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Soybean Nodule-Associated Non-Rhizobial Bacteria Inhibit Plant Pathogens And Induce Growth Promotion In Tomato, Serkan Tokgöz, Dilip K. Lakshman, Mahmoud H. Ghozlan, Hasan Pinar, Daniel P. Roberts, Amitava Mitra Jan 2020

Soybean Nodule-Associated Non-Rhizobial Bacteria Inhibit Plant Pathogens And Induce Growth Promotion In Tomato, Serkan Tokgöz, Dilip K. Lakshman, Mahmoud H. Ghozlan, Hasan Pinar, Daniel P. Roberts, Amitava Mitra

Department of Agronomy and Horticulture: Faculty Publications

The root nodules are a unique environment formed on legume roots through a highly specific symbiotic relationship between leguminous plants and nodule-inducing bacteria. Previously, Rhizobia were presumed to be the only group of bacteria residing within nodules. However, recent studies discovered diverse groups of bacteria within the legume nodules. In this report soybean nodule-associated bacteria were studied in an effort to identify beneficial bacteria for plant disease control and growth promotion. Analysis of surface-sterilized single nodules showed bacterial diversity of the nodule microbiome. Five hundred non-rhizobial colonies from 10 nodules, 50 colonies per nodule, were tested individually against the tomato …


Foliar Micronutrient Application For High-Yield Maize, Zachary P. Stewart, Ellen T. Paparozzi, Charles S. Wortmann, Prakash Kumar Jha, Charles A. Shapiro Jan 2020

Foliar Micronutrient Application For High-Yield Maize, Zachary P. Stewart, Ellen T. Paparozzi, Charles S. Wortmann, Prakash Kumar Jha, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

Nebraska soils are generally micronutrient sufficient. However, critical levels for current yields have not been validated. From 2013 to 2015, 26 on-farm paired comparison strip-trials were conducted across Nebraska to test the effect of foliar-applied micronutrients on maize (Zea mays L.) yield and foliar nutrient concentrations. Treatments were applied from V6 to V14 at sites with 10.9 to 16.4 Mg ha−1 yield. Soils ranged from silty clays to fine sands. Soil micronutrient availability and tissue concentrations were all above critical levels for deficiency. Significant grain yield increases were few. Micronutrient concentrations for leaf growth that occurred after foliar applications were …


Continuous Cropping Alters Multiple Biotic And Abiotic Indicators Of Soil Health, Pervaiz Ahmad, Javed Iqbal, Qingming Zhang, Dima Chen, Hui Wei, Muhammad Saleem Jan 2020

Continuous Cropping Alters Multiple Biotic And Abiotic Indicators Of Soil Health, Pervaiz Ahmad, Javed Iqbal, Qingming Zhang, Dima Chen, Hui Wei, Muhammad Saleem

Department of Agronomy and Horticulture: Faculty Publications

The continuous cropping (CC) of major agricultural, horticultural, and industrial crops is an established practice worldwide, though it has significant soil health-related concerns. However, a combined review of the effects of CC on soil health indicators, in particular omics ones, remains missing. The CC may negatively impact multiple biotic and abiotic indicators of soil health, fertility, and crop yield. It could potentially alter the soil biotic indicators, which include but are not limited to the composition, abundance, diversity, and functioning of soil micro- and macro-organisms, microbial networks, enzyme activities, and soil food web interactions. Moreover, it could also alter various …


Coal Char Affects Soil Ph To Reduce Ammonia Volatilization From Sandy Loam Soil, Dinesh Panday, Maysoon M. Mikha, Bijesh Maharjan Jan 2020

Coal Char Affects Soil Ph To Reduce Ammonia Volatilization From Sandy Loam Soil, Dinesh Panday, Maysoon M. Mikha, Bijesh Maharjan

Department of Agronomy and Horticulture: Faculty Publications

Ammonia (NH3) volatilization loss adversely affects N availability in soil-plant systems, reduces crop yield, and negatively impacts environment. Char (coal combus- tion residue), which contains up to 293 g kg−1 total C by weight, has been shown to reduce NH3 volatilization due to its considerably high surface area and cation exchange capacity. The NH3 loss can be greatly affected by a shift in soil pH or urea hydrolysis. A 21-d laboratory study was conducted to evaluate the effects of char on soil pH, N transformations, and subsequent NH3 volatilization in sandy loam soil. Two char rates (0 and 13.4 Mg …


