Digital Commons Network^™

Accumulating Data To Optimally Predict Obesity Treatment (Adopt): Recommendations From The Biological Domain, Michael Rosenbaum, Tanya Agurs-Collins, Molly S. Bray, Kevin D. Hall, Mark Hopkins, Maren Laughlin, Paul S. Maclean, Padma Maruvada, Cary R. Savage, Dana M. Small, Luke Stoeckel

Center for Brain, Biology, and Behavior: Faculty Publications

Background: The responses to behavioral, pharmacological, or surgical obesity treatments are highly individualized. The Accumulating Data to Optimally Predict obesity Treatment (ADOPT) project provides a framework for how obesity researchers, working collectively, can generate the evidence base needed to guide the development of tailored, and potentially more effective, strategies for obesity treatment. Objectives: The objective of the ADOPT biological domain subgroup is to create a list of high-priority biological measures for weight-loss studies that will advance the understanding of individual variability in response to adult obesity treatments. This list includes measures of body composition, energy homeostasis (energy intake and output), …

Generation And Evaluation Of Modified Opaque-2 Popcorn Suggests A Route To Quality Protein Popcorn, Ying Ren, Abou Yobi, Leandra Marshall, Ruthie Angelovici, Oscar Rodriguez, David R. Holding

Center for Plant Science Innovation: Faculty Publications

Introducing traits from dent corn to popcorn is challenging because it is difficult to recover adequate popping characteristics. QPM (Quality Protein Maize) is a dent corn variety carrying the opaque-2 (o2) mutation, specifying increased amounts of normally limiting essential amino acids, and modifier genes which restore the wild type vitreous kernel phenotype. In this study, we introgressed o2 and selected for endosperm modification using vitreousness and high 27-kD gamma zein content. In this way, we recovered high-lysine, fully poppable Quality Protein Popcorn (QPP). BC₂F₄ individuals with vitreous kernels were confirmed to be o2 mutants by …

Molecular Tools Enabling Pennycress (Thlaspi Arvense) As A Model Plant And Oilseed Cash Cover Crop, Michaela Mcginn, Winthrop B. Phippen, Ratan Chopra, Sunil Bansal, Brice A. Jarvis, Mary E. Phippen, Kevin M. Dorn, Maliheh Esfahanian, Tara J. Nazarenus, Edgar B. Cahoon, Timothy P. Durrett, M. David Marks, John C. Sedbrook

Center for Plant Science Innovation: Faculty Publications

Thlapsi arvense L. (pennycress) is being developed as a profitable oilseed cover crop for the winter fallow period throughout the temperate regions of the world, controlling soil erosion and nutrients run-off on otherwise barren farmland. We demonstrate that pennycress can serve as a user-friendly model system akin to Arabidopsis that is well-suited for both laboratory and field experimentation. We sequenced the diploid genome of the spring-type Spring 32-10 inbred line (1C DNA content of 539 Mb; 2n = 14), identifying variation that may explain phenotypic differences with winter-type pennycress, as well as predominantly a one-to-one correspondence with Arabidopsis genes, …

The Role Of Type Iii Effectors From Xanthomonas Axonopodis Pv. Manihotis In Virulence And Suppression Of Plant Immunity, Cesar Augusto Medina, Paola Andrea Reyes, Cesar Augusto Trujillo, Juan Luis Gonzalez, David Alejandro Bejarano, Nathaly Andrea Montenegro, Jonathan M. Jacobs, Anna Joe, Silvia Restrepo, James R. Alfano, Adriana Bernal

Center for Plant Science Innovation: Faculty Publications

Xanthomonas axonopodis pv. manihotis (Xam) causes cassava bacterial blight, the most important bacterial disease of cassava. Xam, like other Xanthomonas species, requires type III effectors (T3Es) for maximal virulence. Xam strain CIO151 possesses 17 predicted T3Es belonging to the Xanthomonas outer protein (Xop) class. This work aimed to characterize nine Xop effectors present in Xam CIO151 for their role in virulence and modulation of plant immunity. Our findings demonstrate the importance of XopZ, XopX, XopAO1 and AvrBs2 for full virulence, as well as a redundant function in virulence between XopN and XopQ in susceptible cassava plants. We tested their role …

