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Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor Jan 1998

Cytoskeletal Association Of An Esterase In Dictyostelium Discoideum, Catherine P. Chia, Lothar Bomblies, Kristy K. Taylor

Papers in Microbiology

A 70-kDa glycoprotein, gp70, was found enriched in the detergentinsoluble cytoskeletal fraction of axenically grown Dictyostelium discoideum cells. Its N-terminal amino acid sequence identified it as “crystal protein” (L. Bomblies et al., 1990, J. Cell Biol. 110, 669– 679). This finding was corroborated when antibody to crystal protein cross-reacted with gp70 and its deglycosylated form. The postulated esterase activity of gp70/crystal protein was verified through comparative enzyme assays of extracts derived from cells that either overexpressed or lacked gp70. Gp70 cosedimented with cytoskeletons on sucrose gradients, suggesting an interaction with the cytoskeleton. Coisolation of gp70 with detergent-extracted cells, observed by …


Carnival And Ceremony At Wright Morris's Lone Tree Hotel, Reginald Dyck Jan 1998

Carnival And Ceremony At Wright Morris's Lone Tree Hotel, Reginald Dyck

Great Plains Quarterly (through 2013)

Gordon Boyd, a central character in Morris's Ceremony in Lone Tree, is a self-fashioning failure at American dreaming. He presents himself this way: "In the middle of life, Morgenstern Boyd had everything to live for, everything worth living for having eluded him. He was that rare thing, a completely selfunmade man." Failed writer among other failures, Boyd in many ways contrasts with, and is defined by, his friend and nemesis Walter McKee, a man who "like Babbitt, keeps faith; he does not question anything, especially his own life. " Set in the 1950s Midwest culture of respectability, Ceremony in …


Review Of Black Valor: Buffalo Soldiers And The Medal Of Honor, 1870-1898 By Frank N. Schubert, Cornel Pewewardy Jan 1998

Review Of Black Valor: Buffalo Soldiers And The Medal Of Honor, 1870-1898 By Frank N. Schubert, Cornel Pewewardy

Great Plains Quarterly (through 2013)

Copyright 1998 by the Center for Great Plains Studies, University of Nebraska- Lincoln


Review Of A Force Upon The Plain: The American Militia Movement And The Politics Of Hate. By Kenneth S. Stern, Catherine Mcnicol-Stock Jan 1998

Review Of A Force Upon The Plain: The American Militia Movement And The Politics Of Hate. By Kenneth S. Stern, Catherine Mcnicol-Stock

Great Plains Quarterly (through 2013)

Nine days before the 19 April 1995 bombing of the Alfred P. Murrah Federal Building in Oklahoma City, Kenneth Stern, an expert on hate groups for the American Jewish Committee (AJC), warned of just such an attack. In a Washington, D.C., press conference, Stern and other AJC professionals presented a six hundred page report entitled Militias: A Growing Danger warning of the "cauldron of disaffection, hate, conspiracy and violence brewing" around the country. They documented murders of federal workers and "anyone perceived as opposing the militia and therefore seen as doing 'the work of the government.'" Moreover, they suggested that …


Faculty Development In Technology Applications To University Instruction: An Evaluation, Margie K. Kitano, Bernard J. Dodge, Patrick J. Harrison, Rena B. Lewis Jan 1998

Faculty Development In Technology Applications To University Instruction: An Evaluation, Margie K. Kitano, Bernard J. Dodge, Patrick J. Harrison, Rena B. Lewis

To Improve the Academy: A Journal of Educational Development

Progress in integrating new technologies into higher education classrooms has been slow despite emerging evidence on benefits for students when technologies are applied in ways that support teaching and learning. This article describes a program used by a college of education to support faculty applications of technology in instruction and reports results of a formal evaluation following the first year of implementation. The program provided intensive training and follow-up support to a heterogeneous cohort of 14 faculty members and was designed to enhance their ability to integrate technology into their teaching, use a new "smart" classroom facility, and/or develop products …


A Case Study In Getting Faculty To Change, Joan K. Middendorf Jan 1998

A Case Study In Getting Faculty To Change, Joan K. Middendorf

To Improve the Academy: A Journal of Educational Development

Academic support professionals have a lot to share with faculty, but it is our special challenge that faculty do not always welcome our help. We can achieve greater success and suffer less frustration by understanding some principles about the process of change. This article offers four principles of implementing change and illustrates their application to a project. If academic support professionals prepare to offset resistance, model a vision of success, involve key people, and match strategies to the stages faculty move through in accepting a change, we can enhance adoption of new approaches.