Adjusting Corn Nitrogen Management By Including A Mineralizable-Nitrogen Test With The Preplant And Presidedress Nitrate Tests, Jason Clark, Fabián G. Fernández, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, C. A.M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan Jan 2020

Adjusting Corn Nitrogen Management By Including A Mineralizable-Nitrogen Test With The Preplant And Presidedress Nitrate Tests, Jason Clark, Fabián G. Fernández, Kristen S. Veum, James J. Camberato, Paul R. Carter, Richard Ferguson, David W. Franzen, Daniel E. Kaiser, Newell R. Kitchen, C. A.M. Laboski, Emerson Nafziger, Carl J. Rosen, John E. Sawyer, John F. Shanahan

Department of Agronomy and Horticulture: Faculty Publications

The anaerobic potentially mineralizable N (PMN) test combined with the preplant (PPNT) and presidedress (PSNT) nitrate tests may improve corn (Zea mays L.) N fertilization predictions. Forty-nine corn N response experiments (mostly corn following soybean [Glycine max (L.) Merr.]) were conducted in the U.S. Midwest from 2014–2016 to evaluate the ability of the PPNT and PSNT to predict corn relative yield (RY) and N fertilizer over- and under-application rates when adjusted by PMN. Before planting and N fertilization, PPNT (0–30, 30–60, and 60–90 cm) and PMN (0–30 cm) samples were obtained. In-season soil samples were obtained at the …


Relating Four-Day Soil Respiration To Corn Nitrogen Fertilizer Needs Across 49 U.S. Midwest Fields, G. M. Bean, Newell R. Kitchen, Kristen S. Veum, James J. Camberato, Richard Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson Nafziger, John E. Sawyer, Matt Yost Jan 2020

Relating Four-Day Soil Respiration To Corn Nitrogen Fertilizer Needs Across 49 U.S. Midwest Fields, G. M. Bean, Newell R. Kitchen, Kristen S. Veum, James J. Camberato, Richard Ferguson, Fabian G. Fernandez, David W. Franzen, Carrie A.M. Laboski, Emerson Nafziger, John E. Sawyer, Matt Yost

Department of Agronomy and Horticulture: Faculty Publications

Soil microbes drive biological functions thatmediate chemical and physical processes necessary for plants to sustain growth. Laboratory soil respiration has been proposed as one universal soil health indicator representing these functions, potentially informing crop and soil management decisions. Research is needed to test the premise that soil respiration is helpful for profitable in-season nitrogen (N) rate management decisions in corn (Zea mays L.). The objective of this research was two-fold: (i) determine if the amount of N applied at the time of planting effected soil respiration, and (ii) evaluate the relationship of soil respiration to corn yield response to …


Effects Of Wild Pig Disturbance On Forest Vegetation And Soils, Steven M. Gray, Gary J. Roloff, Daniel B. Kramer, Dwayne R. Etter, Kurt C. Vercauteren, Robert A. Montgomery Jan 2020

Effects Of Wild Pig Disturbance On Forest Vegetation And Soils, Steven M. Gray, Gary J. Roloff, Daniel B. Kramer, Dwayne R. Etter, Kurt C. Vercauteren, Robert A. Montgomery

United States Department of Agriculture Wildlife Services: Staff Publications

In North America, wild pigs (Sus scrofa; feral pigs, feral swine, wild boars) are a widespread exotic species capable of creating large‐scale biotic and abiotic landscape perturbations. Quantification of wild pig environmental effects has been particularly problematic in northern climates, where they occur only recently as localized populations at low densities. Between 2016 and 2017, we assessed short‐term (within ~2 yrs of disturbance) effects of a low‐density wild pig population on forest features in the central Lower Peninsula of Michigan, USA. We identified 16 8‐ha sites using global positioning system locations from 7 radio‐collared wild pigs for sampling.Within each …


Effects Of Brodifacoum And Diphacinone Exposure On Four Species Of Reptiles: Tissue Residue Levels And Survivorship, Richard E. Mauldin, Gary W. Witmer, S. A. Shriner, Rachael S. Moulton, Katherine E. Horak Jan 2020

Effects Of Brodifacoum And Diphacinone Exposure On Four Species Of Reptiles: Tissue Residue Levels And Survivorship, Richard E. Mauldin, Gary W. Witmer, S. A. Shriner, Rachael S. Moulton, Katherine E. Horak

