Digital Commons Network^™

Solving Human Factor Issues As Applied To The Work Force, Robert Miles, Dennis Attwood, Amy White

United States Department of Transportation: Publications

This paper aims to capture a range of current approaches to the management and delivery of safety by means of addressing workforce human factors issues. The paper is divided into a number of sections and each section is the product of the facilitated debate and discussions around the original discussion paper.

Gemfibrozil, A Lipid-Lowering Drug, Inhibits The Induction Of Nitric-Oxide Synthase In Human Astrocytes, Kalipada Pahan, Malabendu Jana, Xiaojuan Liu, Bradley S. Taylor, Charles Wood, Susan M. Fischer

Nebraska Center for Virology: Faculty Publications

Gemfibrozil, a lipid-lowering drug, inhibited cytokine- induced production of NO and the expression of inducible nitric-oxide synthase (iNOS) in human U373MG astroglial cells and primary astrocytes. Similar to gemfibrozil, clofibrate, another fibrate drug, also inhibited the expression of iNOS. Inhibition of human iNOS promoter-driven luciferase activity by gemfibrozil in cytokine-stimulated U373MG astroglial cells suggests that this compound inhibits the transcription of iNOS. Since gemfibrozil is known to activate peroxisome proliferator- activated receptor-α (PPAR-α), we investigated the role of PPAR- α in gemfibrozil-mediated inhibition of iNOS. Gemfibrozil induced peroxisome proliferator-responsive element (PPRE)-dependent luciferase activity, which was inhibited by the expression of …

Derivation Of Risk Based Wipe Surface Screening Levels For Industrial Scenarios, Lisa M. May, Bonnie Gaborek, Tony Pitrat, Laura Peters

Uniformed Services University of the Health Sciences

The environmental characterization of building interiors and other surfaces has generally been performed with wipe-sampling because it is a non-destructive technique. There is no consensus, however, as to the interpretation of the results of wipe-sampling. Specifically, there is not a standardized method to determine if chemicals found at sampled levels pose a threat to human health. A methodology was developed, based on acceptable health risk levels, to derive screening levels for evaluating wipe-sampling results pertaining to industrial scenarios. The methodology was based on the United States Environmental Protection Agency (USEPA) Region IX Preliminary Remediation Goal (PRG) approach; a multi-exposure methodology …

Peacock Alley: Highway 41 And The Growth Of The Chenille Bedspread Industry, Ashley Callahan

Textile Society of America: Symposium Proceedings

In the fall of 1935. a newspaper editor traveled from Oklahoma City to Atlanta to attend a baseball game, and along the way encountered a stretch of road near Dalton. Georgia known as Bedspread Boulevard. He recorded his experiences in his daily column: "Twisting through northern Georgia late Saturday afternoon, dodging cotton wagons and trying to get an eyeful of the gorgeous tints that glorified the turning trees in the mountains, I thought I saw a washing strung on a line by the roadside. Soon another flashed past. Then they followed in regular succession. . . . Is it possible …

Targeting Outcomes Of Programs (Top): A Hierarchy For Targeting Outcomes And Evaluating Their Achievement (Powerpoint Presentation), S. Kay Rockwell

Department of Agricultural Leadership, Education, and Communication: Faculty Publications

Why Assess needs and evaluate?
1.Help establish goals and objectives
2.Test if met goals and objectives
3.Identify outcomes/impact on..
Individuals
Community
4.Provide documentation for stakeholders
5.Document accountability

The "Document" (at right) is a PDF version of the PowerPoint slides. The slides are attached (below) as an additional .ppt file

Killing Of Mycobacterium Avium Subspecies Paratuberculosis Within Macrophages, John P. Bannantine, Judith R. Stabel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Mycobacterium avium subspecies paratuberculosis (M. paratuberculosis) is a facultative intracellular pathogen that resides within host macrophages during infection of ruminant animals. We examined survival of M. paratuberculosis infections within cultured macrophages to better understand the interplay between bacterium and host.