Chapter 2 Drought Planning In The United States: Status And Future Directions, Donald A. Wilhite, Michael J. Hayes Jan 1998

Chapter 2 Drought Planning In The United States: Status And Future Directions, Donald A. Wilhite, Michael J. Hayes

National Drought Mitigation Center: Faculty Publications

Drought has been a common feature in large portions of the United States during the past decade. To the casual observer, the frequency of recent droughts may signal a change in climate, a possible forewarning of global warming. Viewed in the context of the nation’s climatic history, the pattern of the past decade is not unusual.


Ec98-802 Soybean Basis Patterns From Selected Sites In Nebraska Jan 1998

Ec98-802 Soybean Basis Patterns From Selected Sites In Nebraska

University of Nebraska-Lincoln Extension: Historical Materials

The following publication contains soybean basis patterns for several towns in Nebraska. The basic price information was collected through surveys, newspaper, electronic media, etc. The listing includes towns that are representative of different geographic locations in Nebraska. The amount of data varies among locations. This publication will be updated each year by adding a year's data to each location which will allow the user to observe the changes in the basis patterns over time.


Ec98-898 Wheat Basis Patterns From Selected Sites In Nebraska Jan 1998

Ec98-898 Wheat Basis Patterns From Selected Sites In Nebraska

University of Nebraska-Lincoln Extension: Historical Materials

The following publication contains wheat basis patterns for several towns in Nebraska. The basic price information was collected through surveys, newspaper, electronic media, etc. The listing includes towns that are representative of different geographic locations in Nebraska. The amount of data varies among locations. This publication will be updated each year by adding a year's data to each location which will allow the user to observe the changes in the basis patterns over time.


The Probe, Issue 184 – January 1998 Jan 1998

The Probe, Issue 184 – January 1998

The Probe: Newsletter of the National Animal Damage Control Association

NADCA Election Results!
PRESIDENT Robert H. Giles, Jr. (VA) VICE PRESIDENT - WEST Mark Collinge (ID) VICE PRESIDENT - EAST Pete Butchko (MS) SECRETARY Richard B. Chipman (VT) TREASURER Grant Huggins (OK)
REGIONAL DIRECTORS: Western (1): Diane deLorimier (CA) Southern Rockies (2): GaryWitmer (CO) Northern Rockies (3): George E. Graves (ID) South Central (4): James R. Gallaspy (LA) North Central (5): James C. Luchsinger (NE) Great Lakes (6): Mike Dwyer (OH) Northeastern (7): Jerry L. Pickel (PA) Centraleastern (8): JohnM. Houben (VA) Southeastern (9): D. Tommy King (MS)
From Our Incoming President: An Opinion....-- Robert H. Giles, Jr.
Carrie Hunt …


Impact Of Temperature And Humidity On Host-Pathogen Interactions Between Beauveria Bassiana And A Coccinellid, R. R. James, B. A. Croft, B. T. Shaffer, B. Lighthart Jan 1998

Impact Of Temperature And Humidity On Host-Pathogen Interactions Between Beauveria Bassiana And A Coccinellid, R. R. James, B. A. Croft, B. T. Shaffer, B. Lighthart

Entomology Papers from Other Sources

We tested the effect of 2 exposure methods and 2 environmental factors, temperature and humidity, on Beauceria bassiana (Deuteromycotina: Hyphomycetes) mycosis in Hippodamia convergens Guerin-Meneville (Coleoptera: Coccinellidae). We found that the standard EPA bioassay method of exposing nontarget insects by immersion resulted in a per-insect dose that was 5 times greater than that from spray applications. However, this difference did not significantly affect mycosis levels in a simulation of field applications conducted in the greenhouse. In contrast, differences in environmental conditions between the laboratory and greenhouse had a large effect on mycosis. Constant temperatures between 15 and 35°C had a …


Expression Of An Inter-Α-Trypsininhibitor Heavy Chain-Like Protein In The Pig Endometrium During The Oestrous Cycle And Early Pregnancy, R. D. Geisert, J. V. Yelich, T. Pratt, Daniel Pomp Jan 1998