Xanthomonas Oryzae Pv. Oryzae Tale Proteins Recruit Ostfiiaγ1 To Compensate For The Absence Of Ostfiiaγ5 In Bacterial Blight In Rice, Wenxiu Ma, Lifang Zou, Zhiyuan Ji, Xiameng Xu, Zhengyin Xu, Yangyang Yang, James R. Alfano

Center for Plant Science Innovation: Faculty Publications

Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of bacterial blight (BB) of rice, uses transcription activator-like effectors (TALEs) to interact with the basal transcription factor gamma subunit OsTFIIAγ5 (Xa5) and activates the transcription of host genes. However, how OsTFIIAγ1, the other OsTFIIAγ protein, functions in the presence of TALEs remains unclear. In this study, we show that OsTFIIAγ1 plays a compensatory role in the absence of Xa5. The expression of OsTFIIAγ1, which is activated by TALE PthXo7, increases the expression of host genes targeted by avirulent and virulent TALEs. Defective OsTFIIAγ1 rice lines show reduced expression of the TALE-targeted …

United States Marine Mammal Commission Annual Report 2018

United States Marine Mammal Commission

United States Marine Mammal Commission Annual Report 2018

Effects Of Tunable, 3d-Bioprinted Hydrogels On Human Brown Adipocyte Behavior And Metabolic Function, Mitchell Kuss, Jiyoung Kim, Dianjun Qi, Shaohua Wu, Yuguo Lei, Soonkyu Chung, Bin Duan

Department of Nutrition and Health Sciences: Faculty Publications

Obesity and its related health complications cause billions of dollars in healthcare costs annually in the United States, and there are yet to be safe and long-lasting anti-obesity approaches. Using brown adipose tissue (BAT) is a promising approach, as it uses fats for energy expenditure. However, the effect of the microenvironment on human thermogenic brown adipogenesis and how to generate clinically relevant sized and functioning BAT are still unknown. In our current study, we evaluated the effects of endothelial growth medium exposure on brown adipogenesis of human brown adipose progenitors (BAP). We found that pre-exposing BAP to angiogenic factors promoted …

Armms As A Versatile Platform For Intracellular Delivery Of Macromolecules, Qiyu Wang, Jiujiu Yu, Tatenda Kadungure, Joseph Beyene, Hong Zhang, Quan Lu

Department of Nutrition and Health Sciences: Faculty Publications

Majority of disease-modifying therapeutic targets are restricted to the intracellular space and are therefore not druggable using existing biologic modalities. The ability to efficiently deliver macromolecules inside target cells or tissues would greatly expand the current landscape of therapeutic targets for future generations of biologic drugs, but remains challenging. Here we report the use of extracellular vesicles, known as arrestin domain containing protein 1 [ARRDC1]-mediated microvesicles (ARMMs), for packaging and intracellular delivery of a myriad of macromolecules, including the tumor suppressor p53 protein, RNAs, and the genome-editing CRISPR-Cas9/guide RNA complex. We demonstrate selective recruitment of these macromolecules into ARMMs. When …

Applying Eye-Tracking Research In Education And Communication To Agricultural Education And Communication: A Review Of Literature, Holli R. Leggette, Amber H. Rice, Candis Carraway, Marshall A. Baker, Nathan W. Conner

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

The purpose of this integrative literature review was to synthesize the eye-tracking literature related to education and communication in the Social Sciences Citation Index (SSCI) to formulate a conceptual model and develop a research agenda that guides eye-tracking research in agricultural education and communication. To do so, we reviewed the literature to identify basic elements of eye-tracking research, examined specific variables related to eye-tracking research, and synthesized the findings into a conceptual model. We found and reviewed 77 articles published between 2010 and 2016 within education (56) and communication (21). Research implications for agricultural education included an examination of classroom …

Effective And Sustainable Prevention Of Avian Damage To Planted Seeds Through Seed Treatment, Jeb A. Barzen, Kenneth E. Ballinger Jr.