United States Department of Agriculture Wildlife Services: Staff Publications

BACKGROUND: Anticoagulant rodenticides are used worldwide to control pest rodent species. However, the risks posed to nontarget reptiles have not been well characterized. In this study, 46 giant ameivas (Ameiva ameiva), 39 boa constrictors (Boa constrictor), 33 wood turtles (Rhinoclemmys pulcherrima), and 47 green iguanas (Iguana iguana) were orally dosed with one of two levels of either diphacinone or brodifacoum anticoagulant in propylene glycol solutions. Dosages were derived using daily food intake (DFI) equations, converting DFI to an equivalent anticoagulant bait amount and gavaging the solution volume needed to deliver the quantity of anticoagulant in that amount of bait. Animals …


Multi-Isotopic (Δ2h, Δ13c, Δ15n) Tracing Of Molt Origin For European Starlings Associated With U.S. Dairies And Feedlots, Scott Werner, J. W. Fischer, Keith A. Hobson Jan 2020

Multi-Isotopic (Δ2h, Δ13c, Δ15n) Tracing Of Molt Origin For European Starlings Associated With U.S. Dairies And Feedlots, Scott Werner, J. W. Fischer, Keith A. Hobson

United States Department of Agriculture Wildlife Services: Staff Publications

Introduced bird species can become invasive in agroecosystems and their management is inhibited if their origin and movements are not well understood. Stable isotope measurements of feathers can be used to infer molt origins and interstate movements in North America. We analyzed stable-hydrogen (δ2H), carbon (δ13C) and nitrogen (δ 15N) isotope ratios in feathers to better understand the molt origin of European starlings (Sturnus vulgaris) collected at dairies and feedlots throughout the United States. Primary feathers were used from 596 adult and 90 juvenile starlings collected during winter at dairies …


Historic And Contemporary Use Of Catfish Aquaculture By Piscivorous Birds In The Mississippi Delta, Paul C. Burr, Jimmy L. Avery, Garrett M. Street, Bronson K. Strickland, Brian S. Dorr Jan 2020

Historic And Contemporary Use Of Catfish Aquaculture By Piscivorous Birds In The Mississippi Delta, Paul C. Burr, Jimmy L. Avery, Garrett M. Street, Bronson K. Strickland, Brian S. Dorr

United States Department of Agriculture Wildlife Services: Staff Publications

Piscivorous birds are the primary source of catfish (Ictalurus spp.) depredation at aquaculture facilities in northwestern Mississippi. Of particular concern is the Double-crested Cormorant (Phalacrocorax auritus), which can cost aquaculture producers millions of dollars annually through the depredation of cultured fish. Historical research conducted in the early 2000s estimated cormorant use of aquaculture ponds in the region, but aquaculture area has decreased by more than 70% since those estimates were made. With less aquaculture available, we predicted cormorant densities on aquaculture would be greater today than historically. Applying a similar methodology as in historical studies, we used …


Draft Genome Assembly Of Passalora Sequoiae A Needle Blight Pathogen On Leyland Cypress, Warren E. Copes, Jorge Ibarra Caballero, Ebrahiem Babiker, Jane E. Stewart, Valerie A. Orner, Alan S. Windham, Renee Arias Jan 2020

Draft Genome Assembly Of Passalora Sequoiae A Needle Blight Pathogen On Leyland Cypress, Warren E. Copes, Jorge Ibarra Caballero, Ebrahiem Babiker, Jane E. Stewart, Valerie A. Orner, Alan S. Windham, Renee Arias

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Objective: Passalora sequoiae (family Mycosphaerellaceae) causes a twig blight on Leyland cypress that requires numerous fungicide applications annually to minimize economic losses for ornamental plant nursery and Christmas tree producers. The objective was to generate a high-quality draft assembly of the genome of P. sequoiae as a resource for primer development to investigate genotype diversity. Data description: We report here the genome sequence of P. sequoiae 9LC2 that was isolated from Leyland cypress ‘Leighton Green’ in 2017 in southern Mississippi, USA. The draft genome was obtained using Pacific Biosciences (PacBio) SMRT and Illumina HiSeq 2500 sequencing. Illumina reads were mapped …