Expression Of An Inter-Α-Trypsininhibitor Heavy Chain-Like Protein In The Pig Endometrium During The Oestrous Cycle And Early Pregnancy, R. D. Geisert, J. V. Yelich, T. Pratt, Daniel Pomp

Department of Animal Science: Faculty Publications

In pigs, changes in an unidentified endometrial glycoprotein, pGP30, are temporally associated with rapid trophoblast elongation and initial placental attachment on day 12 of gestation. Identification of endometrial pGP30 was undertaken through protein purification, NH2-terminal amino acid sequencing and cDNA sequencing of products generated through reverse transcription-polymerasechain reaction. Sequencing of 35 amino acids from the NH2-terminal end of pGP30 revealed that the 30 kDa glycoprotein is a cleavage product from the C-terminal region of inter-α-trypsininhibitor heavy chain 4 (IαIH4), previously known as inter-α-trypsininhibitor heavy chain-like protein. IαIH4 is unique compared with the three other inter-α-trypsininhibitor heavy chains as it does …


The Moderate Resolution Imaging Spectroradiometer (Modis): Land Remote Sensing For Global Change Research, Christopher O. Justice, Eric Vermote, John R. G. Townshend, Ruth Defries, David P. Roy, Dorothy K. Hall, Vincent V. Salomonson, Jeffrey L. Privette, George Riggs, Alan Strahler, Wolfgang Lucht, Ranga P. Myneni, Yuri Knyazikhin, Steve W. Running, Rama R. Nemani, Zhengming Wan, Alfredo R. Huete, Wim Van Leeuwen, Robert E. Wolfe, Louis Giglio, Jan-Peter Muller, Philip Lewis, Michael J. Barnsley Jan 1998

The Moderate Resolution Imaging Spectroradiometer (Modis): Land Remote Sensing For Global Change Research, Christopher O. Justice, Eric Vermote, John R. G. Townshend, Ruth Defries, David P. Roy, Dorothy K. Hall, Vincent V. Salomonson, Jeffrey L. Privette, George Riggs, Alan Strahler, Wolfgang Lucht, Ranga P. Myneni, Yuri Knyazikhin, Steve W. Running, Rama R. Nemani, Zhengming Wan, Alfredo R. Huete, Wim Van Leeuwen, Robert E. Wolfe, Louis Giglio, Jan-Peter Muller, Philip Lewis, Michael J. Barnsley

United States National Aeronautics and Space Administration: Publications

The first Moderate Resolution Imaging Spectroradiometer (MODIS) instrument is planned for launch by NASA in 1998. This instrument will provide a new and improved capability for terrestrial satellite remote sensing aimed at meeting the needs of global change research. The MODIS standard products will provide new and improved tools for moderate resolution land surface monitoring. These higher order data products have been designed to remove the burden of certain common types of data processing from the user community and meet the more general needs of global-to-regional monitoring, modeling, and assessment. The near-daily coverage of moderate resolution data from MODIS, coupled …


Influence Of Iron Oxide Inclusion Shape On Coii/Iiiedta Reactive Transport Through Spatially Heterogeneous Sediment, James E. Szecsody, Ashley Chilakapati, John M. Zachara, Amanda Garvin Jan 1998

Influence Of Iron Oxide Inclusion Shape On Coii/Iiiedta Reactive Transport Through Spatially Heterogeneous Sediment, James E. Szecsody, Ashley Chilakapati, John M. Zachara, Amanda Garvin

United States Department of Energy: Publications

Multisolute reactive transport was investigated in chemically heterogeneous systems to determine the influence of the shapes of the reactive heterogeneities (iron oxide inclusions) by comparison of two-dimensional heterogeneous experiments with spatially averaged models that had differing inclusion characterization. Eleven reactions were considered in this system, starting with absorption of the initial solute (CoIIEDTA) to Fe oxides, followed by two competing surface reactions: oxidation forming CoIIIEDTA and Fe dissolution forming FeIIIEDTA and Co2+. Spatial moments of the eight mobile species were compared between data and models. One spatially averaged model (homogenous equivalent), which incorporated inclusion …


Bacterial Reduction Of Crystalline Fe3+ Oxides In Single Phase Suspensions And Subsurface Materials, John M. Zachara, James K. Fredrickson, Shu-Mei Li, David W. Kennedy, Steven C. Smith, Paul L. Gassman Jan 1998