Results: Serial plating of M. paratuberculosis infected macrophage lysates on Herold's egg yolk medium showed that mycobacterial replication takes place between 0 and 24 hours post-infection. This initial growth phase was followed by a steady decline in viability over the next six days. Antibodies against M. paratuberculosis were affinity purified and used in conjunction with transmission electron microscopy to …

Growing Rice Grain With Controlled Cadmium Concentrations, Urszula Kukier, Rufus L. Chaney

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two solution studies were conducted a) to investigate the uptake of zinc (Zn) and cadmium (Cd) by rice plants (Oryza sativa L.) and interaction between these elements, and b) to determine experimental conditions for growing rice grain with desired Cd concentration for an animal feeding study. In both studies, free metal activities of cadmium and cationic microelements were buffered by an excess of chelating agents. The first study was a factorial design with two Zn levels (1.0 and 3.89 μM) and four Cd levels (0.81, 1.44, 2.56 and 4.55 μM) in the solution. In the second study, rice was …

A Personal Account Of Creating The Sterile Insect Technique To Eradicate The Screwworm From Curacao, Florida And The Southeastern United States, Alfred H. Baumhover

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The history is recounted of developing the sterile insect technique to eradicate the screwworm, Cochliomyia hominivorax (Coquerel), from the Caribbean island of Curacao, Florida and the southeastern U.S. Observations of screwworm biology and challenges faced in conducting these eradication projects are described by the author who worked on all aspects of the research and field operations. Eradication was first demonstrated on Curacao, essentially a 170 mi² outdoor laboratory. The population dynamics of the wild screwworm was determined and overflooding ratios and dispersal patterns essential for population suppression were defined. Eradication was achieved with minimal resources by attacking the pest …

Differences Among Mycorrhizal Fungi For Mineral Uptake Per Root Length Of Switchgrass Grown In Acidic Soil, R. B. Clark

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plants commonly encounter deficient and=or toxic levels of mineral elements when grown in acidic soil, and arbuscular mycorrhizal fungi (AMF) have potential to overcome many of these effects. Switchgrass (Panicum virgatum L.) was grown in acidic Lily soil (Typic Hapludult) at pH_Ca 4 and 5 (soil : 10mM CaCl₂, 1 : 1) and inoculated with Glomus (G.) clarum, G. diaphanum, G. etunicatum, G. intraradices, Gigaspora (Gi.) albida, Gi. margarita, Gi. rosea, and Acaulospora (A.) morrowiae to determine differences among mycorrhizal (AM) plants for mineral uptake per root length (RL). Shoots of AM plants grown …

Genomewide Identification Of Proteins Secreted By The Hrp Type Iii Protein Secretion System Of Pseudomonas Syringae Pv. Tomato Dc3000, Tanja Petnicki-Ocwieja, David J. Schneider, Vincent C. Tam, Scott T. Chancey, Libo Shan, Yashitola Jamir, Lisa M. Schechter, Misty Janes, C. Robin Buell, Xiaoyan Tang, Alan Collmer, James R. Alfano

Department of Plant Pathology: Faculty Publications

The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends on the Hrp (hypersensitive response and pathogenicity) type III protein secretion system and the effector proteins it translocates into plant cells. Through iterative application of experimental and computational techniques, the DC3000 effector inventory has been substantially enlarged. Five homologs of known avirulence (Avr) proteins and five effector candidates, encoded by genes with putative Hrp promoters and signatures of horizontal acquisition, were demonstrated to be secreted in culture and/or translocated into Arabidopsis in a Hrp-dependent manner. These 10 Hrp-dependent outer proteins (Hops) were designated HopPtoC (Avr- PpiC2 …

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca²⁺-independent and is sensitive to Cl^– channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are …

