affected by treatment (P < 0.10). Pigs consuming the egg yolk diet had greater (P < 0.05) ADG vs. the plasma group for Phase III. Circulating immunoglobulins (IgA and IgG) were not affected by dietary treatment. C-reactive protein (CRP) and haptoglobin (Hp) concentrations remained constant during the experiment. Treatment did effect Glugacon like peptide-2 (GLP-2) concentration (P < 0.01) where pigs fed egg yolk had the greatest concentration. Microbial OTUs were similar among treatments (P > 0.8), but there were changes over time; from d 0 to Phase I and II there was an increase in diversity (P < 0.01) followed by a decrease in …

Sensor Placement Effects Acceleration Data For Monitoring Equine Activity, Carol J. Thompson

Department of Animal Science: Dissertations, Theses, and Student Research

Monitoring horse activity continually is a valuable aid for horse caretakers to provide recommendations to help users meet their goals and improve overall horse health. Activity trackers commonly use an accelerometer to determine steps and exercise intensity. The activity level capabilities using a 3-axis accelerometer were tested in three locations on the horse. The objectives were to determine which location results in the most accurate step count, threshold values for each gait and to identify correlations between the thresholds and horse characteristics. Twenty-four horses wore three identical smartphone accelerometers, one in each of three locations: right side of the head …