Bacterial Reduction Of Crystalline Fe3+ Oxides In Single Phase Suspensions And Subsurface Materials, John M. Zachara, James K. Fredrickson, Shu-Mei Li, David W. Kennedy, Steven C. Smith, Paul L. Gassman

United States Department of Energy: Publications

Microbiologic reduction of synthetic and geologic Fe3+ oxides associated with four Pleistocene- age, Atlantic coastal plain sediments was investigated using a dissimilatory Fe reducing bacterium (Shewanella putrefaciens, strain CN32) in bicarbonate buffer. Experiments investigated whether phosphate and anthraquinone-2, 6-disulfonate, (AQDS, a humic acid analogue) influenced the extent of crystalline Fe3+ oxide bioreduction and whether crystalline Fe3+ oxides in geologic materials are more or less reducible than comparable synthetic phases. Anaerobic incubations (108 organisms/mL) were performed both with and without PO4 and AQDS that functions as an electron repository and shuttle. The production of …


1998 Platte River Basin Ecosystem Symposium Proceedings Jan 1998

1998 Platte River Basin Ecosystem Symposium Proceedings

Water Current Newsletter

No abstract provided.


Tracing Intricate Thread Control Systems: The Bamboo Drum Or Swine Basket Loom To The Tc-L, Carol D. Westfall Jan 1998

Tracing Intricate Thread Control Systems: The Bamboo Drum Or Swine Basket Loom To The Tc-L, Carol D. Westfall

Textile Society of America: Symposium Proceedings

I use the word "primitive" with great care and caution and a certain humility for we now send men and women into space clad in triaxially woven fabrics which stem from a basic hexagonal basket structure perfected eons ago by some "primitive" Chinese artisan. I am consistently amazed by the extraordinary accomplishments of our common ancestors.

Man's need to adorn his or her body with imagery which acts as a signifier of status or aesthetic is a time honored tradition. The transference of this imagery onto textiles was a natural occurrence, once the need for garments was established - either …


Development Of Banded Sunflower Moth (Lepidoptera: Cochylidae) And Sunflower Moth (Lepidoptera: Pyralidae) On Three Diets, Jawahar L. Jyoti, Gary J. Brewer Jan 1998

Development Of Banded Sunflower Moth (Lepidoptera: Cochylidae) And Sunflower Moth (Lepidoptera: Pyralidae) On Three Diets, Jawahar L. Jyoti, Gary J. Brewer

Department of Entomology: Faculty Publications

A comparative study of the effects of sunflower moth diet, banded sunflower moth diet, and a modified sunflower moth diet was conducted on banded sunflower moth, Cochylis hospes Walsingham, and sunflower moth, Homoeosoma electellum (Hulst). Developmental times and survival to the pupal and adult stages were measured for each diet and pest species. Modified sunflower moth diet with incorporated sunflower leaf tissue gave faster developmental times and a higher percent pupation and adult eclosion for both sunflower moth species than either sunflower moth diet or banded sunflower moth diet. The modified sunflower moth diet that incorporates sunflower leaf tissue provides …


Generations Of Families: A Teaching Project And Photograph Exhibition, Carolyn P. Edwards, Kay Springate Jan 1998

Generations Of Families: A Teaching Project And Photograph Exhibition, Carolyn P. Edwards, Kay Springate

Department of Child, Youth, and Family Studies: Faculty Publications

"Generations of Families" was a collaborative project involving university and community partners. University of Kentucky students worked with public and private local agencies, leaders, and citizens to collect, catalog, label, and display treasured family photographs of local citizens. The final product consisted of five separate displays totaling hundreds of framed or mounted photographs. The project supported the development of cross-cultural competence and professional skills by Family and Consumer Sciences students. The project achieved significant impact, as measured by community response, exit interviews, and analysis of student journals.


A New Method Of Retrieving Surface Bidirectional Reflectance From Ground Measurements: Atmospheric Sensitivity Study, A. I. Lyapustin, J. L. Privette Jan 1998

A New Method Of Retrieving Surface Bidirectional Reflectance From Ground Measurements: Atmospheric Sensitivity Study, A. I. Lyapustin, J. L. Privette

United States National Aeronautics and Space Administration: Publications

Commonly, experimental data on surface directional reflectance are not properly corrected for atmospheric effects. In this paper, we describe a new method for rigorous retrieval of the bidirectional reflectance distribution function (BRDF) from ground-based measurements.