Parelaphostrongylus Tenuis In Captive Pronghorn Antelope (Antilocapra Americana) In Nebraska, Heather A. Simmons, David J. Steffen, Douglas L. Armstrong, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

Lesions in four captive pronghorn antelope (Antilocapra americana) naturally infected with Parelaphostrongylus tenuis in eastern Nebraska (USA) are described in this report. Animals were bright and alert with hind limb ataxia that progressed to sternal or lateral recumbency between July 28 and October 17, 1998. Animals were euthanized due to disease progression despite therapy. Multifocal decubital ulcers over bony prominences occurred in two animals and chronic unilateral otitis media was present in one animal. Histopathologic examination revealed severe Wallerian degeneration randomly scattered throughout the spinal cords of all four animals. Spinal cord sections from two animals contained adult …

A Physically Based Two-Dimensional Infiltration Model For Furrow Irrigation, Christopher J. Skonard, Derrel L. Martin

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A two-dimensional physically based infiltration model was developed for furrow
irrigation. Infiltration was simulated using the Green-Ampt infiltration method. The Green-Ampt infiltration parameters are available from numerous sources, unlike the Kostiakov infiltration parameters. Simulation tests showed the two-dimensional model capable of estimating cumulative infiltration volume within 8% compared to simulated infiltration using the finite element model, Hydrus-2D. Application of the two-dimensional model in a surface irrigation advance model allows irrigation performance parameters to be predicted without extensive soil experiments.

Kinetic Properties Of Bifunctional 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase From Spinach Leaves, Jennifer E. Markham, Nicholas J. Kruger

Department of Biochemistry: Faculty Publications

A cDNA encoding 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase was isolated from a Spinacia oleracea leaf library and used to ex­press a recombinant enzyme in Escherichia coli and Spodoptera frugiperda cells. The insoluble protein expressed in E. coli was purified and used to raise antibodies. Western blot analysis of a protein extract from spinach leaf showed a single band of 90.8 kDa. Soluble protein was purified to homogeneity from S. frugiperda cells infected with recombinant baculovirus harboring the isolated cDNA. The soluble protein had a molecular mass of 320 kDa, estimated by gel filtration chromatography, and a subunit size of 90.8 kDa. The purified pro­tein …

A Preliminary Analysis Of Proteolytic Activity Of Excretory-Secretory Products From Cyathostominea, Jane L. Kinsella, J. Ralph Lichtenfels, Michael F. Ryan

Harold W. Manter Laboratory of Parasitology: Faculty Publications

The excretory-secretory product (ESP) derived from Cyathostominea in vitro was assessed, in terms of subunit composition, and proteolytic activity using as substrates azocasein and two synthetic fluorogenic peptides. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) resolved 13 subunits, and the presence of the protein cysteine proteinase activator dithiothreitol (DTT) revealed 21 subunits.DTTalso enhanced azocaseinolysis, and hydrolysis of carbobenzoxy-phenylalanyl-arginine-7-amido-4-methylcoumarin (Z-Phe-Arg-NHMec) and carbobenzoxy-arginyl-arginine-7-amido-4-methylcoumarin (Z-Arg-Arg-NHMec). At the optimum pH of 5.5, hydrolysis of Z-Phe-Arg-NHMec was three-fold greater than that of Z-Arg-Arg-NHMec suggesting that the proteolytic specificities of the ESP are more like those of papain or cathepsin L, rather than cathepsin B. In SDS-PAGE …

Development Of Hydrotaea Aenescens (Diptera: Muscidae) In Manure Of Unweaned Dairy Calves And Lactating Cows, Jerome Hogsette, Robert Farkas, Reginald Coler

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

In laboratory studies performed in the United States and Hungary, the dump fly Hydrotaea aenescens (Wiedemann) was reared successfully in manure of 1- to 8-wk-old dairy calves, and in manure from adult lactating dairy cows. Survival in manure collected from 1-wk-old calves was poor (7.2%), better in manure collected from 2- and 3-wk-old calves (53.5%), and best in manure collected from 4- to 8-wk-old calves (71.4%). Survival in cow manure was slightly lower (47.4%) than that in calf manure. Reasons for different rates of development in the United States and in Hungary, and by calf age are discussed as are …