Brassinosteroids Inhibit Autotropic Root Straightening By Modifying Filamentous-Actin Organization And Dynamics, Louise De Bang, Ana Paez-Garcia, Ashley E. Cannon, Sabrina Chin, Jaydeep Kolape, Fuqi Liao, J. Alan Sparks, Qingzhen Jiang, Elison B. Blancaflor Jan 2020

Brassinosteroids Inhibit Autotropic Root Straightening By Modifying Filamentous-Actin Organization And Dynamics, Louise De Bang, Ana Paez-Garcia, Ashley E. Cannon, Sabrina Chin, Jaydeep Kolape, Fuqi Liao, J. Alan Sparks, Qingzhen Jiang, Elison B. Blancaflor

Nebraska Center for Biotechnology: Faculty and Staff Publications

When positioned horizontally, roots grow down toward the direction of gravity. This phenomenon, called gravitropism, is influenced by most of the major plant hormones including brassinosteroids. Epi-brassinolide (eBL) was previously shown to enhance root gravitropism, a phenomenon similar to the response of roots exposed to the actin inhibitor, latrunculin B (LatB). This led us to hypothesize that eBL might enhance root gravitropism through its effects on filamentous-actin (F-actin). This hypothesis was tested by comparing gravitropic responses of maize (Zea mays) roots treated with eBL or LatB. LatB- and eBL-treated roots displayed similar enhanced downward growth compared with controls …


Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jin Nakashima, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang Jan 2020

Functional Characterization Of Petiolule-Like Pulvinus (Plp) Gene In Abscission Zone Development In Medicago Truncatula And Its Application To Genetic Improvement Of Alfalfa, Juan Du, Shaoyun Lu, Maofeng Chai, Chuanen Zhou, Liang Sun, Yuhong Tang, Jin Nakashima, Jaydeep Kolape, Zhaozhu Wen, Marjan Behzadirad, Tianxiu Zhong, Juan Sun, Yunwei Zhang, Zeng-Yu Wang

Nebraska Center for Biotechnology: Faculty and Staff Publications

Alfalfa (Medicago sativa L.) is one of the most important forage crops throughout the world. Maximizing leaf retention during the haymaking process is critical for achieving superior hay quality and maintaining biomass yield. Leaf abscission process affects leaf retention. Previous studies have largely focused on the molecular mechanisms of floral organ, pedicel and seed abscission but scarcely touched on leaf and petiole abscission. This study focuses on leaf and petiole abscission in the model legume Medicago truncatula and its closely related commercial species alfalfa. By analysing the petiolule-like pulvinus (plp) mutant in M. truncatula at phenotypic level (breakstrength and …


An Ortholog Of The Vasa Intronic Gene Is Required For Small Rna-Mediated Translation Repression In Chlamydomonas Reinhardtii, Xinrong Ma, Fadia Ibrahim, Eun-Jeong Kim, Scott Shaver, James Becker, Fareha Razvi, Ronald L. Cerny, Heriberto D. Cerutti Jan 2020

An Ortholog Of The Vasa Intronic Gene Is Required For Small Rna-Mediated Translation Repression In Chlamydomonas Reinhardtii, Xinrong Ma, Fadia Ibrahim, Eun-Jeong Kim, Scott Shaver, James Becker, Fareha Razvi, Ronald L. Cerny, Heriberto D. Cerutti

School of Biological Sciences: Faculty Publications

Small RNAs (sRNAs) associate with Argonaute (AGO) proteins in effector complexes, termed RNA-induced silencing complexes (RISCs), which regulate complementary transcripts by translation inhibition and/or RNA degradation. In the unicellular alga Chlamydomonas, several metazoans, and land plants, emerging evidence indicates that polyribosome-associated transcripts can be translationally repressed by RISCs without substantial messenger RNA (mRNA) destabilization. However, the mechanism of translation inhibition in a polyribosomal context is not understood. Here we show that Chlamydomonas VIG1, an ortholog of the Drosophila melanogaster Vasa intronic gene (VIG), is required for this process. VIG1 localizes predominantly in the cytosol and comigrates with monoribosomes and …


Coproid Predicts The Source Of Coprolites And Paleofeces Using Microbiome Composition And Host Dna Content, Maxime Borry, Bryan Cordova, Angela Perri, Marsha Wibowo, Tanvi Prasad Honap, Jada Ko, Kate Britton, Linus Girdland-Flink, Robert C. Power, Ingelise Stuijts, Domingo C. Salazar-García, Courtney Hofman, Richard Hagan, Thérèse Samdapawindé Kagoné, Nicolas Meda, Helene Carabin, David Jacobson, Karl Reinhard, Cecil Lewis, Aleksandar Kostic, Choongwon Jeong, Alexander Herbig, Alexander Hübner, Christina Warinner Jan 2020