Susceptibility Of House Fly (Diptera: Muscidae) Larvae To Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae, Steinernematidae), David B. Taylor, Allen L. Szalanski, Byron J. Adams, Richard D. Peterson Ii Jan 1998

Susceptibility Of House Fly (Diptera: Muscidae) Larvae To Entomopathogenic Nematodes (Rhabditida: Heterorhabditidae, Steinernematidae), David B. Taylor, Allen L. Szalanski, Byron J. Adams, Richard D. Peterson Ii

Department of Entomology: Faculty Publications

The potential for entomopathogenic nematodes to control flies in cattle feedlots was determined by screening 40 strains representing 8 species of Heterorhabditis Poinar and 5 species of Steinemema Travassos for virulence toward 3rd-instar house flies (maggots), Musca domestica L. None of the 22 strains of Heterorhabditis infecting maggots caused significant levels of mortality in a filter paper assay. Ten strains of Steinemema infected maggots, of which 7 strains (4 S. carpocapsae (Weiser), 2 S.feltiae (Filipjev), and 1 S. scapterisci Nguyen & Smart) caused significant mortality. Ten Heterorhabditis strains and 10 Steinemema strains successfully reproduced for ≥ 2 generations in maggots. …


Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark Jan 1998

Growth Traits Among Sorghum Genotypes In Response To Aluminum, J. H. Bernal, R. B. Clark

Department of Agronomy and Horticulture: Faculty Publications

Aluminum (Al) has many detrimental effects on plant growth, and shoots and roots are normally affected differently. A study was conducted to determine differences among sorghum [Sorghum bicolor (L.) Moench] genotypes with broad genetic backgrounds for growth traits of plants grown at 0,200,400,600, and 800 uM Al in nutrient solutions (pH 4.0). Genotypes were categorized into "Al-sensitive", "intermediate Al-tolerant", "Al-tolerant", and SC 283 (an Al-tolerant standard). As Al increased, shoot and root dry matter (DM), net main axis root length (NMARL), and total root length (TRL) became lower than controls (0 Al). Aluminum toxicity and/or nutrient deficiency symptoms become …


Matrix-Enhanced Calibration Procedure For Multivariate Calibration Models With Near-Infrared Spectra, Mark R. Riley, Mark A. Arnold, David W. Murhammer Jan 1998

Matrix-Enhanced Calibration Procedure For Multivariate Calibration Models With Near-Infrared Spectra, Mark R. Riley, Mark A. Arnold, David W. Murhammer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

A novel method is introduced for developing calibration models for the spectroscopic measurement of chemical concentrations in an aqueous environment. To demonstrate this matrix-enhanced calibration procedure, we developed calibration models to quantitate glucose and glutamine concentrations in an insect cell culture medium that is a complex mixture of more than 20 components, with three components that manifest significant concentration changes. Accurate calibration models were generated for glucose and glutamine by using a calibration data set composed of 60 samples containing the analytes dissolved in an aqueous buffer along with as few as two samples of the analytes dissolved in culture …


Purification And Characterization Of A Soybean Root Nodule Phosphatase Expressed In Pichia Pastoris, Alan R. Penheiter, Robert V. Klucas, Gautam Sarath Jan 1998

Purification And Characterization Of A Soybean Root Nodule Phosphatase Expressed In Pichia Pastoris, Alan R. Penheiter, Robert V. Klucas, Gautam Sarath

Gautam Sarath Publications

Soybean root nodules possess a developmentally regulated acid phosphatase (ACP) that exhibits the highest specificity for purine 5’-nucleoside monophosphates. The enzyme is a glycosylated dimer of 28- and 31-kDa subunits, which appear to be products of the same gene but differ in posttranslational modifications. In order to perform directed mutagenesis and more extensive biochemical characterization, a means of producing recombinant ACP was needed. Several attempts were made to express ACP in Escherichia coli, but all conditions employed resulted in protein that was found entirely in inclusion bodies, and resolubilization experiments were unsuccessful. Therefore, the methyltrophic yeast Pichia pastoris was …


Holistic Faculty Development: Supporting Personal, Professional, And Organizational Well-Being, Glenda T. Hubbard, Sally S. Atkins, Kathleen T. Brinko Jan 1998

Holistic Faculty Development: Supporting Personal, Professional, And Organizational Well-Being, Glenda T. Hubbard, Sally S. Atkins, Kathleen T. Brinko

To Improve the Academy: A Journal of Educational Development

In recent years, higher education has begun to realize the great influence that faculty quality of life has on student learning and on overall institutional effectiveness. This article examines the interactive effect of personal, professional, and organizational well-being and describes a center that integrates four kinds of services-faculty development, employee assistance, health promotion, and organizational development-that work both separately and collaboratively. The result is a synergistic organization that is able to tackle complex institutional problems that could not be addressed by any one program alone.