Proceedings of the North American Crane Workshop

Several species of cranes and other wildlife have recovered from low populations because, in part, they have adapted to resources found in agricultural environments. If future conservation strategies are to succeed in areas dominated by agricultural use, we must develop sustainable models that solve crop damage problems that are caused by expanding wildlife populations. Using crane damage to planted seed as an example, we propose 1 such model of sustainable crop damage prevention. The deterrent, 9,10-anthraquinone (AQ), is a natural product produced by plants, in part to control bird frugivory, and induces gastro-intestinal distress (temporarily sickens an individual) in sandhill …

Past Role And Future Outlook Of The Conservation Reserve Program For Supporting Honey Bees In The Great Plains, Clint R.V. Otto, Haochi Zheng, Alisa L. Gallant, Rich Iovanna, Benjamin L. Carlson, Matthew D. Smart, Skip Hyberg

United States Geological Survey: Staff Publications

Human dependence on insect pollinators continues to grow even as pollinators face global declines. The Northern Great Plains (NGP), a region often referred to as America’s last honey bee (Apis mellifera) refuge, has undergone rapid land-cover change due to cropland expansion and weakened land conservation programs. We conducted a trend analysis and estimated conversion rates of Conservation Reserve Program (CRP) enrollments around bee apiaries from 2006 to 2016 and developed models to identify areas of habitat loss. Our analysis revealed that NGP apiaries lost over 53% of lands enrolled in the CRP, and the rate of loss was highest in …

Extracellular Superoxide Dismutase (Sod3) Regulates Oxidative Stress At The Vitreoretinal Interface, Katherine J. Wert, Gabriel Velez, Madeline R. Cross, Brett A. Wagner, Melissa L. Teoh-Fitzgerald, Garry R. Buettner, J. Jason Mcanany, Alicia Olivier, Stephen H. Tsang, Matthew M. Harper, Frederick E. Domann, Alexander G. Bassuk, Vinit B. Mahajan

United States Department of Veterans Affairs: Publications

Oxidative stress is a pathogenic feature in vitreoretinal disease. However, the ability of the inner retina to manage metabolic waste and oxidative stress is unknown. Proteomic analysis of antioxidants in the human vitreous, the extracellular matrix opposing the inner retina, identified superoxide dismutase-3 (SOD3) that localized to a unique matrix structure in the vitreous base and cortex. To determine the role of SOD3, Sod3^-/- mice underwent histological and clinical phenotyping. Although the eyes were structurally normal, at the vitreoretinal interface Sod3^-/- mice demonstrated higher levels of 3-nitrotyrosine, a key marker of oxidative stress. Pattern electroretinography also showed physiological …

Nebraska Grazing Conference Proceedings, 2018, Center For Grassland Studies, University Of Nebraska-Lincoln

Nebraska Grazing Conference Proceedings

Pasture Monitoring Sesion

Overview of Choquette Ranch by Jim Choquette

Field Applications of Pasture and Ecological Monitoring by Christine Su

Keeping Human Knowledge at the Center of Technology by Christine Su

Field Monitoring Exercise with Christine Su, Jim O’Rourke, Bethany Johnston, Brad Schick, Brent Plugge, Ben Beckman, and Jace Stott

Prescribed Fire, Bird Monitoring, and Butterfly Date by Andrew Pierson

Strategies for Rangeland Monitoring by Jim O’Rourke

Pasture Monitoring: Field Experience by Jeff Nichols, Beau Mathewson, and Mitch Stephenson

Ranch Succession Planning Session

Keeping Your Ranching Operation in the Family for Future Generations by Ron Hanson

Sharing Our Ranch Succession …

Retail-Based Healthy Food Point-Of-Decision Prompts (Pdps) Increase Healthy Food Choices In A Rural, Low-Income, Minority Community, Christopher Gustafson, Rachel Kent, Michael R. Prate Jr.