Coproid Predicts The Source Of Coprolites And Paleofeces Using Microbiome Composition And Host Dna Content, Maxime Borry, Bryan Cordova, Angela Perri, Marsha Wibowo, Tanvi Prasad Honap, Jada Ko, Kate Britton, Linus Girdland-Flink, Robert C. Power, Ingelise Stuijts, Domingo C. Salazar-García, Courtney Hofman, Richard Hagan, Thérèse Samdapawindé Kagoné, Nicolas Meda, Helene Carabin, David Jacobson, Karl Reinhard, Cecil Lewis, Aleksandar Kostic, Choongwon Jeong, Alexander Herbig, Alexander Hübner, Christina Warinner

Karl Reinhard Publications

Shotgun metagenomics applied to archaeological feces (paleofeces) can bring new insights into the composition and functions of human and animal gut microbiota from the past. However, paleofeces often undergo physical distortions in archaeological sediments, making their source species difficult to identify on the basis of fecal morphology or microscopic features alone. Here we present a reproducible and scalable pipeline using both host and microbial DNA to infer the host source of fecal material. We apply this pipeline to newly sequenced archaeological specimens and show that we are able to distinguish morphologically similar human and canine paleofeces, as well as non-fecal …


Diet Analysis Reveals Pre-Historic Meals Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos, Rio Zape, Mexico (600–800 Ce), Elisa Pucu, Julia Russ, Karl Reinhard Jan 2020

Diet Analysis Reveals Pre-Historic Meals Among The Loma San Gabriel At La Cueva De Los Muertos Chiquitos, Rio Zape, Mexico (600–800 Ce), Elisa Pucu, Julia Russ, Karl Reinhard

Karl Reinhard Publications

Coprolites have been a source of study for archeologists due to several reasons: they not only provide information on the life and nutritional habits of ancient individuals but also on their health. In this paper, we processed 10 coprolites collected at La Cueva de Los Muertos Chiquitos (600–800 CE), Rio Zape, Mexico, with acetolysis solution for pollen analysis. The number of pollen grains/gram of each coprolite sample was quantified along with the macroscopic remains of these samples. The main food item ingested by the population was maize, followed by Agave. Squash blossoms were also part of their food source …


Correspondence Between Cytomegalovirus Immunoglobulin-G Levels Measured In Saliva And Serum, Jenna L. Riis, Hedyeh Ahmadi, Olivia Silke, Steve W. Granger, Crystal I. Bryce, Douglas A. Granger Jan 2020

Correspondence Between Cytomegalovirus Immunoglobulin-G Levels Measured In Saliva And Serum, Jenna L. Riis, Hedyeh Ahmadi, Olivia Silke, Steve W. Granger, Crystal I. Bryce, Douglas A. Granger

Department of Psychology: Faculty Publications

Human cytomegalovirus (HCMV) infects more than 80% of the global population. While mostly asymptomatic, HCMV infection can be serious among the immunocompromised, and it is implicated in chronic disease pathophysiology in adulthood. Large-scale minimally invasive HCMV screening could advance research and public health efforts to monitor infection prevalence and prevent or mitigate downstream risks associated with infection. We examine the utility of measuring HCMV immunoglobulin-G (IgG) levels in saliva as an index of serum levels. Matched serum and saliva samples from healthy adults (N = 98; 44% female; 51% white) were assayed for HCMV IgG, total salivary protein, and salivary …


Association Of Home Food Availability With Prediabetes And Diabetes Among Adults In The United States, Jennifer R. Mcatee, Meng-Hua Tao, Christian King, Weiwen Chai Jan 2020

Association Of Home Food Availability With Prediabetes And Diabetes Among Adults In The United States, Jennifer R. Mcatee, Meng-Hua Tao, Christian King, Weiwen Chai