Minimizing Error When Developing Questionnaires, Terrie Nolinske Jan 1998

Minimizing Error When Developing Questionnaires, Terrie Nolinske

To Improve the Academy: A Journal of Educational Development

Questionnaires are used by faculty developers, administrators, faculty, and students in higher education to assess need, conduct research, and evaluate teaching or learning. While used often, questionnaires may be the most misused method of collecting information, due to the potential for sampling error and nonsampling error, which includes questionnaire design, sample selection, nonresponse, wording, social desirability, recall, format, order, and context effects. This article offers methods and strategies to minimize these errors during questionnaire development, discusses the importance of pilot-testing questionnaires, and underscores the importance of an ethical approach to the process. Examples relevant to higher education illustrate key points.


Review Of Native Americans In The News: Images Of Indians In The Twentieth Century Press By Mary Ann Weston, Gary Garrison Jan 1998

Review Of Native Americans In The News: Images Of Indians In The Twentieth Century Press By Mary Ann Weston, Gary Garrison

Great Plains Quarterly (through 2013)

As a lay historian of the American Indian and a television producer specializing in programs dealing with Native American subject matter, I've lectured frequently at high schools and colleges on stereotypical images of Native Americans in film, television, and popular literature and on how these media have shaped and reinforced such images throughout our turbulent history of contact. Focusing only on entertainment in the past, I found Native Americans in the News: Images of Indians in the Twentieth Century Press singularly informative in how the news media have also molded and solidified the general public's perceptions of Native Americans.

Mary …


Review Of The Hog Ranches Of Wyoming: Liquor, Lust, And Lies Under Sagebrush Skies By Larry K. Brown, Rebecca Howard Jan 1998

Review Of The Hog Ranches Of Wyoming: Liquor, Lust, And Lies Under Sagebrush Skies By Larry K. Brown, Rebecca Howard

Great Plains Quarterly (through 2013)

In The Best Little Whorehouse in Texas by Larry L. King and Peter Masterson, the titled place of ill-repute was nicknamed the Chicken Ranch. In Wyoming locals referred to such places as Hog Ranches. Established to supply wine, women, and song to military outposts in the nineteenth century, the history of the Hog Ranches has received little attention until now. It is the story of these dens which Larry K. Brown pursues here. The book has the cover of a dime store novel and a title that draws immediate attention. For these reasons readers might approach the work looking for …


Substituted Polythiophenes From Highly Reactive Zinc Reagents, Reuben D. Rieke Jan 1998

Substituted Polythiophenes From Highly Reactive Zinc Reagents, Reuben D. Rieke

Department of Chemistry: Faculty Publications

A novel Zerovalent zinc species and an organozinc reagent are disclosed. The zerovalent zinc species is directly pro duced by reaction of a reducing agent on a zinc salt, preferably Zn(CN)2. The organozinc reagent results from the reaction of the zerovalent zinc species and an organic compound having one or more stable anionic leaving groups. These organozinc reagents include a wide spectrum of functional groups in the organic radical, and are useful in a variety of reactions schemes.


Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna Jan 1998

Selective And Organotypic Culture Of Intrahepatic Bile Duct Cells From Adult Pig Liver, Neil C. Talbot, Thomas J. Caperna

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Secondary culture of nontransformed bile duct epithelium has been difficult to achieve. STO feeder cell-dependent secondary cultures of adult pig bile duct cells were established from primary cultures of adult pig liver cells. Adult pig hepatocytes exhibited limited or no replication and were lost from the secondary culture at Passage 3 or 4. In contrast, adult pig bile duct cells replicated and were carried for 4-8 passages in secondary culture. A simple method to produce nearly pure pig intrahepatic bile duct cultures was first to freeze a relatively crude liver cell preparation. Upon subsequent thawing, all hepatocytes and most macrophages …