Department of Agricultural Economics: Faculty Publications

This study examines the potential for point-of-decision prompts (PDPs) to promote healthier food choices among shoppers in a rural, low-income, minority community. We hypothesized that a narrowly defined PDP (focused on fresh produce) would be easier for shoppers to remember than a broadly defined PDP (focused on any healthy items), resulting in a higher proportion of healthy items purchased. PDPs were placed at the entrance to a supermarket in Mission, South Dakota, United States of America, on the Rosebud Sioux Reservation for alternating time periods, July 9–10, 2017. Sales records from 653 transactions were retrieved from the supermarket, comprising periods …

Profitability Of Developing Beef Heifers On Stockpiled Winter Forages, Zachary David Mcfarlane, Chris Boyer, J. Travis Mulliniks

West Central Research and Extension Center, North Platte

We estimate the profitability of developing heifers on one stockpiled cool-season grass and two stockpiled warm-season grasses during the winter months by comparing distributions of net present value (NPV) over an 11-year useful life. Furthermore, distributions of payback period and the break-even price for each calf over the heifer’s production life were generated for each forage species. These results are compared across forages as well as to a simulated drylot system for heifer development. Data comes from a grazing experiment in Tennessee, where heifers grazed big bluestem and Indian grass combination (BBIG), switchgrass (SW), or endophyte-infected tall fescue (TF) pastures. …

2018 Extension Highlights

Lancaster County, Nebraska Extension: Reports

Nebraska Extension is your local connection to university research-based information. Nebraska Extension in Lancaster County is a partnership between Lancaster County, University of Nebraska–Lincoln and the U.S. Department of Agriculture. We extend knowledge, provide solutions and change lives in these educational program areas: • Community Environment • Food, Nutrition & Health • Cropping & Water Systems • 4-H Youth Development • Learning Child • Beef Systems • Community Vitality

Here’s a look at some of Nebraska Extension in Lancaster County’s 2018 impacts.

Nebline, January 2018

NEBLINE Newsletter Archive from Nebraska Extension in Lancaster County

Feature: Extension 2017 Highlights

Food & Health

Farm & Acreage

Horticulture

Pests & Wildlife

4-H & Youth

Early Childhood

and other extension news and events

A Scalable And Efficient Bioprocess For Manufacturing Human Pluripotent Stem Cell-Derived Endothelial Cells, Haishuang Lin, Qian Du, Qiang Li, Ou Wang, Zhanqi Wang, Neety Sahu, Christian Elowsky, Kan Liu, Chi Zhang, Soonkyu Chung, Bin Duan, Yuguo Lei

Department of Chemical and Biomolecular Engineering: Faculty Publications

Endothelial cells (ECs) are of great value for cell therapy, tissue engineering, and drug discovery. Obtaining high-quantity and -quality ECs remains very challenging. Here, we report a method for the scalable manufacturing of ECs from human pluripotent stem cells (hPSCs). hPSCs are expanded and differentiated into ECs in a 3D thermoreversible PNIPAAm-PEG hydrogel. The hydrogel protects cells from hydrodynamic stresses in the culture vessel and prevents cells from excessive agglomeration, leading to high-culture efficiency including high-viability (>90%), high-purity (>80%), and high-volumetric yield (2.0 x 10⁷ cells/mL). These ECs (i.e., 3D-ECs) had similar properties as ECs made using …

Nebraska Risk And Protective Factor Student Survey Results For 2018. Profile Report: State Of Nebraska, Bureau Of Sociological Research

Nebraska Student Health and Risk Prevention Surveillance System (SHARP)

This report summarizes the findings from the 2014 Nebraska Risk and Protective Factor Student Survey (NRPFSS). The 2014 survey represents the sixth implementation of the NRPFSS and the third implementation of the survey under the Nebraska Student Health and Risk Prevention (SHARP) Surveillance System.

The NRPFSS targets Nebraska students in grades 8, 10, and 12 with a goal of providing schools and communities with local-level data. As a result, the NRPFSS is implemented as a census survey, meaning that every public and non-public school with an eligible grade can choose to participate. Therefore data presented in this report are not …

Widening The Disparity Gap: Differences In Outcomes For Racial And Ethnic Groups In Youth Substance Abuse Prevention Programming, Changsoo Song, Jennifer N. Rutt, Mindy Anderson-Knott

UNL Office of Research and Innovation, Social/Behavioral Sciences Research Consortium

This study examined racial and ethnic differences in the outcomes of a universal evidence-based program targeted to children and adolescents. Using data collected in 2015 and 2016 from the Alcohol: True Stories Hosted by Matt Damon program (N = 925), the study found the outcome of perceptions on risk of harm from alcohol use significantly different between the group of White respondents and the group of non-White respondents, and between the group of Hispanic respondents and the group of non-Hispanic respondents. Program effectiveness was found only among White and non-Hispanic respondents, widening the already existing gap between these populations.