Department of Nutrition and Health Sciences: Faculty Publications

This study examined associations of home food availabilities with prediabetes and diabetes among 8929 adults (20–70 years) participating in 2007–2010 National Health and Nutrition Examination Surveys. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were estimated by logistic regression. Relative to non-diabetic participants (individuals without diabetes or prediabetes), prediabetes participants were associated with lower availabilities of green vegetables (OR = 0.82; 95% CI = 0.73–0.91; p = 0.0006) and fat-free/low-fat milk (OR = 0.80, 95% CI = 0.65–0.89; p = 0.001) and higher sugary drink availability (OR = 1.24, 95% CI = 1.04–1.48; p = 0.02), adjusting for age, …


Listening To The Voices Of Community Health Workers: A Multilevel, Culture-Centered Approach To Overcoming Structural Barriers In U.S. Latinx Communities, Angela L. Palmer-Wackerly, Virginia Chaidez, Caitlyn Wayment, Jonathan Baker, Anthony Adams, Lorey A. Wheeler Jan 2020

Listening To The Voices Of Community Health Workers: A Multilevel, Culture-Centered Approach To Overcoming Structural Barriers In U.S. Latinx Communities, Angela L. Palmer-Wackerly, Virginia Chaidez, Caitlyn Wayment, Jonathan Baker, Anthony Adams, Lorey A. Wheeler

Department of Nutrition and Health Sciences: Faculty Publications

Community Health Workers (CHWs) are often incorporated into efforts to reduce health disparities for vulnerable populations. However, their voices are rarely the focus of research when considering how to increase their job effectiveness and sustainability. The current study addresses this gap by privileging the voices of 28 CHWs who work with Latinx communities in Nebraska through in-depth, semi-structured interviews. Using a multilevel, Culture-Centered Approach (CCA) to Health Communication, we identified two key structural communication issues: (a) increasing language accommodation and (b) increasing (and stabilizing) network integration across three ecological levels of health behavior (individual, microsystem, and exosystem …


Endogenous Versus Exogenous Carbohydrate Oxidation Measured By Stable Isotopes In Pre-Pubescent Children Plus 13c Abundances In Foods Consumed Three Days Prior, Marni E. Shoemaker, Zachary M. Gillen, Brianna D. Mckay, Todd J. Leutzinger, Vikkie A. Mustad, Joel T. Cramer Jan 2020

Endogenous Versus Exogenous Carbohydrate Oxidation Measured By Stable Isotopes In Pre-Pubescent Children Plus 13c Abundances In Foods Consumed Three Days Prior, Marni E. Shoemaker, Zachary M. Gillen, Brianna D. Mckay, Todd J. Leutzinger, Vikkie A. Mustad, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

Purpose: The purposes of the present study were to (a) examine resting metabolism, substrate utilization, and endogenous versus exogenous carbohydrate (CHO) oxidation before and after 30-g rapidly-digesting carbohydrate (RDC) ingestion using indirect calorimetry and breath test analysis of stable isotope concentrations in pre-pubescent children and (b) report the 13C abundances in foods consumed for three days prior.

Methods: Nineteen children (n 1⁄4 10 boys, n 1⁄4 9 girls) at Tanner stage I or II participated (mean age ± 95% CI 1⁄4 9.84 ± 0.77 y) in this study. Food was administered to the children for three days preceding their scheduled …


Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rahel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattlemann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby Jan 2020

Eb4cast Approach Improves Science Communication With Stakeholders In A College-Based Health Program, Melissa D. Olfert, Makenzie L. Barr, Rebecca L. Hagedorn, Rahel A. Wattick, Wenjun Zhou, Tanya M. Horacek, Anne E. Mathews, Kendra K. Kattlemann, Tandalayo Kidd, Adrienne A. White, Onikia N. Brown, Jesse Stabile Morrell, Lisa Franzen-Castle, Karla P. Shelnutt, Carol Byrd-Bredbenner, Terezie Tolar-Peterson, Geoffrey W. Greene, Sarah E. Colby

Department of Nutrition and Health Sciences: Faculty Publications

Communicating scientific results with community partners is often lacking in intervention programs, thus eB4CAST was developed to facilitate impact sharing. This article investigated using the eB4CAST dissemination tool to communicate impact from a campus-based obesity prevention program. Data from Get Fruved RCT university sites collected at baseline were used to generate eB4CAST reports. Experts (n = 13) and RCT sites (n = 15) were asked to provide feedback on eB4CAST reports based on appeal, understanding, and clarity. On all Likert items, participants rated above 7 on each (out of 10). Positive responses from open-ended questions included eB4CAST reports being clear, …