Germination Of Threadleaf Sedge (Cyperaceae: Carex Filifolia), Gina R. Tichota, James L. Stubbendieck

Transactions of the Nebraska Academy of Sciences and Affiliated Societies

Threadleaf sedge (Carex filifolia Nutt.) is one ofthe most important dryland plants native to the grasslands of the northern Great Plains. Low seed production and poor germination are primary reasons making prairie restorations that include this species a challenge. The principal objective of this research was to investigate the influences of warm and cold pretreatments, moisture regime, and substrate type on germination. The work was conducted in a germination chamber with seeds (achenes) collected at two sites and in two years in western Nebraska. Average germination was relatively low with the common pretreatments of cold-wet alone (39% in 1998 …

A New Flavor-Coated Sachet Bait For Delivering Oral Rabies Vaccine To Raccoons And Coyotes, Samuel B. Linhart, John C. Wlodkowski, Darrell M. Kavanaugh, Laurie Motes-Kreimeyer, Andrew J. Montoney, Richard B. Chipman, Dennis Slate, Laura L. Bigler, Malcomb G. Fearneyhough

United States Department of Agriculture Wildlife Services: Staff Publications

Research was conducted during 1996–2000 to develop baits for delivering an oral rabies vaccine to raccoons (Procyon lotor) and coyotes (Canis latrans). A bait was sought that: (1) was attractive to the target species, (2) could be distributed by aircraft, (3) was as effective (or more so) than the currently used fish meal polymer bait, and (4) could be produced in large numbers by automated procedures and could be purchased by user groups at substantially lower cost.

Ten field trials were conducted to document raccoons’ bait flavor preferences, evaluate a new vaccine sachet bait coated with …

Cellular Tropisms And Co-Receptor Usage Of Hiv-1 Isolates From Vertically Infected Children With Neurological Abnormalities And Rapid Disease Progression, Micheline Mccarthy, Jun He, Denise Auger, Rebeca Geffin, Cecilia Hutto, Charles Wood, Gwendolyn Scott

Nebraska Center for Virology: Faculty Publications

The longitudinal evolution of HIV-1 phenotypes was studied in a cohort of six vertically infected children with early onset and rapid progression of clinical disease. Among 30 viral isolates obtained from peripheral blood, tropisms for both human blood-derived cells (macrophages, T-lymphocytes), and for human neural (brain-derived) cells (microglia, astrocytes) were determined, as was chemokine co-receptor usage. All children harbored from birth macrophage-tropic isolates using the CCR5 co-receptor. Two children later developed T-cell tropic isolates with CXCR4 and CCR3 usage. While all six patients developed neurological abnormalities, only three produced neural cell tropic isolates, which used CCR5. However, early and persistent …

Femtosecond Laser Utilization Methods And Apparatus And Method For Producing Nanoparticles, Dennis R. Alexander

Department of Electrical and Computer Engineering: Faculty Publications

The present invention teaches various femtosecond machining and drilling apparatus and processes for fabricating tools and the like from both traditional and non-traditional materials. Also described are novel tools such as scalpels, and nozzles fabricated from the apparatus and processes of the present invention. Likewise, the present invention may be utilized in both a novel propulsion system and the production of materials formed from nanometer sized particles and the like.