The Honor Society Of Agriculture Gamma Sigma Delta, Nebraska Chapter, History, May 25, 1918-2018

Gamma Sigma Delta, Nebraska Chapter: Bylaws and Other Documents

First paragraph:

GAMMA SIGMA DELTA is a faculty governed society that selects its members from junior and senior students, graduate students, faculty, and alumni for the promotion and recognition of high achievement. While Gamma Sigma Delta is an honor society of agriculture, it may elect members from related fields such as biology, botany, chemistry, economics, entomology, family and consumer sciences, forestry, natural resources, plant pathology, sociology, statistics, veterinary medicine, or zoology when the work of the individual is related to agriculture. Officers, committee chairpersons, and others leading the chapter are faculty or alumni.

Value Of Arrival Metaphylaxis In U.S. Cattle Industry, Elliott James Dennis, Ted C. Schroeder, David G. Renter, Dustin L. Pendell

Department of Agricultural Economics: Faculty Publications

Although several studies have estimated economic impacts of antimicrobials for growth promotion, little is known about economic impacts of the common animal health management strategy known as metaphylaxis: administering antimicrobials to groups of animals to prevent disease. This article develops a new framework to map animal disease to producer profitability and determine societal economic impacts surrounding metaphylactic use of antimicrobials in beef cattle production. Results indicate the direct net return value of metaphylaxis to the U.S. fed cattle industry is at least $532 million. Beef producer surplus losses of $1.8 billion would be associated with eliminating metaphylaxis.

Copd National Action Plan

United States Natonal Institutes of Health: Materials

The COPD National Action Plan is the first-ever blueprint for a multi-faceted, unified fight against the disease. Developed at the request of Congress.

Triennial Reproduction Symposium: Looking Back And Moving Forward—How Reproductive Physiology Has Evolved, Deb L. Hamernik, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

In honor of the 50th anniversary of the Society for the Study of Reproduction (SSR), a Triennial Reproduction Symposium was cosponsored by SSR and the American Society of Animal Science (ASAS). The Society for the Study of Reproduction was formed in 1967 when a group of reproductive biologists that were members of ASAS met with physician scientists and decided to organize a new scientific society. The goal of SSR was to promote the study of reproduction by fostering interdisciplinary communication among scientists, holding conferences, and publishing meritorious studies. Today after its 50th anniversary, the mission for SSR is to harness …

Annual Report: Nebraska Extension In Lancaster County, January 1, 2018 Through December 31, 2018

Lancaster County, Nebraska Extension: Reports

Lancaster County Extension information from 2018. Contents include:

1) About Extension - Lancaster County Extension mission statement, overview and impact reports;

2) Staff and Programs - staff directory and Extension Educator monthly narratives summarizing program highlights;

3) Financials - summaries of 2017-18 budget expenditures and current fiscal year 2018-19 budget; and

4) Media - website and social media information, samples of our monthly newsletter, the Nebline, and local newspaper articles.

Gardening Experience Is Associated With Increased Fruit And Vegetable Intake Among First-Year College Students: A Cross-Sectional Examination, Jennifer Loso, Daniel Staub, Sarah E. Colby, Melissa D. Olfert, Kendra Kattelmann, Melissa Vilaro, James Colee, Wenjun Zhou, Lisa Franzen-Castle, Anne E. Matthews

Department of Nutrition and Health Sciences: Faculty Publications

Background Gardening interventions have been shown to increase fruit and vegetable (F/V) intake among school-aged children. It is unknown whether these effects persist into later adolescence or adulthood, and little is known about whether gardening in later adolescence is related to F/V intake. Objective To identify the relationship between both childhood and recent (within the past 12 months) gardening experiences and current F/V intake among college students. Design/participants A cross-sectional evaluation of 1,121 college freshmen with suboptimal F/V consumption from eight US universities. Main outcome measures Participants completed the National Cancer Institute Fruit and Vegetable Screener and questions about gardening …