Application Of The Neuromuscular Fatigue Threshold Treadmill Test To Muscles Of The Quadriceps And Hamstrings, Clayton L. Camic, Attila J. Kovacs, Trisha A. Vandusseldorp, Ethan C. Hill Phd, Evan A. Enquist Jan 2020

Application Of The Neuromuscular Fatigue Threshold Treadmill Test To Muscles Of The Quadriceps And Hamstrings, Clayton L. Camic, Attila J. Kovacs, Trisha A. Vandusseldorp, Ethan C. Hill Phd, Evan A. Enquist

Department of Nutrition and Health Sciences: Faculty Publications

Purpose: The purposes of the present study were: (1) to determine whether the physical working capacity at the fatigue threshold (PWCFT) model that has been used for estimating the onset of neuromuscular fatigue in the vastus lateralis (VL) during incremental treadmill running could also be applied to the vastus medialis (VM), biceps femoris (BF), and semitendinosus (ST) muscles; and (2) if applicable, to compare the running velocities associated with the PWCFT among these muscles.

Methods: Eleven subjects (age 21.7 ± 1.8 years) performed an incremental treadmill test to exhaustion with electromyographic signals recorded from the …


Ipsilateral And Contralateral Torque Responses To Bilateral And Unilateral Maximal, Fatiguing, Isokinetic Leg Extensions, Tyler J. Neltner, John Paul V. Anders, Joshua L. Keller, Robert W. Smith, Terry J. Housh, Richard J. Schmidt, Glen O. Johnson Jan 2020

Ipsilateral And Contralateral Torque Responses To Bilateral And Unilateral Maximal, Fatiguing, Isokinetic Leg Extensions, Tyler J. Neltner, John Paul V. Anders, Joshua L. Keller, Robert W. Smith, Terry J. Housh, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Background: Few studies have compared performance fatigability (PF) for bilateral versus unilateral isokinetic tasks. Objectives: The purpose of this study was to examine: Mode- specific testing responses to isokinetic fatigue, differences in PF between bilateral and unilateral leg extensions, and the effects of fatiguing, unilateral, dynamic leg extensions on contralateral isokinetic peak torque (PT) and maximal voluntary isometric contraction (MVIC). Methods: Eight men (mean ± SD: age= 22.5 ± 2.5 yr.) completed pre- and post-testing for PT and MVIC following 50 bilateral, unilateral right or left leg maximal, isokinetic leg extensions at 180°·s-1, on three separate days. Fatigue-induced decreases in …


Application Of The Neuromuscular Fatigue Threshold Treadmill Test To Muscles Of The Quadriceps And Hamstrings, Clayton L. Camic, Attila J. Kovacs, Trisha A. Vandusseldorp, Ethan C. Hill, Evan A. Enquist Jan 2020

Application Of The Neuromuscular Fatigue Threshold Treadmill Test To Muscles Of The Quadriceps And Hamstrings, Clayton L. Camic, Attila J. Kovacs, Trisha A. Vandusseldorp, Ethan C. Hill, Evan A. Enquist

Department of Nutrition and Health Sciences: Faculty Publications

Purpose: The purposes of the present study were: (1) to determine whether the physical working capacity at the fatigue threshold (PWCFT) model that has been used for estimating the onset of neuromuscular fatigue in the vastus lateralis (VL) during incremental treadmill running could also be applied to the vastus medialis (VM), biceps femoris (BF), and semitendinosus (ST) muscles; and (2) if applicable, to compare the running velocities associated with the PWCFT among these muscles.

Methods: Eleven subjects (age 21.7 § 1.8 years) performed an incremental treadmill test to exhaustion with electromyographic signals recorded from the VL, VM, BF, and ST. …


A Mouse Model Of Human Tlr4 D299g/T399i Snps Reveals Mechanisms Of Altered Lps And Pathogen Responses, Katharina Richard, Kurt H. Piepenbrink, Kari Ann Shirey, Archana Gopalakrishnan, Shreeram Nallar, Daniel J. Prantner, Darren J. Perkins, Wendy Lai, Alexandra Vik, Vladimir Y. Toshchakov, Chiguang Feng, Rachel Fanaroff, Andrei E. Medvedev, Jorge C.G. Blanco, Stefanie N. Vogel Jan 2020