Small Glutamine-Rich Protein/Viral Protein U–Binding Protein Is A Novel Cochaperone That Affects Heat Shock Protein 70 Activity, Peter C. Angeletti, Doriann Walker, Antonito T. Panganiban

Nebraska Center for Virology: Faculty Publications

Molecular chaperone complexes containing heat shock protein (Hsp) 70 and Hsp90 are regulated by cochaperones, including a subclass of regulators, such as Hsp70 interacting protein (Hip), C-terminus of Hsp70 interacting protein (CHIP), and Hsp70-Hsp90 organizing factor (Hop), that contain tetratricopeptide repeats (TPRs), where Hsp70 refers to Hsp70 and its nearly identical constitutive counterpart, Hsc70, together. These proteins interact with the Hsp70 to regulate adenosine triphosphatase (ATPase) and folding activities or to generate the chaperone complex. Here we provide evidence that small glutamine-rich protein/viral protein U–binding protein (SGT/UBP) is a cochaperone that negatively regulates Hsp70. By “Far-Western” and pull-down assays, SGT/UBP …

Mycobacterium Smegmatis D-Alanine Racemase Mutants Are Not Dependent On D-Alanine For Growth, Ofelia Chacon, Zhengyu Feng, N. Beth Harris , Nancy E. Caceres, Garry Adams, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

Mycobacterium smegmatis is a fast-growing nonpathogenic species particularly useful in studying basic cellular processes of relevance to pathogenic mycobacteria. This study focused on the D-alanine racemase gene (alrA), which is involved in the synthesis of D-alanine, a basic component of peptidoglycan that forms the backbone of the cell wall. M. smegmatis alrA null mutants were generated by homologous recombination using a kanamycin resistance marker for insertional inactivation. Mutants were selected on Middlebrook medium supplemented with 50 mM D-alanine and 20 μg of kanamycin per ml. These mutants were also able to grow in standard and minimal media without …

Technology, Libraries And The Internet: A Comparison Of The Impact Of The Printing Press And World Wide Web, Gentry Lankewicz Holbert

E-JASL: Electronic Journal of Academic and Special Librarianship (1999-2009, Volumes 1-10)

In thinking about technology and libraries, what struck me most is that we are living and experiencing a great turning point in history. "May you live in interesting times" is an ancient Chinese curse. Interesting times indeed, with all their glory, advantages, and pitfalls. We are still in the process of discovering what to do with new technology in the dissemination of information.

With the dawn of the Internet, computer automation, and intricate databases, I feel that, as a librarian, I am at the helm of the true information age. And how interesting to be here at the Vernadsky Library, …

A Procedural Evaluation Of An Analytic-Deliberative Process: The Columbia River Comprehensive Impact Assessment, Aimee Guglielmo Kinney, Thomas M. Leschine

United States Army Corps of Engineers, Omaha District

The U.S. Department of Energy's Columbia River Comprehensive Impact Assessment (CRCIA) was an ambitious attempt to direct its cleanup of the Hanford Nuclear Reservation toward the most significant risks to the Columbia River resulting from past plutonium production. DOE's approach was uncommonly open, including tribal, regulatory agency, and other Hanford interest group representatives on the board that was to develop the assessment approach. The CRCIA process had attributes of the ``analytic-deliberative'' process for risk assessment recommended by the National Research Council. Nevertheless, differences between the DOE and other participants over what was meant by the term ``comprehensive'' in the group's …

Human Immunodeficiency Virus Type 1 (Hiv-1) Tat Induces Nitric-Oxide Synthase In Human Astroglia, Xiaojuan Liu, Malabendu Jana, Subhajit Dasgupta, Sreenivas Koka, Jun He, Charles Wood, Kalipada Pahan

Nebraska Center for Virology: Faculty Publications

Human immunodeficiency virus type 1 (HIV-1) infection is known to cause neuronal injury and dementia in a significant proportion of patients. However, the mechanism by which HIV-1 mediates its deleterious effects in the brain is poorly defined. The present study was undertaken to investigate the effect of the HIV-1 tat gene on the expression of inducible nitric-oxide synthase (iNOS) in human U373MG astroglial cells and primary astroglia. Expression of the tat gene as RSV- tat but not that of the CAT gene as RSV-CAT in U373MG astroglial cells led to the induction of NO production and the expression of iNOS …