Teens Implementing A Childhood Obesity Prevention Program In The Community: Feasibility And Perceptions Of A Partnership With Hsta And Icook 4-H, Rebecca L. Hagedorn, Jade A. White, Lisa Franzen-Castle, Sarah E. Colby, Kendra K. Kattelmann, Adrienne A. White, Melissa D. Olfert

Department of Nutrition and Health Sciences: Faculty Publications

High school student researchers and teen leaders from the Health Science Technology Academy (HSTA), under the supervision of HSTA teachers, led a childhood obesity prevention (COP) program (iCook 4-H). The objective was to evaluate the feasibility and perceptions of having teen leaders implement a COP program for dyads of youth (9–10 years old) and their primary adult food preparer. Behavior change and perceptions were assessed through surveys and open-ended interviews. Across eight HSTA organizations, 43 teen leaders participated in teaching the iCook 4-H program to 24 dyads. Increased frequency of culinary skills, physical activity and mealtime behavior were reported by …

Hidden Hunger: Solutions For America’S Aging Populations, Manfred Eggersdorfer, Ucheoma Akobundu, Regan L. Bailey, Julie Shlisky, Amy R. Beaudreault, Gilles Bergeron, Robert B. Blancato, Jeffrey B. Blumberg, Megan W. Bourassa, Filomena Gomes, Gordon Jensen, Mary Ann Johnson, Douglas Mackay, Keri Marshall, Simin Nikbin Meydani, Katherine L. Tucker

Department of Nutrition and Health Sciences: Faculty Publications

The global population, including the United States, is experiencing a demographic shift with the proportion of older adults (aged ≥ 65 years) growing faster than any other age group. This demographic group is at higher risk for developing nutrition-related chronic conditions such as heart disease and diabetes as well as infections such as influenza and pneumonia. As a result, an emphasis on nutrition is instrumental for disease risk reduction. Unfortunately, inadequate nutrient status or deficiency, often termed hidden hunger, disproportionately affects older adults because of systematic healthcare, environmental, and biological challenges. This report summarizes the unique nutrition challenges facing the …

An Impact Mapping Method To Generate Robust Qualitative Evaluation Of Community-Based Research Programs For Youth And Adults, Melissa D. Olfert, Rebecca L. Hagedorn, Jade A. White, Barbara A. Baker, Sarah E. Colby, Lisa Franzen-Castle, Kendra Kattelmann, Adrienne A. White

Department of Nutrition and Health Sciences: Faculty Publications

Ripple Effect Mapping (REM) is an evaluation approach that has traditionally been used in community settings to visually map the impact of programming and community interventions. This manuscript utilizes the Community Capitals Framework (CCF) to inform REM and to better highlight the changes and impact between various levels of a community, following a childhood obesity prevention intervention. The addition of in-depth qualitative analyses makes this approach particularly useful for the evaluation of interventions with a research–community partnership focus. The objective of this study was to describe a CCF-informed REM approach with detailed protocol, training, and application to the community-based, childhood …

Development Of Igrow: A Curriculum For Youth/Adult Dyads To Increase Gardening Skills, Culinary Competence, And Family Meal Time For Youths And Their Adult Caregivers, Jade A. White, Rebecca L. Hagedorn, Nicole L. Waterland, Makenzie L. Barr, Oluremi A. Famodu, Amy E. Root, Adrienne A. White, Sarah E. Colby, Lisa Franzen-Castle, Kendra Kattelmann, Melissa D. Olfert

Department of Nutrition and Health Sciences: Faculty Publications

This manuscript describes the development of a “learn by actively participating” curriculum for youth and their adult caregivers (dyad pair) to increase gardening skills, culinary competence, and family meal time. The curriculum was developed by integrating “iCook 4-H” and Junior Masters Gardener “Health and Nutrition from the Garden”, and “Essential Elements of 4-H Youth Development” curriculums with additional resources for gardening activities from the USDA’s My Plate and garden-based recipes. Expert reviewers (n = 11) provided feedback on the curriculum content, session structure, dosage, age appropriateness, and balance of the three focused areas. Seven family dyads (n = …