A Mouse Model Of Human Tlr4 D299g/T399i Snps Reveals Mechanisms Of Altered Lps And Pathogen Responses, Katharina Richard, Kurt H. Piepenbrink, Kari Ann Shirey, Archana Gopalakrishnan, Shreeram Nallar, Daniel J. Prantner, Darren J. Perkins, Wendy Lai, Alexandra Vik, Vladimir Y. Toshchakov, Chiguang Feng, Rachel Fanaroff, Andrei E. Medvedev, Jorge C.G. Blanco, Stefanie N. Vogel

Department of Food Science and Technology: Faculty Publications

Two cosegregating single-nucleotide polymorphisms (SNPs) in human TLR4, an A896G transition at SNP rs4986790 (D299G) and a C1196T transition at SNP rs4986791 (T399I), have been associated with LPS hyporesponsiveness and differential susceptibility to many infectious or inflammatory diseases. However, many studies failed to confirm these associations, and transfection experiments resulted in conflicting conclusions about the impact of these SNPs on TLR4 signaling. Using advanced protein modeling from crystallographic data of human and murine TLR4, we identified homologous substitutions of these SNPs in murine Tlr4, engineered a knock-in strain expressing the D298G and N397I TLR4 SNPs homozygously, and characterized in vivo …


Forage Systems For The Temperate Subhumid And Semiarid Areas, John R. Hendrickson, Corey Moffet Jan 2020

Forage Systems For The Temperate Subhumid And Semiarid Areas, John R. Hendrickson, Corey Moffet

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

No abstract provided.


Peer Review Of Teaching Portfolio For Hort 306: Greenhouse Operations & Management, Stacy Adams Jan 2020

Peer Review Of Teaching Portfolio For Hort 306: Greenhouse Operations & Management, Stacy Adams

UNL Faculty Course Portfolios

A Greenhouse Operations and Management course designed specifically to serve the needs of horticulture students at an institution of higher learning has transitioned teaching philospophy to additionally serve any student with a potential use of a greenhouse in instruction, community outreach, research, extension, early childhood development, and thearapy. Through the Peer Review of Teaching Project, the course is explored, analyzed and redeveloped with intentional purpose.


Fdst 880p: Food Proteins - A Peer Review Of Teaching Project, Benchmark Portfolio, Kaustav Majumder Jan 2020

Fdst 880p: Food Proteins - A Peer Review Of Teaching Project, Benchmark Portfolio, Kaustav Majumder

UNL Faculty Course Portfolios

FDST 880P: Food Proteins, is a course designed only for the graduate students for the department of food science and technology. The course work provides the opportunity to dwell deep into the fundamental chemistry, protein structures and functions to understand the physiochemical and biochemicals factors that govern the functionality and biological activity of the food proteins and proteins-derived peptides. For each of the course objectives, I describe how and why I organize the course, as well as how my pedagogical choices encourage the student learning and engagement. Collect and analyze the data on students' performance and progress during the semester …


Critical Thinking, Perspective-Taking, And Cultural Humility In Global Refugee Health Studies: A Peer Review Of Teaching Benchmark Portfolio, Julie A. Tippens Jan 2020

Critical Thinking, Perspective-Taking, And Cultural Humility In Global Refugee Health Studies: A Peer Review Of Teaching Benchmark Portfolio, Julie A. Tippens

UNL Faculty Course Portfolios

This portfolio examines the third iteration of a course I developed for the Department of Child, Youth and Family Studies: CYAF 493 – Global Case Studies in Refugee Health and Wellbeing. The primary goal of this course is for students to think critically about the social and structural determinants of refugee health and wellbeing. I have also integrated three core course values to guide instruction: critical thinking, perspective-taking, and cultural humility. Student learning and engagement was analyzed through four areas: (1) a baseline assessment of students’ perceptions of refugees, (2) weekly homework reflections, (3) a book review assignment, and (4) …


Nutritional Strategies For Nurses In A Fast Paced Occupation, Zachary Christman Jan 2020

Nutritional Strategies For Nurses In A Fast Paced Occupation, Zachary Christman

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

The foundation of the medical system are the nurses and certified medical assistants. Their personal health condition affects how well diseases and other medical conditions are handled. This article will begin with an overview of the eating behavior of nurses. Three different interventions which have been shown effective in a fast paced workplace are presented in order to improve the health of nurses and certified medical assistants. The first intervention is organizational changes that would increase the amount of time nursing staff have to eat. The second is the use of a messenger bag delivery system to provide needed food, …