Effects Of Management Practices On Grassland Birds: Burrowing Owl, Jill A. Dechant, Marriah L. Sondreal, Douglas H. Johnson, Lawrence D. Igl, Christopher M. Goldade, Paul A. Rabie, Betty R. Euliss

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Burrowing Owl ( Speotyto cunicularia hypugaea):
Breeding range
Suitable habitat
Prey habitat
Area requirements
Brown-headed Cowbird brood parasitism
Breeding-season phenology and site fidelity
Species’ response to management
Management Recommendations
Characteristics

Intercontinental Migration Of Neogene Amphicyonids (Mammalia, Carnivora): Appearance Of The Eurasian Beardog Ysengrinia In North America, Robert M. Hunt Jr.

Department of Earth and Atmospheric Sciences: Faculty Publications

At the beginning of the Neogene a remarkable faunal turnover occurred within the North American carnivore community. The dominant larger Oligocene carnivores (creodonts, nimravid cats, the amphicyonid Daphoenus) became extinct during the late Oligocene and were replaced in the early Miocene by amphicyonine amphicyonids and hemicyonine ursids that entered North America from Eurasia. During a five million-year interval from ~23 to 18 Ma, large amphicyonines appear in late Arikareean and early Hemingfordian faunas of the North American midcontinent. Although most fossils are from western Nebraska and southeastern Wyoming, occurrences of amphicyonines at several sites in the eastern United States …

"Introduction" To The Shape Of Change: Essays In Early Modern Literature And La Fontaine In Honor Of David Lee Rubin, Anne L. Birberick, Russell J. Ganim

Department of Modern Languages and Literatures: Faculty Publications

Since Michel Foucault’s The Archeology of Knowledge gained widespread critical recognition in the late 1960s, the concept of change has held a privileged position in the discourse of literary and cultural studies. Concerned with disruptions, transformations, and upheavals, scholars have focused on those moments of rupture when the present breaks with the past, when originality displaces convention, when inventio repudiates imitatio. This emphasis on aesthetic and epistemic shifts has all too often resulted in the neglect of more traditional issues having to do with problems of cultural development, evolution, and influence. It has also overlooked the idea of reading and …

Chemical Partitioning During Crystallization And Its Effect On The Microstructure And Magnetic Behavior Of Modified Nd–Fe–B Permanent Magnets, Jeffrey E. Shield, B. B. Kappes, D. J. Branagan, J. Bentley

Department of Mechanical and Materials Engineering: Faculty Publications

In this paper, the effect of alloy composition on the microstructural development of devitrified Nd–Fe–B-based alloys is investigated. While crystallization of simple ternary Nd₂Fe₁₄B resulted in a highly variable microstructure and relatively poor demagnetization behavior, alloying additions, notably Ti and C, were found to significantly refine and homogenize the crystallized microstructure. High spatial resolution energy dispersive X-ray spectroscopy revealed that partitioning to the grain boundaries led to the grain refinement by slowing interface kinetics. The more homogeneous grain structure resulted in improved demagnetization behavior, but segregating elements isolated grains and limited intergranular exchange coupling.

What Are We Doing To Prevent Entry Of Potentially Catastrophic Foreign Animal Diseases Into The Usa, Gary C. Smith

Range Beef Cow Symposium

What are Foreign Animal Diseases? By definition, a Foreign Animal Disease (FAD) is one which originates in another country and can, or does, enter the USA. An agency of our federal government, the Animal and Plant Health Inspection Service (APHIS) of the U.S. Department of Agriculture (USDA), is charged with responsibility for preventing entry of FADs into our country and is especially vigilant regarding those FADs which would have potentially catastrophic effects on farm-animal populations, the agricultural economy and/or the public (human) health. Activities of APHIS specifically related to FADs include (USDA/APHIS, 2001): (a) Guarding our borders against foreign agricultural …